Reverse transcriptase (RNA-dependent DNA polymerase)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 238 |
| AcsI | RAATTY | 1 cut(s) | 69 |
| AcyI | GRCGYC | 1 cut(s) | 239 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 248 |
| AgsI | TTSAA | 1 cut(s) | 232 |
| AjnI | CCWGG | 1 cut(s) | 455 |
| AluBI | AGCT | 4 cut(s) | 259, 296, 431, 474 |
| AluI | AGCT | 4 cut(s) | 259, 296, 431, 474 |
| Alw26I | GTCTC | 1 cut(s) | 17 |
| ApeKI | GCWGC | 1 cut(s) | 296 |
| ApoI | RAATTY | 1 cut(s) | 69 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 464 |
| AspLEI | GCGC | 1 cut(s) | 241 |
| BanI | GGYRCC | 1 cut(s) | 238 |
| BbvI | GCAGC | 1 cut(s) | 283 |
| BccI | CCATC | 1 cut(s) | 229 |
| BciT130I | CCWGG | 1 cut(s) | 457 |
| BclI | TGATCA | 1 cut(s) | 64 |
| BcoDI | GTCTC | 1 cut(s) | 17 |
| BfaI | CTAG | 3 cut(s) | 132, 186, 248 |
| BfmI | CTRYAG | 1 cut(s) | 10 |
| BfoI | RGCGCY | 1 cut(s) | 242 |
| BglII | AGATCT | 1 cut(s) | 244 |
| BisI | GCNGC | 1 cut(s) | 297 |
| BlsI | GCNGC | 1 cut(s) | 298 |
| Bme1390I | CCNGG | 1 cut(s) | 457 |
| BmiI | GGNNCC | 1 cut(s) | 240 |
| BmrFI | CCNGG | 1 cut(s) | 457 |
| BmsI | GCATC | 1 cut(s) | 193 |
| BoxI | GACNNNNGTC | 1 cut(s) | 12 |
| BsaBI | GATNNNNATC | 1 cut(s) | 500 |
| BsaHI | GRCGYC | 1 cut(s) | 239 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 248 |
| Bse1I | ACTGG | 1 cut(s) | 320 |
| Bse8I | GATNNNNATC | 1 cut(s) | 500 |
| BseBI | CCWGG | 1 cut(s) | 457 |
| BseGI | GGATG | 1 cut(s) | 208 |
| BseJI | GATNNNNATC | 1 cut(s) | 500 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 248 |
| BseMII | CTCAG | 2 cut(s) | 429, 489 |
| BseNI | ACTGG | 1 cut(s) | 320 |
| BseXI | GCAGC | 1 cut(s) | 283 |
| BshNI | GGYRCC | 1 cut(s) | 238 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 248 |
| BsmAI | GTCTC | 1 cut(s) | 17 |
| Bsp143I | GATC | 2 cut(s) | 64, 244 |
| BspCNI | CTCAG | 2 cut(s) | 428, 488 |
| BspLI | GGNNCC | 1 cut(s) | 240 |
| BspMAI | CTGCAG | 1 cut(s) | 14 |
| BspQI | GCTCTTC | 1 cut(s) | 414 |
| BspT107I | GGYRCC | 1 cut(s) | 238 |
| BsrI | ACTGG | 1 cut(s) | 320 |
| BssMI | GATC | 2 cut(s) | 64, 244 |
| BssNI | GRCGYC | 1 cut(s) | 239 |
| Bst2UI | CCWGG | 1 cut(s) | 457 |
| Bst4CI | ACNGT | 1 cut(s) | 359 |
| Bst6I | CTCTTC | 1 cut(s) | 414 |
| BstACI | GRCGYC | 1 cut(s) | 239 |
| BstDEI | CTNAG | 3 cut(s) | 260, 415, 475 |
| BstF5I | GGATG | 1 cut(s) | 208 |
| BstH2I | RGCGCY | 1 cut(s) | 242 |
| BstHHI | GCGC | 1 cut(s) | 241 |
| BstKTI | GATC | 2 cut(s) | 67, 247 |
| BstMAI | GTCTC | 1 cut(s) | 17 |
| BstMBI | GATC | 2 cut(s) | 64, 244 |
| BstNI | CCWGG | 1 cut(s) | 457 |
| BstNSI | RCATGY | 1 cut(s) | 347 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 12 |
| BstSCI | CCNGG | 1 cut(s) | 455 |
| BstSFI | CTRYAG | 1 cut(s) | 10 |
| BstV1I | GCAGC | 1 cut(s) | 283 |
| BstX2I | RGATCY | 1 cut(s) | 244 |
| BstYI | RGATCY | 1 cut(s) | 244 |
| BtsCI | GGATG | 1 cut(s) | 208 |
| CfoI | GCGC | 1 cut(s) | 241 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 327, 362 |
| CviAII | CATG | 3 cut(s) | 329, 344, 493 |
| CviJI | RGCY | 4 cut(s) | 259, 296, 431, 474 |
| CviKI_1 | RGCY | 4 cut(s) | 259, 296, 431, 474 |
| DdeI | CTNAG | 3 cut(s) | 260, 415, 475 |
| DinI | GGCGCC | 1 cut(s) | 240 |
| DpnI | GATC | 2 cut(s) | 66, 246 |
| DpnII | GATC | 2 cut(s) | 64, 244 |
| Eam1104I | CTCTTC | 1 cut(s) | 414 |
| EarI | CTCTTC | 1 cut(s) | 414 |
| Eco32I | GATATC | 1 cut(s) | 502 |
| EcoRII | CCWGG | 1 cut(s) | 455 |
| EcoRV | GATATC | 1 cut(s) | 502 |
| EcoT22I | ATGCAT | 1 cut(s) | 180 |
| EgeI | GGCGCC | 1 cut(s) | 240 |
| EheI | GGCGCC | 1 cut(s) | 240 |
| FaeI | CATG | 3 cut(s) | 332, 347, 496 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 363, 395 |
| FatI | CATG | 3 cut(s) | 328, 343, 492 |
| FauNDI | CATATG | 1 cut(s) | 198 |
| FbaI | TGATCA | 1 cut(s) | 64 |
| Fnu4HI | GCNGC | 1 cut(s) | 297 |
| FokI | GGATG | 1 cut(s) | 215 |
| Fsp4HI | GCNGC | 1 cut(s) | 297 |
| FspBI | CTAG | 3 cut(s) | 132, 186, 248 |
| GlaI | GCGC | 1 cut(s) | 240 |
| GluI | GCNGC | 1 cut(s) | 297 |
| HaeII | RGCGCY | 1 cut(s) | 242 |
| HhaI | GCGC | 1 cut(s) | 241 |
| Hin1I | GRCGYC | 1 cut(s) | 239 |
| Hin1II | CATG | 3 cut(s) | 332, 347, 496 |
| Hin6I | GCGC | 1 cut(s) | 239 |
| HinP1I | GCGC | 1 cut(s) | 239 |
| HincII | GTYRAC | 2 cut(s) | 355, 481 |
| HindII | GTYRAC | 2 cut(s) | 355, 481 |
| HindIII | AAGCTT | 1 cut(s) | 257 |
| HinfI | GANTC | 1 cut(s) | 40 |
| Hpy166II | GTNNAC | 3 cut(s) | 355, 412, 481 |
| Hpy188I | TCNGA | 2 cut(s) | 216, 418 |
| Hpy188III | TCNNGA | 2 cut(s) | 62, 380 |
| Hpy8I | GTNNAC | 3 cut(s) | 355, 412, 481 |
| HpyAV | CCTTC | 2 cut(s) | 81, 102 |
| HpyCH4III | ACNGT | 1 cut(s) | 359 |
| HpyCH4IV | ACGT | 1 cut(s) | 97 |
| HpyCH4V | TGCA | 7 cut(s) | 12, 178, 206, 280, 299, 311, 509 |
| HpyF3I | CTNAG | 3 cut(s) | 260, 415, 475 |
| HpySE526I | ACGT | 1 cut(s) | 97 |
| Hsp92I | GRCGYC | 1 cut(s) | 239 |
| Hsp92II | CATG | 3 cut(s) | 332, 347, 496 |
| HspAI | GCGC | 1 cut(s) | 239 |
| KasI | GGCGCC | 1 cut(s) | 238 |
| Ksp22I | TGATCA | 1 cut(s) | 64 |
| Kzo9I | GATC | 2 cut(s) | 64, 244 |
| LguI | GCTCTTC | 1 cut(s) | 414 |
| LpnPI | CCDG | 6 cut(s) | 75, 150, 255, 333, 442, 469 |
| Lsp1109I | GCAGC | 1 cut(s) | 283 |
| LweI | GCATC | 1 cut(s) | 193 |
| MaeI | CTAG | 3 cut(s) | 132, 186, 248 |
| MaeII | ACGT | 1 cut(s) | 97 |
| MaeIII | GTNAC | 1 cut(s) | 251 |
| MalI | GATC | 2 cut(s) | 66, 246 |
| MboI | GATC | 2 cut(s) | 64, 244 |
| MboII | GAAGA | 4 cut(s) | 203, 244, 431, 472 |
| MfeI | CAATTG | 2 cut(s) | 18, 300 |
| MflI | RGATCY | 1 cut(s) | 244 |
| MluCI | AATT | 3 cut(s) | 18, 69, 300 |
| Mly113I | GGCGCC | 1 cut(s) | 239 |
| MmeI | TCCRAC | 1 cut(s) | 98 |
| MnlI | CCTC | 2 cut(s) | 216, 343 |
| Mph1103I | ATGCAT | 1 cut(s) | 180 |
| MroXI | GAANNNNTTC | 1 cut(s) | 464 |
| MseI | TTAA | 1 cut(s) | 369 |
| MslI | CAYNNNNRTG | 2 cut(s) | 35, 201 |
| MspR9I | CCNGG | 1 cut(s) | 457 |
| MunI | CAATTG | 2 cut(s) | 18, 300 |
| MvaI | CCWGG | 1 cut(s) | 457 |
| NarI | GGCGCC | 1 cut(s) | 239 |
| NdeI | CATATG | 1 cut(s) | 198 |
| NdeII | GATC | 2 cut(s) | 64, 244 |
| NlaIII | CATG | 3 cut(s) | 332, 347, 496 |
| NlaIV | GGNNCC | 1 cut(s) | 240 |
| NsiI | ATGCAT | 1 cut(s) | 180 |
| NspI | RCATGY | 1 cut(s) | 347 |
| PciSI | GCTCTTC | 1 cut(s) | 414 |
| PdmI | GAANNNNTTC | 1 cut(s) | 464 |
| PfeI | GAWTC | 1 cut(s) | 40 |
| PfoI | TCCNGGA | 1 cut(s) | 455 |
| PkrI | GCNGC | 1 cut(s) | 298 |
| PluTI | GGCGCC | 1 cut(s) | 242 |
| PshAI | GACNNNNGTC | 1 cut(s) | 12 |
| Psp6I | CCWGG | 1 cut(s) | 455 |
| PspGI | CCWGG | 1 cut(s) | 455 |
| PspN4I | GGNNCC | 1 cut(s) | 240 |
| PstI | CTGCAG | 1 cut(s) | 14 |
| PsuI | RGATCY | 1 cut(s) | 244 |
| RseI | CAYNNNNRTG | 2 cut(s) | 35, 201 |
| SapI | GCTCTTC | 1 cut(s) | 414 |
| SaqAI | TTAA | 1 cut(s) | 369 |
| SatI | GCNGC | 1 cut(s) | 297 |
| Sau3AI | GATC | 2 cut(s) | 64, 244 |
| ScrFI | CCNGG | 1 cut(s) | 457 |
| SfaNI | GCATC | 1 cut(s) | 193 |
| SfcI | CTRYAG | 1 cut(s) | 10 |
| SfoI | GGCGCC | 1 cut(s) | 240 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 35, 201 |
| Sse9I | AATT | 3 cut(s) | 18, 69, 300 |
| SspDI | GGCGCC | 1 cut(s) | 238 |
| SspMI | CTAG | 3 cut(s) | 132, 186, 248 |
| StyD4I | CCNGG | 1 cut(s) | 455 |
| TaaI | ACNGT | 1 cut(s) | 359 |
| TaiI | ACGT | 1 cut(s) | 100 |
| TaqI | TCGA | 1 cut(s) | 468 |
| TasI | AATT | 3 cut(s) | 18, 69, 300 |
| TfiI | GAWTC | 1 cut(s) | 40 |
| Tru1I | TTAA | 1 cut(s) | 369 |
| Tru9I | TTAA | 1 cut(s) | 369 |
| TseI | GCWGC | 1 cut(s) | 296 |
| TspDTI | ATGAA | 1 cut(s) | 53 |
| XapI | RAATTY | 1 cut(s) | 69 |
| XceI | RCATGY | 1 cut(s) | 347 |
| XmnI | GAANNNNTTC | 1 cut(s) | 464 |
| XspI | CTAG | 3 cut(s) | 132, 186, 248 |
| Zsp2I | ATGCAT | 1 cut(s) | 180 |