FvH4_6g44611

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000071
Physical Location & Seq
Reverse (-)
34357130 .. 34359825
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44611.t1

Sequence Viewer

Length: 2529 bp
ATGTGCATTTTCATCGGTTATCCTGTTGGTCAGAAAGCCTATAAGCTTTACGATCTCTCCAGTCATAAAGTTTTTACAAGTCGTGATGTGATCTTTCATGAAAATATTTTCCCTTATCAGTCTGTTGTGCCTGAGTCCAAATCTGGTCCAATTCTTCCTTTGGCCCAAATCACAAGCCCACCTCACACATCATTACAATCCAGTCCACTAGATCAACCTCAACATTCAACACAGATCCCGTCTCCTTCCGCCACCGATTTCCCAGCGACGCCCAGTCCTGTCGTCGCATCACCGGCGCCATCCCTTTCCGGCGCTGTCTCGTCGCCTCCGCCTCCGCCTCCGCCTCCGCTACCACAACCCCGTCGATCCTTGCGCCTTCAAGCTCTCTCTCACCAGAACGACTCCTCTTCGTCTCTTCCGGCCTGTCTTCGGTCCGACCCCATCTCAGGTCCGACCTCATCTCCGGAACAAGCTCCGGCGGTGTCGCTTCCTTCCCCATCCCACTCAGCTCCACAAGCTCCTCAACCCAATTTCGGTCGCCCTACTCCACCTTTGTATCCTGATCAGTCGACTTCATCCTCTCTGAGTCGTCTTCCTCTCATCCCTCAATCAGTCCGACCACCGTCCAGTCCAACCAATTCGTTGCCATCCGGCCTCGAACCATCTCCTCCAGTCCAACCTAATACCCACATCGTTTCCTATCCAAACCCGCCGCCTAACATAGACCATCCACCTCCGCCTCTCACAGACCAGCCACCTACCCACTTGCCGGACCCGCAGCCTCTCCGTCGCTCTGCTCGGCAAACGACGCCTTCCGTTCGGTTGCGTGATTTTGTGTGTTCACATGTGACATCGAGCTTCCCAAACTCATTGTCTTCTTCCTCTCCAGGTCCTGTCAAAATCAAAGGTACGAGATATCCTCTGTGCAATTATGTGTCATATCATCGTTATACTCCTGCGCATGCCTCTTTTATTGCTCAGATATCCCAGGCCAGTGAACCACGCACTTATGCCGAAGCTGCACAACATCCAGAATGGCAAGAAGCGATGCATTCTGAATTGCAGGCATTGGAGGATAATAACACTTGGACTCTCACCACTTTACCGGTTGGCAAGACTCCTATTGATTGTCGGTGGGTTTACAAGATCAAGCATCGATCAGACGGTTCTATTGAGAGGTACAAAGCTCGTTTGGTTGCCAAGGGATTCACTCAAATGGAAGGTATCGATTATCATGACACTTTCTCACCCACTGCCAAAATTATTTCTGTTCGATGCTTATTATCTTTGGCGGCTGCTCAAAATTGGTCCATTCATCAGTTGGATGTCAATAATGCCTTTTTGCATGGTGATCTTAATGAGGAAATTTTCATGTCTCCTCCTCCTGGTCTTCGGCGACAGGGGGAGCATTTGGTTTGTCGCCTTAATAAGTCTTTGTATGGTTTAAAACAAGCATCCCGGCAATGGTTTGCCAAATTTTCAGATGCTATTCGATCAGCTGGCTATATTCAGTCCAAGGCAGATTACTCATTATTCACTCGAAAGCAAGGGAAGTCATTCACAACTTTATTGATATATGTTGATGATATATTAATTACTAGTAATGATCCGGTTGCTATTTCTGCTTTAAAGAATTTTCTGAATAGCCGGTTTCGTATTAAGGATCTTGGTGATTTAAAGTATTTTCTTGGCATAGAGGTTTCTCGTTCCAAAAGTGGAATCTATATTTCTCAGCGCAAGTATGCATTGGAGATCATCAGTGATGCAGGTTTATTAGGAGCAGCTCCTGTAGATACTCCTATGGAGCGCGGTTCGAAATTATCAAATAAGGGAGATCTTCTTAAGGATGCTTCACGTTATAGAAGATTGGTTGGTAGGTTGATCTATCTCACCATCTCAAGGCCTGATATAACCTACTCTGTGCATGTATTGAGCAGGTTCATGCATGAGCCTCGCAAGCCTCATATGGAAGCAGCTTTACGAGTTGTTCGTTATCTCAAGAGCAATCCAGGTCAAGGTCTATTTTTCTCCTCACAAAATGATTTCAGACTTCGTGCTTTTTGTGATTCGGATTGGGCTGGTTGTCCCATGACCCGAAGATCTACTACAGGTTATTGTGTCTTTTTTGGTTCTTCACTAATTTCTTGGAGGTCAAAGAGACAAAAGACAGTTTCATTGTCTTCAGCTGAAGCAGAATATCGAGCAATGACAGGAGCTTGTTGTGAGTTAACTTGGTTAAAAAATCTTCTCAAAGATCTAAATATTCTTCATTCAAAACCTGCTTTGTTGTATTGTGACAATAGAGCAGCTTTGCATATTGCTGCCAATCCTGTTTTTCATGAAAGAACTAGACACATTGAGATGGATTGTCACTTCATCAGAGACAAGATTCAAGATGGGTCAGTTGTTACTAAGTTCGTCAGTTCTGGAAATCAGTTAGGTGATGTTCTTACCAAAGCATTGGGAAAGGAAGTTTTCATCCCTATGATTCGCAAGTTGGGAGTTCGAGATATTCACTCTCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_3 Retrotrans_gag

Protein Analysis

843

Amino Acids

93.29

Weight (kDa)

9.45

Isoelectric Point (pI)

63.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SH3_retrovirus PF25597 2 - 42 4.9e-15 Retroviral polymerase SH3-like domain
RVT_2 PF07727 360 - 602 1.5e-73 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 960
Acc36I ACCTGC 3 cut(s) 1757, 1926, 2287
AccB1I GGYRCC 1 cut(s) 295
AccI GTMKAC 1 cut(s) 569
AccII CGCG 1 cut(s) 1809
AccIII TCCGGA 1 cut(s) 463
AclWI GGATC 4 cut(s) 229, 360, 1601, 1671
AcsI RAATTY 3 cut(s) 1365, 1475, 1633
AcuI CTGAAG 2 cut(s) 2166, 2208
AcyI GRCGYC 3 cut(s) 269, 296, 809
AfaI GTAC 2 cut(s) 910, 1181
AfiI CCNNNNNNNGG 8 cut(s) 429, 446, 533, 769, 1385, 1464, 1899, 2015
AflII CTTAAG 1 cut(s) 1841
AflIII ACRYGT 1 cut(s) 844
AgeI ACCGGT 1 cut(s) 1105
AgsI TTSAA 4 cut(s) 228, 380, 2274, 2393
AhdI GACNNNNNGTC 1 cut(s) 273
AhlI ACTAGT 1 cut(s) 1598
AjnI CCWGG 4 cut(s) 886, 987, 1384, 2008
Alw26I GTCTC 6 cut(s) 246, 322, 417, 1380, 2152, 2376
AlwI GGATC 4 cut(s) 229, 360, 1601, 1671
AlwNI CAGNNNCTG 2 cut(s) 893, 1787
Aor13HI TCCGGA 1 cut(s) 463
AoxI GGCC 5 cut(s) 162, 420, 652, 990, 1901
ApeKI GCWGC 7 cut(s) 778, 1019, 1295, 1781, 1973, 2306, 2321
ApoI RAATTY 3 cut(s) 1365, 1475, 1633
ArsI GACNNNNNNTTYG 2 cut(s) 857, 889
AseI ATTAAT 1 cut(s) 1592
AsiGI ACCGGT 1 cut(s) 1105
Asp700I GAANNNNTTC 1 cut(s) 1556
AspLEI GCGC 6 cut(s) 298, 314, 375, 961, 1737, 1809
AspS9I GGNCC 7 cut(s) 146, 163, 432, 449, 772, 890, 1308
AsuC2I CCSGG 1 cut(s) 1459
AsuHPI GGTGA 8 cut(s) 282, 383, 1087, 1239, 1361, 1682, 1882, 2453
AsuII TTCGAA 1 cut(s) 1814
AvaII GGWCC 6 cut(s) 146, 432, 449, 772, 890, 1308
BaeI ACNNNNGTAYC 2 cut(s) 539, 572
BanI GGYRCC 1 cut(s) 295
BarI GAAGNNNNNNTAC 4 cut(s) 1962, 1994, 2089, 2121
BbsI GAAGAC 5 cut(s) 419, 584, 867, 1382, 2172
BbvI GCAGC 7 cut(s) 790, 1006, 1282, 1793, 1985, 2308, 2318
BccI CCATC 9 cut(s) 307, 449, 505, 655, 670, 735, 1901, 2356, 2390
BcgI CGANNNNNNTGC 3 cut(s) 29, 1934, 1968
BciT130I CCWGG 4 cut(s) 888, 989, 1386, 2010
BciVI GTATCC 1 cut(s) 567
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 1 cut(s) 1459
BcoDI GTCTC 6 cut(s) 246, 322, 417, 1380, 2152, 2376
BcuI ACTAGT 1 cut(s) 1598
BfaI CTAG 3 cut(s) 209, 1599, 2349
BfmI CTRYAG 2 cut(s) 1788, 2106
BfoI RGCGCY 2 cut(s) 299, 315
BfrI CTTAAG 1 cut(s) 1841
BfuAI ACCTGC 3 cut(s) 1757, 1926, 2287
BfuI GTATCC 1 cut(s) 567
BglII AGATCT 3 cut(s) 1834, 2099, 2254
BisI GCNGC 9 cut(s) 713, 779, 1020, 1293, 1296, 1782, 1974, 2307, 2322
BlsI GCNGC 9 cut(s) 714, 780, 1021, 1294, 1297, 1783, 1975, 2308, 2323
Bme1390I CCNGG 5 cut(s) 888, 989, 1386, 1459, 2010
Bme18I GGWCC 6 cut(s) 146, 432, 449, 772, 890, 1308
BmeRI GACNNNNNGTC 1 cut(s) 273
BmgT120I GGNCC 7 cut(s) 146, 163, 432, 449, 772, 890, 1308
BmiI GGNNCC 2 cut(s) 297, 774
BmrFI CCNGG 5 cut(s) 888, 989, 1386, 1459, 2010
BmrI ACTGGG 1 cut(s) 267
BmsI GCATC 8 cut(s) 296, 1038, 1162, 1265, 1463, 1474, 1753, 1837
BmuI ACTGGG 1 cut(s) 267
BoxI GACNNNNGTC 1 cut(s) 622
BpiI GAAGAC 5 cut(s) 419, 584, 867, 1382, 2172
BplI GAGNNNNNCTC 2 cut(s) 904, 936
BpmI CTGGAG 3 cut(s) 43, 654, 870
Bpu14I TTCGAA 1 cut(s) 1814
BpuEI CTTGAG 2 cut(s) 1882, 1982
BpuMI CCSGG 1 cut(s) 1459
Bsa29I ATCGAT 2 cut(s) 1156, 1227
BsaHI GRCGYC 3 cut(s) 269, 296, 809
BsaJI CCNNGG 3 cut(s) 987, 1200, 1515
BsaWI WCCGGW 3 cut(s) 463, 1105, 1609
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 445, 475
Bsc4I CCNNNNNNNGG 8 cut(s) 429, 446, 533, 769, 1385, 1464, 1899, 2015
Bse118I RCCGGY 3 cut(s) 292, 1105, 1647
Bse1I ACTGG 6 cut(s) 60, 201, 273, 627, 671, 993
Bse3DI GCAATG 2 cut(s) 1469, 2211
BseAI TCCGGA 1 cut(s) 463
BseBI CCWGG 4 cut(s) 888, 989, 1386, 2010
BseCI ATCGAT 2 cut(s) 1156, 1227
BseDI CCNNGG 3 cut(s) 987, 1200, 1515
BseLI CCNNNNNNNGG 8 cut(s) 429, 446, 533, 769, 1385, 1464, 1899, 2015
BseMI GCAATG 2 cut(s) 1469, 2211
BseMII CTCAG 6 cut(s) 123, 459, 519, 575, 992, 1745
BseNI ACTGG 6 cut(s) 60, 201, 273, 627, 671, 993
BseRI GAGGAG 6 cut(s) 394, 510, 657, 1368, 1371, 2020
BseXI GCAGC 7 cut(s) 790, 1006, 1282, 1793, 1985, 2308, 2318
BseYI CCCAGC 1 cut(s) 262
BsgI GTGCAG 1 cut(s) 1005
Bsh1236I CGCG 1 cut(s) 1809
Bsh1285I CGRYCG 1 cut(s) 538
BshFI GGCC 5 cut(s) 164, 422, 654, 992, 1903
BshNI GGYRCC 1 cut(s) 295
BshTI ACCGGT 1 cut(s) 1105
BshVI ATCGAT 2 cut(s) 1156, 1227
BsiEI CGRYCG 1 cut(s) 538
BslFI GGGAC 1 cut(s) 2070
BslI CCNNNNNNNGG 8 cut(s) 429, 446, 533, 769, 1385, 1464, 1899, 2015
BsmAI GTCTC 6 cut(s) 246, 322, 417, 1380, 2152, 2376
BsmBI CGTCTC 2 cut(s) 246, 417
BsmFI GGGAC 1 cut(s) 2070
BsmI GAATGC 1 cut(s) 1051
BsnI GGCC 5 cut(s) 164, 422, 654, 992, 1903
Bsp119I TTCGAA 1 cut(s) 1814
Bsp13I TCCGGA 1 cut(s) 463
BspANI GGCC 5 cut(s) 164, 422, 654, 992, 1903
BspCNI CTCAG 6 cut(s) 124, 458, 518, 576, 991, 1744
BspDI ATCGAT 2 cut(s) 1156, 1227
BspEI TCCGGA 1 cut(s) 463
BspFNI CGCG 1 cut(s) 1809
BspHI TCATGA 3 cut(s) 97, 1234, 2338
BspLI GGNNCC 2 cut(s) 297, 774
BspMI ACCTGC 3 cut(s) 1757, 1926, 2287
BspPI GGATC 4 cut(s) 229, 360, 1601, 1671
BspT104I TTCGAA 1 cut(s) 1814
BspT107I GGYRCC 1 cut(s) 295
BspTI CTTAAG 1 cut(s) 1841
BsrDI GCAATG 2 cut(s) 1469, 2211
BsrFI RCCGGY 3 cut(s) 292, 1105, 1647
BsrI ACTGG 6 cut(s) 60, 201, 273, 627, 671, 993
BssAI RCCGGY 3 cut(s) 292, 1105, 1647
BssECI CCNNGG 3 cut(s) 987, 1200, 1515
BssNI GRCGYC 3 cut(s) 269, 296, 809
BssT1I CCWWGG 2 cut(s) 1200, 1515
Bst2UI CCWGG 4 cut(s) 888, 989, 1386, 2010
Bst4CI ACNGT 3 cut(s) 624, 1166, 2170
Bst6I CTCTTC 2 cut(s) 412, 420
BstACI GRCGYC 3 cut(s) 269, 296, 809
BstAFI CTTAAG 1 cut(s) 1841
BstBI TTCGAA 1 cut(s) 1814
BstC8I GCNNGC 4 cut(s) 963, 1065, 1501, 1958
BstDEI CTNAG 7 cut(s) 132, 445, 505, 584, 978, 1731, 2412
BstFNI CGCG 1 cut(s) 1809
BstH2I RGCGCY 2 cut(s) 299, 315
BstHHI GCGC 6 cut(s) 298, 314, 375, 961, 1737, 1809
BstMAI GTCTC 6 cut(s) 246, 322, 417, 1380, 2152, 2376
BstMCI CGRYCG 1 cut(s) 538
BstMWI GCNNNNNNNGC 7 cut(s) 293, 515, 775, 808, 1019, 1622, 1957
BstNI CCWGG 4 cut(s) 888, 989, 1386, 2010
BstNSI RCATGY 3 cut(s) 848, 965, 1928
BstPAI GACNNNNGTC 1 cut(s) 622
BstSCI CCNGG 5 cut(s) 886, 987, 1384, 1457, 2008
BstSFI CTRYAG 2 cut(s) 1788, 2106
BstUI CGCG 1 cut(s) 1809
BstV1I GCAGC 7 cut(s) 790, 1006, 1282, 1793, 1985, 2308, 2318
BstV2I GAAGAC 5 cut(s) 419, 584, 867, 1382, 2172
BstX2I RGATCY 5 cut(s) 234, 1663, 1834, 2099, 2254
BstXI CCANNNNNNTGG 1 cut(s) 2461
BstYI RGATCY 5 cut(s) 234, 1663, 1834, 2099, 2254
Bsu15I ATCGAT 2 cut(s) 1156, 1227
BsuI GTATCC 1 cut(s) 567
BsuRI GGCC 5 cut(s) 164, 422, 654, 992, 1903
BsuTUI ATCGAT 2 cut(s) 1156, 1227
BtgZI GCGATG 1 cut(s) 1061
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 3 cut(s) 1000, 1251, 1765
BveI ACCTGC 3 cut(s) 1757, 1926, 2287
Cac8I GCNNGC 4 cut(s) 963, 1065, 1501, 1958
CaiI CAGNNNCTG 2 cut(s) 893, 1787
CciI TCATGA 3 cut(s) 97, 1234, 2338
CfoI GCGC 6 cut(s) 298, 314, 375, 961, 1737, 1809
Cfr10I RCCGGY 3 cut(s) 292, 1105, 1647
Cfr13I GGNCC 7 cut(s) 146, 163, 432, 449, 772, 890, 1308
ClaI ATCGAT 2 cut(s) 1156, 1227
CpoI CGGWCCG 1 cut(s) 432
CseI GACGC 2 cut(s) 277, 817
Csp6I GTAC 2 cut(s) 909, 1180
CspAI ACCGGT 1 cut(s) 1105
CspI CGGWCCG 1 cut(s) 432
CviQI GTAC 2 cut(s) 909, 1180
DdeI CTNAG 7 cut(s) 132, 445, 505, 584, 978, 1731, 2412
DinI GGCGCC 1 cut(s) 297
DraI TTTAAA 3 cut(s) 1446, 1629, 1677
DriI GACNNNNNGTC 1 cut(s) 273
Eam1104I CTCTTC 2 cut(s) 412, 420
Eam1105I GACNNNNNGTC 1 cut(s) 273
EarI CTCTTC 2 cut(s) 412, 420
EciI GGCGGA 5 cut(s) 238, 318, 324, 330, 726
Eco130I CCWWGG 2 cut(s) 1200, 1515
Eco147I AGGCCT 1 cut(s) 1903
Eco32I GATATC 2 cut(s) 917, 984
Eco47I GGWCC 6 cut(s) 146, 432, 449, 772, 890, 1308
Eco57I CTGAAG 2 cut(s) 2166, 2208
EcoO109I RGGNCCY 1 cut(s) 890
EcoRII CCWGG 4 cut(s) 886, 987, 1384, 2008
EcoRV GATATC 2 cut(s) 917, 984
EcoT14I CCWWGG 2 cut(s) 1200, 1515
EcoT22I ATGCAT 3 cut(s) 1053, 1747, 1947
EgeI GGCGCC 1 cut(s) 297
EheI GGCGCC 1 cut(s) 297
ErhI CCWWGG 2 cut(s) 1200, 1515
Esp3I CGTCTC 2 cut(s) 246, 417
FaqI GGGAC 1 cut(s) 2070
FauI CCCGC 2 cut(s) 717, 783
FauNDI CATATG 1 cut(s) 1965
FbaI TGATCA 1 cut(s) 562
FblI GTMKAC 1 cut(s) 569
Fnu4HI GCNGC 9 cut(s) 713, 779, 1020, 1293, 1296, 1782, 1974, 2307, 2322
Fsp4HI GCNGC 9 cut(s) 713, 779, 1020, 1293, 1296, 1782, 1974, 2307, 2322
FspBI CTAG 3 cut(s) 209, 1599, 2349
FspI TGCGCA 1 cut(s) 960
GlaI GCGC 6 cut(s) 297, 313, 374, 960, 1736, 1808
GluI GCNGC 9 cut(s) 713, 779, 1020, 1293, 1296, 1782, 1974, 2307, 2322
GsaI CCCAGC 1 cut(s) 266
GsuI CTGGAG 3 cut(s) 43, 654, 870
HaeII RGCGCY 2 cut(s) 299, 315
HaeIII GGCC 5 cut(s) 164, 422, 654, 992, 1903
HgaI GACGC 2 cut(s) 277, 817
HhaI GCGC 6 cut(s) 298, 314, 375, 961, 1737, 1809
Hin1I GRCGYC 3 cut(s) 269, 296, 809
Hin6I GCGC 6 cut(s) 296, 312, 373, 959, 1735, 1807
HinP1I GCGC 6 cut(s) 296, 312, 373, 959, 1735, 1807
HincII GTYRAC 2 cut(s) 570, 2229
HindII GTYRAC 2 cut(s) 570, 2229
HindIII AAGCTT 1 cut(s) 44
HpaI GTTAAC 1 cut(s) 2229
HphI GGTGA 8 cut(s) 282, 383, 1087, 1239, 1361, 1682, 1882, 2453
Hpy166II GTNNAC 6 cut(s) 206, 570, 842, 998, 1141, 2229
Hpy8I GTNNAC 6 cut(s) 206, 570, 842, 998, 1141, 2229
Hpy99I CGWCG 6 cut(s) 271, 287, 325, 366, 792, 811
HpyAV CCTTC 5 cut(s) 255, 386, 501, 822, 1214
HpyCH4III ACNGT 3 cut(s) 624, 1166, 2170
HpyCH4IV ACGT 1 cut(s) 1855
HpyF10VI GCNNNNNNNGC 7 cut(s) 293, 515, 775, 808, 1019, 1622, 1957
HpyF3I CTNAG 7 cut(s) 132, 445, 505, 584, 978, 1731, 2412
HpySE526I ACGT 1 cut(s) 1855
Hsp92I GRCGYC 3 cut(s) 269, 296, 809
HspAI GCGC 6 cut(s) 296, 312, 373, 959, 1735, 1807
KasI GGCGCC 1 cut(s) 295
Kpn2I TCCGGA 1 cut(s) 463
Ksp22I TGATCA 1 cut(s) 562
KspAI GTTAAC 1 cut(s) 2229
LmnI GCTCC 8 cut(s) 478, 514, 523, 1405, 1778, 1789, 1804, 2213
Lsp1109I GCAGC 7 cut(s) 790, 1006, 1282, 1793, 1985, 2308, 2318
LweI GCATC 8 cut(s) 296, 1038, 1162, 1265, 1463, 1474, 1753, 1837
MaeI CTAG 3 cut(s) 209, 1599, 2349
MaeII ACGT 1 cut(s) 1855
MaeIII GTNAC 4 cut(s) 847, 2294, 2369, 2407
MflI RGATCY 5 cut(s) 234, 1663, 1834, 2099, 2254
Mly113I GGCGCC 1 cut(s) 296
MlyI GAGTC 5 cut(s) 143, 395, 595, 1084, 1111
MmeI TCCRAC 6 cut(s) 459, 476, 640, 656, 700, 1302
Mph1103I ATGCAT 3 cut(s) 1053, 1747, 1947
MroI TCCGGA 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 1556
MslI CAYNNNNRTG 3 cut(s) 1214, 2360, 2483
MspA1I CMGCKG 2 cut(s) 1499, 2186
MspCI CTTAAG 1 cut(s) 1841
MspR9I CCNGG 5 cut(s) 888, 989, 1386, 1459, 2010
Mva1269I GAATGC 1 cut(s) 1051
MvaI CCWGG 4 cut(s) 888, 989, 1386, 2010
MvnI CGCG 1 cut(s) 1809
MwoI GCNNNNNNNGC 7 cut(s) 293, 515, 775, 808, 1019, 1622, 1957
NarI GGCGCC 1 cut(s) 296
NciI CCSGG 1 cut(s) 1459
NdeI CATATG 1 cut(s) 1965
NlaIV GGNNCC 2 cut(s) 297, 774
NmeAIII GCCGAG 1 cut(s) 778
NmuCI GTSAC 3 cut(s) 847, 2294, 2369
NsbI TGCGCA 1 cut(s) 960
NsiI ATGCAT 3 cut(s) 1053, 1747, 1947
NspI RCATGY 3 cut(s) 848, 965, 1928
NspV TTCGAA 1 cut(s) 1814
PaeI GCATGC 1 cut(s) 965
PagI TCATGA 3 cut(s) 97, 1234, 2338
PceI AGGCCT 1 cut(s) 1903
PciI ACATGT 1 cut(s) 844
PcsI WCGNNNNNNNCGW 1 cut(s) 1987
PctI GAATGC 1 cut(s) 1051
PdmI GAANNNNTTC 1 cut(s) 1556
PfeI GAWTC 5 cut(s) 1206, 1719, 2066, 2389, 2488
PinAI ACCGGT 1 cut(s) 1105
PkrI GCNGC 9 cut(s) 714, 780, 1021, 1294, 1297, 1783, 1975, 2308, 2323
PleI GAGTC 5 cut(s) 142, 395, 594, 1084, 1111
PluTI GGCGCC 1 cut(s) 299
PpsI GAGTC 5 cut(s) 142, 395, 594, 1084, 1111
PpuMI RGGWCCY 1 cut(s) 890
PscI ACATGT 1 cut(s) 844
PshAI GACNNNNGTC 1 cut(s) 622
PshBI ATTAAT 1 cut(s) 1592
Psp5II RGGWCCY 1 cut(s) 890
Psp6I CCWGG 4 cut(s) 886, 987, 1384, 2008
PspFI CCCAGC 1 cut(s) 262
PspGI CCWGG 4 cut(s) 886, 987, 1384, 2008
PspN4I GGNNCC 2 cut(s) 297, 774
PspPI GGNCC 7 cut(s) 146, 163, 432, 449, 772, 890, 1308
PspPPI RGGWCCY 1 cut(s) 890
PstNI CAGNNNCTG 2 cut(s) 893, 1787
PsuI RGATCY 5 cut(s) 234, 1663, 1834, 2099, 2254
PvuII CAGCTG 2 cut(s) 1499, 2186
RsaI GTAC 2 cut(s) 910, 1181
RsaNI GTAC 2 cut(s) 909, 1180
RseI CAYNNNNRTG 3 cut(s) 1214, 2360, 2483
Rsr2I CGGWCCG 1 cut(s) 432
RsrII CGGWCCG 1 cut(s) 432
SalI GTCGAC 1 cut(s) 568
SatI GCNGC 9 cut(s) 713, 779, 1020, 1293, 1296, 1782, 1974, 2307, 2322
Sau96I GGNCC 7 cut(s) 146, 163, 432, 449, 772, 890, 1308
SchI GAGTC 5 cut(s) 143, 395, 595, 1084, 1111
ScrFI CCNGG 5 cut(s) 888, 989, 1386, 1459, 2010
SfaNI GCATC 8 cut(s) 296, 1038, 1162, 1265, 1463, 1474, 1753, 1837
SfcI CTRYAG 2 cut(s) 1788, 2106
SfoI GGCGCC 1 cut(s) 297
SfuI TTCGAA 1 cut(s) 1814
SgrAI CRCCGGYG 1 cut(s) 292
SinI GGWCC 6 cut(s) 146, 432, 449, 772, 890, 1308
SmiMI CAYNNNNRTG 3 cut(s) 1214, 2360, 2483
SmlI CTYRAG 3 cut(s) 1841, 1897, 1997
SmoI CTYRAG 3 cut(s) 1841, 1897, 1997
SpeI ACTAGT 1 cut(s) 1598
SphI GCATGC 1 cut(s) 965
SseBI AGGCCT 1 cut(s) 1903
SspDI GGCGCC 1 cut(s) 295
SspI AATATT 2 cut(s) 106, 2263
SspMI CTAG 3 cut(s) 209, 1599, 2349
StuI AGGCCT 1 cut(s) 1903
StyD4I CCNGG 5 cut(s) 886, 987, 1384, 1457, 2008
StyI CCWWGG 2 cut(s) 1200, 1515
TaaI ACNGT 3 cut(s) 624, 1166, 2170
TaiI ACGT 1 cut(s) 1858
TaqII GACCGA 2 cut(s) 420, 524
TauI GCSGC 2 cut(s) 715, 1295
TfiI GAWTC 5 cut(s) 1206, 1719, 2066, 2389, 2488
TscAI CASTG 3 cut(s) 1000, 1258, 1765
TseFI GTSAC 3 cut(s) 847, 2294, 2369
TseI GCWGC 7 cut(s) 778, 1019, 1295, 1781, 1973, 2306, 2321
Tsp45I GTSAC 3 cut(s) 847, 2294, 2369
TspGWI ACGGA 2 cut(s) 776, 805
TspRI CASTG 3 cut(s) 1000, 1258, 1765
Vha464I CTTAAG 1 cut(s) 1841
VpaK11BI GGWCC 6 cut(s) 146, 432, 449, 772, 890, 1308
VspI ATTAAT 1 cut(s) 1592
XapI RAATTY 3 cut(s) 1365, 1475, 1633
XceI RCATGY 3 cut(s) 848, 965, 1928
XcmI CCANNNNNNNNNTGG 1 cut(s) 1318
XmiI GTMKAC 1 cut(s) 569
XmnI GAANNNNTTC 1 cut(s) 1556
XspI CTAG 3 cut(s) 209, 1599, 2349
Zsp2I ATGCAT 3 cut(s) 1053, 1747, 1947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.