FvH4_2g24232

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000071
Physical Location & Seq
Reverse (-)
19800277 .. 19803617
3341 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24232.t1

Sequence Viewer

Length: 2907 bp
ATGCAGTCTTTGGGTCAAGCTAATCAGGTCAATAGAGCTGAGAGTGCTAAGAAAGATGTGTGGCTTTGGCACCGCAGGTTGGGTCATCTAAACTTCGGTTATCTTAAGAAACTGCAACCGCAATTATTTTCAGATGTCGACTATTCAGATTTTCATTGCGACATATGTGAACTAGCCAAGAGCCACCGTATTTCATATCCACCAAGCCTAAATAAAAGTCCCGTTCCATTTATGAAGATTCACTCTGACGTGTGGGGTCCTGCAGAAATTCCTTCTCTCTCTGGTGCTCGGTATTTCGTGACGTTTATTGATGATTGTACCCATACGACTTGGGTGTCTCTATTGAATAATAAGAGCGATGTTTATTCTAGATTCAAAGAATTTCACAAGATGGTGTCCACACAGTATCAACAGGTTGTTCGAGTTTTCCAGTCTGACAATGGCGGAGAATATATTAATGGTCCTATGCAAGAGTTTATGAAGTTACATGGAATTCAATATCAGACTTCAAATACTTATACTTCTCAGCAAAATGGATTGGCAGAGAGAAAAAATCGGCAGTTGCTGGAAGTTGTCCGTGCCTCTTTGTTTGGCATGAATGTACCTGTAGAATATTGGGGAGAAGCTGTGACATCTGCTGCCTACCTCATTAATCGTATCCCTTCTCGGGTTATTGAATGTCAAAACCCTCTACAACAGCTTCAAAAACTTCTCTCCATTCTATCATTGCCTAACTTGGAGCCTCGTGTGTTTGGGTGCGTAGTATATGTTCATATTCCCAAGCAACAACGAAGCAAACTTGATCCTCGTGCTAAACGGTGTATCTTTCTTAGGTATGCCGAGTTTCAAAAAGGCTATCGATGTTATGATCCTCTCACTGATACCATTCACGTGTCCCTTGATGTTTCTTTTCGTGAATCTGAGCCTTACTATTCAGGGGGAGTTTCGACATCTTCCCTTCAAGGGGAGAGCGGTTGTGAAGGTGGAGGGAATTCTCGACAATTATTTGAATTCGACGAGTTTGAAGAGGTGGAAGCATTGGAGTTGAGATTCGGAGTTAATGGAGCCACAAATGATAAAGCAACAGAAAAGGAGACAACAACTCCTGTCCCGTTCAGTATTGATGCAGGCCACCCACGTGAAGCTCAGCCAAGCCGAGTGGAGACTGCAGCCGAGCAGAGCCGAACTGAAGGGGACCATGTGCGCGTCAGAGAGCCAAGCCGAGTGGAGACTTCAGCCGAGCCGAGCCGAACTGAAGAGGACCACGTTCGTGTCAGAGGAGAGCAGCACAAGGTGCCGCGTGTCGCCCAGGAGACTGCTGCGCGTGACAGAACAGAAGACATGCTGAAACTGGGTTTGCCACATGTCACGTCGGGATTGGTTGACACAGCTGATGTGGACACGCGTGTTGACCTAAAAAATACTGCCGCGTCAGACTTTGAAGCAGGTGTCGTCAGTCAAGAAGAAACGCAGGCTGTGCAACATGGGCCACATGGTACTGGTAGATTGGACACCCAAGGTTCTTCTCTGTCAGACAGTTTTCCTTTCTCTACACCATCAATTCAAGAATCCGCCGTGAATGTCCCTGCTCAAGCTCATGTACGTTTACATTCAAATCAATTACGTGAGGTAGATAGTATTCTACCTAGAAAGTCACAGAGAGTTACTAAAGGAATTCCAAAGAAACAATATGAACCTGATATCAAAGCCAAAGCTAAGTATCCTGTAGCCAATTACATGTCTAACCATAGATTGTCCGAGTCACACGCCCTTGTCATTAATCAATTATCCAGTGTTTGTATTCCTAGTAGTTTACAGGATGCAATGACGGATCCTAAGTGGACTCAAGCGATGAACGAAGAACTAGAGGCATTACAGAAAAACTCGACTTGGGATATTGTGCCTAAACTAGCTGGAAAGAAGACCGTTGGATGCCGTTGGGTATTCACCGTAAAGTTGAAAGCAGATGGGAGTATTGACAGGTATAAAGCAAGGTTGGTTGCCAAGGGATACACCCAGCGGTATGGCATTGATTATGAAGAAACGTTTGCGCCTATAGCAAAGATCAACACTGTACGAATCTTGATTTCACTTGCAGCAACTAAAGATTGGTCTCTACGACAGTTTGATGTCAAGAATGCTTTCCTCAATGGAAACTTGGAGGAAGAGGTGTATATGGACATGCCACTAGGAGTCAAAAGTAAACCATGCGATGCCGGCAAGGTTTGGGATGACCCAAAAGAGATGGAGGCTCTACAAAAGTATCTTTCAAAGGAGTTTGAGATGAAGGACCTTGGACAGTTGAAGTATTTTCTTGGAATTGAAGTTGCAAGTGAAGAACATATGGATGCAGTGTACCGTATTCTAAAGTATCTTAAGATGGCACCTGGCAAAGGCTTGTTGTTTGAAAAGAAGGGTGAATTGGAAGTTGTAGGGTACACCGATGCTGATTGGGCTGGTGATAAGACAGACAGACGATTTACATCTGGGTATTTTACATTTGTTGGAGGAAACCTTGTAACTTGGCGTAGTAAGAAGCAGAAGGTTGTTGCCAGGTCAAGTGCTGAAGCAGAATTTCGAGGTATGGCACATGGAGTTTGTGAGATGTTATGGATTCGTAATGTGTTAAAAGAATTGGGTTTTAAACTAAAAAAACCTATCGACTTGCATTGTGATAACACGTCTGCAATTGAAATTGCTCATAATCCTGTTCAACATGACCGGACCAAGCATGTGGAGGTAGATCGGCATTTTATCAAGGAAAACTTGGACAGAAAGATAATTTGTTTCCCTTTTGTGTATACAGAAGACCAATTGGCAAATGTTCTTACGAAAGGAGTGTCAAGGAAAGTGTTTGACGACTCAGTTGGCAAGTTGGGCATGATTGATATCTATGCACCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC RVT_2 TIR

Protein Analysis

969

Amino Acids

109.55

Weight (kDa)

6.99

Isoelectric Point (pI)

35.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 9 - 61 8.5e-15 GAG-pre-integrase domain
rve PF00665 79 - 174 3.2e-09 Integrase core domain
SH3_retrovirus PF25597 253 - 310 9.1e-24 Retroviral polymerase SH3-like domain
RVT_2 PF07727 628 - 744 1.2e-31 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1436
AasI GACNNNNNNGTC 1 cut(s) 334
Acc36I ACCTGC 2 cut(s) 66, 1436
AccB1I GGYRCC 3 cut(s) 69, 1294, 2383
AccBSI CCGCTC 1 cut(s) 972
AccI GTMKAC 2 cut(s) 138, 2801
AccII CGCG 5 cut(s) 1206, 1300, 1324, 1405, 1430
AciI CCGC 8 cut(s) 73, 119, 444, 972, 1298, 1428, 1572, 2020
AclI AACGTT 1 cut(s) 2045
AclWI GGATC 4 cut(s) 797, 863, 1826, 1839
AcsI RAATTY 7 cut(s) 267, 380, 492, 991, 1010, 1674, 2573
AcuI CTGAAG 4 cut(s) 1209, 1218, 1275, 2586
AcvI CACGTG 2 cut(s) 892, 1139
AdeI CACNNNGTG 1 cut(s) 1294
AfaI GTAC 7 cut(s) 319, 603, 1498, 1602, 2076, 2357, 2438
AfiI CCNNNNNNNGG 5 cut(s) 79, 667, 963, 964, 2393
AflII CTTAAG 2 cut(s) 104, 2375
AflIII ACRYGT 6 cut(s) 249, 891, 1363, 1403, 1737, 2679
AjiI CACGTC 3 cut(s) 250, 1371, 2682
AjnI CCWGG 3 cut(s) 1308, 2386, 2552
AjuI GAANNNNNNNTTGG 2 cut(s) 2405, 2437
AleI CACNNNNGTG 2 cut(s) 1137, 1269
AluBI AGCT 9 cut(s) 20, 38, 626, 700, 1145, 1391, 1595, 1715, 1913
AluI AGCT 9 cut(s) 20, 38, 626, 700, 1145, 1391, 1595, 1715, 1913
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 6 cut(s) 342, 1088, 1157, 1223, 1307, 2118
AlwI GGATC 4 cut(s) 797, 863, 1826, 1839
AlwNI CAGNNNCTG 1 cut(s) 565
Ama87I CYCGRG 1 cut(s) 666
AoxI GGCC 2 cut(s) 1129, 1487
ApeKI GCWGC 5 cut(s) 638, 1169, 1285, 1319, 2096
ApoI RAATTY 7 cut(s) 267, 380, 492, 991, 1010, 1674, 2573
ArsI GACNNNNNNTTYG 2 cut(s) 2825, 2857
AseI ATTAAT 3 cut(s) 456, 651, 1779
Asp700I GAANNNNTTC 1 cut(s) 2141
AspLEI GCGC 3 cut(s) 1206, 1324, 2053
AspS9I GGNCC 7 cut(s) 257, 461, 1195, 1261, 1487, 2290, 2724
AsuHPI GGTGA 3 cut(s) 1939, 2429, 2471
AvaI CYCGRG 1 cut(s) 666
AvaII GGWCC 6 cut(s) 257, 461, 1195, 1261, 2290, 2724
BaeI ACNNNNGTAYC 2 cut(s) 2246, 2279
BamHI GGATCC 1 cut(s) 1831
BanI GGYRCC 3 cut(s) 69, 1294, 2383
BauI CACGAG 2 cut(s) 744, 807
BbrPI CACGTG 2 cut(s) 892, 1139
BbsI GAAGAC 3 cut(s) 1344, 1928, 2814
Bbv12I GWGCWC 1 cut(s) 289
BbvI GCAGC 5 cut(s) 625, 1181, 1297, 1306, 2108
BccI CCATC 5 cut(s) 385, 1564, 1961, 2239, 2374
BceAI ACGGC 2 cut(s) 1559, 1920
BciT130I CCWGG 3 cut(s) 1310, 2388, 2554
BciVI GTATCC 3 cut(s) 668, 1731, 2003
BcoDI GTCTC 6 cut(s) 342, 1088, 1157, 1223, 1307, 2118
BfaI CTAG 7 cut(s) 173, 369, 1647, 1806, 1865, 1910, 2189
BfmI CTRYAG 5 cut(s) 261, 606, 1167, 1725, 2055
BfrI CTTAAG 2 cut(s) 104, 2375
BfuAI ACCTGC 2 cut(s) 66, 1436
BfuI GTATCC 3 cut(s) 668, 1731, 2003
BisI GCNGC 7 cut(s) 639, 1170, 1286, 1298, 1320, 1428, 2097
BlpI GCTNAGC 1 cut(s) 1146
BlsI GCNGC 7 cut(s) 640, 1171, 1287, 1299, 1321, 1429, 2098
Bme1390I CCNGG 3 cut(s) 1310, 2388, 2554
Bme18I GGWCC 6 cut(s) 257, 461, 1195, 1261, 2290, 2724
BmeT110I CYCGRG 1 cut(s) 666
BmgBI CACGTC 3 cut(s) 250, 1371, 2682
BmgT120I GGNCC 7 cut(s) 257, 461, 1195, 1261, 1487, 2290, 2724
BmiI GGNNCC 8 cut(s) 71, 258, 741, 1066, 1196, 1296, 1833, 2385
BmrFI CCNGG 3 cut(s) 1310, 2388, 2554
BmrI ACTGGG 1 cut(s) 1361
BmsI GCATC 6 cut(s) 1114, 1810, 1922, 2203, 2338, 2434
BmuI ACTGGG 1 cut(s) 1361
BpiI GAAGAC 3 cut(s) 1344, 1928, 2814
Bpu1102I GCTNAGC 1 cut(s) 1146
BpuEI CTTGAG 2 cut(s) 1575, 1830
Bsa29I ATCGAT 1 cut(s) 859
BsaAI YACGTR 3 cut(s) 892, 1139, 1625
BsaBI GATNNNNATC 2 cut(s) 2482, 2888
BsaI GGTCTC 1 cut(s) 2118
BsaJI CCNNGG 4 cut(s) 1308, 1516, 2004, 2293
BsaWI WCCGGW 1 cut(s) 2721
BsaXI ACNNNNNCTCC 4 cut(s) 612, 642, 1087, 1117
Bsc4I CCNNNNNNNGG 5 cut(s) 79, 667, 963, 964, 2393
Bse118I RCCGGY 1 cut(s) 2216
Bse1I ACTGG 4 cut(s) 430, 1356, 1504, 1791
Bse3DI GCAATG 3 cut(s) 154, 725, 1830
Bse8I GATNNNNATC 2 cut(s) 2482, 2888
BseBI CCWGG 3 cut(s) 1310, 2388, 2554
BseCI ATCGAT 1 cut(s) 859
BseDI CCNNGG 4 cut(s) 1308, 1516, 2004, 2293
BseGI GGATG 4 cut(s) 1825, 1937, 2236, 2353
BseJI GATNNNNATC 2 cut(s) 2482, 2888
BseLI CCNNNNNNNGG 5 cut(s) 79, 667, 963, 964, 2393
BseMI GCAATG 3 cut(s) 154, 725, 1830
BseMII CTCAG 5 cut(s) 30, 539, 912, 1160, 2877
BseNI ACTGG 4 cut(s) 430, 1356, 1504, 1791
BseRI GAGGAG 1 cut(s) 1293
BseXI GCAGC 5 cut(s) 625, 1181, 1297, 1306, 2108
BseYI CCCAGC 1 cut(s) 2016
Bsh1236I CGCG 5 cut(s) 1206, 1300, 1324, 1405, 1430
BshFI GGCC 2 cut(s) 1131, 1489
BshNI GGYRCC 3 cut(s) 69, 1294, 2383
BshVI ATCGAT 1 cut(s) 859
BsiHKAI GWGCWC 1 cut(s) 289
BsiHKCI CYCGRG 1 cut(s) 666
BsiSI CCGG 2 cut(s) 2217, 2722
BslFI GGGAC 5 cut(s) 204, 880, 1094, 1208, 1568
BslI CCNNNNNNNGG 5 cut(s) 79, 667, 963, 964, 2393
BsmAI GTCTC 6 cut(s) 342, 1088, 1157, 1223, 1307, 2118
BsmFI GGGAC 5 cut(s) 204, 880, 1094, 1208, 1568
BsmI GAATGC 1 cut(s) 2143
BsnI GGCC 2 cut(s) 1131, 1489
Bso31I GGTCTC 1 cut(s) 2118
BsoBI CYCGRG 1 cut(s) 666
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 5 cut(s) 802, 868, 1831, 2064, 2743
Bsp1720I GCTNAGC 1 cut(s) 1146
BspACI CCGC 8 cut(s) 73, 119, 444, 972, 1298, 1428, 1572, 2020
BspANI GGCC 2 cut(s) 1131, 1489
BspCNI CTCAG 5 cut(s) 31, 538, 913, 1159, 2876
BspDI ATCGAT 1 cut(s) 859
BspFNI CGCG 5 cut(s) 1206, 1300, 1324, 1405, 1430
BspLI GGNNCC 8 cut(s) 71, 258, 741, 1066, 1196, 1296, 1833, 2385
BspMAI CTGCAG 2 cut(s) 265, 1171
BspMI ACCTGC 2 cut(s) 66, 1436
BspPI GGATC 4 cut(s) 797, 863, 1826, 1839
BspT107I GGYRCC 3 cut(s) 69, 1294, 2383
BspTI CTTAAG 2 cut(s) 104, 2375
BspTNI GGTCTC 1 cut(s) 2118
BsrBI CCGCTC 1 cut(s) 972
BsrDI GCAATG 3 cut(s) 154, 725, 1830
BsrFI RCCGGY 1 cut(s) 2216
BsrI ACTGG 4 cut(s) 430, 1356, 1504, 1791
BssAI RCCGGY 1 cut(s) 2216
BssECI CCNNGG 4 cut(s) 1308, 1516, 2004, 2293
BssMI GATC 5 cut(s) 802, 868, 1831, 2064, 2743
BssNAI GTATAC 1 cut(s) 2802
BssSI CACGAG 2 cut(s) 744, 807
BssT1I CCWWGG 3 cut(s) 1516, 2004, 2293
Bst1107I GTATAC 1 cut(s) 2802
Bst2BI CACGAG 2 cut(s) 744, 807
Bst2UI CCWGG 3 cut(s) 1310, 2388, 2554
Bst6I CTCTTC 3 cut(s) 1020, 1251, 2160
BstAFI CTTAAG 2 cut(s) 104, 2375
BstAPI GCANNNNNTGC 2 cut(s) 1294, 1477
BstBAI YACGTR 3 cut(s) 892, 1139, 1625
BstC8I GCNNGC 3 cut(s) 1129, 1473, 2218
BstDEI CTNAG 9 cut(s) 39, 48, 525, 830, 921, 1146, 1716, 1836, 2863
BstENI CCTNNNNNAGG 1 cut(s) 2391
BstF5I GGATG 4 cut(s) 1825, 1937, 2236, 2353
BstFNI CGCG 5 cut(s) 1206, 1300, 1324, 1405, 1430
BstHHI GCGC 3 cut(s) 1206, 1324, 2053
BstKTI GATC 5 cut(s) 805, 871, 1834, 2067, 2746
BstMAI GTCTC 6 cut(s) 342, 1088, 1157, 1223, 1307, 2118
BstMBI GATC 5 cut(s) 802, 868, 1831, 2064, 2743
BstMWI GCNNNNNNNGC 8 cut(s) 44, 1294, 1477, 1486, 2057, 2217, 2453, 2877
BstNI CCWGG 3 cut(s) 1310, 2388, 2554
BstNSI RCATGY 5 cut(s) 1345, 1367, 1741, 2185, 2735
BstSCI CCNGG 3 cut(s) 1308, 2386, 2552
BstSFI CTRYAG 5 cut(s) 261, 606, 1167, 1725, 2055
BstUI CGCG 5 cut(s) 1206, 1300, 1324, 1405, 1430
BstV1I GCAGC 5 cut(s) 625, 1181, 1297, 1306, 2108
BstV2I GAAGAC 3 cut(s) 1344, 1928, 2814
BstX2I RGATCY 1 cut(s) 1831
BstXI CCANNNNNNTGG 2 cut(s) 2024, 2734
BstYI RGATCY 1 cut(s) 1831
BstZ17I GTATAC 1 cut(s) 2802
Bsu15I ATCGAT 1 cut(s) 859
BsuI GTATCC 3 cut(s) 668, 1731, 2003
BsuRI GGCC 2 cut(s) 1131, 1489
BsuTUI ATCGAT 1 cut(s) 859
BtgZI GCGATG 3 cut(s) 372, 1865, 2226
BtrI CACGTC 3 cut(s) 250, 1371, 2682
BtsCI GGATG 4 cut(s) 1825, 1937, 2236, 2353
BtsI GCAGTG 1 cut(s) 2358
BtsIMutI CAGTG 4 cut(s) 876, 1798, 2070, 2358
BveI ACCTGC 2 cut(s) 66, 1436
Cac8I GCNNGC 3 cut(s) 1129, 1473, 2218
CaiI CAGNNNCTG 1 cut(s) 565
CfoI GCGC 3 cut(s) 1206, 1324, 2053
Cfr10I RCCGGY 1 cut(s) 2216
Cfr13I GGNCC 7 cut(s) 257, 461, 1195, 1261, 1487, 2290, 2724
ClaI ATCGAT 1 cut(s) 859
CseI GACGC 2 cut(s) 1195, 1419
Csp6I GTAC 7 cut(s) 318, 602, 1497, 1601, 2075, 2356, 2437
CspCI CAANNNNNGTGG 4 cut(s) 1140, 1175, 1206, 1241
CviQI GTAC 7 cut(s) 318, 602, 1497, 1601, 2075, 2356, 2437
DdeI CTNAG 9 cut(s) 39, 48, 525, 830, 921, 1146, 1716, 1836, 2863
DpnI GATC 5 cut(s) 804, 870, 1833, 2066, 2745
DpnII GATC 5 cut(s) 802, 868, 1831, 2064, 2743
DraI TTTAAA 1 cut(s) 2644
DraIII CACNNNGTG 1 cut(s) 1294
DrdI GACNNNNNNGTC 1 cut(s) 334
DseDI GACNNNNNNGTC 1 cut(s) 334
Eam1104I CTCTTC 3 cut(s) 1020, 1251, 2160
EarI CTCTTC 3 cut(s) 1020, 1251, 2160
EciI GGCGGA 2 cut(s) 459, 1561
Eco130I CCWWGG 3 cut(s) 1516, 2004, 2293
Eco31I GGTCTC 1 cut(s) 2118
Eco32I GATATC 2 cut(s) 1702, 2890
Eco47I GGWCC 6 cut(s) 257, 461, 1195, 1261, 2290, 2724
Eco57I CTGAAG 4 cut(s) 1209, 1218, 1275, 2586
Eco72I CACGTG 2 cut(s) 892, 1139
Eco88I CYCGRG 1 cut(s) 666
EcoNI CCTNNNNNAGG 1 cut(s) 2391
EcoO109I RGGNCCY 2 cut(s) 257, 2290
EcoRI GAATTC 4 cut(s) 492, 991, 1010, 1674
EcoRII CCWGG 3 cut(s) 1308, 2386, 2552
EcoRV GATATC 2 cut(s) 1702, 2890
EcoT14I CCWWGG 3 cut(s) 1516, 2004, 2293
ErhI CCWWGG 3 cut(s) 1516, 2004, 2293
FalI AAGNNNNNCTT 2 cut(s) 2750, 2782
FaqI GGGAC 5 cut(s) 204, 880, 1094, 1208, 1568
FauNDI CATATG 2 cut(s) 164, 2343
FblI GTMKAC 2 cut(s) 138, 2801
Fnu4HI GCNGC 7 cut(s) 639, 1170, 1286, 1298, 1320, 1428, 2097
FokI GGATG 4 cut(s) 1832, 1944, 2243, 2360
Fsp4HI GCNGC 7 cut(s) 639, 1170, 1286, 1298, 1320, 1428, 2097
FspBI CTAG 7 cut(s) 173, 369, 1647, 1806, 1865, 1910, 2189
GlaI GCGC 3 cut(s) 1205, 1323, 2052
GluI GCNGC 7 cut(s) 639, 1170, 1286, 1298, 1320, 1428, 2097
GsaI CCCAGC 1 cut(s) 2020
HaeIII GGCC 2 cut(s) 1131, 1489
HapII CCGG 2 cut(s) 2217, 2722
HgaI GACGC 2 cut(s) 1195, 1419
HhaI GCGC 3 cut(s) 1206, 1324, 2053
Hin6I GCGC 3 cut(s) 1204, 1322, 2051
HinP1I GCGC 3 cut(s) 1204, 1322, 2051
HincII GTYRAC 3 cut(s) 139, 1384, 1411
HindII GTYRAC 3 cut(s) 139, 1384, 1411
HpaII CCGG 2 cut(s) 2217, 2722
HphI GGTGA 3 cut(s) 1939, 2429, 2471
Hpy188III TCNNGA 9 cut(s) 298, 369, 914, 996, 1374, 1460, 1565, 2083, 2134
Hpy99I CGWCG 2 cut(s) 1019, 1375
HpyAV CCTTC 8 cut(s) 282, 672, 968, 974, 1184, 2281, 2407, 2536
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 1294, 1477, 1486, 2057, 2217, 2453, 2877
HpyF3I CTNAG 9 cut(s) 39, 48, 525, 830, 921, 1146, 1716, 1836, 2863
HspAI GCGC 3 cut(s) 1204, 1322, 2051
KroI GCCGGC 1 cut(s) 2216
KroNI GCCGGC 1 cut(s) 2218
Kzo9I GATC 5 cut(s) 802, 868, 1831, 2064, 2743
LmnI GCTCC 2 cut(s) 739, 1064
Lsp1109I GCAGC 5 cut(s) 625, 1181, 1297, 1306, 2108
LweI GCATC 6 cut(s) 1114, 1810, 1922, 2203, 2338, 2434
MaeI CTAG 7 cut(s) 173, 369, 1647, 1806, 1865, 1910, 2189
MaeIII GTNAC 9 cut(s) 298, 483, 628, 1325, 1366, 1653, 1663, 1761, 2518
MalI GATC 5 cut(s) 804, 870, 1833, 2066, 2745
MbiI CCGCTC 1 cut(s) 972
MboI GATC 5 cut(s) 802, 868, 1831, 2064, 2743
MfeI CAATTG 2 cut(s) 2688, 2813
MflI RGATCY 1 cut(s) 1831
MhlI GDGCHC 1 cut(s) 289
MluI ACGCGT 1 cut(s) 1403
MlyI GAGTC 4 cut(s) 1769, 1837, 2202, 2855
MmeI TCCRAC 2 cut(s) 1909, 2485
MroNI GCCGGC 1 cut(s) 2216
MroXI GAANNNNTTC 1 cut(s) 2141
MseI TTAA 8 cut(s) 105, 456, 651, 1059, 1779, 2376, 2627, 2643
MslI CAYNNNNRTG 4 cut(s) 890, 1137, 1269, 2346
MspA1I CMGCKG 2 cut(s) 1391, 2020
MspCI CTTAAG 2 cut(s) 104, 2375
MspI CCGG 2 cut(s) 2217, 2722
MspR9I CCNGG 3 cut(s) 1310, 2388, 2554
MunI CAATTG 2 cut(s) 2688, 2813
Mva1269I GAATGC 1 cut(s) 2143
MvaI CCWGG 3 cut(s) 1310, 2388, 2554
MvnI CGCG 5 cut(s) 1206, 1300, 1324, 1405, 1430
MwoI GCNNNNNNNGC 8 cut(s) 44, 1294, 1477, 1486, 2057, 2217, 2453, 2877
NaeI GCCGGC 1 cut(s) 2218
NdeI CATATG 2 cut(s) 164, 2343
NdeII GATC 5 cut(s) 802, 868, 1831, 2064, 2743
NgoMIV GCCGGC 1 cut(s) 2216
NlaIV GGNNCC 8 cut(s) 71, 258, 741, 1066, 1196, 1296, 1833, 2385
NmeAIII GCCGAG 6 cut(s) 865, 1181, 1198, 1247, 1264, 1269
NmuCI GTSAC 6 cut(s) 298, 628, 1325, 1366, 1653, 1761
NspI RCATGY 5 cut(s) 1345, 1367, 1741, 2185, 2735
OliI CACNNNNGTG 2 cut(s) 1137, 1269
PaqCI CACCTGC 1 cut(s) 1436
PciI ACATGT 2 cut(s) 1363, 1737
PctI GAATGC 1 cut(s) 2143
PdiI GCCGGC 1 cut(s) 2218
PdmI GAANNNNTTC 1 cut(s) 2141
PfeI GAWTC 7 cut(s) 238, 372, 917, 1050, 1568, 2079, 2614
PkrI GCNGC 7 cut(s) 640, 1171, 1287, 1299, 1321, 1429, 2098
PleI GAGTC 4 cut(s) 1768, 1837, 2201, 2855
PmaCI CACGTG 2 cut(s) 892, 1139
PmlI CACGTG 2 cut(s) 892, 1139
PpsI GAGTC 4 cut(s) 1768, 1837, 2201, 2855
Ppu21I YACGTR 3 cut(s) 892, 1139, 1625
PpuMI RGGWCCY 2 cut(s) 257, 2290
PscI ACATGT 2 cut(s) 1363, 1737
PshBI ATTAAT 3 cut(s) 456, 651, 1779
Psp1406I AACGTT 1 cut(s) 2045
Psp5II RGGWCCY 2 cut(s) 257, 2290
Psp6I CCWGG 3 cut(s) 1308, 2386, 2552
PspCI CACGTG 2 cut(s) 892, 1139
PspFI CCCAGC 1 cut(s) 2016
PspGI CCWGG 3 cut(s) 1308, 2386, 2552
PspN4I GGNNCC 8 cut(s) 71, 258, 741, 1066, 1196, 1296, 1833, 2385
PspPI GGNCC 7 cut(s) 257, 461, 1195, 1261, 1487, 2290, 2724
PspPPI RGGWCCY 2 cut(s) 257, 2290
PsrI GAACNNNNNNTAC 2 cut(s) 753, 785
PstI CTGCAG 2 cut(s) 265, 1171
PstNI CAGNNNCTG 1 cut(s) 565
PsuI RGATCY 1 cut(s) 1831
PvuII CAGCTG 1 cut(s) 1391
RsaI GTAC 7 cut(s) 319, 603, 1498, 1602, 2076, 2357, 2438
RsaNI GTAC 7 cut(s) 318, 602, 1497, 1601, 2075, 2356, 2437
RseI CAYNNNNRTG 4 cut(s) 890, 1137, 1269, 2346
SalI GTCGAC 1 cut(s) 137
SaqAI TTAA 8 cut(s) 105, 456, 651, 1059, 1779, 2376, 2627, 2643
SatI GCNGC 7 cut(s) 639, 1170, 1286, 1298, 1320, 1428, 2097
Sau3AI GATC 5 cut(s) 802, 868, 1831, 2064, 2743
Sau96I GGNCC 7 cut(s) 257, 461, 1195, 1261, 1487, 2290, 2724
SchI GAGTC 4 cut(s) 1769, 1837, 2202, 2855
ScrFI CCNGG 3 cut(s) 1310, 2388, 2554
SduI GDGCHC 1 cut(s) 289
SfaNI GCATC 6 cut(s) 1114, 1810, 1922, 2203, 2338, 2434
SfcI CTRYAG 5 cut(s) 261, 606, 1167, 1725, 2055
SinI GGWCC 6 cut(s) 257, 461, 1195, 1261, 2290, 2724
SmiMI CAYNNNNRTG 4 cut(s) 890, 1137, 1269, 2346
SmlI CTYRAG 4 cut(s) 104, 1590, 1845, 2375
SmoI CTYRAG 4 cut(s) 104, 1590, 1845, 2375
SsiI CCGC 8 cut(s) 73, 119, 444, 972, 1298, 1428, 1572, 2020
SspI AATATT 1 cut(s) 614
SspMI CTAG 7 cut(s) 173, 369, 1647, 1806, 1865, 1910, 2189
StyD4I CCNGG 3 cut(s) 1308, 2386, 2552
StyI CCWWGG 3 cut(s) 1516, 2004, 2293
TaqI TCGA 9 cut(s) 138, 421, 859, 947, 997, 1014, 1886, 2578, 2661
TauI GCSGC 2 cut(s) 1300, 1430
TfiI GAWTC 7 cut(s) 238, 372, 917, 1050, 1568, 2079, 2614
Tru1I TTAA 8 cut(s) 105, 456, 651, 1059, 1779, 2376, 2627, 2643
Tru9I TTAA 8 cut(s) 105, 456, 651, 1059, 1779, 2376, 2627, 2643
TscAI CASTG 4 cut(s) 883, 1798, 2077, 2358
TseFI GTSAC 6 cut(s) 298, 628, 1325, 1366, 1653, 1761
TseI GCWGC 5 cut(s) 638, 1169, 1285, 1319, 2096
Tsp45I GTSAC 6 cut(s) 298, 628, 1325, 1366, 1653, 1761
TspGWI ACGGA 2 cut(s) 566, 1844
TspRI CASTG 4 cut(s) 883, 1798, 2077, 2358
Vha464I CTTAAG 2 cut(s) 104, 2375
VpaK11BI GGWCC 6 cut(s) 257, 461, 1195, 1261, 2290, 2724
VspI ATTAAT 3 cut(s) 456, 651, 1779
XagI CCTNNNNNAGG 1 cut(s) 2391
XapI RAATTY 7 cut(s) 267, 380, 492, 991, 1010, 1674, 2573
XbaI TCTAGA 1 cut(s) 368
XceI RCATGY 5 cut(s) 1345, 1367, 1741, 2185, 2735
XcmI CCANNNNNNNNNTGG 1 cut(s) 437
XmiI GTMKAC 2 cut(s) 138, 2801
XmnI GAANNNNTTC 1 cut(s) 2141
XspI CTAG 7 cut(s) 173, 369, 1647, 1806, 1865, 1910, 2189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.