MD01G1128200.v1.1

Reverse transcriptase (RNA-dependent DNA polymerase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000071
Physical Location & Seq
Forward (+)
23975556 .. 23984361
8806 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1128200.v1.1.491

Sequence Viewer

Length: 2409 bp
ATGGCAGGAGCAGAAGGCAACGCAATTGGGATTGATCTGGGGACAACGTACTCGTGTGTCGCAATGTGGGAGAACGACCATGTGGAAATCATAGTGAATGATCAGGGTAACAGAACCACTCCATCTTGTGTTGCCTTCACAGAGACTGAGCGATTGGTTGGTGATTCAGCCATCAACCAAGTCATCAGGAACCCGACAAACTCCATTTTCGTTATCCCTGCTTACTTCAATGATTCACAACGTCAGGCTACAAAAAATGCTTGCAGCATTGCTGGCCTAAATGTGATGCGTATCATCAACGAGCCAACTGCTGCAGCGATTGCTTATGGCATTGACAGGAAGGCTGGTTGGTATAGCAAGAGAAATGTGATGATATTTGATTTAGGTGGTGGTACTTTAGATGTTTCACTACTTACCATAGGTGATGGTTTGTTTCAAGTGAAGGCCACGGCTGGAGACACTCACCTTGGAGGAGAAGACTTAGATAACAAAATGGTGGATTTTTGTGTTGAGCAATTCAAGAAGAAGCATAATGTGGACGTGAGTGGGAACTCTAGAGCACTCAGGAGGTTAAGAAACGCATGTGAGAAAGCAAAGAGCAGGCTTTCATTTACTTCAGCCACTGACATTGAAATCGACTGTATGTATGAGGGTACTGATTTCTATACAACTATCACCCGTGCCAAATTTGAGCAACTGAATATATTGGCAAATCGATCAGGCCATGTATATTGGATTTGCAGCAGTGTGATCTTGTTGCTGCTGTGTGTGTGTGTAGTTGTTTGCTGCTTGTCTGCTACCATACAAATACTTGAGGCTGCTACGCTGCCAAGCACCGAAACAGTTGATGCTTCTCGGCAACAGTCTGTCGAGTATGATGCAGAGTATCGGCAGGAGTCTGCTGAACCCGAACCCGCTGCTGCAATGGATGAAGAAATGATGGTACTGCATAAGAATGATACGTGGGAGGTAACGGAGCTACCGAAAGGAAAAAAACCGGTCGGGTGTAGATGGGTCTTTACAATTAAATACAAGGCAGACGGATCAGTGGACATGTATAAAGCAAGGTTAGTAGCAAAGAGTTATACACAAACTTATGGTGTTGATTATCAGGAAACTTTTTCTCCAGTAGCGAAGATGAATACAGTACGAGTCCTTATTTCTTTAGCTGCAAATTTGAATTGGCCACTGAAACAGTTTGATGTGAAAAATGCTTTTCTTCATGGGTACCTGAAAGAAGAAGTATATATGGATTTTCCTCCGGGGTATAATGCCAGAAGGAAAACCGGAGTATGTAGGTTGCAAAAGTCACTTTACGGGCTTAAGCAATCACCTAATGCATGGTTTGGTAGATTCACTCAAGTTATGAGGAGGATTGGGTATTATCAAAGTCACTCTGATCACACGTTATATGTGAAACAGAGAAGTGGTAAAGTGATGGCCTTGATTATTTATGTGGATGACATGATAATAACAGGTGATGATTCGGATGAGATGATGAAATTAGAAAAAAGTCTTGCCGTTGAATTTGAGATGAAGAATTTGGGAGATTTGAAATATTTCCTTGGAGTGGAAGTCACCCGGTCATCTAAAGGTGATGTTACTGACAGACGGTCTACATATGGGTATTTCACATTTGTTGGAGGTAATTTAGTTACGTGGTGGAGTAAGAAGCAGAATGTGGTATCACGGTTCTCTGCCGAAGCCGAGTATAGGGGTATGGCACATGGTGTGTGTGAGATCCTTTGGCTACAAAAGTTGCTTTGGGGTCTTGGTTTTACACAGAAAGAGGCGATGAAATTGTATTGTGACAATAAGTCTGCTAGAGAAATAGCTGAGAATCTGATACAGCATGACAGGACCAAGCATGTTGAGGTTGATAGACATTTTATCAAAGAAAAGCTGGAAAAGAAGATTGTGTCAATACCGTTCGTGAACTCAAAGGAACAACTTGCGGATATCCTTACTCATGATTGTCTGGAGCCAGTAGAGAAGTGTTTGAAGGATGCCAATATGGAAGTGAGCAGTGTCCATGATGTTGTTCTTGCCGGTGGATCTTCTAGAATTCCCAAAGTGCAGCAACTATTGCAGGATATGTTCAAGGTGAAGCAGTTGTGCAAGAGTATCAATCCGGATGAGGCAATTGCGTATGGTGCCGCTGCTCAAGCTGCAGTCTTGAGTGGGAATGTGAATGGGAAGCTTCATGGCTTTTCTCTCTTAGATGTCACACCTCTGTCACTTGGGGTCAAGGAAGAAAATATCGGTAATATCGGAAATATCGGTAGTTCGAAAACACGGAAATATCGGGATAATATCGATATCGATAAAAATTACATGGAAACCACGGAAATTGGTATGGAAGATGTGGAAAGGGGAAGAGGGGGCAGAGAGAAAATTGGATGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

803

Amino Acids

89.82

Weight (kDa)

6.47

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 8 - 70 4.5e-24 Hsp70 protein
MreB_Mbl PF06723 67 - 181 6.2e-09 MreB/Mbl protein
HSP70 PF00012 71 - 234 8e-70 Hsp70 protein
RVT_2 PF07727 319 - 531 7.4e-65 Reverse transcriptase (RNA-dependent DNA polymerase)
HSP70 PF00012 649 - 751 1.2e-33 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1227
AccB1I GGYRCC 2 cut(s) 1227, 2153
AccI GTMKAC 1 cut(s) 1616
AccIII TCCGGA 1 cut(s) 2131
AciI CCGC 3 cut(s) 915, 1955, 2157
AclWI GGATC 3 cut(s) 1051, 1735, 2062
AcoI YGGCCR 1 cut(s) 1184
AcsI RAATTY 5 cut(s) 686, 1174, 1526, 1540, 2064
AcuI CTGAAG 1 cut(s) 600
AdeI CACNNNGTG 1 cut(s) 1730
AfaI GTAC 6 cut(s) 50, 394, 655, 945, 1149, 1229
AfiI CCNNNNNNNGG 2 cut(s) 1570, 1713
AflII CTTAAG 1 cut(s) 1322
AflIII ACRYGT 2 cut(s) 1053, 1404
AgeI ACCGGT 1 cut(s) 997
AgsI TTSAA 9 cut(s) 229, 437, 520, 632, 1180, 1526, 1555, 2002, 2101
AhdI GACNNNNNGTC 2 cut(s) 1612, 1816
AjiI CACGTC 1 cut(s) 541
AluBI AGCT 6 cut(s) 979, 1169, 1835, 1903, 2168, 2200
AluI AGCT 6 cut(s) 979, 1169, 1835, 1903, 2168, 2200
Alw21I GWGCWC 1 cut(s) 562
Alw26I GTCTC 2 cut(s) 137, 450
AlwI GGATC 3 cut(s) 1051, 1735, 2062
AlwNI CAGNNNCTG 2 cut(s) 146, 623
Aor13HI TCCGGA 1 cut(s) 2131
AoxI GGCC 5 cut(s) 274, 444, 721, 1184, 1440
ApoI RAATTY 5 cut(s) 686, 1174, 1526, 1540, 2064
AsiGI ACCGGT 1 cut(s) 997
Asp700I GAANNNNTTC 1 cut(s) 1559
Asp718I GGTACC 1 cut(s) 1227
AspS9I GGNCC 1 cut(s) 1860
AsuC2I CCSGG 2 cut(s) 1263, 1582
AsuHPI GGTGA 9 cut(s) 173, 434, 455, 667, 1323, 1490, 1570, 1607, 2116
AsuII TTCGAA 1 cut(s) 2288
AvaII GGWCC 1 cut(s) 1860
BalI TGGCCA 1 cut(s) 1186
BanI GGYRCC 2 cut(s) 1227, 2153
BauI CACGAG 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 483
Bbv12I GWGCWC 1 cut(s) 562
BccI CCATC 6 cut(s) 130, 179, 419, 934, 1005, 1432
BceAI ACGGC 2 cut(s) 465, 1505
BcgI CGANNNNNNTGC 6 cut(s) 290, 324, 860, 894, 899, 933
BclI TGATCA 2 cut(s) 100, 1399
BcnI CCSGG 2 cut(s) 1263, 1582
BcoDI GTCTC 2 cut(s) 137, 450
BfaI CTAG 4 cut(s) 555, 1824, 2061, 2407
BfmI CTRYAG 2 cut(s) 312, 2169
BfrI CTTAAG 1 cut(s) 1322
Bme1390I CCNGG 2 cut(s) 1263, 1582
Bme18I GGWCC 1 cut(s) 1860
BmeRI GACNNNNNGTC 2 cut(s) 1612, 1816
BmgBI CACGTC 1 cut(s) 541
BmgT120I GGNCC 1 cut(s) 1860
BmiI GGNNCC 4 cut(s) 191, 1229, 1983, 2155
BmrFI CCNGG 2 cut(s) 1263, 1582
BmsI GCATC 5 cut(s) 276, 838, 868, 1996, 2390
BpiI GAAGAC 1 cut(s) 483
BpmI CTGGAG 3 cut(s) 474, 1110, 2000
Bpu14I TTCGAA 1 cut(s) 2288
BpuEI CTTGAG 4 cut(s) 833, 1344, 2148, 2197
BpuMI CCSGG 2 cut(s) 1263, 1582
Bsa29I ATCGAT 3 cut(s) 715, 2316, 2322
BsaAI YACGTR 2 cut(s) 963, 1659
BsaBI GATNNNNATC 1 cut(s) 290
BsaJI CCNNGG 5 cut(s) 447, 466, 1262, 1564, 2343
BsaWI WCCGGW 3 cut(s) 997, 1286, 2131
BsaXI ACNNNNNCTCC 4 cut(s) 1108, 1138, 1560, 1590
Bsc4I CCNNNNNNNGG 2 cut(s) 1570, 1713
Bse118I RCCGGY 2 cut(s) 997, 2048
Bse1I ACTGG 2 cut(s) 1127, 1985
Bse3DI GCAATG 3 cut(s) 69, 267, 930
Bse8I GATNNNNATC 1 cut(s) 290
BseAI TCCGGA 1 cut(s) 2131
BseCI ATCGAT 3 cut(s) 715, 2316, 2322
BseDI CCNNGG 5 cut(s) 447, 466, 1262, 1564, 2343
BseGI GGATG 6 cut(s) 934, 1465, 1495, 2011, 2140, 2405
BseJI GATNNNNATC 1 cut(s) 290
BseLI CCNNNNNNNGG 2 cut(s) 1570, 1713
BseMI GCAATG 3 cut(s) 69, 267, 930
BseMII CTCAG 3 cut(s) 138, 577, 1827
BseNI ACTGG 2 cut(s) 1127, 1985
BseRI GAGGAG 2 cut(s) 486, 1384
BsgI GTGCAG 1 cut(s) 2096
Bsh1285I CGRYCG 1 cut(s) 1002
BshFI GGCC 5 cut(s) 276, 446, 723, 1186, 1442
BshNI GGYRCC 2 cut(s) 1227, 2153
BshTI ACCGGT 1 cut(s) 997
BshVI ATCGAT 3 cut(s) 715, 2316, 2322
BsiEI CGRYCG 1 cut(s) 1002
BsiHKAI GWGCWC 1 cut(s) 562
BsiSI CCGG 6 cut(s) 998, 1262, 1287, 1582, 2049, 2132
BslFI GGGAC 1 cut(s) 55
BslI CCNNNNNNNGG 2 cut(s) 1570, 1713
BsmAI GTCTC 2 cut(s) 137, 450
BsmFI GGGAC 1 cut(s) 55
BsnI GGCC 5 cut(s) 276, 446, 723, 1186, 1442
Bsp119I TTCGAA 1 cut(s) 2288
Bsp1286I GDGCHC 1 cut(s) 562
Bsp13I TCCGGA 1 cut(s) 2131
Bsp143I GATC 8 cut(s) 34, 100, 716, 750, 1043, 1399, 1740, 2054
BspACI CCGC 3 cut(s) 915, 1955, 2157
BspANI GGCC 5 cut(s) 276, 446, 723, 1186, 1442
BspCNI CTCAG 3 cut(s) 139, 576, 1828
BspDI ATCGAT 3 cut(s) 715, 2316, 2322
BspEI TCCGGA 1 cut(s) 2131
BspHI TCATGA 1 cut(s) 1969
BspLI GGNNCC 4 cut(s) 191, 1229, 1983, 2155
BspMAI CTGCAG 2 cut(s) 316, 2173
BspPI GGATC 3 cut(s) 1051, 1735, 2062
BspT104I TTCGAA 1 cut(s) 2288
BspT107I GGYRCC 2 cut(s) 1227, 2153
BspTI CTTAAG 1 cut(s) 1322
BsrDI GCAATG 3 cut(s) 69, 267, 930
BsrFI RCCGGY 2 cut(s) 997, 2048
BsrI ACTGG 2 cut(s) 1127, 1985
BssAI RCCGGY 2 cut(s) 997, 2048
BssECI CCNNGG 5 cut(s) 447, 466, 1262, 1564, 2343
BssMI GATC 8 cut(s) 34, 100, 716, 750, 1043, 1399, 1740, 2054
BssSI CACGAG 1 cut(s) 52
BssT1I CCWWGG 2 cut(s) 466, 1564
Bst2BI CACGAG 1 cut(s) 52
Bst4CI ACNGT 8 cut(s) 641, 844, 864, 1147, 1197, 1614, 1692, 1929
Bst6I CTCTTC 1 cut(s) 2371
BstAFI CTTAAG 1 cut(s) 1322
BstAPI GCANNNNNTGC 2 cut(s) 320, 2086
BstBAI YACGTR 2 cut(s) 963, 1659
BstBI TTCGAA 1 cut(s) 2288
BstC8I GCNNGC 3 cut(s) 262, 274, 602
BstDEI CTNAG 5 cut(s) 147, 481, 563, 1836, 2218
BstDSI CCRYGG 2 cut(s) 447, 2343
BstF5I GGATG 6 cut(s) 934, 1465, 1495, 2011, 2140, 2405
BstKTI GATC 8 cut(s) 37, 103, 719, 753, 1046, 1402, 1743, 2057
BstMAI GTCTC 2 cut(s) 137, 450
BstMBI GATC 8 cut(s) 34, 100, 716, 750, 1043, 1399, 1740, 2054
BstMCI CGRYCG 1 cut(s) 1002
BstMWI GCNNNNNNNGC 6 cut(s) 273, 320, 2086, 2153, 2165, 2168
BstNSI RCATGY 3 cut(s) 585, 1057, 1871
BstSCI CCNGG 2 cut(s) 1261, 1580
BstSFI CTRYAG 2 cut(s) 312, 2169
BstV2I GAAGAC 1 cut(s) 483
BstX2I RGATCY 2 cut(s) 1740, 2054
BstYI RGATCY 2 cut(s) 1740, 2054
Bsu15I ATCGAT 3 cut(s) 715, 2316, 2322
BsuRI GGCC 5 cut(s) 276, 446, 723, 1186, 1442
BsuTUI ATCGAT 3 cut(s) 715, 2316, 2322
BtgI CCRYGG 2 cut(s) 447, 2343
BtgZI GCGATG 1 cut(s) 1808
BtrI CACGTC 1 cut(s) 541
BtsCI GGATG 6 cut(s) 934, 1465, 1495, 2011, 2140, 2405
BtsI GCAGTG 2 cut(s) 751, 2032
BtsIMutI CAGTG 5 cut(s) 621, 751, 1053, 1187, 2032
Cac8I GCNNGC 3 cut(s) 262, 274, 602
CaiI CAGNNNCTG 2 cut(s) 146, 623
CciI TCATGA 1 cut(s) 1969
Cfr10I RCCGGY 2 cut(s) 997, 2048
Cfr13I GGNCC 1 cut(s) 1860
ClaI ATCGAT 3 cut(s) 715, 2316, 2322
Csp6I GTAC 6 cut(s) 49, 393, 654, 944, 1148, 1228
CspAI ACCGGT 1 cut(s) 997
CspCI CAANNNNNGTGG 4 cut(s) 610, 645, 2332, 2367
CviQI GTAC 6 cut(s) 49, 393, 654, 944, 1148, 1228
DdeI CTNAG 5 cut(s) 147, 481, 563, 1836, 2218
DpnI GATC 8 cut(s) 36, 102, 718, 752, 1045, 1401, 1742, 2056
DpnII GATC 8 cut(s) 34, 100, 716, 750, 1043, 1399, 1740, 2054
DraIII CACNNNGTG 1 cut(s) 1730
DriI GACNNNNNGTC 2 cut(s) 1612, 1816
EaeI YGGCCR 1 cut(s) 1184
Eam1104I CTCTTC 1 cut(s) 2371
Eam1105I GACNNNNNGTC 2 cut(s) 1612, 1816
EarI CTCTTC 1 cut(s) 2371
Eco130I CCWWGG 2 cut(s) 466, 1564
Eco32I GATATC 2 cut(s) 1960, 2320
Eco47I GGWCC 1 cut(s) 1860
Eco57I CTGAAG 1 cut(s) 600
EcoRI GAATTC 1 cut(s) 2064
EcoRV GATATC 2 cut(s) 1960, 2320
EcoT14I CCWWGG 2 cut(s) 466, 1564
EcoT22I ATGCAT 1 cut(s) 1342
ErhI CCWWGG 2 cut(s) 466, 1564
FaqI GGGAC 1 cut(s) 55
FauI CCCGC 1 cut(s) 922
FauNDI CATATG 1 cut(s) 1621
FbaI TGATCA 2 cut(s) 100, 1399
FblI GTMKAC 1 cut(s) 1616
FokI GGATG 5 cut(s) 941, 1472, 1502, 2018, 2147
FspBI CTAG 4 cut(s) 555, 1824, 2061, 2407
GsuI CTGGAG 3 cut(s) 474, 1110, 2000
HaeIII GGCC 5 cut(s) 276, 446, 723, 1186, 1442
HapII CCGG 6 cut(s) 998, 1262, 1287, 1582, 2049, 2132
HindIII AAGCTT 1 cut(s) 2198
HinfI GANTC 7 cut(s) 164, 233, 896, 1152, 1353, 1484, 1840
HpaII CCGG 6 cut(s) 998, 1262, 1287, 1582, 2049, 2132
HphI GGTGA 9 cut(s) 173, 434, 455, 667, 1323, 1490, 1570, 1607, 2116
Hpy166II GTNNAC 4 cut(s) 538, 1051, 1617, 1936
Hpy188I TCNGA 4 cut(s) 1399, 1489, 1845, 2273
Hpy8I GTNNAC 4 cut(s) 538, 1051, 1617, 1936
HpyAV CCTTC 6 cut(s) 8, 145, 334, 436, 1272, 1996
HpyCH4III ACNGT 8 cut(s) 641, 844, 864, 1147, 1197, 1614, 1692, 1929
HpyCH4IV ACGT 6 cut(s) 47, 241, 540, 962, 1406, 1658
HpyF10VI GCNNNNNNNGC 6 cut(s) 273, 320, 2086, 2153, 2165, 2168
HpyF3I CTNAG 5 cut(s) 147, 481, 563, 1836, 2218
HpySE526I ACGT 6 cut(s) 47, 241, 540, 962, 1406, 1658
Kpn2I TCCGGA 1 cut(s) 2131
KpnI GGTACC 1 cut(s) 1231
Ksp22I TGATCA 2 cut(s) 100, 1399
Kzo9I GATC 8 cut(s) 34, 100, 716, 750, 1043, 1399, 1740, 2054
LmnI GCTCC 3 cut(s) 8, 976, 1981
LweI GCATC 5 cut(s) 276, 838, 868, 1996, 2390
MaeI CTAG 4 cut(s) 555, 1824, 2061, 2407
MaeII ACGT 6 cut(s) 47, 241, 540, 962, 1406, 1658
MalI GATC 8 cut(s) 36, 102, 718, 752, 1045, 1401, 1742, 2056
MboI GATC 8 cut(s) 34, 100, 716, 750, 1043, 1399, 1740, 2054
MfeI CAATTG 2 cut(s) 24, 2142
MflI RGATCY 2 cut(s) 1740, 2054
MhlI GDGCHC 1 cut(s) 562
MlsI TGGCCA 1 cut(s) 1186
MluNI TGGCCA 1 cut(s) 1186
MlyI GAGTC 2 cut(s) 905, 1161
MmeI TCCRAC 1 cut(s) 1621
Mox20I TGGCCA 1 cut(s) 1186
Mph1103I ATGCAT 1 cut(s) 1342
MroI TCCGGA 1 cut(s) 2131
MroXI GAANNNNTTC 1 cut(s) 1559
MscI TGGCCA 1 cut(s) 1186
MseI TTAA 3 cut(s) 572, 1026, 1323
MslI CAYNNNNRTG 1 cut(s) 954
Msp20I TGGCCA 1 cut(s) 1186
MspA1I CMGCKG 2 cut(s) 917, 2159
MspCI CTTAAG 1 cut(s) 1322
MspI CCGG 6 cut(s) 998, 1262, 1287, 1582, 2049, 2132
MspR9I CCNGG 2 cut(s) 1263, 1582
MunI CAATTG 2 cut(s) 24, 2142
MwoI GCNNNNNNNGC 6 cut(s) 273, 320, 2086, 2153, 2165, 2168
NciI CCSGG 2 cut(s) 1263, 1582
NdeI CATATG 1 cut(s) 1621
NdeII GATC 8 cut(s) 34, 100, 716, 750, 1043, 1399, 1740, 2054
NlaIV GGNNCC 4 cut(s) 191, 1229, 1983, 2155
NmeAIII GCCGAG 2 cut(s) 835, 1732
NmuCI GTSAC 6 cut(s) 1308, 1391, 1576, 1808, 2224, 2235
NsiI ATGCAT 1 cut(s) 1342
NspI RCATGY 3 cut(s) 585, 1057, 1871
NspV TTCGAA 1 cut(s) 2288
PagI TCATGA 1 cut(s) 1969
PciI ACATGT 1 cut(s) 1053
PcsI WCGNNNNNNNCGW 1 cut(s) 980
PdmI GAANNNNTTC 1 cut(s) 1559
PfeI GAWTC 5 cut(s) 164, 233, 1353, 1484, 1840
PinAI ACCGGT 1 cut(s) 997
PleI GAGTC 2 cut(s) 904, 1160
PpsI GAGTC 2 cut(s) 904, 1160
Ppu21I YACGTR 2 cut(s) 963, 1659
PscI ACATGT 1 cut(s) 1053
PspN4I GGNNCC 4 cut(s) 191, 1229, 1983, 2155
PspPI GGNCC 1 cut(s) 1860
PstI CTGCAG 2 cut(s) 316, 2173
PstNI CAGNNNCTG 2 cut(s) 146, 623
PsuI RGATCY 2 cut(s) 1740, 2054
RsaI GTAC 6 cut(s) 50, 394, 655, 945, 1149, 1229
RsaNI GTAC 6 cut(s) 49, 393, 654, 944, 1148, 1228
RseI CAYNNNNRTG 1 cut(s) 954
SaqAI TTAA 3 cut(s) 572, 1026, 1323
Sau3AI GATC 8 cut(s) 34, 100, 716, 750, 1043, 1399, 1740, 2054
Sau96I GGNCC 1 cut(s) 1860
SchI GAGTC 2 cut(s) 905, 1161
ScrFI CCNGG 2 cut(s) 1263, 1582
SduI GDGCHC 1 cut(s) 562
SfaNI GCATC 5 cut(s) 276, 838, 868, 1996, 2390
SfcI CTRYAG 2 cut(s) 312, 2169
SfuI TTCGAA 1 cut(s) 2288
SinI GGWCC 1 cut(s) 1860
SmiMI CAYNNNNRTG 1 cut(s) 954
SmlI CTYRAG 5 cut(s) 812, 1322, 1359, 2163, 2176
SmoI CTYRAG 5 cut(s) 812, 1322, 1359, 2163, 2176
SsiI CCGC 3 cut(s) 915, 1955, 2157
SspI AATATT 1 cut(s) 1559
SspMI CTAG 4 cut(s) 555, 1824, 2061, 2407
StyD4I CCNGG 2 cut(s) 1261, 1580
StyI CCWWGG 2 cut(s) 466, 1564
TaaI ACNGT 8 cut(s) 641, 844, 864, 1147, 1197, 1614, 1692, 1929
TaiI ACGT 6 cut(s) 50, 244, 543, 965, 1409, 1661
TaqI TCGA 6 cut(s) 636, 715, 870, 2288, 2316, 2322
TauI GCSGC 1 cut(s) 2159
TfiI GAWTC 5 cut(s) 164, 233, 1353, 1484, 1840
Tru1I TTAA 3 cut(s) 572, 1026, 1323
Tru9I TTAA 3 cut(s) 572, 1026, 1323
TscAI CASTG 5 cut(s) 628, 751, 1053, 1194, 2032
TseFI GTSAC 6 cut(s) 1308, 1391, 1576, 1808, 2224, 2235
Tsp45I GTSAC 6 cut(s) 1308, 1391, 1576, 1808, 2224, 2235
TspDTI ATGAA 8 cut(s) 597, 945, 1154, 1211, 1514, 1550, 1811, 2192
TspGWI ACGGA 4 cut(s) 989, 1056, 2311, 2360
TspRI CASTG 5 cut(s) 628, 751, 1053, 1194, 2032
Vha464I CTTAAG 1 cut(s) 1322
VpaK11BI GGWCC 1 cut(s) 1860
XapI RAATTY 5 cut(s) 686, 1174, 1526, 1540, 2064
XbaI TCTAGA 2 cut(s) 554, 2060
XceI RCATGY 3 cut(s) 585, 1057, 1871
XmiI GTMKAC 1 cut(s) 1616
XmnI GAANNNNTTC 1 cut(s) 1559
XspI CTAG 4 cut(s) 555, 1824, 2061, 2407
Zsp2I ATGCAT 1 cut(s) 1342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.