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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 220 |
| AciI | CCGC | 3 cut(s) | 491, 624, 657 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 26 |
| AgeI | ACCGGT | 1 cut(s) | 586 |
| AgsI | TTSAA | 3 cut(s) | 201, 257, 310 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 106 |
| AjnI | CCWGG | 2 cut(s) | 127, 237 |
| AluBI | AGCT | 4 cut(s) | 83, 323, 454, 569 |
| AluI | AGCT | 4 cut(s) | 83, 323, 454, 569 |
| Alw26I | GTCTC | 2 cut(s) | 44, 645 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 110 |
| AoxI | GGCC | 2 cut(s) | 527, 558 |
| ApeKI | GCWGC | 3 cut(s) | 64, 320, 566 |
| AsiGI | ACCGGT | 1 cut(s) | 586 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 182 |
| AspA2I | CCTAGG | 1 cut(s) | 530 |
| AspS9I | GGNCC | 1 cut(s) | 584 |
| AvaII | GGWCC | 1 cut(s) | 584 |
| AvrII | CCTAGG | 1 cut(s) | 530 |
| BanI | GGYRCC | 1 cut(s) | 220 |
| BbvI | GCAGC | 3 cut(s) | 51, 332, 578 |
| BccI | CCATC | 2 cut(s) | 53, 413 |
| BciT130I | CCWGG | 2 cut(s) | 129, 239 |
| BciVI | GTATCC | 1 cut(s) | 235 |
| BcoDI | GTCTC | 2 cut(s) | 44, 645 |
| BfaI | CTAG | 2 cut(s) | 162, 531 |
| BfuI | GTATCC | 1 cut(s) | 235 |
| BisI | GCNGC | 5 cut(s) | 65, 321, 492, 567, 625 |
| BlnI | CCTAGG | 1 cut(s) | 530 |
| BlsI | GCNGC | 5 cut(s) | 66, 322, 493, 568, 626 |
| Bme1390I | CCNGG | 2 cut(s) | 129, 239 |
| Bme18I | GGWCC | 1 cut(s) | 584 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 106 |
| BmgT120I | GGNCC | 1 cut(s) | 584 |
| BmiI | GGNNCC | 2 cut(s) | 18, 222 |
| BmrFI | CCNGG | 2 cut(s) | 129, 239 |
| BmsI | GCATC | 1 cut(s) | 553 |
| BpuEI | CTTGAG | 2 cut(s) | 450, 524 |
| BsaJI | CCNNGG | 2 cut(s) | 486, 530 |
| BsaWI | WCCGGW | 1 cut(s) | 586 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 26 |
| Bse118I | RCCGGY | 1 cut(s) | 586 |
| BseBI | CCWGG | 2 cut(s) | 129, 239 |
| BseDI | CCNNGG | 2 cut(s) | 486, 530 |
| BseGI | GGATG | 2 cut(s) | 64, 424 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 26 |
| BseMII | CTCAG | 3 cut(s) | 39, 363, 588 |
| BseXI | GCAGC | 3 cut(s) | 51, 332, 578 |
| BseYI | CCCAGC | 1 cut(s) | 569 |
| BsgI | GTGCAG | 1 cut(s) | 227 |
| BshFI | GGCC | 2 cut(s) | 529, 560 |
| BshNI | GGYRCC | 1 cut(s) | 220 |
| BshTI | ACCGGT | 1 cut(s) | 586 |
| BsiSI | CCGG | 1 cut(s) | 587 |
| BslFI | GGGAC | 1 cut(s) | 43 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 26 |
| BsmAI | GTCTC | 2 cut(s) | 44, 645 |
| BsmBI | CGTCTC | 1 cut(s) | 44 |
| BsmFI | GGGAC | 1 cut(s) | 43 |
| BsnI | GGCC | 2 cut(s) | 529, 560 |
| Bsp143I | GATC | 1 cut(s) | 148 |
| BspACI | CCGC | 3 cut(s) | 491, 624, 657 |
| BspANI | GGCC | 2 cut(s) | 529, 560 |
| BspCNI | CTCAG | 3 cut(s) | 40, 364, 589 |
| BspHI | TCATGA | 1 cut(s) | 97 |
| BspLI | GGNNCC | 2 cut(s) | 18, 222 |
| BspT107I | GGYRCC | 1 cut(s) | 220 |
| BsrFI | RCCGGY | 1 cut(s) | 586 |
| BssAI | RCCGGY | 1 cut(s) | 586 |
| BssECI | CCNNGG | 2 cut(s) | 486, 530 |
| BssMI | GATC | 1 cut(s) | 148 |
| BssT1I | CCWWGG | 2 cut(s) | 486, 530 |
| Bst2UI | CCWGG | 2 cut(s) | 129, 239 |
| Bst4CI | ACNGT | 3 cut(s) | 295, 351, 654 |
| Bst6I | CTCTTC | 2 cut(s) | 381, 536 |
| BstDEI | CTNAG | 6 cut(s) | 48, 246, 336, 372, 441, 597 |
| BstF5I | GGATG | 2 cut(s) | 64, 424 |
| BstKTI | GATC | 1 cut(s) | 151 |
| BstMAI | GTCTC | 2 cut(s) | 44, 645 |
| BstMBI | GATC | 1 cut(s) | 148 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 61, 566 |
| BstNI | CCWGG | 2 cut(s) | 129, 239 |
| BstSCI | CCNGG | 2 cut(s) | 127, 237 |
| BstV1I | GCAGC | 3 cut(s) | 51, 332, 578 |
| BsuI | GTATCC | 1 cut(s) | 235 |
| BsuRI | GGCC | 2 cut(s) | 529, 560 |
| BtsCI | GGATG | 2 cut(s) | 64, 424 |
| BtsIMutI | CAGTG | 1 cut(s) | 650 |
| CaiI | CAGNNNCTG | 1 cut(s) | 110 |
| CciI | TCATGA | 1 cut(s) | 97 |
| Cfr10I | RCCGGY | 1 cut(s) | 586 |
| Cfr13I | GGNCC | 1 cut(s) | 584 |
| CseI | GACGC | 2 cut(s) | 61, 78 |
| CspAI | ACCGGT | 1 cut(s) | 586 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 21, 56 |
| CviAII | CATG | 4 cut(s) | 68, 86, 98, 628 |
| DdeI | CTNAG | 6 cut(s) | 48, 246, 336, 372, 441, 597 |
| DpnI | GATC | 1 cut(s) | 150 |
| DpnII | GATC | 1 cut(s) | 148 |
| DriI | GACNNNNNGTC | 1 cut(s) | 106 |
| Eam1104I | CTCTTC | 2 cut(s) | 381, 536 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 106 |
| EarI | CTCTTC | 2 cut(s) | 381, 536 |
| Eco130I | CCWWGG | 2 cut(s) | 486, 530 |
| Eco147I | AGGCCT | 1 cut(s) | 529 |
| Eco47I | GGWCC | 1 cut(s) | 584 |
| EcoRII | CCWGG | 2 cut(s) | 127, 237 |
| EcoT14I | CCWWGG | 2 cut(s) | 486, 530 |
| ErhI | CCWWGG | 2 cut(s) | 486, 530 |
| Esp3I | CGTCTC | 1 cut(s) | 44 |
| FaeI | CATG | 4 cut(s) | 71, 89, 101, 631 |
| FaiI | YATR | 6 cut(s) | 69, 87, 99, 158, 548, 629 |
| FaqI | GGGAC | 1 cut(s) | 43 |
| FatI | CATG | 4 cut(s) | 67, 85, 97, 627 |
| Fnu4HI | GCNGC | 5 cut(s) | 65, 321, 492, 567, 625 |
| FokI | GGATG | 2 cut(s) | 71, 431 |
| Fsp4HI | GCNGC | 5 cut(s) | 65, 321, 492, 567, 625 |
| FspBI | CTAG | 2 cut(s) | 162, 531 |
| GluI | GCNGC | 5 cut(s) | 65, 321, 492, 567, 625 |
| GsaI | CCCAGC | 1 cut(s) | 573 |
| HaeIII | GGCC | 2 cut(s) | 529, 560 |
| HapII | CCGG | 1 cut(s) | 587 |
| HgaI | GACGC | 2 cut(s) | 61, 78 |
| Hin1II | CATG | 4 cut(s) | 71, 89, 101, 631 |
| HindIII | AAGCTT | 1 cut(s) | 452 |
| HinfI | GANTC | 3 cut(s) | 268, 436, 638 |
| HpaII | CCGG | 1 cut(s) | 587 |
| Hpy188I | TCNGA | 3 cut(s) | 273, 402, 598 |
| Hpy188III | TCNNGA | 4 cut(s) | 20, 98, 371, 503 |
| HpyAV | CCTTC | 6 cut(s) | 125, 274, 506, 550, 626, 654 |
| HpyCH4III | ACNGT | 3 cut(s) | 295, 351, 654 |
| HpyCH4IV | ACGT | 1 cut(s) | 313 |
| HpyCH4V | TGCA | 6 cut(s) | 37, 67, 144, 169, 208, 566 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 61, 566 |
| HpyF3I | CTNAG | 6 cut(s) | 48, 246, 336, 372, 441, 597 |
| HpySE526I | ACGT | 1 cut(s) | 313 |
| Hsp92II | CATG | 4 cut(s) | 71, 89, 101, 631 |
| Kzo9I | GATC | 1 cut(s) | 148 |
| Lsp1109I | GCAGC | 3 cut(s) | 51, 332, 578 |
| LweI | GCATC | 1 cut(s) | 553 |
| MaeI | CTAG | 2 cut(s) | 162, 531 |
| MaeII | ACGT | 1 cut(s) | 313 |
| MaeIII | GTNAC | 5 cut(s) | 90, 331, 375, 405, 433 |
| MalI | GATC | 1 cut(s) | 150 |
| MboI | GATC | 1 cut(s) | 148 |
| MboII | GAAGA | 4 cut(s) | 262, 398, 553, 628 |
| MluCI | AATT | 4 cut(s) | 123, 153, 178, 190 |
| MlyI | GAGTC | 2 cut(s) | 430, 647 |
| MmeI | TCCRAC | 1 cut(s) | 489 |
| MnlI | CCTC | 5 cut(s) | 234, 322, 371, 474, 592 |
| MroXI | GAANNNNTTC | 1 cut(s) | 182 |
| MseI | TTAA | 2 cut(s) | 122, 667 |
| MslI | CAYNNNNRTG | 2 cut(s) | 401, 417 |
| MspA1I | CMGCKG | 1 cut(s) | 569 |
| MspI | CCGG | 1 cut(s) | 587 |
| MspR9I | CCNGG | 2 cut(s) | 129, 239 |
| MvaI | CCWGG | 2 cut(s) | 129, 239 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 61, 566 |
| NdeII | GATC | 1 cut(s) | 148 |
| NlaIII | CATG | 4 cut(s) | 71, 89, 101, 631 |
| NlaIV | GGNNCC | 2 cut(s) | 18, 222 |
| NmuCI | GTSAC | 2 cut(s) | 90, 433 |
| PagI | TCATGA | 1 cut(s) | 97 |
| PceI | AGGCCT | 1 cut(s) | 529 |
| PdmI | GAANNNNTTC | 1 cut(s) | 182 |
| PfeI | GAWTC | 1 cut(s) | 268 |
| PinAI | ACCGGT | 1 cut(s) | 586 |
| PkrI | GCNGC | 5 cut(s) | 66, 322, 493, 568, 626 |
| PleI | GAGTC | 2 cut(s) | 430, 646 |
| PpsI | GAGTC | 2 cut(s) | 430, 646 |
| Psp6I | CCWGG | 2 cut(s) | 127, 237 |
| PspFI | CCCAGC | 1 cut(s) | 569 |
| PspGI | CCWGG | 2 cut(s) | 127, 237 |
| PspN4I | GGNNCC | 2 cut(s) | 18, 222 |
| PspPI | GGNCC | 1 cut(s) | 584 |
| PstNI | CAGNNNCTG | 1 cut(s) | 110 |
| PvuII | CAGCTG | 1 cut(s) | 569 |
| RseI | CAYNNNNRTG | 2 cut(s) | 401, 417 |
| SaqAI | TTAA | 2 cut(s) | 122, 667 |
| SatI | GCNGC | 5 cut(s) | 65, 321, 492, 567, 625 |
| Sau3AI | GATC | 1 cut(s) | 148 |
| Sau96I | GGNCC | 1 cut(s) | 584 |
| SchI | GAGTC | 2 cut(s) | 430, 647 |
| ScrFI | CCNGG | 2 cut(s) | 129, 239 |
| SfaNI | GCATC | 1 cut(s) | 553 |
| SinI | GGWCC | 1 cut(s) | 584 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 401, 417 |
| SmlI | CTYRAG | 2 cut(s) | 465, 503 |
| SmoI | CTYRAG | 2 cut(s) | 465, 503 |
| Sse9I | AATT | 4 cut(s) | 123, 153, 178, 190 |
| SseBI | AGGCCT | 1 cut(s) | 529 |
| SsiI | CCGC | 3 cut(s) | 491, 624, 657 |
| SspMI | CTAG | 2 cut(s) | 162, 531 |
| StuI | AGGCCT | 1 cut(s) | 529 |
| StyD4I | CCNGG | 2 cut(s) | 127, 237 |
| StyI | CCWWGG | 2 cut(s) | 486, 530 |
| TaaI | ACNGT | 3 cut(s) | 295, 351, 654 |
| TaiI | ACGT | 1 cut(s) | 316 |
| TaqI | TCGA | 1 cut(s) | 226 |
| TasI | AATT | 4 cut(s) | 123, 153, 178, 190 |
| TauI | GCSGC | 2 cut(s) | 494, 627 |
| TfiI | GAWTC | 1 cut(s) | 268 |
| Tru1I | TTAA | 2 cut(s) | 122, 667 |
| Tru9I | TTAA | 2 cut(s) | 122, 667 |
| TscAI | CASTG | 1 cut(s) | 657 |
| TseFI | GTSAC | 2 cut(s) | 90, 433 |
| TseI | GCWGC | 3 cut(s) | 64, 320, 566 |
| Tsp45I | GTSAC | 2 cut(s) | 90, 433 |
| TspDTI | ATGAA | 1 cut(s) | 629 |
| TspRI | CASTG | 1 cut(s) | 657 |
| VpaK11BI | GGWCC | 1 cut(s) | 584 |
| XmaJI | CCTAGG | 1 cut(s) | 530 |
| XmnI | GAANNNNTTC | 1 cut(s) | 182 |
| XspI | CTAG | 2 cut(s) | 162, 531 |