Rw4G006650
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000071
Physical Location & Seq
Forward (+)
13787734 .. 13792302
4569 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006650.1

Sequence Viewer

Length: 2937 bp
ATGTCAAAATCAGAACTTTCTAATCCTTATTACACCCATCATTCAGATCATCCAGGGCTAGTCCTAGTCTCTAAACCTCTGAATGGAGACAATTATGCAGGGTGGAAGAGGGCTATGACTTTAGCTCTGAATTCCAAGAACAAGCTGGGCTTTGTGAATGGTACAATAACGGCCCCTTCAGAAGAGACCGATCCAGAAGGCTATGGTGCTTGGTCACGCTGCAATGATATGGTCCACTCATGGATCATTAACACAGTAGATCCAGAAATCAGTGACAGCGTCATCTACTATCCTACCGCACACGAAGTATGGGAAGATCTTCATGAACGGTTCTCTCAAGGTAACGCCCCACGAATCTTTGAAATTCAGCGAGAGATCGCCTGTCTCCGACAAGAACAGCTGTCAGTCTCAACCTATTATACAAAATTGAAAAGCCTATGGGATGAACTAGCTACCTATTCTGAGATATCTCGTGGACCACAGGCAGAACAACAAAGACTCATGCAATTTCTAATGGGGCTGAATGATACTTACAGTTCAGTACGCGGACAAATTCTTTTGATGGTTCCTTTGCCAACAGTCCGTAAAGCATATGCAGCGGTCGCTCAAGATGAGAAGCAACGTTCTCTTTGTGCTTCTCATCCCACTGCAGAATCCTCTAGCGCCGCGATGGCTGTGCGCAATCCAGGAAGATCCAATTACAATTCAGGAAGAGGGGGACGTTTTGAGAGGAATGATCGCCAAAACCAACGTCCAGATCGTAGTTTTGGGTCCCAGGAAGGACGCCGTGTTGACACTGAACGGCGTCAAGGATCCGGCAGGGGGAGACCGCATTGTACCTACTGTGGAGATCAGGGCCATTGGGTACAGACTTGTTATCAATTAATTGGGTATCCCCCAGGTCATCCCAAGGCAAAGCAGGGCACCGGTTTTTCGAAGCAATCAAGAGTGACAATGCCTTCAGCAAATCAGGTTAGTGAAGGAGGTGAAGATGGTAATGGGCTGACGGTGACTCTCACTGAAGCTCAGTTTAAACAGTTTATGGCTGCCCTTAGTAATCCGACTTTGAAGCCCGATTCAAATGCCAGCTCAGGTTCCAAAGCTAATGTTGTGACAAAACCAGGTTTGTCTAAAGTTGTTTCCCGCAGTTGGATCATTGACAGCGGTGCAACTGATCATATTACTTCTTCTACGCAATTATTGCATAAAAATAATAAATGCCCATTACCACCGGTTTTATTGCCTAGTGGAGATAAAGTTGACATTGTTGCAAAAGGATCAATACCACTAAGTACCATGTTTTATCTGCATGATGTGCTTTCTGTGCCTAAATTTAAAGATCTGGCTACGAGGAGGACGATTGGTTTGGGTGAACAACGCAACGGATTATATTATTTGATGGCATTAGCGACGGAGAATTCCAAAAACAATCCTTCCTTTTCCACCGCACGCCCAGCTTGTCATCTTACCGCTCTTCAAGCTAACAGAACTTGGACCCTGACTCCTCTTCCGGCCGGTAAGGTCCCTATTGGTTGTCGTTGGGTTTATAAGGTGAAACACAAGGCTGATGGTTCTGTGGAGCGGTATAAAGCCCGCTTGGTTGCTAAGGGTTTCACTCAGTTAGAAGGGGTCGATTATCAGGAAACTTTTTCTCCTACTGCCAAAATCATTACTGTTCGATGTTTGTTGGCTTTGGCTGCAGCTCGCGGTTGGACCTTGCATCAATTGGATGTGAATAATGCATTCCTCCATGGCGACTTGTCAGAGGAAATTTACATGTCTCCTCCGCCTGGTCTTCGGCGACAGGGGGAGGAGAATCTGGTATGCAGATTACATAAATCCTTATATGGTTTAAAGCAAGCTTCACGGCAGTGGTTTGAGAAGTTTTCTGATGCTGTACGGTCAGCTGGGTACGTCCAATCTAAAGCTGATTATTCATTATTCACTAGAAAGCATGGGAATTCATTTACTGCACTCTTGATCTATGTTGATGATATATTGATTACCGGAAATGATCTGGAGATTATTGCTGCACTTAAAGCTTTCTTACATAGTCAATTTCGTCTCAAAGATCTTGGAGACTTAAAGTACTTTCTTGGTATTGAAGTTTCGACTTCCAAACGGGGAATTTTTATATGTCAACGTAAGTATGCTCTAGAGATTATTGAAGATGCAGGGTTACTTGGAGCTGCACCTGTGGATACTCCCATGGAAAAGGGTTTAAAATTGTCTGATCATAGTGATTTACTCAAAGACCCTGGAAAGTACAGGAGGTTAATTGGCAGGCTTATATATCTCACTGTTTCTCGCCCTGACATCACTTATGCAGTCCATGTCCTTAGCAGATTCATGAATCAACCAAGAAAAATGCATTGGGAGGCAGCTTTGAGAGTAGTACGCTATCTAAAAGGCGCTCCTGGACAAGGTCTGTTTTTCTCTTCCACCAATGATCTGAAATTGAGGGCTTATTGTGACTCTGATTGGGCGGGATGCCCTGTCACTAGAAGGTCTACCACCGGATACTTTGTTTTTCTTGGACCTTCCTTAATTTCTTGGAGGTCAAAACGTCAAAAGACAGTATCTTTGTCTTCAGCAGAGGCGGAATATCGAGCTATGACAGGAACGTGTTGTGAGCTGACTTGGTTGCGTTATTTGCTTAGAGATCTAAATCTATCTCTCTCTGAACCGGCTCTGCTGTTTTGTGATAACAAGGCTGCACTGCATATTGCTGCCAACCCTGTATTCCATGAGCGAACTAGGCATATTGAGATGGATTGCCACTACATTAGAGACAAAATTCTAGATGGTACTGTGGCCACGAGGTATGTGAATTCAGCTTATCAACTTGCTGATGTTCTTACTAAACCTTTGGGGAAAGATGTCTTTGTCCCTATGATTCGCAAGTTGGGAGTGCAAGATATCCACTCTCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_3 Retrotrans_gag

Protein Analysis

978

Amino Acids

109.28

Weight (kDa)

9.28

Isoelectric Point (pI)

44.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 13 - 59 1.4e-22 gag-polypeptide of LTR copia-type
Retrotran_gag_2 PF14223 39 - 208 2.8e-07 gag-polypeptide of LTR copia-type
Retrotrans_gag PF03732 76 - 175 4.9e-06 Retrotransposon gag protein
Pol_BBD PF22936 384 - 446 2.8e-07 Pol polyprotein, beta-barrel domain
RVT_2 PF07727 495 - 739 2e-76 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1548
Acc16I TGCGCA 1 cut(s) 680
AccB1I GGYRCC 1 cut(s) 923
AccBSI CCGCTC 2 cut(s) 1472, 1582
AccI GTMKAC 1 cut(s) 2510
AccII CGCG 3 cut(s) 546, 668, 1707
AclI AACGTT 1 cut(s) 622
AclWI GGATC 8 cut(s) 185, 251, 254, 687, 807, 820, 1160, 1285
AcoI YGGCCR 2 cut(s) 1512, 2814
AcuI CTGAAG 4 cut(s) 162, 945, 1041, 2574
AcyI GRCGYC 2 cut(s) 784, 805
AfiI CCNNNNNNNGG 5 cut(s) 83, 822, 1149, 1790, 2423
AflIII ACRYGT 2 cut(s) 1776, 2624
AgeI ACCGGT 2 cut(s) 926, 1231
AgsI TTSAA 7 cut(s) 362, 430, 1069, 1080, 1478, 2105, 2168
AjnI CCWGG 8 cut(s) 52, 685, 774, 898, 1120, 1789, 2257, 2416
AjuI GAANNNNNNNTTGG 4 cut(s) 2356, 2388, 2726, 2758
AleI CACNNNNGTG 1 cut(s) 1870
AlwI GGATC 8 cut(s) 185, 251, 254, 687, 807, 820, 1160, 1285
AoxI GGCC 4 cut(s) 171, 856, 1512, 2814
ArsI GACNNNNNNTTYG 6 cut(s) 736, 768, 1348, 1380, 2566, 2598
AseI ATTAAT 1 cut(s) 884
AsiGI ACCGGT 2 cut(s) 926, 1231
Asp700I GAANNNNTTC 1 cut(s) 318
AspLEI GCGC 3 cut(s) 665, 681, 2414
AspS9I GGNCC 9 cut(s) 172, 232, 476, 771, 856, 1494, 1522, 1713, 2537
AsuHPI GGTGA 4 cut(s) 998, 1021, 1382, 1564
AsuII TTCGAA 1 cut(s) 935
AvaII GGWCC 7 cut(s) 232, 476, 771, 1494, 1522, 1713, 2537
BaeGI GKGCMC 1 cut(s) 926
BalI TGGCCA 1 cut(s) 2816
BamHI GGATCC 1 cut(s) 812
BanI GGYRCC 1 cut(s) 923
BauI CACGAG 2 cut(s) 471, 2818
BbsI GAAGAC 2 cut(s) 1787, 2580
BccI CCATC 8 cut(s) 45, 556, 664, 986, 1393, 1562, 2764, 2798
BceAI ACGGC 4 cut(s) 186, 771, 818, 1883
BcgI CGANNNNNNTGC 4 cut(s) 658, 692, 1064, 1098
BciT130I CCWGG 8 cut(s) 54, 687, 776, 900, 1122, 1791, 2259, 2418
BciVI GTATCC 3 cut(s) 903, 2194, 2513
BclI TGATCA 2 cut(s) 1174, 2233
BfmI CTRYAG 2 cut(s) 648, 1698
BfoI RGCGCY 2 cut(s) 666, 2415
BfuI GTATCC 3 cut(s) 903, 2194, 2513
BglI GCCNNNNNGGC 1 cut(s) 671
BglII AGATCT 4 cut(s) 316, 1339, 2071, 2662
BmcAI AGTACT 1 cut(s) 2090
Bme1390I CCNGG 8 cut(s) 54, 687, 776, 900, 1122, 1791, 2259, 2418
Bme18I GGWCC 7 cut(s) 232, 476, 771, 1494, 1522, 1713, 2537
BmgT120I GGNCC 9 cut(s) 172, 232, 476, 771, 856, 1494, 1522, 1713, 2537
BmiI GGNNCC 9 cut(s) 174, 567, 772, 773, 814, 925, 1096, 1496, 1524
BmrFI CCNGG 8 cut(s) 54, 687, 776, 900, 1122, 1791, 2259, 2418
BmsI GCATC 4 cut(s) 1729, 1882, 2161, 2480
BpiI GAAGAC 2 cut(s) 1787, 2580
BpmI CTGGAG 1 cut(s) 2039
Bpu10I CCTNAGC 3 cut(s) 1090, 1605, 2339
Bpu14I TTCGAA 1 cut(s) 935
BpuEI CTTGAG 2 cut(s) 321, 591
BsaBI GATNNNNATC 1 cut(s) 2667
BsaHI GRCGYC 2 cut(s) 784, 805
BsaI GGTCTC 2 cut(s) 179, 820
BsaJI CCNNGG 7 cut(s) 53, 774, 898, 909, 1750, 2208, 2257
BsaWI WCCGGW 4 cut(s) 926, 1231, 2006, 2516
BsaXI ACNNNNNCTCC 6 cut(s) 1242, 1272, 1486, 1516, 1636, 1666
Bsc4I CCNNNNNNNGG 5 cut(s) 83, 822, 1149, 1790, 2423
Bse118I RCCGGY 4 cut(s) 926, 1231, 1514, 2686
Bse3DI GCAATG 1 cut(s) 229
Bse8I GATNNNNATC 1 cut(s) 2667
BseBI CCWGG 8 cut(s) 54, 687, 776, 900, 1122, 1791, 2259, 2418
BseDI CCNNGG 7 cut(s) 53, 774, 898, 909, 1750, 2208, 2257
BseGI GGATG 6 cut(s) 49, 448, 640, 904, 1735, 2495
BseJI GATNNNNATC 1 cut(s) 2667
BseLI CCNNNNNNNGG 5 cut(s) 83, 822, 1149, 1790, 2423
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 4 cut(s) 453, 1040, 1104, 1631
BseRI GAGGAG 4 cut(s) 1366, 1494, 1773, 1826
BseSI GKGCMC 1 cut(s) 926
BseX3I CGGCCG 1 cut(s) 1512
BseYI CCCAGC 3 cut(s) 145, 1453, 1907
BsgI GTGCAG 4 cut(s) 1956, 2016, 2175, 2700
Bsh1236I CGCG 3 cut(s) 546, 668, 1707
Bsh1285I CGRYCG 2 cut(s) 603, 1515
BshFI GGCC 4 cut(s) 173, 858, 1514, 2816
BshNI GGYRCC 1 cut(s) 923
BshTI ACCGGT 2 cut(s) 926, 1231
BsiEI CGRYCG 2 cut(s) 603, 1515
BsiSI CCGG 8 cut(s) 816, 927, 1232, 1511, 1515, 2007, 2517, 2687
BslFI GGGAC 4 cut(s) 732, 757, 1508, 2873
BslI CCNNNNNNNGG 5 cut(s) 83, 822, 1149, 1790, 2423
BsmBI CGTCTC 1 cut(s) 2069
BsmFI GGGAC 4 cut(s) 732, 757, 1508, 2873
BsmI GAATGC 1 cut(s) 1742
BsnI GGCC 4 cut(s) 173, 858, 1514, 2816
Bso31I GGTCTC 2 cut(s) 179, 820
Bsp119I TTCGAA 1 cut(s) 935
Bsp1286I GDGCHC 1 cut(s) 926
Bsp19I CCATGG 2 cut(s) 1750, 2208
BspANI GGCC 4 cut(s) 173, 858, 1514, 2816
BspCNI CTCAG 4 cut(s) 454, 1039, 1103, 1630
BspFNI CGCG 3 cut(s) 546, 668, 1707
BspHI TCATGA 2 cut(s) 322, 2349
BspLI GGNNCC 9 cut(s) 174, 567, 772, 773, 814, 925, 1096, 1496, 1524
BspMAI CTGCAG 2 cut(s) 652, 1702
BspPI GGATC 8 cut(s) 185, 251, 254, 687, 807, 820, 1160, 1285
BspQI GCTCTTC 1 cut(s) 1479
BspT104I TTCGAA 1 cut(s) 935
BspT107I GGYRCC 1 cut(s) 923
BspTNI GGTCTC 2 cut(s) 179, 820
BsrBI CCGCTC 2 cut(s) 1472, 1582
BsrDI GCAATG 1 cut(s) 229
BsrFI RCCGGY 4 cut(s) 926, 1231, 1514, 2686
BssAI RCCGGY 4 cut(s) 926, 1231, 1514, 2686
BssECI CCNNGG 7 cut(s) 53, 774, 898, 909, 1750, 2208, 2257
BssNI GRCGYC 2 cut(s) 784, 805
BssSI CACGAG 2 cut(s) 471, 2818
BssT1I CCWWGG 3 cut(s) 909, 1750, 2208
Bst2BI CACGAG 2 cut(s) 471, 2818
Bst2UI CCWGG 8 cut(s) 54, 687, 776, 900, 1122, 1791, 2259, 2418
Bst6I CTCTTC 6 cut(s) 101, 177, 706, 1479, 1512, 2443
BstACI GRCGYC 2 cut(s) 784, 805
BstAPI GCANNNNNTGC 2 cut(s) 1201, 1315
BstBI TTCGAA 1 cut(s) 935
BstC8I GCNNGC 6 cut(s) 1087, 1450, 1594, 1705, 1860, 2285
BstDEI CTNAG 9 cut(s) 462, 1026, 1052, 1090, 1289, 1605, 1617, 2339, 2657
BstDSI CCRYGG 2 cut(s) 1750, 2208
BstENI CCTNNNNNAGG 1 cut(s) 2421
BstF5I GGATG 6 cut(s) 49, 448, 640, 904, 1735, 2495
BstFNI CGCG 3 cut(s) 546, 668, 1707
BstH2I RGCGCY 2 cut(s) 666, 2415
BstHHI GCGC 3 cut(s) 665, 681, 2414
BstMCI CGRYCG 2 cut(s) 603, 1515
BstNI CCWGG 8 cut(s) 54, 687, 776, 900, 1122, 1791, 2259, 2418
BstNSI RCATGY 1 cut(s) 1780
BstSCI CCNGG 8 cut(s) 52, 685, 774, 898, 1120, 1789, 2257, 2416
BstSFI CTRYAG 2 cut(s) 648, 1698
BstSLI GKGCMC 1 cut(s) 926
BstUI CGCG 3 cut(s) 546, 668, 1707
BstV2I GAAGAC 2 cut(s) 1787, 2580
BstX2I RGATCY 7 cut(s) 259, 316, 692, 812, 1339, 2071, 2662
BstYI RGATCY 7 cut(s) 259, 316, 692, 812, 1339, 2071, 2662
BstZI CGGCCG 1 cut(s) 1512
BsuI GTATCC 3 cut(s) 903, 2194, 2513
BsuRI GGCC 4 cut(s) 173, 858, 1514, 2816
BtgI CCRYGG 2 cut(s) 1750, 2208
BtgZI GCGATG 1 cut(s) 683
BtsCI GGATG 6 cut(s) 49, 448, 640, 904, 1735, 2495
BtsI GCAGTG 3 cut(s) 645, 1877, 2717
BtsIMutI CAGTG 7 cut(s) 277, 645, 795, 1017, 1877, 2298, 2717
Cac8I GCNNGC 6 cut(s) 1087, 1450, 1594, 1705, 1860, 2285
CciI TCATGA 2 cut(s) 322, 2349
CfoI GCGC 3 cut(s) 665, 681, 2414
Cfr10I RCCGGY 4 cut(s) 926, 1231, 1514, 2686
Cfr13I GGNCC 9 cut(s) 172, 232, 476, 771, 856, 1494, 1522, 1713, 2537
CseI GACGC 3 cut(s) 268, 792, 794
CsiI ACCWGGT 1 cut(s) 1120
CspAI ACCGGT 2 cut(s) 926, 1231
DdeI CTNAG 9 cut(s) 462, 1026, 1052, 1090, 1289, 1605, 1617, 2339, 2657
DraI TTTAAA 4 cut(s) 1033, 1336, 1854, 2223
EaeI YGGCCR 2 cut(s) 1512, 2814
EagI CGGCCG 1 cut(s) 1512
Eam1104I CTCTTC 6 cut(s) 101, 177, 706, 1479, 1512, 2443
EarI CTCTTC 6 cut(s) 101, 177, 706, 1479, 1512, 2443
EciI GGCGGA 2 cut(s) 1776, 2615
EclXI CGGCCG 1 cut(s) 1512
Eco130I CCWWGG 3 cut(s) 909, 1750, 2208
Eco31I GGTCTC 2 cut(s) 179, 820
Eco32I GATATC 2 cut(s) 468, 2920
Eco47I GGWCC 7 cut(s) 232, 476, 771, 1494, 1522, 1713, 2537
Eco52I CGGCCG 1 cut(s) 1512
Eco57I CTGAAG 4 cut(s) 162, 945, 1041, 2574
EcoNI CCTNNNNNAGG 1 cut(s) 2421
EcoO109I RGGNCCY 2 cut(s) 771, 1522
EcoRI GAATTC 4 cut(s) 130, 1417, 1960, 2830
EcoRII CCWGG 8 cut(s) 52, 685, 774, 898, 1120, 1789, 2257, 2416
EcoRV GATATC 2 cut(s) 468, 2920
EcoT14I CCWWGG 3 cut(s) 909, 1750, 2208
EcoT22I ATGCAT 2 cut(s) 1744, 2373
ErhI CCWWGG 3 cut(s) 909, 1750, 2208
Esp3I CGTCTC 1 cut(s) 2069
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 4 cut(s) 732, 757, 1508, 2873
FauI CCCGC 3 cut(s) 1151, 1601, 2479
FauNDI CATATG 1 cut(s) 592
FbaI TGATCA 2 cut(s) 1174, 2233
FblI GTMKAC 1 cut(s) 2510
FokI GGATG 6 cut(s) 36, 455, 627, 891, 1742, 2502
FspI TGCGCA 1 cut(s) 680
GlaI GCGC 3 cut(s) 664, 680, 2413
GsaI CCCAGC 3 cut(s) 149, 1457, 1911
GsuI CTGGAG 1 cut(s) 2039
HaeII RGCGCY 2 cut(s) 666, 2415
HaeIII GGCC 4 cut(s) 173, 858, 1514, 2816
HapII CCGG 8 cut(s) 816, 927, 1232, 1511, 1515, 2007, 2517, 2687
HgaI GACGC 3 cut(s) 268, 792, 794
HhaI GCGC 3 cut(s) 665, 681, 2414
Hin1I GRCGYC 2 cut(s) 784, 805
Hin6I GCGC 3 cut(s) 663, 679, 2412
HinP1I GCGC 3 cut(s) 663, 679, 2412
HincII GTYRAC 3 cut(s) 793, 1261, 2141
HindII GTYRAC 3 cut(s) 793, 1261, 2141
HindIII AAGCTT 2 cut(s) 1860, 2040
HpaII CCGG 8 cut(s) 816, 927, 1232, 1511, 1515, 2007, 2517, 2687
HphI GGTGA 4 cut(s) 998, 1021, 1382, 1564
Hpy166II GTNNAC 7 cut(s) 235, 476, 793, 1261, 1373, 2141, 2511
Hpy8I GTNNAC 7 cut(s) 235, 476, 793, 1261, 1373, 2141, 2511
Hpy99I CGWCG 1 cut(s) 1414
HpyAV CCTTC 9 cut(s) 186, 191, 773, 969, 974, 1443, 1619, 2499, 2550
HpyCH4IV ACGT 7 cut(s) 622, 721, 751, 1914, 2143, 2566, 2624
HpyF3I CTNAG 9 cut(s) 462, 1026, 1052, 1090, 1289, 1605, 1617, 2339, 2657
HpySE526I ACGT 7 cut(s) 622, 721, 751, 1914, 2143, 2566, 2624
Hsp92I GRCGYC 2 cut(s) 784, 805
HspAI GCGC 3 cut(s) 663, 679, 2412
KflI GGGWCCC 1 cut(s) 771
Ksp22I TGATCA 2 cut(s) 1174, 2233
LguI GCTCTTC 1 cut(s) 1479
LmnI GCTCC 3 cut(s) 1579, 2186, 2419
LweI GCATC 4 cut(s) 1729, 1882, 2161, 2480
MabI ACCWGGT 1 cut(s) 1120
MaeII ACGT 7 cut(s) 622, 721, 751, 1914, 2143, 2566, 2624
MaeIII GTNAC 9 cut(s) 213, 272, 341, 949, 1009, 1111, 2178, 2471, 2497
MbiI CCGCTC 2 cut(s) 1472, 1582
MfeI CAATTG 1 cut(s) 1724
MflI RGATCY 7 cut(s) 259, 316, 692, 812, 1339, 2071, 2662
MhlI GDGCHC 1 cut(s) 926
MlsI TGGCCA 1 cut(s) 2816
MluNI TGGCCA 1 cut(s) 2816
MlyI GAGTC 4 cut(s) 492, 1006, 1495, 2468
MmeI TCCRAC 4 cut(s) 412, 1085, 1130, 1691
Mox20I TGGCCA 1 cut(s) 2816
Mph1103I ATGCAT 2 cut(s) 1744, 2373
MroXI GAANNNNTTC 1 cut(s) 318
MscI TGGCCA 1 cut(s) 2816
MslI CAYNNNNRTG 1 cut(s) 1870
Msp20I TGGCCA 1 cut(s) 2816
MspA1I CMGCKG 4 cut(s) 400, 599, 1164, 1907
MspI CCGG 8 cut(s) 816, 927, 1232, 1511, 1515, 2007, 2517, 2687
MspR9I CCNGG 8 cut(s) 54, 687, 776, 900, 1122, 1791, 2259, 2418
MssI GTTTAAAC 1 cut(s) 1033
MunI CAATTG 1 cut(s) 1724
Mva1269I GAATGC 1 cut(s) 1742
MvaI CCWGG 8 cut(s) 54, 687, 776, 900, 1122, 1791, 2259, 2418
MvnI CGCG 3 cut(s) 546, 668, 1707
NcoI CCATGG 2 cut(s) 1750, 2208
NdeI CATATG 1 cut(s) 592
NlaIV GGNNCC 9 cut(s) 174, 567, 772, 773, 814, 925, 1096, 1496, 1524
NmuCI GTSAC 7 cut(s) 213, 272, 949, 1009, 1111, 2471, 2497
NsbI TGCGCA 1 cut(s) 680
NsiI ATGCAT 2 cut(s) 1744, 2373
NspI RCATGY 1 cut(s) 1780
NspV TTCGAA 1 cut(s) 935
OliI CACNNNNGTG 1 cut(s) 1870
PagI TCATGA 2 cut(s) 322, 2349
PciI ACATGT 1 cut(s) 1776
PciSI GCTCTTC 1 cut(s) 1479
PcsI WCGNNNNNNNCGW 2 cut(s) 757, 1355
PctI GAATGC 1 cut(s) 1742
PdmI GAANNNNTTC 1 cut(s) 318
PfeI GAWTC 7 cut(s) 354, 653, 1076, 1816, 2346, 2353, 2896
PflFI GACNNNGTC 2 cut(s) 278, 2424
PfoI TCCNGGA 2 cut(s) 685, 2416
PinAI ACCGGT 2 cut(s) 926, 1231
PleI GAGTC 4 cut(s) 492, 1006, 1495, 2468
PmeI GTTTAAAC 1 cut(s) 1033
PpsI GAGTC 4 cut(s) 492, 1006, 1495, 2468
PpuMI RGGWCCY 2 cut(s) 771, 1522
PscI ACATGT 1 cut(s) 1776
PshBI ATTAAT 1 cut(s) 884
PsiI TTATAA 1 cut(s) 1548
Psp1406I AACGTT 1 cut(s) 622
Psp5II RGGWCCY 2 cut(s) 771, 1522
Psp6I CCWGG 8 cut(s) 52, 685, 774, 898, 1120, 1789, 2257, 2416
PspFI CCCAGC 3 cut(s) 145, 1453, 1907
PspGI CCWGG 8 cut(s) 52, 685, 774, 898, 1120, 1789, 2257, 2416
PspN4I GGNNCC 9 cut(s) 174, 567, 772, 773, 814, 925, 1096, 1496, 1524
PspPI GGNCC 9 cut(s) 172, 232, 476, 771, 856, 1494, 1522, 1713, 2537
PspPPI RGGWCCY 2 cut(s) 771, 1522
PstI CTGCAG 2 cut(s) 652, 1702
PsuI RGATCY 7 cut(s) 259, 316, 692, 812, 1339, 2071, 2662
PsyI GACNNNGTC 2 cut(s) 278, 2424
PvuII CAGCTG 2 cut(s) 400, 1907
RseI CAYNNNNRTG 1 cut(s) 1870
SapI GCTCTTC 1 cut(s) 1479
Sau96I GGNCC 9 cut(s) 172, 232, 476, 771, 856, 1494, 1522, 1713, 2537
ScaI AGTACT 1 cut(s) 2090
SchI GAGTC 4 cut(s) 492, 1006, 1495, 2468
ScrFI CCNGG 8 cut(s) 54, 687, 776, 900, 1122, 1791, 2259, 2418
SduI GDGCHC 1 cut(s) 926
SexAI ACCWGGT 1 cut(s) 1120
SfaNI GCATC 4 cut(s) 1729, 1882, 2161, 2480
SfcI CTRYAG 2 cut(s) 648, 1698
SfuI TTCGAA 1 cut(s) 935
SinI GGWCC 7 cut(s) 232, 476, 771, 1494, 1522, 1713, 2537
SmiMI CAYNNNNRTG 1 cut(s) 1870
SmlI CTYRAG 2 cut(s) 336, 606
SmoI CTYRAG 2 cut(s) 336, 606
StyD4I CCNGG 8 cut(s) 52, 685, 774, 898, 1120, 1789, 2257, 2416
StyI CCWWGG 3 cut(s) 909, 1750, 2208
TaiI ACGT 7 cut(s) 625, 724, 754, 1917, 2146, 2569, 2627
TaqI TCGA 5 cut(s) 935, 1632, 1678, 2111, 2608
TaqII GACCGA 1 cut(s) 203
TatI WGTACW 2 cut(s) 2088, 2265
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 7 cut(s) 354, 653, 1076, 1816, 2346, 2353, 2896
TscAI CASTG 7 cut(s) 277, 652, 802, 1024, 1877, 2305, 2724
TseFI GTSAC 7 cut(s) 213, 272, 949, 1009, 1111, 2471, 2497
Tsp45I GTSAC 7 cut(s) 213, 272, 949, 1009, 1111, 2471, 2497
TspDTI ATGAA 7 cut(s) 311, 339, 459, 1926, 1953, 2338, 2366
TspGWI ACGGA 3 cut(s) 572, 1398, 1427
TspRI CASTG 7 cut(s) 277, 652, 802, 1024, 1877, 2305, 2724
Tth111I GACNNNGTC 2 cut(s) 278, 2424
VpaK11BI GGWCC 7 cut(s) 232, 476, 771, 1494, 1522, 1713, 2537
VspI ATTAAT 1 cut(s) 884
XagI CCTNNNNNAGG 1 cut(s) 2421
XbaI TCTAGA 2 cut(s) 2155, 2800
XceI RCATGY 1 cut(s) 1780
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 2510
XmnI GAANNNNTTC 1 cut(s) 318
ZrmI AGTACT 1 cut(s) 2090
Zsp2I ATGCAT 2 cut(s) 1744, 2373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.