MD04G1099000.v1.1
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000071
Physical Location & Seq
Forward (+)
18408054 .. 18411041
2988 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1099000.v1.1.491

Sequence Viewer

Length: 2988 bp
ATGGCAACTGACTCTTCCAGCAAACACACCATGGCTCTCACTTCCGAGCAATATCACAACCTGCTTGCACTCCTTGATAAGAGTCAATCCAACTCCACATCTAATCATGTCGGTACCACCTCTACTATGAATAATTTGTCAGATAGAGTTTTTTGTGCTTCTGCTCTCAATAAGGGAAGAAACTGGATTTTCGATACAGGCGCCACAGACCACATGGTCTGCTCACCAAGCTTAATGACCACAAGTGTTCCGGTATACAATCGACAAGTGCACTTACCCAATCATGCACTTGCTGATGTCACACACATTGGCACAGTTCATCTCTCGAATGATCTCATCCTTCACAACGTGCTTTGTGTTCCATCATTTAAACTGAATTTAATTTCTGTCAGCAAACTCACTAATACCTCATCTTGCTTTGTCATCTTCACTGATAAAATGTGTCTACTTCAGGGCCAACGATCGGGGAAGATGATTGGGACGGGAACTGAACGAGAAGGACTCTACTACCTTGATAATGTGATGAAAACCAGGTGCAATTCAGCGTCAATTGTTGCCTCTTCTTCTTCTCAGCTTTGGCATCAACGCCTTGGCCATCTTTCCAACACGACCTTACGGCATCTCTCTACTTGCATTAAAAATTTGAATTTTTGTAGCATTGATGATTGTTTGATCTGTCCATTAGCCAAACAAACACGTGCTCCTTTTCCTTTGAGTTCTATCACCACAAAGGCTCCTTTTGAGTTAATTCATGTTGACATTTGGGGAGGTTACCATGTTCCCACTATCACAGGGGCACAATATTTCTTAACTATTGTGGATGATTTCTCAAGATGCACATGGGTTTATTTAATGAATCACAAATCAGATGCACGATCTTACCTCATCACTTTCATCCACCTTGTTGAAACACAATTTTCCACACGAGTCAAGGTTCTTCGTAGTGATAACGGTCCAGAATTTTTTATGACCCAATTTTTTTCAGATAAGGGGATTCTCCATCAAACAAGTTGTGTCTCTACTCCCCAACAAAACGGGGTAGGAGAGCGCAAACACCGCCACTTACTCAATGTTGCTAGAGCCTTACTTTTTCAATCTCATCTTCCTAAGAAATTTTGGGGTGACGCAATCCTCACTGCCACTTATCTCATAAATAGGACTCCCACTCCTCTTCTTCAAGGCAAATCACCCTATGAAATACTTCACCACAAACAGCCAGCGTATGCACATTTGCGAGTGTTTGGTTGCTTATGTTTTTCTTCCACCCACCACCATCGTCCCACAAAATTTGATGCAAGAGCCACACGGTGTCTTTTTCTTGGGTATCCATATGGCACAAAGGGATATCGAGTTTACGACCTTCACACGGGTAAAATCTTTATCTCCCGTGATGTTATTTTCCATGAACATGTGTTCCCTTTTTCATCTTCTGCTGCAGTTTCACCACTTGTGTTTCCACATACTCAAGATACTGCCTGTGATGATCCCATCATTCATCCGTCCATTCTACTGGAGCAGGATTCCGTCGATCCATCCTCTTCTTCGCCGGTGAGTCCTGTTTCGGTTTCATCTCCTTTTCTTTCCACATCACCACCTCGTCTTAAACGACCCACTCATGCACCTGGTTATCTTCAACAATATCACATCGACATCAACCTCCCTTCTCGGTCTGTCCCTTCATCTCACTCGGCGTTGACCAAAGTACCAGGTACTCCTTACCCTTTGTCTGATGTCCTCACATACGATCGTTTATCTGCTCGACATCGTGCTTTCACCACTTCCATTTCAGCTGCCATAGCACCCAGCTCATTCTCACAGGCTATACGCGACCCTCACTGGCGTTTAGCCATGGAAGAAGAACTGGCAGCCCTTGCTGCGAATAGGACATGGTCTCTGCACCCATTGCCCCCTGGCAAGAAGCCGGTTGGATGTAAGTGGGTTTACAAAATCAAATTTCGATCCGATGGATCCATTGAGAGATATAAAGCACGCTTGGTTGCCAAAGATTATAGCCAAATTGAAGGCTTAGACTATCGAGAGACCTTTGCTCCGGTTGCAAAATTGACTACAGTTCGTCTACTCCTTGCTGTTGCCTCGGCCCAACAATGGCACCTCCACCAGCTTGATGTTAATAACGCTTTCCTCCATGGTGATCTTGAAGAAGATGTCTACATGTCCTTGCCTCCTGGTTTCGGACGAAAGGGGGAGACACGAGTTTGTAAACTTCATAAATCTTTGTATGGGCTTAAGCAAGCCTCTCGTCAATGGTTTATCAAACTCTCTTGTGCTCTTAGAAAGGCCGGTTATCAACAATCCAAGGCAGATTATTCATTGTTCGTCCGAAACCATGATGGTAAATTCACTGCCCTCCTTGTCTATGTTGATGATATTATCTTGGCAGGAAATAACATACAAGCAATTGAAGATACCAAGTCTTTTCTTATGAAGCAATTCAAGTTGAAAGACCTTGGCCAATTAAAATATTTCTTGGGCATTGAAATTGCCCGCTCTAGCAAGGGGATCACCCTCTCACAGCGAAAATATGCCCTTGAAATACTTGAAGATGCAGGTCATCTTGGCGCAAAACCAGCCACCTCTCCTATGGAGCAAAACTTATCACTCAGAAAGGACGAAGGACAATGGGTTACTGATCCTTCCTCTTATCGACGACTTGTTGGGAGACTAATCTACCTGACAATCACGCGCCCTGATTTGGTGTATGCTGTCCACATTCTTAGTCAATTTATGGAGAAGCCTCGAACTCCACACTTGGAGGCAGCCCAGCGAGTCTTACGATACGTTAAGCACACACCAGGGCAAGGTATTTTCCTTTCTGCAAGAAGTCCAATTCAATTGAATGCCTTTTGTGATGCGGATTGGGCCAGATGTCGAGACACACGACGTTCTACCACCGGATACTGTGTATTTCTTGGAGAATCCTTGATCTCCTGGAAAAGCAAGAAACAAAATACTGTTGTCACGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

996

Amino Acids

111.8

Weight (kDa)

9.38

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 62 - 135 1.6e-13 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 167 - 231 2.2e-11 GAG-pre-integrase domain
rve PF00665 245 - 342 8.5e-12 Integrase core domain
SH3_retrovirus PF25597 416 - 477 4.7e-24 Retroviral polymerase SH3-like domain
RVT_2 PF07727 627 - 869 9e-76 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
Acc36I ACCTGC 2 cut(s) 69, 2558
Acc65I GGTACC 1 cut(s) 113
AccB1I GGYRCC 3 cut(s) 113, 200, 2109
AccBSI CCGCTC 1 cut(s) 2508
AccI GTMKAC 4 cut(s) 255, 445, 2077, 2169
AccII CGCG 2 cut(s) 1827, 2704
AciI CCGC 3 cut(s) 1057, 2506, 2873
AclWI GGATC 7 cut(s) 1478, 1523, 1953, 1962, 1975, 2528, 2645
AcoI YGGCCR 2 cut(s) 592, 2470
AcsI RAATTY 8 cut(s) 376, 640, 646, 959, 1112, 1286, 1952, 2357
AcuI CTGAAG 1 cut(s) 434
AcvI CACGTG 1 cut(s) 698
AcyI GRCGYC 1 cut(s) 201
AdeI CACNNNGTG 2 cut(s) 349, 1308
AfaI GTAC 3 cut(s) 115, 1704, 1711
AfiI CCNNNNNNNGG 6 cut(s) 463, 1366, 2106, 2192, 2713, 2819
AflII CTTAAG 1 cut(s) 2246
AflIII ACRYGT 3 cut(s) 695, 1408, 2172
AjnI CCWGG 7 cut(s) 530, 1621, 1705, 1909, 2185, 2812, 2948
AloI GAACNNNNNNTCC 2 cut(s) 1398, 1430
AluBI AGCT 5 cut(s) 231, 574, 1790, 1806, 2122
AluI AGCT 5 cut(s) 231, 574, 1790, 1806, 2122
Alw21I GWGCWC 3 cut(s) 273, 703, 2290
Alw26I GTCTC 6 cut(s) 1021, 1896, 2033, 2201, 2674, 2886
Alw44I GTGCAC 1 cut(s) 269
AlwI GGATC 7 cut(s) 1478, 1523, 1953, 1962, 1975, 2528, 2645
AoxI GGCC 6 cut(s) 454, 592, 2097, 2298, 2470, 2880
ApaLI GTGCAC 1 cut(s) 269
ApeKI GCWGC 5 cut(s) 1433, 1790, 1865, 1874, 2777
ApoI RAATTY 8 cut(s) 376, 640, 646, 959, 1112, 1286, 1952, 2357
Asp700I GAANNNNTTC 2 cut(s) 1200, 2450
Asp718I GGTACC 1 cut(s) 113
AspLEI GCGC 4 cut(s) 203, 1050, 2582, 2706
AspS9I GGNCC 4 cut(s) 454, 953, 2098, 2880
AvaII GGWCC 1 cut(s) 953
BaeGI GKGCMC 2 cut(s) 273, 799
BaeI ACNNNNGTAYC 2 cut(s) 1686, 1719
BalI TGGCCA 2 cut(s) 594, 2472
BamHI GGATCC 1 cut(s) 1967
BanI GGYRCC 3 cut(s) 113, 200, 2109
BauI CACGAG 2 cut(s) 924, 2211
BbrPI CACGTG 1 cut(s) 698
Bbv12I GWGCWC 3 cut(s) 273, 703, 2290
BbvI GCAGC 5 cut(s) 1420, 1777, 1861, 1877, 2789
BccI CCATC 8 cut(s) 370, 603, 1008, 1281, 1496, 1540, 1958, 2345
BceAI ACGGC 1 cut(s) 632
BcgI CGANNNNNNTGC 2 cut(s) 2240, 2274
BciT130I CCWGG 7 cut(s) 532, 1623, 1707, 1911, 2187, 2814, 2950
BciVI GTATCC 2 cut(s) 1335, 2909
BcoDI GTCTC 6 cut(s) 1021, 1896, 2033, 2201, 2674, 2886
BfaI CTAG 3 cut(s) 1077, 2511, 2986
BfmI CTRYAG 2 cut(s) 1434, 2067
BfoI RGCGCY 1 cut(s) 204
BfrI CTTAAG 1 cut(s) 2246
BfuAI ACCTGC 2 cut(s) 69, 2558
BfuI GTATCC 2 cut(s) 1335, 2909
BisI GCNGC 5 cut(s) 1434, 1791, 1866, 1875, 2778
BlsI GCNGC 5 cut(s) 1435, 1792, 1867, 1876, 2779
Bme1390I CCNGG 7 cut(s) 532, 1623, 1707, 1911, 2187, 2814, 2950
Bme18I GGWCC 1 cut(s) 953
BmgT120I GGNCC 4 cut(s) 454, 953, 2098, 2880
BmiI GGNNCC 5 cut(s) 115, 202, 735, 1969, 2111
BmrFI CCNGG 7 cut(s) 532, 1623, 1707, 1911, 2187, 2814, 2950
BmsI GCATC 7 cut(s) 589, 628, 824, 859, 1282, 2554, 2860
BplI GAGNNNNNCTC 2 cut(s) 486, 518
BpmI CTGGAG 1 cut(s) 1532
BpuEI CTTGAG 2 cut(s) 814, 1449
BsaAI YACGTR 1 cut(s) 698
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 2 cut(s) 1896, 2033
BsaJI CCNNGG 9 cut(s) 30, 589, 1848, 1909, 2094, 2146, 2316, 2467, 2813
BsaWI WCCGGW 3 cut(s) 250, 2050, 2912
BsaXI ACNNNNNCTCC 8 cut(s) 685, 715, 718, 748, 1150, 1180, 2032, 2062
Bsc4I CCNNNNNNNGG 6 cut(s) 463, 1366, 2106, 2192, 2713, 2819
Bse118I RCCGGY 3 cut(s) 1546, 1921, 2300
Bse1I ACTGG 4 cut(s) 188, 1515, 1841, 1866
Bse3DI GCAATG 1 cut(s) 1901
BseBI CCWGG 7 cut(s) 532, 1623, 1707, 1911, 2187, 2814, 2950
BseDI CCNNGG 9 cut(s) 30, 589, 1848, 1909, 2094, 2146, 2316, 2467, 2813
BseGI GGATG 6 cut(s) 336, 826, 894, 1495, 1532, 1934
BseLI CCNNNNNNNGG 6 cut(s) 463, 1366, 2106, 2192, 2713, 2819
BseMI GCAATG 1 cut(s) 1901
BseMII CTCAG 2 cut(s) 584, 2635
BseNI ACTGG 4 cut(s) 188, 1515, 1841, 1866
BseRI GAGGAG 1 cut(s) 1158
BseSI GKGCMC 2 cut(s) 273, 799
BseXI GCAGC 5 cut(s) 1420, 1777, 1861, 1877, 2789
BseYI CCCAGC 2 cut(s) 1802, 2781
BsgI GTGCAG 1 cut(s) 1880
Bsh1236I CGCG 2 cut(s) 1827, 2704
Bsh1285I CGRYCG 2 cut(s) 464, 1747
BshFI GGCC 6 cut(s) 456, 594, 2099, 2300, 2472, 2882
BshNI GGYRCC 3 cut(s) 113, 200, 2109
BsiEI CGRYCG 2 cut(s) 464, 1747
BsiHKAI GWGCWC 3 cut(s) 273, 703, 2290
BsiSI CCGG 6 cut(s) 251, 1547, 1922, 2051, 2301, 2913
BslFI GGGAC 3 cut(s) 493, 1263, 1658
BslI CCNNNNNNNGG 6 cut(s) 463, 1366, 2106, 2192, 2713, 2819
BsmAI GTCTC 6 cut(s) 1021, 1896, 2033, 2201, 2674, 2886
BsmFI GGGAC 3 cut(s) 493, 1263, 1658
BsmI GAATGC 1 cut(s) 2863
BsnI GGCC 6 cut(s) 456, 594, 2099, 2300, 2472, 2882
Bso31I GGTCTC 2 cut(s) 1896, 2033
Bsp1286I GDGCHC 4 cut(s) 273, 703, 799, 2290
Bsp19I CCATGG 3 cut(s) 30, 1848, 2146
BspACI CCGC 3 cut(s) 1057, 2506, 2873
BspANI GGCC 6 cut(s) 456, 594, 2099, 2300, 2472, 2882
BspCNI CTCAG 2 cut(s) 583, 2634
BspFNI CGCG 2 cut(s) 1827, 2704
BspLI GGNNCC 5 cut(s) 115, 202, 735, 1969, 2111
BspMAI CTGCAG 1 cut(s) 1438
BspMI ACCTGC 2 cut(s) 69, 2558
BspPI GGATC 7 cut(s) 1478, 1523, 1953, 1962, 1975, 2528, 2645
BspT107I GGYRCC 3 cut(s) 113, 200, 2109
BspTI CTTAAG 1 cut(s) 2246
BspTNI GGTCTC 2 cut(s) 1896, 2033
BsrBI CCGCTC 1 cut(s) 2508
BsrDI GCAATG 1 cut(s) 1901
BsrFI RCCGGY 3 cut(s) 1546, 1921, 2300
BsrI ACTGG 4 cut(s) 188, 1515, 1841, 1866
BssAI RCCGGY 3 cut(s) 1546, 1921, 2300
BssECI CCNNGG 9 cut(s) 30, 589, 1848, 1909, 2094, 2146, 2316, 2467, 2813
BssNAI GTATAC 1 cut(s) 256
BssNI GRCGYC 1 cut(s) 201
BssSI CACGAG 2 cut(s) 924, 2211
BssT1I CCWWGG 6 cut(s) 30, 589, 1848, 2146, 2316, 2467
Bst1107I GTATAC 1 cut(s) 256
Bst2BI CACGAG 2 cut(s) 924, 2211
Bst2UI CCWGG 7 cut(s) 532, 1623, 1707, 1911, 2187, 2814, 2950
Bst4CI ACNGT 6 cut(s) 316, 953, 1308, 2071, 2921, 2974
Bst6I CTCTTC 4 cut(s) 19, 565, 1176, 1543
BstACI GRCGYC 1 cut(s) 201
BstAFI CTTAAG 1 cut(s) 2246
BstAPI GCANNNNNTGC 2 cut(s) 1871, 1903
BstBAI YACGTR 1 cut(s) 698
BstC8I GCNNGC 5 cut(s) 66, 1218, 1990, 2253, 2506
BstDEI CTNAG 6 cut(s) 570, 1107, 2026, 2291, 2621, 2735
BstDSI CCRYGG 3 cut(s) 30, 1848, 2146
BstEII GGTNACC 1 cut(s) 770
BstF5I GGATG 6 cut(s) 336, 826, 894, 1495, 1532, 1934
BstFNI CGCG 2 cut(s) 1827, 2704
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 4 cut(s) 203, 1050, 2582, 2706
BstMAI GTCTC 6 cut(s) 1021, 1896, 2033, 2201, 2674, 2886
BstMCI CGRYCG 2 cut(s) 464, 1747
BstMWI GCNNNNNNNGC 9 cut(s) 228, 1056, 1796, 1871, 1874, 1903, 2054, 2588, 2879
BstNI CCWGG 7 cut(s) 532, 1623, 1707, 1911, 2187, 2814, 2950
BstNSI RCATGY 2 cut(s) 1412, 2176
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 7 cut(s) 530, 1621, 1705, 1909, 2185, 2812, 2948
BstSFI CTRYAG 2 cut(s) 1434, 2067
BstSLI GKGCMC 2 cut(s) 273, 799
BstUI CGCG 2 cut(s) 1827, 2704
BstV1I GCAGC 5 cut(s) 1420, 1777, 1861, 1877, 2789
BstX2I RGATCY 1 cut(s) 1967
BstXI CCANNNNNNTGG 1 cut(s) 1510
BstYI RGATCY 1 cut(s) 1967
BstZ17I GTATAC 1 cut(s) 256
BsuI GTATCC 2 cut(s) 1335, 2909
BsuRI GGCC 6 cut(s) 456, 594, 2099, 2300, 2472, 2882
BtgI CCRYGG 3 cut(s) 30, 1848, 2146
BtsCI GGATG 6 cut(s) 336, 826, 894, 1495, 1532, 1934
BtsI GCAGTG 2 cut(s) 1134, 2361
BtsIMutI CAGTG 4 cut(s) 429, 1134, 1834, 2361
BveI ACCTGC 2 cut(s) 69, 2558
Cac8I GCNNGC 5 cut(s) 66, 1218, 1990, 2253, 2506
CfoI GCGC 4 cut(s) 203, 1050, 2582, 2706
Cfr10I RCCGGY 3 cut(s) 1546, 1921, 2300
Cfr13I GGNCC 4 cut(s) 454, 953, 2098, 2880
CseI GACGC 2 cut(s) 534, 1133
CsiI ACCWGGT 3 cut(s) 530, 1621, 1705
Csp6I GTAC 3 cut(s) 114, 1703, 1710
CspCI CAANNNNNGTGG 2 cut(s) 1270, 1305
CviQI GTAC 3 cut(s) 114, 1703, 1710
DdeI CTNAG 6 cut(s) 570, 1107, 2026, 2291, 2621, 2735
DinI GGCGCC 1 cut(s) 202
DraI TTTAAA 1 cut(s) 370
DraIII CACNNNGTG 2 cut(s) 349, 1308
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 2 cut(s) 592, 2470
Eam1104I CTCTTC 4 cut(s) 19, 565, 1176, 1543
EarI CTCTTC 4 cut(s) 19, 565, 1176, 1543
Eco130I CCWWGG 6 cut(s) 30, 589, 1848, 2146, 2316, 2467
Eco31I GGTCTC 2 cut(s) 1896, 2033
Eco32I GATATC 1 cut(s) 1346
Eco47I GGWCC 1 cut(s) 953
Eco57I CTGAAG 1 cut(s) 434
Eco72I CACGTG 1 cut(s) 698
Eco91I GGTNACC 1 cut(s) 770
EcoO65I GGTNACC 1 cut(s) 770
EcoRII CCWGG 7 cut(s) 530, 1621, 1705, 1909, 2185, 2812, 2948
EcoRV GATATC 1 cut(s) 1346
EcoT14I CCWWGG 6 cut(s) 30, 589, 1848, 2146, 2316, 2467
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
ErhI CCWWGG 6 cut(s) 30, 589, 1848, 2146, 2316, 2467
FaqI GGGAC 3 cut(s) 493, 1263, 1658
FauI CCCGC 1 cut(s) 2513
FauNDI CATATG 1 cut(s) 1330
FblI GTMKAC 4 cut(s) 255, 445, 2077, 2169
Fnu4HI GCNGC 5 cut(s) 1434, 1791, 1866, 1875, 2778
FokI GGATG 6 cut(s) 323, 833, 881, 1482, 1519, 1941
Fsp4HI GCNGC 5 cut(s) 1434, 1791, 1866, 1875, 2778
FspBI CTAG 3 cut(s) 1077, 2511, 2986
GlaI GCGC 4 cut(s) 202, 1049, 2581, 2705
GluI GCNGC 5 cut(s) 1434, 1791, 1866, 1875, 2778
GsaI CCCAGC 2 cut(s) 1806, 2785
GsuI CTGGAG 1 cut(s) 1532
HaeII RGCGCY 1 cut(s) 204
HaeIII GGCC 6 cut(s) 456, 594, 2099, 2300, 2472, 2882
HapII CCGG 6 cut(s) 251, 1547, 1922, 2051, 2301, 2913
HgaI GACGC 2 cut(s) 534, 1133
HhaI GCGC 4 cut(s) 203, 1050, 2582, 2706
Hin1I GRCGYC 1 cut(s) 201
Hin6I GCGC 4 cut(s) 201, 1048, 2580, 2704
HinP1I GCGC 4 cut(s) 201, 1048, 2580, 2704
HincII GTYRAC 2 cut(s) 757, 1695
HindII GTYRAC 2 cut(s) 757, 1695
HindIII AAGCTT 1 cut(s) 229
HpaII CCGG 6 cut(s) 251, 1547, 1922, 2051, 2301, 2913
Hpy188I TCNGA 9 cut(s) 46, 142, 868, 985, 1729, 1963, 2195, 2342, 2624
Hpy188III TCNNGA 7 cut(s) 325, 831, 956, 1466, 2036, 2156, 2891
Hpy99I CGWCG 3 cut(s) 1529, 2670, 2904
HpyAV CCTTC 8 cut(s) 350, 491, 1370, 1671, 1686, 2015, 2627, 2664
HpyCH4III ACNGT 6 cut(s) 316, 953, 1308, 2071, 2921, 2974
HpyCH4IV ACGT 5 cut(s) 348, 697, 2799, 2902, 2981
HpyF10VI GCNNNNNNNGC 9 cut(s) 228, 1056, 1796, 1871, 1874, 1903, 2054, 2588, 2879
HpyF3I CTNAG 6 cut(s) 570, 1107, 2026, 2291, 2621, 2735
HpySE526I ACGT 5 cut(s) 348, 697, 2799, 2902, 2981
Hsp92I GRCGYC 1 cut(s) 201
HspAI GCGC 4 cut(s) 201, 1048, 2580, 2704
KasI GGCGCC 1 cut(s) 200
KpnI GGTACC 1 cut(s) 117
LmnI GCTCC 5 cut(s) 706, 739, 1513, 2053, 2605
Lsp1109I GCAGC 5 cut(s) 1420, 1777, 1861, 1877, 2789
LweI GCATC 7 cut(s) 589, 628, 824, 859, 1282, 2554, 2860
MabI ACCWGGT 3 cut(s) 530, 1621, 1705
MaeI CTAG 3 cut(s) 1077, 2511, 2986
MaeII ACGT 5 cut(s) 348, 697, 2799, 2902, 2981
MaeIII GTNAC 5 cut(s) 298, 770, 1121, 2644, 2977
MbiI CCGCTC 1 cut(s) 2508
MfeI CAATTG 3 cut(s) 549, 2418, 2852
MflI RGATCY 1 cut(s) 1967
MhlI GDGCHC 4 cut(s) 273, 703, 799, 2290
MlsI TGGCCA 2 cut(s) 594, 2472
MluNI TGGCCA 2 cut(s) 594, 2472
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 7 cut(s) 5, 91, 495, 936, 1153, 1561, 2796
MmeI TCCRAC 3 cut(s) 114, 627, 1906
Mox20I TGGCCA 2 cut(s) 594, 2472
MroXI GAANNNNTTC 2 cut(s) 1200, 2450
MscI TGGCCA 2 cut(s) 594, 2472
MslI CAYNNNNRTG 1 cut(s) 1407
Msp20I TGGCCA 2 cut(s) 594, 2472
MspA1I CMGCKG 1 cut(s) 1790
MspCI CTTAAG 1 cut(s) 2246
MspI CCGG 6 cut(s) 251, 1547, 1922, 2051, 2301, 2913
MspR9I CCNGG 7 cut(s) 532, 1623, 1707, 1911, 2187, 2814, 2950
MunI CAATTG 3 cut(s) 549, 2418, 2852
Mva1269I GAATGC 1 cut(s) 2863
MvaI CCWGG 7 cut(s) 532, 1623, 1707, 1911, 2187, 2814, 2950
MvnI CGCG 2 cut(s) 1827, 2704
MwoI GCNNNNNNNGC 9 cut(s) 228, 1056, 1796, 1871, 1874, 1903, 2054, 2588, 2879
NarI GGCGCC 1 cut(s) 201
NcoI CCATGG 3 cut(s) 30, 1848, 2146
NdeI CATATG 1 cut(s) 1330
NlaIV GGNNCC 5 cut(s) 115, 202, 735, 1969, 2111
NmeAIII GCCGAG 2 cut(s) 1667, 2075
NmuCI GTSAC 3 cut(s) 298, 1121, 2977
NspI RCATGY 2 cut(s) 1412, 2176
PciI ACATGT 2 cut(s) 1408, 2172
PcsI WCGNNNNNNNCGW 2 cut(s) 1603, 2896
PctI GAATGC 1 cut(s) 2863
PdmI GAANNNNTTC 2 cut(s) 1200, 2450
PfeI GAWTC 4 cut(s) 856, 994, 1520, 2936
PflFI GACNNNGTC 1 cut(s) 1888
PfoI TCCNGGA 1 cut(s) 2948
PkrI GCNGC 5 cut(s) 1435, 1792, 1867, 1876, 2779
Ple19I CGATCG 2 cut(s) 464, 1747
PleI GAGTC 7 cut(s) 5, 90, 495, 935, 1153, 1560, 2795
PluTI GGCGCC 1 cut(s) 204
PmaCI CACGTG 1 cut(s) 698
PmlI CACGTG 1 cut(s) 698
PpsI GAGTC 7 cut(s) 5, 90, 495, 935, 1153, 1560, 2795
Ppu21I YACGTR 1 cut(s) 698
PscI ACATGT 2 cut(s) 1408, 2172
Psp6I CCWGG 7 cut(s) 530, 1621, 1705, 1909, 2185, 2812, 2948
PspCI CACGTG 1 cut(s) 698
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 2 cut(s) 1802, 2781
PspGI CCWGG 7 cut(s) 530, 1621, 1705, 1909, 2185, 2812, 2948
PspN4I GGNNCC 5 cut(s) 115, 202, 735, 1969, 2111
PspPI GGNCC 4 cut(s) 454, 953, 2098, 2880
PstI CTGCAG 1 cut(s) 1438
PsuI RGATCY 1 cut(s) 1967
PsyI GACNNNGTC 1 cut(s) 1888
PvuI CGATCG 2 cut(s) 464, 1747
PvuII CAGCTG 1 cut(s) 1790
RsaI GTAC 3 cut(s) 115, 1704, 1711
RsaNI GTAC 3 cut(s) 114, 1703, 1710
RseI CAYNNNNRTG 1 cut(s) 1407
SatI GCNGC 5 cut(s) 1434, 1791, 1866, 1875, 2778
Sau96I GGNCC 4 cut(s) 454, 953, 2098, 2880
SchI GAGTC 7 cut(s) 5, 91, 495, 936, 1153, 1561, 2796
ScrFI CCNGG 7 cut(s) 532, 1623, 1707, 1911, 2187, 2814, 2950
SduI GDGCHC 4 cut(s) 273, 703, 799, 2290
SexAI ACCWGGT 3 cut(s) 530, 1621, 1705
SfaNI GCATC 7 cut(s) 589, 628, 824, 859, 1282, 2554, 2860
SfcI CTRYAG 2 cut(s) 1434, 2067
SfoI GGCGCC 1 cut(s) 202
SgrAI CRCCGGYG 1 cut(s) 1546
SinI GGWCC 1 cut(s) 953
SmiMI CAYNNNNRTG 1 cut(s) 1407
SmlI CTYRAG 3 cut(s) 829, 1464, 2246
SmoI CTYRAG 3 cut(s) 829, 1464, 2246
SsiI CCGC 3 cut(s) 1057, 2506, 2873
SspDI GGCGCC 1 cut(s) 200
SspI AATATT 2 cut(s) 803, 2483
SspMI CTAG 3 cut(s) 1077, 2511, 2986
StyD4I CCNGG 7 cut(s) 530, 1621, 1705, 1909, 2185, 2812, 2948
StyI CCWWGG 6 cut(s) 30, 589, 1848, 2146, 2316, 2467
TaaI ACNGT 6 cut(s) 316, 953, 1308, 2071, 2921, 2974
TaiI ACGT 5 cut(s) 351, 700, 2802, 2905, 2984
TaqII GACCGA 1 cut(s) 1656
TfiI GAWTC 4 cut(s) 856, 994, 1520, 2936
TscAI CASTG 4 cut(s) 436, 1141, 1841, 2368
TseFI GTSAC 3 cut(s) 298, 1121, 2977
TseI GCWGC 5 cut(s) 1433, 1790, 1865, 1874, 2777
Tsp45I GTSAC 3 cut(s) 298, 1121, 2977
TspGWI ACGGA 2 cut(s) 1488, 1513
TspRI CASTG 4 cut(s) 436, 1141, 1841, 2368
Tth111I GACNNNGTC 1 cut(s) 1888
Vha464I CTTAAG 1 cut(s) 2246
VneI GTGCAC 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 953
XapI RAATTY 8 cut(s) 376, 640, 646, 959, 1112, 1286, 1952, 2357
XceI RCATGY 2 cut(s) 1412, 2176
XcmI CCANNNNNNNNNTGG 2 cut(s) 211, 2599
XmiI GTMKAC 4 cut(s) 255, 445, 2077, 2169
XmnI GAANNNNTTC 2 cut(s) 1200, 2450
XspI CTAG 3 cut(s) 1077, 2511, 2986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.