RchiOBHm_Chr2g0124031

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000071
Physical Location & Seq
Forward (+)
37382766 .. 37387326
4561 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49627

Sequence Viewer

Length: 2844 bp
ATGTTAGTTAAAGGTCAGAGTTCCAAGGTCAATGATGTCCAGAAGGTTGAGGTGTCTGTGCGAAGTTCAGATGGAGGTTCTTTTGGGGATTGTGGTAAGATTGGTATGGCTTTAAATGTTTCTAACTTTGTTGGTTCTGATACATGGATTATTGATTCTGGTGCCTCCGATCATATGACTTATGATAAATCATATTTTACTGACTTGTCTTCCCCACCAGTGTCCTATGTCACCAATGCCAATGGTGAGGCTTTTCCTGTGTTAGGGTCCGGGTCAGTGCGTGTTACTCCCACTTTAGAGCTTCATAATGTGTTATATGTACCTGACTTGTCTCATCACTTGATATCTGTTCCACAGTTGAATACTGAGTCTAAATGCTCCGTAACCTTTTATCCTATGTATGTGATCTTTCAGGATCTTCTCACCAGGGAGATAGTCGGTCGGGGGTATCTGAGGGTCAGGTTGTTCCATCTGGATCAGACATACGCTGGAGAGAAACCAGGAGCACAGTCCCGAGTCGCGTTGACTTCGAATTCTGATAAGATAAGTGAAATTTGGTTGTGGCATCGCCATTTAGGGCATCATTCTTTTAGTCTTATGAAAACAACCATGCCTACTCTGTTTATTGGTGTGAATGAGTCTGCTTTACGTTGTGAAACATATGTTTTGGCCAAGAGTCATCGTGCTACTTATTCTCCTAGAGTGTCTAATAAAAGTGTTATTCCTTTTGAGTTGATTCATTCTGATGTTTGGGGACCTTCTAGAGAGCCCACTGTATCTGGTATGAGATACAACTCTTCAGGGGAGAATTCATTTTTTGAAGAGCTGTATCATGGAGAGGGGGAGGAATCAGAAGGAGGAGAAGAAGCAAGTGGTATTTTGACAGACCCAGTTGAGACCATTAGCTCGTCACCAGCAGAAGCTCCAAACATCCAAGCACCAGTTTCTATGGCCGAAAATGATGTTGAAGATACAACTCCAAACATTCAAGCACCAGTCTCTATGGCCGAAAATGATGTTGAAGACACAACTGTCCCTCCTACCATTGCTTCTACCCCTGACCCACTGCTTCCTGGTACTGAAGATCACTCATTTGAGGTATGTCCACCCACTAGTACTAGTAGTAATGAGTCTACTGTTGGGCAATATGTGTTACCAAATAGGACCACTCGGGGTCAATCAGTCAAAAGATATGAACCTACCCTTACTGCCAAATCAAAATACCCAGTAGCCAATTATATGTCCACTAGGAGGTTGTCTAAGTCATATGAATCTTTTATGAATCAAATATCTGCTGTATCAGTACCTAACAAAGTGCAGGATGCATTGGGGGATCCAAAGTGGAGGAAGGCAATGGAGGAAGAGATGGAGGCGTTGCAGAAGAGCAATACTTGGCAACTTGTGCCTCCACCACAAGGCAAGAAGGCTGTAGGTTGTCGTTGGGTGTTTACCGTGAAGCATAATGCAGATGGATCAGTGAATCGGTACAAAGCACGTCTTGTAGCAAAAGGGTTTACTCAGACGTATGGTATTGACTACGATGAAACATTTGCCCCTGTTGCCAAGATGAACACTATTCGGGTTTTGCTCTCATTCGCTGCTAGTTTAAACTGGCCACTCCGACAGTTTGATGTCAAGAATGCATTTCTTCATGGAGAGTTAGCCGAGGAAGTGTACATGAGCCTTCCACTTGGGTATGTAGTTGCCTCTCCTGGTGATTTTGTATGCAGATTGAGAAAATCTCTGTATGGTCTTAAACAGTCACCTCGTGCTTGGTTTGGAAGATTTTCACAGTTCATGCGGAAGGTTGGTTACAGACAGAGCAACTCAGACCATACCTTGTTCCTTAAGCATCAACAAGGGAAAGTAACAGCTCTAATTATTTATGTGGATGATATGATAATCACTGGCAATGATACTGTTGAGATAGATAGACTGCAGAGACAGCTAGCCTTTGAGTTTGAGATGAAGGACTTGGGTGAACTTAAGTACTTCTTAGGAATTGAGGTAGCCAGGGGGAGAGAAGGAATCTACCTGTGTCAGAGGAAGTACGTTCTTGACTTGCTGACAAAGACAGGTTTGTTGGATTGCAGACCTGTTGATACTCCTATTGAGCAGAACCATTGTTTAGCTGAGTATCCAGATCAGGTACCTACTGATCGAGCTCGTTATCAGAGGTTGGTTGGGCGCTTGATTTATTTGGCTCATACTAGACCAGATGTTGCATATGCAGTGAGTGTGGTGAGTCAGTTCATGCATAACCCGAGTGAGAGTCATATGGATGCTGTTATGCGAATTTTGAAGTACTTAAAGTCAGCTCCAGGAATGGGAGTACTGTTTTCTAAACACAACAACATTCTTGATGTTTGTGGCTTCACAGATGCAGATTGGGCTGGAAATATTACAGACAGGAGGTCAACATCAGGTTACTTTACCTTTGTGGGAGGTAATTTGGTTACATGGAAGAGTAAGAAACAGAAAGTTGTGGCACGATCTAGTGCTGAGGCTGAGTATAGGGGTATGGCTCAAGGAGTGTGTGAATTGTTGTGGTTGAGAAATTTGTTACGTGATTTGGGTTTCAAACTCAAGAGTACCATGCAGTTGTATTGTGACAACAAGGCAGCCATTGACATATCACAGAATCCAGTACAGCATGATCGTACTAAGCATGTCGAGGTTGATCGTCACTTTATAAAGGAGAAGCTAGATGCCAAAATTATTAGTTTTCCTTTTGTTCCAACTGAAGAGCAACTTGCAGATGTACTTACCAAAGGAGTTTCCAGGAAGGCGTTTTATGACTCACTAAGCAAGTTGGGCATGGTTGATGTATATGCGCCAACTTGA

Protein Analysis

947

Amino Acids

105.97

Weight (kDa)

6.6

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 49 - 122 2.2e-19 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 171 - 226 2.9e-09 GAG-pre-integrase domain
RVT_2 PF07727 463 - 705 8.6e-75 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2693
AasI GACNNNNNNGTC 1 cut(s) 1031
Acc65I GGTACC 1 cut(s) 2149
AccB1I GGYRCC 2 cut(s) 161, 2149
AccI GTMKAC 1 cut(s) 1133
AccII CGCG 1 cut(s) 521
AciI CCGC 1 cut(s) 1801
AclWI GGATC 5 cut(s) 423, 483, 1328, 1341, 1480
AcoI YGGCCR 4 cut(s) 669, 951, 1005, 1613
AcsI RAATTY 5 cut(s) 532, 552, 808, 2295, 2557
AcuI CTGAAG 3 cut(s) 783, 1101, 2763
AdeI CACNNNGTG 1 cut(s) 1769
AfiI CCNNNNNNNGG 4 cut(s) 263, 1251, 1415, 2327
AflII CTTAAG 2 cut(s) 1847, 1985
AgsI TTSAA 7 cut(s) 361, 821, 968, 989, 1022, 2302, 2581
AhdI GACNNNNNGTC 1 cut(s) 2413
AhlI ACTAGT 2 cut(s) 1112, 1118
AjiI CACGTC 1 cut(s) 1496
AjnI CCWGG 7 cut(s) 425, 499, 1072, 1711, 2012, 2320, 2780
Alw21I GWGCWC 2 cut(s) 508, 2167
Alw26I GTCTC 4 cut(s) 336, 890, 1003, 1936
AlwI GGATC 5 cut(s) 423, 483, 1328, 1341, 1480
Ama87I CYCGRG 3 cut(s) 513, 1170, 2263
AoxI GGCC 4 cut(s) 669, 951, 1005, 1613
ApeKI GCWGC 2 cut(s) 1598, 2621
ApoI RAATTY 5 cut(s) 532, 552, 808, 2295, 2557
Asp700I GAANNNNTTC 1 cut(s) 1786
Asp718I GGTACC 1 cut(s) 2149
AspLEI GCGC 2 cut(s) 2190, 2836
AspS9I GGNCC 3 cut(s) 267, 755, 1164
AsuC2I CCSGG 1 cut(s) 271
AsuHPI GGTGA 8 cut(s) 223, 257, 415, 903, 1727, 1755, 1991, 2254
AsuII TTCGAA 1 cut(s) 530
AsuNHI GCTAGC 1 cut(s) 1948
AvaI CYCGRG 3 cut(s) 513, 1170, 2263
AvaII GGWCC 3 cut(s) 267, 755, 1164
BalI TGGCCA 2 cut(s) 671, 1615
BamHI GGATCC 1 cut(s) 1333
BanI GGYRCC 2 cut(s) 161, 2149
BanII GRGCYC 2 cut(s) 771, 2167
BarI GAAGNNNNNNTAC 2 cut(s) 1796, 1828
BauI CACGAG 1 cut(s) 1767
BbsI GAAGAC 2 cut(s) 201, 1029
Bbv12I GWGCWC 2 cut(s) 508, 2167
BbvCI CCTCAGC 1 cut(s) 2502
BbvI GCAGC 2 cut(s) 1585, 2633
BccI CCATC 4 cut(s) 65, 477, 1360, 1463
BciT130I CCWGG 7 cut(s) 427, 501, 1074, 1713, 2014, 2322, 2782
BciVI GTATCC 1 cut(s) 2148
BcnI CCSGG 1 cut(s) 271
BcoDI GTCTC 4 cut(s) 336, 890, 1003, 1936
BcuI ACTAGT 2 cut(s) 1112, 1118
BfmI CTRYAG 2 cut(s) 1428, 1937
BfoI RGCGCY 1 cut(s) 2191
BfrI CTTAAG 2 cut(s) 1847, 1985
BfuI GTATCC 1 cut(s) 2148
BisI GCNGC 2 cut(s) 1599, 2622
BlsI GCNGC 2 cut(s) 1600, 2623
BmcAI AGTACT 4 cut(s) 1117, 1991, 2306, 2334
Bme1390I CCNGG 8 cut(s) 271, 427, 501, 1074, 1713, 2014, 2322, 2782
Bme18I GGWCC 3 cut(s) 267, 755, 1164
BmeRI GACNNNNNGTC 1 cut(s) 2413
BmeT110I CYCGRG 3 cut(s) 513, 1170, 2263
BmgBI CACGTC 1 cut(s) 1496
BmgT120I GGNCC 3 cut(s) 267, 755, 1164
BmiI GGNNCC 5 cut(s) 163, 268, 756, 1335, 2151
BmrFI CCNGG 8 cut(s) 271, 427, 501, 1074, 1713, 2014, 2322, 2782
BmrI ACTGGG 2 cut(s) 884, 1220
BmsI GCATC 7 cut(s) 574, 589, 1312, 1861, 2272, 2371, 2698
BmtI GCTAGC 1 cut(s) 1952
BmuI ACTGGG 2 cut(s) 884, 1220
BpiI GAAGAC 2 cut(s) 201, 1029
BplI GAGNNNNNCTC 2 cut(s) 1726, 1758
BpmI CTGGAG 2 cut(s) 510, 2304
Bpu10I CCTNAGC 1 cut(s) 2502
Bpu14I TTCGAA 1 cut(s) 530
BpuEI CTTGAG 2 cut(s) 2511, 2570
BpuMI CCSGG 1 cut(s) 271
BsaAI YACGTR 1 cut(s) 2567
BsaI GGTCTC 1 cut(s) 890
BsaJI CCNNGG 4 cut(s) 24, 426, 1665, 2013
BsaXI ACNNNNNCTCC 4 cut(s) 679, 709, 1021, 1051
Bsc4I CCNNNNNNNGG 4 cut(s) 263, 1251, 1415, 2327
Bse1I ACTGG 8 cut(s) 218, 890, 941, 995, 1226, 1616, 1912, 2645
Bse3DI GCAATG 3 cut(s) 1044, 1359, 1918
BseBI CCWGG 7 cut(s) 427, 501, 1074, 1713, 2014, 2322, 2782
BseDI CCNNGG 4 cut(s) 24, 426, 1665, 2013
BseGI GGATG 4 cut(s) 930, 1327, 1897, 2287
BseLI CCNNNNNNNGG 4 cut(s) 263, 1251, 1415, 2327
BseMI GCAATG 3 cut(s) 1044, 1359, 1918
BseMII CTCAG 7 cut(s) 357, 443, 1532, 1842, 2124, 2493, 2499
BseNI ACTGG 8 cut(s) 218, 890, 941, 995, 1226, 1616, 1912, 2645
BseRI GAGGAG 1 cut(s) 873
BseXI GCAGC 2 cut(s) 1585, 2633
BsgI GTGCAG 1 cut(s) 1337
Bsh1236I CGCG 1 cut(s) 521
Bsh1285I CGRYCG 1 cut(s) 442
BshFI GGCC 4 cut(s) 671, 953, 1007, 1615
BshNI GGYRCC 2 cut(s) 161, 2149
BsiEI CGRYCG 1 cut(s) 442
BsiHKAI GWGCWC 2 cut(s) 508, 2167
BsiHKCI CYCGRG 3 cut(s) 513, 1170, 2263
BsiSI CCGG 1 cut(s) 270
BslFI GGGAC 3 cut(s) 496, 768, 1019
BslI CCNNNNNNNGG 4 cut(s) 263, 1251, 1415, 2327
BsmAI GTCTC 4 cut(s) 336, 890, 1003, 1936
BsmFI GGGAC 3 cut(s) 496, 768, 1019
BsmI GAATGC 1 cut(s) 1645
BsnI GGCC 4 cut(s) 671, 953, 1007, 1615
Bso31I GGTCTC 1 cut(s) 890
BsoBI CYCGRG 3 cut(s) 513, 1170, 2263
Bsp119I TTCGAA 1 cut(s) 530
Bsp1286I GDGCHC 3 cut(s) 508, 771, 2167
Bsp1407I TGTACA 1 cut(s) 1674
BspACI CCGC 1 cut(s) 1801
BspANI GGCC 4 cut(s) 671, 953, 1007, 1615
BspCNI CTCAG 7 cut(s) 358, 444, 1531, 1841, 2125, 2494, 2500
BspFNI CGCG 1 cut(s) 521
BspLI GGNNCC 5 cut(s) 163, 268, 756, 1335, 2151
BspMAI CTGCAG 1 cut(s) 1941
BspOI GCTAGC 1 cut(s) 1952
BspPI GGATC 5 cut(s) 423, 483, 1328, 1341, 1480
BspQI GCTCTTC 3 cut(s) 816, 1376, 2739
BspT104I TTCGAA 1 cut(s) 530
BspT107I GGYRCC 2 cut(s) 161, 2149
BspTI CTTAAG 2 cut(s) 1847, 1985
BspTNI GGTCTC 1 cut(s) 890
BsrDI GCAATG 3 cut(s) 1044, 1359, 1918
BsrGI TGTACA 1 cut(s) 1674
BsrI ACTGG 8 cut(s) 218, 890, 941, 995, 1226, 1616, 1912, 2645
BssECI CCNNGG 4 cut(s) 24, 426, 1665, 2013
BssSI CACGAG 1 cut(s) 1767
BssT1I CCWWGG 1 cut(s) 24
Bst2BI CACGAG 1 cut(s) 1767
Bst2UI CCWGG 7 cut(s) 427, 501, 1074, 1713, 2014, 2322, 2782
Bst6I CTCTTC 6 cut(s) 802, 816, 1356, 1376, 2459, 2739
BstAFI CTTAAG 2 cut(s) 1847, 1985
BstAPI GCANNNNNTGC 1 cut(s) 1402
BstAUI TGTACA 1 cut(s) 1674
BstBAI YACGTR 1 cut(s) 2567
BstBI TTCGAA 1 cut(s) 530
BstC8I GCNNGC 1 cut(s) 1950
BstENI CCTNNNNNAGG 1 cut(s) 261
BstF5I GGATG 4 cut(s) 930, 1327, 1897, 2287
BstFNI CGCG 1 cut(s) 521
BstH2I RGCGCY 1 cut(s) 2191
BstHHI GCGC 2 cut(s) 2190, 2836
BstMAI GTCTC 4 cut(s) 336, 890, 1003, 1936
BstMCI CGRYCG 1 cut(s) 442
BstMWI GCNNNNNNNGC 5 cut(s) 1402, 1559, 1945, 2390, 2814
BstNI CCWGG 7 cut(s) 427, 501, 1074, 1713, 2014, 2322, 2782
BstNSI RCATGY 1 cut(s) 2672
BstSCI CCNGG 8 cut(s) 269, 425, 499, 1072, 1711, 2012, 2320, 2780
BstSFI CTRYAG 2 cut(s) 1428, 1937
BstUI CGCG 1 cut(s) 521
BstV1I GCAGC 2 cut(s) 1585, 2633
BstV2I GAAGAC 2 cut(s) 201, 1029
BstX2I RGATCY 2 cut(s) 415, 1333
BstYI RGATCY 2 cut(s) 415, 1333
BsuI GTATCC 1 cut(s) 2148
BsuRI GGCC 4 cut(s) 671, 953, 1007, 1615
BtgZI GCGATG 1 cut(s) 551
BtrI CACGTC 1 cut(s) 1496
BtsCI GGATG 4 cut(s) 930, 1327, 1897, 2287
BtsI GCAGTG 2 cut(s) 1064, 2238
BtsIMutI CAGTG 7 cut(s) 225, 282, 771, 1064, 1482, 1905, 2238
Cac8I GCNNGC 1 cut(s) 1950
CfoI GCGC 2 cut(s) 2190, 2836
Cfr13I GGNCC 3 cut(s) 267, 755, 1164
DraI TTTAAA 2 cut(s) 114, 1608
DraIII CACNNNGTG 1 cut(s) 1769
DrdI GACNNNNNNGTC 1 cut(s) 1031
DriI GACNNNNNGTC 1 cut(s) 2413
DseDI GACNNNNNNGTC 1 cut(s) 1031
EaeI YGGCCR 4 cut(s) 669, 951, 1005, 1613
Eam1104I CTCTTC 6 cut(s) 802, 816, 1356, 1376, 2459, 2739
Eam1105I GACNNNNNGTC 1 cut(s) 2413
EarI CTCTTC 6 cut(s) 802, 816, 1356, 1376, 2459, 2739
Ecl136II GAGCTC 1 cut(s) 2165
Eco130I CCWWGG 1 cut(s) 24
Eco24I GRGCYC 2 cut(s) 771, 2167
Eco31I GGTCTC 1 cut(s) 890
Eco32I GATATC 1 cut(s) 345
Eco47I GGWCC 3 cut(s) 267, 755, 1164
Eco53kI GAGCTC 1 cut(s) 2165
Eco57I CTGAAG 3 cut(s) 783, 1101, 2763
Eco88I CYCGRG 3 cut(s) 513, 1170, 2263
EcoICRI GAGCTC 1 cut(s) 2165
EcoNI CCTNNNNNAGG 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 755
EcoRI GAATTC 2 cut(s) 532, 808
EcoRII CCWGG 7 cut(s) 425, 499, 1072, 1711, 2012, 2320, 2780
EcoRV GATATC 1 cut(s) 345
EcoT14I CCWWGG 1 cut(s) 24
EcoT22I ATGCAT 3 cut(s) 1327, 1645, 2259
EcoT38I GRGCYC 2 cut(s) 771, 2167
ErhI CCWWGG 1 cut(s) 24
FalI AAGNNNNNCTT 6 cut(s) 1482, 1514, 1979, 2011, 2736, 2768
FaqI GGGAC 3 cut(s) 496, 768, 1019
FauNDI CATATG 5 cut(s) 174, 661, 1267, 2227, 2277
FblI GTMKAC 1 cut(s) 1133
Fnu4HI GCNGC 2 cut(s) 1599, 2622
FokI GGATG 4 cut(s) 917, 1334, 1904, 2294
FriOI GRGCYC 2 cut(s) 771, 2167
Fsp4HI GCNGC 2 cut(s) 1599, 2622
GlaI GCGC 2 cut(s) 2189, 2835
GluI GCNGC 2 cut(s) 1599, 2622
GsuI CTGGAG 2 cut(s) 510, 2304
HaeII RGCGCY 1 cut(s) 2191
HaeIII GGCC 4 cut(s) 671, 953, 1007, 1615
HapII CCGG 1 cut(s) 270
HhaI GCGC 2 cut(s) 2190, 2836
Hin6I GCGC 2 cut(s) 2188, 2834
HinP1I GCGC 2 cut(s) 2188, 2834
HincII GTYRAC 2 cut(s) 525, 2418
HindII GTYRAC 2 cut(s) 525, 2418
HpaII CCGG 1 cut(s) 270
HphI GGTGA 8 cut(s) 223, 257, 415, 903, 1727, 1755, 1991, 2254
Hpy166II GTNNAC 9 cut(s) 525, 1106, 1134, 1245, 1449, 1515, 1675, 1982, 2418
Hpy8I GTNNAC 9 cut(s) 525, 1106, 1134, 1245, 1449, 1515, 1675, 1982, 2418
HpyCH4IV ACGT 5 cut(s) 649, 1495, 1523, 2052, 2566
HpyF10VI GCNNNNNNNGC 5 cut(s) 1402, 1559, 1945, 2390, 2814
HpySE526I ACGT 5 cut(s) 649, 1495, 1523, 2052, 2566
HspAI GCGC 2 cut(s) 2188, 2834
KpnI GGTACC 1 cut(s) 2153
LguI GCTCTTC 3 cut(s) 816, 1376, 2739
LmnI GCTCC 4 cut(s) 383, 503, 928, 2323
Lsp1109I GCAGC 2 cut(s) 1585, 2633
LweI GCATC 7 cut(s) 574, 589, 1312, 1861, 2272, 2371, 2698
MaeII ACGT 5 cut(s) 649, 1495, 1523, 2052, 2566
MflI RGATCY 2 cut(s) 415, 1333
MhlI GDGCHC 3 cut(s) 508, 771, 2167
MlsI TGGCCA 2 cut(s) 671, 1615
MluNI TGGCCA 2 cut(s) 671, 1615
MlyI GAGTC 8 cut(s) 377, 525, 647, 685, 1139, 2254, 2281, 2792
MmeI TCCRAC 3 cut(s) 1645, 2064, 2762
Mox20I TGGCCA 2 cut(s) 671, 1615
Mph1103I ATGCAT 3 cut(s) 1327, 1645, 2259
MroXI GAANNNNTTC 1 cut(s) 1786
MscI TGGCCA 2 cut(s) 671, 1615
MseI TTAA 7 cut(s) 9, 113, 1607, 1755, 1848, 1986, 2309
MslI CAYNNNNRTG 2 cut(s) 744, 2280
Msp20I TGGCCA 2 cut(s) 671, 1615
MspCI CTTAAG 2 cut(s) 1847, 1985
MspI CCGG 1 cut(s) 270
MspR9I CCNGG 8 cut(s) 271, 427, 501, 1074, 1713, 2014, 2322, 2782
MssI GTTTAAAC 1 cut(s) 1608
Mva1269I GAATGC 1 cut(s) 1645
MvaI CCWGG 7 cut(s) 427, 501, 1074, 1713, 2014, 2322, 2782
MvnI CGCG 1 cut(s) 521
MwoI GCNNNNNNNGC 5 cut(s) 1402, 1559, 1945, 2390, 2814
NciI CCSGG 1 cut(s) 271
NdeI CATATG 5 cut(s) 174, 661, 1267, 2227, 2277
NheI GCTAGC 1 cut(s) 1948
NlaIV GGNNCC 5 cut(s) 163, 268, 756, 1335, 2151
NmeAIII GCCGAG 1 cut(s) 1690
NmuCI GTSAC 5 cut(s) 229, 909, 1761, 2609, 2684
NsiI ATGCAT 3 cut(s) 1327, 1645, 2259
NspI RCATGY 1 cut(s) 2672
NspV TTCGAA 1 cut(s) 530
PciSI GCTCTTC 3 cut(s) 816, 1376, 2739
PctI GAATGC 1 cut(s) 1645
PdmI GAANNNNTTC 1 cut(s) 1786
PfeI GAWTC 8 cut(s) 155, 736, 848, 1271, 1282, 1480, 2028, 2641
PfoI TCCNGGA 2 cut(s) 2320, 2780
PkrI GCNGC 2 cut(s) 1600, 2623
PleI GAGTC 8 cut(s) 376, 524, 646, 684, 1138, 2253, 2280, 2792
PmeI GTTTAAAC 1 cut(s) 1608
PpsI GAGTC 8 cut(s) 376, 524, 646, 684, 1138, 2253, 2280, 2792
Ppu21I YACGTR 1 cut(s) 2567
PpuMI RGGWCCY 1 cut(s) 755
PsiI TTATAA 1 cut(s) 2693
Psp124BI GAGCTC 1 cut(s) 2167
Psp5II RGGWCCY 1 cut(s) 755
Psp6I CCWGG 7 cut(s) 425, 499, 1072, 1711, 2012, 2320, 2780
PspGI CCWGG 7 cut(s) 425, 499, 1072, 1711, 2012, 2320, 2780
PspN4I GGNNCC 5 cut(s) 163, 268, 756, 1335, 2151
PspPI GGNCC 3 cut(s) 267, 755, 1164
PspPPI RGGWCCY 1 cut(s) 755
PstI CTGCAG 1 cut(s) 1941
PsuI RGATCY 2 cut(s) 415, 1333
RseI CAYNNNNRTG 2 cut(s) 744, 2280
SacI GAGCTC 1 cut(s) 2167
SapI GCTCTTC 3 cut(s) 816, 1376, 2739
SaqAI TTAA 7 cut(s) 9, 113, 1607, 1755, 1848, 1986, 2309
SatI GCNGC 2 cut(s) 1599, 2622
Sau96I GGNCC 3 cut(s) 267, 755, 1164
ScaI AGTACT 4 cut(s) 1117, 1991, 2306, 2334
SchI GAGTC 8 cut(s) 377, 525, 647, 685, 1139, 2254, 2281, 2792
ScrFI CCNGG 8 cut(s) 271, 427, 501, 1074, 1713, 2014, 2322, 2782
SduI GDGCHC 3 cut(s) 508, 771, 2167
SfaNI GCATC 7 cut(s) 574, 589, 1312, 1861, 2272, 2371, 2698
SfcI CTRYAG 2 cut(s) 1428, 1937
SfuI TTCGAA 1 cut(s) 530
SinI GGWCC 3 cut(s) 267, 755, 1164
SmiMI CAYNNNNRTG 2 cut(s) 744, 2280
SmlI CTYRAG 4 cut(s) 1847, 1985, 2526, 2585
SmoI CTYRAG 4 cut(s) 1847, 1985, 2526, 2585
SpeI ACTAGT 2 cut(s) 1112, 1118
SsiI CCGC 1 cut(s) 1801
SspI AATATT 1 cut(s) 2401
SstI GAGCTC 1 cut(s) 2167
StyD4I CCNGG 8 cut(s) 269, 425, 499, 1072, 1711, 2012, 2320, 2780
StyI CCWWGG 1 cut(s) 24
TaiI ACGT 5 cut(s) 652, 1498, 1526, 2055, 2569
TaqI TCGA 3 cut(s) 530, 2161, 2673
TaqII GACCGA 1 cut(s) 428
TatI WGTACW 7 cut(s) 1115, 1674, 1989, 2304, 2332, 2647, 2761
TfiI GAWTC 8 cut(s) 155, 736, 848, 1271, 1282, 1480, 2028, 2641
Tru1I TTAA 7 cut(s) 9, 113, 1607, 1755, 1848, 1986, 2309
Tru9I TTAA 7 cut(s) 9, 113, 1607, 1755, 1848, 1986, 2309
TscAI CASTG 7 cut(s) 225, 282, 778, 1071, 1482, 1912, 2238
TseFI GTSAC 5 cut(s) 229, 909, 1761, 2609, 2684
TseI GCWGC 2 cut(s) 1598, 2621
Tsp45I GTSAC 5 cut(s) 229, 909, 1761, 2609, 2684
TspGWI ACGGA 1 cut(s) 370
TspRI CASTG 7 cut(s) 225, 282, 778, 1071, 1482, 1912, 2238
Vha464I CTTAAG 2 cut(s) 1847, 1985
VpaK11BI GGWCC 3 cut(s) 267, 755, 1164
XagI CCTNNNNNAGG 1 cut(s) 261
XapI RAATTY 5 cut(s) 532, 552, 808, 2295, 2557
XbaI TCTAGA 1 cut(s) 761
XceI RCATGY 1 cut(s) 2672
XmiI GTMKAC 1 cut(s) 1133
XmnI GAANNNNTTC 1 cut(s) 1786
ZrmI AGTACT 4 cut(s) 1117, 1991, 2306, 2334
Zsp2I ATGCAT 3 cut(s) 1327, 1645, 2259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.