RchiOBHm_Chr3g0448011

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000071
Physical Location & Seq
Forward (+)
438215 .. 441168
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41546

Sequence Viewer

Length: 2616 bp
ATGAGGTATTTTGTATTGTTCATTGATGATTGTACGAGATTATCATGGGTTGCTCTTCTTAGAACCAAAGATGAAGTCTTTCCAGCCTTTCAAACTTTTCGTACTCTTGTTCAAACACAATACAATAGCACCATTCGAGTCATACGATCTGACAATGGGGGAGAGTATGTTAATCATGTTTTCAACAAATTTTTGCAAACACATGGCATTATTCACCAAACTACATGCCCACAGACTCCAGAACAAAATGGAGTGTCAGAACGGAAAAACCGTCATTTACTTGATATGGCTCGTGCCCTTCTCTTTAGTGCTCATATGCCTAAGTATCTTTGGGGCGATGCTGTACAAGCCTCCTCCTATCTTATCAATCGTCTTCCATCTAGTGTCCTTCAGGGAAAAATTCCATTTGAGGTCCTAGCATCTCATGTCTCCTTGCCATCATTTCATAATCTTCCTGCCTGTGTTTTTGGTTGTGTGGCCTTTGTTCATGTTCCTAAAAATCATAGGTCTAAGTTGGATGCCCGGGCAATTAAATGTGTGTTTGTTGGTTATGGCGGATATCAGAAAGGGTACAAGTGCTACCATCCACCTACCAGAAAGTACTATGTTACTATGGATGTTACTTTCTTTGAGGACATGAGTTATTTTTCCTCATCTGATACCGCTCTTCAGGGGGAGAATTCATACTTTGAAGAACTGTATCAGGGTGAGGGGGAGGAATTGAAAAGAAGCGAAGAAGTAACACAAGAAGGAGATATTTCAGCGGGTCAAGTTGAGATCCCTAATCCATCAAATTCAGTAGTCAAAGAAGCAGAAGCTCCAAGCATCCAGGCACCAGTTTCAATCACTGAAACTGAAGCTGAAGACACAACTGTCCCCTCAATCGTTGCTCCTACCCCTGACCCACAGCTCTCTGGCATTGAAGATCAGTCATCTGAGGTATGTTCACCTACTGGTACTAATAGTAGTGAGTCCAATGATGAACAGTATGTGTTGCCAAAAAGGTCTACTCGAGGTCAACCACCCAAAAGATATGAACCCACTGTTCATGCAAAATCAAAGTACCTAGTAGCTAATTACATGTCCACTAGGAGATTGTCTAAGTCATATGCATCATTTGTGAATCAAATATCTGCTGTATCAGTACCTAACAAAGTACATGATGCGTTGGGGGATCCAAAGTGGAGGAAGGCAATGGAAGAAGAGATGGAGGCGTTACAGAAGAACGAAACTTGGCAGCTTGTGCCTTCACCACAAGGCAAGAAGGCTGTAGGTTGTCGTTGGGTGTTCACTGTCAAACATAATGCAGATGGATCAGTGAATCGATATAAAGCACGCCTTGTAGCAAAAGGGTTTACTCAGACATATGGTATAGACTACGATGAGACTTTTGCTCCTGTTGCCAAAATGAACACTATTCGGGTTCTGCTTTCCTGTGTTGCTAATTTAAATTGGCCACTCCGACAGTTTGATGTCAAGAATGCATTTCTTCATGGAGAGTTAGCTGAGGAGGTATACATGAGTCTTCCACCTGGGTATGTAGCTGCTTCTCCTGGTGATTTTGTATGCAAATTAAGAAAATCTCTGTATGGTCTCAAACAGTCACCTCGCGCTTGGTTTGGGAGATTTTCACAGTTCATGAGGAAGTTTGGTTACAAACAGAGTAACTCAGACCACACCTTATTTCTCAAACATCAACAAGGGAAGGTAACAGCCCTAATCATTTATGTTGATGATATGGTAATCACTGGCAATGATACTATTGAGATGGATATACTACAGAGACAGCTAGCCTCTGAGTTCGAGATGAAGGACTTGGGCGAACTAAAGTACTTCTTAGGAATTGAGGTAGCCAGGGGGAGAGAAGGGATTTACTTGTGCCAGAGGAAGTATATTCTTGACCTATTGACAGAGACAGGTATGTTGGATTGCAAACCTATTGACACTCCTATTGAGCAAAACCATTGTTTAGCAGAATATCCAGATCAAGTACCTACTGACAGAGCTGGCTATCAGAGGTTGGTTGGGCGCTTGATTTATTTGGCTCATACCAGGCCAGATGTTGCATATGCAGTAAGTGTGGTAAGTCAGTTCATGCATAACCCGAGTGAGAGTCACATGGATGCTGTTATGAGAATTTTAAGGTACTTGAAGTCAGCTCCAGGGAGAGGAGTAATGTTTTCTAAACACAACAGCATTCTTGAGGTTTGTGGCTTCACAGATGCTGATTGGGCTGGAAATATCACAGATAGGAGGTCAACATCAGGTTACTTTACCTTTGTGGGAGGTAATTTAGTTACATGGAAGAGTAAGAAACAAAAAGTTGTGGCGCGATCCAGTGCTGAGGCTGAGTATAGAGGTATGGCTCATGGGGTGTGTGAATTACTGTGGCTGAGAAATCTGTTACAGGATTTAGGTTTCAAACTCAAAAGTACTATGCAGTTATATTGTGACAACCGGGCAGCCATTGACATATCACAGAATCCAGTACAGCATGATCGTACTAAGCATGTGGAGGTTGATCGTCATTTTATAAAAGAGAAGCTTGATGTCAAGCTTGTCAGCTTTCCTTTTGTCCCTTCTGAAGAGCAACTCGCAGATATTCTTACCAAATGA

Protein Analysis

871

Amino Acids

98.61

Weight (kDa)

8.0

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
rve PF00665 3 - 80 1.5e-08 Integrase core domain
SH3_retrovirus PF25597 158 - 219 3.1e-24 Retroviral polymerase SH3-like domain
RVT_2 PF07727 409 - 652 4.7e-75 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23160 AT4G23160
fragaria_vesca FvH4_2g08111 FvH4_2g17332 FvH4_2g18071 FvH4_2g20531 FvH4_2g20842 FvH4_2g20842 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g23401 FvH4_2g24231 FvH4_2g24232 FvH4_3g00831 FvH4_3g00831 FvH4_3g03561 FvH4_3g03561 FvH4_3g10282 FvH4_3g18165 FvH4_3g19021 FvH4_3g24071 FvH4_3g33123 FvH4_3g33970 FvH4_3g38851 FvH4_3g39133 FvH4_3g39271 FvH4_4g00981 FvH4_4g08191 FvH4_4g08311 FvH4_4g08311 FvH4_4g08311 FvH4_4g09341 FvH4_4g12550 FvH4_4g12550 FvH4_4g12550 FvH4_4g13391 FvH4_4g14842 FvH4_4g27901 FvH4_5g11561 FvH4_5g15611 FvH4_5g17991 FvH4_5g27871 FvH4_5g29995 FvH4_5g39662 FvH4_6g02392 FvH4_6g12781 FvH4_6g20106 FvH4_6g20106 FvH4_6g20106 FvH4_6g30872 FvH4_6g34901 FvH4_6g35632 FvH4_6g36661 FvH4_6g43795 FvH4_6g44611 FvH4_7g01791 FvH4_7g04041 FvH4_7g12551 FvH4_7g23351
malus_domestica MD00G1134200.v1.1 MD01G1096500.v1.1 MD01G1128200.v1.1 MD02G1250500.v1.1 MD02G1294900.v1.1 MD03G1121800.v1.1 MD04G1021400.v1.1 MD04G1051100.v1.1 MD04G1051300.v1.1 MD04G1099000.v1.1 MD05G1047900.v1.1 MD05G1298000.v1.1 MD06G1064200.v1.1 MD06G1120600.v1.1 MD07G1013900.v1.1 MD09G1226900.v1.1 MD10G1196700.v1.1 MD10G1307000.v1.1 MD10G1338900.v1.1 MD11G1107300.v1.1 MD11G1138300.v1.1 MD11G1236700.v1.1 MD12G1032500.v1.1 MD12G1150100.v1.1 MD12G1247800.v1.1 MD17G1267900.v1.1
pyrus_communis pycom02g03660 pycom02g13370 pycom02g14840 pycom02g18400 pycom02g18880 pycom02g22740 pycom04g08800 pycom04g15770 pycom05g07980 pycom05g19640 pycom06g09360 pycom07g13160 pycom08g11820 pycom08g21740 pycom09g19980 pycom10g04810 pycom10g05420 pycom10g14650 pycom11g03140 pycom11g21550 pycom12g14740 pycom13g01600 pycom13g29510 pycom14g07060 pycom15g01990 pycom15g19850 pycom15g30860 pycom16g15290 pycom16g16250 pycom16g25390 pycom17g17270 pycom520g00070
rosa_chinensis RchiOBHm_Chr1g0316691 RchiOBHm_Chr1g0320751 RchiOBHm_Chr1g0320771 RchiOBHm_Chr1g0322231 RchiOBHm_Chr1g0322861 RchiOBHm_Chr1g0328471 RchiOBHm_Chr1g0333321 RchiOBHm_Chr1g0337871 RchiOBHm_Chr1g0339051 RchiOBHm_Chr1g0349861 RchiOBHm_Chr1g0361951 RchiOBHm_Chr1g0365931 RchiOBHm_Chr1g0365981 RchiOBHm_Chr1g0377711 RchiOBHm_Chr1g0381581 RchiOBHm_Chr1g0382741 RchiOBHm_Chr1g0383501 RchiOBHm_Chr2g0101201 RchiOBHm_Chr2g0113601 RchiOBHm_Chr2g0124031 RchiOBHm_Chr2g0135481 RchiOBHm_Chr2g0148361 RchiOBHm_Chr2g0150101 RchiOBHm_Chr2g0154181 RchiOBHm_Chr2g0156071 RchiOBHm_Chr2g0166521 RchiOBHm_Chr2g0166821 RchiOBHm_Chr3g0448011 RchiOBHm_Chr3g0450941 RchiOBHm_Chr3g0450961 RchiOBHm_Chr3g0451051 RchiOBHm_Chr3g0451721 RchiOBHm_Chr3g0453371 RchiOBHm_Chr3g0454881 RchiOBHm_Chr3g0457421 RchiOBHm_Chr3g0457661 RchiOBHm_Chr3g0460011 RchiOBHm_Chr3g0461531 RchiOBHm_Chr3g0461981 RchiOBHm_Chr3g0463471 RchiOBHm_Chr3g0466641 RchiOBHm_Chr3g0468181 RchiOBHm_Chr3g0483351 RchiOBHm_Chr4g0394001 RchiOBHm_Chr4g0397261 RchiOBHm_Chr4g0404531 RchiOBHm_Chr4g0410841 RchiOBHm_Chr4g0415501 RchiOBHm_Chr4g0425221 RchiOBHm_Chr4g0430811 RchiOBHm_Chr5g0010831 RchiOBHm_Chr5g0016831 RchiOBHm_Chr5g0016851 RchiOBHm_Chr5g0031351 RchiOBHm_Chr5g0032151 RchiOBHm_Chr5g0041201 RchiOBHm_Chr5g0042191 RchiOBHm_Chr5g0064121 RchiOBHm_Chr5g0066601 RchiOBHm_Chr5g0078371 RchiOBHm_Chr5g0079271 RchiOBHm_Chr6g0263751 RchiOBHm_Chr6g0285111 RchiOBHm_Chr6g0298961 RchiOBHm_Chr6g0300481 RchiOBHm_Chr6g0303211 RchiOBHm_Chr7g0192341 RchiOBHm_Chr7g0196171 RchiOBHm_Chr7g0200281 RchiOBHm_Chr7g0200551 RchiOBHm_Chr7g0207611 RchiOBHm_Chr7g0212421 RchiOBHm_Chr7g0214991 RchiOBHm_Chr7g0219211 RchiOBHm_Chr7g0242371
rosa_laevigata RLG00000002389 RLG00000007980
rosa_roxburghii Rroxscaffold_1G00053480 Rroxscaffold_2G00090890 Rroxscaffold_2G00099660 Rroxscaffold_2G00134510 Rroxscaffold_3G00221450 Rroxscaffold_3G00240550 Rroxscaffold_4G00305710 Rroxscaffold_4G00306360 Rroxscaffold_4G00306800 Rroxscaffold_4G00329090 Rroxscaffold_5G00382750 Rroxscaffold_5G00385900 Rroxscaffold_6G00403080 Rroxscaffold_7G00172890 Rroxscaffold_7G00186690
rosa_samantha Rh1DG425600
rosa_wichuraiana Rw0G001640 Rw0G009160 Rw0G010480 Rw0G010520 Rw0G013850 Rw0G017170 Rw0G018520 Rw0G019280 Rw0G022780 Rw1G000360 Rw1G001410 Rw1G001630 Rw1G002100 Rw1G006810 Rw1G010680 Rw1G012700 Rw1G016160 Rw1G022180 Rw1G023580 Rw1G025640 Rw1G026310 Rw1G027230 Rw1G028110 Rw1G034180 Rw2G004990 Rw2G005840 Rw2G007350 Rw2G024730 Rw2G025580 Rw2G026670 Rw2G027210 Rw2G029060 Rw2G031560 Rw2G042190 Rw2G043850 Rw2G045990 Rw2G046130 Rw2G049290 Rw2G049310 Rw2G049790 Rw2G052250 Rw3G004350 Rw3G013100 Rw3G015280 Rw3G015720 Rw3G024630 Rw3G026470 Rw3G027840 Rw3G028960 Rw3G029130 Rw3G029160 Rw4G000280 Rw4G006650 Rw4G008970 Rw4G009310 Rw4G011730 Rw4G012000 Rw4G012610 Rw4G014300 Rw4G014690 Rw4G015190 Rw4G016470 Rw4G020750 Rw4G025060 Rw4G025080 Rw5G005610 Rw5G010380 Rw5G013970 Rw5G015130 Rw5G015250 Rw5G017640 Rw5G019910 Rw5G022410 Rw5G023550 Rw5G029710 Rw5G037650 Rw5G038280 Rw5G038570 Rw5G041550 Rw5G042600 Rw5G043910 Rw5G047850 Rw5G048200 Rw5G049920 Rw6G000260 Rw6G000530 Rw6G001710 Rw6G005090 Rw6G006730 Rw6G009310 Rw6G010950 Rw6G011630 Rw6G012080 Rw6G012750 Rw6G015970 Rw6G020700 Rw6G024350 Rw7G008540 Rw7G016620 Rw7G019400 Rw7G020010 Rw7G024240 Rw7G025630 Rw7G027550 Rw7G027770 Rw7G027960 Rw7G028450 Rw7G031280 Rw7G031970 Rw7G032180 Rw7G033590 Rw7G033720 Rw7G033890 Rw7G041800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2534
AasI GACNNNNNNGTC 1 cut(s) 872
AccB1I GGYRCC 1 cut(s) 832
AccBSI CCGCTC 1 cut(s) 665
AccI GTMKAC 2 cut(s) 1007, 1515
AccII CGCG 2 cut(s) 1611, 2332
AciI CCGC 3 cut(s) 555, 663, 764
AclWI GGATC 5 cut(s) 772, 1169, 1182, 1321, 2328
AcoI YGGCCR 1 cut(s) 1454
AcsI RAATTY 5 cut(s) 188, 399, 679, 793, 2136
AcuI CTGAAG 5 cut(s) 374, 653, 876, 882, 2604
AfiI CCNNNNNNNGG 1 cut(s) 2168
AflIII ACRYGT 1 cut(s) 1080
AgsI TTSAA 9 cut(s) 92, 113, 184, 692, 724, 843, 923, 2152, 2422
AjnI CCWGG 6 cut(s) 828, 1531, 1552, 1853, 2051, 2161
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 5 cut(s) 433, 1379, 1598, 1777, 1907
AlwI GGATC 5 cut(s) 772, 1169, 1182, 1321, 2328
AlwNI CAGNNNCTG 1 cut(s) 2225
Ama87I CYCGRG 3 cut(s) 522, 1011, 2104
AoxI GGCC 3 cut(s) 477, 1454, 2054
ApeKI GCWGC 3 cut(s) 1237, 1544, 2462
ApoI RAATTY 5 cut(s) 188, 399, 679, 793, 2136
ArsI GACNNNNNNTTYG 2 cut(s) 60, 92
Asp700I GAANNNNTTC 1 cut(s) 78
AspLEI GCGC 3 cut(s) 1613, 2031, 2332
AspS9I GGNCC 1 cut(s) 412
AsuC2I CCSGG 3 cut(s) 523, 524, 2459
AsuHPI GGTGA 6 cut(s) 206, 719, 939, 1242, 1568, 1596
AsuNHI GCTAGC 1 cut(s) 1789
AvaI CYCGRG 3 cut(s) 522, 1011, 2104
AvaII GGWCC 1 cut(s) 412
BaeGI GKGCMC 1 cut(s) 298
BalI TGGCCA 1 cut(s) 1456
BamHI GGATCC 1 cut(s) 1174
BanI GGYRCC 1 cut(s) 832
BarI GAAGNNNNNNTAC 2 cut(s) 1637, 1669
BauI CACGAG 1 cut(s) 291
BbsI GAAGAC 3 cut(s) 365, 870, 1517
Bbv12I GWGCWC 1 cut(s) 313
BbvCI CCTCAGC 2 cut(s) 1506, 2343
BbvI GCAGC 3 cut(s) 1249, 1531, 2474
BccI CCATC 7 cut(s) 385, 445, 591, 796, 1201, 1304, 1762
BciT130I CCWGG 6 cut(s) 830, 1533, 1554, 1855, 2053, 2163
BcnI CCSGG 3 cut(s) 523, 524, 2459
BcoDI GTCTC 5 cut(s) 433, 1379, 1598, 1777, 1907
BfaI CTAG 5 cut(s) 381, 416, 1067, 1089, 1790
BfmI CTRYAG 2 cut(s) 1269, 1778
BfoI RGCGCY 1 cut(s) 2032
BisI GCNGC 3 cut(s) 1238, 1545, 2463
BlsI GCNGC 3 cut(s) 1239, 1546, 2464
BmcAI AGTACT 3 cut(s) 602, 1832, 2434
Bme1390I CCNGG 9 cut(s) 523, 524, 830, 1533, 1554, 1855, 2053, 2163, 2459
Bme18I GGWCC 1 cut(s) 412
BmeT110I CYCGRG 3 cut(s) 522, 1011, 2104
BmgT120I GGNCC 1 cut(s) 412
BmiI GGNNCC 2 cut(s) 834, 1176
BmrFI CCNGG 9 cut(s) 523, 524, 830, 1533, 1554, 1855, 2053, 2163, 2459
BmsI GCATC 8 cut(s) 328, 428, 508, 834, 1121, 1153, 2113, 2212
BmtI GCTAGC 1 cut(s) 1793
BpiI GAAGAC 3 cut(s) 365, 870, 1517
BpmI CTGGAG 2 cut(s) 222, 2145
Bpu10I CCTNAGC 2 cut(s) 1506, 2343
BpuEI CTTGAG 1 cut(s) 2222
BpuMI CCSGG 3 cut(s) 523, 524, 2459
Bsa29I ATCGAT 1 cut(s) 1324
BsaI GGTCTC 1 cut(s) 1598
BsaJI CCNNGG 4 cut(s) 522, 1532, 1854, 2162
BsaXI ACNNNNNCTCC 4 cut(s) 153, 183, 1378, 1408
Bsc4I CCNNNNNNNGG 1 cut(s) 2168
Bse1I ACTGG 5 cut(s) 836, 958, 1753, 2337, 2486
Bse3DI GCAATG 2 cut(s) 1200, 1759
BseBI CCWGG 6 cut(s) 830, 1533, 1554, 1855, 2053, 2163
BseCI ATCGAT 1 cut(s) 1324
BseDI CCNNGG 4 cut(s) 522, 1532, 1854, 2162
BseGI GGATG 5 cut(s) 523, 583, 622, 825, 2128
BseLI CCNNNNNNNGG 1 cut(s) 2168
BseMI GCAATG 2 cut(s) 1200, 1759
BseMII CTCAG 8 cut(s) 927, 1373, 1497, 1683, 1788, 2334, 2340, 2384
BseNI ACTGG 5 cut(s) 836, 958, 1753, 2337, 2486
BseRI GAGGAG 3 cut(s) 343, 1523, 2184
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 3 cut(s) 1249, 1531, 2474
Bsh1236I CGCG 2 cut(s) 1611, 2332
BshFI GGCC 3 cut(s) 479, 1456, 2056
BshNI GGYRCC 1 cut(s) 832
BshVI ATCGAT 1 cut(s) 1324
BsiHKAI GWGCWC 1 cut(s) 313
BsiHKCI CYCGRG 3 cut(s) 522, 1011, 2104
BsiSI CCGG 2 cut(s) 523, 2458
BslFI GGGAC 2 cut(s) 860, 2561
BslI CCNNNNNNNGG 1 cut(s) 2168
BsmAI GTCTC 5 cut(s) 433, 1379, 1598, 1777, 1907
BsmFI GGGAC 2 cut(s) 860, 2561
BsmI GAATGC 2 cut(s) 1486, 2196
BsnI GGCC 3 cut(s) 479, 1456, 2056
Bso31I GGTCTC 1 cut(s) 1598
BsoBI CYCGRG 3 cut(s) 522, 1011, 2104
Bsp1286I GDGCHC 2 cut(s) 298, 313
Bsp1407I TGTACA 1 cut(s) 343
Bsp143I GATC 9 cut(s) 146, 777, 925, 1174, 1313, 1984, 2333, 2497, 2521
BspACI CCGC 3 cut(s) 555, 663, 764
BspANI GGCC 3 cut(s) 479, 1456, 2056
BspCNI CTCAG 8 cut(s) 928, 1372, 1498, 1682, 1789, 2335, 2341, 2385
BspDI ATCGAT 1 cut(s) 1324
BspFNI CGCG 2 cut(s) 1611, 2332
BspHI TCATGA 1 cut(s) 1638
BspLI GGNNCC 2 cut(s) 834, 1176
BspOI GCTAGC 1 cut(s) 1793
BspPI GGATC 5 cut(s) 772, 1169, 1182, 1321, 2328
BspQI GCTCTTC 3 cut(s) 60, 672, 2580
BspT107I GGYRCC 1 cut(s) 832
BspTNI GGTCTC 1 cut(s) 1598
BsrBI CCGCTC 1 cut(s) 665
BsrDI GCAATG 2 cut(s) 1200, 1759
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 5 cut(s) 836, 958, 1753, 2337, 2486
BssECI CCNNGG 4 cut(s) 522, 1532, 1854, 2162
BssMI GATC 9 cut(s) 146, 777, 925, 1174, 1313, 1984, 2333, 2497, 2521
BssNAI GTATAC 1 cut(s) 1516
BssSI CACGAG 1 cut(s) 291
Bst1107I GTATAC 1 cut(s) 1516
Bst2BI CACGAG 1 cut(s) 291
Bst2UI CCWGG 6 cut(s) 830, 1533, 1554, 1855, 2053, 2163
Bst6I CTCTTC 5 cut(s) 60, 672, 1197, 2300, 2580
BstAPI GCANNNNNTGC 1 cut(s) 1243
BstAUI TGTACA 1 cut(s) 343
BstC8I GCNNGC 3 cut(s) 1336, 1791, 2008
BstF5I GGATG 5 cut(s) 523, 583, 622, 825, 2128
BstFNI CGCG 2 cut(s) 1611, 2332
BstH2I RGCGCY 1 cut(s) 2032
BstHHI GCGC 3 cut(s) 1613, 2031, 2332
BstKTI GATC 9 cut(s) 149, 780, 928, 1177, 1316, 1987, 2336, 2500, 2524
BstMAI GTCTC 5 cut(s) 433, 1379, 1598, 1777, 1907
BstMBI GATC 9 cut(s) 146, 777, 925, 1174, 1313, 1984, 2333, 2497, 2521
BstMWI GCNNNNNNNGC 4 cut(s) 347, 1243, 1400, 2231
BstNI CCWGG 6 cut(s) 830, 1533, 1554, 1855, 2053, 2163
BstNSI RCATGY 3 cut(s) 228, 1084, 2513
BstSCI CCNGG 9 cut(s) 521, 522, 828, 1531, 1552, 1853, 2051, 2161, 2457
BstSFI CTRYAG 2 cut(s) 1269, 1778
BstSLI GKGCMC 1 cut(s) 298
BstUI CGCG 2 cut(s) 1611, 2332
BstV1I GCAGC 3 cut(s) 1249, 1531, 2474
BstV2I GAAGAC 3 cut(s) 365, 870, 1517
BstX2I RGATCY 2 cut(s) 777, 1174
BstYI RGATCY 2 cut(s) 777, 1174
BstZ17I GTATAC 1 cut(s) 1516
Bsu15I ATCGAT 1 cut(s) 1324
BsuRI GGCC 3 cut(s) 479, 1456, 2056
BsuTUI ATCGAT 1 cut(s) 1324
BtgZI GCGATG 1 cut(s) 351
BtsCI GGATG 5 cut(s) 523, 583, 622, 825, 2128
BtsIMutI CAGTG 6 cut(s) 846, 1041, 1290, 1323, 1746, 2344
Cac8I GCNNGC 3 cut(s) 1336, 1791, 2008
CaiI CAGNNNCTG 1 cut(s) 2225
CciI TCATGA 1 cut(s) 1638
CfoI GCGC 3 cut(s) 1613, 2031, 2332
Cfr13I GGNCC 1 cut(s) 412
Cfr9I CCCGGG 1 cut(s) 522
ClaI ATCGAT 1 cut(s) 1324
DpnI GATC 9 cut(s) 148, 779, 927, 1176, 1315, 1986, 2335, 2499, 2523
DpnII GATC 9 cut(s) 146, 777, 925, 1174, 1313, 1984, 2333, 2497, 2521
DraI TTTAAA 1 cut(s) 1449
DrdI GACNNNNNNGTC 1 cut(s) 872
DseDI GACNNNNNNGTC 1 cut(s) 872
EaeI YGGCCR 1 cut(s) 1454
Eam1104I CTCTTC 5 cut(s) 60, 672, 1197, 2300, 2580
EarI CTCTTC 5 cut(s) 60, 672, 1197, 2300, 2580
EciI GGCGGA 1 cut(s) 570
Eco31I GGTCTC 1 cut(s) 1598
Eco32I GATATC 1 cut(s) 560
Eco47I GGWCC 1 cut(s) 412
Eco57I CTGAAG 5 cut(s) 374, 653, 876, 882, 2604
Eco88I CYCGRG 3 cut(s) 522, 1011, 2104
EcoO109I RGGNCCY 1 cut(s) 412
EcoRI GAATTC 1 cut(s) 679
EcoRII CCWGG 6 cut(s) 828, 1531, 1552, 1853, 2051, 2161
EcoRV GATATC 1 cut(s) 560
EcoT22I ATGCAT 3 cut(s) 1114, 1486, 2100
FalI AAGNNNNNCTT 6 cut(s) 1323, 1355, 1820, 1852, 2529, 2561
FaqI GGGAC 2 cut(s) 860, 2561
FauI CCCGC 1 cut(s) 757
FauNDI CATATG 4 cut(s) 315, 1108, 1366, 2068
FblI GTMKAC 2 cut(s) 1007, 1515
Fnu4HI GCNGC 3 cut(s) 1238, 1545, 2463
FokI GGATG 5 cut(s) 530, 570, 629, 812, 2135
Fsp4HI GCNGC 3 cut(s) 1238, 1545, 2463
FspBI CTAG 5 cut(s) 381, 416, 1067, 1089, 1790
GlaI GCGC 3 cut(s) 1612, 2030, 2331
GluI GCNGC 3 cut(s) 1238, 1545, 2463
GsuI CTGGAG 2 cut(s) 222, 2145
HaeII RGCGCY 1 cut(s) 2032
HaeIII GGCC 3 cut(s) 479, 1456, 2056
HapII CCGG 2 cut(s) 523, 2458
HhaI GCGC 3 cut(s) 1613, 2031, 2332
Hin6I GCGC 3 cut(s) 1611, 2029, 2330
HinP1I GCGC 3 cut(s) 1611, 2029, 2330
HincII GTYRAC 2 cut(s) 1019, 2259
HindII GTYRAC 2 cut(s) 1019, 2259
HindIII AAGCTT 2 cut(s) 2543, 2555
HinfI GANTC 8 cut(s) 138, 235, 971, 1123, 1321, 1522, 2113, 2482
HpaII CCGG 2 cut(s) 523, 2458
HphI GGTGA 6 cut(s) 206, 719, 939, 1242, 1568, 1596
Hpy166II GTNNAC 8 cut(s) 947, 1008, 1019, 1086, 1290, 1356, 1516, 2259
Hpy188III TCNNGA 7 cut(s) 239, 1477, 1639, 1804, 1898, 1982, 2201
Hpy8I GTNNAC 8 cut(s) 947, 1008, 1019, 1086, 1290, 1356, 1516, 2259
HpyF10VI GCNNNNNNNGC 4 cut(s) 347, 1243, 1400, 2231
HspAI GCGC 3 cut(s) 1611, 2029, 2330
Kzo9I GATC 9 cut(s) 146, 777, 925, 1174, 1313, 1984, 2333, 2497, 2521
LguI GCTCTTC 3 cut(s) 60, 672, 2580
LmnI GCTCC 4 cut(s) 823, 895, 1399, 2164
Lsp1109I GCAGC 3 cut(s) 1249, 1531, 2474
LweI GCATC 8 cut(s) 328, 428, 508, 834, 1121, 1153, 2113, 2212
MaeI CTAG 5 cut(s) 381, 416, 1067, 1089, 1790
MalI GATC 9 cut(s) 148, 779, 927, 1176, 1315, 1986, 2335, 2499, 2523
MbiI CCGCTC 1 cut(s) 665
MboI GATC 9 cut(s) 146, 777, 925, 1174, 1313, 1984, 2333, 2497, 2521
MflI RGATCY 2 cut(s) 777, 1174
MhlI GDGCHC 2 cut(s) 298, 313
MlsI TGGCCA 1 cut(s) 1456
MluNI TGGCCA 1 cut(s) 1456
MlyI GAGTC 5 cut(s) 147, 229, 980, 1531, 2122
MmeI TCCRAC 3 cut(s) 495, 1486, 1905
Mox20I TGGCCA 1 cut(s) 1456
Mph1103I ATGCAT 3 cut(s) 1114, 1486, 2100
MroXI GAANNNNTTC 1 cut(s) 78
MscI TGGCCA 1 cut(s) 1456
MseI TTAA 5 cut(s) 171, 531, 1448, 1574, 2141
MslI CAYNNNNRTG 1 cut(s) 2121
Msp20I TGGCCA 1 cut(s) 1456
MspA1I CMGCKG 1 cut(s) 764
MspI CCGG 2 cut(s) 523, 2458
MspR9I CCNGG 9 cut(s) 523, 524, 830, 1533, 1554, 1855, 2053, 2163, 2459
Mva1269I GAATGC 2 cut(s) 1486, 2196
MvaI CCWGG 6 cut(s) 830, 1533, 1554, 1855, 2053, 2163
MvnI CGCG 2 cut(s) 1611, 2332
MwoI GCNNNNNNNGC 4 cut(s) 347, 1243, 1400, 2231
NciI CCSGG 3 cut(s) 523, 524, 2459
NdeI CATATG 4 cut(s) 315, 1108, 1366, 2068
NdeII GATC 9 cut(s) 146, 777, 925, 1174, 1313, 1984, 2333, 2497, 2521
NheI GCTAGC 1 cut(s) 1789
NlaIV GGNNCC 2 cut(s) 834, 1176
NmuCI GTSAC 3 cut(s) 1602, 2114, 2450
NsiI ATGCAT 3 cut(s) 1114, 1486, 2100
NspI RCATGY 3 cut(s) 228, 1084, 2513
PaeR7I CTCGAG 1 cut(s) 1011
PagI TCATGA 1 cut(s) 1638
PciI ACATGT 1 cut(s) 1080
PciSI GCTCTTC 3 cut(s) 60, 672, 2580
PcsI WCGNNNNNNNCGW 2 cut(s) 142, 268
PctI GAATGC 2 cut(s) 1486, 2196
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 3 cut(s) 1123, 1321, 2482
PkrI GCNGC 3 cut(s) 1239, 1546, 2464
PleI GAGTC 5 cut(s) 146, 229, 979, 1530, 2121
PpsI GAGTC 5 cut(s) 146, 229, 979, 1530, 2121
PpuMI RGGWCCY 1 cut(s) 412
PscI ACATGT 1 cut(s) 1080
PsiI TTATAA 1 cut(s) 2534
Psp5II RGGWCCY 1 cut(s) 412
Psp6I CCWGG 6 cut(s) 828, 1531, 1552, 1853, 2051, 2161
PspGI CCWGG 6 cut(s) 828, 1531, 1552, 1853, 2051, 2161
PspN4I GGNNCC 2 cut(s) 834, 1176
PspPI GGNCC 1 cut(s) 412
PspPPI RGGWCCY 1 cut(s) 412
PspXI VCTCGAGB 1 cut(s) 1011
PstNI CAGNNNCTG 1 cut(s) 2225
PsuI RGATCY 2 cut(s) 777, 1174
RseI CAYNNNNRTG 1 cut(s) 2121
SapI GCTCTTC 3 cut(s) 60, 672, 2580
SaqAI TTAA 5 cut(s) 171, 531, 1448, 1574, 2141
SatI GCNGC 3 cut(s) 1238, 1545, 2463
Sau3AI GATC 9 cut(s) 146, 777, 925, 1174, 1313, 1984, 2333, 2497, 2521
Sau96I GGNCC 1 cut(s) 412
ScaI AGTACT 3 cut(s) 602, 1832, 2434
SchI GAGTC 5 cut(s) 147, 229, 980, 1531, 2122
ScrFI CCNGG 9 cut(s) 523, 524, 830, 1533, 1554, 1855, 2053, 2163, 2459
SduI GDGCHC 2 cut(s) 298, 313
SfaNI GCATC 8 cut(s) 328, 428, 508, 834, 1121, 1153, 2113, 2212
SfcI CTRYAG 2 cut(s) 1269, 1778
Sfr274I CTCGAG 1 cut(s) 1011
SinI GGWCC 1 cut(s) 412
SlaI CTCGAG 1 cut(s) 1011
SmaI CCCGGG 1 cut(s) 524
SmiI ATTTAAAT 1 cut(s) 1449
SmiMI CAYNNNNRTG 1 cut(s) 2121
SmlI CTYRAG 2 cut(s) 1011, 2201
SmoI CTYRAG 2 cut(s) 1011, 2201
SrfI GCCCGGGC 1 cut(s) 524
SsiI CCGC 3 cut(s) 555, 663, 764
SspMI CTAG 5 cut(s) 381, 416, 1067, 1089, 1790
StyD4I CCNGG 9 cut(s) 521, 522, 828, 1531, 1552, 1853, 2051, 2161, 2457
SwaI ATTTAAAT 1 cut(s) 1449
TaqI TCGA 4 cut(s) 136, 1012, 1324, 1803
TatI WGTACW 6 cut(s) 343, 600, 1156, 1830, 2432, 2488
TfiI GAWTC 3 cut(s) 1123, 1321, 2482
Tru1I TTAA 5 cut(s) 171, 531, 1448, 1574, 2141
Tru9I TTAA 5 cut(s) 171, 531, 1448, 1574, 2141
TscAI CASTG 6 cut(s) 853, 1048, 1297, 1323, 1753, 2344
TseFI GTSAC 3 cut(s) 1602, 2114, 2450
TseI GCWGC 3 cut(s) 1237, 1544, 2462
Tsp45I GTSAC 3 cut(s) 1602, 2114, 2450
TspGWI ACGGA 1 cut(s) 277
TspMI CCCGGG 1 cut(s) 522
TspRI CASTG 6 cut(s) 853, 1048, 1297, 1323, 1753, 2344
VpaK11BI GGWCC 1 cut(s) 412
XapI RAATTY 5 cut(s) 188, 399, 679, 793, 2136
XceI RCATGY 3 cut(s) 228, 1084, 2513
XhoI CTCGAG 1 cut(s) 1011
XmaI CCCGGG 1 cut(s) 522
XmiI GTMKAC 2 cut(s) 1007, 1515
XmnI GAANNNNTTC 1 cut(s) 78
XspI CTAG 5 cut(s) 381, 416, 1067, 1089, 1790
ZrmI AGTACT 3 cut(s) 602, 1832, 2434
Zsp2I ATGCAT 3 cut(s) 1114, 1486, 2100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.