RchiOBHm_Chr1g0320771

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000071
Physical Location & Seq
Forward (+)
8339861 .. 8343718
3858 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55089

Sequence Viewer

Length: 3858 bp
ATGGGACTTAATGAGAATTTTTCACAGACCCGCAGCAACATTATAGGGTTGGATCCACTTCTGAACCTGAACAAAACGTATGCGATGGTACTACGCCAAGAGAAGCAGGCAGAGACTATTGTTGGCAAGTCCACGGCACCACCAGAATCCTCTGCTTTTTCAGTGAAGAAAGTGACTCGTGATTTCAAGTCTGTAGAAGGAGAGGCCAAATTTTGTGACAAATGCAACATGACCAACCACAACACAAAGAACTGCAGAGCTCATCTCAAATGCACATACTGCAATTGGAAGGGACATACCTATGACTACTGCCGAAGGAGGAAGAATGCTACTGAAAGTGGCCAAGCAAAATCAAAAGCAAATCATGTAGCTCTTCAAGATGATCAAAAGGAAGCTGCGACTGATTTCCCATTTTCACGCGAAGAGTGTCATCAACTTCTCAATCAGCTCCTAACCAAAACGAAGGCTGCCTCAGCCAATTTGGTCGGTAACATTCCCAACTATGAAGAGCTTTCAGGTAAACTTCTCTCCCTTGCACAGAATAGTAGAGAGCCCATATGGATATTAGATAGTGGTGCAAGCGATCATATTGTTTGTAATCCCAGCTTGCTTACCTCATCCAAATCAGTCTCTAATCGTTGGGTCAAGCTTCCTGACGGTACCACAGCACGTGTTACTCATGTTGGCACCGTAGTCTTCTCTCCACATTTTGTGCTTCACAATGTCCTATTTGTCCCTTTGTTTTACCTTAACTTGATCTCTGTGAGCAAACTAGCATTCGACTCTTTTCATATTACCATATTTCTAAGACAAATTTGTGTCATACAGGACCTACAATCGGGGAAGATGATTGGGACGGGAACTGAGAAGGAGGGACTCTACCGACTCAATCTACCACAGAAAGGAACATGCAATGCGGTACACACCAACTCGGACCCAAACAATTTATGGCACCAACGACTTGGTCATCCCTCAAATAAAGCATCTTCATTGTTTTCATTCCTTGCAAATAAAGCTTGTTATTCAAGCTCCTGTTCTATTTGTCCTTTAGCCAAACTTACTAGACAACCGTTTCCATTGAGTACTACTCGTAGTGCATCATGTTTTGATTTAATTCACATTGATATTTGGGGAGGTTATCATGTTCCAACTTTTTCTGGTGCACAATATTTTCTAACAATTGTTGATGACCATTCACGAAGCACTTGGGTGTATTTGATGAAACACAAGTCTGAAGCACGCACCCTCCTCATCCATTTTATTCATTTGGCTGCAAATCAGTTTGGTAAAAATGTTATGGTCGTTAGGAGTGACAATGGTCCAGAGTTTAAGATTCCCCAGTTTTATTCTTCTAAGGGCATTATCCACCAAACTAGCTGCGTCAACACACCACAACAAAATGGTGTTGCTGAACGCAAACATCGCCACTTGCTCAACATGGCCAGGGCCCTTCTTTTCCAAGCTAATTTGCCAAAACCTTTTTGGGGGGATGCCATACTCACAGCAGCTTACTTGATTAATCGAACACCAACTCCAATTCTTCAGGGAAAAACACCTTTTGAAAAACTTTTCCATAAGGAACCTAGCTATTCTCATTTAAGAGTCTTTGGCTGTCAATGCTTTGTATCCACACATCCAACTCGTCCTAGTAAGTTTGATCCTCGATCAATGGAGTGCGTTTTTCTTGGCTATCCACACGGCCAAAAGGGGTATAAAGTTTATAACTTGACAACTAAGAAATCCCTAGTTTCAAGGGATGTGATTTTCTTTGAGAATGCCTTTCCTTTCCCTAAAAATTCGGAGTCTTTTCCATCTCAAAATACCGATTTGTTTCCTTCAATACCTCGTTTGGCCCATTATGATAATCCTTCAATTCCTAAAATTCCTCCTTCAAGTCCCACACATCACTCTCCACCTATGATCTCTCCAAATCCCCAGTCTTCCGCTGAGCAATATCTTAATTCTCCTTCCAAATCTCTTTCATCTACAGACCCCGTATCTTCTGACATCACTCTTCCTAACTTGGACACAATCTCCTCTGATCACATTCCATCACTTTCGCCTCCAGAACAAACTCCACCACGACCACGCAAGTCTACTCGCGCTACCAAGCTCCCTACCGCATTGCAGGACTTTCACATTGATGCGGCCCTCCCCACACGTCTCGCTCCGTCTTCTTCTTCGAACGAGGTCACAACTCCTGGTACGGCTCATAGTCTCTCACATGTTCTTTCTTATGCCAATCTTTCTTCTCCTCATAGAACCTTTACTGCTAACATTACCCTGCAAAGAGAACCCACATCTTTTTCTCAGGCAGTAAAAGATCCAAAATGGAGAGAAGCTATGCGGCTAGAGGTTCAAGCGCTGCAAGATAACAAAACCTGGAGCCTCGTTCCACCACCGGCTCACAAACGACCGATCGGTTGTAAATGGGTGTACAAAATTAAGTACAATCCCGATGGAACTATTGAACGTTATAAGGCCAGGTTAGTGGCCAAGGGTTACAGCCAAGTGGAAGGCCTTGATTATAGGGAGACATTTGCACCAGTTGCCAAACTCACCACAGTCCGAGTCTTGCTCAGTCTCGCAGCTCAACAAAATTGGCATCTCCACCAACTGGATGTGAATAATGCGTTCCTCAATGGAGATCTCCATGAAGATGTATACATGCACCTTCCTCCTGGCTTTGAACGAAAGGGGGAGCATAAGGTCTGTAAACTACACAAATCATTATACGGCTTGAGGCAAGCCTCCAAGCAATGGTTTCTGAAACTCTCCTCAGCCCTCAAATCAGCCGGTTTCAAGCAATCATGGTCTGATTATTCCATGTTCGTCCGAAGCCATCAAGGTACCTTTACAGCTTTGTTGGTTTATGTGGACGATGTAATTCTTGCAGGCAACAATTTAGATGATATTATAAGGACCAAAAGCTTTCTATCAAGCCATTTCAAACTGAAGGATATGGGACAGTTGAAATATTTTCTTGGATTGGAAGTAGCAAGGTCCAAGCATGGCATTGCATTAAGTCAAAGAAAGTATGCATTGGAAATACTTGAAGACACAGGATTTCTTGGTGCAAAACCTTCACGTTTTCCACTTGAGCAAAATATAATCCTCACACAAGAAGATGGAAGATTGTTAGAAGACGCATCTCAATATCGAAGGCTTGTTGGACGCCTCATTTATCAGACAATCACAAGACCGGACCTCGTGTATGCAGTTCATATACTTAGTCAGTTCATGGATAAACCAAGACAGCCTCACTTAGATGCAGCTCACAAGGTGTTGAGATACCTCAAGCAGACACCAGGTCAGGGTATTTTCCTACCTTCAAAAGGGCCATTGGAATTAAGTGCATATTGTGATGCAGATTGGGCGCGCTGCAAAGACACTAGAAGGTCAACAACGGGTTATTGCATCTTCCTCGGACATGCTCCCATTTCATGGAAAACAAAGAAACAAAGAACCGTCTCACGTTCGAGTGCAGAAGCCGAGTATCGCTCTATGGCTACTACTTGTTGTGAGATAACATGGTTGCAATACATTTTGAAAGACTTGAATATTCAACATTTGCAGCCAGTCAAGTTGTTCTGCGATAATAAGGCTGCCATACACATAGCGTCAAATCCTGTGTTCCACGAAAGGACCAAGCATATAGAAATAGACTGTCATGTCGTAAGAGAGAAAGTGCAGAGAGGATTGATTCAAACAGAACATATAAGAACCAAGGAGCAACCAGCAGACATTTTCACGAAGCCCCTGAGTTCAGAACAGTTTTCATTGTTGTTGGGCAAGTTAGGAGTGATCAATATCCACTCTAACTTGAGGGGGAGTATTGAAGGAAAGAATCACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2

Protein Analysis

1285

Amino Acids

144.85

Weight (kDa)

9.43

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 187 - 259 1e-13 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 292 - 352 3.3e-08 GAG-pre-integrase domain
rve PF00665 371 - 464 3.8e-10 Integrase core domain
SH3_retrovirus PF25597 538 - 600 3.8e-23 Retroviral polymerase SH3-like domain
RVT_2 PF07727 792 - 1034 6.3e-74 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1722, 2478, 2918
AasI GACNNNNNNGTC 1 cut(s) 3671
Acc65I GGTACC 2 cut(s) 659, 2849
AccB1I GGYRCC 5 cut(s) 136, 659, 686, 951, 2849
AccB7I CCANNNNNTGG 3 cut(s) 2617, 2760, 3444
AccI GTMKAC 2 cut(s) 2096, 2664
AccII CGCG 3 cut(s) 420, 2103, 3379
AciI CCGC 6 cut(s) 31, 917, 1944, 2121, 2147, 2347
AclI AACGTT 1 cut(s) 2473
AclWI GGATC 4 cut(s) 47, 60, 1652, 2318
AcoI YGGCCR 4 cut(s) 340, 1440, 1699, 2493
AcsI RAATTY 5 cut(s) 16, 209, 813, 1795, 1881
AcuI CTGAAG 3 cut(s) 1254, 1526, 2975
AcvI CACGTG 1 cut(s) 671
AcyI GRCGYC 1 cut(s) 3175
AdeI CACNNNGTG 1 cut(s) 3283
AfaI GTAC 8 cut(s) 90, 661, 921, 1084, 2206, 2438, 2450, 2851
AfeI AGCGCT 1 cut(s) 2364
AfiI CCNNNNNNNGG 8 cut(s) 838, 902, 1484, 2617, 2760, 3314, 3335, 3444
AflIII ACRYGT 3 cut(s) 670, 2159, 2224
AhdI GACNNNNNGTC 1 cut(s) 3309
AjiI CACGTC 1 cut(s) 2162
AjnI CCWGG 6 cut(s) 1442, 2200, 2381, 2483, 2680, 3307
AleI CACNNNNGTG 1 cut(s) 1208
AloI GAACNNNNNNTCC 2 cut(s) 1516, 1548
Alw21I GWGCWC 2 cut(s) 262, 1165
Alw26I GTCTC 7 cut(s) 107, 634, 2168, 2222, 2528, 2588, 3475
Alw44I GTGCAC 1 cut(s) 1161
AlwI GGATC 4 cut(s) 47, 60, 1652, 2318
Aor51HI AGCGCT 1 cut(s) 2364
ApaI GGGCCC 1 cut(s) 1450
ApaLI GTGCAC 1 cut(s) 1161
ApoI RAATTY 5 cut(s) 16, 209, 813, 1795, 1881
ArsI GACNNNNNNTTYG 2 cut(s) 1922, 1954
AseI ATTAAT 1 cut(s) 1518
Asp718I GGTACC 2 cut(s) 659, 2849
AspLEI GCGC 4 cut(s) 2105, 2365, 3379, 3381
AsuHPI GGTGA 1 cut(s) 2551
AsuII TTCGAA 1 cut(s) 2183
AvaII GGWCC 7 cut(s) 829, 934, 1319, 2922, 3003, 3205, 3645
BaeGI GKGCMC 2 cut(s) 1165, 1450
BalI TGGCCA 3 cut(s) 342, 1442, 2495
BamHI GGATCC 1 cut(s) 52
BanI GGYRCC 5 cut(s) 136, 659, 686, 951, 2849
BanII GRGCYC 3 cut(s) 262, 555, 1450
BauI CACGAG 2 cut(s) 177, 3209
BbrPI CACGTG 1 cut(s) 671
BbsI GAAGAC 5 cut(s) 688, 1932, 2166, 3063, 3150
Bbv12I GWGCWC 2 cut(s) 262, 1165
BbvCI CCTCAGC 2 cut(s) 472, 2779
BccI CCATC 6 cut(s) 79, 1819, 2059, 2453, 2850, 3122
BceAI ACGGC 4 cut(s) 150, 1714, 2223, 2752
BcgI CGANNNNNNTGC 2 cut(s) 3406, 3440
BciT130I CCWGG 6 cut(s) 1444, 2202, 2383, 2485, 2682, 3309
BciVI GTATCC 1 cut(s) 1636
BclI TGATCA 3 cut(s) 382, 2041, 3804
BcoDI GTCTC 7 cut(s) 107, 634, 2168, 2222, 2528, 2588, 3475
BfaI CTAG 8 cut(s) 773, 1062, 1374, 1584, 1647, 1745, 2351, 3393
BfmI CTRYAG 3 cut(s) 192, 253, 1986
BfoI RGCGCY 1 cut(s) 2366
BfuI GTATCC 1 cut(s) 1636
BglII AGATCT 1 cut(s) 2647
BlpI GCTNAGC 1 cut(s) 1947
BmcAI AGTACT 1 cut(s) 1084
Bme1390I CCNGG 6 cut(s) 1444, 2202, 2383, 2485, 2682, 3309
Bme18I GGWCC 7 cut(s) 829, 934, 1319, 2922, 3003, 3205, 3645
BmeRI GACNNNNNGTC 1 cut(s) 3309
BmgBI CACGTC 1 cut(s) 2162
BmrFI CCNGG 6 cut(s) 1444, 2202, 2383, 2485, 2682, 3309
BmrI ACTGGG 2 cut(s) 1333, 1930
BmsI GCATC 9 cut(s) 992, 1106, 1480, 2134, 2614, 3158, 3259, 3355, 3426
BmuI ACTGGG 2 cut(s) 1333, 1930
BoxI GACNNNNGTC 1 cut(s) 1317
BpiI GAAGAC 5 cut(s) 688, 1932, 2166, 3063, 3150
BplI GAGNNNNNCTC 8 cut(s) 249, 281, 1072, 1104, 2562, 2594, 3485, 3517
BpmI CTGGAG 2 cut(s) 2049, 2404
Bpu10I CCTNAGC 2 cut(s) 472, 2779
Bpu1102I GCTNAGC 1 cut(s) 1947
Bpu14I TTCGAA 1 cut(s) 2183
BpuEI CTTGAG 4 cut(s) 2761, 3119, 3281, 3844
BsaAI YACGTR 1 cut(s) 671
BsaBI GATNNNNATC 1 cut(s) 3809
BsaHI GRCGYC 1 cut(s) 3175
BsaJI CCNNGG 5 cut(s) 132, 1443, 2496, 3424, 3726
BsaWI WCCGGW 1 cut(s) 3202
BsaXI ACNNNNNCTCC 6 cut(s) 1230, 1260, 1516, 1546, 2018, 2048
Bsc4I CCNNNNNNNGG 8 cut(s) 838, 902, 1484, 2617, 2760, 3314, 3335, 3444
Bse118I RCCGGY 2 cut(s) 2401, 2795
Bse1I ACTGG 5 cut(s) 1339, 1936, 2546, 2622, 3578
Bse3DI GCAATG 4 cut(s) 919, 2123, 2765, 3015
Bse8I GATNNNNATC 1 cut(s) 3809
BseBI CCWGG 6 cut(s) 1444, 2202, 2383, 2485, 2682, 3309
BseDI CCNNGG 5 cut(s) 132, 1443, 2496, 3424, 3726
BseGI GGATG 7 cut(s) 617, 967, 1251, 1495, 1633, 1762, 2626
BseJI GATNNNNATC 1 cut(s) 3809
BseLI CCNNNNNNNGG 8 cut(s) 838, 902, 1484, 2617, 2760, 3314, 3335, 3444
BseMI GCAATG 4 cut(s) 919, 2123, 2765, 3015
BseMII CTCAG 7 cut(s) 486, 855, 1938, 2324, 2593, 2793, 3752
BseNI ACTGG 5 cut(s) 1339, 1936, 2546, 2622, 3578
BsePI GCGCGC 1 cut(s) 3377
BseRI GAGGAG 4 cut(s) 1238, 2026, 2244, 2767
BseSI GKGCMC 2 cut(s) 1165, 1450
BseYI CCCAGC 1 cut(s) 602
BsgI GTGCAG 2 cut(s) 3504, 3710
Bsh1236I CGCG 3 cut(s) 420, 2103, 3379
Bsh1285I CGRYCG 2 cut(s) 2417, 2421
BshNI GGYRCC 5 cut(s) 136, 659, 686, 951, 2849
BsiEI CGRYCG 2 cut(s) 2417, 2421
BsiHKAI GWGCWC 2 cut(s) 262, 1165
BsiSI CCGG 3 cut(s) 2402, 2796, 3203
BslFI GGGAC 7 cut(s) 18, 306, 719, 868, 888, 1881, 2979
BslI CCNNNNNNNGG 8 cut(s) 838, 902, 1484, 2617, 2760, 3314, 3335, 3444
BsmAI GTCTC 7 cut(s) 107, 634, 2168, 2222, 2528, 2588, 3475
BsmBI CGTCTC 2 cut(s) 2168, 3475
BsmFI GGGAC 7 cut(s) 18, 306, 719, 868, 888, 1881, 2979
BsmI GAATGC 3 cut(s) 331, 776, 1780
Bsp119I TTCGAA 1 cut(s) 2183
Bsp120I GGGCCC 1 cut(s) 1446
Bsp1286I GDGCHC 4 cut(s) 262, 555, 1165, 1450
Bsp1407I TGTACA 1 cut(s) 2436
Bsp1720I GCTNAGC 1 cut(s) 1947
BspACI CCGC 6 cut(s) 31, 917, 1944, 2121, 2147, 2347
BspCNI CTCAG 7 cut(s) 485, 856, 1939, 2323, 2592, 2792, 3753
BspFNI CGCG 3 cut(s) 420, 2103, 3379
BspMAI CTGCAG 1 cut(s) 257
BspPI GGATC 4 cut(s) 47, 60, 1652, 2318
BspQI GCTCTTC 2 cut(s) 378, 501
BspT104I TTCGAA 1 cut(s) 2183
BspT107I GGYRCC 5 cut(s) 136, 659, 686, 951, 2849
BsrDI GCAATG 4 cut(s) 919, 2123, 2765, 3015
BsrFI RCCGGY 2 cut(s) 2401, 2795
BsrGI TGTACA 1 cut(s) 2436
BsrI ACTGG 5 cut(s) 1339, 1936, 2546, 2622, 3578
BssAI RCCGGY 2 cut(s) 2401, 2795
BssECI CCNNGG 5 cut(s) 132, 1443, 2496, 3424, 3726
BssHII GCGCGC 1 cut(s) 3377
BssNAI GTATAC 1 cut(s) 2665
BssNI GRCGYC 1 cut(s) 3175
BssSI CACGAG 2 cut(s) 177, 3209
BssT1I CCWWGG 2 cut(s) 2496, 3726
Bst1107I GTATAC 1 cut(s) 2665
Bst2BI CACGAG 2 cut(s) 177, 3209
Bst2UI CCWGG 6 cut(s) 1444, 2202, 2383, 2485, 2682, 3309
Bst4CI ACNGT 8 cut(s) 659, 691, 1071, 2566, 2970, 3469, 3668, 3774
Bst6I CTCTTC 4 cut(s) 378, 417, 501, 2019
BstACI GRCGYC 1 cut(s) 3175
BstAPI GCANNNNNTGC 2 cut(s) 279, 2549
BstAUI TGTACA 1 cut(s) 2436
BstBAI YACGTR 1 cut(s) 671
BstBI TTCGAA 1 cut(s) 2183
BstC8I GCNNGC 7 cut(s) 108, 580, 608, 1240, 2748, 2896, 3379
BstDSI CCRYGG 1 cut(s) 132
BstENI CCTNNNNNAGG 1 cut(s) 3333
BstF5I GGATG 7 cut(s) 617, 967, 1251, 1495, 1633, 1762, 2626
BstFNI CGCG 3 cut(s) 420, 2103, 3379
BstH2I RGCGCY 1 cut(s) 2366
BstHHI GCGC 4 cut(s) 2105, 2365, 3379, 3381
BstMAI GTCTC 7 cut(s) 107, 634, 2168, 2222, 2528, 2588, 3475
BstMCI CGRYCG 2 cut(s) 2417, 2421
BstMWI GCNNNNNNNGC 7 cut(s) 279, 473, 1013, 1422, 1617, 2549, 3374
BstNI CCWGG 6 cut(s) 1444, 2202, 2383, 2485, 2682, 3309
BstNSI RCATGY 4 cut(s) 912, 2228, 2671, 3434
BstPAI GACNNNNGTC 1 cut(s) 1317
BstSCI CCNGG 6 cut(s) 1442, 2200, 2381, 2483, 2680, 3307
BstSFI CTRYAG 3 cut(s) 192, 253, 1986
BstSLI GKGCMC 2 cut(s) 1165, 1450
BstUI CGCG 3 cut(s) 420, 2103, 3379
BstV2I GAAGAC 5 cut(s) 688, 1932, 2166, 3063, 3150
BstX2I RGATCY 3 cut(s) 52, 2323, 2647
BstXI CCANNNNNNTGG 2 cut(s) 962, 2491
BstYI RGATCY 3 cut(s) 52, 2323, 2647
BstZ17I GTATAC 1 cut(s) 2665
BsuI GTATCC 1 cut(s) 1636
BtgI CCRYGG 1 cut(s) 132
BtgZI GCGATG 2 cut(s) 98, 1406
BtrI CACGTC 1 cut(s) 2162
BtsCI GGATG 7 cut(s) 617, 967, 1251, 1495, 1633, 1762, 2626
BtsIMutI CAGTG 1 cut(s) 168
Cac8I GCNNGC 7 cut(s) 108, 580, 608, 1240, 2748, 2896, 3379
CfoI GCGC 4 cut(s) 2105, 2365, 3379, 3381
Cfr10I RCCGGY 2 cut(s) 2401, 2795
CseI GACGC 4 cut(s) 1369, 3155, 3183, 3609
CsiI ACCWGGT 1 cut(s) 3307
Csp6I GTAC 8 cut(s) 89, 660, 920, 1083, 2205, 2437, 2449, 2850
CviQI GTAC 8 cut(s) 89, 660, 920, 1083, 2205, 2437, 2449, 2850
DraIII CACNNNGTG 1 cut(s) 3283
DrdI GACNNNNNNGTC 1 cut(s) 3671
DriI GACNNNNNGTC 1 cut(s) 3309
DseDI GACNNNNNNGTC 1 cut(s) 3671
EaeI YGGCCR 4 cut(s) 340, 1440, 1699, 2493
Eam1104I CTCTTC 4 cut(s) 378, 417, 501, 2019
Eam1105I GACNNNNNGTC 1 cut(s) 3309
EarI CTCTTC 4 cut(s) 378, 417, 501, 2019
Ecl136II GAGCTC 1 cut(s) 260
Eco130I CCWWGG 2 cut(s) 2496, 3726
Eco147I AGGCCT 1 cut(s) 2520
Eco24I GRGCYC 3 cut(s) 262, 555, 1450
Eco47I GGWCC 7 cut(s) 829, 934, 1319, 2922, 3003, 3205, 3645
Eco47III AGCGCT 1 cut(s) 2364
Eco53kI GAGCTC 1 cut(s) 260
Eco57I CTGAAG 3 cut(s) 1254, 1526, 2975
Eco72I CACGTG 1 cut(s) 671
EcoICRI GAGCTC 1 cut(s) 260
EcoNI CCTNNNNNAGG 1 cut(s) 3333
EcoO109I RGGNCCY 3 cut(s) 829, 1446, 1447
EcoRII CCWGG 6 cut(s) 1442, 2200, 2381, 2483, 2680, 3307
EcoT14I CCWWGG 2 cut(s) 2496, 3726
EcoT22I ATGCAT 1 cut(s) 3043
EcoT38I GRGCYC 3 cut(s) 262, 555, 1450
ErhI CCWWGG 2 cut(s) 2496, 3726
Esp3I CGTCTC 2 cut(s) 2168, 3475
FaqI GGGAC 7 cut(s) 18, 306, 719, 868, 888, 1881, 2979
FauI CCCGC 1 cut(s) 38
FauNDI CATATG 1 cut(s) 557
FbaI TGATCA 3 cut(s) 382, 2041, 3804
FblI GTMKAC 2 cut(s) 2096, 2664
FokI GGATG 7 cut(s) 604, 954, 1238, 1502, 1620, 1769, 2633
FriOI GRGCYC 3 cut(s) 262, 555, 1450
FspBI CTAG 8 cut(s) 773, 1062, 1374, 1584, 1647, 1745, 2351, 3393
GlaI GCGC 4 cut(s) 2104, 2364, 3378, 3380
GsaI CCCAGC 1 cut(s) 606
GsuI CTGGAG 2 cut(s) 2049, 2404
HaeII RGCGCY 1 cut(s) 2366
HapII CCGG 3 cut(s) 2402, 2796, 3203
HgaI GACGC 4 cut(s) 1369, 3155, 3183, 3609
HhaI GCGC 4 cut(s) 2105, 2365, 3379, 3381
Hin1I GRCGYC 1 cut(s) 3175
Hin6I GCGC 4 cut(s) 2103, 2363, 3377, 3379
HinP1I GCGC 4 cut(s) 2103, 2363, 3377, 3379
HincII GTYRAC 2 cut(s) 1384, 3402
HindII GTYRAC 2 cut(s) 1384, 3402
HindIII AAGCTT 3 cut(s) 647, 1014, 2929
HpaII CCGG 3 cut(s) 2402, 2796, 3203
HphI GGTGA 1 cut(s) 2551
Hpy188III TCNNGA 8 cut(s) 179, 377, 653, 1197, 1322, 2066, 2456, 3751
HpyCH4III ACNGT 8 cut(s) 659, 691, 1071, 2566, 2970, 3469, 3668, 3774
HpyCH4IV ACGT 6 cut(s) 77, 670, 2161, 2473, 3088, 3475
HpyF10VI GCNNNNNNNGC 7 cut(s) 279, 473, 1013, 1422, 1617, 2549, 3374
HpySE526I ACGT 6 cut(s) 77, 670, 2161, 2473, 3088, 3475
Hsp92I GRCGYC 1 cut(s) 3175
HspAI GCGC 4 cut(s) 2103, 2363, 3377, 3379
KpnI GGTACC 2 cut(s) 663, 2853
Ksp22I TGATCA 3 cut(s) 382, 2041, 3804
LguI GCTCTTC 2 cut(s) 378, 501
LmnI GCTCC 8 cut(s) 453, 1034, 2118, 2173, 2385, 2701, 3439, 3730
LweI GCATC 9 cut(s) 992, 1106, 1480, 2134, 2614, 3158, 3259, 3355, 3426
MabI ACCWGGT 1 cut(s) 3307
MaeI CTAG 8 cut(s) 773, 1062, 1374, 1584, 1647, 1745, 2351, 3393
MaeII ACGT 6 cut(s) 77, 670, 2161, 2473, 3088, 3475
MaeIII GTNAC 7 cut(s) 172, 215, 488, 673, 1310, 2191, 2501
MfeI CAATTG 2 cut(s) 283, 1179
MflI RGATCY 3 cut(s) 52, 2323, 2647
MhlI GDGCHC 4 cut(s) 262, 555, 1165, 1450
MlsI TGGCCA 3 cut(s) 342, 1442, 2495
MluNI TGGCCA 3 cut(s) 342, 1442, 2495
MlyI GAGTC 7 cut(s) 169, 776, 870, 879, 1611, 1811, 2580
MmeI TCCRAC 4 cut(s) 30, 1172, 1661, 3151
Mox20I TGGCCA 3 cut(s) 342, 1442, 2495
Mph1103I ATGCAT 1 cut(s) 3043
MscI TGGCCA 3 cut(s) 342, 1442, 2495
MslI CAYNNNNRTG 5 cut(s) 300, 1208, 2142, 2658, 3267
Msp20I TGGCCA 3 cut(s) 342, 1442, 2495
MspA1I CMGCKG 1 cut(s) 1946
MspI CCGG 3 cut(s) 2402, 2796, 3203
MspR9I CCNGG 6 cut(s) 1444, 2202, 2383, 2485, 2682, 3309
MunI CAATTG 2 cut(s) 283, 1179
Mva1269I GAATGC 3 cut(s) 331, 776, 1780
MvaI CCWGG 6 cut(s) 1444, 2202, 2383, 2485, 2682, 3309
MvnI CGCG 3 cut(s) 420, 2103, 3379
MwoI GCNNNNNNNGC 7 cut(s) 279, 473, 1013, 1422, 1617, 2549, 3374
NdeI CATATG 1 cut(s) 557
NmeAIII GCCGAG 1 cut(s) 3517
NmuCI GTSAC 4 cut(s) 172, 215, 1310, 2191
NsiI ATGCAT 1 cut(s) 3043
NspI RCATGY 4 cut(s) 912, 2228, 2671, 3434
NspV TTCGAA 1 cut(s) 2183
OliI CACNNNNGTG 1 cut(s) 1208
PauI GCGCGC 1 cut(s) 3377
PceI AGGCCT 1 cut(s) 2520
PciI ACATGT 1 cut(s) 2224
PciSI GCTCTTC 2 cut(s) 378, 501
PctI GAATGC 3 cut(s) 331, 776, 1780
PfeI GAWTC 4 cut(s) 146, 1333, 3703, 3847
PflFI GACNNNGTC 1 cut(s) 963
PflMI CCANNNNNTGG 3 cut(s) 2617, 2760, 3444
Ple19I CGATCG 1 cut(s) 2421
PleI GAGTC 7 cut(s) 169, 776, 870, 879, 1610, 1810, 2579
PmaCI CACGTG 1 cut(s) 671
PmlI CACGTG 1 cut(s) 671
PpsI GAGTC 7 cut(s) 169, 776, 870, 879, 1610, 1810, 2579
Ppu21I YACGTR 1 cut(s) 671
PpuMI RGGWCCY 1 cut(s) 829
PscI ACATGT 1 cut(s) 2224
PshAI GACNNNNGTC 1 cut(s) 1317
PshBI ATTAAT 1 cut(s) 1518
PsiI TTATAA 3 cut(s) 1722, 2478, 2918
Psp124BI GAGCTC 1 cut(s) 262
Psp1406I AACGTT 1 cut(s) 2473
Psp5II RGGWCCY 1 cut(s) 829
Psp6I CCWGG 6 cut(s) 1442, 2200, 2381, 2483, 2680, 3307
PspCI CACGTG 1 cut(s) 671
PspFI CCCAGC 1 cut(s) 602
PspGI CCWGG 6 cut(s) 1442, 2200, 2381, 2483, 2680, 3307
PspOMI GGGCCC 1 cut(s) 1446
PspPPI RGGWCCY 1 cut(s) 829
PstI CTGCAG 1 cut(s) 257
PsuI RGATCY 3 cut(s) 52, 2323, 2647
PsyI GACNNNGTC 1 cut(s) 963
PteI GCGCGC 1 cut(s) 3377
PvuI CGATCG 1 cut(s) 2421
RsaI GTAC 8 cut(s) 90, 661, 921, 1084, 2206, 2438, 2450, 2851
RsaNI GTAC 8 cut(s) 89, 660, 920, 1083, 2205, 2437, 2449, 2850
RseI CAYNNNNRTG 5 cut(s) 300, 1208, 2142, 2658, 3267
SacI GAGCTC 1 cut(s) 262
SapI GCTCTTC 2 cut(s) 378, 501
ScaI AGTACT 1 cut(s) 1084
SchI GAGTC 7 cut(s) 169, 776, 870, 879, 1611, 1811, 2580
ScrFI CCNGG 6 cut(s) 1444, 2202, 2383, 2485, 2682, 3309
SduI GDGCHC 4 cut(s) 262, 555, 1165, 1450
SexAI ACCWGGT 1 cut(s) 3307
SfaNI GCATC 9 cut(s) 992, 1106, 1480, 2134, 2614, 3158, 3259, 3355, 3426
SfcI CTRYAG 3 cut(s) 192, 253, 1986
SfuI TTCGAA 1 cut(s) 2183
SinI GGWCC 7 cut(s) 829, 934, 1319, 2922, 3003, 3205, 3645
SmiMI CAYNNNNRTG 5 cut(s) 300, 1208, 2142, 2658, 3267
SmlI CTYRAG 4 cut(s) 2740, 3098, 3296, 3823
SmoI CTYRAG 4 cut(s) 2740, 3098, 3296, 3823
SseBI AGGCCT 1 cut(s) 2520
SsiI CCGC 6 cut(s) 31, 917, 1944, 2121, 2147, 2347
SspI AATATT 3 cut(s) 1169, 2978, 3562
SspMI CTAG 8 cut(s) 773, 1062, 1374, 1584, 1647, 1745, 2351, 3393
SstI GAGCTC 1 cut(s) 262
StuI AGGCCT 1 cut(s) 2520
StyD4I CCNGG 6 cut(s) 1442, 2200, 2381, 2483, 2680, 3307
StyI CCWWGG 2 cut(s) 2496, 3726
TaaI ACNGT 8 cut(s) 659, 691, 1071, 2566, 2970, 3469, 3668, 3774
TaiI ACGT 6 cut(s) 80, 673, 2164, 2476, 3091, 3478
TaqI TCGA 6 cut(s) 780, 1522, 1663, 2183, 3160, 3479
TaqII GACCGA 1 cut(s) 2431
TatI WGTACW 3 cut(s) 1082, 2436, 2448
TauI GCSGC 2 cut(s) 2150, 2350
TfiI GAWTC 4 cut(s) 146, 1333, 3703, 3847
TscAI CASTG 1 cut(s) 168
TseFI GTSAC 4 cut(s) 172, 215, 1310, 2191
Tsp45I GTSAC 4 cut(s) 172, 215, 1310, 2191
TspGWI ACGGA 1 cut(s) 2160
TspRI CASTG 1 cut(s) 168
Tth111I GACNNNGTC 1 cut(s) 963
Van91I CCANNNNNTGG 3 cut(s) 2617, 2760, 3444
VneI GTGCAC 1 cut(s) 1161
VpaK11BI GGWCC 7 cut(s) 829, 934, 1319, 2922, 3003, 3205, 3645
VspI ATTAAT 1 cut(s) 1518
XagI CCTNNNNNAGG 1 cut(s) 3333
XapI RAATTY 5 cut(s) 16, 209, 813, 1795, 1881
XceI RCATGY 4 cut(s) 912, 2228, 2671, 3434
XcmI CCANNNNNNNNNTGG 2 cut(s) 945, 1479
XmiI GTMKAC 2 cut(s) 2096, 2664
XspI CTAG 8 cut(s) 773, 1062, 1374, 1584, 1647, 1745, 2351, 3393
ZrmI AGTACT 1 cut(s) 1084
Zsp2I ATGCAT 1 cut(s) 3043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.