RchiOBHm_Chr6g0298961

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000071
Physical Location & Seq
Forward (+)
60192912 .. 60196579
3668 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26842

Sequence Viewer

Length: 3351 bp
ATGAGGGGTAAGGTATCTGTGAATAGTTCTTGGATAATTGATACAGGTGCATCTGACCATATGACTAATGATCCTAGCCTCTTGAAACAGTTGAAACCCTCCTCGCAAAATATTGTTTCTACTGCTGATGGTACTCCAACTCCGGTAACCGGAGAAGGCTCTGTTGTCCTATTTGACACGTTAACCCTTGACTCTGTATTGGTTGTCCCTTCATTAGCATATAATCTCCTATCTGTTAGCCAGATTATCCTAGCACTAGCATGTATTGTGACTTTCTATCCGTCTTTCTGTGTGTTTCAGGACATTCTGACTCGGCGGATTCTTGGTTATGATGTTAGAAGGGGGAAGTTATACTACCTGGACCTGACAGAGACTGGAGAGAAACATAAGCATCTTTTGGGCCAGGGACAGGCTAATCAGATCAACGGGGTAGAGAATGCGAAGGAAGCTGTATGGTTATGGCATCGCCGTTTAGGCCATCTATCTTTTAGTTATCTTAAGAAGCTGCAACCTCATTTGTTTTCAGTTGTCAGTGATTCAGATTTCCATTGTGACATTTGTGAATTGGCCAAGAGCCACCATATTTCATATTCACCAAGTCTTCATAAAAGTCCTGTTCTTTTTATGAAAATTCACTCTGATGTCTGGGGTCCTGCGAAAATTCCTTCTCTTTCTGGAGCTCGGTATTTTGTAACGTTTATTGATGATTGCACTCGAATGACATGGGTGTCATTACTGAAGAATAAGAGTGATGTATTCGGGATGTTTACTGAATTTCACAAAATGGTGGCAACTCAGTATCAACAATCCATCAGAGTGTTTCAGTCTGACAATGGTGGAGAGTTTGTGAATGGCCCTATGATTGAGTTTTGCCGGTCACATGGAATTCGTCATCAGACCTCTAATTCTTATACTCCTCAACAGAATGGTTTAGCAGAACGGAAGAACAGGCAGTTGATCATGAATGTACCTCGGTCCTATTGGGGAGAAGCAGTGAAATCAGCAGCATATCTTATCAACCGTACTCCTTCGCGGGTGATTGAGTTTCAGAATCCTCATCAAAAGCTTCATACACTTCTGACCATCCCTTCCATGCCTAATTTGGAGCCCCGAGTGTTTGGATGCACAGCTTATGTTCATATTCCCAAGCCTCAACGCAGCAAGCTTGATCCCCGTGCCCGTAAGTGTATCTTTGTTGGTTATGCTGAATTTAAAAAGGGTTATCGCTGTTATGATCCTCTTACTGGCACTATACATGTCTCTCTTGACATTGTTTTTCGTGAATCCGAGCCTTATTATTCAAAGGGAGTTTCGGCGTCTTCCCTTCAGGAGGAGAAAGATTGTGAAGGAAATCATCGAGATTCATTATTTGAAGAATTTGAAGAATTAGAAGCCTTGGAATCAAGATTTGGAGTTGGAAATTCTTCAACAAAGGCGGACAACGAAACTGAAATCGAAATAGAAGCTAGGGCAGTAGAAAACGCCCAAGAAGATGTATTAACTGGCGGTGGACCAACAAGCAATAAAGATGCTGCCGAAACAGAAAAAGAAGAGGCCGTGGGGCCCTCCACCTGCGAAACAGAAAAAGCAGAGTGCGAAATAGAAAAAGAAGAGGCCGAGGGGCCCTCTACCTACGAAACAGAAAAAGAAGAGGCCGTGGGGCCCTCTGGATGCGAGATCATTAAGGATGCTGCCAACAGACCGGTTTTTAGCTCCAACGGTGGACCAGTGGAGGGACCAGTGGGGTCACCACCTGCAAAAATAGCAAAGAAGCTGCCAGCCGACACCATAATCTGTCAAATAGGACAGATGCAGCACTCCAGCATTCCCAAGGTGAAGACGCTTCAATGGATCCTCATTTATCAGAGTTCTTCCCCCTTTCTACATCCTCAACTGAAGAATCTGCTGCGAATGTTCCTCCTCAGTGTACAGGATGCTTTGGCGGATCCGAAGTGGACTCAAGCGATGAATGAAGAACTAGAGGCTTTGCAGAAAAACTTGACTTGGGATGTTGTAACTATGCCTGTTGGAAAGAAGACTGTTGGATGTCGGTGGGTATTTACAGTAAAACTGAAAGCTGATGGGAGCATTGACAGGTACAAAGCCAGATTGGTTGCCAAAGGATACACTCAGCGCTATGGAATTGATTATGAAGAGACATTTGCCCCTGTAGCAATGATCAATACTGTTCGAATCTTGATTTCACTTGCAGCAACTAAAGATTGGCCTCTACATCAGTTTGATGTGAAGAATGCTTTCCTCAATGGAAACTTGGAGGAAGAAGTGTATATGGACATGCCGCCAGGAGTCAAAAATATGCCTTGCGATACTGGCAAGGTTTGTAAGTTGAAGAAGTCTTTGTATGGTTTGAAACAGTCTCCTAGAGCTTGGTTTGGGAGATTCTCCAAGTCTATGAGGATGTTCGGATATAAACAAAGCAACTCAGATCATACCTTGTTCATTAAGCGTAGACAGGATAAGATTACCGCTCTGATAGTATATGTTGATGATATGATTGTCACAGGGGATGATCTAAAAGAGATAGAGGCCCTGCAGAAGTATCTTTCTAAGGAATTTGAGATGAAGGACCTTGGGCAATTGAAGTACTTTCTTGGAATTGAAGTCGCAAGGTCTAAGAAGGGGATTTCTTTATCCCAACGTAAGTATGTACTTGACTTGTTAGCTGAGACCGGAATGCTTGACTGCAACCCTATTGAGACACCGATCGAGATGAACCACAACCTTGCCATCTATCCGGATCAGGTTTCAACTGATAAAGGAAGGTACCAACGTCTAGTCGGGAGGCTTATTTATCTTTCTCACACTAGACCTGATATTGCTTATGCTATGAGTGTGGTTAGTCAATTTATGCATTGTCCCAGTGAAGAGCATATAGATGCAGTGTATCGTATTTTGAGATATCTTAAAATGGCACCTGGAAGAGGTTTATTGTTCGAAAAGAAGGGTGAATTGGAAGTTGTGGGGTACACAGATGCAGACTGGGCTGGTGATAAGACTGATAGACGGTCTACATCTGGGTATTTTACATTTGTTGGAGGAAACCTTGTGACTTGGCGCAGTAAGAAACAGAAGGTTAAACCTATGGACTTGCATTGTGATAGCACATCTGCTATTGAAATTGCTCATAATCCTGTTCAGCATGATCGGACTAAGCATGTGGAGGTAGATCGACACTTTATTAAGGAAAACCTGGATAGGAAAATCATCTGTTTCCCGTTCGTGTATACAGAAGATCAGTTAGCAGATGTTCTTACGAAAGGAGTGTCAAGGAAAGTGTTTGACAACTTAATTGGCAAGTTGGGCATGATCGATATCTATGCACCAACTTGA

Protein Analysis

1116

Amino Acids

125.93

Weight (kDa)

7.18

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 11 - 82 4e-15 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 138 - 192 1.4e-15 GAG-pre-integrase domain
rve PF00665 211 - 306 2.2e-11 Integrase core domain
SH3_retrovirus PF25597 375 - 433 1.5e-25 Retroviral polymerase SH3-like domain
RVT_2 PF07727 667 - 910 2e-76 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1580, 1762
AasI GACNNNNNNGTC 1 cut(s) 727
Acc36I ACCTGC 2 cut(s) 1580, 1762
Acc65I GGTACC 1 cut(s) 2784
AccB1I GGYRCC 2 cut(s) 2784, 2932
AccBSI CCGCTC 1 cut(s) 2489
AccI GTMKAC 3 cut(s) 2470, 3029, 3245
AccII CGCG 1 cut(s) 1033
AccIII TCCGGA 1 cut(s) 2755
AciI CCGC 7 cut(s) 316, 1033, 1436, 1506, 1943, 2300, 2487
AclI AACGTT 1 cut(s) 695
AclWI GGATC 8 cut(s) 65, 1163, 1229, 1846, 1859, 1940, 1953, 2766
AcoI YGGCCR 1 cut(s) 567
AcsI RAATTY 8 cut(s) 630, 660, 773, 885, 1208, 1376, 1420, 2573
AcuI CTGAAG 3 cut(s) 758, 1310, 1916
AcyI GRCGYC 1 cut(s) 1316
AfaI GTAC 9 cut(s) 133, 969, 1024, 1929, 2099, 2606, 2670, 2786, 2987
AfeI AGCGCT 1 cut(s) 2135
AfiI CCNNNNNNNGG 6 cut(s) 149, 409, 1244, 1330, 1733, 2942
AflII CTTAAG 1 cut(s) 497
AflIII ACRYGT 2 cut(s) 177, 1255
AgeI ACCGGT 1 cut(s) 1702
AjnI CCWGG 5 cut(s) 357, 402, 2302, 2935, 3210
AjuI GAANNNNNNNTTGG 4 cut(s) 1392, 1424, 2954, 2986
Alw21I GWGCWC 1 cut(s) 682
Alw26I GTCTC 6 cut(s) 365, 1264, 2150, 2382, 2681, 2711
AlwI GGATC 8 cut(s) 65, 1163, 1229, 1846, 1859, 1940, 1953, 2766
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 1110
Aor13HI TCCGGA 1 cut(s) 2755
Aor51HI AGCGCT 1 cut(s) 2135
ApaI GGGCCC 3 cut(s) 1566, 1626, 1665
ApeKI GCWGC 9 cut(s) 505, 1004, 1158, 1532, 1691, 1774, 1813, 1906, 2210
ApoI RAATTY 8 cut(s) 630, 660, 773, 885, 1208, 1376, 1420, 2573
ArsI GACNNNNNNTTYG 2 cut(s) 3269, 3301
AsiGI ACCGGT 1 cut(s) 1702
Asp700I GAANNNNTTC 2 cut(s) 1423, 2255
Asp718I GGTACC 1 cut(s) 2784
AspLEI GCGC 2 cut(s) 2136, 3078
AsuHPI GGTGA 6 cut(s) 585, 1048, 1740, 1846, 2978, 3020
AsuII TTCGAA 2 cut(s) 2191, 2955
AvaI CYCGRG 1 cut(s) 1110
AvaII GGWCC 7 cut(s) 361, 650, 975, 1511, 1724, 1736, 2587
BaeGI GKGCMC 4 cut(s) 1180, 1566, 1626, 1665
BaeI ACNNNNGTAYC 2 cut(s) 2776, 2809
BalI TGGCCA 1 cut(s) 569
BamHI GGATCC 2 cut(s) 1851, 1945
BanI GGYRCC 2 cut(s) 2784, 2932
BanII GRGCYC 5 cut(s) 682, 1110, 1566, 1626, 1665
BarI GAAGNNNNNNTAC 2 cut(s) 338, 370
BbsI GAAGAC 4 cut(s) 593, 1311, 1844, 2042
Bbv12I GWGCWC 1 cut(s) 682
BbvI GCAGC 9 cut(s) 492, 1016, 1170, 1519, 1678, 1761, 1825, 1893, 2222
BccI CCATC 6 cut(s) 122, 486, 818, 1091, 2075, 2756
BceAI ACGGC 3 cut(s) 453, 1541, 1640
BcgI CGANNNNNNTGC 1 cut(s) 3320
BciT130I CCWGG 5 cut(s) 359, 404, 2304, 2937, 3212
BciVI GTATCC 1 cut(s) 2117
BclI TGATCA 2 cut(s) 957, 2178
BcoDI GTCTC 6 cut(s) 365, 1264, 2150, 2382, 2681, 2711
BfaI CTAG 8 cut(s) 75, 251, 257, 1467, 1979, 2382, 2795, 2826
BfmI CTRYAG 2 cut(s) 2169, 2552
BfoI RGCGCY 1 cut(s) 2137
BfrI CTTAAG 1 cut(s) 497
BfuAI ACCTGC 2 cut(s) 1580, 1762
BfuI GTATCC 1 cut(s) 2117
BglI GCCNNNNNGGC 1 cut(s) 474
BmcAI AGTACT 1 cut(s) 2606
Bme1390I CCNGG 5 cut(s) 359, 404, 2304, 2937, 3212
Bme18I GGWCC 7 cut(s) 361, 650, 975, 1511, 1724, 1736, 2587
BmeT110I CYCGRG 1 cut(s) 1110
BmrFI CCNGG 5 cut(s) 359, 404, 2304, 2937, 3212
BmrI ACTGGG 2 cut(s) 2874, 3010
BmuI ACTGGG 2 cut(s) 2874, 3010
BpiI GAAGAC 4 cut(s) 593, 1311, 1844, 2042
BplI GAGNNNNNCTC 2 cut(s) 1610, 1642
BpmI CTGGAG 3 cut(s) 396, 696, 1804
Bpu14I TTCGAA 2 cut(s) 2191, 2955
BpuEI CTTGAG 1 cut(s) 1944
Bsa29I ATCGAT 1 cut(s) 3330
BsaBI GATNNNNATC 1 cut(s) 3332
BsaHI GRCGYC 1 cut(s) 1316
BsaI GGTCTC 1 cut(s) 2681
BsaJI CCNNGG 8 cut(s) 403, 971, 1395, 1557, 1617, 1656, 1830, 2590
BsaWI WCCGGW 5 cut(s) 142, 149, 1702, 2690, 2755
BsaXI ACNNNNNCTCC 6 cut(s) 124, 154, 831, 861, 978, 1008
Bsc4I CCNNNNNNNGG 6 cut(s) 149, 409, 1244, 1330, 1733, 2942
Bse118I RCCGGY 2 cut(s) 873, 1702
Bse1I ACTGG 8 cut(s) 379, 1249, 1507, 1727, 1739, 2335, 2880, 3005
Bse3DI GCAATG 1 cut(s) 2181
Bse8I GATNNNNATC 1 cut(s) 3332
BseAI TCCGGA 1 cut(s) 2755
BseBI CCWGG 5 cut(s) 359, 404, 2304, 2937, 3212
BseCI ATCGAT 1 cut(s) 3330
BseDI CCNNGG 8 cut(s) 403, 971, 1395, 1557, 1617, 1656, 1830, 2590
BseJI GATNNNNATC 1 cut(s) 3332
BseLI CCNNNNNNNGG 6 cut(s) 149, 409, 1244, 1330, 1733, 2942
BseMI GCAATG 1 cut(s) 2181
BseMII CTCAG 5 cut(s) 809, 1936, 2144, 2457, 2676
BseNI ACTGG 8 cut(s) 379, 1249, 1507, 1727, 1739, 2335, 2880, 3005
BseRI GAGGAG 4 cut(s) 91, 906, 1346, 1910
BseSI GKGCMC 4 cut(s) 1180, 1566, 1626, 1665
BseXI GCAGC 9 cut(s) 492, 1016, 1170, 1519, 1678, 1761, 1825, 1893, 2222
Bsh1236I CGCG 1 cut(s) 1033
Bsh1285I CGRYCG 1 cut(s) 2727
BshNI GGYRCC 2 cut(s) 2784, 2932
BshTI ACCGGT 1 cut(s) 1702
BshVI ATCGAT 1 cut(s) 3330
BsiEI CGRYCG 1 cut(s) 2727
BsiHKAI GWGCWC 1 cut(s) 682
BsiHKCI CYCGRG 1 cut(s) 1110
BsiSI CCGG 6 cut(s) 143, 150, 874, 1703, 2691, 2756
BslFI GGGAC 4 cut(s) 191, 420, 1749, 2862
BslI CCNNNNNNNGG 6 cut(s) 149, 409, 1244, 1330, 1733, 2942
BsmAI GTCTC 6 cut(s) 365, 1264, 2150, 2382, 2681, 2711
BsmFI GGGAC 4 cut(s) 191, 420, 1749, 2862
BsmI GAATGC 4 cut(s) 442, 1824, 2257, 2700
Bso31I GGTCTC 1 cut(s) 2681
BsoBI CYCGRG 1 cut(s) 1110
Bsp119I TTCGAA 2 cut(s) 2191, 2955
Bsp120I GGGCCC 3 cut(s) 1562, 1622, 1661
Bsp1286I GDGCHC 6 cut(s) 682, 1110, 1180, 1566, 1626, 1665
Bsp13I TCCGGA 1 cut(s) 2755
Bsp1407I TGTACA 1 cut(s) 1927
BspACI CCGC 7 cut(s) 316, 1033, 1436, 1506, 1943, 2300, 2487
BspCNI CTCAG 5 cut(s) 808, 1935, 2143, 2456, 2677
BspDI ATCGAT 1 cut(s) 3330
BspEI TCCGGA 1 cut(s) 2755
BspFNI CGCG 1 cut(s) 1033
BspHI TCATGA 1 cut(s) 960
BspMAI CTGCAG 1 cut(s) 2556
BspMI ACCTGC 2 cut(s) 1580, 1762
BspPI GGATC 8 cut(s) 65, 1163, 1229, 1846, 1859, 1940, 1953, 2766
BspQI GCTCTTC 1 cut(s) 2880
BspT104I TTCGAA 2 cut(s) 2191, 2955
BspT107I GGYRCC 2 cut(s) 2784, 2932
BspTI CTTAAG 1 cut(s) 497
BspTNI GGTCTC 1 cut(s) 2681
BsrBI CCGCTC 1 cut(s) 2489
BsrDI GCAATG 1 cut(s) 2181
BsrFI RCCGGY 2 cut(s) 873, 1702
BsrGI TGTACA 1 cut(s) 1927
BsrI ACTGG 8 cut(s) 379, 1249, 1507, 1727, 1739, 2335, 2880, 3005
BssAI RCCGGY 2 cut(s) 873, 1702
BssECI CCNNGG 8 cut(s) 403, 971, 1395, 1557, 1617, 1656, 1830, 2590
BssNAI GTATAC 1 cut(s) 3246
BssNI GRCGYC 1 cut(s) 1316
BssT1I CCWWGG 3 cut(s) 1395, 1830, 2590
Bst1107I GTATAC 1 cut(s) 3246
Bst2UI CCWGG 5 cut(s) 359, 404, 2304, 2937, 3212
Bst4CI ACNGT 8 cut(s) 90, 1022, 1721, 2041, 2065, 2188, 2376, 3027
Bst6I CTCTTC 6 cut(s) 1545, 1605, 1644, 2148, 2880, 2935
BstACI GRCGYC 1 cut(s) 1316
BstAFI CTTAAG 1 cut(s) 497
BstAUI TGTACA 1 cut(s) 1927
BstBI TTCGAA 2 cut(s) 2191, 2955
BstC8I GCNNGC 2 cut(s) 1163, 1779
BstDEI CTNAG 8 cut(s) 795, 1922, 2130, 2443, 2568, 2634, 2685, 3171
BstDSI CCRYGG 2 cut(s) 1557, 1656
BstEII GGTNACC 2 cut(s) 145, 1746
BstENI CCTNNNNNAGG 2 cut(s) 1328, 2940
BstFNI CGCG 1 cut(s) 1033
BstH2I RGCGCY 1 cut(s) 2137
BstHHI GCGC 2 cut(s) 2136, 3078
BstMAI GTCTC 6 cut(s) 365, 1264, 2150, 2382, 2681, 2711
BstMCI CGRYCG 1 cut(s) 2727
BstMWI GCNNNNNNNGC 7 cut(s) 446, 474, 1763, 2171, 2331, 3002, 3321
BstNI CCWGG 5 cut(s) 359, 404, 2304, 2937, 3212
BstNSI RCATGY 4 cut(s) 264, 1259, 2299, 3179
BstPI GGTNACC 2 cut(s) 145, 1746
BstSCI CCNGG 5 cut(s) 357, 402, 2302, 2935, 3210
BstSFI CTRYAG 2 cut(s) 2169, 2552
BstSLI GKGCMC 4 cut(s) 1180, 1566, 1626, 1665
BstUI CGCG 1 cut(s) 1033
BstV1I GCAGC 9 cut(s) 492, 1016, 1170, 1519, 1678, 1761, 1825, 1893, 2222
BstV2I GAAGAC 4 cut(s) 593, 1311, 1844, 2042
BstX2I RGATCY 2 cut(s) 1851, 1945
BstYI RGATCY 2 cut(s) 1851, 1945
BstZ17I GTATAC 1 cut(s) 3246
Bsu15I ATCGAT 1 cut(s) 3330
BsuI GTATCC 1 cut(s) 2117
BsuTUI ATCGAT 1 cut(s) 3330
BtgI CCRYGG 2 cut(s) 1557, 1656
BtgZI GCGATG 2 cut(s) 449, 1979
BtsI GCAGTG 2 cut(s) 999, 2907
BtsIMutI CAGTG 7 cut(s) 538, 999, 1734, 1746, 1930, 2887, 2907
BveI ACCTGC 2 cut(s) 1580, 1762
Cac8I GCNNGC 2 cut(s) 1163, 1779
CaiI CAGNNNCTG 1 cut(s) 374
CciI TCATGA 1 cut(s) 960
CfoI GCGC 2 cut(s) 2136, 3078
Cfr10I RCCGGY 2 cut(s) 873, 1702
ClaI ATCGAT 1 cut(s) 3330
CseI GACGC 2 cut(s) 1305, 1849
Csp6I GTAC 9 cut(s) 132, 968, 1023, 1928, 2098, 2605, 2669, 2785, 2986
CspAI ACCGGT 1 cut(s) 1702
CviQI GTAC 9 cut(s) 132, 968, 1023, 1928, 2098, 2605, 2669, 2785, 2986
DdeI CTNAG 8 cut(s) 795, 1922, 2130, 2443, 2568, 2634, 2685, 3171
DraI TTTAAA 1 cut(s) 1213
DrdI GACNNNNNNGTC 1 cut(s) 727
DseDI GACNNNNNNGTC 1 cut(s) 727
EaeI YGGCCR 1 cut(s) 567
Eam1104I CTCTTC 6 cut(s) 1545, 1605, 1644, 2148, 2880, 2935
EarI CTCTTC 6 cut(s) 1545, 1605, 1644, 2148, 2880, 2935
EciI GGCGGA 3 cut(s) 331, 1451, 1958
Ecl136II GAGCTC 1 cut(s) 680
Eco130I CCWWGG 3 cut(s) 1395, 1830, 2590
Eco24I GRGCYC 5 cut(s) 682, 1110, 1566, 1626, 1665
Eco31I GGTCTC 1 cut(s) 2681
Eco32I GATATC 2 cut(s) 2921, 3334
Eco47I GGWCC 7 cut(s) 361, 650, 975, 1511, 1724, 1736, 2587
Eco47III AGCGCT 1 cut(s) 2135
Eco53kI GAGCTC 1 cut(s) 680
Eco57I CTGAAG 3 cut(s) 758, 1310, 1916
Eco88I CYCGRG 1 cut(s) 1110
Eco91I GGTNACC 2 cut(s) 145, 1746
EcoICRI GAGCTC 1 cut(s) 680
EcoNI CCTNNNNNAGG 2 cut(s) 1328, 2940
EcoO109I RGGNCCY 9 cut(s) 650, 1562, 1563, 1622, 1623, 1661, 1662, 2548, 2587
EcoO65I GGTNACC 2 cut(s) 145, 1746
EcoRI GAATTC 1 cut(s) 885
EcoRII CCWGG 5 cut(s) 357, 402, 2302, 2935, 3210
EcoRV GATATC 2 cut(s) 2921, 3334
EcoT14I CCWWGG 3 cut(s) 1395, 1830, 2590
EcoT22I ATGCAT 1 cut(s) 2874
EcoT38I GRGCYC 5 cut(s) 682, 1110, 1566, 1626, 1665
ErhI CCWWGG 3 cut(s) 1395, 1830, 2590
FalI AAGNNNNNCTT 2 cut(s) 1175, 1207
FaqI GGGAC 4 cut(s) 191, 420, 1749, 2862
FauI CCCGC 1 cut(s) 1026
FauNDI CATATG 1 cut(s) 60
FbaI TGATCA 2 cut(s) 957, 2178
FblI GTMKAC 3 cut(s) 2470, 3029, 3245
FriOI GRGCYC 5 cut(s) 682, 1110, 1566, 1626, 1665
FspBI CTAG 8 cut(s) 75, 251, 257, 1467, 1979, 2382, 2795, 2826
GlaI GCGC 2 cut(s) 2135, 3077
GsuI CTGGAG 3 cut(s) 396, 696, 1804
HaeII RGCGCY 1 cut(s) 2137
HapII CCGG 6 cut(s) 143, 150, 874, 1703, 2691, 2756
HgaI GACGC 2 cut(s) 1305, 1849
HhaI GCGC 2 cut(s) 2136, 3078
Hin1I GRCGYC 1 cut(s) 1316
Hin6I GCGC 2 cut(s) 2134, 3076
HinP1I GCGC 2 cut(s) 2134, 3076
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HindIII AAGCTT 2 cut(s) 1064, 1163
HpaI GTTAAC 1 cut(s) 183
HpaII CCGG 6 cut(s) 143, 150, 874, 1703, 2691, 2756
HphI GGTGA 6 cut(s) 585, 1048, 1740, 1846, 2978, 3020
HpyCH4III ACNGT 8 cut(s) 90, 1022, 1721, 2041, 2065, 2188, 2376, 3027
HpyCH4IV ACGT 4 cut(s) 179, 695, 2659, 2791
HpyF10VI GCNNNNNNNGC 7 cut(s) 446, 474, 1763, 2171, 2331, 3002, 3321
HpyF3I CTNAG 8 cut(s) 795, 1922, 2130, 2443, 2568, 2634, 2685, 3171
HpySE526I ACGT 4 cut(s) 179, 695, 2659, 2791
Hsp92I GRCGYC 1 cut(s) 1316
HspAI GCGC 2 cut(s) 2134, 3076
Kpn2I TCCGGA 1 cut(s) 2755
KpnI GGTACC 1 cut(s) 2788
Ksp22I TGATCA 2 cut(s) 957, 2178
KspAI GTTAAC 1 cut(s) 183
LguI GCTCTTC 1 cut(s) 2880
LmnI GCTCC 4 cut(s) 677, 1105, 1718, 2085
Lsp1109I GCAGC 9 cut(s) 492, 1016, 1170, 1519, 1678, 1761, 1825, 1893, 2222
MaeI CTAG 8 cut(s) 75, 251, 257, 1467, 1979, 2382, 2795, 2826
MaeII ACGT 4 cut(s) 179, 695, 2659, 2791
MaeIII GTNAC 9 cut(s) 145, 268, 551, 691, 876, 1746, 2014, 2518, 3067
MbiI CCGCTC 1 cut(s) 2489
MfeI CAATTG 1 cut(s) 2597
MflI RGATCY 2 cut(s) 1851, 1945
MhlI GDGCHC 6 cut(s) 682, 1110, 1180, 1566, 1626, 1665
MlsI TGGCCA 1 cut(s) 569
MluNI TGGCCA 1 cut(s) 569
MlyI GAGTC 4 cut(s) 185, 304, 1951, 2316
MmeI TCCRAC 6 cut(s) 161, 1396, 1740, 2008, 2023, 3034
Mox20I TGGCCA 1 cut(s) 569
Mph1103I ATGCAT 1 cut(s) 2874
MroI TCCGGA 1 cut(s) 2755
MroXI GAANNNNTTC 2 cut(s) 1423, 2255
MscI TGGCCA 1 cut(s) 569
MslI CAYNNNNRTG 5 cut(s) 259, 639, 716, 815, 2895
Msp20I TGGCCA 1 cut(s) 569
MspCI CTTAAG 1 cut(s) 497
MspI CCGG 6 cut(s) 143, 150, 874, 1703, 2691, 2756
MspR9I CCNGG 5 cut(s) 359, 404, 2304, 2937, 3212
MunI CAATTG 1 cut(s) 2597
Mva1269I GAATGC 4 cut(s) 442, 1824, 2257, 2700
MvaI CCWGG 5 cut(s) 359, 404, 2304, 2937, 3212
MvnI CGCG 1 cut(s) 1033
MwoI GCNNNNNNNGC 7 cut(s) 446, 474, 1763, 2171, 2331, 3002, 3321
NdeI CATATG 1 cut(s) 60
NmeAIII GCCGAG 2 cut(s) 292, 1642
NmuCI GTSAC 6 cut(s) 268, 551, 876, 1746, 2518, 3067
NsiI ATGCAT 1 cut(s) 2874
NspI RCATGY 4 cut(s) 264, 1259, 2299, 3179
NspV TTCGAA 2 cut(s) 2191, 2955
PagI TCATGA 1 cut(s) 960
PaqCI CACCTGC 2 cut(s) 1580, 1762
PciI ACATGT 1 cut(s) 1255
PciSI GCTCTTC 1 cut(s) 2880
PctI GAATGC 4 cut(s) 442, 1824, 2257, 2700
PdmI GAANNNNTTC 2 cut(s) 1423, 2255
PfeI GAWTC 9 cut(s) 319, 536, 1051, 1283, 1361, 1400, 1900, 2193, 2400
PinAI ACCGGT 1 cut(s) 1702
Ple19I CGATCG 1 cut(s) 2727
PleI GAGTC 4 cut(s) 185, 304, 1951, 2315
PpsI GAGTC 4 cut(s) 185, 304, 1951, 2315
PpuMI RGGWCCY 2 cut(s) 650, 2587
PscI ACATGT 1 cut(s) 1255
Psp124BI GAGCTC 1 cut(s) 682
Psp1406I AACGTT 1 cut(s) 695
Psp5II RGGWCCY 2 cut(s) 650, 2587
Psp6I CCWGG 5 cut(s) 357, 402, 2302, 2935, 3210
PspEI GGTNACC 2 cut(s) 145, 1746
PspGI CCWGG 5 cut(s) 357, 402, 2302, 2935, 3210
PspOMI GGGCCC 3 cut(s) 1562, 1622, 1661
PspPPI RGGWCCY 2 cut(s) 650, 2587
PstI CTGCAG 1 cut(s) 2556
PstNI CAGNNNCTG 1 cut(s) 374
PsuI RGATCY 2 cut(s) 1851, 1945
PvuI CGATCG 1 cut(s) 2727
RsaI GTAC 9 cut(s) 133, 969, 1024, 1929, 2099, 2606, 2670, 2786, 2987
RsaNI GTAC 9 cut(s) 132, 968, 1023, 1928, 2098, 2605, 2669, 2785, 2986
RseI CAYNNNNRTG 5 cut(s) 259, 639, 716, 815, 2895
SacI GAGCTC 1 cut(s) 682
SapI GCTCTTC 1 cut(s) 2880
ScaI AGTACT 1 cut(s) 2606
SchI GAGTC 4 cut(s) 185, 304, 1951, 2316
ScrFI CCNGG 5 cut(s) 359, 404, 2304, 2937, 3212
SduI GDGCHC 6 cut(s) 682, 1110, 1180, 1566, 1626, 1665
SfcI CTRYAG 2 cut(s) 2169, 2552
SfuI TTCGAA 2 cut(s) 2191, 2955
SinI GGWCC 7 cut(s) 361, 650, 975, 1511, 1724, 1736, 2587
SmiMI CAYNNNNRTG 5 cut(s) 259, 639, 716, 815, 2895
SmlI CTYRAG 2 cut(s) 497, 1959
SmoI CTYRAG 2 cut(s) 497, 1959
SsiI CCGC 7 cut(s) 316, 1033, 1436, 1506, 1943, 2300, 2487
SspI AATATT 1 cut(s) 112
SspMI CTAG 8 cut(s) 75, 251, 257, 1467, 1979, 2382, 2795, 2826
SstI GAGCTC 1 cut(s) 682
StyD4I CCNGG 5 cut(s) 357, 402, 2302, 2935, 3210
StyI CCWWGG 3 cut(s) 1395, 1830, 2590
TaaI ACNGT 8 cut(s) 90, 1022, 1721, 2041, 2065, 2188, 2376, 3027
TaiI ACGT 4 cut(s) 182, 698, 2662, 2794
TaqI TCGA 8 cut(s) 715, 1357, 1455, 2191, 2727, 2955, 3190, 3330
TaqII GACCGA 1 cut(s) 963
TatI WGTACW 3 cut(s) 1927, 2604, 2668
TauI GCSGC 1 cut(s) 2302
TfiI GAWTC 9 cut(s) 319, 536, 1051, 1283, 1361, 1400, 1900, 2193, 2400
TscAI CASTG 7 cut(s) 538, 999, 1734, 1746, 1930, 2887, 2907
TseFI GTSAC 6 cut(s) 268, 551, 876, 1746, 2518, 3067
TseI GCWGC 9 cut(s) 505, 1004, 1158, 1532, 1691, 1774, 1813, 1906, 2210
Tsp45I GTSAC 6 cut(s) 268, 551, 876, 1746, 2518, 3067
TspGWI ACGGA 2 cut(s) 270, 955
TspRI CASTG 7 cut(s) 538, 999, 1734, 1746, 1930, 2887, 2907
Vha464I CTTAAG 1 cut(s) 497
VpaK11BI GGWCC 7 cut(s) 361, 650, 975, 1511, 1724, 1736, 2587
XagI CCTNNNNNAGG 2 cut(s) 1328, 2940
XapI RAATTY 8 cut(s) 630, 660, 773, 885, 1208, 1376, 1420, 2573
XceI RCATGY 4 cut(s) 264, 1259, 2299, 3179
XcmI CCANNNNNNNNNTGG 1 cut(s) 1099
XmiI GTMKAC 3 cut(s) 2470, 3029, 3245
XmnI GAANNNNTTC 2 cut(s) 1423, 2255
XspI CTAG 8 cut(s) 75, 251, 257, 1467, 1979, 2382, 2795, 2826
ZrmI AGTACT 1 cut(s) 2606
Zsp2I ATGCAT 1 cut(s) 2874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.