MD07G1013900.v1.1

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000071
Physical Location & Seq
Forward (+)
1214015 .. 1217386
3372 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1013900.v1.1.491

Sequence Viewer

Length: 2895 bp
ATGGCTCAATATAATCATAGTGTTGTTATGCATCTTGGTGGAACAAATAAATGGATAATTGATACTGGGGCCACTGATCATGTGACAAGTGATCCTAGAGTGTTTGATGAGTTATGTGATTATGTTCGTGATCCGTATATTACTAGTGCAAATGGAGCACTTTCCCCTGTGAAGGGTGAAGGCACTATCTCTTTGACTCCAACCTTATCACTGGTCCGTGCTTTACTTGTTCCTGATGTTAAGTGCAATCTTTTGTCGGTAGGAAAACTTCTTGATACTTTATATTGTTCTGCTCACTTCTATCCTACGTATTGTTATTTTCGAGATATTCAAACTCAGAAGATAATTGGTTATGGTAAAAGGATAGGGGGTTTGTACATCTTGACTATGGAGGATACTGTTGTTTCCGGTTCAAATAATCATCAAGTTTTAAGTGCTAAAGTGGATAACAGACATCAAATTTGGTTGTGGCATCGACGATTGGGACATCCATCATTCAGTTATATGAAACATCTATTTCCTTCTTTGTTTTGCACTTGTAGTGATTCAGAGTTCAAGTGTGAAACATGTGTCATGGCTAAGAGTCATCGTGCTTCCTTTCCTACACCGTTTGATGTGCTACAAAAACATGTTTCTCTTGTTTTTGTCTCAAAGCTTCATCCGAAAGTGTTTGGGTGTATTGCGTATGTGCATGTCTACTCTCATCAGCGGAGTAAACTTGATGCATGTGCTCTCCGGTGTGTCTTTATTGGGTATGCTAACAATAAAAAAGGCTATAAATGTTATCATCCTCCGACTCAAAAAACTTATATTACCATGGATGTCACCTTCCATGAGGAAGTTTCGTATTTTGTGAAGCCCTCTTCCGACTCTCCACTTCAGGGGGAGAGGAGGAGTGAAGTGCAGATTCGAAGAGATGATATGGATGATGTGTTACAGACAGAGTTGGGAACAGAACCTATTATTTTGCGTGATACTAATCAGTCGGCTACAGATGATGATCGGTTGCCTGTTGCGGATATATCTGATGAATTACCTTCTGATGATCGGTTGCCTGCTGCTGATATGTCTAACGAGTTGCCTGATGATGGTTCGTCCAGTGATGATTCTTCTAACTGTTTGGTACAAGATGGTGGCATACATGAGGTAAATTCTGACGATTCTTCTACTTATCAGTTGGCTCCACGAGCTAATCGTGGAAAACCTAAAGTACAATATGAGCCTGATATGCATGCCAAAGCCAAATATCCTATCAATAATTATGTGTCTTCTCATCGTTTGTCCCAACCATATGCATCTTACATATGTCAGCTATCTAGTGTATCAATTCCAACAAAATTGCAAGATGCTTTGTCTCACCTTAAGTGGGTTAATGCTATGAAAGTTGAGATGGAAGCTTTGGAAAAGAATTCTACTTGGGATTTGGTTCCGTTACCAAACGGGAAGAAAGCCGTTGGATGTAGATGGGTGTTTACTATTAAGCACAAAACAGATGGTTCTATTGATCGGTATAAAGCCAGATTAGTTGCTAAGGGTTATACTCAAACCTATGGGGTGGACTATCAGGAGACCTTTGCTCCTGTTGCCAAACTTAATACTGTGCGTGTTCTTCTGTCCTTGGTAGCAAACCAGGATTGGCCTCTGTTGCAGTTTGATGTTAAGAATGCTTTCCTTCATGGTGATCTTAAGGAGGAAGTGTATATGGATCTCCCACCTGGTATTGGAACATCTCCTGGAAAAGGTATTGTATGCAGGTTACGAAAGGCCTTGTATGGTTTGAAACAATCTCCTAGAGCATGGTTTGGGAGGTTTACAAGTTCAATGAAGAAGTTTGGGTATATCCAGAGTCATTCAGATCATACTTTGTTTCTAAAGCGACAAAATGGTAAGCTAACTGCATTGATTATTTATGTTGATGACATGATAGTGACTGGTGATGATCAAAAGGAGATACAACACCTTCAAAAGTACCTAGCTACTGAGTTTGAGATGAAAGAATTGGGTGAATTGAAGTATTTTCTTGGAATCGAGGTTGCACGATCCAAGCATGGTATTTTTCTATCTCAAAGGAAGTATGTTCTTGATTTGTTAGCTGAAACAGGTATGTTAGATTGCAAACCTGTTGATACTCCGATTGAGCAAAATCATCGTCTGGGCTTATTTCCAGATCAAGTTCCAACTCATAAGGAACGGTATCAGAGGCTTGTGGGGAGATTAATTTATTTGTCTCACACTCGCCCTGACATTGCTTATGCAGTTAGTGTGGTAAGTCAGTTTATGCACTCACCTAGTGAAGCTCATATGGATACAGTAACTCGTATTTTGAGGTACTTGAAGATGGCTCCTGGCAGAGGCCTGGTTTTCTCCAAGAATGATCATTTGAATGTCGAAGGGTATACAGATGCAGATTGGGCTGGTTCTATCACTGATCGGCGATCTACATCTGGATACTTTATGTTTGTGGGTGGTAATTTAGTTACTTGGAGAAGCAAGAAACAAAAAGTGGTGGCTAGGTCAAGTGCAAAAGCTGAGTTTCGTGGTATGTCTCATGGTGTATGTGAGTTGTTGTGGTTGGAAAAATTGTTGAGAGATCTTGGGTTTAAACACAAAGGTGCTATGAAACTTCATTGTGATAACAAGGCTGCTATTGAGATTGCTCATAATCCAGTGCAACATGATCGAACAAAACATGTGGAGATTGATCGACATTTCATTAAGGAAAAATTGGATGCTGGAATTATTATGTTTCCGTTTGTGAGATCTGAAGATCAACTGGCTGATGCTCTTACTAAGGCTGTGTCTAATAGTGCGTTTTCCGACTCGCTTGACAAGTTGGGCATCGTGACATCTTTGCACCAACTTGAGGGGGAGTGTTGCGAGTTATACATTAGTTAA

Protein Analysis

965

Amino Acids

108.95

Weight (kDa)

8.06

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pol_BBD PF22936 18 - 92 5.1e-12 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 124 - 195 3.2e-16 GAG-pre-integrase domain
SH3_retrovirus PF25597 226 - 288 2.7e-22 Retroviral polymerase SH3-like domain
RVT_2 PF07727 470 - 713 5.8e-72 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1743
AccI GTMKAC 2 cut(s) 696, 2396
AciI CCGC 2 cut(s) 709, 1016
AclWI GGATC 4 cut(s) 86, 125, 1713, 2034
AcsI RAATTY 3 cut(s) 459, 1150, 1408
AcuI CTGAAG 2 cut(s) 863, 2784
AdeI CACNNNGTG 1 cut(s) 2291
AfaI GTAC 5 cut(s) 377, 1125, 1212, 1970, 2330
AfiI CCNNNNNNNGG 9 cut(s) 172, 173, 881, 1088, 1366, 1721, 1739, 2351, 2863
AflII CTTAAG 2 cut(s) 1361, 1685
AflIII ACRYGT 3 cut(s) 566, 628, 2689
AgsI TTSAA 9 cut(s) 332, 414, 556, 1780, 1821, 1964, 2011, 2335, 2383
AhlI ACTAGT 1 cut(s) 143
AjnI CCWGG 5 cut(s) 1629, 1714, 1732, 2344, 2355
AleI CACNNNNGTG 1 cut(s) 2610
AluBI AGCT 9 cut(s) 655, 1190, 1312, 1397, 1891, 1976, 2093, 2297, 2528
AluI AGCT 9 cut(s) 655, 1190, 1312, 1397, 1891, 1976, 2093, 2297, 2528
Alw21I GWGCWC 2 cut(s) 160, 733
Alw26I GTCTC 5 cut(s) 652, 1359, 1562, 2232, 2550
AlwI GGATC 4 cut(s) 86, 125, 1713, 2034
AoxI GGCC 4 cut(s) 69, 1637, 1764, 2353
ApeKI GCWGC 2 cut(s) 1058, 2642
ApoI RAATTY 3 cut(s) 459, 1150, 1408
ArsI GACNNNNNNTTYG 4 cut(s) 444, 476, 2134, 2166
AseI ATTAAT 1 cut(s) 2216
Asp700I GAANNNNTTC 1 cut(s) 1667
AspS9I GGNCC 2 cut(s) 69, 214
AsuHPI GGTGA 7 cut(s) 188, 817, 1349, 1691, 1946, 2015, 2277
AsuII TTCGAA 1 cut(s) 910
AvaII GGWCC 1 cut(s) 214
BaeI ACNNNNGTAYC 2 cut(s) 387, 420
BarI GAAGNNNNNNTAC 2 cut(s) 252, 284
BauI CACGAG 1 cut(s) 1185
BbsI GAAGAC 1 cut(s) 1260
Bbv12I GWGCWC 2 cut(s) 160, 733
BbvI GCAGC 2 cut(s) 1045, 2629
BccI CCATC 7 cut(s) 499, 1082, 1124, 1384, 1458, 1487, 2332
BceAI ACGGC 1 cut(s) 1436
BcgI CGANNNNNNTGC 4 cut(s) 2129, 2163, 2660, 2694
BciT130I CCWGG 5 cut(s) 1631, 1716, 1734, 2346, 2357
BciVI GTATCC 3 cut(s) 388, 2299, 2441
BclI TGATCA 3 cut(s) 76, 1939, 2374
BcoDI GTCTC 5 cut(s) 652, 1359, 1562, 2232, 2550
BcuI ACTAGT 1 cut(s) 143
BfaI CTAG 7 cut(s) 96, 144, 1317, 1791, 1973, 2289, 2511
BfmI CTRYAG 1 cut(s) 990
BfrI CTTAAG 2 cut(s) 1361, 1685
BfuAI ACCTGC 1 cut(s) 1743
BfuI GTATCC 3 cut(s) 388, 2299, 2441
BglII AGATCT 2 cut(s) 2590, 2759
BisI GCNGC 2 cut(s) 1059, 2643
BlsI GCNGC 2 cut(s) 1060, 2644
Bme1390I CCNGG 5 cut(s) 1631, 1716, 1734, 2346, 2357
Bme18I GGWCC 1 cut(s) 214
BmgT120I GGNCC 2 cut(s) 69, 214
BmiI GGNNCC 4 cut(s) 70, 1182, 1428, 2343
BmrFI CCNGG 5 cut(s) 1631, 1716, 1734, 2346, 2357
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 9 cut(s) 40, 481, 712, 1304, 1336, 2392, 2719, 2770, 2847
BmuI ACTGGG 1 cut(s) 75
BpiI GAAGAC 1 cut(s) 1260
Bpu10I CCTNAGC 1 cut(s) 1530
Bpu14I TTCGAA 1 cut(s) 910
BpuEI CTTGAG 1 cut(s) 2882
BsaAI YACGTR 1 cut(s) 309
BsaBI GATNNNNATC 3 cut(s) 978, 999, 2440
BsaI GGTCTC 1 cut(s) 1562
BsaJI CCNNGG 2 cut(s) 816, 1617
BsaWI WCCGGW 2 cut(s) 407, 735
BsaXI ACNNNNNCTCC 4 cut(s) 1561, 1591, 1682, 1712
Bsc4I CCNNNNNNNGG 9 cut(s) 172, 173, 881, 1088, 1366, 1721, 1739, 2351, 2863
Bse1I ACTGG 6 cut(s) 70, 216, 1098, 1936, 2666, 2778
Bse3DI GCAATG 1 cut(s) 2244
Bse8I GATNNNNATC 3 cut(s) 978, 999, 2440
BseBI CCWGG 5 cut(s) 1631, 1716, 1734, 2346, 2357
BseDI CCNNGG 2 cut(s) 816, 1617
BseGI GGATG 7 cut(s) 487, 658, 787, 826, 931, 1463, 2734
BseJI GATNNNNATC 3 cut(s) 978, 999, 2440
BseLI CCNNNNNNNGG 9 cut(s) 172, 173, 881, 1088, 1366, 1721, 1739, 2351, 2863
BseMI GCAATG 1 cut(s) 2244
BseMII CTCAG 3 cut(s) 350, 1971, 2520
BseNI ACTGG 6 cut(s) 70, 216, 1098, 1936, 2666, 2778
BseRI GAGGAG 2 cut(s) 904, 907
BseXI GCAGC 2 cut(s) 1045, 2629
BsgI GTGCAG 1 cut(s) 923
BshFI GGCC 4 cut(s) 71, 1639, 1766, 2355
BsiHKAI GWGCWC 2 cut(s) 160, 733
BsiSI CCGG 2 cut(s) 408, 736
BslFI GGGAC 2 cut(s) 498, 1267
BslI CCNNNNNNNGG 9 cut(s) 172, 173, 881, 1088, 1366, 1721, 1739, 2351, 2863
BsmAI GTCTC 5 cut(s) 652, 1359, 1562, 2232, 2550
BsmFI GGGAC 2 cut(s) 498, 1267
BsmI GAATGC 1 cut(s) 1669
BsnI GGCC 4 cut(s) 71, 1639, 1766, 2355
Bso31I GGTCTC 1 cut(s) 1562
Bsp119I TTCGAA 1 cut(s) 910
Bsp1286I GDGCHC 2 cut(s) 160, 733
Bsp1407I TGTACA 1 cut(s) 375
Bsp19I CCATGG 1 cut(s) 816
BspACI CCGC 2 cut(s) 709, 1016
BspANI GGCC 4 cut(s) 71, 1639, 1766, 2355
BspCNI CTCAG 3 cut(s) 349, 1972, 2521
BspLI GGNNCC 4 cut(s) 70, 1182, 1428, 2343
BspMI ACCTGC 1 cut(s) 1743
BspPI GGATC 4 cut(s) 86, 125, 1713, 2034
BspT104I TTCGAA 1 cut(s) 910
BspTI CTTAAG 2 cut(s) 1361, 1685
BspTNI GGTCTC 1 cut(s) 1562
BsrDI GCAATG 1 cut(s) 2244
BsrGI TGTACA 1 cut(s) 375
BsrI ACTGG 6 cut(s) 70, 216, 1098, 1936, 2666, 2778
BssECI CCNNGG 2 cut(s) 816, 1617
BssNAI GTATAC 1 cut(s) 2397
BssSI CACGAG 1 cut(s) 1185
BssT1I CCWWGG 2 cut(s) 816, 1617
Bst1107I GTATAC 1 cut(s) 2397
Bst2BI CACGAG 1 cut(s) 1185
Bst2UI CCWGG 5 cut(s) 1631, 1716, 1734, 2346, 2357
Bst4CI ACNGT 6 cut(s) 400, 609, 1118, 1600, 2193, 2311
Bst6I CTCTTC 2 cut(s) 868, 907
BstAFI CTTAAG 2 cut(s) 1361, 1685
BstAUI TGTACA 1 cut(s) 375
BstBAI YACGTR 1 cut(s) 309
BstBI TTCGAA 1 cut(s) 910
BstC8I GCNNGC 2 cut(s) 1056, 1233
BstDEI CTNAG 6 cut(s) 336, 579, 1530, 1980, 2529, 2790
BstDSI CCRYGG 1 cut(s) 816
BstENI CCTNNNNNAGG 2 cut(s) 1737, 2349
BstF5I GGATG 7 cut(s) 487, 658, 787, 826, 931, 1463, 2734
BstMAI GTCTC 5 cut(s) 652, 1359, 1562, 2232, 2550
BstMWI GCNNNNNNNGC 6 cut(s) 155, 1187, 1228, 1583, 1645, 2411
BstNI CCWGG 5 cut(s) 1631, 1716, 1734, 2346, 2357
BstNSI RCATGY 6 cut(s) 570, 632, 695, 729, 1235, 2693
BstSCI CCNGG 5 cut(s) 1629, 1714, 1732, 2344, 2355
BstSFI CTRYAG 1 cut(s) 990
BstSNI TACGTA 1 cut(s) 309
BstV1I GCAGC 2 cut(s) 1045, 2629
BstV2I GAAGAC 1 cut(s) 1260
BstX2I RGATCY 3 cut(s) 1705, 2590, 2759
BstYI RGATCY 3 cut(s) 1705, 2590, 2759
BstZ17I GTATAC 1 cut(s) 2397
BsuI GTATCC 3 cut(s) 388, 2299, 2441
BsuRI GGCC 4 cut(s) 71, 1639, 1766, 2355
BtgI CCRYGG 1 cut(s) 816
BtsCI GGATG 7 cut(s) 487, 658, 787, 826, 931, 1463, 2734
BtsIMutI CAGTG 5 cut(s) 72, 209, 1105, 2424, 2673
BveI ACCTGC 1 cut(s) 1743
Cac8I GCNNGC 2 cut(s) 1056, 1233
Cfr13I GGNCC 2 cut(s) 69, 214
CsiI ACCWGGT 1 cut(s) 1714
Csp6I GTAC 5 cut(s) 376, 1124, 1211, 1969, 2329
CspCI CAANNNNNGTGG 2 cut(s) 2673, 2708
CviQI GTAC 5 cut(s) 376, 1124, 1211, 1969, 2329
DdeI CTNAG 6 cut(s) 336, 579, 1530, 1980, 2529, 2790
DraI TTTAAA 1 cut(s) 2602
DraIII CACNNNGTG 1 cut(s) 2291
Eam1104I CTCTTC 2 cut(s) 868, 907
EarI CTCTTC 2 cut(s) 868, 907
Eco105I TACGTA 1 cut(s) 309
Eco130I CCWWGG 2 cut(s) 816, 1617
Eco147I AGGCCT 2 cut(s) 1766, 2355
Eco31I GGTCTC 1 cut(s) 1562
Eco47I GGWCC 1 cut(s) 214
Eco57I CTGAAG 2 cut(s) 863, 2784
EcoNI CCTNNNNNAGG 2 cut(s) 1737, 2349
EcoRI GAATTC 1 cut(s) 1408
EcoRII CCWGG 5 cut(s) 1629, 1714, 1732, 2344, 2355
EcoT14I CCWWGG 2 cut(s) 816, 1617
EcoT22I ATGCAT 4 cut(s) 33, 727, 1233, 1297
ErhI CCWWGG 2 cut(s) 816, 1617
FaqI GGGAC 2 cut(s) 498, 1267
FauNDI CATATG 3 cut(s) 1291, 1304, 2301
FbaI TGATCA 3 cut(s) 76, 1939, 2374
FblI GTMKAC 2 cut(s) 696, 2396
Fnu4HI GCNGC 2 cut(s) 1059, 2643
FokI GGATG 7 cut(s) 474, 645, 774, 833, 938, 1470, 2741
Fsp4HI GCNGC 2 cut(s) 1059, 2643
FspBI CTAG 7 cut(s) 96, 144, 1317, 1791, 1973, 2289, 2511
GluI GCNGC 2 cut(s) 1059, 2643
HaeIII GGCC 4 cut(s) 71, 1639, 1766, 2355
HapII CCGG 2 cut(s) 408, 736
HindIII AAGCTT 2 cut(s) 653, 1395
HpaII CCGG 2 cut(s) 408, 736
HphI GGTGA 7 cut(s) 188, 817, 1349, 1691, 1946, 2015, 2277
Hpy166II GTNNAC 6 cut(s) 697, 716, 1473, 1558, 1812, 2397
Hpy8I GTNNAC 6 cut(s) 697, 716, 1473, 1558, 1812, 2397
Hpy99I CGWCG 1 cut(s) 480
HpyAV CCTTC 8 cut(s) 166, 173, 531, 838, 1047, 1682, 1970, 2384
HpyCH4III ACNGT 6 cut(s) 400, 609, 1118, 1600, 2193, 2311
HpyCH4IV ACGT 1 cut(s) 308
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 1187, 1228, 1583, 1645, 2411
HpyF3I CTNAG 6 cut(s) 336, 579, 1530, 1980, 2529, 2790
HpySE526I ACGT 1 cut(s) 308
Ksp22I TGATCA 3 cut(s) 76, 1939, 2374
LmnI GCTCC 4 cut(s) 155, 1186, 1582, 2347
Lsp1109I GCAGC 2 cut(s) 1045, 2629
LweI GCATC 9 cut(s) 40, 481, 712, 1304, 1336, 2392, 2719, 2770, 2847
MabI ACCWGGT 1 cut(s) 1714
MaeI CTAG 7 cut(s) 96, 144, 1317, 1791, 1973, 2289, 2511
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 9 cut(s) 82, 823, 933, 1431, 1755, 1927, 2311, 2476, 2842
MflI RGATCY 3 cut(s) 1705, 2590, 2759
MhlI GDGCHC 2 cut(s) 160, 733
MlyI GAGTC 6 cut(s) 190, 592, 790, 863, 1855, 2813
MmeI TCCRAC 8 cut(s) 224, 818, 891, 1355, 1435, 2201, 2553, 2841
Mph1103I ATGCAT 4 cut(s) 33, 727, 1233, 1297
MroXI GAANNNNTTC 1 cut(s) 1667
MslI CAYNNNNRTG 4 cut(s) 36, 1925, 2382, 2610
MspA1I CMGCKG 1 cut(s) 709
MspCI CTTAAG 2 cut(s) 1361, 1685
MspI CCGG 2 cut(s) 408, 736
MspR9I CCNGG 5 cut(s) 1631, 1716, 1734, 2346, 2357
MssI GTTTAAAC 1 cut(s) 2602
Mva1269I GAATGC 1 cut(s) 1669
MvaI CCWGG 5 cut(s) 1631, 1716, 1734, 2346, 2357
MwoI GCNNNNNNNGC 6 cut(s) 155, 1187, 1228, 1583, 1645, 2411
NcoI CCATGG 1 cut(s) 816
NdeI CATATG 3 cut(s) 1291, 1304, 2301
NlaIV GGNNCC 4 cut(s) 70, 1182, 1428, 2343
NmuCI GTSAC 4 cut(s) 82, 823, 1927, 2842
NsiI ATGCAT 4 cut(s) 33, 727, 1233, 1297
NspI RCATGY 6 cut(s) 570, 632, 695, 729, 1235, 2693
NspV TTCGAA 1 cut(s) 910
OliI CACNNNNGTG 1 cut(s) 2610
PaeI GCATGC 1 cut(s) 1235
PceI AGGCCT 2 cut(s) 1766, 2355
PciI ACATGT 3 cut(s) 566, 628, 2689
PcsI WCGNNNNNNNCGW 1 cut(s) 1192
PctI GAATGC 1 cut(s) 1669
PdmI GAANNNNTTC 1 cut(s) 1667
PfeI GAWTC 5 cut(s) 545, 907, 1106, 1160, 2025
PfoI TCCNGGA 1 cut(s) 1732
PkrI GCNGC 2 cut(s) 1060, 2644
PleI GAGTC 6 cut(s) 190, 591, 790, 863, 1854, 2813
PmeI GTTTAAAC 1 cut(s) 2602
PpsI GAGTC 6 cut(s) 190, 591, 790, 863, 1854, 2813
Ppu21I YACGTR 1 cut(s) 309
PscI ACATGT 3 cut(s) 566, 628, 2689
PshBI ATTAAT 1 cut(s) 2216
Psp6I CCWGG 5 cut(s) 1629, 1714, 1732, 2344, 2355
PspGI CCWGG 5 cut(s) 1629, 1714, 1732, 2344, 2355
PspN4I GGNNCC 4 cut(s) 70, 1182, 1428, 2343
PspPI GGNCC 2 cut(s) 69, 214
PsuI RGATCY 3 cut(s) 1705, 2590, 2759
RsaI GTAC 5 cut(s) 377, 1125, 1212, 1970, 2330
RsaNI GTAC 5 cut(s) 376, 1124, 1211, 1969, 2329
RseI CAYNNNNRTG 4 cut(s) 36, 1925, 2382, 2610
SatI GCNGC 2 cut(s) 1059, 2643
Sau96I GGNCC 2 cut(s) 69, 214
SchI GAGTC 6 cut(s) 190, 592, 790, 863, 1855, 2813
ScrFI CCNGG 5 cut(s) 1631, 1716, 1734, 2346, 2357
SduI GDGCHC 2 cut(s) 160, 733
SexAI ACCWGGT 1 cut(s) 1714
SfaNI GCATC 9 cut(s) 40, 481, 712, 1304, 1336, 2392, 2719, 2770, 2847
SfcI CTRYAG 1 cut(s) 990
SfuI TTCGAA 1 cut(s) 910
SinI GGWCC 1 cut(s) 214
SmiMI CAYNNNNRTG 4 cut(s) 36, 1925, 2382, 2610
SmlI CTYRAG 3 cut(s) 1361, 1685, 2861
SmoI CTYRAG 3 cut(s) 1361, 1685, 2861
SnaBI TACGTA 1 cut(s) 309
SpeI ACTAGT 1 cut(s) 143
SphI GCATGC 1 cut(s) 1235
SseBI AGGCCT 2 cut(s) 1766, 2355
SsiI CCGC 2 cut(s) 709, 1016
SspMI CTAG 7 cut(s) 96, 144, 1317, 1791, 1973, 2289, 2511
StuI AGGCCT 2 cut(s) 1766, 2355
StyD4I CCNGG 5 cut(s) 1629, 1714, 1732, 2344, 2355
StyI CCWWGG 2 cut(s) 816, 1617
TaaI ACNGT 6 cut(s) 400, 609, 1118, 1600, 2193, 2311
TaiI ACGT 1 cut(s) 311
TaqI TCGA 7 cut(s) 322, 475, 910, 2028, 2388, 2680, 2704
TatI WGTACW 2 cut(s) 375, 1210
TfiI GAWTC 5 cut(s) 545, 907, 1106, 1160, 2025
TscAI CASTG 5 cut(s) 79, 216, 1105, 2431, 2673
TseFI GTSAC 4 cut(s) 82, 823, 1927, 2842
TseI GCWGC 2 cut(s) 1058, 2642
Tsp45I GTSAC 4 cut(s) 82, 823, 1927, 2842
TspGWI ACGGA 4 cut(s) 123, 206, 1419, 2739
TspRI CASTG 5 cut(s) 79, 216, 1105, 2431, 2673
Vha464I CTTAAG 2 cut(s) 1361, 1685
VpaK11BI GGWCC 1 cut(s) 214
VspI ATTAAT 1 cut(s) 2216
XagI CCTNNNNNAGG 2 cut(s) 1737, 2349
XapI RAATTY 3 cut(s) 459, 1150, 1408
XceI RCATGY 6 cut(s) 570, 632, 695, 729, 1235, 2693
XmiI GTMKAC 2 cut(s) 696, 2396
XmnI GAANNNNTTC 1 cut(s) 1667
XspI CTAG 7 cut(s) 96, 144, 1317, 1791, 1973, 2289, 2511
Zsp2I ATGCAT 4 cut(s) 33, 727, 1233, 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.