FvH4_4g12550

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000071
Physical Location & Seq
Forward (+)
16237763 .. 16241664
3902 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12550.t1

Sequence Viewer

Length: 1578 bp
ATGAAGAGGATTGGATACCGACAAAGTAATTCAGATCACACTTTGTTTCTTAAACACCGATGTGGTAAGGTGACAGCTTTAATTATTTATGTAGATGATATGGTTGTGACAGGTGATGATAATGAGGAAATTCAAAGGTTACAAGATATGTTGTCTTCTGAATTTGAGATGAAGGATTTGGGTAGTCTAAAGTACTTCTTGGGAATTGAAGTTGCTCGTGGGAAGGATTGTATTGTGCTGAGTCAGAGGAAGTATGTCCTAGATTTGTTAGCAGAAACGGGTATGCTTGATTGTAAACCGGCTGATACTCCTATTGAGCAAAACCATTGGTTAGCAGAGTATTTGGATCAAGTGCCTACAAATAAGGCGCGATATCAGAGGTTAGTTGGCCAGTTGATTTATTTGTCTCACACCAGACCAGATTTAGCCTATGTAGTTAGTGTGGTGAGTCAGTTTATGCATAATCCTAGTGAGGCTCATATGGAAGCTGTATTTCGTATTTTGAAGTACTTGAAGTCTGCTCCAGGGAAGGGATTAATTTTTTCAAAACACAAGCACTTGGCTGTTTTTGGGTACACAGACGCAGACTGGGCAGGTAATATTACAGATCGCATGTCTACTTCAGGCTACTTCACTTTCGTTGGTGGTAATTTGGTTACTTGGAAAAGTAAGAAGCAAAATGTGGTAGCTCGTTCTAGTGCTGAAGCAGAATATAGAGGAATGGCTCGAGGACTTTGTGAGATGTTGTGGTTGGGACACTTGTTGAGAGATTTGGGATTTCGGCAAAAGAAGGCTATGCCGCTTTATTGTGATAATAAGGCTGCTATTGAAATTGCTCACAATCCTGTTCAACATGATCGCACAAAGCACGTGGAGCTTTCTCTAGCATTGGAGGCAGAATTGTTGACTGCGATACAGGAGGCAAATCACCTTATTTCTGCCCCTGTCTTTTCTCCAAGCTTTTATGCCACTGACCCAGTAAAACTTGAAGTGGCCAAAGACATAGTTCAAAGTATTCTGAAGTGTGACCTTGAAGCCTTACTGGACAAACAGTGTTACAAAGATTTGAAAGAAGCTTTCAAGTATCTGTCCTCTATTAGTTTTTTTGATCCAATCATGGTCCTAAGACTTGACTGGATGCTGAACGATGTGGAGACTGAACTACCACACTATAAAGAAGCTATAGAAGAATGTGTTACAGGTAGAGAACAGGCAAAGGACCTTCAACGTGCGGTAATATCTCTGAAAGATAAGCTGGAAGCTGCTTGTGCTACAAAACCTAGAATTATTGAACTTGAAAATGAGCTGCATGATCTTGAGTATCAAATTGAGGCTCTTCAGGCTCAATTTGCAGATTGTCAGAGCCAGAAAGAAGTCCTCCAGGCAAAATTGGATGCTGAATTGGATCAGCTTCGTCCAGAAAAGGAGGCACTCCTGGGTAAATGCAAGTTGTTTACTGCTTTCAAAGAGAGATTGCCAGAAATGAAGAATCGGATCAACGCAGGGATTGGCGTGTGGGAAGCCTTTAAGCCAGCTTTTAATATCCAATTTAGAGGTGTTAACCAACTAAGCCATTAG

Protein Analysis

526

Amino Acids

59.84

Weight (kDa)

6.18

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 3 - 106 2.5e-19 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 584
AccI GTMKAC 1 cut(s) 617
AccII CGCG 1 cut(s) 370
AciI CCGC 2 cut(s) 800, 1232
AclWI GGATC 4 cut(s) 354, 1103, 1413, 1502
AcoI YGGCCR 2 cut(s) 388, 993
AcsI RAATTY 2 cut(s) 129, 161
AcuI CTGAAG 4 cut(s) 606, 723, 1040, 1322
AcvI CACGTG 1 cut(s) 871
AfaI GTAC 3 cut(s) 194, 509, 575
AfiI CCNNNNNNNGG 1 cut(s) 530
AjnI CCWGG 3 cut(s) 523, 1380, 1434
AleI CACNNNNGTG 1 cut(s) 60
Alw26I GTCTC 2 cut(s) 411, 1148
AlwI GGATC 4 cut(s) 354, 1103, 1413, 1502
Ama87I CYCGRG 1 cut(s) 726
AoxI GGCC 2 cut(s) 388, 993
ApeKI GCWGC 3 cut(s) 821, 1262, 1306
ApoI RAATTY 2 cut(s) 129, 161
AseI ATTAAT 1 cut(s) 536
AspLEI GCGC 1 cut(s) 370
AspS9I GGNCC 2 cut(s) 1120, 1219
AsuHPI GGTGA 4 cut(s) 82, 125, 457, 920
AvaI CYCGRG 1 cut(s) 726
AvaII GGWCC 2 cut(s) 1120, 1219
BalI TGGCCA 2 cut(s) 390, 995
BauI CACGAG 1 cut(s) 216
BbrPI CACGTG 1 cut(s) 871
BbsI GAAGAC 1 cut(s) 147
BbvI GCAGC 3 cut(s) 808, 1249, 1293
BciT130I CCWGG 3 cut(s) 525, 1382, 1436
BciVI GTATCC 1 cut(s) 8
BcoDI GTCTC 2 cut(s) 411, 1148
BfaI CTAG 5 cut(s) 260, 468, 696, 884, 1281
BfmI CTRYAG 1 cut(s) 1182
BfuAI ACCTGC 1 cut(s) 584
BfuI GTATCC 1 cut(s) 8
BisI GCNGC 4 cut(s) 800, 822, 1263, 1307
BlsI GCNGC 4 cut(s) 801, 823, 1264, 1308
BmcAI AGTACT 2 cut(s) 194, 509
Bme1390I CCNGG 3 cut(s) 525, 1382, 1436
Bme18I GGWCC 2 cut(s) 1120, 1219
BmeT110I CYCGRG 1 cut(s) 726
BmgT120I GGNCC 2 cut(s) 1120, 1219
BmrFI CCNGG 3 cut(s) 525, 1382, 1436
BmrI ACTGGG 2 cut(s) 598, 971
BmsI GCATC 2 cut(s) 1128, 1384
BmuI ACTGGG 2 cut(s) 598, 971
BpiI GAAGAC 1 cut(s) 147
BpmI CTGGAG 2 cut(s) 507, 1364
BpuEI CTTGAG 1 cut(s) 1337
BsaAI YACGTR 1 cut(s) 871
BsaJI CCNNGG 2 cut(s) 524, 1435
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse118I RCCGGY 1 cut(s) 298
Bse1I ACTGG 5 cut(s) 391, 593, 977, 1047, 1139
BseBI CCWGG 3 cut(s) 525, 1382, 1436
BseDI CCNNGG 2 cut(s) 524, 1435
BseGI GGATG 2 cut(s) 1143, 1399
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 5 cut(s) 391, 593, 977, 1047, 1139
BseXI GCAGC 3 cut(s) 808, 1249, 1293
Bsh1236I CGCG 1 cut(s) 370
BshFI GGCC 2 cut(s) 390, 995
BsiHKCI CYCGRG 1 cut(s) 726
BsiSI CCGG 1 cut(s) 299
BslFI GGGAC 1 cut(s) 768
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 2 cut(s) 411, 1148
BsmFI GGGAC 1 cut(s) 768
BsnI GGCC 2 cut(s) 390, 995
BsoBI CYCGRG 1 cut(s) 726
Bsp143I GATC 8 cut(s) 34, 346, 607, 856, 1108, 1312, 1405, 1494
BspACI CCGC 2 cut(s) 800, 1232
BspANI GGCC 2 cut(s) 390, 995
BspCNI CTCAG 1 cut(s) 231
BspFNI CGCG 1 cut(s) 370
BspMI ACCTGC 1 cut(s) 584
BspPI GGATC 4 cut(s) 354, 1103, 1413, 1502
BspQI GCTCTTC 1 cut(s) 1341
BsrFI RCCGGY 1 cut(s) 298
BsrI ACTGG 5 cut(s) 391, 593, 977, 1047, 1139
BssAI RCCGGY 1 cut(s) 298
BssECI CCNNGG 2 cut(s) 524, 1435
BssMI GATC 8 cut(s) 34, 346, 607, 856, 1108, 1312, 1405, 1494
BssSI CACGAG 1 cut(s) 216
Bst2BI CACGAG 1 cut(s) 216
Bst2UI CCWGG 3 cut(s) 525, 1382, 1436
Bst4CI ACNGT 1 cut(s) 1053
Bst6I CTCTTC 1 cut(s) 1341
BstBAI YACGTR 1 cut(s) 871
BstC8I GCNNGC 1 cut(s) 1533
BstDEI CTNAG 3 cut(s) 239, 1124, 1568
BstF5I GGATG 2 cut(s) 1143, 1399
BstFNI CGCG 1 cut(s) 370
BstHHI GCGC 1 cut(s) 370
BstKTI GATC 8 cut(s) 37, 349, 610, 859, 1111, 1315, 1408, 1497
BstMAI GTCTC 2 cut(s) 411, 1148
BstMBI GATC 8 cut(s) 34, 346, 607, 856, 1108, 1312, 1405, 1494
BstMWI GCNNNNNNNGC 6 cut(s) 590, 874, 893, 1268, 1340, 1349
BstNI CCWGG 3 cut(s) 525, 1382, 1436
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 3 cut(s) 523, 1380, 1434
BstSFI CTRYAG 1 cut(s) 1182
BstUI CGCG 1 cut(s) 370
BstV1I GCAGC 3 cut(s) 808, 1249, 1293
BstV2I GAAGAC 1 cut(s) 147
BsuI GTATCC 1 cut(s) 8
BsuRI GGCC 2 cut(s) 390, 995
BtsCI GGATG 2 cut(s) 1143, 1399
BtsIMutI CAGTG 2 cut(s) 969, 1058
BveI ACCTGC 1 cut(s) 584
Cac8I GCNNGC 1 cut(s) 1533
CfoI GCGC 1 cut(s) 370
Cfr10I RCCGGY 1 cut(s) 298
Cfr13I GGNCC 2 cut(s) 1120, 1219
CseI GACGC 1 cut(s) 590
Csp6I GTAC 3 cut(s) 193, 508, 574
CspCI CAANNNNNGTGG 2 cut(s) 852, 887
CviAII CATG 4 cut(s) 613, 854, 1117, 1310
CviQI GTAC 3 cut(s) 193, 508, 574
DdeI CTNAG 3 cut(s) 239, 1124, 1568
DpnI GATC 8 cut(s) 36, 348, 609, 858, 1110, 1314, 1407, 1496
DpnII GATC 8 cut(s) 34, 346, 607, 856, 1108, 1312, 1405, 1494
EaeI YGGCCR 2 cut(s) 388, 993
Eam1104I CTCTTC 1 cut(s) 1341
EarI CTCTTC 1 cut(s) 1341
Eco32I GATATC 1 cut(s) 374
Eco47I GGWCC 2 cut(s) 1120, 1219
Eco57I CTGAAG 4 cut(s) 606, 723, 1040, 1322
Eco72I CACGTG 1 cut(s) 871
Eco88I CYCGRG 1 cut(s) 726
EcoO109I RGGNCCY 1 cut(s) 1219
EcoRII CCWGG 3 cut(s) 523, 1380, 1434
EcoRV GATATC 1 cut(s) 374
EcoT22I ATGCAT 1 cut(s) 462
FaeI CATG 4 cut(s) 616, 857, 1120, 1313
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 1 cut(s) 768
FatI CATG 4 cut(s) 612, 853, 1116, 1309
FauNDI CATATG 1 cut(s) 480
FblI GTMKAC 1 cut(s) 617
Fnu4HI GCNGC 4 cut(s) 800, 822, 1263, 1307
FokI GGATG 2 cut(s) 1150, 1406
Fsp4HI GCNGC 4 cut(s) 800, 822, 1263, 1307
FspBI CTAG 5 cut(s) 260, 468, 696, 884, 1281
GlaI GCGC 1 cut(s) 369
GluI GCNGC 4 cut(s) 800, 822, 1263, 1307
GsuI CTGGAG 2 cut(s) 507, 1364
HaeIII GGCC 2 cut(s) 390, 995
HapII CCGG 1 cut(s) 299
HgaI GACGC 1 cut(s) 590
HhaI GCGC 1 cut(s) 370
Hin1II CATG 4 cut(s) 616, 857, 1120, 1313
Hin6I GCGC 1 cut(s) 368
HinP1I GCGC 1 cut(s) 368
HincII GTYRAC 2 cut(s) 906, 1561
HindII GTYRAC 2 cut(s) 906, 1561
HindIII AAGCTT 2 cut(s) 958, 1074
HinfI GANTC 3 cut(s) 241, 448, 1489
HpaI GTTAAC 1 cut(s) 1561
HpaII CCGG 1 cut(s) 299
HphI GGTGA 4 cut(s) 82, 125, 457, 920
Hpy166II GTNNAC 6 cut(s) 296, 576, 618, 906, 1455, 1561
Hpy188I TCNGA 8 cut(s) 34, 160, 246, 378, 1020, 1245, 1362, 1494
Hpy188III TCNNGA 2 cut(s) 1316, 1418
Hpy8I GTNNAC 6 cut(s) 296, 576, 618, 906, 1455, 1561
HpyAV CCTTC 5 cut(s) 166, 217, 523, 784, 1232
HpyCH4III ACNGT 1 cut(s) 1053
HpyCH4IV ACGT 2 cut(s) 870, 1228
HpyCH4V TGCA 4 cut(s) 460, 1309, 1352, 1446
HpyF10VI GCNNNNNNNGC 6 cut(s) 590, 874, 893, 1268, 1340, 1349
HpyF3I CTNAG 3 cut(s) 239, 1124, 1568
HpySE526I ACGT 2 cut(s) 870, 1228
Hsp92II CATG 4 cut(s) 616, 857, 1120, 1313
HspAI GCGC 1 cut(s) 368
KspAI GTTAAC 1 cut(s) 1561
Kzo9I GATC 8 cut(s) 34, 346, 607, 856, 1108, 1312, 1405, 1494
LguI GCTCTTC 1 cut(s) 1341
LmnI GCTCC 2 cut(s) 526, 874
Lsp1109I GCAGC 3 cut(s) 808, 1249, 1293
LweI GCATC 2 cut(s) 1128, 1384
MaeI CTAG 5 cut(s) 260, 468, 696, 884, 1281
MaeII ACGT 2 cut(s) 870, 1228
MaeIII GTNAC 7 cut(s) 70, 106, 138, 655, 1025, 1055, 1195
MalI GATC 8 cut(s) 36, 348, 609, 858, 1110, 1314, 1407, 1496
MboI GATC 8 cut(s) 34, 346, 607, 856, 1108, 1312, 1405, 1494
MboII GAAGA 5 cut(s) 16, 147, 1199, 1328, 1498
MlsI TGGCCA 2 cut(s) 390, 995
MluNI TGGCCA 2 cut(s) 390, 995
MlyI GAGTC 2 cut(s) 250, 457
Mox20I TGGCCA 2 cut(s) 390, 995
Mph1103I ATGCAT 1 cut(s) 462
MscI TGGCCA 2 cut(s) 390, 995
MseI TTAA 6 cut(s) 51, 80, 536, 1527, 1539, 1560
MslI CAYNNNNRTG 1 cut(s) 60
Msp20I TGGCCA 2 cut(s) 390, 995
MspI CCGG 1 cut(s) 299
MspR9I CCNGG 3 cut(s) 525, 1382, 1436
MvaI CCWGG 3 cut(s) 525, 1382, 1436
MvnI CGCG 1 cut(s) 370
MwoI GCNNNNNNNGC 6 cut(s) 590, 874, 893, 1268, 1340, 1349
NdeI CATATG 1 cut(s) 480
NdeII GATC 8 cut(s) 34, 346, 607, 856, 1108, 1312, 1405, 1494
NlaIII CATG 4 cut(s) 616, 857, 1120, 1313
NmuCI GTSAC 3 cut(s) 70, 106, 1025
NsiI ATGCAT 1 cut(s) 462
NspI RCATGY 1 cut(s) 616
OliI CACNNNNGTG 1 cut(s) 60
PaeR7I CTCGAG 1 cut(s) 726
PciSI GCTCTTC 1 cut(s) 1341
PfeI GAWTC 1 cut(s) 1489
PkrI GCNGC 4 cut(s) 801, 823, 1264, 1308
PleI GAGTC 2 cut(s) 249, 456
PmaCI CACGTG 1 cut(s) 871
PmlI CACGTG 1 cut(s) 871
PpsI GAGTC 2 cut(s) 249, 456
Ppu21I YACGTR 1 cut(s) 871
PpuMI RGGWCCY 1 cut(s) 1219
PshBI ATTAAT 1 cut(s) 536
Psp5II RGGWCCY 1 cut(s) 1219
Psp6I CCWGG 3 cut(s) 523, 1380, 1434
PspCI CACGTG 1 cut(s) 871
PspGI CCWGG 3 cut(s) 523, 1380, 1434
PspPI GGNCC 2 cut(s) 1120, 1219
PspPPI RGGWCCY 1 cut(s) 1219
PspXI VCTCGAGB 1 cut(s) 726
RsaI GTAC 3 cut(s) 194, 509, 575
RsaNI GTAC 3 cut(s) 193, 508, 574
RseI CAYNNNNRTG 1 cut(s) 60
SapI GCTCTTC 1 cut(s) 1341
SaqAI TTAA 6 cut(s) 51, 80, 536, 1527, 1539, 1560
SatI GCNGC 4 cut(s) 800, 822, 1263, 1307
Sau3AI GATC 8 cut(s) 34, 346, 607, 856, 1108, 1312, 1405, 1494
Sau96I GGNCC 2 cut(s) 1120, 1219
ScaI AGTACT 2 cut(s) 194, 509
SchI GAGTC 2 cut(s) 250, 457
ScrFI CCNGG 3 cut(s) 525, 1382, 1436
SfaNI GCATC 2 cut(s) 1128, 1384
SfcI CTRYAG 1 cut(s) 1182
Sfr274I CTCGAG 1 cut(s) 726
SinI GGWCC 2 cut(s) 1120, 1219
SlaI CTCGAG 1 cut(s) 726
SmiMI CAYNNNNRTG 1 cut(s) 60
SmlI CTYRAG 2 cut(s) 726, 1316
SmoI CTYRAG 2 cut(s) 726, 1316
SsiI CCGC 2 cut(s) 800, 1232
SspI AATATT 1 cut(s) 601
SspMI CTAG 5 cut(s) 260, 468, 696, 884, 1281
StyD4I CCNGG 3 cut(s) 523, 1380, 1434
TaaI ACNGT 1 cut(s) 1053
TaiI ACGT 2 cut(s) 873, 1231
TaqI TCGA 1 cut(s) 727
TatI WGTACW 2 cut(s) 192, 507
TauI GCSGC 1 cut(s) 802
TfiI GAWTC 1 cut(s) 1489
Tru1I TTAA 6 cut(s) 51, 80, 536, 1527, 1539, 1560
Tru9I TTAA 6 cut(s) 51, 80, 536, 1527, 1539, 1560
TscAI CASTG 2 cut(s) 976, 1058
TseFI GTSAC 3 cut(s) 70, 106, 1025
TseI GCWGC 3 cut(s) 821, 1262, 1306
Tsp45I GTSAC 3 cut(s) 70, 106, 1025
TspDTI ATGAA 3 cut(s) 17, 185, 1499
TspRI CASTG 2 cut(s) 976, 1058
VpaK11BI GGWCC 2 cut(s) 1120, 1219
VspI ATTAAT 1 cut(s) 536
XapI RAATTY 2 cut(s) 129, 161
XceI RCATGY 1 cut(s) 616
XhoI CTCGAG 1 cut(s) 726
XmiI GTMKAC 1 cut(s) 617
XspI CTAG 5 cut(s) 260, 468, 696, 884, 1281
ZrmI AGTACT 2 cut(s) 194, 509
Zsp2I ATGCAT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.