RchiOBHm_Chr3g0460011

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000071
Physical Location & Seq
Forward (+)
8256863 .. 8259690
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42655

Sequence Viewer

Length: 2784 bp
ATGACTGTCCACTCTGATGTTTGGGGACCAGCTAAATATTCTACTATGTCAGGTGCTAAATACTTTGTTACTTTTATTGATGAGTGCACCCGGATGACATGGGTAATGTTGATGAAGAATAAGTGGGAATTATGTCAGGCGTTTCAAGCCTTTCACAAGATGGTTTCTAGCCAGTACCAGAGAAAGATTCAGGTCCTTCAGTCAGATAATGGGGGTGAGTATACGAGTAATGCCATGGCAGAGTTTTGCCAGTCTCAAGGCATTCGCCATCAAACTTCTTGTGCTTACACTCCGCAGCAAAATGGTCTAGCAGAACGAAAGAATCGTCAGTTGTTGGAAGTTCTTCGTGCCTCTCTCTTTGGAATGAATGTGCCTCGTGAATATTGGGGTGAAGCGTTGAAGTCTGCAACATATCTCATTAACCGCACTCCTTCTAGTGTCATTAATTTTGAGACTCCTCAACAAAAGTTAGGTACATTGATCTCTGCTCCTACTTTACCTAATTTGGAACCTCGAGTCTTTGGGTGCGTAGCATATGTACACATTCCTAAACATCTACGGCACAAACTTGATCCATGTGCCAAGAGATGTCTCTTTGTTGGTTATGCGGAGTTTAAAAAGGGGTATCGGTGTTATGATCCTCAGACGAAGACTCTCCATGTCTCTTTGGATGTATCTTTTCATGAAAATGAGGCTTACTTTACAGGCAAAGCTCATGAGCCTTCTCTTCAGGGGGAGAAAAGGTATATTGAAGAGAATTCTCAGGATATTTTTTCTGAAATTGAAGAGGTGGAGGAAAGATTGGTACAGCAGATTCTTTTCAACCCTCCAAAGGAAGGCACTAACTTGTCACATTCCACTCCAGCTGCTCCTGCCAATCCAGAGGGTTTTGACCCACCAATTATTCTCAACCAGTCGGATAGGCTAATAGAACAAACTGAAGAAGAAGCTGAGTTGCCCCCTTCTACACCAATGCATGAAGATACTTCCCGGGAGACTCACCCACAGGTAAACCCTTGTCAAGAAGTAGATTTGCATAATCCCATTGAGTCTAATCATTCATCTACTCCTAGAAGACCTGTTGAACCTAGATATCCTCAAAGATCAAACAAGGGAATTCCAAGGAAACAGTATGAACCTGATCTTAGGACAAAAGCCAAATATCCAATAGCTAACCATGTGTCTACCCATAGGTTGTCCGAGTCATATGCACTAACTGTCAATCAATTATCTACTGTGGCTATTCCTAGAACTGTGCAGGAAGCATTAGATGATCCAAAGTGGAGGATAGCAATGAATGAAGAGATGGAAGCACTTCAGAAGAATGATACTTGGGAGATTGCACCGTTACCTCAAGGAAAGAAGACTATCGGATGTAGATGGATATATACAGTGAAACTTAAGGCAGATGGGAGCATAGACAGGTACAAAGCAAGACTTGTAGCAAAAGGCTATACCCAGAAGTATGGAGTTGACTATCAGGAGACATTTGCTCCAGTGGCGAAAATTAATACCATTAGAATACTTATGTCTTTGGCCGCCACATATGATTGGCCATTACAACAGTATGATGTGAAGAATGCGTTCCTACATGGAGATTTAGAAGAAGAAGTTTATATGGACATGCCGCCAGGAAGTCAATGCAAGTTATCAGAGGTTGGTAAGGTGTGTAGGTTAAGGAAGTCATTGTATGGCTTGAAGCAATCACCCAGAGCATGGTTTGGAAGATTCACAAAGGCTATGAAGGCATTTGGCTATCGGCAGAGTAATTCTGATCACACTCTCTTTATCAGGCACAAAGCTGGTAAGTTGACAGTGCTCATAGTCTATGTAGATGATATGGTGGTAACCGGAGATGATCCAGTAGAAAGGAAAGCATTACAGGATTATTTGGCTACTGAATTTGAGATGAAAGATCTTGGACAACTGAAGTATTTCTTGGGGATTGAGGTAGCTAGGTCAAAAGAAGGGATCTCACTATCCCAACGAAAATATGTCCTTGATTTATTGACAGAAACTGGAATGCTTGATTGTCAACCAGTTGAGACACCCATGGAGATGAATCACAGACTAGGTGAATGGAAAGATCAGGTTCCCACAAATAAGTCCAGGTATCAATGTCTTGTTGGTAAATTAATATATTTATCTCATACTAGACCAGATATTGCATACGCAGTTGGTGTCGTTAGTCAGTTTATGCATGCTCCCAGTGAAGACCACATGAATGCAGTTTATCGAATTTTGAGGTATCTAAAGGCTGCTCCAGGGAAAGGATTGTTATTTGCCAAGAAAGACAAGTTGATAGTTGAGGGGTATACTGATGCAGATTGGGCAGGTTCAAAGGTAGATAGAAGATCTACTTCGGGATACTTTACCTTTGTTGGAGGTAACCTCGTGACATGGCGAAGTAAGAAGCAGAAAGTGGTTGCAAGATCTAGTGCTGAAGCAGAGTTCAGAGGCATGGCACATGGAGTTTGTGAGCTGCTGTGGATTAGACATCTGTTGAAAGATTTGGGCTTCAAGAATAGGAAGACAATGGACTTACATTGCGATAACAAGGCTGCAATTGAAATAGCACATAATCCAGTTCAACATGACCGTACAAAGCATGTGGAAGTAGATCGACACTTCATTAAGGAAAATTTGGACCGGAAAATAATTTATTTTCCGTTTGTCCAATCAGAAGAGCAGCTAGCAGATATTCTAACCAAAGCAGTTTCAGGGAAAGTATTTAACAGCTCTCTTGACAAGTTGGGCATGATTGATATCTACGCTCCAACTTGA

Protein Analysis

927

Amino Acids

105.98

Weight (kDa)

8.56

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
rve PF00665 4 - 98 1.4e-15 Integrase core domain
SH3_retrovirus PF25597 176 - 234 4.6e-24 Retroviral polymerase SH3-like domain
RVT_2 PF07727 442 - 686 1.7e-73 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2324
AccB7I CCANNNNNTGG 1 cut(s) 1716
AccI GTMKAC 3 cut(s) 221, 1184, 2315
AciI CCGC 5 cut(s) 293, 424, 608, 1539, 1628
AclWI GGATC 5 cut(s) 566, 632, 1268, 1853, 1979
AcoI YGGCCR 2 cut(s) 1536, 1553
AcsI RAATTY 5 cut(s) 757, 1116, 1901, 2238, 2641
AcuI CTGAAG 6 cut(s) 182, 713, 960, 1301, 1949, 2463
AfaI GTAC 6 cut(s) 176, 475, 540, 807, 1427, 2602
AfiI CCNNNNNNNGG 4 cut(s) 832, 836, 1716, 2270
AflII CTTAAG 1 cut(s) 1400
AjnI CCWGG 3 cut(s) 1630, 2108, 2263
AjuI GAANNNNNNNTTGG 2 cut(s) 1922, 1954
Alw21I GWGCWC 2 cut(s) 89, 1821
Alw26I GTCTC 7 cut(s) 258, 446, 596, 667, 989, 1478, 2039
Alw44I GTGCAC 1 cut(s) 85
AlwI GGATC 5 cut(s) 566, 632, 1268, 1853, 1979
AlwNI CAGNNNCTG 1 cut(s) 2018
Ama87I CYCGRG 2 cut(s) 513, 990
AoxI GGCC 2 cut(s) 1536, 1553
ApaLI GTGCAC 1 cut(s) 85
ApeKI GCWGC 6 cut(s) 295, 866, 2258, 2482, 2561, 2689
ApoI RAATTY 5 cut(s) 757, 1116, 1901, 2238, 2641
ArsI GACNNNNNNTTYG 2 cut(s) 310, 342
AseI ATTAAT 3 cut(s) 444, 1509, 2135
Asp700I GAANNNNTTC 4 cut(s) 342, 1314, 1583, 1934
AspS9I GGNCC 3 cut(s) 26, 193, 2647
AsuC2I CCSGG 3 cut(s) 91, 991, 992
AsuHPI GGTGA 5 cut(s) 227, 401, 992, 1698, 2087
AsuNHI GCTAGC 1 cut(s) 2692
AvaI CYCGRG 2 cut(s) 513, 990
AvaII GGWCC 3 cut(s) 26, 193, 2647
BaeGI GKGCMC 1 cut(s) 89
BalI TGGCCA 1 cut(s) 1555
BauI CACGAG 2 cut(s) 375, 2393
BbsI GAAGAC 5 cut(s) 656, 1081, 1370, 2220, 2537
Bbv12I GWGCWC 2 cut(s) 89, 1821
BbvI GCAGC 6 cut(s) 307, 853, 2245, 2469, 2548, 2701
BccI CCATC 5 cut(s) 154, 276, 1300, 1374, 1403
BceAI ACGGC 1 cut(s) 575
BcgI CGANNNNNNTGC 2 cut(s) 1190, 1224
BciT130I CCWGG 3 cut(s) 1632, 2110, 2265
BciVI GTATCC 1 cut(s) 2360
BclI TGATCA 1 cut(s) 1774
BcnI CCSGG 3 cut(s) 91, 991, 992
BcoDI GTCTC 7 cut(s) 258, 446, 596, 667, 989, 1478, 2039
BfrI CTTAAG 1 cut(s) 1400
BfuAI ACCTGC 1 cut(s) 2324
BfuI GTATCC 1 cut(s) 2360
BglII AGATCT 3 cut(s) 1915, 2354, 2432
BisI GCNGC 8 cut(s) 296, 867, 1539, 1628, 2259, 2483, 2562, 2690
BlsI GCNGC 8 cut(s) 297, 868, 1540, 1629, 2260, 2484, 2563, 2691
Bme1390I CCNGG 6 cut(s) 91, 991, 992, 1632, 2110, 2265
Bme18I GGWCC 3 cut(s) 26, 193, 2647
BmeT110I CYCGRG 2 cut(s) 513, 990
BmgT120I GGNCC 3 cut(s) 26, 193, 2647
BmiI GGNNCC 3 cut(s) 27, 510, 2094
BmrFI CCNGG 6 cut(s) 91, 991, 992, 1632, 2110, 2265
BmrI ACTGGG 1 cut(s) 2202
BmsI GCATC 1 cut(s) 2311
BmtI GCTAGC 1 cut(s) 2696
BmuI ACTGGG 1 cut(s) 2202
BpiI GAAGAC 5 cut(s) 656, 1081, 1370, 2220, 2537
BplI GAGNNNNNCTC 2 cut(s) 2376, 2408
BpmI CTGGAG 3 cut(s) 846, 1479, 2247
BpuEI CTTGAG 2 cut(s) 240, 1338
BpuMI CCSGG 3 cut(s) 91, 991, 992
BsaBI GATNNNNATC 1 cut(s) 2765
BsaJI CCNNGG 5 cut(s) 234, 990, 1121, 2052, 2264
BsaWI WCCGGW 2 cut(s) 1850, 2649
BsaXI ACNNNNNCTCC 4 cut(s) 728, 758, 1477, 1507
Bsc4I CCNNNNNNNGG 4 cut(s) 832, 836, 1716, 2270
Bse1I ACTGG 9 cut(s) 172, 250, 913, 1496, 1862, 2023, 2039, 2208, 2585
Bse3DI GCAATG 2 cut(s) 1299, 2545
Bse8I GATNNNNATC 1 cut(s) 2765
BseBI CCWGG 3 cut(s) 1632, 2110, 2265
BseDI CCNNGG 5 cut(s) 234, 990, 1121, 2052, 2264
BseGI GGATG 3 cut(s) 99, 676, 1379
BseJI GATNNNNATC 1 cut(s) 2765
BseLI CCNNNNNNNGG 4 cut(s) 832, 836, 1716, 2270
BseMI GCAATG 2 cut(s) 1299, 2545
BseMII CTCAG 3 cut(s) 656, 776, 942
BseNI ACTGG 9 cut(s) 172, 250, 913, 1496, 1862, 2023, 2039, 2208, 2585
BseRI GAGGAG 1 cut(s) 447
BseSI GKGCMC 1 cut(s) 89
BseXI GCAGC 6 cut(s) 307, 853, 2245, 2469, 2548, 2701
BsgI GTGCAG 1 cut(s) 1277
BshFI GGCC 2 cut(s) 1538, 1555
BsiHKAI GWGCWC 2 cut(s) 89, 1821
BsiHKCI CYCGRG 2 cut(s) 513, 990
BsiSI CCGG 4 cut(s) 91, 991, 1851, 2650
BslFI GGGAC 1 cut(s) 39
BslI CCNNNNNNNGG 4 cut(s) 832, 836, 1716, 2270
BsmAI GTCTC 7 cut(s) 258, 446, 596, 667, 989, 1478, 2039
BsmFI GGGAC 1 cut(s) 39
BsmI GAATGC 4 cut(s) 261, 1585, 2028, 2230
BsnI GGCC 2 cut(s) 1538, 1555
BsoBI CYCGRG 2 cut(s) 513, 990
Bsp1286I GDGCHC 2 cut(s) 89, 1821
Bsp1407I TGTACA 1 cut(s) 538
Bsp19I CCATGG 2 cut(s) 234, 2052
BspACI CCGC 5 cut(s) 293, 424, 608, 1539, 1628
BspANI GGCC 2 cut(s) 1538, 1555
BspCNI CTCAG 3 cut(s) 655, 775, 943
BspHI TCATGA 2 cut(s) 682, 715
BspLI GGNNCC 3 cut(s) 27, 510, 2094
BspMI ACCTGC 1 cut(s) 2324
BspOI GCTAGC 1 cut(s) 2696
BspPI GGATC 5 cut(s) 566, 632, 1268, 1853, 1979
BspQI GCTCTTC 1 cut(s) 2679
BspTI CTTAAG 1 cut(s) 1400
BsrDI GCAATG 2 cut(s) 1299, 2545
BsrGI TGTACA 1 cut(s) 538
BsrI ACTGG 9 cut(s) 172, 250, 913, 1496, 1862, 2023, 2039, 2208, 2585
BssECI CCNNGG 5 cut(s) 234, 990, 1121, 2052, 2264
BssNAI GTATAC 2 cut(s) 222, 2316
BssSI CACGAG 2 cut(s) 375, 2393
BssT1I CCWWGG 3 cut(s) 234, 1121, 2052
Bst1107I GTATAC 2 cut(s) 222, 2316
Bst2BI CACGAG 2 cut(s) 375, 2393
Bst2UI CCWGG 3 cut(s) 1632, 2110, 2265
Bst6I CTCTTC 5 cut(s) 732, 747, 780, 1296, 2679
BstAFI CTTAAG 1 cut(s) 1400
BstAUI TGTACA 1 cut(s) 538
BstC8I GCNNGC 2 cut(s) 2202, 2694
BstDEI CTNAG 4 cut(s) 642, 762, 951, 1145
BstDSI CCRYGG 2 cut(s) 234, 2052
BstEII GGTNACC 2 cut(s) 1846, 2387
BstF5I GGATG 3 cut(s) 99, 676, 1379
BstMAI GTCTC 7 cut(s) 258, 446, 596, 667, 989, 1478, 2039
BstMWI GCNNNNNNNGC 5 cut(s) 146, 872, 1499, 1745, 2330
BstNI CCWGG 3 cut(s) 1632, 2110, 2265
BstNSI RCATGY 3 cut(s) 1627, 2204, 2612
BstPI GGTNACC 2 cut(s) 1846, 2387
BstSCI CCNGG 6 cut(s) 89, 989, 990, 1630, 2108, 2263
BstSLI GKGCMC 1 cut(s) 89
BstV1I GCAGC 6 cut(s) 307, 853, 2245, 2469, 2548, 2701
BstV2I GAAGAC 5 cut(s) 656, 1081, 1370, 2220, 2537
BstX2I RGATCY 4 cut(s) 1915, 1971, 2354, 2432
BstXI CCANNNNNNTGG 1 cut(s) 1466
BstYI RGATCY 4 cut(s) 1915, 1971, 2354, 2432
BstZ17I GTATAC 2 cut(s) 222, 2316
BsuI GTATCC 1 cut(s) 2360
BsuRI GGCC 2 cut(s) 1538, 1555
BtgI CCRYGG 2 cut(s) 234, 2052
BtsCI GGATG 3 cut(s) 99, 676, 1379
BtsIMutI CAGTG 4 cut(s) 1398, 1503, 1821, 2215
BveI ACCTGC 1 cut(s) 2324
Cac8I GCNNGC 2 cut(s) 2202, 2694
CaiI CAGNNNCTG 1 cut(s) 2018
CciI TCATGA 2 cut(s) 682, 715
Cfr13I GGNCC 3 cut(s) 26, 193, 2647
Cfr9I CCCGGG 1 cut(s) 990
Csp6I GTAC 6 cut(s) 175, 474, 539, 806, 1426, 2601
CspCI CAANNNNNGTGG 2 cut(s) 2592, 2627
CviQI GTAC 6 cut(s) 175, 474, 539, 806, 1426, 2601
DdeI CTNAG 4 cut(s) 642, 762, 951, 1145
DraI TTTAAA 1 cut(s) 616
EaeI YGGCCR 2 cut(s) 1536, 1553
Eam1104I CTCTTC 5 cut(s) 732, 747, 780, 1296, 2679
EarI CTCTTC 5 cut(s) 732, 747, 780, 1296, 2679
Eco130I CCWWGG 3 cut(s) 234, 1121, 2052
Eco32I GATATC 2 cut(s) 1094, 2767
Eco47I GGWCC 3 cut(s) 26, 193, 2647
Eco57I CTGAAG 6 cut(s) 182, 713, 960, 1301, 1949, 2463
Eco88I CYCGRG 2 cut(s) 513, 990
Eco91I GGTNACC 2 cut(s) 1846, 2387
EcoO109I RGGNCCY 1 cut(s) 193
EcoO65I GGTNACC 2 cut(s) 1846, 2387
EcoRI GAATTC 2 cut(s) 757, 1116
EcoRII CCWGG 3 cut(s) 1630, 2108, 2263
EcoRV GATATC 2 cut(s) 1094, 2767
EcoT14I CCWWGG 3 cut(s) 234, 1121, 2052
EcoT22I ATGCAT 2 cut(s) 978, 2202
ErhI CCWWGG 3 cut(s) 234, 1121, 2052
FalI AAGNNNNNCTT 6 cut(s) 1422, 1454, 1922, 1954, 2344, 2376
FaqI GGGAC 1 cut(s) 39
FauNDI CATATG 3 cut(s) 535, 1207, 1546
FbaI TGATCA 1 cut(s) 1774
FblI GTMKAC 3 cut(s) 221, 1184, 2315
Fnu4HI GCNGC 8 cut(s) 296, 867, 1539, 1628, 2259, 2483, 2562, 2690
FokI GGATG 3 cut(s) 106, 683, 1386
Fsp4HI GCNGC 8 cut(s) 296, 867, 1539, 1628, 2259, 2483, 2562, 2690
GluI GCNGC 8 cut(s) 296, 867, 1539, 1628, 2259, 2483, 2562, 2690
GsuI CTGGAG 3 cut(s) 846, 1479, 2247
HaeIII GGCC 2 cut(s) 1538, 1555
HapII CCGG 4 cut(s) 91, 991, 1851, 2650
HincII GTYRAC 3 cut(s) 1474, 1812, 2036
HindII GTYRAC 3 cut(s) 1474, 1812, 2036
HpaII CCGG 4 cut(s) 91, 991, 1851, 2650
HphI GGTGA 5 cut(s) 227, 401, 992, 1698, 2087
HpyAV CCTTC 7 cut(s) 206, 441, 732, 830, 972, 1738, 1961
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 872, 1499, 1745, 2330
HpyF3I CTNAG 4 cut(s) 642, 762, 951, 1145
Ksp22I TGATCA 1 cut(s) 1774
LguI GCTCTTC 1 cut(s) 2679
LmnI GCTCC 7 cut(s) 493, 874, 1413, 1498, 2209, 2266, 2779
Lsp1109I GCAGC 6 cut(s) 307, 853, 2245, 2469, 2548, 2701
LweI GCATC 1 cut(s) 2311
MaeIII GTNAC 6 cut(s) 67, 849, 1347, 1846, 2387, 2395
MfeI CAATTG 1 cut(s) 2565
MflI RGATCY 4 cut(s) 1915, 1971, 2354, 2432
MhlI GDGCHC 2 cut(s) 89, 1821
MlsI TGGCCA 1 cut(s) 1555
MluNI TGGCCA 1 cut(s) 1555
MlyI GAGTC 6 cut(s) 448, 525, 646, 991, 1058, 1211
MmeI TCCRAC 3 cut(s) 315, 897, 2362
Mox20I TGGCCA 1 cut(s) 1555
Mph1103I ATGCAT 2 cut(s) 978, 2202
MroXI GAANNNNTTC 4 cut(s) 342, 1314, 1583, 1934
MscI TGGCCA 1 cut(s) 1555
MseI TTAA 9 cut(s) 420, 444, 615, 1401, 1509, 1676, 2135, 2634, 2733
MslI CAYNNNNRTG 5 cut(s) 15, 92, 687, 2057, 2223
Msp20I TGGCCA 1 cut(s) 1555
MspA1I CMGCKG 1 cut(s) 866
MspCI CTTAAG 1 cut(s) 1400
MspI CCGG 4 cut(s) 91, 991, 1851, 2650
MspR9I CCNGG 6 cut(s) 91, 991, 992, 1632, 2110, 2265
MunI CAATTG 1 cut(s) 2565
Mva1269I GAATGC 4 cut(s) 261, 1585, 2028, 2230
MvaI CCWGG 3 cut(s) 1632, 2110, 2265
MwoI GCNNNNNNNGC 5 cut(s) 146, 872, 1499, 1745, 2330
NciI CCSGG 3 cut(s) 91, 991, 992
NcoI CCATGG 2 cut(s) 234, 2052
NdeI CATATG 3 cut(s) 535, 1207, 1546
NheI GCTAGC 1 cut(s) 2692
NlaIV GGNNCC 3 cut(s) 27, 510, 2094
NmuCI GTSAC 2 cut(s) 849, 2395
NsiI ATGCAT 2 cut(s) 978, 2202
NspI RCATGY 3 cut(s) 1627, 2204, 2612
PaeI GCATGC 1 cut(s) 2204
PaeR7I CTCGAG 1 cut(s) 513
PagI TCATGA 2 cut(s) 682, 715
PciSI GCTCTTC 1 cut(s) 2679
PcsI WCGNNNNNNNCGW 1 cut(s) 322
PctI GAATGC 4 cut(s) 261, 1585, 2028, 2230
PdmI GAANNNNTTC 4 cut(s) 342, 1314, 1583, 1934
PfeI GAWTC 5 cut(s) 187, 322, 814, 1728, 2062
PflMI CCANNNNNTGG 1 cut(s) 1716
PkrI GCNGC 8 cut(s) 297, 868, 1540, 1629, 2260, 2484, 2563, 2691
PleI GAGTC 6 cut(s) 448, 524, 646, 991, 1057, 1210
PpsI GAGTC 6 cut(s) 448, 524, 646, 991, 1057, 1210
PpuMI RGGWCCY 1 cut(s) 193
PshBI ATTAAT 3 cut(s) 444, 1509, 2135
Psp5II RGGWCCY 1 cut(s) 193
Psp6I CCWGG 3 cut(s) 1630, 2108, 2263
PspEI GGTNACC 2 cut(s) 1846, 2387
PspGI CCWGG 3 cut(s) 1630, 2108, 2263
PspN4I GGNNCC 3 cut(s) 27, 510, 2094
PspPI GGNCC 3 cut(s) 26, 193, 2647
PspPPI RGGWCCY 1 cut(s) 193
PspXI VCTCGAGB 1 cut(s) 513
PstNI CAGNNNCTG 1 cut(s) 2018
PsuI RGATCY 4 cut(s) 1915, 1971, 2354, 2432
PvuII CAGCTG 1 cut(s) 866
RsaI GTAC 6 cut(s) 176, 475, 540, 807, 1427, 2602
RsaNI GTAC 6 cut(s) 175, 474, 539, 806, 1426, 2601
RseI CAYNNNNRTG 5 cut(s) 15, 92, 687, 2057, 2223
SapI GCTCTTC 1 cut(s) 2679
SaqAI TTAA 9 cut(s) 420, 444, 615, 1401, 1509, 1676, 2135, 2634, 2733
SatI GCNGC 8 cut(s) 296, 867, 1539, 1628, 2259, 2483, 2562, 2690
Sau96I GGNCC 3 cut(s) 26, 193, 2647
SchI GAGTC 6 cut(s) 448, 525, 646, 991, 1058, 1211
ScrFI CCNGG 6 cut(s) 91, 991, 992, 1632, 2110, 2265
SduI GDGCHC 2 cut(s) 89, 1821
SfaNI GCATC 1 cut(s) 2311
Sfr274I CTCGAG 1 cut(s) 513
SinI GGWCC 3 cut(s) 26, 193, 2647
SlaI CTCGAG 1 cut(s) 513
SmaI CCCGGG 1 cut(s) 992
SmiMI CAYNNNNRTG 5 cut(s) 15, 92, 687, 2057, 2223
SmlI CTYRAG 4 cut(s) 255, 513, 1353, 1400
SmoI CTYRAG 4 cut(s) 255, 513, 1353, 1400
SphI GCATGC 1 cut(s) 2204
SsiI CCGC 5 cut(s) 293, 424, 608, 1539, 1628
SspI AATATT 2 cut(s) 38, 383
StyD4I CCNGG 6 cut(s) 89, 989, 990, 1630, 2108, 2263
StyI CCWWGG 3 cut(s) 234, 1121, 2052
TaqI TCGA 3 cut(s) 514, 2236, 2623
TatI WGTACW 1 cut(s) 538
TauI GCSGC 2 cut(s) 1541, 1630
TfiI GAWTC 5 cut(s) 187, 322, 814, 1728, 2062
Tru1I TTAA 9 cut(s) 420, 444, 615, 1401, 1509, 1676, 2135, 2634, 2733
Tru9I TTAA 9 cut(s) 420, 444, 615, 1401, 1509, 1676, 2135, 2634, 2733
TscAI CASTG 4 cut(s) 1398, 1503, 1821, 2215
TseFI GTSAC 2 cut(s) 849, 2395
TseI GCWGC 6 cut(s) 295, 866, 2258, 2482, 2561, 2689
Tsp45I GTSAC 2 cut(s) 849, 2395
TspGWI ACGGA 1 cut(s) 2658
TspMI CCCGGG 1 cut(s) 990
TspRI CASTG 4 cut(s) 1398, 1503, 1821, 2215
Van91I CCANNNNNTGG 1 cut(s) 1716
Vha464I CTTAAG 1 cut(s) 1400
VneI GTGCAC 1 cut(s) 85
VpaK11BI GGWCC 3 cut(s) 26, 193, 2647
VspI ATTAAT 3 cut(s) 444, 1509, 2135
XapI RAATTY 5 cut(s) 757, 1116, 1901, 2238, 2641
XceI RCATGY 3 cut(s) 1627, 2204, 2612
XhoI CTCGAG 1 cut(s) 513
XmaI CCCGGG 1 cut(s) 990
XmiI GTMKAC 3 cut(s) 221, 1184, 2315
XmnI GAANNNNTTC 4 cut(s) 342, 1314, 1583, 1934
Zsp2I ATGCAT 2 cut(s) 978, 2202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.