RchiOBHm_Chr1g0361951

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000071
Physical Location & Seq
Reverse (-)
53712524 .. 53716810
4287 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58680

Sequence Viewer

Length: 3237 bp
ATGAAGAACACCCGGTCTCCGGGGTCTGTCATGTTAGTTGAAGGTCAGAGTTCTAAGACCAATGAGGTCCAGAGGGTTGAAGTCTCTGTCAAGAACTCAGAAGGTGGTTCTTTTGGGGGTACCAAACTCACTGGTGCTAATTTCCGAACATGGAAGAAGATTATGTCTGTTTATCTTCGTGGGATACACAAAATGGGGCATGTGACTGGAACAATCAAGGCTCCTAATGAAGATAATGTGGAGGCCGATGCTAAGTGGGAAGATGATGATGGGTTTGTCATGTCAATTCTATTTCGAGCCATGACTGATGATGTGCTACAAATGGTAGAGGAGTGTGAAACTGCTGAGGCGATATGGAAGACGTTGGGAGATCTCTATACAAATGAGTCTGATTTTATACAGGTTCATGAATTGATGTGCAAAGCTGCTAATATGCAACAGAATAGGCAACCAGTAGCTGTGTATTTCACTAAGCTGAAGAATGTATGGGCTGAAATTGATCAGAAGCGTCCTTGCAAGATCAAGAATCAGGAAGATCTTGTGTGGTACCAGAAAGAGAAGGAGCTAGAACGGGTTCATGTGTTCTTGAGAGGACTTGATGAGAAGCATAGTAGTGCCAAGGGAGAATTGCTCAGAATGCTAGACCCTCCTAGTTTGAACACAGCTTTTACATACATCCGCAAGGATGAGTCTCAACAGGAAAGTGTCAAACATGCACAGGTTGAAGTATCTAGCCTAGCCATTCAGGCCAAGTCATCGGCTCCTTTCCTTCAGCAGCAGAATTCAGCCCCACAAACCACGTGTCCACAAACACCAGAACAGAATGGAGTGTCCGAACGGAAAAACCGTCATTTACTTGATATGGCTCGTGCCCTTCTTTTTAGTGCTCATATGCCTAAGTATCTTTGGGGCGATGCTATACATGCTTCCTCTCATCTTATCAATCGTCTTCCATCTAGTGTCCTTCAGGGCAAAATTCCTTTTGAGGTTCTCGCCTCTCATGTCTCCTTACCATCTTTTCACAATCTTCCAGCTCGTGTTTTTTGTTGTGTTGCTTTTGTCCATGTACCAAAAAATCAGAGGTCTAAGTTAGATGCCCGGGCCCTTAAGTGTGTGTTTGTTGGCTACGGAGGTCATCAGAAGGGGTACAAGTGTTATCATCCACCCACCAGAAAGTATTATGTCACTATGGATGTTACTTTCTTTGAAGACATGAGTTATTTCTCCTCTTCAGATACAGCTCTTCAGGGGGAGAATTCATATTTTGAAGAGCTATATCATGGAGAGGGGGAGGAATCAGAGGGAGAAGGAGAAGGCACACAGCCAGGAGGTATTTTGACGGATCCGGTTGAAACTATTAGCTCGCCTCCCCCAGCAGCAGAAGCACCAGTTTCCATCACTCAGAATGAGGTTGAAGACACAACTGCCCCTCCTGCCATCGCTTCTACCCCTGACCCACAGCTTCCTGGTATTGAAGATCATCCATTTGAGGTATGTCCATCCACTGATACTAGTAGTAGTGAGTCTAATGTTGAGCAATATGTGTTACCACATAGGACCACTCGAGGTCAATCAGCCAAAAGATATGAACCTACTCTTACTGCCAAATCAACATATCCAGTAGCCAACTATATGTCCACTAGGAGGTTGTCTAAGTCATATGCATCATTTGTGAATCAAATATCTGCTGTATCAGTACCTAACAAAGTGCAGGATGCATTGGGGGATCCAAAGTGGAGGAAGGCTATGGATGAAGAGATGGAGGCGTTGCAGAAGAACAGTACTTGGCAACTTGTGCCTCCACCACAATGCAAGAAGGCTGTAGGCTGTCGTTGGGTGTTTACCGTGAAGCATAATGCAGATGGATCAGTGAATCGATACAAAGCACGTCTTGTAGCAAAGGGATTTACTCAGACGTATGGTATAGACTATGATGAAACTTTTGCTCCTGTTGCCAAGATGAATACTATTCGGGTTCTGCTCTCGTTCGCTGCTAGTTTAAACTGGCCACTCCGACAGTTTGATGTCAAGAATGCATTTCTTCATGGAGAGTTAGCCGAGGAAGTATACATGTGCCTCCCACCAGGGTATGTAGTTGCTTCTCCTGGTGAGTTTGTATGCAAATTGAGAAAGTCTCTGTATGGTCTCAAACAGTCACCTCGTGCTTGGTTTGGAAGATTTTCACAGTTCATGCGGAAGGTTGGTTACAGGCAGAGCAACTCAGACCATACCTTGTTTCTCAAGCATCAACAGGGGAAGGTAACAGCTCTAATTATCTATGTGGATGATATGGTAATCACTGGTAATGACACTGTTGAGATGGATAGACTGCAGAGACAGCTAGCTTTTGAGTTTGAGATGAAGGACTTGGGTGAGCTTAAGTACTTCTTAGGAATTGAGGTAGCCAAGGGGAGAGAAGGAATCTATCTGTGCCAGAGGAAGTATGTTCTTGACTTGCTGACAGAGACAGGTTTATTAGATTGCAGACCTATTGATACTCCTATTGAGCAGAACCATGGTTTAGCTGAGTATCCAGATCAGGTACCTACTGATCGAGCTCGCTATCAGAGGTTAGTTGGGCGCTTAATTTATTTGGCTCATACCAGACCTGACGTTGCGTATGCAGTGAGCGTGGTGAGTCAGTTCATGCATAATCCGAGTGAGAGTCACATGGATGCTGTTATGCGAATTCTGAAGTACTTGAAGTCAGCTCCAGGTAGGGGAGTACTATTTTCTAAACACAACAACATTCTTGAGGTTTGTGGCTTCACAGATGCAGATTGGGCTGGAAATATCACAGACAGGAGGTCGACATCAGGTTACTTTACCTTTGTGGGAGGTAATTTGGTTACATGGAAGAGTAAGAAACAGAAAGTTGTGGCACGTTCTAGTGTTGAGGCGGAGTATAGAGGTATGGCTCACGGAGTGTGTGAATTGTTGTGGCTGAGAAATCTGTTACGTGATCTAGGTTTCATACTCAAAAATTCCATGCAGTTGTATTGTGACAACAAGGCAGCTATTGACATATCACAGAATCCAGTACAGCATGATCGTACTAAGCATGTGGAGGTTGATCGTCACTTTATAAAGGAGAAGCTAGATGCCAAAATCATTAGCTTTTCTTTTGTTCCAACTGAAGAGCAACTTGCAGATGTACTTACCAAAGGAGTTTCCAGGAAGGCGTTTTATGACTCACTAAGCAAGTTGGGCATGGTTGATGTATATGCGCCAACTTGA

Protein Analysis

1078

Amino Acids

121.52

Weight (kDa)

7.36

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 65 - 165 1.6e-08 gag-polypeptide of LTR copia-type
SH3_retrovirus PF25597 350 - 411 1.1e-24 Retroviral polymerase SH3-like domain
RVT_2 PF07727 593 - 836 4.8e-74 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3086
Acc65I GGTACC 3 cut(s) 119, 546, 2542
AccB1I GGYRCC 3 cut(s) 119, 546, 2542
AccI GTMKAC 2 cut(s) 2067, 2810
AciI CCGC 3 cut(s) 679, 2194, 2900
AclWI GGATC 5 cut(s) 1337, 1350, 1721, 1734, 1873
AcoI YGGCCR 1 cut(s) 2006
AcsI RAATTY 5 cut(s) 781, 975, 1255, 2688, 2983
AcuI CTGAAG 7 cut(s) 497, 755, 950, 1215, 1229, 2714, 3156
AcvI CACGTG 1 cut(s) 801
AdeI CACNNNGTG 2 cut(s) 2162, 2926
AfiI CCNNNNNNNGG 3 cut(s) 19, 1644, 2720
AflII CTTAAG 2 cut(s) 1106, 2378
AflIII ACRYGT 2 cut(s) 800, 2070
AhdI GACNNNNNGTC 1 cut(s) 2806
AhlI ACTAGT 1 cut(s) 1511
AjiI CACGTC 1 cut(s) 1889
AjnI CCWGG 6 cut(s) 1324, 1465, 2083, 2104, 2713, 3173
Alw21I GWGCWC 2 cut(s) 889, 2560
Alw26I GTCTC 8 cut(s) 21, 88, 696, 1009, 2139, 2150, 2329, 2459
AlwI GGATC 5 cut(s) 1337, 1350, 1721, 1734, 1873
AlwNI CAGNNNCTG 1 cut(s) 458
Ama87I CYCGRG 2 cut(s) 1098, 1563
AoxI GGCC 4 cut(s) 243, 747, 1101, 2006
ApaI GGGCCC 1 cut(s) 1105
ApeKI GCWGC 5 cut(s) 425, 775, 1376, 1991, 3014
ApoI RAATTY 5 cut(s) 781, 975, 1255, 2688, 2983
ArsI GACNNNNNNTTYG 2 cut(s) 787, 819
Asp700I GAANNNNTTC 2 cut(s) 573, 2179
Asp718I GGTACC 3 cut(s) 119, 546, 2542
AspLEI GCGC 2 cut(s) 2583, 3229
AspS9I GGNCC 4 cut(s) 67, 1101, 1102, 1557
AsuC2I CCSGG 4 cut(s) 13, 21, 1099, 1100
AsuHPI GGTGA 4 cut(s) 2120, 2148, 2384, 2647
AsuNHI GCTAGC 1 cut(s) 2341
AvaI CYCGRG 2 cut(s) 1098, 1563
AvaII GGWCC 2 cut(s) 67, 1557
BaeGI GKGCMC 2 cut(s) 874, 1105
BaeI ACNNNNGTAYC 2 cut(s) 1138, 1171
BalI TGGCCA 1 cut(s) 2008
BamHI GGATCC 2 cut(s) 1342, 1726
BanI GGYRCC 3 cut(s) 119, 546, 2542
BanII GRGCYC 2 cut(s) 1105, 2560
BarI GAAGNNNNNNTAC 2 cut(s) 2189, 2221
BauI CACGAG 3 cut(s) 867, 1035, 2160
BbrPI CACGTG 1 cut(s) 801
BbsI GAAGAC 4 cut(s) 365, 941, 1215, 1422
Bbv12I GWGCWC 2 cut(s) 889, 2560
BbvCI CCTCAGC 1 cut(s) 345
BbvI GCAGC 5 cut(s) 412, 787, 1388, 1978, 3026
BccI CCATC 9 cut(s) 263, 961, 1021, 1403, 1445, 1507, 1753, 1856, 2314
BciT130I CCWGG 6 cut(s) 1326, 1467, 2085, 2106, 2715, 3175
BciVI GTATCC 2 cut(s) 177, 2541
BclI TGATCA 1 cut(s) 499
BcnI CCSGG 4 cut(s) 13, 21, 1099, 1100
BcoDI GTCTC 8 cut(s) 21, 88, 696, 1009, 2139, 2150, 2329, 2459
BcuI ACTAGT 1 cut(s) 1511
BfmI CTRYAG 2 cut(s) 1821, 2330
BfoI RGCGCY 1 cut(s) 2584
BfrI CTTAAG 2 cut(s) 1106, 2378
BfuI GTATCC 2 cut(s) 177, 2541
BglI GCCNNNNNGGC 1 cut(s) 746
BglII AGATCT 2 cut(s) 370, 535
BisI GCNGC 5 cut(s) 426, 776, 1377, 1992, 3015
BlsI GCNGC 5 cut(s) 427, 777, 1378, 1993, 3016
BmcAI AGTACT 4 cut(s) 1783, 2384, 2699, 2727
Bme18I GGWCC 2 cut(s) 67, 1557
BmeRI GACNNNNNGTC 1 cut(s) 2806
BmeT110I CYCGRG 2 cut(s) 1098, 1563
BmgBI CACGTC 1 cut(s) 1889
BmgT120I GGNCC 4 cut(s) 67, 1101, 1102, 1557
BmiI GGNNCC 8 cut(s) 121, 222, 548, 762, 1103, 1344, 1728, 2544
BmsI GCATC 9 cut(s) 238, 904, 1084, 1673, 1705, 2254, 2665, 2764, 3091
BmtI GCTAGC 1 cut(s) 2345
BpiI GAAGAC 4 cut(s) 365, 941, 1215, 1422
BplI GAGNNNNNCTC 4 cut(s) 615, 647, 2119, 2151
BpmI CTGGAG 1 cut(s) 2697
Bpu10I CCTNAGC 1 cut(s) 345
BpuEI CTTGAG 3 cut(s) 607, 2225, 2774
BpuMI CCSGG 4 cut(s) 13, 21, 1099, 1100
Bsa29I ATCGAT 1 cut(s) 1876
BsaAI YACGTR 2 cut(s) 801, 2960
BsaI GGTCTC 2 cut(s) 21, 2150
BsaJI CCNNGG 7 cut(s) 20, 618, 1098, 2058, 2084, 2406, 2515
BsaWI WCCGGW 1 cut(s) 1345
BsaXI ACNNNNNCTCC 5 cut(s) 31, 1414, 1444, 1930, 1960
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 1644, 2720
Bse1I ACTGG 8 cut(s) 136, 211, 452, 1388, 1619, 2009, 2305, 3038
BseBI CCWGG 6 cut(s) 1326, 1467, 2085, 2106, 2715, 3175
BseCI ATCGAT 1 cut(s) 1876
BseDI CCNNGG 7 cut(s) 20, 618, 1098, 2058, 2084, 2406, 2515
BseLI CCNNNNNNNGG 3 cut(s) 19, 1644, 2720
BseMII CTCAG 8 cut(s) 111, 336, 646, 1415, 1925, 2235, 2517, 2936
BseNI ACTGG 8 cut(s) 136, 211, 452, 1388, 1619, 2009, 2305, 3038
BseRI GAGGAG 2 cut(s) 344, 1216
BseSI GKGCMC 2 cut(s) 874, 1105
BseXI GCAGC 5 cut(s) 412, 787, 1388, 1978, 3026
BseYI CCCAGC 1 cut(s) 1372
BsgI GTGCAG 1 cut(s) 1730
BshFI GGCC 4 cut(s) 245, 749, 1103, 2008
BshNI GGYRCC 3 cut(s) 119, 546, 2542
BshVI ATCGAT 1 cut(s) 1876
BsiHKAI GWGCWC 2 cut(s) 889, 2560
BsiHKCI CYCGRG 2 cut(s) 1098, 1563
BsiSI CCGG 4 cut(s) 13, 20, 1099, 1346
BslI CCNNNNNNNGG 3 cut(s) 19, 1644, 2720
BsmAI GTCTC 8 cut(s) 21, 88, 696, 1009, 2139, 2150, 2329, 2459
BsmI GAATGC 2 cut(s) 642, 2038
BsnI GGCC 4 cut(s) 245, 749, 1103, 2008
Bso31I GGTCTC 2 cut(s) 21, 2150
BsoBI CYCGRG 2 cut(s) 1098, 1563
Bsp120I GGGCCC 1 cut(s) 1101
Bsp1286I GDGCHC 4 cut(s) 874, 889, 1105, 2560
Bsp19I CCATGG 1 cut(s) 2515
BspACI CCGC 3 cut(s) 679, 2194, 2900
BspANI GGCC 4 cut(s) 245, 749, 1103, 2008
BspCNI CTCAG 8 cut(s) 110, 337, 645, 1414, 1924, 2234, 2518, 2937
BspDI ATCGAT 1 cut(s) 1876
BspHI TCATGA 1 cut(s) 406
BspLI GGNNCC 8 cut(s) 121, 222, 548, 762, 1103, 1344, 1728, 2544
BspMAI CTGCAG 1 cut(s) 2334
BspOI GCTAGC 1 cut(s) 2345
BspPI GGATC 5 cut(s) 1337, 1350, 1721, 1734, 1873
BspQI GCTCTTC 3 cut(s) 1248, 1263, 3132
BspT107I GGYRCC 3 cut(s) 119, 546, 2542
BspTI CTTAAG 2 cut(s) 1106, 2378
BspTNI GGTCTC 2 cut(s) 21, 2150
BsrI ACTGG 8 cut(s) 136, 211, 452, 1388, 1619, 2009, 2305, 3038
BssECI CCNNGG 7 cut(s) 20, 618, 1098, 2058, 2084, 2406, 2515
BssNAI GTATAC 1 cut(s) 2068
BssSI CACGAG 3 cut(s) 867, 1035, 2160
BssT1I CCWWGG 3 cut(s) 618, 2406, 2515
Bst1107I GTATAC 1 cut(s) 2068
Bst2BI CACGAG 3 cut(s) 867, 1035, 2160
Bst2UI CCWGG 6 cut(s) 1326, 1467, 2085, 2106, 2715, 3175
Bst4CI ACNGT 7 cut(s) 848, 1781, 1846, 2019, 2154, 2187, 2314
Bst6I CTCTTC 6 cut(s) 1234, 1248, 1263, 1749, 2852, 3132
BstAFI CTTAAG 2 cut(s) 1106, 2378
BstAPI GCANNNNNTGC 1 cut(s) 1795
BstBAI YACGTR 2 cut(s) 801, 2960
BstC8I GCNNGC 3 cut(s) 1364, 2343, 2560
BstDSI CCRYGG 1 cut(s) 2515
BstH2I RGCGCY 1 cut(s) 2584
BstHHI GCGC 2 cut(s) 2583, 3229
BstMAI GTCTC 8 cut(s) 21, 88, 696, 1009, 2139, 2150, 2329, 2459
BstNI CCWGG 6 cut(s) 1326, 1467, 2085, 2106, 2715, 3175
BstNSI RCATGY 5 cut(s) 203, 716, 926, 2074, 3065
BstSFI CTRYAG 2 cut(s) 1821, 2330
BstSLI GKGCMC 2 cut(s) 874, 1105
BstV1I GCAGC 5 cut(s) 412, 787, 1388, 1978, 3026
BstV2I GAAGAC 4 cut(s) 365, 941, 1215, 1422
BstX2I RGATCY 4 cut(s) 370, 535, 1342, 1726
BstYI RGATCY 4 cut(s) 370, 535, 1342, 1726
BstZ17I GTATAC 1 cut(s) 2068
Bsu15I ATCGAT 1 cut(s) 1876
BsuI GTATCC 2 cut(s) 177, 2541
BsuRI GGCC 4 cut(s) 245, 749, 1103, 2008
BsuTUI ATCGAT 1 cut(s) 1876
BtgI CCRYGG 1 cut(s) 2515
BtgZI GCGATG 2 cut(s) 927, 1423
BtrI CACGTC 1 cut(s) 1889
BtsI GCAGTG 1 cut(s) 2631
BtsIMutI CAGTG 6 cut(s) 129, 1503, 1875, 2298, 2310, 2631
Cac8I GCNNGC 3 cut(s) 1364, 2343, 2560
CaiI CAGNNNCTG 1 cut(s) 458
CciI TCATGA 1 cut(s) 406
CfoI GCGC 2 cut(s) 2583, 3229
Cfr13I GGNCC 4 cut(s) 67, 1101, 1102, 1557
Cfr9I CCCGGG 1 cut(s) 1098
ClaI ATCGAT 1 cut(s) 1876
CseI GACGC 1 cut(s) 497
DraI TTTAAA 1 cut(s) 2001
DraIII CACNNNGTG 2 cut(s) 2162, 2926
DriI GACNNNNNGTC 1 cut(s) 2806
EaeI YGGCCR 1 cut(s) 2006
Eam1104I CTCTTC 6 cut(s) 1234, 1248, 1263, 1749, 2852, 3132
Eam1105I GACNNNNNGTC 1 cut(s) 2806
EarI CTCTTC 6 cut(s) 1234, 1248, 1263, 1749, 2852, 3132
EciI GGCGGA 1 cut(s) 2915
Ecl136II GAGCTC 1 cut(s) 2558
Eco130I CCWWGG 3 cut(s) 618, 2406, 2515
Eco24I GRGCYC 2 cut(s) 1105, 2560
Eco31I GGTCTC 2 cut(s) 21, 2150
Eco47I GGWCC 2 cut(s) 67, 1557
Eco53kI GAGCTC 1 cut(s) 2558
Eco57I CTGAAG 7 cut(s) 497, 755, 950, 1215, 1229, 2714, 3156
Eco72I CACGTG 1 cut(s) 801
Eco88I CYCGRG 2 cut(s) 1098, 1563
EcoICRI GAGCTC 1 cut(s) 2558
EcoO109I RGGNCCY 1 cut(s) 1102
EcoRI GAATTC 3 cut(s) 781, 1255, 2688
EcoRII CCWGG 6 cut(s) 1324, 1465, 2083, 2104, 2713, 3173
EcoT14I CCWWGG 3 cut(s) 618, 2406, 2515
EcoT22I ATGCAT 4 cut(s) 1666, 1720, 2038, 2652
EcoT38I GRGCYC 2 cut(s) 1105, 2560
ErhI CCWWGG 3 cut(s) 618, 2406, 2515
FalI AAGNNNNNCTT 6 cut(s) 1875, 1907, 2372, 2404, 3129, 3161
FauNDI CATATG 2 cut(s) 891, 1660
FbaI TGATCA 1 cut(s) 499
FblI GTMKAC 2 cut(s) 2067, 2810
Fnu4HI GCNGC 5 cut(s) 426, 776, 1377, 1992, 3015
FriOI GRGCYC 2 cut(s) 1105, 2560
Fsp4HI GCNGC 5 cut(s) 426, 776, 1377, 1992, 3015
GlaI GCGC 2 cut(s) 2582, 3228
GluI GCNGC 5 cut(s) 426, 776, 1377, 1992, 3015
GsaI CCCAGC 1 cut(s) 1376
GsuI CTGGAG 1 cut(s) 2697
HaeII RGCGCY 1 cut(s) 2584
HaeIII GGCC 4 cut(s) 245, 749, 1103, 2008
HapII CCGG 4 cut(s) 13, 20, 1099, 1346
HgaI GACGC 1 cut(s) 497
HhaI GCGC 2 cut(s) 2583, 3229
Hin6I GCGC 2 cut(s) 2581, 3227
HinP1I GCGC 2 cut(s) 2581, 3227
HincII GTYRAC 1 cut(s) 2811
HindII GTYRAC 1 cut(s) 2811
HpaII CCGG 4 cut(s) 13, 20, 1099, 1346
HphI GGTGA 4 cut(s) 2120, 2148, 2384, 2647
Hpy166II GTNNAC 5 cut(s) 806, 1638, 1842, 2068, 2811
Hpy8I GTNNAC 5 cut(s) 806, 1638, 1842, 2068, 2811
HpyCH4III ACNGT 7 cut(s) 848, 1781, 1846, 2019, 2154, 2187, 2314
HpyCH4IV ACGT 7 cut(s) 362, 800, 1888, 1916, 2613, 2884, 2959
HpySE526I ACGT 7 cut(s) 362, 800, 1888, 1916, 2613, 2884, 2959
HspAI GCGC 2 cut(s) 2581, 3227
KpnI GGTACC 3 cut(s) 123, 550, 2546
Ksp22I TGATCA 1 cut(s) 499
LguI GCTCTTC 3 cut(s) 1248, 1263, 3132
LmnI GCTCC 5 cut(s) 226, 562, 766, 1951, 2716
Lsp1109I GCAGC 5 cut(s) 412, 787, 1388, 1978, 3026
LweI GCATC 9 cut(s) 238, 904, 1084, 1673, 1705, 2254, 2665, 2764, 3091
MaeII ACGT 7 cut(s) 362, 800, 1888, 1916, 2613, 2884, 2959
MflI RGATCY 4 cut(s) 370, 535, 1342, 1726
MhlI GDGCHC 4 cut(s) 874, 889, 1105, 2560
MlsI TGGCCA 1 cut(s) 2008
MluNI TGGCCA 1 cut(s) 2008
MlyI GAGTC 6 cut(s) 395, 698, 1532, 2647, 2674, 3185
MmeI TCCRAC 2 cut(s) 2038, 3155
Mox20I TGGCCA 1 cut(s) 2008
Mph1103I ATGCAT 4 cut(s) 1666, 1720, 2038, 2652
MroXI GAANNNNTTC 2 cut(s) 573, 2179
MscI TGGCCA 1 cut(s) 2008
MseI TTAA 4 cut(s) 1107, 2000, 2379, 2585
MslI CAYNNNNRTG 3 cut(s) 612, 1807, 2673
Msp20I TGGCCA 1 cut(s) 2008
MspCI CTTAAG 2 cut(s) 1106, 2378
MspI CCGG 4 cut(s) 13, 20, 1099, 1346
MssI GTTTAAAC 1 cut(s) 2001
Mva1269I GAATGC 2 cut(s) 642, 2038
MvaI CCWGG 6 cut(s) 1326, 1467, 2085, 2106, 2715, 3175
NciI CCSGG 4 cut(s) 13, 21, 1099, 1100
NcoI CCATGG 1 cut(s) 2515
NdeI CATATG 2 cut(s) 891, 1660
NheI GCTAGC 1 cut(s) 2341
NlaIV GGNNCC 8 cut(s) 121, 222, 548, 762, 1103, 1344, 1728, 2544
NmeAIII GCCGAG 1 cut(s) 2083
NmuCI GTSAC 6 cut(s) 202, 1183, 2154, 2666, 3002, 3077
NsiI ATGCAT 4 cut(s) 1666, 1720, 2038, 2652
NspI RCATGY 5 cut(s) 203, 716, 926, 2074, 3065
PaeR7I CTCGAG 1 cut(s) 1563
PagI TCATGA 1 cut(s) 406
PciI ACATGT 1 cut(s) 2070
PciSI GCTCTTC 3 cut(s) 1248, 1263, 3132
PcsI WCGNNNNNNNCGW 1 cut(s) 844
PctI GAATGC 2 cut(s) 642, 2038
PdmI GAANNNNTTC 2 cut(s) 573, 2179
PfeI GAWTC 6 cut(s) 526, 1295, 1675, 1873, 2421, 3034
PfoI TCCNGGA 1 cut(s) 3173
PkrI GCNGC 5 cut(s) 427, 777, 1378, 1993, 3016
PleI GAGTC 6 cut(s) 394, 697, 1531, 2646, 2673, 3185
PmaCI CACGTG 1 cut(s) 801
PmeI GTTTAAAC 1 cut(s) 2001
PmlI CACGTG 1 cut(s) 801
PpsI GAGTC 6 cut(s) 394, 697, 1531, 2646, 2673, 3185
Ppu21I YACGTR 2 cut(s) 801, 2960
PscI ACATGT 1 cut(s) 2070
PsiI TTATAA 1 cut(s) 3086
Psp124BI GAGCTC 1 cut(s) 2560
Psp6I CCWGG 6 cut(s) 1324, 1465, 2083, 2104, 2713, 3173
PspCI CACGTG 1 cut(s) 801
PspFI CCCAGC 1 cut(s) 1372
PspGI CCWGG 6 cut(s) 1324, 1465, 2083, 2104, 2713, 3173
PspN4I GGNNCC 8 cut(s) 121, 222, 548, 762, 1103, 1344, 1728, 2544
PspOMI GGGCCC 1 cut(s) 1101
PspPI GGNCC 4 cut(s) 67, 1101, 1102, 1557
PspXI VCTCGAGB 1 cut(s) 1563
PstI CTGCAG 1 cut(s) 2334
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 4 cut(s) 370, 535, 1342, 1726
RseI CAYNNNNRTG 3 cut(s) 612, 1807, 2673
SacI GAGCTC 1 cut(s) 2560
SalI GTCGAC 1 cut(s) 2809
SapI GCTCTTC 3 cut(s) 1248, 1263, 3132
SaqAI TTAA 4 cut(s) 1107, 2000, 2379, 2585
SatI GCNGC 5 cut(s) 426, 776, 1377, 1992, 3015
Sau96I GGNCC 4 cut(s) 67, 1101, 1102, 1557
ScaI AGTACT 4 cut(s) 1783, 2384, 2699, 2727
SchI GAGTC 6 cut(s) 395, 698, 1532, 2647, 2674, 3185
SduI GDGCHC 4 cut(s) 874, 889, 1105, 2560
SfaNI GCATC 9 cut(s) 238, 904, 1084, 1673, 1705, 2254, 2665, 2764, 3091
SfcI CTRYAG 2 cut(s) 1821, 2330
Sfr274I CTCGAG 1 cut(s) 1563
SinI GGWCC 2 cut(s) 67, 1557
SlaI CTCGAG 1 cut(s) 1563
SmaI CCCGGG 1 cut(s) 1100
SmiMI CAYNNNNRTG 3 cut(s) 612, 1807, 2673
SmlI CTYRAG 6 cut(s) 586, 1106, 1563, 2240, 2378, 2753
SmoI CTYRAG 6 cut(s) 586, 1106, 1563, 2240, 2378, 2753
SpeI ACTAGT 1 cut(s) 1511
SrfI GCCCGGGC 1 cut(s) 1100
SsiI CCGC 3 cut(s) 679, 2194, 2900
SstI GAGCTC 1 cut(s) 2560
StyI CCWWGG 3 cut(s) 618, 2406, 2515
TaaI ACNGT 7 cut(s) 848, 1781, 1846, 2019, 2154, 2187, 2314
TaiI ACGT 7 cut(s) 365, 803, 1891, 1919, 2616, 2887, 2962
TaqI TCGA 5 cut(s) 295, 1564, 1876, 2554, 2810
TatI WGTACW 6 cut(s) 1781, 2382, 2697, 2725, 3040, 3154
TfiI GAWTC 6 cut(s) 526, 1295, 1675, 1873, 2421, 3034
Tru1I TTAA 4 cut(s) 1107, 2000, 2379, 2585
Tru9I TTAA 4 cut(s) 1107, 2000, 2379, 2585
TscAI CASTG 6 cut(s) 136, 1510, 1875, 2305, 2317, 2631
TseFI GTSAC 6 cut(s) 202, 1183, 2154, 2666, 3002, 3077
TseI GCWGC 5 cut(s) 425, 775, 1376, 1991, 3014
Tsp45I GTSAC 6 cut(s) 202, 1183, 2154, 2666, 3002, 3077
TspGWI ACGGA 4 cut(s) 853, 1143, 1355, 2937
TspMI CCCGGG 1 cut(s) 1098
TspRI CASTG 6 cut(s) 136, 1510, 1875, 2305, 2317, 2631
Vha464I CTTAAG 2 cut(s) 1106, 2378
VpaK11BI GGWCC 2 cut(s) 67, 1557
XapI RAATTY 5 cut(s) 781, 975, 1255, 2688, 2983
XceI RCATGY 5 cut(s) 203, 716, 926, 2074, 3065
XhoI CTCGAG 1 cut(s) 1563
XmaI CCCGGG 1 cut(s) 1098
XmiI GTMKAC 2 cut(s) 2067, 2810
XmnI GAANNNNTTC 2 cut(s) 573, 2179
ZrmI AGTACT 4 cut(s) 1783, 2384, 2699, 2727
Zsp2I ATGCAT 4 cut(s) 1666, 1720, 2038, 2652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.