RchiOBHm_Chr2g0101201

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000071
Physical Location & Seq
Reverse (-)
12875141 .. 12879405
4265 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47582

Sequence Viewer

Length: 3528 bp
ATGGTGAAAGCTCCTTCTTCTATGCGTCCCTCTCCTCAAGGTTCTTCCCCCGTGTTCTCCTCAAGTCTGACTTGTAACTACTGCAAGAATCCAGGCCATATCAAAGCAAATTGTTACAGGTTGGTTGGTTTTCTAGCAGGCTACTTTGATAGGCCCCGACCTCAGGACAACAAACCCAAGGGAAAGGCTGCAGTGACAAAACCAGATCACACCAACTTTACTGGTAAGGATACCGCATCCGTAAGTTGCGGTGGTAATGGTAAGATTGGAGTTGCTTTAAAAATTTCTGGCTTTACTGGTGGCAATACTTGGATAATTGATTCTGGAGCATCTGACCATATGACCTATGATCGTAGTTTCTTTCTTTTTCTCAATCCACCCTCTATTTCCTCTGTTGTCAATGCCAATGGTGATTCTTTTCCTGTTTTAGGAATAGGGTCTGTTCGTCTCACCCCTTCTTTGACACTACATGATGTTCTTTATGTCCCTGATCTCTCTCATCATCTTATATCTGTGCCTCAGCTTAATACTCAATCTCGGTGTTCTGTAACATTTTTTCCGTTGTATGTGATTTTTCAGGACCTCCTCACCAGGGAAATAATCGGCAGGGGTTATCTGAGGGGTCGACTATTTCATCTGGATTGCATGTTCGCCGGAATGAAGCCGGAGAAGGCAGTCCAAGCAGCTTTGATTTCGACTGGGGTTACTAATCAAGTTGAAGAATTATGGCTATGGCATCGGAGATTGGGACATCCTTCCTTTAGTGTCATGAAGAAATCTATGCCATCCTTGTTTCTGGGCATTGATGAGTCTAAGTTGCATTGTGAGTCATGTGTCTTAGCTAAAAGTCATCGTACTAGTTATCCTTTGAGTACTACTAGGAGTTCTTCTCCTTTTGAGCTTATTCATTCTGATGTCTGGGGACCTTCCCGTGAGTCAACCTTGTCTGGAAAACATTGGTTTTTTCACCAATTCTTTCATTCTCATGGGATTATTCATCAAACTACTTGCCCTCAAACCCCTGAACAAAATGGGGTGTCAGAAAGAAAGAATCGTCATGTTCTTGACATAGCCCGGTCTCATCTATTTAGTGCTCATATGCCTAAGTATCTTTGGGGAGATGCAATTCTCACTGCTTGTCATCTCATTAATAGGCTACCTTCCTCTGTTCTCAATGGTCAAACCCCCTATGCTGCCCTTTCAAATCATGTGTCTGTCCCTTCGTTTTCTAATCTCCCTGCTCGTGTCTTTGGGTGTGTTGTGTTCGTCCATGTTCCTAAGAATCAAAGGTCCAAACTCGATGCCAGGGCATTAAAATGTGTTTTTGTGGGCTATGGTGTTAATCAGAAGGGCTACAAGTGTTTCCATCCCCCTACACAGAAAGTTTTTGTTACCATGGATGTCACCTTTTATGAGGATGCATGTTACTTTTCTCCCATCAATCCTTCACTTCAGGGGGAGAAACACACTTTTTTGGAAGAGAAGTCTTGTGGGATTGAAATTCAGCCCATTGAAGCCAACGTCACTCCTGCCGAAACGGAAAAGTCAATTGCCGAACAGAGCATTGCGAGTTCCGAAATAGAAACGGCGATTGCTGAATCGTCTACCCTTCGCCATGCAACTGCCGAACAGAGCATTGCGAGTTCCGAAACAGAAACGGCGATTGCTGAATCGTCTACCCTTCGCCATGCAACTGCCGAACAGAGCATTGCGAGTTCCGAAATAGAAAAGACAATTGCCGAATCATCTACCCTCCGAGAAGGCCAAGCTCCTCCACCCCTCTTAGATACAGATAACCCTTGTCAACCAATCCTTGAGGAGCGTCCCAATCTTGAGGTTAGTGGTGTTTCTTCAAACTCTGATATTGGTGATAGTGTGCATGTTTTGCCTCCTAGGTCCACTAGTGGTAAACCACCTCGTCAATATGAACCAAGTTTAGATGTTAAGTCTAAGTATGCCATAGCTAACTTTGTATCCACCCACCGGTTATCCAAATCCCATGCTTCTTTTGTGAATCAAATATCCTCTGTATGTGTTCCCAGTAAAGTGCAGGATGCTCTCAAGGATCCCAAATGGTCTCAAGCAATGAATGAAGAGATGGAGGCATTGGAGAAGAATAATACTTGGAAGTTGGTGCCACCTCCACAAGGAAAGAGAGTTGTTGGATGTAGATGGGTGTACACTATCAAACATAATGCAGATGGTTCAGTGAACAGGTATAAAGCAAGACTTGTTGCAAAGGGATATACTCAAACATATGGCGTGGATTATGAGGAGACCTTTGCTCCAGTAGCAAAGATTAATACTGTTCGAGTGTTGATGTCTCTAGCAGCTAATCTAGATTGGCCCTTGCAACAGTTTGATGTGAAGAATGCCTTTTTACATGGAGAGCTATCTGAAGAAGAGGTTGAAAGATTAAAAGATCTTCTTGCATCAGAGTTTGAGATGAAAGATCTTGGTTCTCTTAAATATTTTTTGGGGATTGAGGTCGCCAGGGGGAGTTCGGGAATTTTCTTGTGCCAGAGAAAATATGTGCTTGATTTGTTGACAGAGACAGGAATGCTAGGGTGTAGACCTGCTGATACTCCTATTGAGCAGAATCATAAACTGGCTGAGTACCCTAATCAAACTCTTACAAAGAAGGCTCGCTATCAGAGGTTAGTTGGTAGATTAATCTATCTCTCACATACTCGGCCAGACATTGCATATGCAGTCAGTGTTGTGAGTCAATTCATGCATAATCCAAGTGAAACTCATATGGAAGCTGTGATCCGTATTCTGAGGTATTTAAAGTCTGCACCTGGAAAAGGCTTGATGTTTTCAAAGCATAGTCATCTGGATATTAGTGGTTATACAGATGCAGACTGGGCAGGTTGTGTTACGGATAGAAAATCCACTTCTGGTTACTTCACTTTTGTAGGTGGTAATCTTGTTACTTGGAGAAGTAAGAAACAAAAGGTTGTGGCCAGGTCAAGTGCTGAAGCAGAGTATAGAGGTATGGCTCATGGAGTTTGTGAATTACTTTGGTTGAGAAATTTACTTCGTGATTTGGGATTTAAACCCAAACGAGCCATGAATTTGTATTGTGATAACAAGGCAGCGGTGGATATTGCTCACAACCCAGTTCAACATGACAGAACTAAGCATGTTGAGGTTGACAGACATTTCATTAAAGAGAAGCTCGATGCTAAGCTTATCTCTTTTCCTTTTGTCCACTCAGAAGAACAGTTAGCTGATGTTCTCACAAAAGCAGTATCGATATCTCTTAATTCTCAAACTCAAAATCGATTTCTGAACAATCATAAAACTGCTCCAACACAATGCACAAGTAAAAAAATACGGAAATGGTCAAAAGGGTCCATCTGTGCTAGCTGCTTCTTAGGCCTCAAAGAAGCAGTAAAGTCGAAATCTTTAACTCGGAGAAGGACCTCCACGGCTGAGCTGGGCCTCGAGGTCTCGGCCCCAAGCCCAAAGCTGACCTTCGGAAGTGACGGCCAGGGATTGGTAAGGCCCGGCGGTGACTGGTGA

Protein Analysis

1175

Amino Acids

130.35

Weight (kDa)

8.98

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 104 - 177 1e-18 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 232 - 283 8.8e-15 GAG-pre-integrase domain
SH3_retrovirus PF25597 419 - 476 1.2e-23 Retroviral polymerase SH3-like domain
RVT_2 PF07727 706 - 801 3e-29 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2 PF07727 802 - 865 1.3e-10 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 3449
Acc36I ACCTGC 2 cut(s) 2584, 2861
AccB1I GGYRCC 1 cut(s) 2134
AccB7I CCANNNNNTGG 1 cut(s) 3502
AccI GTMKAC 4 cut(s) 625, 1604, 1676, 2572
AciI CCGC 4 cut(s) 234, 249, 3101, 3516
AclWI GGATC 3 cut(s) 2060, 2073, 2764
AcoI YGGCCR 3 cut(s) 2693, 2964, 3493
AcsI RAATTY 5 cut(s) 282, 1500, 2508, 3034, 3076
AcuI CTGAAG 3 cut(s) 1436, 2418, 3000
AfaI GTAC 4 cut(s) 856, 874, 2180, 2618
AfiI CCNNNNNNNGG 7 cut(s) 163, 428, 592, 1983, 2147, 2807, 3502
AgeI ACCGGT 1 cut(s) 1983
AgsI TTSAA 8 cut(s) 719, 1203, 1499, 1514, 1854, 2411, 2823, 3128
AhlI ACTAGT 2 cut(s) 857, 1901
AjnI CCWGG 7 cut(s) 91, 590, 1304, 2492, 2800, 2966, 3495
AloI GAACNNNNNNTCC 2 cut(s) 632, 664
Alw21I GWGCWC 1 cut(s) 1096
Alw26I GTCTC 7 cut(s) 452, 1083, 2082, 2270, 2328, 2546, 3460
AlwI GGATC 3 cut(s) 2060, 2073, 2764
Ama87I CYCGRG 1 cut(s) 3449
ApeKI GCWGC 6 cut(s) 188, 683, 1193, 2330, 3098, 3372
ApoI RAATTY 5 cut(s) 282, 1500, 2508, 3034, 3076
ArsI GACNNNNNNTTYG 2 cut(s) 1196, 1228
AseI ATTAAT 3 cut(s) 1149, 2301, 2672
AsiGI ACCGGT 1 cut(s) 1983
AspA2I CCTAGG 1 cut(s) 1892
AsuC2I CCSGG 2 cut(s) 1075, 3513
AsuHPI GGTGA 7 cut(s) 16, 422, 442, 580, 959, 1396, 1880
AsuNHI GCTAGC 1 cut(s) 3368
AvaI CYCGRG 1 cut(s) 3449
AvaII GGWCC 6 cut(s) 580, 923, 1290, 1896, 3357, 3426
AvrII CCTAGG 1 cut(s) 1892
AxyI CCTNAGG 1 cut(s) 162
BalI TGGCCA 1 cut(s) 2966
BamHI GGATCC 1 cut(s) 2065
BanI GGYRCC 1 cut(s) 2134
BauI CACGAG 1 cut(s) 1242
Bbv12I GWGCWC 1 cut(s) 1096
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 6 cut(s) 175, 695, 1180, 2342, 3110, 3359
BccI CCATC 7 cut(s) 793, 1374, 1445, 2092, 2166, 2195, 3368
BceAI ACGGC 4 cut(s) 1602, 1674, 3450, 3508
BcgI CGANNNNNNTGC 2 cut(s) 3384, 3418
BciT130I CCWGG 7 cut(s) 93, 592, 1306, 2494, 2802, 2968, 3497
BciVI GTATCC 2 cut(s) 223, 1984
BcnI CCSGG 2 cut(s) 1075, 3513
BcoDI GTCTC 7 cut(s) 452, 1083, 2082, 2270, 2328, 2546, 3460
BcuI ACTAGT 2 cut(s) 857, 1901
BfaI CTAG 9 cut(s) 134, 858, 879, 1893, 1902, 2327, 2339, 2564, 3369
BfmI CTRYAG 1 cut(s) 189
BfuAI ACCTGC 2 cut(s) 2584, 2861
BfuI GTATCC 2 cut(s) 223, 1984
BglII AGATCT 2 cut(s) 2422, 2452
BisI GCNGC 6 cut(s) 189, 684, 1194, 2331, 3099, 3373
BlnI CCTAGG 1 cut(s) 1892
BlpI GCTNAGC 2 cut(s) 3189, 3438
BlsI GCNGC 6 cut(s) 190, 685, 1195, 2332, 3100, 3374
BmcAI AGTACT 1 cut(s) 874
Bme1390I CCNGG 9 cut(s) 93, 592, 1075, 1306, 2494, 2802, 2968, 3497, 3513
Bme18I GGWCC 6 cut(s) 580, 923, 1290, 1896, 3357, 3426
BmeT110I CYCGRG 1 cut(s) 3449
BmiI GGNNCC 6 cut(s) 155, 924, 2067, 2136, 3358, 3462
BmrFI CCNGG 9 cut(s) 93, 592, 1075, 1306, 2494, 2802, 2968, 3497, 3513
BmrI ACTGGG 4 cut(s) 708, 2034, 2875, 3116
BmtI GCTAGC 1 cut(s) 3372
BmuI ACTGGG 4 cut(s) 708, 2034, 2875, 3116
BplI GAGNNNNNCTC 2 cut(s) 874, 906
BpmI CTGGAG 2 cut(s) 345, 2271
Bpu10I CCTNAGC 1 cut(s) 519
Bpu1102I GCTNAGC 2 cut(s) 3189, 3438
BpuEI CTTGAG 6 cut(s) 21, 46, 1835, 1853, 2045, 2064
BpuMI CCSGG 2 cut(s) 1075, 3513
Bsa29I ATCGAT 2 cut(s) 3257, 3286
BsaI GGTCTC 4 cut(s) 1083, 2082, 2270, 3460
BsaJI CCNNGG 8 cut(s) 177, 591, 1305, 1395, 1892, 2493, 3432, 3496
BsaWI WCCGGW 1 cut(s) 1983
BsaXI ACNNNNNCTCC 2 cut(s) 2269, 2299
Bsc4I CCNNNNNNNGG 7 cut(s) 163, 428, 592, 1983, 2147, 2807, 3502
Bse118I RCCGGY 1 cut(s) 1983
Bse1I ACTGG 9 cut(s) 226, 301, 703, 2040, 2288, 2613, 2870, 3122, 3527
Bse21I CCTNAGG 1 cut(s) 162
Bse3DI GCAATG 5 cut(s) 1563, 1635, 1707, 2091, 2700
BseBI CCWGG 7 cut(s) 93, 592, 1306, 2494, 2802, 2968, 3497
BseCI ATCGAT 2 cut(s) 3257, 3286
BseDI CCNNGG 8 cut(s) 177, 591, 1305, 1395, 1892, 2493, 3432, 3496
BseGI GGATG 8 cut(s) 236, 751, 785, 1366, 1405, 1423, 2059, 2171
BseLI CCNNNNNNNGG 7 cut(s) 163, 428, 592, 1983, 2147, 2807, 3502
BseMI GCAATG 5 cut(s) 1563, 1635, 1707, 2091, 2700
BseMII CTCAG 7 cut(s) 176, 533, 608, 2604, 2771, 3231, 3429
BseNI ACTGG 9 cut(s) 226, 301, 703, 2040, 2288, 2613, 2870, 3122, 3527
BseRI GAGGAG 6 cut(s) 24, 49, 575, 1761, 1832, 2288
BseXI GCAGC 6 cut(s) 175, 695, 1180, 2342, 3110, 3359
BseYI CCCAGC 1 cut(s) 3442
BsgI GTGCAG 2 cut(s) 2069, 2781
BshNI GGYRCC 1 cut(s) 2134
BshTI ACCGGT 1 cut(s) 1983
BshVI ATCGAT 2 cut(s) 3257, 3286
BsiHKAI GWGCWC 1 cut(s) 1096
BsiHKCI CYCGRG 1 cut(s) 3449
BsiSI CCGG 5 cut(s) 654, 665, 1075, 1984, 3513
BslFI GGGAC 6 cut(s) 12, 470, 762, 936, 1202, 1809
BslI CCNNNNNNNGG 7 cut(s) 163, 428, 592, 1983, 2147, 2807, 3502
BsmAI GTCTC 7 cut(s) 452, 1083, 2082, 2270, 2328, 2546, 3460
BsmBI CGTCTC 1 cut(s) 452
BsmFI GGGAC 6 cut(s) 12, 470, 762, 936, 1202, 1809
BsmI GAATGC 2 cut(s) 2377, 2565
Bso31I GGTCTC 4 cut(s) 1083, 2082, 2270, 3460
BsoBI CYCGRG 1 cut(s) 3449
Bsp1286I GDGCHC 1 cut(s) 1096
Bsp1407I TGTACA 1 cut(s) 2178
Bsp143I GATC 7 cut(s) 205, 349, 490, 2065, 2422, 2452, 2769
Bsp1720I GCTNAGC 2 cut(s) 3189, 3438
Bsp19I CCATGG 1 cut(s) 1395
BspACI CCGC 4 cut(s) 234, 249, 3101, 3516
BspCNI CTCAG 7 cut(s) 175, 532, 609, 2605, 2772, 3230, 3430
BspDI ATCGAT 2 cut(s) 3257, 3286
BspHI TCATGA 1 cut(s) 768
BspLI GGNNCC 6 cut(s) 155, 924, 2067, 2136, 3358, 3462
BspMAI CTGCAG 1 cut(s) 193
BspMI ACCTGC 2 cut(s) 2584, 2861
BspOI GCTAGC 1 cut(s) 3372
BspPI GGATC 3 cut(s) 2060, 2073, 2764
BspT107I GGYRCC 1 cut(s) 2134
BspTNI GGTCTC 4 cut(s) 1083, 2082, 2270, 3460
BsrDI GCAATG 5 cut(s) 1563, 1635, 1707, 2091, 2700
BsrFI RCCGGY 1 cut(s) 1983
BsrGI TGTACA 1 cut(s) 2178
BsrI ACTGG 9 cut(s) 226, 301, 703, 2040, 2288, 2613, 2870, 3122, 3527
BssAI RCCGGY 1 cut(s) 1983
BssECI CCNNGG 8 cut(s) 177, 591, 1305, 1395, 1892, 2493, 3432, 3496
BssMI GATC 7 cut(s) 205, 349, 490, 2065, 2422, 2452, 2769
BssSI CACGAG 1 cut(s) 1242
BssT1I CCWWGG 3 cut(s) 177, 1395, 1892
Bst2BI CACGAG 1 cut(s) 1242
Bst2UI CCWGG 7 cut(s) 93, 592, 1306, 2494, 2802, 2968, 3497
Bst4CI ACNGT 3 cut(s) 2308, 2358, 3228
Bst6I CTCTTC 3 cut(s) 1473, 2088, 2397
BstAPI GCANNNNNTGC 1 cut(s) 1885
BstAUI TGTACA 1 cut(s) 2178
BstC8I GCNNGC 3 cut(s) 139, 2647, 3370
BstDSI CCRYGG 2 cut(s) 1395, 3432
BstENI CCTNNNNNAGG 3 cut(s) 426, 2145, 2805
BstF5I GGATG 8 cut(s) 236, 751, 785, 1366, 1405, 1423, 2059, 2171
BstKTI GATC 7 cut(s) 208, 352, 493, 2068, 2425, 2455, 2772
BstMAI GTCTC 7 cut(s) 452, 1083, 2082, 2270, 2328, 2546, 3460
BstMBI GATC 7 cut(s) 205, 349, 490, 2065, 2422, 2452, 2769
BstMWI GCNNNNNNNGC 5 cut(s) 680, 1885, 2291, 2867, 3381
BstNI CCWGG 7 cut(s) 93, 592, 1306, 2494, 2802, 2968, 3497
BstNSI RCATGY 4 cut(s) 649, 1425, 1883, 3149
BstSCI CCNGG 9 cut(s) 91, 590, 1073, 1304, 2492, 2800, 2966, 3495, 3511
BstSFI CTRYAG 1 cut(s) 189
BstV1I GCAGC 6 cut(s) 175, 695, 1180, 2342, 3110, 3359
BstX2I RGATCY 3 cut(s) 2065, 2422, 2452
BstYI RGATCY 3 cut(s) 2065, 2422, 2452
Bsu15I ATCGAT 2 cut(s) 3257, 3286
Bsu36I CCTNAGG 1 cut(s) 162
BsuI GTATCC 2 cut(s) 223, 1984
BsuTUI ATCGAT 2 cut(s) 3257, 3286
BtgI CCRYGG 2 cut(s) 1395, 3432
BtsCI GGATG 8 cut(s) 236, 751, 785, 1366, 1405, 1423, 2059, 2171
BtsI GCAGTG 2 cut(s) 198, 1131
BtsIMutI CAGTG 4 cut(s) 198, 1131, 2214, 2722
BveI ACCTGC 2 cut(s) 2584, 2861
Cac8I GCNNGC 3 cut(s) 139, 2647, 3370
CciI TCATGA 1 cut(s) 768
Cfr10I RCCGGY 1 cut(s) 1983
ClaI ATCGAT 2 cut(s) 3257, 3286
CseI GACGC 2 cut(s) 14, 1811
Csp6I GTAC 4 cut(s) 855, 873, 2179, 2617
CspAI ACCGGT 1 cut(s) 1983
CviQI GTAC 4 cut(s) 855, 873, 2179, 2617
DpnI GATC 7 cut(s) 207, 351, 492, 2067, 2424, 2454, 2771
DpnII GATC 7 cut(s) 205, 349, 490, 2065, 2422, 2452, 2769
DraI TTTAAA 3 cut(s) 279, 2790, 3058
EaeI YGGCCR 3 cut(s) 2693, 2964, 3493
Eam1104I CTCTTC 3 cut(s) 1473, 2088, 2397
EarI CTCTTC 3 cut(s) 1473, 2088, 2397
Eco130I CCWWGG 3 cut(s) 177, 1395, 1892
Eco147I AGGCCT 1 cut(s) 3384
Eco31I GGTCTC 4 cut(s) 1083, 2082, 2270, 3460
Eco32I GATATC 1 cut(s) 3261
Eco47I GGWCC 6 cut(s) 580, 923, 1290, 1896, 3357, 3426
Eco57I CTGAAG 3 cut(s) 1436, 2418, 3000
Eco81I CCTNAGG 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 3449
EcoNI CCTNNNNNAGG 3 cut(s) 426, 2145, 2805
EcoO109I RGGNCCY 4 cut(s) 153, 580, 923, 3426
EcoRII CCWGG 7 cut(s) 91, 590, 1304, 2492, 2800, 2966, 3495
EcoRV GATATC 1 cut(s) 3261
EcoT14I CCWWGG 3 cut(s) 177, 1395, 1892
EcoT22I ATGCAT 2 cut(s) 1423, 2739
ErhI CCWWGG 3 cut(s) 177, 1395, 1892
Esp3I CGTCTC 1 cut(s) 452
FaqI GGGAC 6 cut(s) 12, 470, 762, 936, 1202, 1809
FauNDI CATATG 5 cut(s) 339, 1098, 2257, 2707, 2757
FblI GTMKAC 4 cut(s) 625, 1604, 1676, 2572
Fnu4HI GCNGC 6 cut(s) 189, 684, 1194, 2331, 3099, 3373
FokI GGATG 8 cut(s) 223, 738, 772, 1353, 1412, 1430, 2066, 2178
Fsp4HI GCNGC 6 cut(s) 189, 684, 1194, 2331, 3099, 3373
FspBI CTAG 9 cut(s) 134, 858, 879, 1893, 1902, 2327, 2339, 2564, 3369
GluI GCNGC 6 cut(s) 189, 684, 1194, 2331, 3099, 3373
GsaI CCCAGC 1 cut(s) 3446
GsuI CTGGAG 2 cut(s) 345, 2271
HapII CCGG 5 cut(s) 654, 665, 1075, 1984, 3513
HgaI GACGC 2 cut(s) 14, 1811
HincII GTYRAC 5 cut(s) 626, 939, 1805, 2547, 3157
HindII GTYRAC 5 cut(s) 626, 939, 1805, 2547, 3157
HindIII AAGCTT 1 cut(s) 3191
HpaII CCGG 5 cut(s) 654, 665, 1075, 1984, 3513
HphI GGTGA 7 cut(s) 16, 422, 442, 580, 959, 1396, 1880
HpyCH4III ACNGT 3 cut(s) 2308, 2358, 3228
HpyCH4IV ACGT 1 cut(s) 1521
HpyF10VI GCNNNNNNNGC 5 cut(s) 680, 1885, 2291, 2867, 3381
HpySE526I ACGT 1 cut(s) 1521
Kzo9I GATC 7 cut(s) 205, 349, 490, 2065, 2422, 2452, 2769
LmnI GCTCC 6 cut(s) 16, 326, 1774, 1819, 2290, 3316
Lsp1109I GCAGC 6 cut(s) 175, 695, 1180, 2342, 3110, 3359
MaeI CTAG 9 cut(s) 134, 858, 879, 1893, 1902, 2327, 2339, 2564, 3369
MaeII ACGT 1 cut(s) 1521
MalI GATC 7 cut(s) 207, 351, 492, 2067, 2424, 2454, 2771
MboI GATC 7 cut(s) 205, 349, 490, 2065, 2422, 2452, 2769
MfeI CAATTG 2 cut(s) 1548, 1734
MflI RGATCY 3 cut(s) 2065, 2422, 2452
MhlI GDGCHC 1 cut(s) 1096
MlsI TGGCCA 1 cut(s) 2966
MluNI TGGCCA 1 cut(s) 2966
MlyI GAGTC 4 cut(s) 818, 836, 944, 2734
MmeI TCCRAC 2 cut(s) 2143, 3338
Mox20I TGGCCA 1 cut(s) 2966
Mph1103I ATGCAT 2 cut(s) 1423, 2739
MscI TGGCCA 1 cut(s) 2966
MslI CAYNNNNRTG 3 cut(s) 912, 984, 1315
Msp20I TGGCCA 1 cut(s) 2966
MspA1I CMGCKG 1 cut(s) 3101
MspI CCGG 5 cut(s) 654, 665, 1075, 1984, 3513
MspR9I CCNGG 9 cut(s) 93, 592, 1075, 1306, 2494, 2802, 2968, 3497, 3513
MunI CAATTG 2 cut(s) 1548, 1734
Mva1269I GAATGC 2 cut(s) 2377, 2565
MvaI CCWGG 7 cut(s) 93, 592, 1306, 2494, 2802, 2968, 3497
MwoI GCNNNNNNNGC 5 cut(s) 680, 1885, 2291, 2867, 3381
NciI CCSGG 2 cut(s) 1075, 3513
NcoI CCATGG 1 cut(s) 1395
NdeI CATATG 5 cut(s) 339, 1098, 2257, 2707, 2757
NdeII GATC 7 cut(s) 205, 349, 490, 2065, 2422, 2452, 2769
NheI GCTAGC 1 cut(s) 3368
NlaIV GGNNCC 6 cut(s) 155, 924, 2067, 2136, 3358, 3462
NmeAIII GCCGAG 2 cut(s) 2671, 3437
NmuCI GTSAC 5 cut(s) 193, 1402, 1522, 3488, 3518
NsiI ATGCAT 2 cut(s) 1423, 2739
NspI RCATGY 4 cut(s) 649, 1425, 1883, 3149
PaeR7I CTCGAG 1 cut(s) 3449
PagI TCATGA 1 cut(s) 768
PceI AGGCCT 1 cut(s) 3384
PctI GAATGC 2 cut(s) 2377, 2565
PflMI CCANNNNNTGG 1 cut(s) 3502
PinAI ACCGGT 1 cut(s) 1983
PkrI GCNGC 6 cut(s) 190, 685, 1195, 2332, 3100, 3374
PleI GAGTC 4 cut(s) 817, 835, 943, 2733
PpsI GAGTC 4 cut(s) 817, 835, 943, 2733
PpuMI RGGWCCY 3 cut(s) 580, 923, 3426
PshBI ATTAAT 3 cut(s) 1149, 2301, 2672
Psp5II RGGWCCY 3 cut(s) 580, 923, 3426
Psp6I CCWGG 7 cut(s) 91, 590, 1304, 2492, 2800, 2966, 3495
PspFI CCCAGC 1 cut(s) 3442
PspGI CCWGG 7 cut(s) 91, 590, 1304, 2492, 2800, 2966, 3495
PspN4I GGNNCC 6 cut(s) 155, 924, 2067, 2136, 3358, 3462
PspPPI RGGWCCY 3 cut(s) 580, 923, 3426
PspXI VCTCGAGB 1 cut(s) 3449
PstI CTGCAG 1 cut(s) 193
PsuI RGATCY 3 cut(s) 2065, 2422, 2452
RsaI GTAC 4 cut(s) 856, 874, 2180, 2618
RsaNI GTAC 4 cut(s) 855, 873, 2179, 2617
RseI CAYNNNNRTG 3 cut(s) 912, 984, 1315
SalI GTCGAC 1 cut(s) 624
SatI GCNGC 6 cut(s) 189, 684, 1194, 2331, 3099, 3373
Sau3AI GATC 7 cut(s) 205, 349, 490, 2065, 2422, 2452, 2769
ScaI AGTACT 1 cut(s) 874
SchI GAGTC 4 cut(s) 818, 836, 944, 2734
ScrFI CCNGG 9 cut(s) 93, 592, 1075, 1306, 2494, 2802, 2968, 3497, 3513
SduI GDGCHC 1 cut(s) 1096
SfcI CTRYAG 1 cut(s) 189
Sfr274I CTCGAG 1 cut(s) 3449
SinI GGWCC 6 cut(s) 580, 923, 1290, 1896, 3357, 3426
SlaI CTCGAG 1 cut(s) 3449
SmiMI CAYNNNNRTG 3 cut(s) 912, 984, 1315
SmlI CTYRAG 7 cut(s) 36, 61, 1814, 1832, 2060, 2079, 3449
SmoI CTYRAG 7 cut(s) 36, 61, 1814, 1832, 2060, 2079, 3449
SpeI ACTAGT 2 cut(s) 857, 1901
SseBI AGGCCT 1 cut(s) 3384
SsiI CCGC 4 cut(s) 234, 249, 3101, 3516
SspI AATATT 1 cut(s) 2471
SspMI CTAG 9 cut(s) 134, 858, 879, 1893, 1902, 2327, 2339, 2564, 3369
StuI AGGCCT 1 cut(s) 3384
StyD4I CCNGG 9 cut(s) 91, 590, 1073, 1304, 2492, 2800, 2966, 3495, 3511
StyI CCWWGG 3 cut(s) 177, 1395, 1892
TaaI ACNGT 3 cut(s) 2308, 2358, 3228
TaiI ACGT 1 cut(s) 1524
TaqI TCGA 9 cut(s) 625, 695, 1299, 2311, 3183, 3257, 3286, 3404, 3450
TatI WGTACW 2 cut(s) 872, 2178
TscAI CASTG 4 cut(s) 198, 1138, 2214, 2722
TseFI GTSAC 5 cut(s) 193, 1402, 1522, 3488, 3518
TseI GCWGC 6 cut(s) 188, 683, 1193, 2330, 3098, 3372
Tsp45I GTSAC 5 cut(s) 193, 1402, 1522, 3488, 3518
TspGWI ACGGA 6 cut(s) 229, 549, 1553, 2762, 2897, 3355
TspRI CASTG 4 cut(s) 198, 1138, 2214, 2722
Van91I CCANNNNNTGG 1 cut(s) 3502
VpaK11BI GGWCC 6 cut(s) 580, 923, 1290, 1896, 3357, 3426
VspI ATTAAT 3 cut(s) 1149, 2301, 2672
XagI CCTNNNNNAGG 3 cut(s) 426, 2145, 2805
XapI RAATTY 5 cut(s) 282, 1500, 2508, 3034, 3076
XbaI TCTAGA 1 cut(s) 2338
XceI RCATGY 4 cut(s) 649, 1425, 1883, 3149
XcmI CCANNNNNNNNNTGG 1 cut(s) 3439
XhoI CTCGAG 1 cut(s) 3449
XmaJI CCTAGG 1 cut(s) 1892
XmiI GTMKAC 4 cut(s) 625, 1604, 1676, 2572
XspI CTAG 9 cut(s) 134, 858, 879, 1893, 1902, 2327, 2339, 2564, 3369
ZrmI AGTACT 1 cut(s) 874
Zsp2I ATGCAT 2 cut(s) 1423, 2739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.