MD12G1150100.v1.1

Retrotransposon gag protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000071
Physical Location & Seq
Forward (+)
23034943 .. 23042247
7305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1150100.v1.1.491

Sequence Viewer

Length: 4311 bp
ATGGCCGACGACATTCGTTTCTTCGAGCTGAACACCGGTGCTAAGATCCCGTCGATAGGGTTGGGGACCTGGCAGTCCAGCCCTGGCGTCGTCGGCGACGCCGTTCACGCCGCCGTCAAGACTGACAAACAATTTGCATCATGGCAAAGATGCAATGATATGGTTCTTGCCTGGATTCTCAACTCAGTTCACGATGACATTGCGAGCAGTGTTTCTTATTACACGACAGCAACCGATGTTTGGGCTGACCTGCGAGATCGTTTCTCACAAGGAAACGACTCACGCATTTATCAAATCAAGAGAGAGATCGTTGAACACAGACAGGAACAACAATCGATCTCGGTTTACTACACGAAACTGAAAGCGTTGTGGGATGAACTGGCATCCTACAATGAAACTCCGACTTGTACCTGTGGGGGATTGAAGAAGATAAATGATCGAGATGAGAAAGAAAGGGTGATGCAGTTCCTGATGGGTTTAAACGACTCTTATGCAGCAGTACGTGGACAGATACTATTGATGCAGCCACTACCAGACACTCGGAGGGCATATTCCCTTGTTCTCCAACAAGAGAAGCAAGTAGAGGTATCTCTAAATCGTAACAATATAAATCTGCATGCTATGAATATAACCCGTAACAGGGACACTGCTGCACCGAAAGGAAACACCCTTCAATGCTCATATTGTGATCAAAAGTACCACACCGTGGATCGATGCTTTTACTTGTATGGTTTCCCGCCTGGCCATAAGTATCATGGCAAATCAGTCAAGCCACCAAACAAAAGAAAACCTGCGGCAAACCAAGTAACGGTGGAGACCGAGACCACCAAGGGTGTAGACTCACGACACAAGGCAACGTCCAGTGACGGTCCGAAGTTCACCACAGAAGAATATAATCAACTTATGGCAATGCTCAAGAAGAGCAACGTTGATGGTAATCCGCAGCATTTTGCAAATGCAACAGGTACAATCACACCTTCTTCTGACTTGTCAGAGAAAACTTTATATTGGATCATTGATAGTGGGGCAACAGACCATGTATCTCCTTCTCCTCATTTGCTAGACAAGAAGTCATTATCCATGCTGGATTCTGTTCAATTACCGAATGGAGGACATGCCTTGATAGACTCGATTGGTTCTATACAAGTTACTCCACACATGAAACTTGATGGAGTGCTCCATGTGCCCAACTTTCGAGTTTACTTATTATCTCACTTAGGGCTGATAATGGTGGTGAATTTACCTCTTTACGTTCGTTTTTTTGATAACAAGGGTATCATATTCCAAACTTCTTGTCCGTCCACACCACAACAAAACGGGGTTGTTGAACGGAAACACCGTCATCTACTAAATGTTGGTCGAGCTCTACGGTTTCAAGCCCATTTACCACAAACCTTTTGGGGGGAAAGCATACAAACAGCATGTTATTTAATTAATCGTTTACCTACACCTCTTCTTAGTCATAAAACACCTTATGAGCTTTTGCATTGTCAAACTCCCGTTTTTTCGCATCTCCGGGTTTTCGGTTGTTTATGTTATGCTACCAACCTTCATCCTCACCATAAGTTTGATCAACGTGCTAAAAGATGTGTTTTTGTTGGGTACCCATTAGGTCAGAAGGGTTATCGTGTTTATGACCTTACCACACACAAAAATTTTTCCACTCGTGACGTGACCTTCCATGAAACCACGTTCCCTTTCTCCACTCAACCACTTGACCATCACCCTGATACACCTGTCCTACCTATCCCCACCGACTCCCTATCTCATCCTAATCCCCCACCGCCACCGACTGCACCTCCTTCCCCCACACCGACCGACACTTTTTTATCCACACCAACGGAAACTGCTCCTCCCATAGAACCTTCTCCTCCCACACCGACCGAACCTCACTTAGTCCCTCCACCACTCCCTACCCCCCACATGCCCCACCTGCCCCTCGCCAATCTGGCCGCGCCCTTAAGCCCTCTGTCAGGCTGCAGGGATACCATTTATATCATGTGCAATCCCTTGCTCCCAGCGCCACGTCCTCCTCCATGTCAGGCTAACGGTGATCCTCAATGGCGTGCTGCCATGGACTCTGAGCTTCTGGCTCTTGAACAAAACAACACATGGACTCTCACTACCTTACCTCCTGGTCATCGCGCAATCGGTTGCCGATGGGTCTACAAGATCAAGTACAACTCTGACGGTACCGTCGAGCGTTATAAAGCTCGTCTTGTGGCCAAAGGCTTTACTCAACGCGAAGGTATTGATTACAAGGAAACTTTTGCTCCTGTTGCCAAACTCATCACCGTCCGTTGTTTGTTAGCCGTTGCTTCTGTTCGTCATTGGTCCTTACATCAAATGGATGTCCACAATGCCTTCCTTCATGGTGACCTTTCTGAGGAAGTTTATATGCAGCTGCCTCCTGGACTTCATCGGCAGGGGGAGCATAATGTATGCCGACTCAACAAGTCCCTTTATGGCCTTAAGCAAGCCTCTCGCAGCTGGTTCCATAAATTTTCCACTGCCATAGGTCAAGCTGGCTACCAGCAATCAATGGCTGACTACTCCCTATTCACTAAGGTGCGTGGAAATTCGTTTACAGCCGTCTTGCTTTATGTTGACGACATGATTATCACAGGCAACGATGAGGCTGCCATCCATGACCTCAAGCAATTTCTTCAATCTCATTTTCGCATTAAGGACCTTGGACACTTAAAGTATTTCCTTGGTGTGGAGGTGGCTCGATCTTCTCAAGGAATCGCCATCTCACAACGTAAATATGCGCTTGACATTATTGATGAAGCCGGCTTACTTGGTGCAAAGCCTGCAAAGTTCCCAATGGAAGAAAACTTGAGATTATCTCCAACGGAAGGGCAACTTCTCCATAATGCCTCACAATACAGGAGGCTTGTTGGGAAACTTATTTATCTCACAATCACTAGGCCAGAGATTTCATACTCGGTCCATATACTCAGCCAATTTATGCAACAACCAAGGAAGCCTCATCTAGACGCAGTGCATCGTCTTCTACGATACTTGAAGGGATCACCTGGACAAGGGCTTCTTTTTCCTTCAAATGGGAGTTTAACGTTGAAAGGATATTGCGACGCCGATTGGGCTCGCTGTTCACTTACGAGAAGATCTGTCACAGGCTATTGTATATTTCTTGGAGGTGCACTAGTGTCTTGGAAAACCAAGAAACAATCTACCGTTTCGAGATCCTCAGCTGAAGCGGAGTATCGTGCCATGGCTTCAGCTACATGTGAGCTTACGTGGATGAAGTACTTATTGACTGATCTACAAATTGATCACAAGGGTCCAGCAAAGCTGCACTGCGACAACCAAGCTGCACTCCACATAGCTGCCAATCCAGTTTTTCACGAACGCACCAAACACATAGAGATTGACTGTCATGTTGTTCGTGAACGACTACGTTCTGGGGTTATTTCGACAACCTATGTACCTACTGGACAGCAGCTTGCCGATATATTCACCAAGCCACTTGGACAGTCAGCATTCCGTTCTCTACTTGGCAAGATGGGTTATCGGCATATTGATTGTGCTCAAATCTACGGCAATGAGAAGGAGATTGGTTTGGTTCTGAAGAAACTGTTTGAAGATGGCGTGGTGAAGCGCGAGGATTTGTGGATTACCTCCAAACTCTGGAATACTGATCATGCTCCAGAAGATGTACCAGAGGCATTGGACGGGACTCTGAGAGACTTGCAACTGGATTATGTTGACTTGTACCTTATACACTGGCCAATCCGGATGAAGAAGGGGTCAGGAGCAAGTTTTAACCTTGAAAACTTTATTGACCCTGATATCCCTGCGACTTGGAGAGCAATGGAAACACTCTATGATTCGGGAAAGGCTCGAGCTATTGGTGTATGCAATTTCTCCACAAAAAAGCTGTCAGATTTGCTGGATATTGCACGTGTTCCTCCTGCTGTTGACCAAGTGGAGTGCCATCCTTCGTGGCAGCAGGACAAGCTACGTTCTTTCTGTAAATCCAAGGGAGTTCACCTTTCTGTGATCCTTACGAATCCGATTCTTGCAACAGTCGCTGAGAAATTAGGCAAAAGTCCGGCACAAGTTGCTCTTCGTTGGGGACTGCAAAAGGGTCATAGTGTTCTTCCCAAGAGTACCAATGAAGCGAGGATCAAGGAGAACATTGATGTGTTTGGTTGGTCCATCCCAGATGACTTGTTCGCCAAGTTTTCTGAAATTGAGCAGGCAAGACTCATCAGATGGCTCTCACCTGTTCATCATACTTCTGGTCCGTACAGGAGTTTAGAGGAATTCTGGGACGGTGAAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs

Protein Analysis

1437

Amino Acids

161.52

Weight (kDa)

8.75

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 337 - 405 3.4e-08 Pol polyprotein, beta-barrel domain
SH3_retrovirus PF25597 510 - 570 4.4e-22 Retroviral polymerase SH3-like domain
RVT_2 PF07727 702 - 944 3.7e-77 Reverse transcriptase (RNA-dependent DNA polymerase)
Aldo_ket_red PF00248 1172 - 1339 4.4e-34 Aldo/keto reductase family
Aldo_ket_red PF00248 1344 - 1406 2.7e-07 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2208
AarI CACCTGC 1 cut(s) 1941
AasI GACNNNNNNGTC 2 cut(s) 73, 2195
Acc36I ACCTGC 3 cut(s) 258, 799, 1941
Acc65I GGTACC 2 cut(s) 1602, 2192
AccB1I GGYRCC 2 cut(s) 1602, 2192
AccB7I CCANNNNNTGG 2 cut(s) 706, 3651
AccI GTMKAC 2 cut(s) 837, 2166
AccII CGCG 4 cut(s) 1955, 2145, 2244, 3624
AccIII TCCGGA 1 cut(s) 3756
AciI CCGC 7 cut(s) 111, 737, 794, 941, 1784, 1953, 3221
AclI AACGTT 2 cut(s) 927, 3077
AclWI GGATC 8 cut(s) 40, 717, 1019, 2048, 3040, 3201, 4018, 4157
AcoI YGGCCR 5 cut(s) 3, 742, 1950, 2223, 3749
AcsI RAATTY 5 cut(s) 1237, 1654, 2501, 2578, 4289
AcuI CTGAAG 3 cut(s) 3225, 3237, 3611
AcvI CACGTG 1 cut(s) 3926
AcyI GRCGYC 3 cut(s) 87, 99, 3096
AdeI CACNNNGTG 1 cut(s) 706
AflII CTTAAG 2 cut(s) 1960, 2471
AflIII ACRYGT 2 cut(s) 3248, 3925
AgeI ACCGGT 1 cut(s) 35
AhdI GACNNNNNGTC 1 cut(s) 1069
AhlI ACTAGT 1 cut(s) 3166
AjiI CACGTC 2 cut(s) 1672, 2027
AjnI CCWGG 7 cut(s) 68, 82, 170, 739, 2134, 2410, 3037
AleI CACNNNNGTG 1 cut(s) 2567
Alw21I GWGCWC 4 cut(s) 1179, 1366, 3166, 3553
Alw26I GTCTC 3 cut(s) 809, 815, 3702
Alw44I GTGCAC 1 cut(s) 3162
AlwI GGATC 8 cut(s) 40, 717, 1019, 2048, 3040, 3201, 4018, 4157
AlwNI CAGNNNCTG 2 cut(s) 469, 4055
Ama87I CYCGRG 1 cut(s) 3864
Aor13HI TCCGGA 1 cut(s) 3756
AoxI GGCC 7 cut(s) 3, 742, 1950, 2223, 2467, 2930, 3749
ApaLI GTGCAC 1 cut(s) 3162
ApoI RAATTY 5 cut(s) 1237, 1654, 2501, 2578, 4289
ArsI GACNNNNNNTTYG 6 cut(s) 116, 148, 1279, 1311, 3887, 3919
AseI ATTAAT 1 cut(s) 1434
AsiGI ACCGGT 1 cut(s) 35
Asp718I GGTACC 2 cut(s) 1602, 2192
AspLEI GCGC 5 cut(s) 1957, 2023, 2147, 2773, 3624
AspS9I GGNCC 8 cut(s) 66, 869, 2334, 2689, 2950, 3305, 4179, 4268
AsuC2I CCSGG 1 cut(s) 1517
AvaI CYCGRG 1 cut(s) 3864
AvaII GGWCC 8 cut(s) 66, 869, 2334, 2689, 2950, 3305, 4179, 4268
BaeGI GKGCMC 2 cut(s) 1188, 3166
BaeI ACNNNNGTAYC 6 cut(s) 1258, 1291, 1722, 1755, 3432, 3465
BalI TGGCCA 3 cut(s) 744, 2225, 3751
BanI GGYRCC 2 cut(s) 1602, 2192
BanII GRGCYC 2 cut(s) 1366, 3109
BarI GAAGNNNNNNTAC 2 cut(s) 2781, 2813
BauI CACGAG 1 cut(s) 1665
BbrPI CACGTG 1 cut(s) 3926
BbsI GAAGAC 1 cut(s) 3005
Bbv12I GWGCWC 4 cut(s) 1179, 1366, 3166, 3553
BbvCI CCTCAGC 1 cut(s) 3211
BceAI ACGGC 5 cut(s) 86, 98, 2297, 2576, 3577
BcgI CGANNNNNNTGC 8 cut(s) 182, 216, 473, 507, 2465, 2499, 2621, 2655
BciT130I CCWGG 7 cut(s) 70, 84, 172, 741, 2136, 2412, 3039
BciVI GTATCC 1 cut(s) 1978
BclI TGATCA 4 cut(s) 688, 1570, 3295, 3661
BcnI CCSGG 1 cut(s) 1517
BcoDI GTCTC 3 cut(s) 809, 815, 3702
BcuI ACTAGT 1 cut(s) 3166
BfaI CTAG 5 cut(s) 1061, 2928, 2996, 3167, 4309
BfmI CTRYAG 1 cut(s) 1978
BfoI RGCGCY 1 cut(s) 2024
BfrI CTTAAG 2 cut(s) 1960, 2471
BfuAI ACCTGC 3 cut(s) 258, 799, 1941
BfuI GTATCC 1 cut(s) 1978
BglI GCCNNNNNGGC 2 cut(s) 1949, 3104
BglII AGATCT 1 cut(s) 3128
BmcAI AGTACT 1 cut(s) 3272
Bme1390I CCNGG 8 cut(s) 70, 84, 172, 741, 1517, 2136, 2412, 3039
Bme18I GGWCC 8 cut(s) 66, 869, 2334, 2689, 2950, 3305, 4179, 4268
BmeRI GACNNNNNGTC 1 cut(s) 1069
BmeT110I CYCGRG 1 cut(s) 3864
BmgBI CACGTC 2 cut(s) 1672, 2027
BmgT120I GGNCC 8 cut(s) 66, 869, 2334, 2689, 2950, 3305, 4179, 4268
BmiI GGNNCC 5 cut(s) 67, 1604, 2194, 2495, 3306
BmrFI CCNGG 8 cut(s) 70, 84, 172, 741, 1517, 2136, 2412, 3039
BmsI GCATC 8 cut(s) 140, 146, 392, 450, 510, 704, 1519, 3016
BpiI GAAGAC 1 cut(s) 3005
BplI GAGNNNNNCTC 2 cut(s) 2833, 2865
BpmI CTGGAG 1 cut(s) 3654
Bpu10I CCTNAGC 1 cut(s) 3211
BpuEI CTTGAG 4 cut(s) 899, 2639, 2724, 2860
BpuMI CCSGG 1 cut(s) 1517
Bsa29I ATCGAT 2 cut(s) 335, 712
BsaAI YACGTR 3 cut(s) 503, 3261, 3926
BsaBI GATNNNNATC 1 cut(s) 936
BsaHI GRCGYC 3 cut(s) 87, 99, 3096
BsaI GGTCTC 2 cut(s) 809, 815
BsaJI CCNNGG 9 cut(s) 82, 705, 828, 2073, 2692, 2713, 2981, 3234, 4002
BsaWI WCCGGW 2 cut(s) 35, 3756
BsaXI ACNNNNNCTCC 8 cut(s) 1783, 1813, 1837, 1867, 2116, 2146, 2257, 2287
Bse118I RCCGGY 2 cut(s) 35, 2792
Bse1I ACTGG 6 cut(s) 384, 861, 3359, 3460, 3723, 3752
Bse3DI GCAATG 5 cut(s) 160, 198, 915, 3571, 3840
Bse8I GATNNNNATC 1 cut(s) 936
BseAI TCCGGA 1 cut(s) 3756
BseBI CCWGG 7 cut(s) 70, 84, 172, 741, 2136, 2412, 3039
BseCI ATCGAT 2 cut(s) 335, 712
BseDI CCNNGG 9 cut(s) 82, 705, 828, 2073, 2692, 2713, 2981, 3234, 4002
BseJI GATNNNNATC 1 cut(s) 936
BseMI GCAATG 5 cut(s) 160, 198, 915, 3571, 3840
BseMII CTCAG 7 cut(s) 198, 2073, 2376, 2974, 3225, 3695, 4047
BseNI ACTGG 6 cut(s) 384, 861, 3359, 3460, 3723, 3752
BseRI GAGGAG 4 cut(s) 1041, 1842, 1860, 2022
BseSI GKGCMC 2 cut(s) 1188, 3166
BseYI CCCAGC 1 cut(s) 2017
BsgI GTGCAG 4 cut(s) 636, 1779, 3302, 3321
Bsh1236I CGCG 4 cut(s) 1955, 2145, 2244, 3624
Bsh1285I CGRYCG 2 cut(s) 1818, 1884
BshFI GGCC 7 cut(s) 5, 744, 1952, 2225, 2469, 2932, 3751
BshNI GGYRCC 2 cut(s) 1602, 2192
BshTI ACCGGT 1 cut(s) 35
BshVI ATCGAT 2 cut(s) 335, 712
BsiEI CGRYCG 2 cut(s) 1818, 1884
BsiHKAI GWGCWC 4 cut(s) 1179, 1366, 3166, 3553
BsiHKCI CYCGRG 1 cut(s) 3864
BsiSI CCGG 5 cut(s) 36, 1516, 2793, 3757, 4076
BslFI GGGAC 6 cut(s) 79, 656, 1883, 2443, 3712, 4113
BsmAI GTCTC 3 cut(s) 809, 815, 3702
BsmFI GGGAC 6 cut(s) 79, 656, 1883, 2443, 3712, 4113
BsmI GAATGC 1 cut(s) 3503
BsnI GGCC 7 cut(s) 5, 744, 1952, 2225, 2469, 2932, 3751
Bso31I GGTCTC 2 cut(s) 809, 815
BsoBI CYCGRG 1 cut(s) 3864
Bsp1286I GDGCHC 6 cut(s) 1179, 1188, 1366, 3109, 3166, 3553
Bsp13I TCCGGA 1 cut(s) 3756
Bsp19I CCATGG 2 cut(s) 2073, 3234
BspACI CCGC 7 cut(s) 111, 737, 794, 941, 1784, 1953, 3221
BspANI GGCC 7 cut(s) 5, 744, 1952, 2225, 2469, 2932, 3751
BspCNI CTCAG 7 cut(s) 197, 2074, 2377, 2973, 3224, 3696, 4048
BspDI ATCGAT 2 cut(s) 335, 712
BspEI TCCGGA 1 cut(s) 3756
BspFNI CGCG 4 cut(s) 1955, 2145, 2244, 3624
BspLI GGNNCC 5 cut(s) 67, 1604, 2194, 2495, 3306
BspMAI CTGCAG 1 cut(s) 1982
BspMI ACCTGC 3 cut(s) 258, 799, 1941
BspPI GGATC 8 cut(s) 40, 717, 1019, 2048, 3040, 3201, 4018, 4157
BspQI GCTCTTC 2 cut(s) 914, 4095
BspT107I GGYRCC 2 cut(s) 1602, 2192
BspTI CTTAAG 2 cut(s) 1960, 2471
BspTNI GGTCTC 2 cut(s) 809, 815
BsrDI GCAATG 5 cut(s) 160, 198, 915, 3571, 3840
BsrFI RCCGGY 2 cut(s) 35, 2792
BsrI ACTGG 6 cut(s) 384, 861, 3359, 3460, 3723, 3752
BssAI RCCGGY 2 cut(s) 35, 2792
BssECI CCNNGG 9 cut(s) 82, 705, 828, 2073, 2692, 2713, 2981, 3234, 4002
BssNI GRCGYC 3 cut(s) 87, 99, 3096
BssSI CACGAG 1 cut(s) 1665
BssT1I CCWWGG 7 cut(s) 828, 2073, 2692, 2713, 2981, 3234, 4002
Bst2BI CACGAG 1 cut(s) 1665
Bst2UI CCWGG 7 cut(s) 70, 84, 172, 741, 2136, 2412, 3039
Bst6I CTCTTC 3 cut(s) 914, 1458, 4095
BstACI GRCGYC 3 cut(s) 87, 99, 3096
BstAFI CTTAAG 2 cut(s) 1960, 2471
BstAPI GCANNNNNTGC 2 cut(s) 2813, 4085
BstBAI YACGTR 3 cut(s) 503, 3261, 3926
BstDSI CCRYGG 3 cut(s) 705, 2073, 3234
BstEII GGTNACC 1 cut(s) 2375
BstENI CCTNNNNNAGG 3 cut(s) 1971, 2384, 3042
BstFNI CGCG 4 cut(s) 1955, 2145, 2244, 3624
BstH2I RGCGCY 1 cut(s) 2024
BstHHI GCGC 5 cut(s) 1957, 2023, 2147, 2773, 3624
BstMAI GTCTC 3 cut(s) 809, 815, 3702
BstMCI CGRYCG 2 cut(s) 1818, 1884
BstNI CCWGG 7 cut(s) 70, 84, 172, 741, 2136, 2412, 3039
BstNSI RCATGY 5 cut(s) 620, 1118, 1425, 1927, 3252
BstPI GGTNACC 1 cut(s) 2375
BstSCI CCNGG 8 cut(s) 68, 82, 170, 739, 1515, 2134, 2410, 3037
BstSFI CTRYAG 1 cut(s) 1978
BstSLI GKGCMC 2 cut(s) 1188, 3166
BstUI CGCG 4 cut(s) 1955, 2145, 2244, 3624
BstV2I GAAGAC 1 cut(s) 3005
BstX2I RGATCY 3 cut(s) 45, 3128, 3206
BstYI RGATCY 3 cut(s) 45, 3128, 3206
Bsu15I ATCGAT 2 cut(s) 335, 712
BsuI GTATCC 1 cut(s) 1978
BsuRI GGCC 7 cut(s) 5, 744, 1952, 2225, 2469, 2932, 3751
BsuTUI ATCGAT 2 cut(s) 335, 712
BtgI CCRYGG 3 cut(s) 705, 2073, 3234
BtgZI GCGATG 1 cut(s) 2126
BtrI CACGTC 2 cut(s) 1672, 2027
BtsI GCAGTG 5 cut(s) 214, 645, 2508, 3009, 3319
BtsIMutI CAGTG 7 cut(s) 214, 645, 868, 2508, 3009, 3319, 3745
BveI ACCTGC 3 cut(s) 258, 799, 1941
CaiI CAGNNNCTG 2 cut(s) 469, 4055
CfoI GCGC 5 cut(s) 1957, 2023, 2147, 2773, 3624
Cfr10I RCCGGY 2 cut(s) 35, 2792
Cfr13I GGNCC 8 cut(s) 66, 869, 2334, 2689, 2950, 3305, 4179, 4268
ClaI ATCGAT 2 cut(s) 335, 712
CpoI CGGWCCG 1 cut(s) 869
CseI GACGC 4 cut(s) 76, 107, 3008, 3104
CspAI ACCGGT 1 cut(s) 35
CspI CGGWCCG 1 cut(s) 869
DraI TTTAAA 1 cut(s) 480
DraIII CACNNNGTG 1 cut(s) 706
DrdI GACNNNNNNGTC 2 cut(s) 73, 2195
DriI GACNNNNNGTC 1 cut(s) 1069
DseDI GACNNNNNNGTC 2 cut(s) 73, 2195
EaeI YGGCCR 5 cut(s) 3, 742, 1950, 2223, 3749
Eam1104I CTCTTC 3 cut(s) 914, 1458, 4095
Eam1105I GACNNNNNGTC 1 cut(s) 1069
EarI CTCTTC 3 cut(s) 914, 1458, 4095
Ecl136II GAGCTC 1 cut(s) 1364
Eco130I CCWWGG 7 cut(s) 828, 2073, 2692, 2713, 2981, 3234, 4002
Eco24I GRGCYC 2 cut(s) 1366, 3109
Eco31I GGTCTC 2 cut(s) 809, 815
Eco32I GATATC 1 cut(s) 3814
Eco47I GGWCC 8 cut(s) 66, 869, 2334, 2689, 2950, 3305, 4179, 4268
Eco53kI GAGCTC 1 cut(s) 1364
Eco57I CTGAAG 3 cut(s) 3225, 3237, 3611
Eco72I CACGTG 1 cut(s) 3926
Eco88I CYCGRG 1 cut(s) 3864
Eco91I GGTNACC 1 cut(s) 2375
EcoICRI GAGCTC 1 cut(s) 1364
EcoNI CCTNNNNNAGG 3 cut(s) 1971, 2384, 3042
EcoO109I RGGNCCY 2 cut(s) 66, 2689
EcoO65I GGTNACC 1 cut(s) 2375
EcoRI GAATTC 1 cut(s) 4289
EcoRII CCWGG 7 cut(s) 68, 82, 170, 739, 2134, 2410, 3037
EcoRV GATATC 1 cut(s) 3814
EcoT14I CCWWGG 7 cut(s) 828, 2073, 2692, 2713, 2981, 3234, 4002
EcoT38I GRGCYC 2 cut(s) 1366, 3109
ErhI CCWWGG 7 cut(s) 828, 2073, 2692, 2713, 2981, 3234, 4002
FalI AAGNNNNNCTT 8 cut(s) 2202, 2234, 2850, 2882, 3036, 3068, 4074, 4106
FaqI GGGAC 6 cut(s) 79, 656, 1883, 2443, 3712, 4113
FauI CCCGC 1 cut(s) 744
FbaI TGATCA 4 cut(s) 688, 1570, 3295, 3661
FblI GTMKAC 2 cut(s) 837, 2166
FriOI GRGCYC 2 cut(s) 1366, 3109
FspBI CTAG 5 cut(s) 1061, 2928, 2996, 3167, 4309
GlaI GCGC 5 cut(s) 1956, 2022, 2146, 2772, 3623
GsaI CCCAGC 1 cut(s) 2021
GsuI CTGGAG 1 cut(s) 3654
HaeII RGCGCY 1 cut(s) 2024
HaeIII GGCC 7 cut(s) 5, 744, 1952, 2225, 2469, 2932, 3751
HapII CCGG 5 cut(s) 36, 1516, 2793, 3757, 4076
HgaI GACGC 4 cut(s) 76, 107, 3008, 3104
HhaI GCGC 5 cut(s) 1957, 2023, 2147, 2773, 3624
Hin1I GRCGYC 3 cut(s) 87, 99, 3096
Hin6I GCGC 5 cut(s) 1955, 2021, 2145, 2771, 3622
HinP1I GCGC 5 cut(s) 1955, 2021, 2145, 2771, 3622
HincII GTYRAC 3 cut(s) 2608, 3730, 3943
HindII GTYRAC 3 cut(s) 2608, 3730, 3943
HpaII CCGG 5 cut(s) 36, 1516, 2793, 3757, 4076
Hpy99I CGWCG 7 cut(s) 11, 55, 92, 95, 101, 2201, 3098
Hsp92I GRCGYC 3 cut(s) 87, 99, 3096
HspAI GCGC 5 cut(s) 1955, 2021, 2145, 2771, 3622
Kpn2I TCCGGA 1 cut(s) 3756
KpnI GGTACC 2 cut(s) 1606, 2196
KroI GCCGGC 1 cut(s) 2792
KroNI GCCGGC 1 cut(s) 2794
Ksp22I TGATCA 4 cut(s) 688, 1570, 3295, 3661
LguI GCTCTTC 2 cut(s) 914, 4095
LmnI GCTCC 7 cut(s) 1182, 1855, 2019, 2278, 2431, 3673, 3776
LweI GCATC 8 cut(s) 140, 146, 392, 450, 510, 704, 1519, 3016
MaeI CTAG 5 cut(s) 1061, 2928, 2996, 3167, 4309
MaeIII GTNAC 9 cut(s) 599, 635, 805, 863, 1147, 1667, 1672, 2375, 3133
MflI RGATCY 3 cut(s) 45, 3128, 3206
MhlI GDGCHC 6 cut(s) 1179, 1188, 1366, 3109, 3166, 3553
MlsI TGGCCA 3 cut(s) 744, 2225, 3751
MluNI TGGCCA 3 cut(s) 744, 2225, 3751
MmeI TCCRAC 3 cut(s) 425, 589, 2876
Mox20I TGGCCA 3 cut(s) 744, 2225, 3751
MroI TCCGGA 1 cut(s) 3756
MroNI GCCGGC 1 cut(s) 2792
MscI TGGCCA 3 cut(s) 744, 2225, 3751
MseI TTAA 9 cut(s) 479, 1430, 1434, 1961, 2472, 2685, 2702, 3074, 3786
MslI CAYNNNNRTG 2 cut(s) 2567, 4166
Msp20I TGGCCA 3 cut(s) 744, 2225, 3751
MspA1I CMGCKG 3 cut(s) 2404, 2490, 3215
MspCI CTTAAG 2 cut(s) 1960, 2471
MspI CCGG 5 cut(s) 36, 1516, 2793, 3757, 4076
MspR9I CCNGG 8 cut(s) 70, 84, 172, 741, 1517, 2136, 2412, 3039
MssI GTTTAAAC 1 cut(s) 480
Mva1269I GAATGC 1 cut(s) 3503
MvaI CCWGG 7 cut(s) 70, 84, 172, 741, 2136, 2412, 3039
MvnI CGCG 4 cut(s) 1955, 2145, 2244, 3624
NaeI GCCGGC 1 cut(s) 2794
NciI CCSGG 1 cut(s) 1517
NcoI CCATGG 2 cut(s) 2073, 3234
NgoMIV GCCGGC 1 cut(s) 2792
NlaIV GGNNCC 5 cut(s) 67, 1604, 2194, 2495, 3306
NmuCI GTSAC 5 cut(s) 863, 1667, 1672, 2375, 3133
NspI RCATGY 5 cut(s) 620, 1118, 1425, 1927, 3252
OliI CACNNNNGTG 1 cut(s) 2567
PacI TTAATTAA 1 cut(s) 1434
PaeI GCATGC 1 cut(s) 620
PaeR7I CTCGAG 1 cut(s) 3864
PaqCI CACCTGC 1 cut(s) 1941
PciI ACATGT 1 cut(s) 3248
PciSI GCTCTTC 2 cut(s) 914, 4095
PcsI WCGNNNNNNNCGW 4 cut(s) 99, 1336, 2196, 3016
PctI GAATGC 1 cut(s) 3503
PdiI GCCGGC 1 cut(s) 2794
PfeI GAWTC 6 cut(s) 175, 1088, 2745, 3851, 4033, 4039
PflMI CCANNNNNTGG 2 cut(s) 706, 3651
PfoI TCCNGGA 1 cut(s) 2410
PinAI ACCGGT 1 cut(s) 35
PmaCI CACGTG 1 cut(s) 3926
PmeI GTTTAAAC 1 cut(s) 480
PmlI CACGTG 1 cut(s) 3926
Ppu21I YACGTR 3 cut(s) 503, 3261, 3926
PpuMI RGGWCCY 2 cut(s) 66, 2689
PscI ACATGT 1 cut(s) 3248
PshBI ATTAAT 1 cut(s) 1434
PsiI TTATAA 1 cut(s) 2208
Psp124BI GAGCTC 1 cut(s) 1366
Psp1406I AACGTT 2 cut(s) 927, 3077
Psp5II RGGWCCY 2 cut(s) 66, 2689
Psp6I CCWGG 7 cut(s) 68, 82, 170, 739, 2134, 2410, 3037
PspCI CACGTG 1 cut(s) 3926
PspEI GGTNACC 1 cut(s) 2375
PspFI CCCAGC 1 cut(s) 2017
PspGI CCWGG 7 cut(s) 68, 82, 170, 739, 2134, 2410, 3037
PspN4I GGNNCC 5 cut(s) 67, 1604, 2194, 2495, 3306
PspPI GGNCC 8 cut(s) 66, 869, 2334, 2689, 2950, 3305, 4179, 4268
PspPPI RGGWCCY 2 cut(s) 66, 2689
PspXI VCTCGAGB 1 cut(s) 3864
PstI CTGCAG 1 cut(s) 1982
PstNI CAGNNNCTG 2 cut(s) 469, 4055
PsuI RGATCY 3 cut(s) 45, 3128, 3206
PvuII CAGCTG 3 cut(s) 2404, 2490, 3215
RseI CAYNNNNRTG 2 cut(s) 2567, 4166
Rsr2I CGGWCCG 1 cut(s) 869
RsrII CGGWCCG 1 cut(s) 869
SacI GAGCTC 1 cut(s) 1366
SapI GCTCTTC 2 cut(s) 914, 4095
SaqAI TTAA 9 cut(s) 479, 1430, 1434, 1961, 2472, 2685, 2702, 3074, 3786
Sau96I GGNCC 8 cut(s) 66, 869, 2334, 2689, 2950, 3305, 4179, 4268
ScaI AGTACT 1 cut(s) 3272
ScrFI CCNGG 8 cut(s) 70, 84, 172, 741, 1517, 2136, 2412, 3039
SduI GDGCHC 6 cut(s) 1179, 1188, 1366, 3109, 3166, 3553
SfaNI GCATC 8 cut(s) 140, 146, 392, 450, 510, 704, 1519, 3016
SfcI CTRYAG 1 cut(s) 1978
Sfr274I CTCGAG 1 cut(s) 3864
SgrAI CRCCGGYG 1 cut(s) 35
SinI GGWCC 8 cut(s) 66, 869, 2334, 2689, 2950, 3305, 4179, 4268
SlaI CTCGAG 1 cut(s) 3864
SmiMI CAYNNNNRTG 2 cut(s) 2567, 4166
SmlI CTYRAG 7 cut(s) 914, 1960, 2471, 2654, 2739, 2839, 3864
SmoI CTYRAG 7 cut(s) 914, 1960, 2471, 2654, 2739, 2839, 3864
SpeI ACTAGT 1 cut(s) 3166
SphI GCATGC 1 cut(s) 620
SsiI CCGC 7 cut(s) 111, 737, 794, 941, 1784, 1953, 3221
SspMI CTAG 5 cut(s) 1061, 2928, 2996, 3167, 4309
SstI GAGCTC 1 cut(s) 1366
StyD4I CCNGG 8 cut(s) 68, 82, 170, 739, 1515, 2134, 2410, 3037
StyI CCWWGG 7 cut(s) 828, 2073, 2692, 2713, 2981, 3234, 4002
TaqII GACCGA 4 cut(s) 833, 1832, 1898, 2938
TatI WGTACW 2 cut(s) 2178, 3270
TauI GCSGC 3 cut(s) 113, 797, 1955
TfiI GAWTC 6 cut(s) 175, 1088, 2745, 3851, 4033, 4039
Tru1I TTAA 9 cut(s) 479, 1430, 1434, 1961, 2472, 2685, 2702, 3074, 3786
Tru9I TTAA 9 cut(s) 479, 1430, 1434, 1961, 2472, 2685, 2702, 3074, 3786
TscAI CASTG 7 cut(s) 214, 652, 868, 2515, 3009, 3326, 3752
TseFI GTSAC 5 cut(s) 863, 1667, 1672, 2375, 3133
Tsp45I GTSAC 5 cut(s) 863, 1667, 1672, 2375, 3133
TspGWI ACGGA 7 cut(s) 1287, 1345, 1856, 2288, 2870, 3497, 4260
TspRI CASTG 7 cut(s) 214, 652, 868, 2515, 3009, 3326, 3752
Van91I CCANNNNNTGG 2 cut(s) 706, 3651
Vha464I CTTAAG 2 cut(s) 1960, 2471
VneI GTGCAC 1 cut(s) 3162
VpaK11BI GGWCC 8 cut(s) 66, 869, 2334, 2689, 2950, 3305, 4179, 4268
VspI ATTAAT 1 cut(s) 1434
XagI CCTNNNNNAGG 3 cut(s) 1971, 2384, 3042
XapI RAATTY 5 cut(s) 1237, 1654, 2501, 2578, 4289
XbaI TCTAGA 1 cut(s) 2995
XceI RCATGY 5 cut(s) 620, 1118, 1425, 1927, 3252
XcmI CCANNNNNNNNNTGG 2 cut(s) 752, 1395
XhoI CTCGAG 1 cut(s) 3864
XmiI GTMKAC 2 cut(s) 837, 2166
XspI CTAG 5 cut(s) 1061, 2928, 2996, 3167, 4309
ZrmI AGTACT 1 cut(s) 3272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.