Reverse transcriptase (RNA-dependent DNA polymerase)
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 25, 302 |
| AccI | GTMKAC | 1 cut(s) | 13 |
| AccIII | TCCGGA | 1 cut(s) | 213 |
| AciI | CCGC | 1 cut(s) | 542 |
| AclWI | GGATC | 1 cut(s) | 205 |
| AcoI | YGGCCR | 1 cut(s) | 134 |
| AcsI | RAATTY | 1 cut(s) | 475 |
| AcuI | CTGAAG | 1 cut(s) | 441 |
| AfaI | GTAC | 2 cut(s) | 59, 634 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 248 |
| AgsI | TTSAA | 1 cut(s) | 264 |
| AjnI | CCWGG | 1 cut(s) | 241 |
| AluBI | AGCT | 2 cut(s) | 90, 206 |
| AluI | AGCT | 2 cut(s) | 90, 206 |
| AlwI | GGATC | 1 cut(s) | 205 |
| Ama87I | CYCGRG | 1 cut(s) | 594 |
| Aor13HI | TCCGGA | 1 cut(s) | 213 |
| AoxI | GGCC | 1 cut(s) | 134 |
| ApeKI | GCWGC | 1 cut(s) | 539 |
| ApoI | RAATTY | 1 cut(s) | 475 |
| AseI | ATTAAT | 1 cut(s) | 113 |
| AsuHPI | GGTGA | 1 cut(s) | 560 |
| AsuNHI | GCTAGC | 1 cut(s) | 86 |
| AvaI | CYCGRG | 1 cut(s) | 594 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 74, 106 |
| BbvI | GCAGC | 1 cut(s) | 551 |
| BciT130I | CCWGG | 1 cut(s) | 243 |
| BfaI | CTAG | 2 cut(s) | 5, 87 |
| BfuAI | ACCTGC | 2 cut(s) | 25, 302 |
| BisI | GCNGC | 1 cut(s) | 540 |
| BlsI | GCNGC | 1 cut(s) | 541 |
| Bme1390I | CCNGG | 1 cut(s) | 243 |
| BmeT110I | CYCGRG | 1 cut(s) | 594 |
| BmrFI | CCNGG | 1 cut(s) | 243 |
| BmrI | ACTGGG | 2 cut(s) | 316, 557 |
| BmsI | GCATC | 1 cut(s) | 289 |
| BmtI | GCTAGC | 1 cut(s) | 90 |
| BmuI | ACTGGG | 2 cut(s) | 316, 557 |
| BsaJI | CCNNGG | 1 cut(s) | 513 |
| BsaWI | WCCGGW | 1 cut(s) | 213 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 248 |
| Bse1I | ACTGG | 3 cut(s) | 54, 311, 563 |
| Bse3DI | GCAATG | 1 cut(s) | 141 |
| BseAI | TCCGGA | 1 cut(s) | 213 |
| BseBI | CCWGG | 1 cut(s) | 243 |
| BseDI | CCNNGG | 1 cut(s) | 513 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 248 |
| BseMI | GCAATG | 1 cut(s) | 141 |
| BseMII | CTCAG | 2 cut(s) | 45, 212 |
| BseNI | ACTGG | 3 cut(s) | 54, 311, 563 |
| BseXI | GCAGC | 1 cut(s) | 551 |
| BsgI | GTGCAG | 1 cut(s) | 222 |
| BshFI | GGCC | 1 cut(s) | 136 |
| BsiHKCI | CYCGRG | 1 cut(s) | 594 |
| BsiSI | CCGG | 1 cut(s) | 214 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 248 |
| BsnI | GGCC | 1 cut(s) | 136 |
| BsoBI | CYCGRG | 1 cut(s) | 594 |
| Bsp13I | TCCGGA | 1 cut(s) | 213 |
| Bsp143I | GATC | 1 cut(s) | 210 |
| Bsp19I | CCATGG | 1 cut(s) | 513 |
| BspACI | CCGC | 1 cut(s) | 542 |
| BspANI | GGCC | 1 cut(s) | 136 |
| BspCNI | CTCAG | 2 cut(s) | 46, 213 |
| BspEI | TCCGGA | 1 cut(s) | 213 |
| BspMI | ACCTGC | 2 cut(s) | 25, 302 |
| BspOI | GCTAGC | 1 cut(s) | 90 |
| BspPI | GGATC | 1 cut(s) | 205 |
| BsrDI | GCAATG | 1 cut(s) | 141 |
| BsrI | ACTGG | 3 cut(s) | 54, 311, 563 |
| BssECI | CCNNGG | 1 cut(s) | 513 |
| BssMI | GATC | 1 cut(s) | 210 |
| BssT1I | CCWWGG | 1 cut(s) | 513 |
| Bst2UI | CCWGG | 1 cut(s) | 243 |
| Bst4CI | ACNGT | 2 cut(s) | 632, 644 |
| BstC8I | GCNNGC | 1 cut(s) | 88 |
| BstDEI | CTNAG | 2 cut(s) | 54, 221 |
| BstDSI | CCRYGG | 1 cut(s) | 513 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 246 |
| BstKTI | GATC | 1 cut(s) | 213 |
| BstMBI | GATC | 1 cut(s) | 210 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 308 |
| BstNI | CCWGG | 1 cut(s) | 243 |
| BstSCI | CCNGG | 1 cut(s) | 241 |
| BstV1I | GCAGC | 1 cut(s) | 551 |
| BsuRI | GGCC | 1 cut(s) | 136 |
| BtgI | CCRYGG | 1 cut(s) | 513 |
| BveI | ACCTGC | 2 cut(s) | 25, 302 |
| Cac8I | GCNNGC | 1 cut(s) | 88 |
| Csp6I | GTAC | 2 cut(s) | 58, 633 |
| CviAII | CATG | 4 cut(s) | 175, 446, 514, 580 |
| CviJI | RGCY | 8 cut(s) | 53, 86, 90, 136, 206, 252, 443, 512 |
| CviKI_1 | RGCY | 8 cut(s) | 53, 86, 90, 136, 206, 252, 443, 512 |
| CviQI | GTAC | 2 cut(s) | 58, 633 |
| DdeI | CTNAG | 2 cut(s) | 54, 221 |
| DpnI | GATC | 1 cut(s) | 212 |
| DpnII | GATC | 1 cut(s) | 210 |
| DraI | TTTAAA | 2 cut(s) | 231, 499 |
| EaeI | YGGCCR | 1 cut(s) | 134 |
| Eco130I | CCWWGG | 1 cut(s) | 513 |
| Eco57I | CTGAAG | 1 cut(s) | 441 |
| Eco88I | CYCGRG | 1 cut(s) | 594 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 246 |
| EcoRII | CCWGG | 1 cut(s) | 241 |
| EcoT14I | CCWWGG | 1 cut(s) | 513 |
| EcoT22I | ATGCAT | 1 cut(s) | 180 |
| ErhI | CCWWGG | 1 cut(s) | 513 |
| FaeI | CATG | 4 cut(s) | 178, 449, 517, 583 |
| FatI | CATG | 4 cut(s) | 174, 445, 513, 579 |
| FauNDI | CATATG | 2 cut(s) | 148, 198 |
| FblI | GTMKAC | 1 cut(s) | 13 |
| Fnu4HI | GCNGC | 1 cut(s) | 540 |
| Fsp4HI | GCNGC | 1 cut(s) | 540 |
| FspBI | CTAG | 2 cut(s) | 5, 87 |
| GluI | GCNGC | 1 cut(s) | 540 |
| HaeIII | GGCC | 1 cut(s) | 136 |
| HapII | CCGG | 1 cut(s) | 214 |
| Hin1II | CATG | 4 cut(s) | 178, 449, 517, 583 |
| HinfI | GANTC | 3 cut(s) | 40, 166, 217 |
| HpaII | CCGG | 1 cut(s) | 214 |
| HphI | GGTGA | 1 cut(s) | 560 |
| Hpy166II | GTNNAC | 2 cut(s) | 14, 568 |
| Hpy188I | TCNGA | 1 cut(s) | 222 |
| Hpy188III | TCNNGA | 5 cut(s) | 214, 278, 485, 594, 596 |
| Hpy8I | GTNNAC | 2 cut(s) | 14, 568 |
| HpyAV | CCTTC | 1 cut(s) | 76 |
| HpyCH4III | ACNGT | 2 cut(s) | 632, 644 |
| HpyCH4IV | ACGT | 1 cut(s) | 587 |
| HpyCH4V | TGCA | 5 cut(s) | 146, 152, 178, 239, 302 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 308 |
| HpyF3I | CTNAG | 2 cut(s) | 54, 221 |
| HpySE526I | ACGT | 1 cut(s) | 587 |
| Hsp92II | CATG | 4 cut(s) | 178, 449, 517, 583 |
| Kpn2I | TCCGGA | 1 cut(s) | 213 |
| Kzo9I | GATC | 1 cut(s) | 210 |
| Lsp1109I | GCAGC | 1 cut(s) | 551 |
| LweI | GCATC | 1 cut(s) | 289 |
| MaeI | CTAG | 2 cut(s) | 5, 87 |
| MaeII | ACGT | 1 cut(s) | 587 |
| MaeIII | GTNAC | 4 cut(s) | 319, 344, 373, 638 |
| MalI | GATC | 1 cut(s) | 212 |
| MboI | GATC | 1 cut(s) | 210 |
| MboII | GAAGA | 1 cut(s) | 602 |
| MluCI | AATT | 5 cut(s) | 114, 170, 458, 475, 625 |
| MlyI | GAGTC | 1 cut(s) | 175 |
| MnlI | CCTC | 3 cut(s) | 216, 428, 601 |
| Mph1103I | ATGCAT | 1 cut(s) | 180 |
| MroI | TCCGGA | 1 cut(s) | 213 |
| MseI | TTAA | 4 cut(s) | 63, 113, 230, 498 |
| MspA1I | CMGCKG | 1 cut(s) | 542 |
| MspI | CCGG | 1 cut(s) | 214 |
| MspR9I | CCNGG | 1 cut(s) | 243 |
| MvaI | CCWGG | 1 cut(s) | 243 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 308 |
| NcoI | CCATGG | 1 cut(s) | 513 |
| NdeI | CATATG | 2 cut(s) | 148, 198 |
| NdeII | GATC | 1 cut(s) | 210 |
| NheI | GCTAGC | 1 cut(s) | 86 |
| NlaIII | CATG | 4 cut(s) | 178, 449, 517, 583 |
| NmeAIII | GCCGAG | 1 cut(s) | 112 |
| NsiI | ATGCAT | 1 cut(s) | 180 |
| PaeR7I | CTCGAG | 1 cut(s) | 594 |
| PfeI | GAWTC | 2 cut(s) | 40, 217 |
| PkrI | GCNGC | 1 cut(s) | 541 |
| PleI | GAGTC | 1 cut(s) | 174 |
| PpsI | GAGTC | 1 cut(s) | 174 |
| PshBI | ATTAAT | 1 cut(s) | 113 |
| Psp6I | CCWGG | 1 cut(s) | 241 |
| PspGI | CCWGG | 1 cut(s) | 241 |
| RsaI | GTAC | 2 cut(s) | 59, 634 |
| RsaNI | GTAC | 2 cut(s) | 58, 633 |
| SaqAI | TTAA | 4 cut(s) | 63, 113, 230, 498 |
| SatI | GCNGC | 1 cut(s) | 540 |
| Sau3AI | GATC | 1 cut(s) | 210 |
| SchI | GAGTC | 1 cut(s) | 175 |
| ScrFI | CCNGG | 1 cut(s) | 243 |
| SfaNI | GCATC | 1 cut(s) | 289 |
| Sfr274I | CTCGAG | 1 cut(s) | 594 |
| SlaI | CTCGAG | 1 cut(s) | 594 |
| SmlI | CTYRAG | 1 cut(s) | 594 |
| SmoI | CTYRAG | 1 cut(s) | 594 |
| Sse9I | AATT | 5 cut(s) | 114, 170, 458, 475, 625 |
| SsiI | CCGC | 1 cut(s) | 542 |
| SspMI | CTAG | 2 cut(s) | 5, 87 |
| StyD4I | CCNGG | 1 cut(s) | 241 |
| StyI | CCWWGG | 1 cut(s) | 513 |
| TaaI | ACNGT | 2 cut(s) | 632, 644 |
| TaiI | ACGT | 1 cut(s) | 590 |
| TaqI | TCGA | 1 cut(s) | 595 |
| TasI | AATT | 5 cut(s) | 114, 170, 458, 475, 625 |
| TatI | WGTACW | 1 cut(s) | 632 |
| TfiI | GAWTC | 2 cut(s) | 40, 217 |
| Tru1I | TTAA | 4 cut(s) | 63, 113, 230, 498 |
| Tru9I | TTAA | 4 cut(s) | 63, 113, 230, 498 |
| TseI | GCWGC | 1 cut(s) | 539 |
| TspDTI | ATGAA | 1 cut(s) | 163 |
| VspI | ATTAAT | 1 cut(s) | 113 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 246 |
| XapI | RAATTY | 1 cut(s) | 475 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 101, 511 |
| XhoI | CTCGAG | 1 cut(s) | 594 |
| XmiI | GTMKAC | 1 cut(s) | 13 |
| XspI | CTAG | 2 cut(s) | 5, 87 |
| Zsp2I | ATGCAT | 1 cut(s) | 180 |