Rorug02G0222000

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000049
Physical Location & Seq
Reverse (-)
21475429 .. 21492398
16970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0222000.1

Sequence Viewer

Length: 2220 bp
ATGGTTTCCTCCGCCGTTCCTATTTCTGACACCTTGAAGAAAATATGGGATAAGTGGAACCTCCGGGGCATCATCATATTAAGCCTCTTCTTGCAGGTCTTTTTAATTTTTTGTGCGCCCCTGAGAAAGAGAACATCAAGCAAATGGGTGATCGTGCCCATTTGGTTATCTTACTTGCTCGCAGACTGGGCTGCTAACTTTGCCATTGGATTAATTGCAAGCAGCCAAACTGATACCAACGGTCGCCGAGACAATGGCGGCCTTTTGGCATTTTGGGCTCCATTTCTTTTGATACACTTGGGTGGTCCGGACACCATAATTGCGATTTCTATGGAAGATAACGCCTTGTGGCCAAGACACTTTCTTGGTCTCATATTCCAGGTTTTGGCTGTGGCGTACGTCTTCACCATGCAGTCAACTCGAGAAAACAAGCTATGGTGGCCAACACTGTTGCTAATTTTCCCAGGACTAGTCAAGTATGGCGAGCGAACACGTGCTTTATATCTTGCCAGCTTAAGCAGTCTCAAGGACTCCATACTTGCAGTACCGGACACTGGACCGGATTATGCAAAATACATGGATGAGTACTCCTCAAGAAAAGATGCCAATCTTCCAATTCATATACACACCCACGAAGGCCGTGGGTCAGCTGAATCCAAGATTGAGACTCACCCTGTGGAAGAAGGCCCCGACAACATTAAAAATATTGCTGTGGTGCAGAAGGCTTATCAATTATTTGAAATCTCCATGGGTCTTATTATAGATCTTCTTTTTGACTTTCATCAGCGGAACGAGAGCCGTGCCTATTTCCTTAGGACAACCGCAGAAGAGGCTTATACATTAATCGCAACTGAGCTCAACTTTATGTATGAAGCTCTTTACACTAAAGCTCATGTGGTGCATTCTGTGTGGGGATATGTTTTAAGGGCTACATCATTTGGAACGGTCTGGATAGCCCTTGGTTTCTTCTATAAGTTGGACAAACATGGCTTCCACAGGTTTGATGTTAGAGTAACCTATTCCTTGCTCTTGGGTGCCATAGCCTTGGATTCAATAGCTATTTCGATGCTTATCCTATCTGATTGGACTGTCGTAAAGTGTGGCCAGAAGTGTTGTTCTTTAGCCACTATTCTCATGGACAAACACCTCAACTTGAAGAGAGCTTGGTGGCGTCCTACTGAAAAGTGGAAGATGTTATTTGGAAGGTGGTCTGAATCTGTCTCTACGTTCAGCTTCATAAATTATTCTCTCAAAGAATGCAGAAGAGTGCCACCTACAGTTATGGACTATGTAGGCCTTTTGGGCACTAAAATAATCAGAATCTTGTGGCTCAACGTTATTTGGGTTGAGATGAAATATATAATTATATATAGACTCCGCGACTCTGGGGAACTGTGGAAGTTCATTTTTGAAGAGTTGAAAAGTAAATCGGAGGTTGCAGTTGATCCTCAAAATGGCAGCAAAATATGTTCAGCAAGAGGGGAGTGGATTCTGAAGAAGTATAAGGAGAACTACTATGGGGATAAGCCACAGGAGTATGATAAATTAAGATCGCAAGTTTATCTTGGTGATGATGTTACTTATGACCGACGAATTCTGTTGTGGCACATTGCAACCGAGTTGCGCTATTATGATGAGAAGAAGGAGAGCAATGCTGGTGATGAGAAGAAGAGCAATGCTCATCGAGAGTATAGTAAGACATTGTCTGATTATATGTTATATCTCTTAGTAATGCAGCCTGATTTGATGTCTTCTGTAGCGGGAATTGGTCAGAAAAGATTTTCTGATACTTTTGCAGAAGCCAAACAATTCTTTGAAAAAAGGGCACTGAGATCGGATGCAACACCAGAAGATGCTTGTGGAAAACTCCTCAAAGTAAATACAGAAATTAAACCCGTTAATGTCAAAGGGAATAGAAGCAAGTCTGTATTGTTTGATGCATGTATTCTGGCAAAGGAGCTGAAGAATTTGGAATGGAAATTGATAAGTGAAGTGTGGGTGGAATTACTATCATATGCTGCAAGTCATTGCAGACCAAGTAGCCATGCCCAATTGCTGAGTAAAGGTGGAGAGCTTGTCACTTTTGTTTGGTTATTAATGGCTCATTTTGGCATAGCAGAGCAGTTCCAAGTGCTAGAGGTCCAAGCAAGAGTAAAACTGATAGTTGAAAAGCCACCAGGTGAATCCTTTTCTTGGTCGGGTGAAAAGCCACCAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

739

Amino Acids

84.8

Weight (kDa)

8.83

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4220 PF13968 55 - 415 1.2e-88 Domain of unknown function (DUF4220)
DUF594 PF04578 656 - 705 7e-23 Protein of unknown function, DUF594
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000049)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09850 FvH4_2g12512 FvH4_2g19830 FvH4_2g21281 FvH4_2g30300 FvH4_3g04012 FvH4_3g12601 FvH4_3g25620 FvH4_3g28881 FvH4_3g29932 FvH4_4g12651 FvH4_5g27100 FvH4_6g03011 FvH4_6g10710 FvH4_6g10711 FvH4_6g19991 FvH4_6g25461 FvH4_6g32661 FvH4_7g16371
rosa_chinensis RchiOBHm_Chr1g0347111 RchiOBHm_Chr1g0350781 RchiOBHm_Chr1g0350971 RchiOBHm_Chr2g0123951 RchiOBHm_Chr4g0397591 RchiOBHm_Chr5g0032121 RchiOBHm_Chr6g0265951
rosa_laevigata RLG00000000709 RLG00000001093 RLG00000001244 RLG00000002381 RLG00000003581 RLG00000003584 RLG00000004011 RLG00000004587 RLG00000005734 RLG00000007804 RLG00000008145 RLG00000008422 RLG00000008949 RLG00000009240 RLG00000009334 RLG00000009773 RLG00000010531 RLG00000012000 RLG00000012913 RLG00000013188 RLG00000013289 RLG00000013302 RLG00000013943 RLG00000016032 RLG00000016277 RLG00000016479 RLG00000017591 RLG00000017899 RLG00000018452 RLG00000018455 RLG00000019343 RLG00000021649 RLG00000023222 RLG00000023677 RLG00000024058 RLG00000024433 RLG00000026719 RLG00000027778 RLG00000028367 RLG00000028761 RLG00000029079 RLG00000030238 RLG00000030452 RLG00000030719 RLG00000030834 RLG00000031739 RLG00000032099 RLG00000033644 RLG00000033713 RLG00000034081 RLG00000035108 RLG00000035250 RLG00000035711 RLG00000036556 RLG00000036747
rosa_multiflora Rmu_co8042282.1_g000001 Rmu_co8251111.1_g000001 Rmu_co8268445.1_g000001 Rmu_co8273215.1_g000001 Rmu_co8469153.1_g000002 Rmu_sc0000035.1_g000023 Rmu_sc0000092.1_g000002 Rmu_sc0000166.1_g000008 Rmu_sc0000260.1_g000017 Rmu_sc0000317.1_g000014 Rmu_sc0000470.1_g000023 Rmu_sc0000533.1_g000060 Rmu_sc0000539.1_g000011 Rmu_sc0000546.1_g000022 Rmu_sc0000571.1_g000022 Rmu_sc0000610.1_g000019 Rmu_sc0000706.1_g000038 Rmu_sc0000843.1_g000005 Rmu_sc0000882.1_g000008 Rmu_sc0000928.1_g000014 Rmu_sc0000928.1_g000015 Rmu_sc0001140.1_g000009 Rmu_sc0001161.1_g000015 Rmu_sc0001179.1_g000041 Rmu_sc0001296.1_g000014 Rmu_sc0001373.1_g000023 Rmu_sc0001433.1_g000015 Rmu_sc0001519.1_g000009 Rmu_sc0001878.1_g000011 Rmu_sc0002112.1_g000014 Rmu_sc0002141.1_g000030 Rmu_sc0002148.1_g000029 Rmu_sc0002156.1_g000014 Rmu_sc0002199.1_g000007 Rmu_sc0002586.1_g000033 Rmu_sc0002620.1_g000008 Rmu_sc0002646.1_g000010 Rmu_sc0002800.1_g000026 Rmu_sc0002921.1_g000001 Rmu_sc0002964.1_g000009 Rmu_sc0002989.1_g000009 Rmu_sc0003133.1_g000002 Rmu_sc0003238.1_g000030 Rmu_sc0003314.1_g000011 Rmu_sc0003314.1_g000012 Rmu_sc0003314.1_g000028 Rmu_sc0003458.1_g000003 Rmu_sc0003499.1_g000034 Rmu_sc0003610.1_g000040 Rmu_sc0003859.1_g000006 Rmu_sc0003971.1_g000002 Rmu_sc0004161.1_g000006 Rmu_sc0004250.1_g000023 Rmu_sc0004467.1_g000071 Rmu_sc0004498.1_g000004 Rmu_sc0004521.1_g000024 Rmu_sc0004529.1_g000030 Rmu_sc0004547.1_g000009 Rmu_sc0004835.1_g000034 Rmu_sc0004944.1_g000012 Rmu_sc0005030.1_g000005 Rmu_sc0005030.1_g000006 Rmu_sc0005137.1_g000033 Rmu_sc0005294.1_g000013 Rmu_sc0005416.1_g000020 Rmu_sc0005426.1_g000010 Rmu_sc0005432.1_g000001 Rmu_sc0005450.1_g000020 Rmu_sc0005566.1_g000008 Rmu_sc0005693.1_g000020 Rmu_sc0005992.1_g000007 Rmu_sc0006007.1_g000001 Rmu_sc0006016.1_g000017 Rmu_sc0006016.1_g000018 Rmu_sc0006147.1_g000009 Rmu_sc0006536.1_g000032 Rmu_sc0006536.1_g000033 Rmu_sc0006572.1_g000006 Rmu_sc0007474.1_g000013 Rmu_sc0007490.1_g000008 Rmu_sc0007490.1_g000009 Rmu_sc0007618.1_g000003 Rmu_sc0007753.1_g000021 Rmu_sc0008039.1_g000001 Rmu_sc0008044.1_g000008 Rmu_sc0008155.1_g000003 Rmu_sc0008308.1_g000006 Rmu_sc0008308.1_g000017 Rmu_sc0008360.1_g000006 Rmu_sc0008380.1_g000015 Rmu_sc0008596.1_g000002 Rmu_sc0008596.1_g000003 Rmu_sc0008629.1_g000013 Rmu_sc0008991.1_g000011 Rmu_sc0009015.1_g000004 Rmu_sc0009015.1_g000005 Rmu_sc0009360.1_g000050 Rmu_sc0009386.1_g000009 Rmu_sc0009410.1_g000003 Rmu_sc0009410.1_g000006 Rmu_sc0009609.1_g000001 Rmu_sc0010317.1_g000003 Rmu_sc0010871.1_g000016 Rmu_sc0010999.1_g000018 Rmu_sc0011019.1_g000009 Rmu_sc0011343.1_g000014 Rmu_sc0011578.1_g000019 Rmu_sc0011781.1_g000012 Rmu_sc0011921.1_g000008 Rmu_sc0012005.1_g000001 Rmu_sc0012179.1_g000006 Rmu_sc0012211.1_g000007 Rmu_sc0012220.1_g000002 Rmu_sc0012742.1_g000001 Rmu_sc0013620.1_g000004 Rmu_sc0014003.1_g000007 Rmu_sc0014203.1_g000024 Rmu_sc0014612.1_g000004 Rmu_sc0014667.1_g000006 Rmu_sc0015359.1_g000002 Rmu_sc0016526.1_g000003 Rmu_sc0017253.1_g000008 Rmu_sc0017394.1_g000005 Rmu_sc0017394.1_g000006 Rmu_sc0018840.1_g000001 Rmu_sc0019315.1_g000001 Rmu_sc0022276.1_g000002 Rmu_sc0024289.1_g000001 Rmu_sc0024918.1_g000004 Rmu_sc0026424.1_g000001 Rmu_sc0026425.1_g000001 Rmu_sc0030616.1_g000001 Rmu_sc0030617.1_g000001 Rmu_sc0031931.1_g000003 Rmu_sc0034665.1_g000001 Rmu_sc0035393.1_g000001 Rmu_ssc0000162.1_g000015 Rmu_ssc0000255.1_g000060 Rmu_ssc0000409.1_g000012
rosa_roxburghii Rroxscaffold_1G00006160 Rroxscaffold_2G00084140 Rroxscaffold_2G00109070 Rroxscaffold_2G00136190 Rroxscaffold_2G00136200 Rroxscaffold_3G00237880 Rroxscaffold_3G00250500 Rroxscaffold_3G00272860 Rroxscaffold_4G00283710 Rroxscaffold_4G00308190 Rroxscaffold_5G00334250 Rroxscaffold_5G00339840 Rroxscaffold_5G00380790 Rroxscaffold_7G00177140 Rroxscaffold_7G00186880 Rroxscaffold_7G00201080
rosa_rugosa Rorug01G0002600 Rorug01G0159800.1 Rorug01G0164900 Rorug01G0176500 Rorug01G0185400 Rorug01G0223500 Rorug01G0231900 Rorug01G0251700 Rorug01G0283600 Rorug01G0309600 Rorug01G0333600 Rorug01G0478500 Rorug01G0478700 Rorug01G0483600 Rorug02G0000400 Rorug02G0025400 Rorug02G0025500 Rorug02G0160600 Rorug02G0179800 Rorug02G0221000 Rorug02G0222000 Rorug02G0239900 Rorug02G0291000 Rorug02G0292800 Rorug02G0299400 Rorug02G0361300 Rorug02G0382000 Rorug02G0438400 Rorug02G0438400 Rorug02G0463000 Rorug02G0599300 Rorug03G0085500 Rorug03G0143300 Rorug03G0150200 Rorug03G0214500 Rorug03G0301800 Rorug03G0304100 Rorug03G0304200 Rorug03G0318900 Rorug03G0342400 Rorug03G0363300.1 Rorug04G0006400 Rorug04G0026200 Rorug04G0185500 Rorug04G0185600 Rorug04G0242600 Rorug04G0294300 Rorug04G0322000 Rorug04G0380900 Rorug05G0050300 Rorug05G0050300 Rorug05G0086800 Rorug05G0106900 Rorug05G0166700 Rorug05G0220300 Rorug05G0237400 Rorug05G0296300 Rorug05G0313400 Rorug05G0323000 Rorug05G0347200 Rorug05G0363800 Rorug05G0413500 Rorug05G0440900 Rorug05G0498500 Rorug05G0576300 Rorug06G0025600 Rorug06G0029800 Rorug06G0045700 Rorug06G0087400 Rorug06G0087600 Rorug06G0108200 Rorug06G0110200 Rorug06G0129900 Rorug06G0129900 Rorug06G0133800 Rorug06G0141200 Rorug06G0219200 Rorug06G0268900 Rorug06G0269000 Rorug06G0291100 Rorug06G0479100 Rorug06G0479200 Rorug06G0511800 Rorug07G0028800.1 Rorug07G0052500 Rorug07G0071900 Rorug07G0076600 Rorug07G0079900 Rorug07G0085200 Rorug07G0302400 Rorug07G0303700 Rorug07G0318000
rosa_samantha Rh1AG194500 Rh1AG194600 Rh1AG354700 Rh1BG147200 Rh1BG169000 Rh1BG212500 Rh1BG241800 Rh1CG179300 Rh2AG232000 Rh2BG307200 Rh2BG352400 Rh2BG563400 Rh2CG200100 Rh2CG285300 Rh2CG331100 Rh2CG533700 Rh2CG574800 Rh2CG650600 Rh2CG650700 Rh2DG212200 Rh3BG142200 Rh3BG294100 Rh3BG300500 Rh3CG143500 Rh3DG143400 Rh4AG079100 Rh4AG125800 Rh4BG119600 Rh4BG187000 Rh4BG248400 Rh4CG036500 Rh4CG084700 Rh4CG134000 Rh4CG198900 Rh5AG384400 Rh5BG212700 Rh5CG235900 Rh5CG292600 Rh5CG401000 Rh5CG502100 Rh5CG506700 Rh5DG020900 Rh5DG269500 Rh5DG330300 Rh5DG388800 Rh6AG031600 Rh6AG257800 Rh6AG375600 Rh6BG338400 Rh6BG418300 Rh6CG280900 Rh6DG411200 Rh7AG075900 Rh7BG073500 Rh7BG088700 Rh7BG210200 Rh7BG260400 Rh7BG269600 Rh7BG270600 Rh7BG459100 Rh7BG459200 Rh7CG258100
rosa_wichuraiana Rw0G014160 Rw0G019160 Rw1G007800 Rw1G013530 Rw1G036200 Rw2G016380 Rw2G022170 Rw2G036380 Rw3G001880 Rw4G026250 Rw5G017280 Rw5G045990 Rw6G019190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 85
AccB1I GGYRCC 1 cut(s) 1034
AccB7I CCANNNNNTGG 1 cut(s) 385
AccII CGCG 1 cut(s) 1380
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 6 cut(s) 12, 258, 787, 822, 1378, 1760
AclI AACGTT 1 cut(s) 1335
AclWI GGATC 1 cut(s) 1439
AcoI YGGCCR 3 cut(s) 350, 440, 1102
AcsI RAATTY 2 cut(s) 1593, 1966
AcuI CTGAAG 2 cut(s) 1514, 1982
AcvI CACGTG 1 cut(s) 494
AcyI GRCGYC 1 cut(s) 1171
AdeI CACNNNGTG 2 cut(s) 676, 2180
AfaI GTAC 3 cut(s) 398, 546, 587
AfiI CCNNNNNNNGG 4 cut(s) 385, 554, 1454, 2193
AflII CTTAAG 1 cut(s) 514
AflIII ACRYGT 1 cut(s) 491
AgsI TTSAA 8 cut(s) 37, 740, 1053, 1156, 1412, 1420, 1817, 2168
AhlI ACTAGT 1 cut(s) 469
AjnI CCWGG 4 cut(s) 378, 463, 2176, 2212
AleI CACNNNNGTG 2 cut(s) 300, 2215
Alw21I GWGCWC 1 cut(s) 858
Alw26I GTCTC 5 cut(s) 243, 374, 527, 659, 1225
AlwI GGATC 1 cut(s) 1439
Ama87I CYCGRG 1 cut(s) 420
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 7 cut(s) 259, 350, 440, 637, 685, 1102, 1294
ApeKI GCWGC 5 cut(s) 191, 222, 1458, 1735, 2018
ApoI RAATTY 2 cut(s) 1593, 1966
AseI ATTAAT 3 cut(s) 212, 842, 2096
AspLEI GCGC 2 cut(s) 118, 1626
AspS9I GGNCC 4 cut(s) 305, 557, 686, 2140
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 7 cut(s) 160, 397, 662, 1580, 1670, 2192, 2213
AvaI CYCGRG 1 cut(s) 420
AvaII GGWCC 3 cut(s) 305, 557, 2140
AxyI CCTNAGG 1 cut(s) 812
BaeGI GKGCMC 3 cut(s) 159, 1307, 1828
BalI TGGCCA 3 cut(s) 352, 442, 1104
BanI GGYRCC 1 cut(s) 1034
BanII GRGCYC 2 cut(s) 280, 858
BbrPI CACGTG 1 cut(s) 494
BbsI GAAGAC 2 cut(s) 394, 1743
Bbv12I GWGCWC 1 cut(s) 858
BbvI GCAGC 5 cut(s) 178, 234, 1470, 1747, 2005
BceAI ACGGC 2 cut(s) 624, 783
BcgI CGANNNNNNTGC 6 cut(s) 401, 435, 782, 816, 1815, 1849
BciT130I CCWGG 4 cut(s) 380, 465, 2178, 2214
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 5 cut(s) 243, 374, 527, 659, 1225
BcuI ACTAGT 1 cut(s) 469
BfaI CTAG 2 cut(s) 470, 2135
BfmI CTRYAG 2 cut(s) 1275, 1755
BfrI CTTAAG 1 cut(s) 514
BfuAI ACCTGC 1 cut(s) 85
BglI GCCNNNNNGGC 1 cut(s) 1302
BglII AGATCT 1 cut(s) 763
BisI GCNGC 6 cut(s) 192, 223, 259, 1459, 1736, 2019
BlsI GCNGC 6 cut(s) 193, 224, 260, 1460, 1737, 2020
BmcAI AGTACT 1 cut(s) 587
Bme1390I CCNGG 5 cut(s) 65, 380, 465, 2178, 2214
Bme18I GGWCC 3 cut(s) 305, 557, 2140
BmeT110I CYCGRG 1 cut(s) 420
BmgT120I GGNCC 4 cut(s) 305, 557, 686, 2140
BmiI GGNNCC 4 cut(s) 59, 279, 688, 1036
BmrFI CCNGG 5 cut(s) 65, 380, 465, 2178, 2214
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 6 cut(s) 78, 592, 1056, 1828, 1843, 1927
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 2 cut(s) 394, 1743
BplI GAGNNNNNCTC 4 cut(s) 575, 607, 1663, 1695
BpuEI CTTGAG 2 cut(s) 509, 577
BpuMI CCSGG 1 cut(s) 65
BsaAI YACGTR 1 cut(s) 494
BsaBI GATNNNNATC 2 cut(s) 606, 1070
BsaHI GRCGYC 1 cut(s) 1171
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 7 cut(s) 64, 463, 640, 747, 958, 1044, 2213
BsaWI WCCGGW 3 cut(s) 307, 547, 559
BsaXI ACNNNNNCTCC 2 cut(s) 2061, 2091
Bsc4I CCNNNNNNNGG 4 cut(s) 385, 554, 1454, 2193
Bse1I ACTGG 2 cut(s) 191, 559
Bse21I CCTNAGG 1 cut(s) 812
Bse3DI GCAATG 4 cut(s) 1608, 1657, 1681, 2026
Bse8I GATNNNNATC 2 cut(s) 606, 1070
BseAI TCCGGA 1 cut(s) 307
BseBI CCWGG 4 cut(s) 380, 465, 2178, 2214
BseDI CCNNGG 7 cut(s) 64, 463, 640, 747, 958, 1044, 2213
BseGI GGATG 2 cut(s) 586, 1843
BseJI GATNNNNATC 2 cut(s) 606, 1070
BseLI CCNNNNNNNGG 4 cut(s) 385, 554, 1454, 2193
BseMI GCAATG 4 cut(s) 1608, 1657, 1681, 2026
BseMII CTCAG 4 cut(s) 113, 843, 1820, 2048
BseNI ACTGG 2 cut(s) 191, 559
BseRI GAGGAG 2 cut(s) 580, 1859
BseSI GKGCMC 3 cut(s) 159, 1307, 1828
BseXI GCAGC 5 cut(s) 178, 234, 1470, 1747, 2005
BsgI GTGCAG 1 cut(s) 737
Bsh1236I CGCG 1 cut(s) 1380
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 7 cut(s) 261, 352, 442, 639, 687, 1104, 1296
BshNI GGYRCC 1 cut(s) 1034
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 858
BsiHKCI CYCGRG 1 cut(s) 420
BsiSI CCGG 4 cut(s) 64, 308, 548, 560
BsiWI CGTACG 1 cut(s) 396
BslI CCNNNNNNNGG 4 cut(s) 385, 554, 1454, 2193
BsmAI GTCTC 5 cut(s) 243, 374, 527, 659, 1225
BsmI GAATGC 2 cut(s) 901, 1262
BsnI GGCC 7 cut(s) 261, 352, 442, 639, 687, 1104, 1296
Bso31I GGTCTC 1 cut(s) 374
BsoBI CYCGRG 1 cut(s) 420
Bsp1286I GDGCHC 5 cut(s) 159, 280, 858, 1307, 1828
Bsp13I TCCGGA 1 cut(s) 307
Bsp143I GATC 5 cut(s) 150, 763, 1444, 1550, 1832
Bsp19I CCATGG 1 cut(s) 747
BspACI CCGC 6 cut(s) 12, 258, 787, 822, 1378, 1760
BspANI GGCC 7 cut(s) 261, 352, 442, 639, 687, 1104, 1296
BspCNI CTCAG 4 cut(s) 114, 844, 1821, 2049
BspEI TCCGGA 1 cut(s) 307
BspFNI CGCG 1 cut(s) 1380
BspLI GGNNCC 4 cut(s) 59, 279, 688, 1036
BspMI ACCTGC 1 cut(s) 85
BspPI GGATC 1 cut(s) 1439
BspQI GCTCTTC 1 cut(s) 1664
BspT107I GGYRCC 1 cut(s) 1034
BspTI CTTAAG 1 cut(s) 514
BspTNI GGTCTC 1 cut(s) 374
BsrDI GCAATG 4 cut(s) 1608, 1657, 1681, 2026
BsrI ACTGG 2 cut(s) 191, 559
BssECI CCNNGG 7 cut(s) 64, 463, 640, 747, 958, 1044, 2213
BssMI GATC 5 cut(s) 150, 763, 1444, 1550, 1832
BssNI GRCGYC 1 cut(s) 1171
BssT1I CCWWGG 3 cut(s) 747, 958, 1044
Bst2UI CCWGG 4 cut(s) 380, 465, 2178, 2214
Bst4CI ACNGT 6 cut(s) 242, 450, 946, 1090, 1279, 1395
Bst6I CTCTTC 6 cut(s) 92, 822, 1151, 1258, 1407, 1664
BstACI GRCGYC 1 cut(s) 1171
BstAFI CTTAAG 1 cut(s) 514
BstBAI YACGTR 1 cut(s) 494
BstC8I GCNNGC 4 cut(s) 180, 220, 485, 511
BstDEI CTNAG 6 cut(s) 122, 812, 852, 1726, 1829, 2057
BstDSI CCRYGG 2 cut(s) 640, 747
BstF5I GGATG 2 cut(s) 586, 1843
BstFNI CGCG 1 cut(s) 1380
BstHHI GCGC 2 cut(s) 118, 1626
BstKTI GATC 5 cut(s) 153, 766, 1447, 1553, 1835
BstMAI GTCTC 5 cut(s) 243, 374, 527, 659, 1225
BstMBI GATC 5 cut(s) 150, 763, 1444, 1550, 1832
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 6 cut(s) 188, 200, 275, 439, 830, 1302
BstNI CCWGG 4 cut(s) 380, 465, 2178, 2214
BstNSI RCATGY 1 cut(s) 1944
BstSCI CCNGG 5 cut(s) 63, 378, 463, 2176, 2212
BstSFI CTRYAG 2 cut(s) 1275, 1755
BstSLI GKGCMC 3 cut(s) 159, 1307, 1828
BstUI CGCG 1 cut(s) 1380
BstV1I GCAGC 5 cut(s) 178, 234, 1470, 1747, 2005
BstV2I GAAGAC 2 cut(s) 394, 1743
BstX2I RGATCY 1 cut(s) 763
BstXI CCANNNNNNTGG 1 cut(s) 1045
BstYI RGATCY 1 cut(s) 763
Bsu36I CCTNAGG 1 cut(s) 812
BsuRI GGCC 7 cut(s) 261, 352, 442, 639, 687, 1104, 1296
BtgI CCRYGG 2 cut(s) 640, 747
BtsCI GGATG 2 cut(s) 586, 1843
BtsIMutI CAGTG 3 cut(s) 446, 552, 1826
BveI ACCTGC 1 cut(s) 85
Cac8I GCNNGC 4 cut(s) 180, 220, 485, 511
CfoI GCGC 2 cut(s) 118, 1626
Cfr13I GGNCC 4 cut(s) 305, 557, 686, 2140
CseI GACGC 1 cut(s) 1160
CsiI ACCWGGT 1 cut(s) 2176
Csp6I GTAC 3 cut(s) 397, 545, 586
CviAII CATG 8 cut(s) 409, 577, 748, 893, 986, 1135, 1941, 2045
CviQI GTAC 3 cut(s) 397, 545, 586
DdeI CTNAG 6 cut(s) 122, 812, 852, 1726, 1829, 2057
DpnI GATC 5 cut(s) 152, 765, 1446, 1552, 1834
DpnII GATC 5 cut(s) 150, 763, 1444, 1550, 1832
DraIII CACNNNGTG 2 cut(s) 676, 2180
EaeI YGGCCR 3 cut(s) 350, 440, 1102
Eam1104I CTCTTC 6 cut(s) 92, 822, 1151, 1258, 1407, 1664
EarI CTCTTC 6 cut(s) 92, 822, 1151, 1258, 1407, 1664
Ecl136II GAGCTC 1 cut(s) 856
Eco130I CCWWGG 3 cut(s) 747, 958, 1044
Eco147I AGGCCT 1 cut(s) 1296
Eco24I GRGCYC 2 cut(s) 280, 858
Eco31I GGTCTC 1 cut(s) 374
Eco47I GGWCC 3 cut(s) 305, 557, 2140
Eco53kI GAGCTC 1 cut(s) 856
Eco57I CTGAAG 2 cut(s) 1514, 1982
Eco72I CACGTG 1 cut(s) 494
Eco81I CCTNAGG 1 cut(s) 812
Eco88I CYCGRG 1 cut(s) 420
EcoICRI GAGCTC 1 cut(s) 856
EcoO109I RGGNCCY 1 cut(s) 686
EcoRI GAATTC 1 cut(s) 1593
EcoRII CCWGG 4 cut(s) 378, 463, 2176, 2212
EcoT14I CCWWGG 3 cut(s) 747, 958, 1044
EcoT22I ATGCAT 1 cut(s) 1942
EcoT38I GRGCYC 2 cut(s) 280, 858
ErhI CCWWGG 3 cut(s) 747, 958, 1044
FaeI CATG 8 cut(s) 412, 580, 751, 896, 989, 1138, 1944, 2048
FalI AAGNNNNNCTT 2 cut(s) 1548, 1580
FatI CATG 8 cut(s) 408, 576, 747, 892, 985, 1134, 1940, 2044
FauI CCCGC 1 cut(s) 1753
FauNDI CATATG 1 cut(s) 2014
Fnu4HI GCNGC 6 cut(s) 192, 223, 259, 1459, 1736, 2019
FokI GGATG 2 cut(s) 593, 1850
FriOI GRGCYC 2 cut(s) 280, 858
Fsp4HI GCNGC 6 cut(s) 192, 223, 259, 1459, 1736, 2019
FspBI CTAG 2 cut(s) 470, 2135
GlaI GCGC 2 cut(s) 117, 1625
GluI GCNGC 6 cut(s) 192, 223, 259, 1459, 1736, 2019
HaeIII GGCC 7 cut(s) 261, 352, 442, 639, 687, 1104, 1296
HapII CCGG 4 cut(s) 64, 308, 548, 560
HgaI GACGC 1 cut(s) 1160
HhaI GCGC 2 cut(s) 118, 1626
Hin1I GRCGYC 1 cut(s) 1171
Hin1II CATG 8 cut(s) 412, 580, 751, 896, 989, 1138, 1944, 2048
Hin6I GCGC 2 cut(s) 116, 1624
HinP1I GCGC 2 cut(s) 116, 1624
HincII GTYRAC 1 cut(s) 417
HindII GTYRAC 1 cut(s) 417
HpaII CCGG 4 cut(s) 64, 308, 548, 560
HphI GGTGA 7 cut(s) 160, 397, 662, 1580, 1670, 2192, 2213
Hpy166II GTNNAC 1 cut(s) 417
Hpy188III TCNNGA 5 cut(s) 308, 422, 594, 949, 1685
Hpy8I GTNNAC 1 cut(s) 417
Hpy99I CGWCG 1 cut(s) 1593
HpyAV CCTTC 5 cut(s) 629, 677, 715, 1197, 1636
HpyCH4III ACNGT 6 cut(s) 242, 450, 946, 1090, 1279, 1395
HpyCH4IV ACGT 4 cut(s) 399, 493, 1226, 1335
HpyF10VI GCNNNNNNNGC 6 cut(s) 188, 200, 275, 439, 830, 1302
HpyF3I CTNAG 6 cut(s) 122, 812, 852, 1726, 1829, 2057
HpySE526I ACGT 4 cut(s) 399, 493, 1226, 1335
Hsp92I GRCGYC 1 cut(s) 1171
Hsp92II CATG 8 cut(s) 412, 580, 751, 896, 989, 1138, 1944, 2048
HspAI GCGC 2 cut(s) 116, 1624
Kpn2I TCCGGA 1 cut(s) 307
Kzo9I GATC 5 cut(s) 150, 763, 1444, 1550, 1832
LguI GCTCTTC 1 cut(s) 1664
LmnI GCTCC 2 cut(s) 283, 1957
Lsp1109I GCAGC 5 cut(s) 178, 234, 1470, 1747, 2005
LweI GCATC 6 cut(s) 78, 592, 1056, 1828, 1843, 1927
MabI ACCWGGT 1 cut(s) 2176
MaeI CTAG 2 cut(s) 470, 2135
MaeII ACGT 4 cut(s) 399, 493, 1226, 1335
MaeIII GTNAC 3 cut(s) 1012, 1576, 2077
MalI GATC 5 cut(s) 152, 765, 1446, 1552, 1834
MboI GATC 5 cut(s) 150, 763, 1444, 1550, 1832
MfeI CAATTG 1 cut(s) 2051
MflI RGATCY 1 cut(s) 763
MhlI GDGCHC 5 cut(s) 159, 280, 858, 1307, 1828
MlsI TGGCCA 3 cut(s) 352, 442, 1104
MluNI TGGCCA 3 cut(s) 352, 442, 1104
MlyI GAGTC 4 cut(s) 524, 661, 1368, 1376
MmeI TCCRAC 1 cut(s) 957
Mox20I TGGCCA 3 cut(s) 352, 442, 1104
Mph1103I ATGCAT 1 cut(s) 1942
MroI TCCGGA 1 cut(s) 307
MscI TGGCCA 3 cut(s) 352, 442, 1104
MslI CAYNNNNRTG 2 cut(s) 300, 2215
Msp20I TGGCCA 3 cut(s) 352, 442, 1104
MspA1I CMGCKG 2 cut(s) 650, 787
MspCI CTTAAG 1 cut(s) 514
MspI CCGG 4 cut(s) 64, 308, 548, 560
MspR9I CCNGG 5 cut(s) 65, 380, 465, 2178, 2214
MunI CAATTG 1 cut(s) 2051
Mva1269I GAATGC 2 cut(s) 901, 1262
MvaI CCWGG 4 cut(s) 380, 465, 2178, 2214
MvnI CGCG 1 cut(s) 1380
MwoI GCNNNNNNNGC 6 cut(s) 188, 200, 275, 439, 830, 1302
NciI CCSGG 1 cut(s) 65
NcoI CCATGG 1 cut(s) 747
NdeI CATATG 1 cut(s) 2014
NdeII GATC 5 cut(s) 150, 763, 1444, 1550, 1832
NlaIII CATG 8 cut(s) 412, 580, 751, 896, 989, 1138, 1944, 2048
NlaIV GGNNCC 4 cut(s) 59, 279, 688, 1036
NmeAIII GCCGAG 1 cut(s) 272
NmuCI GTSAC 1 cut(s) 2077
NsiI ATGCAT 1 cut(s) 1942
NspI RCATGY 1 cut(s) 1944
OliI CACNNNNGTG 2 cut(s) 300, 2215
PaeR7I CTCGAG 1 cut(s) 420
PceI AGGCCT 1 cut(s) 1296
PciSI GCTCTTC 1 cut(s) 1664
PctI GAATGC 2 cut(s) 901, 1262
PfeI GAWTC 6 cut(s) 653, 1049, 1214, 1320, 1489, 2183
Pfl23II CGTACG 1 cut(s) 396
PflFI GACNNNGTC 1 cut(s) 1702
PflMI CCANNNNNTGG 1 cut(s) 385
PkrI GCNGC 6 cut(s) 193, 224, 260, 1460, 1737, 2020
PleI GAGTC 4 cut(s) 524, 661, 1368, 1376
PmaCI CACGTG 1 cut(s) 494
PmlI CACGTG 1 cut(s) 494
PpsI GAGTC 4 cut(s) 524, 661, 1368, 1376
Ppu21I YACGTR 1 cut(s) 494
PshBI ATTAAT 3 cut(s) 212, 842, 2096
Psp124BI GAGCTC 1 cut(s) 858
Psp1406I AACGTT 1 cut(s) 1335
Psp6I CCWGG 4 cut(s) 378, 463, 2176, 2212
PspCI CACGTG 1 cut(s) 494
PspGI CCWGG 4 cut(s) 378, 463, 2176, 2212
PspLI CGTACG 1 cut(s) 396
PspN4I GGNNCC 4 cut(s) 59, 279, 688, 1036
PspPI GGNCC 4 cut(s) 305, 557, 686, 2140
PsuI RGATCY 1 cut(s) 763
PsyI GACNNNGTC 1 cut(s) 1702
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 3 cut(s) 398, 546, 587
RsaNI GTAC 3 cut(s) 397, 545, 586
RseI CAYNNNNRTG 2 cut(s) 300, 2215
SacI GAGCTC 1 cut(s) 858
SapI GCTCTTC 1 cut(s) 1664
SatI GCNGC 6 cut(s) 192, 223, 259, 1459, 1736, 2019
Sau3AI GATC 5 cut(s) 150, 763, 1444, 1550, 1832
Sau96I GGNCC 4 cut(s) 305, 557, 686, 2140
ScaI AGTACT 1 cut(s) 587
SchI GAGTC 4 cut(s) 524, 661, 1368, 1376
ScrFI CCNGG 5 cut(s) 65, 380, 465, 2178, 2214
SduI GDGCHC 5 cut(s) 159, 280, 858, 1307, 1828
SexAI ACCWGGT 1 cut(s) 2176
SfaNI GCATC 6 cut(s) 78, 592, 1056, 1828, 1843, 1927
SfcI CTRYAG 2 cut(s) 1275, 1755
Sfr274I CTCGAG 1 cut(s) 420
SinI GGWCC 3 cut(s) 305, 557, 2140
SlaI CTCGAG 1 cut(s) 420
SmiMI CAYNNNNRTG 2 cut(s) 300, 2215
SmlI CTYRAG 4 cut(s) 420, 514, 524, 592
SmoI CTYRAG 4 cut(s) 420, 514, 524, 592
SpeI ACTAGT 1 cut(s) 469
SseBI AGGCCT 1 cut(s) 1296
SsiI CCGC 6 cut(s) 12, 258, 787, 822, 1378, 1760
SspI AATATT 1 cut(s) 706
SspMI CTAG 2 cut(s) 470, 2135
SstI GAGCTC 1 cut(s) 858
StuI AGGCCT 1 cut(s) 1296
StyD4I CCNGG 5 cut(s) 63, 378, 463, 2176, 2212
StyI CCWWGG 3 cut(s) 747, 958, 1044
TaaI ACNGT 6 cut(s) 242, 450, 946, 1090, 1279, 1395
TaiI ACGT 4 cut(s) 402, 496, 1229, 1338
TaqI TCGA 3 cut(s) 421, 1064, 1684
TaqII GACCGA 1 cut(s) 1602
TatI WGTACW 1 cut(s) 585
TauI GCSGC 1 cut(s) 261
TfiI GAWTC 6 cut(s) 653, 1049, 1214, 1320, 1489, 2183
TscAI CASTG 3 cut(s) 453, 559, 1833
TseFI GTSAC 1 cut(s) 2077
TseI GCWGC 5 cut(s) 191, 222, 1458, 1735, 2018
Tsp45I GTSAC 1 cut(s) 2077
TspDTI ATGAA 6 cut(s) 608, 770, 885, 1225, 1367, 1393
TspRI CASTG 3 cut(s) 453, 559, 1833
Tth111I GACNNNGTC 1 cut(s) 1702
Van91I CCANNNNNTGG 1 cut(s) 385
Vha464I CTTAAG 1 cut(s) 514
VpaK11BI GGWCC 3 cut(s) 305, 557, 2140
VspI ATTAAT 3 cut(s) 212, 842, 2096
XapI RAATTY 2 cut(s) 1593, 1966
XceI RCATGY 1 cut(s) 1944
XcmI CCANNNNNNNNNTGG 2 cut(s) 638, 1132
XhoI CTCGAG 1 cut(s) 420
XspI CTAG 2 cut(s) 470, 2135
ZrmI AGTACT 1 cut(s) 587
Zsp2I ATGCAT 1 cut(s) 1942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.