Reverse transcriptase (RNA-dependent DNA polymerase)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 13 |
| AciI | CCGC | 1 cut(s) | 542 |
| AcoI | YGGCCR | 2 cut(s) | 134, 405 |
| AcsI | RAATTY | 1 cut(s) | 475 |
| AcuI | CTGAAG | 1 cut(s) | 441 |
| AfaI | GTAC | 1 cut(s) | 59 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 96, 248 |
| AgsI | TTSAA | 1 cut(s) | 264 |
| AjnI | CCWGG | 2 cut(s) | 241, 407 |
| AluBI | AGCT | 2 cut(s) | 71, 206 |
| AluI | AGCT | 2 cut(s) | 71, 206 |
| AoxI | GGCC | 3 cut(s) | 134, 309, 405 |
| ApeKI | GCWGC | 2 cut(s) | 539, 575 |
| ApoI | RAATTY | 1 cut(s) | 475 |
| AseI | ATTAAT | 1 cut(s) | 113 |
| AspS9I | GGNCC | 1 cut(s) | 309 |
| BalI | TGGCCA | 1 cut(s) | 407 |
| BbvI | GCAGC | 2 cut(s) | 551, 587 |
| BciT130I | CCWGG | 2 cut(s) | 243, 409 |
| BciVI | GTATCC | 1 cut(s) | 629 |
| BclI | TGATCA | 1 cut(s) | 210 |
| BfaI | CTAG | 2 cut(s) | 5, 278 |
| BfuI | GTATCC | 1 cut(s) | 629 |
| BisI | GCNGC | 2 cut(s) | 540, 576 |
| BlsI | GCNGC | 2 cut(s) | 541, 577 |
| Bme1390I | CCNGG | 2 cut(s) | 243, 409 |
| BmgT120I | GGNCC | 1 cut(s) | 309 |
| BmiI | GGNNCC | 1 cut(s) | 87 |
| BmrFI | CCNGG | 2 cut(s) | 243, 409 |
| BmrI | ACTGGG | 1 cut(s) | 316 |
| BmsI | GCATC | 1 cut(s) | 289 |
| BmuI | ACTGGG | 1 cut(s) | 316 |
| BsaJI | CCNNGG | 1 cut(s) | 513 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 96, 248 |
| Bse118I | RCCGGY | 1 cut(s) | 311 |
| Bse1I | ACTGG | 2 cut(s) | 54, 311 |
| Bse3DI | GCAATG | 1 cut(s) | 141 |
| BseBI | CCWGG | 2 cut(s) | 243, 409 |
| BseDI | CCNNGG | 1 cut(s) | 513 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 96, 248 |
| BseMI | GCAATG | 1 cut(s) | 141 |
| BseMII | CTCAG | 1 cut(s) | 212 |
| BseNI | ACTGG | 2 cut(s) | 54, 311 |
| BseRI | GAGGAG | 1 cut(s) | 595 |
| BseXI | GCAGC | 2 cut(s) | 551, 587 |
| BsgI | GTGCAG | 1 cut(s) | 222 |
| BshFI | GGCC | 3 cut(s) | 136, 311, 407 |
| BsiSI | CCGG | 1 cut(s) | 312 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 96, 248 |
| BsnI | GGCC | 3 cut(s) | 136, 311, 407 |
| Bsp143I | GATC | 1 cut(s) | 210 |
| Bsp19I | CCATGG | 1 cut(s) | 513 |
| BspACI | CCGC | 1 cut(s) | 542 |
| BspANI | GGCC | 3 cut(s) | 136, 311, 407 |
| BspCNI | CTCAG | 1 cut(s) | 213 |
| BspLI | GGNNCC | 1 cut(s) | 87 |
| BsrDI | GCAATG | 1 cut(s) | 141 |
| BsrFI | RCCGGY | 1 cut(s) | 311 |
| BsrI | ACTGG | 2 cut(s) | 54, 311 |
| BssAI | RCCGGY | 1 cut(s) | 311 |
| BssECI | CCNNGG | 1 cut(s) | 513 |
| BssMI | GATC | 1 cut(s) | 210 |
| BssT1I | CCWWGG | 1 cut(s) | 513 |
| Bst2UI | CCWGG | 2 cut(s) | 243, 409 |
| BstDEI | CTNAG | 1 cut(s) | 221 |
| BstDSI | CCRYGG | 1 cut(s) | 513 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 94, 246 |
| BstKTI | GATC | 1 cut(s) | 213 |
| BstMBI | GATC | 1 cut(s) | 210 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 308 |
| BstNI | CCWGG | 2 cut(s) | 243, 409 |
| BstSCI | CCNGG | 2 cut(s) | 241, 407 |
| BstV1I | GCAGC | 2 cut(s) | 551, 587 |
| BsuI | GTATCC | 1 cut(s) | 629 |
| BsuRI | GGCC | 3 cut(s) | 136, 311, 407 |
| BtgI | CCRYGG | 1 cut(s) | 513 |
| Cfr10I | RCCGGY | 1 cut(s) | 311 |
| Cfr13I | GGNCC | 1 cut(s) | 309 |
| Csp6I | GTAC | 1 cut(s) | 58 |
| CviAII | CATG | 3 cut(s) | 175, 446, 514 |
| CviQI | GTAC | 1 cut(s) | 58 |
| DdeI | CTNAG | 1 cut(s) | 221 |
| DpnI | GATC | 1 cut(s) | 212 |
| DpnII | GATC | 1 cut(s) | 210 |
| DraI | TTTAAA | 2 cut(s) | 231, 499 |
| EaeI | YGGCCR | 2 cut(s) | 134, 405 |
| Eco130I | CCWWGG | 1 cut(s) | 513 |
| Eco57I | CTGAAG | 1 cut(s) | 441 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 94, 246 |
| EcoRII | CCWGG | 2 cut(s) | 241, 407 |
| EcoT14I | CCWWGG | 1 cut(s) | 513 |
| EcoT22I | ATGCAT | 1 cut(s) | 180 |
| ErhI | CCWWGG | 1 cut(s) | 513 |
| FaeI | CATG | 3 cut(s) | 178, 449, 517 |
| FatI | CATG | 3 cut(s) | 174, 445, 513 |
| FauNDI | CATATG | 2 cut(s) | 148, 198 |
| FbaI | TGATCA | 1 cut(s) | 210 |
| FblI | GTMKAC | 1 cut(s) | 13 |
| Fnu4HI | GCNGC | 2 cut(s) | 540, 576 |
| Fsp4HI | GCNGC | 2 cut(s) | 540, 576 |
| FspBI | CTAG | 2 cut(s) | 5, 278 |
| GluI | GCNGC | 2 cut(s) | 540, 576 |
| HaeIII | GGCC | 3 cut(s) | 136, 311, 407 |
| HapII | CCGG | 1 cut(s) | 312 |
| Hin1II | CATG | 3 cut(s) | 178, 449, 517 |
| HinfI | GANTC | 1 cut(s) | 166 |
| HpaII | CCGG | 1 cut(s) | 312 |
| Hpy166II | GTNNAC | 1 cut(s) | 14 |
| Hpy188I | TCNGA | 2 cut(s) | 96, 222 |
| Hpy188III | TCNNGA | 2 cut(s) | 278, 485 |
| Hpy8I | GTNNAC | 1 cut(s) | 14 |
| HpyAV | CCTTC | 1 cut(s) | 76 |
| HpyCH4V | TGCA | 5 cut(s) | 146, 152, 178, 239, 302 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 308 |
| HpyF3I | CTNAG | 1 cut(s) | 221 |
| Hsp92II | CATG | 3 cut(s) | 178, 449, 517 |
| Ksp22I | TGATCA | 1 cut(s) | 210 |
| Kzo9I | GATC | 1 cut(s) | 210 |
| LmnI | GCTCC | 1 cut(s) | 91 |
| Lsp1109I | GCAGC | 2 cut(s) | 551, 587 |
| LweI | GCATC | 1 cut(s) | 289 |
| MaeI | CTAG | 2 cut(s) | 5, 278 |
| MaeIII | GTNAC | 3 cut(s) | 319, 344, 373 |
| MalI | GATC | 1 cut(s) | 212 |
| MboI | GATC | 1 cut(s) | 210 |
| MlsI | TGGCCA | 1 cut(s) | 407 |
| MluCI | AATT | 5 cut(s) | 170, 458, 475, 609, 637 |
| MluNI | TGGCCA | 1 cut(s) | 407 |
| MlyI | GAGTC | 1 cut(s) | 175 |
| MnlI | CCTC | 6 cut(s) | 90, 216, 428, 589, 613, 616 |
| Mox20I | TGGCCA | 1 cut(s) | 407 |
| Mph1103I | ATGCAT | 1 cut(s) | 180 |
| MscI | TGGCCA | 1 cut(s) | 407 |
| MseI | TTAA | 3 cut(s) | 113, 230, 498 |
| Msp20I | TGGCCA | 1 cut(s) | 407 |
| MspA1I | CMGCKG | 1 cut(s) | 542 |
| MspI | CCGG | 1 cut(s) | 312 |
| MspR9I | CCNGG | 2 cut(s) | 243, 409 |
| MvaI | CCWGG | 2 cut(s) | 243, 409 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 308 |
| NcoI | CCATGG | 1 cut(s) | 513 |
| NdeI | CATATG | 2 cut(s) | 148, 198 |
| NdeII | GATC | 1 cut(s) | 210 |
| NlaIII | CATG | 3 cut(s) | 178, 449, 517 |
| NlaIV | GGNNCC | 1 cut(s) | 87 |
| NmeAIII | GCCGAG | 1 cut(s) | 112 |
| NsiI | ATGCAT | 1 cut(s) | 180 |
| PkrI | GCNGC | 2 cut(s) | 541, 577 |
| PleI | GAGTC | 1 cut(s) | 174 |
| PpsI | GAGTC | 1 cut(s) | 174 |
| PshBI | ATTAAT | 1 cut(s) | 113 |
| Psp6I | CCWGG | 2 cut(s) | 241, 407 |
| PspGI | CCWGG | 2 cut(s) | 241, 407 |
| PspN4I | GGNNCC | 1 cut(s) | 87 |
| PspPI | GGNCC | 1 cut(s) | 309 |
| RsaI | GTAC | 1 cut(s) | 59 |
| RsaNI | GTAC | 1 cut(s) | 58 |
| SaqAI | TTAA | 3 cut(s) | 113, 230, 498 |
| SatI | GCNGC | 2 cut(s) | 540, 576 |
| Sau3AI | GATC | 1 cut(s) | 210 |
| Sau96I | GGNCC | 1 cut(s) | 309 |
| SchI | GAGTC | 1 cut(s) | 175 |
| ScrFI | CCNGG | 2 cut(s) | 243, 409 |
| SfaNI | GCATC | 1 cut(s) | 289 |
| Sse9I | AATT | 5 cut(s) | 170, 458, 475, 609, 637 |
| SsiI | CCGC | 1 cut(s) | 542 |
| SspMI | CTAG | 2 cut(s) | 5, 278 |
| StyD4I | CCNGG | 2 cut(s) | 241, 407 |
| StyI | CCWWGG | 1 cut(s) | 513 |
| TasI | AATT | 5 cut(s) | 170, 458, 475, 609, 637 |
| Tru1I | TTAA | 3 cut(s) | 113, 230, 498 |
| Tru9I | TTAA | 3 cut(s) | 113, 230, 498 |
| TseI | GCWGC | 2 cut(s) | 539, 575 |
| TspDTI | ATGAA | 2 cut(s) | 163, 215 |
| TspGWI | ACGGA | 1 cut(s) | 338 |
| VspI | ATTAAT | 1 cut(s) | 113 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 94, 246 |
| XapI | RAATTY | 1 cut(s) | 475 |
| XbaI | TCTAGA | 1 cut(s) | 277 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 511 |
| XmiI | GTMKAC | 1 cut(s) | 13 |
| XspI | CTAG | 2 cut(s) | 5, 278 |
| Zsp2I | ATGCAT | 1 cut(s) | 180 |