RchiOBHm_Chr2g0156071

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000071
Physical Location & Seq
Reverse (-)
72818970 .. 72823103
4134 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52491

Sequence Viewer

Length: 4134 bp
ATGACAGAAGGAGAAGGTTCTCTCGTCGCAAAGACAGAAGGCCCAGACGGGAATCCAAATCAACGTCTATGCTCAGTGCTTCTAAATGAGTTCAACTACCTTCCTTGGTCAAGAGCCATCACTCTAGCTCTTGGTGGAAGATCAAAGCTCAACTTCATCAATGGCAAAAACAACATCCCAGATGCCTCATCATCTGAATATGAAAGTTGGTTATCCAAAGACCAACTTGTTATGTCATGGCTTATTAATTCAATGGAGCCAAAACTTGCGGAAATCTTCAGCTATTCGGAGTCATCTCAACATCTTTGGGATGCTGTCAAAGACATGTATGGCAACCTAAACAATGCAGCAAGAGTGTTCCAACTCAAAAAAGATCTCGCAGGGATTCAACAAGGTAATCTATCATTTGTTCAACATCTTGGCAACTTAAAAGCCAAGTGGAATGAGCTTGATACATATAGGCCTCACACCATTGATGCCACCATATTGCTGAAAAGAGCTGAAGAAGATAAAGTGTTCCAACTACTTGCAAGTCTGGGATCTGAATATGAAGATCTAAAAAGTCATCTTTTGATAAGTCCTGAGCTTCCTTCGTTCACGACAGTATGCAACTCCATTCAACTTGGAGAAGTGCGCAAAAGGGTAATGAACGTGGATGCTAGTGCTGAAAGGTCAGAAGCTCGAGCATTTGTTGCAAATAAAAGTGTTACAAGTGATAGAACATACAAGGGCAAGAGGCCAGATCTGAAGTGCACTCATTGTGAACGCATTGGACGAACTGGTATAGGCCACACAAGAGAGAGATGTTGGATTTTGCATCCAGAACTGAAGCCTAAATTCAATGAGGACCAGAAGAATCAAAGAGGCACCATTCAGAAAAGCTCTTACATCTCTAATCCAAAGGCAAACTTCAGCAACATTTCTGAAGATATGATGAACTTCACGTCCAATCCAATAACTCTCATAAATGAGTTTGCTACATATCTTCAAAAGAAGCAAGACAGTTTAGAGAGCAATGAAAATGGAAGCACAACTGCTATGCTTGGAAAGTTTGCGGGTTTTCTAGCAAAATCAAATATGGCTTCTTCAGAGGATATCCCAGGTATTATTTGCGCTATTTCTACTGCTTTAGATGTCAGTAAGAATCATGATTTTTGGATAGTGGATTCAGGTGCCTCAGATCACATGACAAACAATTCTTTCATTTTACATGATTTTGAAACTCTGTCAAAACCTTCACATGTCTCAATTGCAAATGGCAACGATGTTCCCATCTTAGGAAAAGGAAAACTAAAGCTTTTTTCAGATAGTGTTGAGTCATTTGCACTATTTGTACCTTCTTTTCCATTCCAACTCCTGTCTGTAGGAAGGTTAATGAACTCATTGGATTGTCTAGCCATTTTCTCTCCTTATAATGTTGTGTTTCAGGATCGAGTGTCCAAGAAGAAGATTGGTGAAGGGTTCTTTCTAAATGGCTTGTACTACATATCAACTTCATCAAGCTTTTCAAAGTGTTTTCTGACAGAATCCAAATCTGCAACACAACAACAATTGTGGCATAAACGCCTAGCTCATCCTTCCTTTCATGTTTTATCAATATTGTTCCCTAGTTTTTGTAAAGTTTCTCATGAATGTGAAACATGTCATATGTCAAAGTTTACTAGATTGCCTTTTCAAATCTCTCAATCTAGAACAACTCAGCCTTTTGAGATCGTACATTCTGATGTATGGGGACCAGCATCCCTAGAATCGTTTGATGGTTATAGGTTTTATGTCACCTTTATTGATGATTTCACTCGAACCACTTTTGTGTATCTTTTGAAATTCAAGAGCGAAGTCTTTAAGTGTTTTCAAGATTTTCATAACCTTGTTAAAAATCATTTTTCATCTAAAATTTGCATTCTAAGGTCAGATAATGGAACTGAATACACATCCAAAATCATGACTGATTATCTCAGTGCTCAAGGGATTATTCATCACACCAGTTGTGTGGGAACACCACAACAAAATGGCATTGCAGAACGCAAAAATAGAGATTTACTTGAGAAGGCTAGGGCCTTAATGCTTCAAACAAATGTTCCAAAAAAATTCTGGTCTCAAGGAATTCAAACTGCAGCTTACATTATAAACAGATTGCCTAGCAGTGTGTTAAATTTCAGATCTCCATTTGAAGTTCTAAAAGGTCGAAAGGTTGACATAACACACCTTCGAGTATTTGGTTGTACGTGTTTTGTACATGTTCAGTCAAATCACCGAGATAAGTTTGATCCCAGAGCTATCAAATGTGTGTTCCTCGGGTACTCATCTACCCAAAAGGGCTACAAGTGTTACAATTCACAGACCAGAAAATTCTTTGTTTCAAGGGATGTTCGTTTTGATGAGTCTGACTCTTTCTTTCAGTTATCTGACAATGAACCTCAGGGGGAGCATATTTGTGACTTGTTTCCCACTCCAATTCCTGTTGAAGCTGCAGTAGACACTCCTAGTTCACAACAACCAGTTCAACAAATTGTGGACACTGAAGAGGTACAGGCAGACTCCAACACTCAAGATCATCAACCAGAGGTTGTACCAAACACGCCTCAACCTCGAAGAAACCCACCAAGGGGGAGACATCTACCAGCAAGATTTCAGGAATATGAGACTTACACACCACGTTATCCTCTTGCTCAAGTCGCTCACTACACCAGCACCTCCTCTCCTCATAGTGCATTCCTCATCAAGATTTCCAAAGAAACCGAACCAAGGAACTTTGAAGAAGCAAATCAATCCCCAGTTTGGAAGAAAGCTATGGATGATGAACTCAAAGCGCTCGATGACAATAGAACCTGGAGTATAGTTAAGCTTCCCAAAGGACAGAAAATTGTAGGGGCAAGATGGATTTACAAAATCAAGTTCAACTCCGATGGTTCAATTGAGCGACATAAGGCAAGGTTAGTAGCTCGTGGCTTCACTCAAACCTTTGGTGTTGATTATAAGGAGACCTTTGCTCCAGTTGCTAAAATGAACACAGTTCGAGTTTTGTTATCTGTTGCTGTGAATTGTGGATGGTCACTATACCAAATGGATGTCAAGAATGCCTTTTTGCATGGGGATCTAGAAGAAGATGTGTATATGAGATTACCTCCTGGACATCCACGTGAAAGTGAAGCTGGAATGGTGTGCAAACTTCACAAGGCCTTGTATGGTTTGAAACAATCCCCACGTGCTTGGTATTCCAAATTGAGTTCGGTTCTTCTTGCCTCAAGCTTCAAGAGAAGTCATGCAGACTCCTCCCTATTTATTCGAAATGGCAAGGCTGGCAAGTTGGTTGTTCTTGTGTATGTGGATGACTTAATCATCACTGGAGACAATATGGATGAGATTAAGTCTCTCAAGTCAGCCTTGCACAATACCTTTGCTATCAAAGATCTTGGCCCATTGAAGTATTTTCTTGGAATTGAAATGGATCACTCCTCAAATGGTATATTCTTAAATCAACGCAAATATGTTGTTGATCTTCTTGATGAGGCAGGAATGAAGGATAGTAAGCCTGCACACACTCCTCTTAGTAGCAGACTTAAAATAGATATTGAAGGAGAACCTCTCTCAGATATTTGTGTTTATCAACGCCTTGTGGGTAAGCTCATCTATCTCACCATCACAAGACCTGACCTCACTTATGCTGTAAGTTTGGTCAGTCAATTCATGCACTCCCCTACCACTCATCATCTACAGATTGTCAAGAGGATCCTTCGCTACTTAAAGGGCACAGTTGATCGAGGAATTGTTATGAAGAACAATGGTCACTTCAACCTTGTGGGCTACTCAGACTCTGACTGGGCAGGAAATGCCATCGATCGAAAATCCACAACTGGGTACTGTACTTTCATTGGAGGCAACCTAGTTACTTGGAAAAGTAAAAAACAAACTGTGGTTGCTTGTTCTAGTGCCGAAGCTGAATATCGTGCAATGGCATCCACAGCCTGTGAACTCATATGGTTAAAGACATTGTTAGGTGACTTGGGCATTGTGTGCACTTTGCCTATATGTCTACATTGTGACAACCAGGCTGCCATGCATATCGCAGCCAATCCAGTCTTCCATGAACGCACCAAACATATTGAAGTTGATTGTCACTTTGTTCGAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

1377

Amino Acids

155.52

Weight (kDa)

8.66

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_3 PF14244 19 - 60 4.8e-11 gag-polypeptide of LTR copia-type
Retrotran_gag_2 PF14223 52 - 214 3.5e-09 gag-polypeptide of LTR copia-type
Pol_BBD PF22936 386 - 460 3.4e-13 Pol polyprotein, beta-barrel domain
gag_pre-integrs PF13976 492 - 552 1.2e-08 GAG-pre-integrase domain
rve PF00665 566 - 667 4.3e-09 Integrase core domain
SH3_retrovirus PF25597 741 - 803 4e-28 Retroviral polymerase SH3-like domain
RVT_2 PF07727 941 - 1183 2.8e-78 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1413, 2126, 2976
Acc16I TGCGCA 1 cut(s) 635
AccB1I GGYRCC 2 cut(s) 866, 1172
AccI GTMKAC 2 cut(s) 2475, 4033
AciI CCGC 2 cut(s) 269, 1055
AclWI GGATC 7 cut(s) 547, 1437, 2261, 3102, 3456, 3724, 3737
AcsI RAATTY 7 cut(s) 836, 1823, 1893, 2087, 2103, 2152, 2348
AcuI CTGAAG 8 cut(s) 262, 522, 767, 848, 895, 945, 1071, 2541
AcvI CACGTG 2 cut(s) 3140, 3206
AfeI AGCGCT 1 cut(s) 2811
AfiI CCNNNNNNNGG 6 cut(s) 1277, 2605, 2606, 2745, 2778, 3855
AflIII ACRYGT 5 cut(s) 324, 1240, 1640, 2225, 2236
AjiI CACGTC 1 cut(s) 945
AjnI CCWGG 4 cut(s) 1099, 2828, 3127, 4047
AjuI GAANNNNNNNTTGG 2 cut(s) 4064, 4096
AleI CACNNNNGTG 1 cut(s) 1808
AloI GAACNNNNNNTCC 2 cut(s) 929, 961
Alw21I GWGCWC 3 cut(s) 755, 1963, 4019
Alw26I GTCTC 7 cut(s) 1249, 2100, 2605, 2636, 2975, 3342, 3375
Alw44I GTGCAC 2 cut(s) 751, 4015
AlwI GGATC 7 cut(s) 547, 1437, 2261, 3102, 3456, 3724, 3737
AlwNI CAGNNNCTG 1 cut(s) 3815
Ama87I CYCGRG 2 cut(s) 681, 2294
Aor51HI AGCGCT 1 cut(s) 2811
AoxI GGCC 7 cut(s) 40, 461, 737, 787, 2055, 3177, 3415
ApaLI GTGCAC 2 cut(s) 751, 4015
ApeKI GCWGC 5 cut(s) 347, 2114, 2468, 4052, 4067
ApoI RAATTY 7 cut(s) 836, 1823, 1893, 2087, 2103, 2152, 2348
AseI ATTAAT 1 cut(s) 246
AspLEI GCGC 3 cut(s) 636, 1115, 2812
AspS9I GGNCC 5 cut(s) 41, 847, 1733, 2055, 3416
AsuHPI GGTGA 5 cut(s) 1466, 1768, 2243, 3628, 4010
AsuII TTCGAA 1 cut(s) 3286
AvaI CYCGRG 2 cut(s) 681, 2294
AvaII GGWCC 2 cut(s) 847, 1733
AxyI CCTNAGG 1 cut(s) 2418
BaeGI GKGCMC 3 cut(s) 755, 3752, 4019
BamHI GGATCC 1 cut(s) 3729
BanI GGYRCC 2 cut(s) 866, 1172
BauI CACGAG 1 cut(s) 2943
BbrPI CACGTG 2 cut(s) 3140, 3206
BbsI GAAGAC 1 cut(s) 4072
Bbv12I GWGCWC 3 cut(s) 755, 1963, 4019
BbvI GCAGC 5 cut(s) 359, 2126, 2455, 4039, 4079
BccI CCATC 8 cut(s) 125, 1280, 1751, 2871, 2900, 3042, 3647, 3842
BciT130I CCWGG 4 cut(s) 1101, 2830, 3129, 4049
BcoDI GTCTC 7 cut(s) 1249, 2100, 2605, 2636, 2975, 3342, 3375
BfmI CTRYAG 4 cut(s) 1362, 2112, 2469, 3713
BfoI RGCGCY 1 cut(s) 2813
BglII AGATCT 5 cut(s) 373, 553, 742, 2159, 3409
BisI GCNGC 5 cut(s) 348, 2115, 2469, 4053, 4068
BlsI GCNGC 5 cut(s) 349, 2116, 2470, 4054, 4069
Bme1390I CCNGG 4 cut(s) 1101, 2830, 3129, 4049
Bme18I GGWCC 2 cut(s) 847, 1733
BmeT110I CYCGRG 2 cut(s) 681, 2294
BmgBI CACGTC 1 cut(s) 945
BmgT120I GGNCC 5 cut(s) 41, 847, 1733, 2055, 3416
BmiI GGNNCC 5 cut(s) 258, 868, 1174, 1734, 3731
BmrFI CCNGG 4 cut(s) 1101, 2830, 3129, 4049
BmrI ACTGGG 3 cut(s) 2768, 3829, 3864
BmsI GCATC 7 cut(s) 172, 301, 466, 646, 826, 1748, 3965
BmuI ACTGGG 3 cut(s) 2768, 3829, 3864
BpiI GAAGAC 1 cut(s) 4072
BplI GAGNNNNNCTC 6 cut(s) 2372, 2404, 3109, 3141, 3570, 3602
BpmI CTGGAG 3 cut(s) 2851, 2976, 3366
Bpu10I CCTNAGC 1 cut(s) 582
Bpu14I TTCGAA 1 cut(s) 3286
BpuEI CTTGAG 7 cut(s) 1947, 2063, 2082, 2532, 2655, 3229, 3359
Bsa29I ATCGAT 1 cut(s) 3837
BsaAI YACGTR 3 cut(s) 2226, 3140, 3206
BsaI GGTCTC 2 cut(s) 2100, 2975
BsaJI CCNNGG 5 cut(s) 104, 1099, 2293, 2603, 2744
BsaXI ACNNNNNCTCC 4 cut(s) 2683, 2713, 2974, 3004
Bsc4I CCNNNNNNNGG 6 cut(s) 1277, 2605, 2606, 2745, 2778, 3855
Bse1I ACTGG 9 cut(s) 784, 1983, 2498, 2774, 2993, 3349, 3824, 3859, 4076
Bse21I CCTNAGG 1 cut(s) 2418
Bse3DI GCAATG 3 cut(s) 1021, 2013, 3957
BseBI CCWGG 4 cut(s) 1101, 2830, 3129, 4049
BseCI ATCGAT 1 cut(s) 3837
BseDI CCNNGG 5 cut(s) 104, 1099, 2293, 2603, 2744
BseLI CCNNNNNNNGG 6 cut(s) 1277, 2605, 2606, 2745, 2778, 3855
BseMI GCAATG 3 cut(s) 1021, 2013, 3957
BseMII CTCAG 8 cut(s) 87, 573, 1191, 1712, 1969, 2432, 3603, 3822
BseNI ACTGG 9 cut(s) 784, 1983, 2498, 2774, 2993, 3349, 3824, 3859, 4076
BseRI GAGGAG 5 cut(s) 2686, 2691, 3262, 3445, 3534
BseSI GKGCMC 3 cut(s) 755, 3752, 4019
BseXI GCAGC 5 cut(s) 359, 2126, 2455, 4039, 4079
BsgI GTGCAG 1 cut(s) 3519
Bsh1285I CGRYCG 1 cut(s) 3841
BshFI GGCC 7 cut(s) 42, 463, 739, 789, 2057, 3179, 3417
BshNI GGYRCC 2 cut(s) 866, 1172
BshVI ATCGAT 1 cut(s) 3837
BsiEI CGRYCG 1 cut(s) 3841
BsiHKAI GWGCWC 3 cut(s) 755, 1963, 4019
BsiHKCI CYCGRG 2 cut(s) 681, 2294
BslFI GGGAC 1 cut(s) 1746
BslI CCNNNNNNNGG 6 cut(s) 1277, 2605, 2606, 2745, 2778, 3855
BsmAI GTCTC 7 cut(s) 1249, 2100, 2605, 2636, 2975, 3342, 3375
BsmFI GGGAC 1 cut(s) 1746
BsmI GAATGC 3 cut(s) 1899, 2711, 3082
BsnI GGCC 7 cut(s) 42, 463, 739, 789, 2057, 3179, 3417
Bso31I GGTCTC 2 cut(s) 2100, 2975
BsoBI CYCGRG 2 cut(s) 681, 2294
Bsp119I TTCGAA 1 cut(s) 3286
Bsp1286I GDGCHC 4 cut(s) 755, 1963, 3752, 4019
Bsp1407I TGTACA 1 cut(s) 2233
BspACI CCGC 2 cut(s) 269, 1055
BspANI GGCC 7 cut(s) 42, 463, 739, 789, 2057, 3179, 3417
BspCNI CTCAG 8 cut(s) 86, 574, 1190, 1711, 1968, 2431, 3602, 3821
BspDI ATCGAT 1 cut(s) 3837
BspHI TCATGA 3 cut(s) 1147, 1627, 1941
BspLI GGNNCC 5 cut(s) 258, 868, 1174, 1734, 3731
BspMAI CTGCAG 2 cut(s) 2116, 2473
BspPI GGATC 7 cut(s) 547, 1437, 2261, 3102, 3456, 3724, 3737
BspT104I TTCGAA 1 cut(s) 3286
BspT107I GGYRCC 2 cut(s) 866, 1172
BspTNI GGTCTC 2 cut(s) 2100, 2975
BsrDI GCAATG 3 cut(s) 1021, 2013, 3957
BsrGI TGTACA 1 cut(s) 2233
BsrI ACTGG 9 cut(s) 784, 1983, 2498, 2774, 2993, 3349, 3824, 3859, 4076
BssECI CCNNGG 5 cut(s) 104, 1099, 2293, 2603, 2744
BssSI CACGAG 1 cut(s) 2943
BssT1I CCWWGG 3 cut(s) 104, 2603, 2744
Bst2BI CACGAG 1 cut(s) 2943
Bst2UI CCWGG 4 cut(s) 1101, 2830, 3129, 4049
Bst4CI ACNGT 6 cut(s) 604, 1004, 3013, 3754, 3863, 3913
Bst6I CTCTTC 1 cut(s) 2517
BstAPI GCANNNNNTGC 3 cut(s) 692, 3830, 4014
BstAUI TGTACA 1 cut(s) 2233
BstBAI YACGTR 3 cut(s) 2226, 3140, 3206
BstBI TTCGAA 1 cut(s) 3286
BstC8I GCNNGC 2 cut(s) 3301, 3534
BstH2I RGCGCY 1 cut(s) 2813
BstHHI GCGC 3 cut(s) 636, 1115, 2812
BstMAI GTCTC 7 cut(s) 1249, 2100, 2605, 2636, 2975, 3342, 3375
BstMCI CGRYCG 1 cut(s) 3841
BstMWI GCNNNNNNNGC 7 cut(s) 692, 2675, 2996, 3300, 3830, 3962, 4014
BstNI CCWGG 4 cut(s) 1101, 2830, 3129, 4049
BstNSI RCATGY 4 cut(s) 328, 1244, 1644, 2240
BstSCI CCNGG 4 cut(s) 1099, 2828, 3127, 4047
BstSFI CTRYAG 4 cut(s) 1362, 2112, 2469, 3713
BstSLI GKGCMC 3 cut(s) 755, 3752, 4019
BstV1I GCAGC 5 cut(s) 359, 2126, 2455, 4039, 4079
BstV2I GAAGAC 1 cut(s) 4072
BstX2I RGATCY 8 cut(s) 373, 539, 553, 742, 2159, 3094, 3409, 3729
BstXI CCANNNNNNTGG 2 cut(s) 1990, 3210
BstYI RGATCY 8 cut(s) 373, 539, 553, 742, 2159, 3094, 3409, 3729
Bsu15I ATCGAT 1 cut(s) 3837
Bsu36I CCTNAGG 1 cut(s) 2418
BsuRI GGCC 7 cut(s) 42, 463, 739, 789, 2057, 3179, 3417
BsuTUI ATCGAT 1 cut(s) 3837
BtrI CACGTC 1 cut(s) 945
BtsI GCAGTG 1 cut(s) 2149
BtsIMutI CAGTG 5 cut(s) 81, 1963, 2149, 2517, 3342
Cac8I GCNNGC 2 cut(s) 3301, 3534
CaiI CAGNNNCTG 1 cut(s) 3815
CciI TCATGA 3 cut(s) 1147, 1627, 1941
CfoI GCGC 3 cut(s) 636, 1115, 2812
Cfr13I GGNCC 5 cut(s) 41, 847, 1733, 2055, 3416
ClaI ATCGAT 1 cut(s) 3837
CspCI CAANNNNNGTGG 2 cut(s) 1535, 1570
Eam1104I CTCTTC 1 cut(s) 2517
EarI CTCTTC 1 cut(s) 2517
Eco130I CCWWGG 3 cut(s) 104, 2603, 2744
Eco147I AGGCCT 2 cut(s) 463, 3179
Eco31I GGTCTC 2 cut(s) 2100, 2975
Eco32I GATATC 1 cut(s) 1096
Eco47I GGWCC 2 cut(s) 847, 1733
Eco47III AGCGCT 1 cut(s) 2811
Eco57I CTGAAG 8 cut(s) 262, 522, 767, 848, 895, 945, 1071, 2541
Eco72I CACGTG 2 cut(s) 3140, 3206
Eco81I CCTNAGG 1 cut(s) 2418
Eco88I CYCGRG 2 cut(s) 681, 2294
EcoO109I RGGNCCY 1 cut(s) 2055
EcoRI GAATTC 1 cut(s) 2103
EcoRII CCWGG 4 cut(s) 1099, 2828, 3127, 4047
EcoRV GATATC 1 cut(s) 1096
EcoT14I CCWWGG 3 cut(s) 104, 2603, 2744
EcoT22I ATGCAT 1 cut(s) 4062
ErhI CCWWGG 3 cut(s) 104, 2603, 2744
FaqI GGGAC 1 cut(s) 1746
FauI CCCGC 1 cut(s) 1048
FauNDI CATATG 2 cut(s) 1647, 3977
FblI GTMKAC 2 cut(s) 2475, 4033
Fnu4HI GCNGC 5 cut(s) 348, 2115, 2469, 4053, 4068
Fsp4HI GCNGC 5 cut(s) 348, 2115, 2469, 4053, 4068
FspI TGCGCA 1 cut(s) 635
GlaI GCGC 3 cut(s) 635, 1114, 2811
GluI GCNGC 5 cut(s) 348, 2115, 2469, 4053, 4068
GsuI CTGGAG 3 cut(s) 2851, 2976, 3366
HaeII RGCGCY 1 cut(s) 2813
HaeIII GGCC 7 cut(s) 42, 463, 739, 789, 2057, 3179, 3417
HhaI GCGC 3 cut(s) 636, 1115, 2812
Hin6I GCGC 3 cut(s) 634, 1113, 2810
HinP1I GCGC 3 cut(s) 634, 1113, 2810
HincII GTYRAC 1 cut(s) 2194
HindII GTYRAC 1 cut(s) 2194
HindIII AAGCTT 4 cut(s) 1295, 1501, 2843, 3247
HphI GGTGA 5 cut(s) 1466, 1768, 2243, 3628, 4010
Hpy99I CGWCG 1 cut(s) 29
HpyCH4III ACNGT 6 cut(s) 604, 1004, 3013, 3754, 3863, 3913
HpyCH4IV ACGT 7 cut(s) 64, 651, 944, 2225, 2656, 3139, 3205
HpyF10VI GCNNNNNNNGC 7 cut(s) 692, 2675, 2996, 3300, 3830, 3962, 4014
HpySE526I ACGT 7 cut(s) 64, 651, 944, 2225, 2656, 3139, 3205
HspAI GCGC 3 cut(s) 634, 1113, 2810
LmnI GCTCC 3 cut(s) 256, 2425, 2995
Lsp1109I GCAGC 5 cut(s) 359, 2126, 2455, 4039, 4079
LweI GCATC 7 cut(s) 172, 301, 466, 646, 826, 1748, 3965
MaeII ACGT 7 cut(s) 64, 651, 944, 2225, 2656, 3139, 3205
MfeI CAATTG 3 cut(s) 1248, 1550, 2913
MflI RGATCY 8 cut(s) 373, 539, 553, 742, 2159, 3094, 3409, 3729
MhlI GDGCHC 4 cut(s) 755, 1963, 3752, 4019
MlyI GAGTC 7 cut(s) 299, 1325, 2381, 2390, 2531, 3263, 3806
MmeI TCCRAC 5 cut(s) 385, 544, 788, 1375, 2565
Mph1103I ATGCAT 1 cut(s) 4062
MslI CAYNNNNRTG 5 cut(s) 1632, 1722, 1808, 2433, 3138
MspR9I CCNGG 4 cut(s) 1101, 2830, 3129, 4049
MunI CAATTG 3 cut(s) 1248, 1550, 2913
Mva1269I GAATGC 3 cut(s) 1899, 2711, 3082
MvaI CCWGG 4 cut(s) 1101, 2830, 3129, 4049
MwoI GCNNNNNNNGC 7 cut(s) 692, 2675, 2996, 3300, 3830, 3962, 4014
NdeI CATATG 2 cut(s) 1647, 3977
NlaIV GGNNCC 5 cut(s) 258, 868, 1174, 1734, 3731
NmuCI GTSAC 7 cut(s) 1774, 2435, 3051, 3785, 3998, 4040, 4115
NsbI TGCGCA 1 cut(s) 635
NsiI ATGCAT 1 cut(s) 4062
NspI RCATGY 4 cut(s) 328, 1244, 1644, 2240
NspV TTCGAA 1 cut(s) 3286
OliI CACNNNNGTG 1 cut(s) 1808
PaeR7I CTCGAG 1 cut(s) 681
PagI TCATGA 3 cut(s) 1147, 1627, 1941
PceI AGGCCT 2 cut(s) 463, 3179
PciI ACATGT 4 cut(s) 324, 1240, 1640, 2236
PcsI WCGNNNNNNNCGW 1 cut(s) 772
PctI GAATGC 3 cut(s) 1899, 2711, 3082
PfeI GAWTC 7 cut(s) 52, 385, 856, 1144, 1166, 1526, 1748
PfoI TCCNGGA 1 cut(s) 3127
PkrI GCNGC 5 cut(s) 349, 2116, 2470, 4054, 4069
Ple19I CGATCG 1 cut(s) 3841
PleI GAGTC 7 cut(s) 298, 1324, 2381, 2389, 2531, 3263, 3806
PmaCI CACGTG 2 cut(s) 3140, 3206
PmlI CACGTG 2 cut(s) 3140, 3206
PpsI GAGTC 7 cut(s) 298, 1324, 2381, 2389, 2531, 3263, 3806
Ppu21I YACGTR 3 cut(s) 2226, 3140, 3206
PscI ACATGT 4 cut(s) 324, 1240, 1640, 2236
PshBI ATTAAT 1 cut(s) 246
PsiI TTATAA 3 cut(s) 1413, 2126, 2976
Psp6I CCWGG 4 cut(s) 1099, 2828, 3127, 4047
PspCI CACGTG 2 cut(s) 3140, 3206
PspGI CCWGG 4 cut(s) 1099, 2828, 3127, 4047
PspN4I GGNNCC 5 cut(s) 258, 868, 1174, 1734, 3731
PspPI GGNCC 5 cut(s) 41, 847, 1733, 2055, 3416
PspXI VCTCGAGB 1 cut(s) 681
PstI CTGCAG 2 cut(s) 2116, 2473
PstNI CAGNNNCTG 1 cut(s) 3815
PsuI RGATCY 8 cut(s) 373, 539, 553, 742, 2159, 3094, 3409, 3729
PvuI CGATCG 1 cut(s) 3841
RseI CAYNNNNRTG 5 cut(s) 1632, 1722, 1808, 2433, 3138
SatI GCNGC 5 cut(s) 348, 2115, 2469, 4053, 4068
Sau96I GGNCC 5 cut(s) 41, 847, 1733, 2055, 3416
SchI GAGTC 7 cut(s) 299, 1325, 2381, 2390, 2531, 3263, 3806
ScrFI CCNGG 4 cut(s) 1101, 2830, 3129, 4049
SduI GDGCHC 4 cut(s) 755, 1963, 3752, 4019
SfaNI GCATC 7 cut(s) 172, 301, 466, 646, 826, 1748, 3965
SfcI CTRYAG 4 cut(s) 1362, 2112, 2469, 3713
Sfr274I CTCGAG 1 cut(s) 681
SfuI TTCGAA 1 cut(s) 3286
SinI GGWCC 2 cut(s) 847, 1733
SlaI CTCGAG 1 cut(s) 681
SmiMI CAYNNNNRTG 5 cut(s) 1632, 1722, 1808, 2433, 3138
SmlI CTYRAG 8 cut(s) 681, 1962, 2042, 2097, 2547, 2670, 3244, 3374
SmoI CTYRAG 8 cut(s) 681, 1962, 2042, 2097, 2547, 2670, 3244, 3374
SseBI AGGCCT 2 cut(s) 463, 3179
SsiI CCGC 2 cut(s) 269, 1055
SspI AATATT 1 cut(s) 1599
StuI AGGCCT 2 cut(s) 463, 3179
StyD4I CCNGG 4 cut(s) 1099, 2828, 3127, 4047
StyI CCWWGG 3 cut(s) 104, 2603, 2744
TaaI ACNGT 6 cut(s) 604, 1004, 3013, 3754, 3863, 3913
TaiI ACGT 7 cut(s) 67, 654, 947, 2228, 2659, 3142, 3208
TatI WGTACW 3 cut(s) 1479, 2233, 3863
TfiI GAWTC 7 cut(s) 52, 385, 856, 1144, 1166, 1526, 1748
TscAI CASTG 5 cut(s) 81, 1963, 2149, 2524, 3349
TseFI GTSAC 7 cut(s) 1774, 2435, 3051, 3785, 3998, 4040, 4115
TseI GCWGC 5 cut(s) 347, 2114, 2468, 4052, 4067
Tsp45I GTSAC 7 cut(s) 1774, 2435, 3051, 3785, 3998, 4040, 4115
TspRI CASTG 5 cut(s) 81, 1963, 2149, 2524, 3349
VneI GTGCAC 2 cut(s) 751, 4015
VpaK11BI GGWCC 2 cut(s) 847, 1733
VspI ATTAAT 1 cut(s) 246
XapI RAATTY 7 cut(s) 836, 1823, 1893, 2087, 2103, 2152, 2348
XbaI TCTAGA 2 cut(s) 1688, 3097
XceI RCATGY 4 cut(s) 328, 1244, 1644, 2240
XcmI CCANNNNNNNNNTGG 1 cut(s) 2088
XhoI CTCGAG 1 cut(s) 681
XmiI GTMKAC 2 cut(s) 2475, 4033
Zsp2I ATGCAT 1 cut(s) 4062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.