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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 721 |
| AccI | GTMKAC | 1 cut(s) | 29 |
| AccII | CGCG | 1 cut(s) | 42 |
| AciI | CCGC | 3 cut(s) | 7, 245, 473 |
| AclWI | GGATC | 2 cut(s) | 749, 762 |
| AcoI | YGGCCR | 1 cut(s) | 202 |
| AcuI | CTGAAG | 1 cut(s) | 542 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 285 |
| AgsI | TTSAA | 2 cut(s) | 136, 469 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 301 |
| AjnI | CCWGG | 2 cut(s) | 48, 199 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 128, 160 |
| AluBI | AGCT | 5 cut(s) | 114, 432, 463, 520, 845 |
| AluI | AGCT | 5 cut(s) | 114, 432, 463, 520, 845 |
| Alw21I | GWGCWC | 1 cut(s) | 465 |
| Alw26I | GTCTC | 1 cut(s) | 758 |
| AlwI | GGATC | 2 cut(s) | 749, 762 |
| AoxI | GGCC | 3 cut(s) | 202, 655, 833 |
| ApaI | GGGCCC | 1 cut(s) | 659 |
| ApeKI | GCWGC | 1 cut(s) | 37 |
| AspLEI | GCGC | 2 cut(s) | 42, 100 |
| AspS9I | GGNCC | 5 cut(s) | 266, 276, 655, 656, 833 |
| AsuHPI | GGTGA | 2 cut(s) | 607, 709 |
| AsuII | TTCGAA | 1 cut(s) | 641 |
| AsuNHI | GCTAGC | 1 cut(s) | 236 |
| AvaII | GGWCC | 2 cut(s) | 266, 276 |
| AxyI | CCTNAGG | 1 cut(s) | 342 |
| BaeGI | GKGCMC | 1 cut(s) | 659 |
| BalI | TGGCCA | 1 cut(s) | 204 |
| BamHI | GGATCC | 1 cut(s) | 754 |
| BanI | GGYRCC | 1 cut(s) | 721 |
| BanII | GRGCYC | 2 cut(s) | 465, 659 |
| Bbv12I | GWGCWC | 1 cut(s) | 465 |
| BbvI | GCAGC | 1 cut(s) | 49 |
| BccI | CCATC | 4 cut(s) | 410, 477, 745, 823 |
| BceAI | ACGGC | 1 cut(s) | 380 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 44, 78 |
| BciT130I | CCWGG | 2 cut(s) | 50, 201 |
| BcoDI | GTCTC | 1 cut(s) | 758 |
| BfaI | CTAG | 2 cut(s) | 237, 660 |
| BfmI | CTRYAG | 2 cut(s) | 35, 126 |
| BfoI | RGCGCY | 1 cut(s) | 101 |
| BglII | AGATCT | 1 cut(s) | 207 |
| BisI | GCNGC | 1 cut(s) | 38 |
| BlpI | GCTNAGC | 1 cut(s) | 516 |
| BlsI | GCNGC | 1 cut(s) | 39 |
| Bme1390I | CCNGG | 2 cut(s) | 50, 201 |
| Bme18I | GGWCC | 2 cut(s) | 266, 276 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 301 |
| BmgT120I | GGNCC | 5 cut(s) | 266, 276, 655, 656, 833 |
| BmiI | GGNNCC | 7 cut(s) | 277, 333, 367, 657, 723, 756, 834 |
| BmrFI | CCNGG | 2 cut(s) | 50, 201 |
| BmsI | GCATC | 2 cut(s) | 97, 550 |
| BmtI | GCTAGC | 1 cut(s) | 240 |
| BpmI | CTGGAG | 1 cut(s) | 71 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 516 |
| Bpu14I | TTCGAA | 1 cut(s) | 641 |
| BpuEI | CTTGAG | 1 cut(s) | 713 |
| BsaJI | CCNNGG | 2 cut(s) | 199, 456 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 285 |
| Bse118I | RCCGGY | 1 cut(s) | 268 |
| Bse1I | ACTGG | 1 cut(s) | 725 |
| Bse21I | CCTNAGG | 1 cut(s) | 342 |
| Bse3DI | GCAATG | 1 cut(s) | 247 |
| BseBI | CCWGG | 2 cut(s) | 50, 201 |
| BseDI | CCNNGG | 2 cut(s) | 199, 456 |
| BseGI | GGATG | 1 cut(s) | 739 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 285 |
| BseMI | GCAATG | 1 cut(s) | 247 |
| BseMII | CTCAG | 1 cut(s) | 470 |
| BseNI | ACTGG | 1 cut(s) | 725 |
| BseSI | GKGCMC | 1 cut(s) | 659 |
| BseXI | GCAGC | 1 cut(s) | 49 |
| BseYI | CCCAGC | 1 cut(s) | 272 |
| Bsh1236I | CGCG | 1 cut(s) | 42 |
| BshFI | GGCC | 3 cut(s) | 204, 657, 835 |
| BshNI | GGYRCC | 1 cut(s) | 721 |
| BsiHKAI | GWGCWC | 1 cut(s) | 465 |
| BsiSI | CCGG | 1 cut(s) | 269 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 285 |
| BsmAI | GTCTC | 1 cut(s) | 758 |
| BsmI | GAATGC | 2 cut(s) | 324, 493 |
| BsnI | GGCC | 3 cut(s) | 204, 657, 835 |
| Bsp119I | TTCGAA | 1 cut(s) | 641 |
| Bsp120I | GGGCCC | 1 cut(s) | 655 |
| Bsp1286I | GDGCHC | 2 cut(s) | 465, 659 |
| Bsp143I | GATC | 2 cut(s) | 207, 754 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 516 |
| BspACI | CCGC | 3 cut(s) | 7, 245, 473 |
| BspANI | GGCC | 3 cut(s) | 204, 657, 835 |
| BspCNI | CTCAG | 1 cut(s) | 471 |
| BspFNI | CGCG | 1 cut(s) | 42 |
| BspHI | TCATGA | 1 cut(s) | 847 |
| BspLI | GGNNCC | 7 cut(s) | 277, 333, 367, 657, 723, 756, 834 |
| BspMAI | CTGCAG | 2 cut(s) | 39, 130 |
| BspOI | GCTAGC | 1 cut(s) | 240 |
| BspPI | GGATC | 2 cut(s) | 749, 762 |
| BspT104I | TTCGAA | 1 cut(s) | 641 |
| BspT107I | GGYRCC | 1 cut(s) | 721 |
| BsrDI | GCAATG | 1 cut(s) | 247 |
| BsrFI | RCCGGY | 1 cut(s) | 268 |
| BsrI | ACTGG | 1 cut(s) | 725 |
| BssAI | RCCGGY | 1 cut(s) | 268 |
| BssECI | CCNNGG | 2 cut(s) | 199, 456 |
| BssMI | GATC | 2 cut(s) | 207, 754 |
| BssNAI | GTATAC | 1 cut(s) | 30 |
| BssT1I | CCWWGG | 1 cut(s) | 456 |
| Bst1107I | GTATAC | 1 cut(s) | 30 |
| Bst2UI | CCWGG | 2 cut(s) | 50, 201 |
| Bst4CI | ACNGT | 1 cut(s) | 299 |
| Bst6I | CTCTTC | 2 cut(s) | 158, 286 |
| BstBI | TTCGAA | 1 cut(s) | 641 |
| BstC8I | GCNNGC | 2 cut(s) | 86, 238 |
| BstDEI | CTNAG | 3 cut(s) | 342, 479, 516 |
| BstF5I | GGATG | 1 cut(s) | 739 |
| BstFNI | CGCG | 1 cut(s) | 42 |
| BstH2I | RGCGCY | 1 cut(s) | 101 |
| BstHHI | GCGC | 2 cut(s) | 42, 100 |
| BstKTI | GATC | 2 cut(s) | 210, 757 |
| BstMAI | GTCTC | 1 cut(s) | 758 |
| BstMBI | GATC | 2 cut(s) | 207, 754 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 111, 210, 242, 588 |
| BstNI | CCWGG | 2 cut(s) | 50, 201 |
| BstSCI | CCNGG | 2 cut(s) | 48, 199 |
| BstSFI | CTRYAG | 2 cut(s) | 35, 126 |
| BstSLI | GKGCMC | 1 cut(s) | 659 |
| BstUI | CGCG | 1 cut(s) | 42 |
| BstV1I | GCAGC | 1 cut(s) | 49 |
| BstX2I | RGATCY | 2 cut(s) | 207, 754 |
| BstYI | RGATCY | 2 cut(s) | 207, 754 |
| BstZ17I | GTATAC | 1 cut(s) | 30 |
| Bsu36I | CCTNAGG | 1 cut(s) | 342 |
| BsuRI | GGCC | 3 cut(s) | 204, 657, 835 |
| BtsCI | GGATG | 1 cut(s) | 739 |
| BtsI | GCAGTG | 2 cut(s) | 32, 135 |
| BtsIMutI | CAGTG | 2 cut(s) | 32, 135 |
| Cac8I | GCNNGC | 2 cut(s) | 86, 238 |
| CciI | TCATGA | 1 cut(s) | 847 |
| CfoI | GCGC | 2 cut(s) | 42, 100 |
| Cfr10I | RCCGGY | 1 cut(s) | 268 |
| Cfr13I | GGNCC | 5 cut(s) | 266, 276, 655, 656, 833 |
| CviAII | CATG | 2 cut(s) | 592, 848 |
| DdeI | CTNAG | 3 cut(s) | 342, 479, 516 |
| DpnI | GATC | 2 cut(s) | 209, 756 |
| DpnII | GATC | 2 cut(s) | 207, 754 |
| DriI | GACNNNNNGTC | 1 cut(s) | 301 |
| EaeI | YGGCCR | 1 cut(s) | 202 |
| Eam1104I | CTCTTC | 2 cut(s) | 158, 286 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 301 |
| EarI | CTCTTC | 2 cut(s) | 158, 286 |
| Ecl136II | GAGCTC | 1 cut(s) | 463 |
| Eco130I | CCWWGG | 1 cut(s) | 456 |
| Eco24I | GRGCYC | 2 cut(s) | 465, 659 |
| Eco47I | GGWCC | 2 cut(s) | 266, 276 |
| Eco53kI | GAGCTC | 1 cut(s) | 463 |
| Eco57I | CTGAAG | 1 cut(s) | 542 |
| Eco81I | CCTNAGG | 1 cut(s) | 342 |
| EcoICRI | GAGCTC | 1 cut(s) | 463 |
| EcoO109I | RGGNCCY | 4 cut(s) | 276, 655, 656, 833 |
| EcoRII | CCWGG | 2 cut(s) | 48, 199 |
| EcoT14I | CCWWGG | 1 cut(s) | 456 |
| EcoT22I | ATGCAT | 1 cut(s) | 107 |
| EcoT38I | GRGCYC | 2 cut(s) | 465, 659 |
| ErhI | CCWWGG | 1 cut(s) | 456 |
| FaeI | CATG | 2 cut(s) | 595, 851 |
| FaiI | YATR | 9 cut(s) | 12, 30, 94, 103, 153, 495, 572, 593, 849 |
| FatI | CATG | 2 cut(s) | 591, 847 |
| FblI | GTMKAC | 1 cut(s) | 29 |
| Fnu4HI | GCNGC | 1 cut(s) | 38 |
| FokI | GGATG | 1 cut(s) | 746 |
| FriOI | GRGCYC | 2 cut(s) | 465, 659 |
| Fsp4HI | GCNGC | 1 cut(s) | 38 |
| FspBI | CTAG | 2 cut(s) | 237, 660 |
| GlaI | GCGC | 2 cut(s) | 41, 99 |
| GluI | GCNGC | 1 cut(s) | 38 |
| GsaI | CCCAGC | 1 cut(s) | 276 |
| GsuI | CTGGAG | 1 cut(s) | 71 |
| HaeII | RGCGCY | 1 cut(s) | 101 |
| HaeIII | GGCC | 3 cut(s) | 204, 657, 835 |
| HapII | CCGG | 1 cut(s) | 269 |
| HhaI | GCGC | 2 cut(s) | 42, 100 |
| Hin1II | CATG | 2 cut(s) | 595, 851 |
| Hin6I | GCGC | 2 cut(s) | 40, 98 |
| HinP1I | GCGC | 2 cut(s) | 40, 98 |
| HincII | GTYRAC | 1 cut(s) | 384 |
| HindII | GTYRAC | 1 cut(s) | 384 |
| HindIII | AAGCTT | 1 cut(s) | 430 |
| HinfI | GANTC | 1 cut(s) | 530 |
| HpaI | GTTAAC | 1 cut(s) | 384 |
| HpaII | CCGG | 1 cut(s) | 269 |
| HphI | GGTGA | 2 cut(s) | 607, 709 |
| Hpy166II | GTNNAC | 3 cut(s) | 30, 384, 448 |
| Hpy188III | TCNNGA | 4 cut(s) | 264, 466, 673, 848 |
| Hpy8I | GTNNAC | 3 cut(s) | 30, 384, 448 |
| HpyAV | CCTTC | 7 cut(s) | 130, 226, 463, 505, 517, 646, 698 |
| HpyCH4III | ACNGT | 1 cut(s) | 299 |
| HpyCH4IV | ACGT | 5 cut(s) | 350, 386, 407, 497, 622 |
| HpyCH4V | TGCA | 5 cut(s) | 37, 105, 128, 359, 491 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 111, 210, 242, 588 |
| HpyF3I | CTNAG | 3 cut(s) | 342, 479, 516 |
| HpySE526I | ACGT | 5 cut(s) | 350, 386, 407, 497, 622 |
| Hsp92II | CATG | 2 cut(s) | 595, 851 |
| HspAI | GCGC | 2 cut(s) | 40, 98 |
| KspAI | GTTAAC | 1 cut(s) | 384 |
| Kzo9I | GATC | 2 cut(s) | 207, 754 |
| LmnI | GCTCC | 3 cut(s) | 52, 337, 460 |
| Lsp1109I | GCAGC | 1 cut(s) | 49 |
| LweI | GCATC | 2 cut(s) | 97, 550 |
| MaeI | CTAG | 2 cut(s) | 237, 660 |
| MaeII | ACGT | 5 cut(s) | 350, 386, 407, 497, 622 |
| MaeIII | GTNAC | 1 cut(s) | 709 |
| MalI | GATC | 2 cut(s) | 209, 756 |
| MboI | GATC | 2 cut(s) | 207, 754 |
| MboII | GAAGA | 3 cut(s) | 175, 273, 625 |
| MflI | RGATCY | 2 cut(s) | 207, 754 |
| MhlI | GDGCHC | 2 cut(s) | 465, 659 |
| MlsI | TGGCCA | 1 cut(s) | 204 |
| MluCI | AATT | 2 cut(s) | 218, 539 |
| MluNI | TGGCCA | 1 cut(s) | 204 |
| Mox20I | TGGCCA | 1 cut(s) | 204 |
| Mph1103I | ATGCAT | 1 cut(s) | 107 |
| MscI | TGGCCA | 1 cut(s) | 204 |
| MseI | TTAA | 1 cut(s) | 383 |
| Msp20I | TGGCCA | 1 cut(s) | 204 |
| MspI | CCGG | 1 cut(s) | 269 |
| MspR9I | CCNGG | 2 cut(s) | 50, 201 |
| Mva1269I | GAATGC | 2 cut(s) | 324, 493 |
| MvaI | CCWGG | 2 cut(s) | 50, 201 |
| MvnI | CGCG | 1 cut(s) | 42 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 111, 210, 242, 588 |
| NdeII | GATC | 2 cut(s) | 207, 754 |
| NheI | GCTAGC | 1 cut(s) | 236 |
| NlaIII | CATG | 2 cut(s) | 595, 851 |
| NlaIV | GGNNCC | 7 cut(s) | 277, 333, 367, 657, 723, 756, 834 |
| NsiI | ATGCAT | 1 cut(s) | 107 |
| NspV | TTCGAA | 1 cut(s) | 641 |
| PagI | TCATGA | 1 cut(s) | 847 |
| PctI | GAATGC | 2 cut(s) | 324, 493 |
| PfeI | GAWTC | 1 cut(s) | 530 |
| PfoI | TCCNGGA | 1 cut(s) | 48 |
| PkrI | GCNGC | 1 cut(s) | 39 |
| PpuMI | RGGWCCY | 1 cut(s) | 276 |
| Psp124BI | GAGCTC | 1 cut(s) | 465 |
| Psp5II | RGGWCCY | 1 cut(s) | 276 |
| Psp6I | CCWGG | 2 cut(s) | 48, 199 |
| PspFI | CCCAGC | 1 cut(s) | 272 |
| PspGI | CCWGG | 2 cut(s) | 48, 199 |
| PspN4I | GGNNCC | 7 cut(s) | 277, 333, 367, 657, 723, 756, 834 |
| PspOMI | GGGCCC | 1 cut(s) | 655 |
| PspPI | GGNCC | 5 cut(s) | 266, 276, 655, 656, 833 |
| PspPPI | RGGWCCY | 1 cut(s) | 276 |
| PstI | CTGCAG | 2 cut(s) | 39, 130 |
| PsuI | RGATCY | 2 cut(s) | 207, 754 |
| SacI | GAGCTC | 1 cut(s) | 465 |
| SaqAI | TTAA | 1 cut(s) | 383 |
| SatI | GCNGC | 1 cut(s) | 38 |
| Sau3AI | GATC | 2 cut(s) | 207, 754 |
| Sau96I | GGNCC | 5 cut(s) | 266, 276, 655, 656, 833 |
| ScrFI | CCNGG | 2 cut(s) | 50, 201 |
| SduI | GDGCHC | 2 cut(s) | 465, 659 |
| SfaNI | GCATC | 2 cut(s) | 97, 550 |
| SfcI | CTRYAG | 2 cut(s) | 35, 126 |
| SfuI | TTCGAA | 1 cut(s) | 641 |
| SinI | GGWCC | 2 cut(s) | 266, 276 |
| SmlI | CTYRAG | 1 cut(s) | 692 |
| SmoI | CTYRAG | 1 cut(s) | 692 |
| Sse9I | AATT | 2 cut(s) | 218, 539 |
| SsiI | CCGC | 3 cut(s) | 7, 245, 473 |
| SspMI | CTAG | 2 cut(s) | 237, 660 |
| SstI | GAGCTC | 1 cut(s) | 465 |
| StyD4I | CCNGG | 2 cut(s) | 48, 199 |
| StyI | CCWWGG | 1 cut(s) | 456 |
| TaaI | ACNGT | 1 cut(s) | 299 |
| TaiI | ACGT | 5 cut(s) | 353, 389, 410, 500, 625 |
| TaqI | TCGA | 3 cut(s) | 15, 305, 641 |
| TasI | AATT | 2 cut(s) | 218, 539 |
| TfiI | GAWTC | 1 cut(s) | 530 |
| Tru1I | TTAA | 1 cut(s) | 383 |
| Tru9I | TTAA | 1 cut(s) | 383 |
| TscAI | CASTG | 2 cut(s) | 39, 135 |
| TseI | GCWGC | 1 cut(s) | 37 |
| TspDTI | ATGAA | 1 cut(s) | 559 |
| TspGWI | ACGGA | 1 cut(s) | 308 |
| TspRI | CASTG | 2 cut(s) | 39, 135 |
| VpaK11BI | GGWCC | 2 cut(s) | 266, 276 |
| XmiI | GTMKAC | 1 cut(s) | 29 |
| XspI | CTAG | 2 cut(s) | 237, 660 |
| Zsp2I | ATGCAT | 1 cut(s) | 107 |