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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 8 |
| AccI | GTMKAC | 1 cut(s) | 417 |
| AciI | CCGC | 2 cut(s) | 89, 269 |
| AclWI | GGATC | 1 cut(s) | 367 |
| AcoI | YGGCCR | 1 cut(s) | 129 |
| AcyI | GRCGYC | 1 cut(s) | 5 |
| AdeI | CACNNNGTG | 1 cut(s) | 452 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 217 |
| AflIII | ACRYGT | 1 cut(s) | 33 |
| AgsI | TTSAA | 2 cut(s) | 253, 328 |
| AjnI | CCWGG | 1 cut(s) | 112 |
| AluBI | AGCT | 3 cut(s) | 107, 170, 279 |
| AluI | AGCT | 3 cut(s) | 107, 170, 279 |
| Alw26I | GTCTC | 2 cut(s) | 12, 297 |
| AlwI | GGATC | 1 cut(s) | 367 |
| AoxI | GGCC | 3 cut(s) | 110, 129, 310 |
| AspS9I | GGNCC | 2 cut(s) | 110, 310 |
| AsuHPI | GGTGA | 3 cut(s) | 316, 464, 473 |
| AsuNHI | GCTAGC | 1 cut(s) | 103 |
| AxyI | CCTNAGG | 3 cut(s) | 183, 243, 300 |
| BalI | TGGCCA | 1 cut(s) | 131 |
| BccI | CCATC | 1 cut(s) | 140 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 82, 116 |
| BciT130I | CCWGG | 1 cut(s) | 114 |
| BciVI | GTATCC | 1 cut(s) | 391 |
| BcoDI | GTCTC | 2 cut(s) | 12, 297 |
| BfaI | CTAG | 2 cut(s) | 104, 351 |
| BfuI | GTATCC | 1 cut(s) | 391 |
| BisI | GCNGC | 1 cut(s) | 90 |
| BlsI | GCNGC | 1 cut(s) | 91 |
| Bme1390I | CCNGG | 1 cut(s) | 114 |
| BmgT120I | GGNCC | 2 cut(s) | 110, 310 |
| BmiI | GGNNCC | 1 cut(s) | 369 |
| BmrFI | CCNGG | 1 cut(s) | 114 |
| BmtI | GCTAGC | 1 cut(s) | 107 |
| BsaHI | GRCGYC | 1 cut(s) | 5 |
| BsaJI | CCNNGG | 1 cut(s) | 113 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 217 |
| Bse1I | ACTGG | 1 cut(s) | 132 |
| Bse21I | CCTNAGG | 3 cut(s) | 183, 243, 300 |
| BseBI | CCWGG | 1 cut(s) | 114 |
| BseDI | CCNNGG | 1 cut(s) | 113 |
| BseGI | GGATG | 2 cut(s) | 132, 238 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 217 |
| BseMII | CTCAG | 1 cut(s) | 197 |
| BseNI | ACTGG | 1 cut(s) | 132 |
| BseRI | GAGGAG | 1 cut(s) | 359 |
| BseYI | CCCAGC | 1 cut(s) | 107 |
| BshFI | GGCC | 3 cut(s) | 112, 131, 312 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 217 |
| BsmAI | GTCTC | 2 cut(s) | 12, 297 |
| BsmBI | CGTCTC | 1 cut(s) | 12 |
| BsnI | GGCC | 3 cut(s) | 112, 131, 312 |
| Bsp143I | GATC | 1 cut(s) | 372 |
| BspACI | CCGC | 2 cut(s) | 89, 269 |
| BspANI | GGCC | 3 cut(s) | 112, 131, 312 |
| BspCNI | CTCAG | 1 cut(s) | 196 |
| BspLI | GGNNCC | 1 cut(s) | 369 |
| BspOI | GCTAGC | 1 cut(s) | 107 |
| BspPI | GGATC | 1 cut(s) | 367 |
| BsrI | ACTGG | 1 cut(s) | 132 |
| BssECI | CCNNGG | 1 cut(s) | 113 |
| BssMI | GATC | 1 cut(s) | 372 |
| BssNI | GRCGYC | 1 cut(s) | 5 |
| Bst2UI | CCWGG | 1 cut(s) | 114 |
| Bst4CI | ACNGT | 2 cut(s) | 403, 411 |
| Bst6I | CTCTTC | 1 cut(s) | 311 |
| BstACI | GRCGYC | 1 cut(s) | 5 |
| BstC8I | GCNNGC | 2 cut(s) | 105, 168 |
| BstDEI | CTNAG | 4 cut(s) | 50, 183, 243, 300 |
| BstF5I | GGATG | 2 cut(s) | 132, 238 |
| BstKTI | GATC | 1 cut(s) | 375 |
| BstMAI | GTCTC | 2 cut(s) | 12, 297 |
| BstMBI | GATC | 1 cut(s) | 372 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 109, 479 |
| BstNI | CCWGG | 1 cut(s) | 114 |
| BstNSI | RCATGY | 1 cut(s) | 37 |
| BstSCI | CCNGG | 1 cut(s) | 112 |
| Bsu36I | CCTNAGG | 3 cut(s) | 183, 243, 300 |
| BsuI | GTATCC | 1 cut(s) | 391 |
| BsuRI | GGCC | 3 cut(s) | 112, 131, 312 |
| BtsCI | GGATG | 2 cut(s) | 132, 238 |
| Cac8I | GCNNGC | 2 cut(s) | 105, 168 |
| Cfr13I | GGNCC | 2 cut(s) | 110, 310 |
| CseI | GACGC | 1 cut(s) | 428 |
| CviAII | CATG | 1 cut(s) | 34 |
| DdeI | CTNAG | 4 cut(s) | 50, 183, 243, 300 |
| DpnI | GATC | 1 cut(s) | 374 |
| DpnII | GATC | 1 cut(s) | 372 |
| DraIII | CACNNNGTG | 1 cut(s) | 452 |
| EaeI | YGGCCR | 1 cut(s) | 129 |
| Eam1104I | CTCTTC | 1 cut(s) | 311 |
| EarI | CTCTTC | 1 cut(s) | 311 |
| EciI | GGCGGA | 1 cut(s) | 284 |
| Eco32I | GATATC | 1 cut(s) | 357 |
| Eco81I | CCTNAGG | 3 cut(s) | 183, 243, 300 |
| EcoRII | CCWGG | 1 cut(s) | 112 |
| EcoRV | GATATC | 1 cut(s) | 357 |
| Esp3I | CGTCTC | 1 cut(s) | 12 |
| FaeI | CATG | 1 cut(s) | 37 |
| FaiI | YATR | 5 cut(s) | 35, 206, 431, 433, 435 |
| FatI | CATG | 1 cut(s) | 33 |
| FblI | GTMKAC | 1 cut(s) | 417 |
| Fnu4HI | GCNGC | 1 cut(s) | 90 |
| FokI | GGATG | 2 cut(s) | 119, 225 |
| Fsp4HI | GCNGC | 1 cut(s) | 90 |
| FspBI | CTAG | 2 cut(s) | 104, 351 |
| GluI | GCNGC | 1 cut(s) | 90 |
| GsaI | CCCAGC | 1 cut(s) | 111 |
| HaeIII | GGCC | 3 cut(s) | 112, 131, 312 |
| HgaI | GACGC | 1 cut(s) | 428 |
| Hin1I | GRCGYC | 1 cut(s) | 5 |
| Hin1II | CATG | 1 cut(s) | 37 |
| HphI | GGTGA | 3 cut(s) | 316, 464, 473 |
| Hpy166II | GTNNAC | 1 cut(s) | 418 |
| Hpy188I | TCNGA | 1 cut(s) | 488 |
| Hpy188III | TCNNGA | 2 cut(s) | 185, 253 |
| Hpy8I | GTNNAC | 1 cut(s) | 418 |
| HpyAV | CCTTC | 2 cut(s) | 223, 301 |
| HpyCH4III | ACNGT | 2 cut(s) | 403, 411 |
| HpyCH4IV | ACGT | 1 cut(s) | 5 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 109, 479 |
| HpyF3I | CTNAG | 4 cut(s) | 50, 183, 243, 300 |
| HpySE526I | ACGT | 1 cut(s) | 5 |
| Hsp92I | GRCGYC | 1 cut(s) | 5 |
| Hsp92II | CATG | 1 cut(s) | 37 |
| Kzo9I | GATC | 1 cut(s) | 372 |
| LmnI | GCTCC | 1 cut(s) | 175 |
| LpnPI | CCDG | 8 cut(s) | 93, 99, 113, 126, 170, 180, 326, 459 |
| MaeI | CTAG | 2 cut(s) | 104, 351 |
| MaeII | ACGT | 1 cut(s) | 5 |
| MaeIII | GTNAC | 3 cut(s) | 29, 77, 123 |
| MalI | GATC | 1 cut(s) | 374 |
| MboI | GATC | 1 cut(s) | 372 |
| MboII | GAAGA | 4 cut(s) | 130, 238, 328, 476 |
| MfeI | CAATTG | 1 cut(s) | 153 |
| MlsI | TGGCCA | 1 cut(s) | 131 |
| MluCI | AATT | 1 cut(s) | 153 |
| MluNI | TGGCCA | 1 cut(s) | 131 |
| MmeI | TCCRAC | 1 cut(s) | 214 |
| Mox20I | TGGCCA | 1 cut(s) | 131 |
| MscI | TGGCCA | 1 cut(s) | 131 |
| MseI | TTAA | 2 cut(s) | 17, 264 |
| Msp20I | TGGCCA | 1 cut(s) | 131 |
| MspR9I | CCNGG | 1 cut(s) | 114 |
| MunI | CAATTG | 1 cut(s) | 153 |
| MvaI | CCWGG | 1 cut(s) | 114 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 109, 479 |
| NdeII | GATC | 1 cut(s) | 372 |
| NheI | GCTAGC | 1 cut(s) | 103 |
| NlaIII | CATG | 1 cut(s) | 37 |
| NlaIV | GGNNCC | 1 cut(s) | 369 |
| NmuCI | GTSAC | 1 cut(s) | 77 |
| NspI | RCATGY | 1 cut(s) | 37 |
| PciI | ACATGT | 1 cut(s) | 33 |
| PkrI | GCNGC | 1 cut(s) | 91 |
| PscI | ACATGT | 1 cut(s) | 33 |
| Psp6I | CCWGG | 1 cut(s) | 112 |
| PspFI | CCCAGC | 1 cut(s) | 107 |
| PspGI | CCWGG | 1 cut(s) | 112 |
| PspN4I | GGNNCC | 1 cut(s) | 369 |
| PspPI | GGNCC | 2 cut(s) | 110, 310 |
| SaqAI | TTAA | 2 cut(s) | 17, 264 |
| SatI | GCNGC | 1 cut(s) | 90 |
| Sau3AI | GATC | 1 cut(s) | 372 |
| Sau96I | GGNCC | 2 cut(s) | 110, 310 |
| ScrFI | CCNGG | 1 cut(s) | 114 |
| Sse9I | AATT | 1 cut(s) | 153 |
| SsiI | CCGC | 2 cut(s) | 89, 269 |
| SspMI | CTAG | 2 cut(s) | 104, 351 |
| StyD4I | CCNGG | 1 cut(s) | 112 |
| TaaI | ACNGT | 2 cut(s) | 403, 411 |
| TaiI | ACGT | 1 cut(s) | 8 |
| TasI | AATT | 1 cut(s) | 153 |
| TauI | GCSGC | 1 cut(s) | 92 |
| Tru1I | TTAA | 2 cut(s) | 17, 264 |
| Tru9I | TTAA | 2 cut(s) | 17, 264 |
| TseFI | GTSAC | 1 cut(s) | 77 |
| Tsp45I | GTSAC | 1 cut(s) | 77 |
| TspDTI | ATGAA | 1 cut(s) | 431 |
| XceI | RCATGY | 1 cut(s) | 37 |
| XmiI | GTMKAC | 1 cut(s) | 417 |
| XspI | CTAG | 2 cut(s) | 104, 351 |
| ZraI | GACGTC | 1 cut(s) | 6 |