Rw1G028110

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000071
Physical Location & Seq
Reverse (-)
55635117 .. 55638357
3241 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G028110.1

Sequence Viewer

Length: 2319 bp
ATGGAAACTCAGAAGACCATGAAATTTTTGATGGGTCTCAATGAAAATTATGCCACGGTTCGAAGCAATATCGTGGGAATGGACCCTCTTCCAAGCGTGAATAAAGCATATTCCATGGCACTCCGACATGAGAAGAAAGCAGAGGTGTCAAGTGGAAAGGCATTAACTCAGCCGGAAGCTGCTGCCTTTGTTGTGAAGAAAGCCAACCGTGAGTTTGAAGTTGGAGAAGGTGAAACTAAATGTGAAAAGTGCAATAAAACCAATCATAGCACAAAGAATTGCAGGGCACACCTCAAGTGTACAAAGGCTGCTATGGAGGGAGGACAAGTACGTGTGAAAGGAAATCACGTTGTTTGCATGAATGATAAAAGAGAAGCTGCTCCTAGTTTCCCATTTTCACAGGAAGAGTGCCAGCAGCTCATGAGTCTCCTAAACAATAACAAAACTGCCACAGCAAATCAAGTTGGTAATGTTCCAGACTATGATGAACTCTCAGGTAAAGCGTTCACTCTCTCTCAAAATGGAAAAGAAACAATATGGATATTGGATAGTGGGGCTAGTGATCACATAGACCTACGATCGGGGAAGATGATTGGGATGGGAACTGAGAAGGAGGGACTCTACTGCCTCGACCCCGTGCAGAAAAGTTCATGCAATTCCATTCACAGCAAAACACACATCCTCTGGCATCAACGACTCGGTCACCCTTCAAGCAAATCTTCTTTCGAATTGATACATGTCGACATTTGGGGTGGATATCATGTTCCTTCAATTTCTGGTGCCAAATACTTTCTCACCATAGTTGATGATCACACTAGGAGCAATTGGGTGTATTTGATGAAACATAAGTCAGAAACACAAAATTTTCTCGTTAACTTCATTCACATGGTTGAAAATCAATTTGATAAGAGGGTTAAAGTTGTAGTTTATGATATTGCCAAACACAAAGTTCTTGTTTCTAGAGACGTTTTATTCTTTGAAGATGTCTTTCCATTCCAATCCCACACAAATTCGAATTCTCCATCTCCTCTGCCTCTTTTTCCGCAACCATTACAATTTTCATATCTTGATTCTGAACTTCCAATTTCGTCTCCACCAAAGCATGAAAACTCAATCCATGAAAATCCTCATCATGATCTTTCTTCCGCTGCAAATCACTCTTCTTCTCCTAATTCACATACTGAGCCTACCACCATGACTCGTTCACCACCATCACCAATACGTCGGTCCTCCAGACCTACCAAAACTCCATCATATTTGCAGGATTTCCATGTCGAGGCCTCCCTCCCCTCCCGACTCACACCGTCATCTTCAACGAACGTGGTTCTTCCCTCAGGTACTGCTCACCCTCTTTCTCATGTTTTATCTTATGATAGACTTTCTTCTCACCACCGAGTCTTCACCACTAAACTCACAATCCACCGAGAACCTACTTCTTTTTCCCAGGCCGTTCAGAGTCCACAATGGAGAGAGGCAATGCAAAAGGAGATTCAAGCACTCCAAGCAAACAATACTTGGAGTTTGGTGCCCTTACCATCTCATAAACGACCAATTGGTTGCAAATGGGTTTACAAGATCAAACTCAATCCAGATGGCACTATTCAGAGATACAAGGCACGCCTAGTTGCTAAGGGATACAGTCAGATTGAAGGGAGTTCTTCTTATCTTGCCGCTATGCAAGGTTGGCATTTACACCAATTGGACGTGAACAATGTGTTTCTTCATGGTGATCTATATGAAGAAGTCTACATGCATATACCTCCTGGGTTCAGACGAAAGGGGGAGACTAGGGTTTGCAAGCTACACAAGTCACTTTACGGGTTAAAACAAGCTTCAAGTCAATGGTTCATCAAGCTTTCAACAGCTCTCAAGTCTGCAGGGTTCCATCAATCTTGGTCTGATTATTCACTATTCGTCCGAACTCATCAGGGAAAATTCACAGCTTTATTGGTTTATGTTGACGATGTAATCTTGGCAGGAAACAGTTTGAAAGATATCATGGACACCAAACAATTTCTTTCCAGTCATTTCAAGCTAAAGGATATGGGGCAGGTAAAGTACTTTTTGGGAATAGAAATGGCCAGATCAAAGCAAGGCATTGCAATATGTCAGAGGAAGTATGCCCTAGAGATTCTTGAAGACACAGGTTTTCTTGGTGCCAAGTCCTCACGTTTCCCTGTTGATCAAAGCATATCACTCACACAAACAAATAGAGATTTGCTAGATGATCCATCACAATATCGCAGATTGGTAGGGCGGCTTATATACCTTACTATTACAAGGCCTAATCTTGTTTATGTTGTCCACTTACTAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2

Protein Analysis

772

Amino Acids

87.15

Weight (kDa)

9.27

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 503 - 727 2.3e-57 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2041
AccB1I GGYRCC 3 cut(s) 779, 1525, 2156
AccI GTMKAC 2 cut(s) 741, 1746
AciI CCGC 4 cut(s) 1043, 1146, 1671, 2257
AclWI GGATC 1 cut(s) 2222
AcoI YGGCCR 1 cut(s) 2079
AcsI RAATTY 5 cut(s) 23, 862, 1009, 1015, 1934
AfaI GTAC 4 cut(s) 301, 330, 1339, 2060
AfiI CCNNNNNNNGG 2 cut(s) 580, 1276
AflIII ACRYGT 2 cut(s) 331, 736
AjiI CACGTC 1 cut(s) 1705
AjnI CCWGG 2 cut(s) 1443, 1762
AloI GAACNNNNNNTCC 2 cut(s) 747, 779
AluBI AGCT 9 cut(s) 179, 377, 418, 1801, 1832, 1855, 1865, 1943, 2035
AluI AGCT 9 cut(s) 179, 377, 418, 1801, 1832, 1855, 1865, 1943, 2035
Alw26I GTCTC 5 cut(s) 41, 431, 957, 1095, 1778
AlwI GGATC 1 cut(s) 2222
AlwNI CAGNNNCTG 1 cut(s) 1340
AoxI GGCC 4 cut(s) 1278, 1446, 2079, 2282
ApeKI GCWGC 6 cut(s) 179, 182, 308, 377, 415, 1148
ApoI RAATTY 5 cut(s) 23, 862, 1009, 1015, 1934
AspS9I GGNCC 2 cut(s) 82, 1227
AsuHPI GGTGA 9 cut(s) 242, 695, 787, 1197, 1206, 1337, 1379, 1393, 1739
AsuII TTCGAA 3 cut(s) 61, 726, 1013
AvaII GGWCC 2 cut(s) 82, 1227
AxyI CCTNAGG 1 cut(s) 1333
BaeGI GKGCMC 2 cut(s) 289, 1530
BalI TGGCCA 1 cut(s) 2081
BanI GGYRCC 3 cut(s) 779, 1525, 2156
BbsI GAAGAC 3 cut(s) 20, 1390, 2145
BbvI GCAGC 6 cut(s) 166, 169, 295, 364, 427, 1135
BccI CCATC 9 cut(s) 25, 592, 1030, 1219, 1258, 1543, 1586, 1893, 2239
BceAI ACGGC 1 cut(s) 1433
BciT130I CCWGG 2 cut(s) 1445, 1764
BciVI GTATCC 1 cut(s) 1628
BclI TGATCA 3 cut(s) 562, 808, 2182
BcoDI GTCTC 5 cut(s) 41, 431, 957, 1095, 1778
BfaI CTAG 8 cut(s) 384, 558, 816, 960, 1622, 1788, 2126, 2222
BfmI CTRYAG 1 cut(s) 1875
BfuAI ACCTGC 1 cut(s) 2041
BfuI GTATCC 1 cut(s) 1628
BisI GCNGC 8 cut(s) 180, 183, 309, 378, 416, 1149, 1671, 2258
BlsI GCNGC 8 cut(s) 181, 184, 310, 379, 417, 1150, 1672, 2259
BmcAI AGTACT 1 cut(s) 2060
Bme1390I CCNGG 2 cut(s) 1445, 1764
Bme18I GGWCC 2 cut(s) 82, 1227
BmgBI CACGTC 1 cut(s) 1705
BmgT120I GGNCC 2 cut(s) 82, 1227
BmiI GGNNCC 5 cut(s) 84, 781, 1527, 1883, 2158
BmrFI CCNGG 2 cut(s) 1445, 1764
BmsI GCATC 1 cut(s) 697
BpiI GAAGAC 3 cut(s) 20, 1390, 2145
BpmI CTGGAG 1 cut(s) 1216
Bpu10I CCTNAGC 1 cut(s) 1629
Bpu14I TTCGAA 3 cut(s) 61, 726, 1013
BpuEI CTTGAG 2 cut(s) 278, 1853
BsaAI YACGTR 1 cut(s) 332
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 4 cut(s) 54, 114, 1443, 1763
BsaXI ACNNNNNCTCC 4 cut(s) 312, 342, 1231, 1261
Bsc4I CCNNNNNNNGG 2 cut(s) 580, 1276
Bse1I ACTGG 1 cut(s) 2022
Bse21I CCTNAGG 1 cut(s) 1333
Bse3DI GCAATG 2 cut(s) 1482, 2097
BseBI CCWGG 2 cut(s) 1445, 1764
BseDI CCNNGG 4 cut(s) 54, 114, 1443, 1763
BseGI GGATG 2 cut(s) 603, 678
BseLI CCNNNNNNNGG 2 cut(s) 580, 1276
BseMI GCAATG 2 cut(s) 1482, 2097
BseMII CTCAG 6 cut(s) 23, 182, 507, 597, 1173, 1347
BseNI ACTGG 1 cut(s) 2022
BseRI GAGGAG 1 cut(s) 1017
BseSI GKGCMC 2 cut(s) 289, 1530
BseXI GCAGC 6 cut(s) 166, 169, 295, 364, 427, 1135
BsgI GTGCAG 1 cut(s) 659
Bsh1285I CGRYCG 1 cut(s) 581
BshFI GGCC 4 cut(s) 1280, 1448, 2081, 2284
BshNI GGYRCC 3 cut(s) 779, 1525, 2156
BsiEI CGRYCG 1 cut(s) 581
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 1 cut(s) 630
BslI CCNNNNNNNGG 2 cut(s) 580, 1276
BsmAI GTCTC 5 cut(s) 41, 431, 957, 1095, 1778
BsmBI CGTCTC 2 cut(s) 957, 1095
BsmFI GGGAC 1 cut(s) 630
BsnI GGCC 4 cut(s) 1280, 1448, 2081, 2284
Bso31I GGTCTC 1 cut(s) 41
Bsp119I TTCGAA 3 cut(s) 61, 726, 1013
Bsp1286I GDGCHC 2 cut(s) 289, 1530
Bsp1407I TGTACA 1 cut(s) 299
Bsp143I GATC 9 cut(s) 562, 578, 808, 1135, 1575, 1729, 2084, 2182, 2227
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 4 cut(s) 1043, 1146, 1671, 2257
BspANI GGCC 4 cut(s) 1280, 1448, 2081, 2284
BspCNI CTCAG 6 cut(s) 22, 181, 506, 598, 1174, 1346
BspHI TCATGA 2 cut(s) 420, 1132
BspLI GGNNCC 5 cut(s) 84, 781, 1527, 1883, 2158
BspMAI CTGCAG 1 cut(s) 1879
BspMI ACCTGC 1 cut(s) 2041
BspPI GGATC 1 cut(s) 2222
BspT104I TTCGAA 3 cut(s) 61, 726, 1013
BspT107I GGYRCC 3 cut(s) 779, 1525, 2156
BspTNI GGTCTC 1 cut(s) 41
BsrDI GCAATG 2 cut(s) 1482, 2097
BsrGI TGTACA 1 cut(s) 299
BsrI ACTGG 1 cut(s) 2022
BssECI CCNNGG 4 cut(s) 54, 114, 1443, 1763
BssMI GATC 9 cut(s) 562, 578, 808, 1135, 1575, 1729, 2084, 2182, 2227
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 1445, 1764
Bst4CI ACNGT 5 cut(s) 58, 209, 1305, 1640, 1985
Bst6I CTCTTC 3 cut(s) 93, 399, 1165
BstAUI TGTACA 1 cut(s) 299
BstBAI YACGTR 1 cut(s) 332
BstBI TTCGAA 3 cut(s) 61, 726, 1013
BstC8I GCNNGC 3 cut(s) 413, 1618, 1799
BstDEI CTNAG 8 cut(s) 9, 168, 493, 606, 1182, 1333, 1629, 2312
BstDSI CCRYGG 2 cut(s) 54, 114
BstEII GGTNACC 1 cut(s) 701
BstF5I GGATG 2 cut(s) 603, 678
BstKTI GATC 9 cut(s) 565, 581, 811, 1138, 1578, 1732, 2087, 2185, 2230
BstMAI GTCTC 5 cut(s) 41, 431, 957, 1095, 1778
BstMBI GATC 9 cut(s) 562, 578, 808, 1135, 1575, 1729, 2084, 2182, 2227
BstMCI CGRYCG 1 cut(s) 581
BstMWI GCNNNNNNNGC 2 cut(s) 1502, 1684
BstNI CCWGG 2 cut(s) 1445, 1764
BstNSI RCATGY 2 cut(s) 740, 1753
BstPI GGTNACC 1 cut(s) 701
BstSCI CCNGG 2 cut(s) 1443, 1762
BstSFI CTRYAG 1 cut(s) 1875
BstSLI GKGCMC 2 cut(s) 289, 1530
BstV1I GCAGC 6 cut(s) 166, 169, 295, 364, 427, 1135
BstV2I GAAGAC 3 cut(s) 20, 1390, 2145
Bsu36I CCTNAGG 1 cut(s) 1333
BsuI GTATCC 1 cut(s) 1628
BsuRI GGCC 4 cut(s) 1280, 1448, 2081, 2284
BtgI CCRYGG 2 cut(s) 54, 114
BtrI CACGTC 1 cut(s) 1705
BtsCI GGATG 2 cut(s) 603, 678
BveI ACCTGC 1 cut(s) 2041
Cac8I GCNNGC 3 cut(s) 413, 1618, 1799
CaiI CAGNNNCTG 1 cut(s) 1340
CciI TCATGA 2 cut(s) 420, 1132
Cfr13I GGNCC 2 cut(s) 82, 1227
Csp6I GTAC 4 cut(s) 300, 329, 1338, 2059
CspCI CAANNNNNGTGG 2 cut(s) 1302, 1337
CviQI GTAC 4 cut(s) 300, 329, 1338, 2059
DdeI CTNAG 8 cut(s) 9, 168, 493, 606, 1182, 1333, 1629, 2312
DpnI GATC 9 cut(s) 564, 580, 810, 1137, 1577, 1731, 2086, 2184, 2229
DpnII GATC 9 cut(s) 562, 578, 808, 1135, 1575, 1729, 2084, 2182, 2227
EaeI YGGCCR 1 cut(s) 2079
Eam1104I CTCTTC 3 cut(s) 93, 399, 1165
EarI CTCTTC 3 cut(s) 93, 399, 1165
Eco130I CCWWGG 1 cut(s) 114
Eco147I AGGCCT 2 cut(s) 1280, 2284
Eco31I GGTCTC 1 cut(s) 41
Eco32I GATATC 2 cut(s) 758, 1996
Eco47I GGWCC 2 cut(s) 82, 1227
Eco81I CCTNAGG 1 cut(s) 1333
Eco91I GGTNACC 1 cut(s) 701
EcoO65I GGTNACC 1 cut(s) 701
EcoRI GAATTC 1 cut(s) 1015
EcoRII CCWGG 2 cut(s) 1443, 1762
EcoRV GATATC 2 cut(s) 758, 1996
EcoT14I CCWWGG 1 cut(s) 114
EcoT22I ATGCAT 1 cut(s) 1755
ErhI CCWWGG 1 cut(s) 114
Esp3I CGTCTC 2 cut(s) 957, 1095
FalI AAGNNNNNCTT 2 cut(s) 703, 735
FaqI GGGAC 1 cut(s) 630
FbaI TGATCA 3 cut(s) 562, 808, 2182
FblI GTMKAC 2 cut(s) 741, 1746
Fnu4HI GCNGC 8 cut(s) 180, 183, 309, 378, 416, 1149, 1671, 2258
FokI GGATG 2 cut(s) 610, 665
Fsp4HI GCNGC 8 cut(s) 180, 183, 309, 378, 416, 1149, 1671, 2258
FspBI CTAG 8 cut(s) 384, 558, 816, 960, 1622, 1788, 2126, 2222
GluI GCNGC 8 cut(s) 180, 183, 309, 378, 416, 1149, 1671, 2258
GsuI CTGGAG 1 cut(s) 1216
HaeIII GGCC 4 cut(s) 1280, 1448, 2081, 2284
HapII CCGG 1 cut(s) 173
HincII GTYRAC 3 cut(s) 742, 874, 1960
HindII GTYRAC 3 cut(s) 742, 874, 1960
HindIII AAGCTT 2 cut(s) 1830, 1853
HpaI GTTAAC 1 cut(s) 874
HpaII CCGG 1 cut(s) 173
HphI GGTGA 9 cut(s) 242, 695, 787, 1197, 1206, 1337, 1379, 1393, 1739
Hpy188III TCNNGA 9 cut(s) 421, 476, 960, 1067, 1133, 1233, 1293, 1589, 2135
Hpy99I CGWCG 1 cut(s) 1227
HpyAV CCTTC 5 cut(s) 221, 604, 717, 777, 1643
HpyCH4III ACNGT 5 cut(s) 58, 209, 1305, 1640, 1985
HpyCH4IV ACGT 7 cut(s) 331, 348, 966, 1222, 1320, 1704, 2170
HpyF10VI GCNNNNNNNGC 2 cut(s) 1502, 1684
HpyF3I CTNAG 8 cut(s) 9, 168, 493, 606, 1182, 1333, 1629, 2312
HpySE526I ACGT 7 cut(s) 331, 348, 966, 1222, 1320, 1704, 2170
Ksp22I TGATCA 3 cut(s) 562, 808, 2182
KspAI GTTAAC 1 cut(s) 874
Kzo9I GATC 9 cut(s) 562, 578, 808, 1135, 1575, 1729, 2084, 2182, 2227
LmnI GCTCC 2 cut(s) 385, 819
Lsp1109I GCAGC 6 cut(s) 166, 169, 295, 364, 427, 1135
LweI GCATC 1 cut(s) 697
MaeI CTAG 8 cut(s) 384, 558, 816, 960, 1622, 1788, 2126, 2222
MaeII ACGT 7 cut(s) 331, 348, 966, 1222, 1320, 1704, 2170
MaeIII GTNAC 2 cut(s) 701, 1809
MalI GATC 9 cut(s) 564, 580, 810, 1137, 1577, 1731, 2086, 2184, 2229
MboI GATC 9 cut(s) 562, 578, 808, 1135, 1575, 1729, 2084, 2182, 2227
MfeI CAATTG 3 cut(s) 823, 1551, 1697
MhlI GDGCHC 2 cut(s) 289, 1530
MlsI TGGCCA 1 cut(s) 2081
MluNI TGGCCA 1 cut(s) 2081
MlyI GAGTC 7 cut(s) 433, 612, 690, 1192, 1290, 1404, 1465
MmeI TCCRAC 2 cut(s) 148, 202
Mox20I TGGCCA 1 cut(s) 2081
Mph1103I ATGCAT 1 cut(s) 1755
MscI TGGCCA 1 cut(s) 2081
MseI TTAA 4 cut(s) 164, 873, 915, 1823
MslI CAYNNNNRTG 1 cut(s) 884
Msp20I TGGCCA 1 cut(s) 2081
MspA1I CMGCKG 1 cut(s) 1148
MspI CCGG 1 cut(s) 173
MspR9I CCNGG 2 cut(s) 1445, 1764
MunI CAATTG 3 cut(s) 823, 1551, 1697
MvaI CCWGG 2 cut(s) 1445, 1764
MwoI GCNNNNNNNGC 2 cut(s) 1502, 1684
NcoI CCATGG 1 cut(s) 114
NdeII GATC 9 cut(s) 562, 578, 808, 1135, 1575, 1729, 2084, 2182, 2227
NlaIV GGNNCC 5 cut(s) 84, 781, 1527, 1883, 2158
NmuCI GTSAC 2 cut(s) 701, 1809
NsiI ATGCAT 1 cut(s) 1755
NspI RCATGY 2 cut(s) 740, 1753
NspV TTCGAA 3 cut(s) 61, 726, 1013
PagI TCATGA 2 cut(s) 420, 1132
PceI AGGCCT 2 cut(s) 1280, 2284
PciI ACATGT 1 cut(s) 736
PfeI GAWTC 3 cut(s) 1070, 1489, 2131
PflFI GACNNNGTC 1 cut(s) 699
PkrI GCNGC 8 cut(s) 181, 184, 310, 379, 417, 1150, 1672, 2259
Ple19I CGATCG 1 cut(s) 581
PleI GAGTC 7 cut(s) 432, 612, 690, 1192, 1290, 1403, 1464
PpsI GAGTC 7 cut(s) 432, 612, 690, 1192, 1290, 1403, 1464
Ppu21I YACGTR 1 cut(s) 332
PscI ACATGT 1 cut(s) 736
Psp6I CCWGG 2 cut(s) 1443, 1762
PspEI GGTNACC 1 cut(s) 701
PspGI CCWGG 2 cut(s) 1443, 1762
PspN4I GGNNCC 5 cut(s) 84, 781, 1527, 1883, 2158
PspPI GGNCC 2 cut(s) 82, 1227
PstI CTGCAG 1 cut(s) 1879
PstNI CAGNNNCTG 1 cut(s) 1340
PsyI GACNNNGTC 1 cut(s) 699
PvuI CGATCG 1 cut(s) 581
RsaI GTAC 4 cut(s) 301, 330, 1339, 2060
RsaNI GTAC 4 cut(s) 300, 329, 1338, 2059
RseI CAYNNNNRTG 1 cut(s) 884
SalI GTCGAC 1 cut(s) 740
SaqAI TTAA 4 cut(s) 164, 873, 915, 1823
SatI GCNGC 8 cut(s) 180, 183, 309, 378, 416, 1149, 1671, 2258
Sau3AI GATC 9 cut(s) 562, 578, 808, 1135, 1575, 1729, 2084, 2182, 2227
Sau96I GGNCC 2 cut(s) 82, 1227
ScaI AGTACT 1 cut(s) 2060
SchI GAGTC 7 cut(s) 433, 612, 690, 1192, 1290, 1404, 1465
ScrFI CCNGG 2 cut(s) 1445, 1764
SduI GDGCHC 2 cut(s) 289, 1530
SfaNI GCATC 1 cut(s) 697
SfcI CTRYAG 1 cut(s) 1875
SfuI TTCGAA 3 cut(s) 61, 726, 1013
SinI GGWCC 2 cut(s) 82, 1227
SmiMI CAYNNNNRTG 1 cut(s) 884
SmlI CTYRAG 2 cut(s) 293, 1868
SmoI CTYRAG 2 cut(s) 293, 1868
SseBI AGGCCT 2 cut(s) 1280, 2284
SsiI CCGC 4 cut(s) 1043, 1146, 1671, 2257
SspMI CTAG 8 cut(s) 384, 558, 816, 960, 1622, 1788, 2126, 2222
StuI AGGCCT 2 cut(s) 1280, 2284
StyD4I CCNGG 2 cut(s) 1443, 1762
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 5 cut(s) 58, 209, 1305, 1640, 1985
TaiI ACGT 7 cut(s) 334, 351, 969, 1225, 1323, 1707, 2173
TaqI TCGA 6 cut(s) 61, 630, 726, 741, 1013, 1275
TaqII GACCGA 2 cut(s) 689, 1215
TatI WGTACW 2 cut(s) 299, 2058
TauI GCSGC 2 cut(s) 1673, 2260
TfiI GAWTC 3 cut(s) 1070, 1489, 2131
Tru1I TTAA 4 cut(s) 164, 873, 915, 1823
Tru9I TTAA 4 cut(s) 164, 873, 915, 1823
TseFI GTSAC 2 cut(s) 701, 1809
TseI GCWGC 6 cut(s) 179, 182, 308, 377, 415, 1148
Tsp45I GTSAC 2 cut(s) 701, 1809
Tth111I GACNNNGTC 1 cut(s) 699
VpaK11BI GGWCC 2 cut(s) 82, 1227
XapI RAATTY 5 cut(s) 23, 862, 1009, 1015, 1934
XbaI TCTAGA 1 cut(s) 959
XceI RCATGY 2 cut(s) 740, 1753
XmiI GTMKAC 2 cut(s) 741, 1746
XspI CTAG 8 cut(s) 384, 558, 816, 960, 1622, 1788, 2126, 2222
ZrmI AGTACT 1 cut(s) 2060
Zsp2I ATGCAT 1 cut(s) 1755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.