Rw2G045990
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000071
Physical Location & Seq
Reverse (-)
73168203 .. 73172747
4545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G045990.1

Sequence Viewer

Length: 2703 bp
ATGAATGATATTGTCTCTTCAGAAGGAAGCAAATCAGATGCTTCCAACCCTTTCTTCATTCACCACTCTGATCATCTAGGGTTGGTATTGGTCACAAAACCTCTCAATGGCGATAATTATGCCACTTGGCGAAGAGCCATGTCAATCTCCTTGAATGCCAAGAATAAACTTGGTTTCGTCAATGGCACCATCAAGCCTCCTTCTTCAGAAACCAATCCTGACGATTATGCAGCATGGTCTCGTTGCAATGATATGGTTCACTCTTGGATTGTGAACTCGATTACTCCAGATATATCTGACAGTGAACAGCTTTCTGTTGCAGCCTACTACACTAAGTTGAAAGGTTTGTGGGATGAATTGGGTTCTTATACAGATTCAATATGCACTTGTGGAATTCTTATGATGAATCCTCTCCCATCGGTGCGTCAAGCCTACTCTTCTATTTGTCAACAGAAGAGACAACGTGCTCTTGGTGCTGCTCAAGGGTCAAATGACATGACTAGTGCTGCCATGGCTGTCAGGAATGGAAGAACAAATTCTTTTCCTCGCTCAAATCAACCAAATCGGCGTGCTGGTTCCTCTAATCGACGTCCCCACTGCTCTCATTGTAATGAAGATGGCCATTTTGTTGAAACTTGTTGGAAATTGCATGGTTATCCTCCTGGGCATGCAAAGCACAAATCTAAGAATATCGGAAATCGTGGAAGTGTGACCTCTGTGAACAATGTCTCTGAAAGCACAAGTATGGATGATTTGAAGCCAATTATCTCAGGTCTTTCAGATACACAGATTCAACAACTTTTGACAATGCTGAACGTAAATGGCAAAGGTAGTGACAGACAGACTTCTCAAGTTAATGCTGCAACAACACCAGGTTTGTCTAAAACTTTATCTCGCCGTTGGATACTTGATAGTGGCGCAACAGATCATATCGTTTCATCTTCAGACATATTGACACATAGCAATAAAAATCACATTTTGCCTCCTGTATTACTTCCGAGTGGAGAAAAGGCACACATAACTTCTACAGGATCAGTACCGTTGAATTCCTATTATTATCTCCATAACGATCTGGGTTCGAGGAGGACGATTGGTTTGGGTAAGCAACGTAACGGTCTCTACTATTTGGTGGCATTAGCGACGGAGAAAAACATGTCTAAACCACATCACTCATCACCCATTCAACCTGCTTGTCACATGACCATGTCCTCCACCAACCTTTGGCATTGTCATTGCGTCGCTGCCTCCCAAACGCCACCATCGTCTGTGGTTGTGCCGCCCCCACCCATTCAGGAGTTTCCGCCACCCCAAGCCGACCCGCAACCTCCTATACCAGACCTCCAGCCTCCTGATCCCGATCCACAACCTCTCCGTTGCTCTGCCCGGCCTACTCAGCTTTCCGTTCGATTACGTGATTATGTGTGTTCCCAGGTTACCTCCGCCTTCCCAAACTCGTCGTCTTCTTCTTCTCCAGGTTCTGTCAAAGGTACGCGCTATCCTCTGTGTAATTATCTCTCATATCATCGTTCCACTCCTTCGCATCGCTCCTTTATTGCTCAGATTACCCAGGCCACAGAACCCAGCAGTTATGCTGAAGCTGCATCCCATCCAGAATGGCAGGACGCCATGCGTTCTGAATTACGAGCATTGGAGGACAATCATACTTGGACTCTCACTTCTTTGCCGGCTGGGAAAACACCTATTGATTGTCGTTGGGTTTACAAGATCAAGCATCGCTCAGATGGTTCTATTGAACGGTACAAAGCCCGTTTGGTTGCCAAGGGCTTCACTCAGATGGAAGGTATCGATTATCATGAGACTTTCTCCCCTACTACAAAGATTATTTCTGTTCGTTGTTTATTATCCTTGGCTGCTGCTCAAAATTGGTCCATTCATCAGTTAGATGTCAATAATGCTTTCTTACATGGTGACCTTGATGAGGAAATTTTCATGTCACCTCCTCCTGCCTCTCGGCAATGGTTTGCAAAATTCTCTGATGCTATTCAATCAGCAGGGTATGTTCAATCTAAAGCAGATTACTCATTGTTCACTCGCAAACAGGGGAAGTCATTCACTGCTTTATTGATATATGTCGATGATATATTAATCACCGGTAATGATCCTATCACTATTTCTGGTTTAAAGAATTTTCTCAACAGCCGCTTTCGTATTAAGGATCTTGGTGATTTAAAGTACTTTCTTGGCATAGAAGTTTCTCGTTCCCCCAGTGGGGTTTATATTTCTCAGAGAAAGTATGCATTGGAAATCATCAGTGATGCAGGTTTATTGGGAGCAGCTCCCATTGATACTCCTATGGAGCGTGGATTGAAATTATCAGATAAAGGAGATCTTCTTAAGGATGCTTCACGCTACAGGAGATTAGTTGTGCATGTGTTGAGCAGGTTTATGCATGAGCCACGTAAACCTCATATGGAGGCAGCGTTACGAGTTGTTCGCTATCTCAAGAACACGCCAGGGCAAGCTTTGCACATTGCATCTAACCCTGTCTTTCATGAAAGGACTAGACATATAGAAATGGATTGTCATTATATCAGGGATAAGATTCAAGATGGTTCAGTAGCTACAAAGTATGTGAGCTCTTCATACCAATTGGCTGATGTTTTCACGAAAGCATTGGGAAAGGACGTATTCATTCCTTTGATCCGCAAGTTGGGAGCTCGAGACATTCACTCTCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_3 Retrotrans_gag

Protein Analysis

900

Amino Acids

99.71

Weight (kDa)

9.12

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 21 - 67 2.2e-20 gag-polypeptide of LTR copia-type
RVT_2 PF07727 553 - 657 7.4e-34 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2 PF07727 656 - 773 1.4e-21 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 592
Acc36I ACCTGC 3 cut(s) 1195, 2270, 2391
AccB1I GGYRCC 1 cut(s) 185
AccB7I CCANNNNNTGG 1 cut(s) 1221
AccII CGCG 1 cut(s) 1492
AciI CCGC 6 cut(s) 1277, 1301, 1319, 1440, 2161, 2665
AclWI GGATC 6 cut(s) 1039, 1346, 1352, 2114, 2184, 2656
AcoI YGGCCR 1 cut(s) 619
AcsI RAATTY 6 cut(s) 393, 535, 1045, 1944, 1988, 2146
AcuI CTGAAG 3 cut(s) 189, 927, 1614
AcyI GRCGYC 2 cut(s) 589, 1623
AfaI GTAC 4 cut(s) 1038, 1489, 1760, 2195
AfiI CCNNNNNNNGG 6 cut(s) 107, 1221, 1939, 2229, 2230, 2671
AflII CTTAAG 1 cut(s) 2354
AflIII ACRYGT 1 cut(s) 1152
AgeI ACCGGT 1 cut(s) 2111
AhlI ACTAGT 1 cut(s) 500
AjnI CCWGG 6 cut(s) 661, 871, 1428, 1471, 1566, 2473
AjuI GAANNNNNNNTTGG 2 cut(s) 38, 70
AluBI AGCT 8 cut(s) 310, 1396, 1598, 2297, 2483, 2582, 2598, 2678
AluI AGCT 8 cut(s) 310, 1396, 1598, 2297, 2483, 2582, 2598, 2678
Alw21I GWGCWC 3 cut(s) 469, 2600, 2680
Alw26I GTCTC 7 cut(s) 19, 243, 451, 733, 1121, 1811, 2676
AlwI GGATC 6 cut(s) 1039, 1346, 1352, 2114, 2184, 2656
AlwNI CAGNNNCTG 1 cut(s) 1478
Ama87I CYCGRG 1 cut(s) 2679
AoxI GGCC 3 cut(s) 619, 1385, 1569
ApoI RAATTY 6 cut(s) 393, 535, 1045, 1944, 1988, 2146
ArsI GACNNNNNNTTYG 4 cut(s) 1078, 1110, 1442, 1474
AseI ATTAAT 1 cut(s) 2105
AsiGI ACCGGT 1 cut(s) 2111
Asp700I GAANNNNTTC 2 cut(s) 535, 2069
AspLEI GCGC 2 cut(s) 920, 1494
AspS9I GGNCC 1 cut(s) 1887
AsuC2I CCSGG 1 cut(s) 1384
AsuHPI GGTGA 6 cut(s) 53, 1167, 1940, 1947, 2101, 2195
AvaI CYCGRG 1 cut(s) 2679
AvaII GGWCC 1 cut(s) 1887
BalI TGGCCA 1 cut(s) 621
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 2 cut(s) 2600, 2680
BbsI GAAGAC 1 cut(s) 1452
Bbv12I GWGCWC 3 cut(s) 469, 2600, 2680
BccI CCATC 8 cut(s) 197, 424, 611, 1267, 1614, 1736, 1789, 2564
BceAI ACGGC 1 cut(s) 882
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 6 cut(s) 663, 873, 1430, 1473, 1568, 2475
BciVI GTATCC 1 cut(s) 897
BclI TGATCA 1 cut(s) 70
BcnI CCSGG 1 cut(s) 1384
BcoDI GTCTC 7 cut(s) 19, 243, 451, 733, 1121, 1811, 2676
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 3 cut(s) 77, 501, 2523
BfmI CTRYAG 2 cut(s) 1026, 2371
BfrI CTTAAG 1 cut(s) 2354
BfuAI ACCTGC 3 cut(s) 1195, 2270, 2391
BfuI GTATCC 1 cut(s) 897
BglII AGATCT 1 cut(s) 2347
BmcAI AGTACT 1 cut(s) 2195
Bme1390I CCNGG 7 cut(s) 663, 873, 1384, 1430, 1473, 1568, 2475
Bme18I GGWCC 1 cut(s) 1887
BmeT110I CYCGRG 1 cut(s) 2679
BmgT120I GGNCC 1 cut(s) 1887
BmiI GGNNCC 2 cut(s) 187, 577
BmrFI CCNGG 7 cut(s) 663, 873, 1384, 1430, 1473, 1568, 2475
BmrI ACTGGG 1 cut(s) 2220
BmsI GCATC 8 cut(s) 28, 1549, 1610, 1741, 1987, 2266, 2350, 2504
BmuI ACTGGG 1 cut(s) 2220
BpiI GAAGAC 1 cut(s) 1452
BplI GAGNNNNNCTC 2 cut(s) 1808, 1840
BpmI CTGGAG 3 cut(s) 270, 1325, 1455
BpuEI CTTGAG 3 cut(s) 465, 834, 2447
BpuMI CCSGG 1 cut(s) 1384
Bsa29I ATCGAT 1 cut(s) 1806
BsaAI YACGTR 2 cut(s) 1412, 2420
BsaBI GATNNNNATC 1 cut(s) 1356
BsaHI GRCGYC 2 cut(s) 589, 1623
BsaI GGTCTC 2 cut(s) 243, 1121
BsaJI CCNNGG 7 cut(s) 510, 662, 1428, 1566, 1779, 1866, 2474
BsaWI WCCGGW 1 cut(s) 2111
BsaXI ACNNNNNCTCC 6 cut(s) 1323, 1353, 1353, 1383, 2426, 2456
Bsc4I CCNNNNNNNGG 6 cut(s) 107, 1221, 1939, 2229, 2230, 2671
Bse118I RCCGGY 2 cut(s) 1684, 2111
Bse1I ACTGG 1 cut(s) 2226
Bse3DI GCAATG 4 cut(s) 253, 1231, 1982, 2490
Bse8I GATNNNNATC 1 cut(s) 1356
BseBI CCWGG 6 cut(s) 663, 873, 1430, 1473, 1568, 2475
BseCI ATCGAT 1 cut(s) 1806
BseDI CCNNGG 7 cut(s) 510, 662, 1428, 1566, 1779, 1866, 2474
BseGI GGATG 5 cut(s) 358, 754, 1601, 1606, 2365
BseJI GATNNNNATC 1 cut(s) 1356
BseLI CCNNNNNNNGG 6 cut(s) 107, 1221, 1939, 2229, 2230, 2671
BseMI GCAATG 4 cut(s) 253, 1231, 1982, 2490
BseMII CTCAG 6 cut(s) 783, 1406, 1571, 1752, 1805, 2258
BseNI ACTGG 1 cut(s) 2226
BseRI GAGGAG 2 cut(s) 1096, 1950
BseYI CCCAGC 2 cut(s) 1580, 1688
Bsh1236I CGCG 1 cut(s) 1492
BshFI GGCC 3 cut(s) 621, 1387, 1571
BshNI GGYRCC 1 cut(s) 185
BshTI ACCGGT 1 cut(s) 2111
BshVI ATCGAT 1 cut(s) 1806
BsiHKAI GWGCWC 3 cut(s) 469, 2600, 2680
BsiHKCI CYCGRG 1 cut(s) 2679
BsiSI CCGG 3 cut(s) 1384, 1685, 2112
BslFI GGGAC 1 cut(s) 576
BslI CCNNNNNNNGG 6 cut(s) 107, 1221, 1939, 2229, 2230, 2671
BsmAI GTCTC 7 cut(s) 19, 243, 451, 733, 1121, 1811, 2676
BsmFI GGGAC 1 cut(s) 576
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 3 cut(s) 621, 1387, 1571
Bso31I GGTCTC 2 cut(s) 243, 1121
BsoBI CYCGRG 1 cut(s) 2679
Bsp1286I GDGCHC 3 cut(s) 469, 2600, 2680
Bsp19I CCATGG 1 cut(s) 510
BspACI CCGC 6 cut(s) 1277, 1301, 1319, 1440, 2161, 2665
BspANI GGCC 3 cut(s) 621, 1387, 1571
BspCNI CTCAG 6 cut(s) 782, 1405, 1570, 1751, 1804, 2257
BspDI ATCGAT 1 cut(s) 1806
BspFNI CGCG 1 cut(s) 1492
BspHI TCATGA 2 cut(s) 1813, 2512
BspLI GGNNCC 2 cut(s) 187, 577
BspMI ACCTGC 3 cut(s) 1195, 2270, 2391
BspPI GGATC 6 cut(s) 1039, 1346, 1352, 2114, 2184, 2656
BspQI GCTCTTC 2 cut(s) 127, 2605
BspT107I GGYRCC 1 cut(s) 185
BspTI CTTAAG 1 cut(s) 2354
BspTNI GGTCTC 2 cut(s) 243, 1121
BsrDI GCAATG 4 cut(s) 253, 1231, 1982, 2490
BsrFI RCCGGY 2 cut(s) 1684, 2111
BsrI ACTGG 1 cut(s) 2226
BssAI RCCGGY 2 cut(s) 1684, 2111
BssECI CCNNGG 7 cut(s) 510, 662, 1428, 1566, 1779, 1866, 2474
BssNI GRCGYC 2 cut(s) 589, 1623
BssT1I CCWWGG 3 cut(s) 510, 1779, 1866
Bst2UI CCWGG 6 cut(s) 663, 873, 1430, 1473, 1568, 2475
Bst4CI ACNGT 4 cut(s) 302, 1041, 1115, 1758
Bst6I CTCTTC 5 cut(s) 22, 127, 442, 449, 2605
BstACI GRCGYC 2 cut(s) 589, 1623
BstAFI CTTAAG 1 cut(s) 2354
BstAPI GCANNNNNTGC 1 cut(s) 2485
BstBAI YACGTR 2 cut(s) 1412, 2420
BstC8I GCNNGC 4 cut(s) 570, 669, 1686, 2481
BstDEI CTNAG 8 cut(s) 333, 684, 769, 1392, 1557, 1738, 1791, 2244
BstDSI CCRYGG 1 cut(s) 510
BstEII GGTNACC 2 cut(s) 1432, 1928
BstENI CCTNNNNNAGG 1 cut(s) 1937
BstF5I GGATG 5 cut(s) 358, 754, 1601, 1606, 2365
BstFNI CGCG 1 cut(s) 1492
BstHHI GCGC 2 cut(s) 920, 1494
BstMAI GTCTC 7 cut(s) 19, 243, 451, 733, 1121, 1811, 2676
BstMWI GCNNNNNNNGC 6 cut(s) 473, 512, 673, 1393, 1598, 2485
BstNI CCWGG 6 cut(s) 663, 873, 1430, 1473, 1568, 2475
BstNSI RCATGY 3 cut(s) 671, 1156, 2393
BstPI GGTNACC 2 cut(s) 1432, 1928
BstSCI CCNGG 7 cut(s) 661, 871, 1382, 1428, 1471, 1566, 2473
BstSFI CTRYAG 2 cut(s) 1026, 2371
BstUI CGCG 1 cut(s) 1492
BstV2I GAAGAC 1 cut(s) 1452
BstX2I RGATCY 2 cut(s) 2176, 2347
BstYI RGATCY 2 cut(s) 2176, 2347
Bsu15I ATCGAT 1 cut(s) 1806
BsuI GTATCC 1 cut(s) 897
BsuRI GGCC 3 cut(s) 621, 1387, 1571
BsuTUI ATCGAT 1 cut(s) 1806
BtgI CCRYGG 1 cut(s) 510
BtgZI GCGATG 2 cut(s) 1526, 1718
BtsCI GGATG 5 cut(s) 358, 754, 1601, 1606, 2365
BtsI GCAGTG 2 cut(s) 595, 2073
BtsIMutI CAGTG 5 cut(s) 307, 595, 2073, 2233, 2278
BveI ACCTGC 3 cut(s) 1195, 2270, 2391
Cac8I GCNNGC 4 cut(s) 570, 669, 1686, 2481
CaiI CAGNNNCTG 1 cut(s) 1478
CciI TCATGA 2 cut(s) 1813, 2512
CfoI GCGC 2 cut(s) 920, 1494
Cfr10I RCCGGY 2 cut(s) 1684, 2111
Cfr13I GGNCC 1 cut(s) 1887
ClaI ATCGAT 1 cut(s) 1806
CseI GACGC 3 cut(s) 413, 1225, 1631
CsiI ACCWGGT 1 cut(s) 871
Csp6I GTAC 4 cut(s) 1037, 1488, 1759, 2194
CspAI ACCGGT 1 cut(s) 2111
CviQI GTAC 4 cut(s) 1037, 1488, 1759, 2194
DdeI CTNAG 8 cut(s) 333, 684, 769, 1392, 1557, 1738, 1791, 2244
DraI TTTAAA 2 cut(s) 2142, 2190
EaeI YGGCCR 1 cut(s) 619
Eam1104I CTCTTC 5 cut(s) 22, 127, 442, 449, 2605
EarI CTCTTC 5 cut(s) 22, 127, 442, 449, 2605
EciI GGCGGA 2 cut(s) 1290, 1429
Ecl136II GAGCTC 2 cut(s) 2598, 2678
Eco130I CCWWGG 3 cut(s) 510, 1779, 1866
Eco24I GRGCYC 2 cut(s) 2600, 2680
Eco31I GGTCTC 2 cut(s) 243, 1121
Eco47I GGWCC 1 cut(s) 1887
Eco53kI GAGCTC 2 cut(s) 2598, 2678
Eco57I CTGAAG 3 cut(s) 189, 927, 1614
Eco88I CYCGRG 1 cut(s) 2679
Eco91I GGTNACC 2 cut(s) 1432, 1928
EcoICRI GAGCTC 2 cut(s) 2598, 2678
EcoNI CCTNNNNNAGG 1 cut(s) 1937
EcoO65I GGTNACC 2 cut(s) 1432, 1928
EcoRI GAATTC 2 cut(s) 393, 1045
EcoRII CCWGG 6 cut(s) 661, 871, 1428, 1471, 1566, 2473
EcoT14I CCWWGG 3 cut(s) 510, 1779, 1866
EcoT22I ATGCAT 2 cut(s) 2260, 2412
EcoT38I GRGCYC 2 cut(s) 2600, 2680
ErhI CCWWGG 3 cut(s) 510, 1779, 1866
FalI AAGNNNNNCTT 2 cut(s) 2334, 2366
FaqI GGGAC 1 cut(s) 576
FauI CCCGC 1 cut(s) 1326
FauNDI CATATG 1 cut(s) 2430
FbaI TGATCA 1 cut(s) 70
FokI GGATG 5 cut(s) 365, 761, 1588, 1593, 2372
FriOI GRGCYC 2 cut(s) 2600, 2680
FspBI CTAG 3 cut(s) 77, 501, 2523
GlaI GCGC 2 cut(s) 919, 1493
GsaI CCCAGC 2 cut(s) 1584, 1692
GsuI CTGGAG 3 cut(s) 270, 1325, 1455
HaeIII GGCC 3 cut(s) 621, 1387, 1571
HapII CCGG 3 cut(s) 1384, 1685, 2112
HgaI GACGC 3 cut(s) 413, 1225, 1631
HhaI GCGC 2 cut(s) 920, 1494
Hin1I GRCGYC 2 cut(s) 589, 1623
Hin6I GCGC 2 cut(s) 918, 1492
HinP1I GCGC 2 cut(s) 918, 1492
HincII GTYRAC 1 cut(s) 449
HindII GTYRAC 1 cut(s) 449
HindIII AAGCTT 1 cut(s) 2481
HinfI GANTC 5 cut(s) 374, 406, 790, 1669, 2563
HpaII CCGG 3 cut(s) 1384, 1685, 2112
HphI GGTGA 6 cut(s) 53, 1167, 1940, 1947, 2101, 2195
Hpy166II GTNNAC 8 cut(s) 259, 274, 305, 449, 721, 1720, 2049, 2423
Hpy8I GTNNAC 8 cut(s) 259, 274, 305, 449, 721, 1720, 2049, 2423
Hpy99I CGWCG 4 cut(s) 591, 1144, 1241, 1459
HpyAV CCTTC 5 cut(s) 17, 210, 1453, 1545, 1793
HpyCH4III ACNGT 4 cut(s) 302, 1041, 1115, 1758
HpyCH4IV ACGT 7 cut(s) 463, 589, 816, 1108, 1411, 2419, 2646
HpyF10VI GCNNNNNNNGC 6 cut(s) 473, 512, 673, 1393, 1598, 2485
HpyF3I CTNAG 8 cut(s) 333, 684, 769, 1392, 1557, 1738, 1791, 2244
HpySE526I ACGT 7 cut(s) 463, 589, 816, 1108, 1411, 2419, 2646
Hsp92I GRCGYC 2 cut(s) 589, 1623
HspAI GCGC 2 cut(s) 918, 1492
KroI GCCGGC 1 cut(s) 1684
KroNI GCCGGC 1 cut(s) 1686
Ksp22I TGATCA 1 cut(s) 70
LguI GCTCTTC 2 cut(s) 127, 2605
LmnI GCTCC 5 cut(s) 1550, 2291, 2302, 2317, 2675
LweI GCATC 8 cut(s) 28, 1549, 1610, 1741, 1987, 2266, 2350, 2504
MabI ACCWGGT 1 cut(s) 871
MaeI CTAG 3 cut(s) 77, 501, 2523
MaeII ACGT 7 cut(s) 463, 589, 816, 1108, 1411, 2419, 2646
MaeIII GTNAC 9 cut(s) 91, 709, 833, 1109, 1193, 1432, 1928, 1953, 2442
MfeI CAATTG 1 cut(s) 2609
MflI RGATCY 2 cut(s) 2176, 2347
MhlI GDGCHC 3 cut(s) 469, 2600, 2680
MlsI TGGCCA 1 cut(s) 621
MluNI TGGCCA 1 cut(s) 621
MlyI GAGTC 1 cut(s) 1663
MmeI TCCRAC 3 cut(s) 69, 620, 881
Mox20I TGGCCA 1 cut(s) 621
Mph1103I ATGCAT 2 cut(s) 2260, 2412
MroNI GCCGGC 1 cut(s) 1684
MroXI GAANNNNTTC 2 cut(s) 535, 2069
MscI TGGCCA 1 cut(s) 621
MseI TTAA 6 cut(s) 855, 2105, 2141, 2172, 2189, 2355
MslI CAYNNNNRTG 4 cut(s) 609, 743, 1202, 1793
Msp20I TGGCCA 1 cut(s) 621
MspCI CTTAAG 1 cut(s) 2354
MspI CCGG 3 cut(s) 1384, 1685, 2112
MspR9I CCNGG 7 cut(s) 663, 873, 1384, 1430, 1473, 1568, 2475
MunI CAATTG 1 cut(s) 2609
Mva1269I GAATGC 1 cut(s) 160
MvaI CCWGG 6 cut(s) 663, 873, 1430, 1473, 1568, 2475
MvnI CGCG 1 cut(s) 1492
MwoI GCNNNNNNNGC 6 cut(s) 473, 512, 673, 1393, 1598, 2485
NaeI GCCGGC 1 cut(s) 1686
NciI CCSGG 1 cut(s) 1384
NcoI CCATGG 1 cut(s) 510
NdeI CATATG 1 cut(s) 2430
NgoMIV GCCGGC 1 cut(s) 1684
NlaIV GGNNCC 2 cut(s) 187, 577
NmeAIII GCCGAG 1 cut(s) 1951
NmuCI GTSAC 6 cut(s) 91, 709, 833, 1193, 1928, 1953
NsiI ATGCAT 2 cut(s) 2260, 2412
NspI RCATGY 3 cut(s) 671, 1156, 2393
PaeI GCATGC 1 cut(s) 671
PaeR7I CTCGAG 1 cut(s) 2679
PagI TCATGA 2 cut(s) 1813, 2512
PciI ACATGT 1 cut(s) 1152
PciSI GCTCTTC 2 cut(s) 127, 2605
PcsI WCGNNNNNNNCGW 2 cut(s) 1085, 1259
PctI GAATGC 1 cut(s) 160
PdiI GCCGGC 1 cut(s) 1686
PdmI GAANNNNTTC 2 cut(s) 535, 2069
PfeI GAWTC 4 cut(s) 374, 406, 790, 2563
PflFI GACNNNGTC 1 cut(s) 1204
PflMI CCANNNNNTGG 1 cut(s) 1221
PinAI ACCGGT 1 cut(s) 2111
PleI GAGTC 1 cut(s) 1663
PpsI GAGTC 1 cut(s) 1663
Ppu21I YACGTR 2 cut(s) 1412, 2420
PscI ACATGT 1 cut(s) 1152
PshBI ATTAAT 1 cut(s) 2105
Psp124BI GAGCTC 2 cut(s) 2600, 2680
Psp6I CCWGG 6 cut(s) 661, 871, 1428, 1471, 1566, 2473
PspEI GGTNACC 2 cut(s) 1432, 1928
PspFI CCCAGC 2 cut(s) 1580, 1688
PspGI CCWGG 6 cut(s) 661, 871, 1428, 1471, 1566, 2473
PspN4I GGNNCC 2 cut(s) 187, 577
PspPI GGNCC 1 cut(s) 1887
PstNI CAGNNNCTG 1 cut(s) 1478
PsuI RGATCY 2 cut(s) 2176, 2347
PsyI GACNNNGTC 1 cut(s) 1204
RsaI GTAC 4 cut(s) 1038, 1489, 1760, 2195
RsaNI GTAC 4 cut(s) 1037, 1488, 1759, 2194
RseI CAYNNNNRTG 4 cut(s) 609, 743, 1202, 1793
SacI GAGCTC 2 cut(s) 2600, 2680
SapI GCTCTTC 2 cut(s) 127, 2605
SaqAI TTAA 6 cut(s) 855, 2105, 2141, 2172, 2189, 2355
Sau96I GGNCC 1 cut(s) 1887
ScaI AGTACT 1 cut(s) 2195
SchI GAGTC 1 cut(s) 1663
ScrFI CCNGG 7 cut(s) 663, 873, 1384, 1430, 1473, 1568, 2475
SduI GDGCHC 3 cut(s) 469, 2600, 2680
SexAI ACCWGGT 1 cut(s) 871
SfaNI GCATC 8 cut(s) 28, 1549, 1610, 1741, 1987, 2266, 2350, 2504
SfcI CTRYAG 2 cut(s) 1026, 2371
Sfr274I CTCGAG 1 cut(s) 2679
SinI GGWCC 1 cut(s) 1887
SlaI CTCGAG 1 cut(s) 2679
SmiMI CAYNNNNRTG 4 cut(s) 609, 743, 1202, 1793
SmlI CTYRAG 5 cut(s) 480, 849, 2354, 2462, 2679
SmoI CTYRAG 5 cut(s) 480, 849, 2354, 2462, 2679
SpeI ACTAGT 1 cut(s) 500
SphI GCATGC 1 cut(s) 671
SsiI CCGC 6 cut(s) 1277, 1301, 1319, 1440, 2161, 2665
SspMI CTAG 3 cut(s) 77, 501, 2523
SstI GAGCTC 2 cut(s) 2600, 2680
StyD4I CCNGG 7 cut(s) 661, 871, 1382, 1428, 1471, 1566, 2473
StyI CCWWGG 3 cut(s) 510, 1779, 1866
TaaI ACNGT 4 cut(s) 302, 1041, 1115, 1758
TaiI ACGT 7 cut(s) 466, 592, 819, 1111, 1414, 2422, 2649
TaqI TCGA 7 cut(s) 278, 586, 1079, 1405, 1806, 2094, 2680
TatI WGTACW 1 cut(s) 2193
TauI GCSGC 2 cut(s) 1279, 2163
TfiI GAWTC 4 cut(s) 374, 406, 790, 2563
Tru1I TTAA 6 cut(s) 855, 2105, 2141, 2172, 2189, 2355
Tru9I TTAA 6 cut(s) 855, 2105, 2141, 2172, 2189, 2355
TscAI CASTG 5 cut(s) 307, 602, 2080, 2233, 2278
TseFI GTSAC 6 cut(s) 91, 709, 833, 1193, 1928, 1953
Tsp45I GTSAC 6 cut(s) 91, 709, 833, 1193, 1928, 1953
TspGWI ACGGA 3 cut(s) 1157, 1361, 1390
TspRI CASTG 5 cut(s) 307, 602, 2080, 2233, 2278
Tth111I GACNNNGTC 1 cut(s) 1204
Van91I CCANNNNNTGG 1 cut(s) 1221
Vha464I CTTAAG 1 cut(s) 2354
VpaK11BI GGWCC 1 cut(s) 1887
VspI ATTAAT 1 cut(s) 2105
XagI CCTNNNNNAGG 1 cut(s) 1937
XapI RAATTY 6 cut(s) 393, 535, 1045, 1944, 1988, 2146
XceI RCATGY 3 cut(s) 671, 1156, 2393
XhoI CTCGAG 1 cut(s) 2679
XmnI GAANNNNTTC 2 cut(s) 535, 2069
XspI CTAG 3 cut(s) 77, 501, 2523
ZraI GACGTC 1 cut(s) 590
ZrmI AGTACT 1 cut(s) 2195
Zsp2I ATGCAT 2 cut(s) 2260, 2412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.