Rorug03G0150200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000049
Physical Location & Seq
Forward (+)
12386942 .. 12389658
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0150200.1

Sequence Viewer

Length: 2214 bp
ATGTCGTCGGTGGCGCCACCCACTCTGGAGCTCCGAGAATTCAACCCCACACGACCCCGCGTCAACCCTACTCTCGCCGAGCTTCTCCAGTGGGTCGAACAGTCCCAAACCCCCGACCGGAAAACAACGTCGTCAAACCACAACAATGTCCACCACGTCCTCGACCTCGGGTTTCCTTCTACCTCTTTATCTCAGTCGACCTACCCTTTCGTCCTCTCCTTCAACAATCTCACTTACAGCGTCAAGGTCCGGCGCAAAATGACATTACTGCCCTCATTCAGATCAGCAGAGGCTAAACTTGGGGGTAGCCCAAGCAGCAGCAGCACCAAGGTTTTGCTGAACGGCGTCTCCGGCGAGGCCAGAGAAGGCCAGATCATGGCGGTTCTCGGAGCGAGTGGCTCCGGCAAGTCGACGCTGATAGACGCATTGGCTGATCGGATCGCCAAGTACAGCTTGAAAGGGACGGTCACATTAAACGATGAGATTCTCAACTCGAGGATGCTGAAGGTGATATCGGCTTACGTCATGCAGGACGATCTCTTGTTTCCGATGCTGACGGTGGAGGAGACTCTCATGTTCTCCGCCGAGTTCCGGCTCCCCCGGACTCTGTCGAAATCCAAGAAGAAGTCTCGTGTTCAGGCCCTTATTGATCAATTAGGTCTCCGGAACGCCGCGAAGACGGTGATCGGAGATGAGGGCCACCGCGGCGTCTCCGGCGGGGAGAGGAGGAGAGTTTCTATTGGTATTGACATCATTCACGACCCGATAGTTTTGTTTCTGGACGAGCCGACCTCCGGGCTTGACTCGACAAGCGCTTTCATGGTGGTGAAGGTTTTGCAGAGAATAGCTCAGAGTGGTAGTATTGTGGTGATGTCCGTACATCAACCCAGTTACAGGATTTTGGGGTTGCTGGACCGGTTGGTGCTTCTATCACATGGACAGACAGTGTACAGTGGCTCGCCGGCGAATCTTCCAATCTTTTTCGGCGAATTCGGACACCCTATACCCGAAACGGAGAACAGGACCGAGTTCGCTCTGGACCTCATTCGAGAATTGGAGGAAACTCCAGGTGGAACCAAGAGCTTGGTGGAGTTCAACAAGTCATGGCAAGTCACCAAGAGGCAAAAGCAGAAAGACGCAAATGGTTGCGACAACGGTGCCTGCAGTATGCCGAAGCTCTCGCTCAAAGATGCCATAAGTGCAAGCATTTCAAGAGGAAAGCTAGTCTCGGGTGCTCCCACTGACACTAACCTAGCCTCTTCAGTCCCTACATTTGCCAACCCGATGTGGATCGAAATGTTGGTGATCTCGAAACGCTCGGTCACAAACTCGAGACGGATGCCGGAACTCTTCGGCATCCGATTGGGGGCGGTACTTGTCACCGGAATAATATTAGCCACTATGTTTTGGCACCTCGACGACTCCCCCATGGGTGTTGAAGAGCGCCTTGGATTTTTTGCCTTTGCAATGTCCACGACCTTTTACACTTGCGCTGAAGCAATCCCTATTTTCCTCCAAGAACGATACATATTCATGAGAGAAACAGCCTACAATGCATACCGTCGGTCATCTTATGTTTTAGCTCACTCGATACTCTCCATTCCCTCCTTGCTCATCCTCTCTTTGGCCTTTGCCGCCACGACGTTTTGGGCCGTGGGGTTGGCCGGTGGCCTTCATGGGTTTCTCTTTTTCTTCTTGACAATATGCTCAGCATTTTGGGCAGGATCTTCGTTCGTAACGTTTTTATCTGGTGTTGTGACCCATGTCATGGTGGGGTTCACGGTTGTGGTGGCCATTTTAGCATACTTTCTCCTCTTCAGTGGGTTTTTCATTAGCAGAGATCGAATTCCGGTTTACTGGATTTGGTTCCATTACATTTCTCTGGTGAAATATCCATATGAAGCTGTTTTGCAGAACGAGTTTGATGACCCGATGAAGTGTTTAGTGACCGGAATTCAAATGTTCGACAATACGCCGTTGGGTGAGGTCCCTGTTGCATTGAAGTTGAAGTTGTTAAAGAACATGAGCAACATTTTAGGGTTGAATATAACTGGGTCTAGTTGTGTGACCACTGGATTAGACATTTTGAAGCAGCAGGGTATAACCGATATTAGTAAATGGAATTGCTTATGGGTAACAATTGCTTGGGGCTTTTTTTTTAGAATTTTGTTCTATTTTACTTTGTTGTTTGGGAGCAGAAATAAAAGGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

737

Amino Acids

81.7

Weight (kDa)

9.25

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 113 - 265 6.4e-26 ABC transporter
ABC2_membrane PF01061 434 - 641 9.5e-46 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000049)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g09850 FvH4_2g12512 FvH4_2g19830 FvH4_2g21281 FvH4_2g30300 FvH4_3g04012 FvH4_3g12601 FvH4_3g25620 FvH4_3g28881 FvH4_3g29932 FvH4_4g12651 FvH4_5g27100 FvH4_6g03011 FvH4_6g10710 FvH4_6g10711 FvH4_6g19991 FvH4_6g25461 FvH4_6g32661 FvH4_7g16371
rosa_chinensis RchiOBHm_Chr1g0347111 RchiOBHm_Chr1g0350781 RchiOBHm_Chr1g0350971 RchiOBHm_Chr2g0123951 RchiOBHm_Chr4g0397591 RchiOBHm_Chr5g0032121 RchiOBHm_Chr6g0265951
rosa_laevigata RLG00000000709 RLG00000001093 RLG00000001244 RLG00000002381 RLG00000003581 RLG00000003584 RLG00000004011 RLG00000004587 RLG00000005734 RLG00000007804 RLG00000008145 RLG00000008422 RLG00000008949 RLG00000009240 RLG00000009334 RLG00000009773 RLG00000010531 RLG00000012000 RLG00000012913 RLG00000013188 RLG00000013289 RLG00000013302 RLG00000013943 RLG00000016032 RLG00000016277 RLG00000016479 RLG00000017591 RLG00000017899 RLG00000018452 RLG00000018455 RLG00000019343 RLG00000021649 RLG00000023222 RLG00000023677 RLG00000024058 RLG00000024433 RLG00000026719 RLG00000027778 RLG00000028367 RLG00000028761 RLG00000029079 RLG00000030238 RLG00000030452 RLG00000030719 RLG00000030834 RLG00000031739 RLG00000032099 RLG00000033644 RLG00000033713 RLG00000034081 RLG00000035108 RLG00000035250 RLG00000035711 RLG00000036556 RLG00000036747
rosa_multiflora Rmu_co8042282.1_g000001 Rmu_co8251111.1_g000001 Rmu_co8268445.1_g000001 Rmu_co8273215.1_g000001 Rmu_co8469153.1_g000002 Rmu_sc0000035.1_g000023 Rmu_sc0000092.1_g000002 Rmu_sc0000166.1_g000008 Rmu_sc0000260.1_g000017 Rmu_sc0000317.1_g000014 Rmu_sc0000470.1_g000023 Rmu_sc0000533.1_g000060 Rmu_sc0000539.1_g000011 Rmu_sc0000546.1_g000022 Rmu_sc0000571.1_g000022 Rmu_sc0000610.1_g000019 Rmu_sc0000706.1_g000038 Rmu_sc0000843.1_g000005 Rmu_sc0000882.1_g000008 Rmu_sc0000928.1_g000014 Rmu_sc0000928.1_g000015 Rmu_sc0001140.1_g000009 Rmu_sc0001161.1_g000015 Rmu_sc0001179.1_g000041 Rmu_sc0001296.1_g000014 Rmu_sc0001373.1_g000023 Rmu_sc0001433.1_g000015 Rmu_sc0001519.1_g000009 Rmu_sc0001878.1_g000011 Rmu_sc0002112.1_g000014 Rmu_sc0002141.1_g000030 Rmu_sc0002148.1_g000029 Rmu_sc0002156.1_g000014 Rmu_sc0002199.1_g000007 Rmu_sc0002586.1_g000033 Rmu_sc0002620.1_g000008 Rmu_sc0002646.1_g000010 Rmu_sc0002800.1_g000026 Rmu_sc0002921.1_g000001 Rmu_sc0002964.1_g000009 Rmu_sc0002989.1_g000009 Rmu_sc0003133.1_g000002 Rmu_sc0003238.1_g000030 Rmu_sc0003314.1_g000011 Rmu_sc0003314.1_g000012 Rmu_sc0003314.1_g000028 Rmu_sc0003458.1_g000003 Rmu_sc0003499.1_g000034 Rmu_sc0003610.1_g000040 Rmu_sc0003859.1_g000006 Rmu_sc0003971.1_g000002 Rmu_sc0004161.1_g000006 Rmu_sc0004250.1_g000023 Rmu_sc0004467.1_g000071 Rmu_sc0004498.1_g000004 Rmu_sc0004521.1_g000024 Rmu_sc0004529.1_g000030 Rmu_sc0004547.1_g000009 Rmu_sc0004835.1_g000034 Rmu_sc0004944.1_g000012 Rmu_sc0005030.1_g000005 Rmu_sc0005030.1_g000006 Rmu_sc0005137.1_g000033 Rmu_sc0005294.1_g000013 Rmu_sc0005416.1_g000020 Rmu_sc0005426.1_g000010 Rmu_sc0005432.1_g000001 Rmu_sc0005450.1_g000020 Rmu_sc0005566.1_g000008 Rmu_sc0005693.1_g000020 Rmu_sc0005992.1_g000007 Rmu_sc0006007.1_g000001 Rmu_sc0006016.1_g000017 Rmu_sc0006016.1_g000018 Rmu_sc0006147.1_g000009 Rmu_sc0006536.1_g000032 Rmu_sc0006536.1_g000033 Rmu_sc0006572.1_g000006 Rmu_sc0007474.1_g000013 Rmu_sc0007490.1_g000008 Rmu_sc0007490.1_g000009 Rmu_sc0007618.1_g000003 Rmu_sc0007753.1_g000021 Rmu_sc0008039.1_g000001 Rmu_sc0008044.1_g000008 Rmu_sc0008155.1_g000003 Rmu_sc0008308.1_g000006 Rmu_sc0008308.1_g000017 Rmu_sc0008360.1_g000006 Rmu_sc0008380.1_g000015 Rmu_sc0008596.1_g000002 Rmu_sc0008596.1_g000003 Rmu_sc0008629.1_g000013 Rmu_sc0008991.1_g000011 Rmu_sc0009015.1_g000004 Rmu_sc0009015.1_g000005 Rmu_sc0009360.1_g000050 Rmu_sc0009386.1_g000009 Rmu_sc0009410.1_g000003 Rmu_sc0009410.1_g000006 Rmu_sc0009609.1_g000001 Rmu_sc0010317.1_g000003 Rmu_sc0010871.1_g000016 Rmu_sc0010999.1_g000018 Rmu_sc0011019.1_g000009 Rmu_sc0011343.1_g000014 Rmu_sc0011578.1_g000019 Rmu_sc0011781.1_g000012 Rmu_sc0011921.1_g000008 Rmu_sc0012005.1_g000001 Rmu_sc0012179.1_g000006 Rmu_sc0012211.1_g000007 Rmu_sc0012220.1_g000002 Rmu_sc0012742.1_g000001 Rmu_sc0013620.1_g000004 Rmu_sc0014003.1_g000007 Rmu_sc0014203.1_g000024 Rmu_sc0014612.1_g000004 Rmu_sc0014667.1_g000006 Rmu_sc0015359.1_g000002 Rmu_sc0016526.1_g000003 Rmu_sc0017253.1_g000008 Rmu_sc0017394.1_g000005 Rmu_sc0017394.1_g000006 Rmu_sc0018840.1_g000001 Rmu_sc0019315.1_g000001 Rmu_sc0022276.1_g000002 Rmu_sc0024289.1_g000001 Rmu_sc0024918.1_g000004 Rmu_sc0026424.1_g000001 Rmu_sc0026425.1_g000001 Rmu_sc0030616.1_g000001 Rmu_sc0030617.1_g000001 Rmu_sc0031931.1_g000003 Rmu_sc0034665.1_g000001 Rmu_sc0035393.1_g000001 Rmu_ssc0000162.1_g000015 Rmu_ssc0000255.1_g000060 Rmu_ssc0000409.1_g000012
rosa_roxburghii Rroxscaffold_1G00006160 Rroxscaffold_2G00084140 Rroxscaffold_2G00109070 Rroxscaffold_2G00136190 Rroxscaffold_2G00136200 Rroxscaffold_3G00237880 Rroxscaffold_3G00250500 Rroxscaffold_3G00272860 Rroxscaffold_4G00283710 Rroxscaffold_4G00308190 Rroxscaffold_5G00334250 Rroxscaffold_5G00339840 Rroxscaffold_5G00380790 Rroxscaffold_7G00177140 Rroxscaffold_7G00186880 Rroxscaffold_7G00201080
rosa_rugosa Rorug01G0002600 Rorug01G0159800.1 Rorug01G0164900 Rorug01G0176500 Rorug01G0185400 Rorug01G0223500 Rorug01G0231900 Rorug01G0251700 Rorug01G0283600 Rorug01G0309600 Rorug01G0333600 Rorug01G0478500 Rorug01G0478700 Rorug01G0483600 Rorug02G0000400 Rorug02G0025400 Rorug02G0025500 Rorug02G0160600 Rorug02G0179800 Rorug02G0221000 Rorug02G0222000 Rorug02G0239900 Rorug02G0291000 Rorug02G0292800 Rorug02G0299400 Rorug02G0361300 Rorug02G0382000 Rorug02G0438400 Rorug02G0438400 Rorug02G0463000 Rorug02G0599300 Rorug03G0085500 Rorug03G0143300 Rorug03G0150200 Rorug03G0214500 Rorug03G0301800 Rorug03G0304100 Rorug03G0304200 Rorug03G0318900 Rorug03G0342400 Rorug03G0363300.1 Rorug04G0006400 Rorug04G0026200 Rorug04G0185500 Rorug04G0185600 Rorug04G0242600 Rorug04G0294300 Rorug04G0322000 Rorug04G0380900 Rorug05G0050300 Rorug05G0050300 Rorug05G0086800 Rorug05G0106900 Rorug05G0166700 Rorug05G0220300 Rorug05G0237400 Rorug05G0296300 Rorug05G0313400 Rorug05G0323000 Rorug05G0347200 Rorug05G0363800 Rorug05G0413500 Rorug05G0440900 Rorug05G0498500 Rorug05G0576300 Rorug06G0025600 Rorug06G0029800 Rorug06G0045700 Rorug06G0087400 Rorug06G0087600 Rorug06G0108200 Rorug06G0110200 Rorug06G0129900 Rorug06G0129900 Rorug06G0133800 Rorug06G0141200 Rorug06G0219200 Rorug06G0268900 Rorug06G0269000 Rorug06G0291100 Rorug06G0479100 Rorug06G0479200 Rorug06G0511800 Rorug07G0028800.1 Rorug07G0052500 Rorug07G0071900 Rorug07G0076600 Rorug07G0079900 Rorug07G0085200 Rorug07G0302400 Rorug07G0303700 Rorug07G0318000
rosa_samantha Rh1AG194500 Rh1AG194600 Rh1AG354700 Rh1BG147200 Rh1BG169000 Rh1BG212500 Rh1BG241800 Rh1CG179300 Rh2AG232000 Rh2BG307200 Rh2BG352400 Rh2BG563400 Rh2CG200100 Rh2CG285300 Rh2CG331100 Rh2CG533700 Rh2CG574800 Rh2CG650600 Rh2CG650700 Rh2DG212200 Rh3BG142200 Rh3BG294100 Rh3BG300500 Rh3CG143500 Rh3DG143400 Rh4AG079100 Rh4AG125800 Rh4BG119600 Rh4BG187000 Rh4BG248400 Rh4CG036500 Rh4CG084700 Rh4CG134000 Rh4CG198900 Rh5AG384400 Rh5BG212700 Rh5CG235900 Rh5CG292600 Rh5CG401000 Rh5CG502100 Rh5CG506700 Rh5DG020900 Rh5DG269500 Rh5DG330300 Rh5DG388800 Rh6AG031600 Rh6AG257800 Rh6AG375600 Rh6BG338400 Rh6BG418300 Rh6CG280900 Rh6DG411200 Rh7AG075900 Rh7BG073500 Rh7BG088700 Rh7BG210200 Rh7BG260400 Rh7BG269600 Rh7BG270600 Rh7BG459100 Rh7BG459200 Rh7CG258100
rosa_wichuraiana Rw0G014160 Rw0G019160 Rw1G007800 Rw1G013530 Rw1G036200 Rw2G016380 Rw2G022170 Rw2G036380 Rw3G001880 Rw4G026250 Rw5G017280 Rw5G045990 Rw6G019190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 13, 1157, 1410
AccB7I CCANNNNNTGG 2 cut(s) 376, 1768
AccI GTMKAC 2 cut(s) 197, 410
AccII CGCG 3 cut(s) 60, 674, 705
AccIII TCCGGA 1 cut(s) 663
AciI CCGC 9 cut(s) 58, 380, 582, 672, 703, 705, 717, 1370, 1635
AclI AACGTT 1 cut(s) 1739
AclWI GGATC 3 cut(s) 446, 1298, 1732
AcoI YGGCCR 2 cut(s) 1662, 1791
AcsI RAATTY 5 cut(s) 38, 989, 1845, 1953, 2163
AcuI CTGAAG 4 cut(s) 524, 1245, 1515, 1801
AcyI GRCGYC 3 cut(s) 14, 345, 708
AfaI GTAC 4 cut(s) 449, 879, 950, 1374
AfeI AGCGCT 1 cut(s) 814
AfiI CCNNNNNNNGG 8 cut(s) 117, 376, 591, 794, 894, 1366, 1624, 1768
AgeI ACCGGT 1 cut(s) 915
AhdI GACNNNNNGTC 1 cut(s) 59
AjiI CACGTC 1 cut(s) 157
AjnI CCWGG 1 cut(s) 1066
AjuI GAANNNNNNNTTGG 2 cut(s) 1431, 1463
AleI CACNNNNGTG 1 cut(s) 1784
AloI GAACNNNNNNTCC 2 cut(s) 332, 364
AluBI AGCT 9 cut(s) 31, 82, 453, 848, 1083, 1177, 1222, 1583, 1904
AluI AGCT 9 cut(s) 31, 82, 453, 848, 1083, 1177, 1222, 1583, 1904
Alw21I GWGCWC 2 cut(s) 33, 1237
Alw26I GTCTC 7 cut(s) 352, 560, 633, 665, 715, 1231, 1327
AlwI GGATC 3 cut(s) 446, 1298, 1732
Ama87I CYCGRG 4 cut(s) 167, 493, 1228, 1330
Aor13HI TCCGGA 1 cut(s) 663
Aor51HI AGCGCT 1 cut(s) 814
AoxI GGCC 9 cut(s) 357, 367, 639, 697, 1626, 1650, 1662, 1669, 1791
ApeKI GCWGC 4 cut(s) 315, 318, 321, 2092
ApoI RAATTY 5 cut(s) 38, 989, 1845, 1953, 2163
AsiGI ACCGGT 1 cut(s) 915
AspLEI GCGC 5 cut(s) 16, 255, 815, 1446, 1493
AspS9I GGNCC 8 cut(s) 247, 640, 697, 913, 1023, 1039, 1650, 1987
AsuC2I CCSGG 2 cut(s) 601, 796
AsuHPI GGTGA 9 cut(s) 520, 694, 838, 880, 1105, 1315, 1372, 1897, 1994
AvaI CYCGRG 4 cut(s) 167, 493, 1228, 1330
AvaII GGWCC 5 cut(s) 247, 913, 1023, 1039, 1987
BalI TGGCCA 1 cut(s) 1793
BanI GGYRCC 3 cut(s) 13, 1157, 1410
BanII GRGCYC 1 cut(s) 33
BauI CACGAG 1 cut(s) 630
BbsI GAAGAC 1 cut(s) 683
Bbv12I GWGCWC 2 cut(s) 33, 1237
BbvI GCAGC 4 cut(s) 327, 330, 333, 2104
BceAI ACGGC 3 cut(s) 358, 1637, 1960
BcgI CGANNNNNNTGC 6 cut(s) 1139, 1173, 1321, 1355, 1710, 1744
BciT130I CCWGG 1 cut(s) 1068
BclI TGATCA 1 cut(s) 649
BcnI CCSGG 2 cut(s) 601, 796
BcoDI GTCTC 7 cut(s) 352, 560, 633, 665, 715, 1231, 1327
BfaI CTAG 3 cut(s) 1223, 1253, 2058
BfmI CTRYAG 1 cut(s) 1162
BfoI RGCGCY 3 cut(s) 17, 816, 1447
BglI GCCNNNNNGGC 1 cut(s) 705
BisI GCNGC 7 cut(s) 316, 319, 322, 672, 706, 1635, 2093
BlpI GCTNAGC 1 cut(s) 1708
BlsI GCNGC 7 cut(s) 317, 320, 323, 673, 707, 1636, 2094
Bme1390I CCNGG 3 cut(s) 601, 796, 1068
Bme18I GGWCC 5 cut(s) 247, 913, 1023, 1039, 1987
BmeRI GACNNNNNGTC 1 cut(s) 59
BmeT110I CYCGRG 4 cut(s) 167, 493, 1228, 1330
BmgBI CACGTC 1 cut(s) 157
BmgT120I GGNCC 8 cut(s) 247, 640, 697, 913, 1023, 1039, 1650, 1987
BmiI GGNNCC 8 cut(s) 15, 400, 596, 1075, 1159, 1412, 1868, 1989
BmrFI CCNGG 3 cut(s) 601, 796, 1068
BmrI ACTGGG 2 cut(s) 882, 2061
BmsI GCATC 5 cut(s) 489, 540, 1180, 1329, 1365
BmuI ACTGGG 2 cut(s) 882, 2061
BoxI GACNNNNGTC 1 cut(s) 1763
BpiI GAAGAC 1 cut(s) 683
BplI GAGNNNNNCTC 4 cut(s) 776, 808, 832, 864
BpmI CTGGAG 3 cut(s) 47, 71, 1050
Bpu1102I GCTNAGC 1 cut(s) 1708
BpuMI CCSGG 2 cut(s) 601, 796
BsaBI GATNNNNATC 1 cut(s) 1289
BsaHI GRCGYC 3 cut(s) 14, 345, 708
BsaI GGTCTC 1 cut(s) 665
BsaJI CCNNGG 7 cut(s) 166, 327, 599, 703, 1428, 1447, 1653
BsaWI WCCGGW 6 cut(s) 117, 663, 915, 1382, 1849, 1949
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 8 cut(s) 117, 376, 591, 794, 894, 1366, 1624, 1768
Bse118I RCCGGY 3 cut(s) 915, 961, 1664
Bse1I ACTGG 5 cut(s) 88, 888, 1862, 2056, 2077
Bse3DI GCAATG 1 cut(s) 1473
Bse8I GATNNNNATC 1 cut(s) 1289
BseAI TCCGGA 1 cut(s) 663
BseBI CCWGG 1 cut(s) 1068
BseDI CCNNGG 7 cut(s) 166, 327, 599, 703, 1428, 1447, 1653
BseGI GGATG 4 cut(s) 504, 1344, 1356, 1614
BseJI GATNNNNATC 1 cut(s) 1289
BseLI CCNNNNNNNGG 8 cut(s) 117, 376, 591, 794, 894, 1366, 1624, 1768
BseMI GCAATG 1 cut(s) 1473
BseMII CTCAG 3 cut(s) 206, 863, 1722
BseNI ACTGG 5 cut(s) 88, 888, 1862, 2056, 2077
BseRI GAGGAG 4 cut(s) 578, 739, 742, 1802
BseXI GCAGC 4 cut(s) 327, 330, 333, 2104
Bsh1236I CGCG 3 cut(s) 60, 674, 705
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 9 cut(s) 359, 369, 641, 699, 1628, 1652, 1664, 1671, 1793
BshNI GGYRCC 3 cut(s) 13, 1157, 1410
BshTI ACCGGT 1 cut(s) 915
BsiEI CGRYCG 1 cut(s) 118
BsiHKAI GWGCWC 2 cut(s) 33, 1237
BsiHKCI CYCGRG 4 cut(s) 167, 493, 1228, 1330
BslFI GGGAC 4 cut(s) 88, 475, 1250, 1973
BslI CCNNNNNNNGG 8 cut(s) 117, 376, 591, 794, 894, 1366, 1624, 1768
BsmAI GTCTC 7 cut(s) 352, 560, 633, 665, 715, 1231, 1327
BsmBI CGTCTC 3 cut(s) 352, 715, 1327
BsmFI GGGAC 4 cut(s) 88, 475, 1250, 1973
BsnI GGCC 9 cut(s) 359, 369, 641, 699, 1628, 1652, 1664, 1671, 1793
Bso31I GGTCTC 1 cut(s) 665
BsoBI CYCGRG 4 cut(s) 167, 493, 1228, 1330
Bsp1286I GDGCHC 2 cut(s) 33, 1237
Bsp13I TCCGGA 1 cut(s) 663
Bsp1407I TGTACA 1 cut(s) 948
Bsp1720I GCTNAGC 1 cut(s) 1708
Bsp19I CCATGG 1 cut(s) 1428
BspACI CCGC 9 cut(s) 58, 380, 582, 672, 703, 705, 717, 1370, 1635
BspANI GGCC 9 cut(s) 359, 369, 641, 699, 1628, 1652, 1664, 1671, 1793
BspCNI CTCAG 3 cut(s) 205, 862, 1721
BspEI TCCGGA 1 cut(s) 663
BspFNI CGCG 3 cut(s) 60, 674, 705
BspHI TCATGA 1 cut(s) 1533
BspLI GGNNCC 8 cut(s) 15, 400, 596, 1075, 1159, 1412, 1868, 1989
BspMAI CTGCAG 1 cut(s) 1166
BspPI GGATC 3 cut(s) 446, 1298, 1732
BspQI GCTCTTC 1 cut(s) 1434
BspT107I GGYRCC 3 cut(s) 13, 1157, 1410
BspTNI GGTCTC 1 cut(s) 665
BsrDI GCAATG 1 cut(s) 1473
BsrFI RCCGGY 3 cut(s) 915, 961, 1664
BsrGI TGTACA 1 cut(s) 948
BsrI ACTGG 5 cut(s) 88, 888, 1862, 2056, 2077
BssAI RCCGGY 3 cut(s) 915, 961, 1664
BssECI CCNNGG 7 cut(s) 166, 327, 599, 703, 1428, 1447, 1653
BssNI GRCGYC 3 cut(s) 14, 345, 708
BssSI CACGAG 1 cut(s) 630
BssT1I CCWWGG 3 cut(s) 327, 1428, 1447
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 1 cut(s) 1068
Bst4CI ACNGT 9 cut(s) 102, 466, 559, 682, 946, 953, 1157, 1562, 1783
Bst6I CTCTTC 4 cut(s) 1264, 1355, 1434, 1820
BstACI GRCGYC 3 cut(s) 14, 345, 708
BstAUI TGTACA 1 cut(s) 948
BstC8I GCNNGC 4 cut(s) 959, 963, 1162, 1204
BstDEI CTNAG 3 cut(s) 192, 849, 1708
BstDSI CCRYGG 3 cut(s) 703, 1428, 1653
BstF5I GGATG 4 cut(s) 504, 1344, 1356, 1614
BstFNI CGCG 3 cut(s) 60, 674, 705
BstH2I RGCGCY 3 cut(s) 17, 816, 1447
BstHHI GCGC 5 cut(s) 16, 255, 815, 1446, 1493
BstMAI GTCTC 7 cut(s) 352, 560, 633, 665, 715, 1231, 1327
BstMCI CGRYCG 1 cut(s) 118
BstNI CCWGG 1 cut(s) 1068
BstPAI GACNNNNGTC 1 cut(s) 1763
BstSCI CCNGG 3 cut(s) 599, 794, 1066
BstSFI CTRYAG 1 cut(s) 1162
BstUI CGCG 3 cut(s) 60, 674, 705
BstV1I GCAGC 4 cut(s) 327, 330, 333, 2104
BstV2I GAAGAC 1 cut(s) 683
BstX2I RGATCY 1 cut(s) 1724
BstXI CCANNNNNNTGG 1 cut(s) 1084
BstYI RGATCY 1 cut(s) 1724
BsuRI GGCC 9 cut(s) 359, 369, 641, 699, 1628, 1652, 1664, 1671, 1793
BtgI CCRYGG 3 cut(s) 703, 1428, 1653
BtrI CACGTC 1 cut(s) 157
BtsCI GGATG 4 cut(s) 504, 1344, 1356, 1614
BtsIMutI CAGTG 6 cut(s) 95, 951, 958, 1239, 1825, 2070
Cac8I GCNNGC 4 cut(s) 959, 963, 1162, 1204
CciI TCATGA 1 cut(s) 1533
CfoI GCGC 5 cut(s) 16, 255, 815, 1446, 1493
Cfr10I RCCGGY 3 cut(s) 915, 961, 1664
Cfr13I GGNCC 8 cut(s) 247, 640, 697, 913, 1023, 1039, 1650, 1987
Cfr42I CCGCGG 1 cut(s) 706
CseI GACGC 7 cut(s) 49, 229, 334, 421, 431, 697, 1145
Csp6I GTAC 4 cut(s) 448, 878, 949, 1373
CspAI ACCGGT 1 cut(s) 915
CviQI GTAC 4 cut(s) 448, 878, 949, 1373
DdeI CTNAG 3 cut(s) 192, 849, 1708
DinI GGCGCC 1 cut(s) 15
DriI GACNNNNNGTC 1 cut(s) 59
EaeI YGGCCR 2 cut(s) 1662, 1791
Eam1104I CTCTTC 4 cut(s) 1264, 1355, 1434, 1820
Eam1105I GACNNNNNGTC 1 cut(s) 59
EarI CTCTTC 4 cut(s) 1264, 1355, 1434, 1820
EciI GGCGGA 1 cut(s) 571
Ecl136II GAGCTC 1 cut(s) 31
Eco130I CCWWGG 3 cut(s) 327, 1428, 1447
Eco24I GRGCYC 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 665
Eco32I GATATC 1 cut(s) 513
Eco47I GGWCC 5 cut(s) 247, 913, 1023, 1039, 1987
Eco47III AGCGCT 1 cut(s) 814
Eco53kI GAGCTC 1 cut(s) 31
Eco57I CTGAAG 4 cut(s) 524, 1245, 1515, 1801
Eco88I CYCGRG 4 cut(s) 167, 493, 1228, 1330
EcoICRI GAGCTC 1 cut(s) 31
EcoO109I RGGNCCY 2 cut(s) 640, 1987
EcoRI GAATTC 4 cut(s) 38, 989, 1845, 1953
EcoRII CCWGG 1 cut(s) 1066
EcoRV GATATC 1 cut(s) 513
EcoT14I CCWWGG 3 cut(s) 327, 1428, 1447
EcoT22I ATGCAT 1 cut(s) 1558
EcoT38I GRGCYC 1 cut(s) 33
EgeI GGCGCC 1 cut(s) 15
EheI GGCGCC 1 cut(s) 15
ErhI CCWWGG 3 cut(s) 327, 1428, 1447
Esp3I CGTCTC 3 cut(s) 352, 715, 1327
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 1431, 1463
FaqI GGGAC 4 cut(s) 88, 475, 1250, 1973
FauI CCCGC 2 cut(s) 65, 710
FauNDI CATATG 1 cut(s) 1897
FbaI TGATCA 1 cut(s) 649
FblI GTMKAC 2 cut(s) 197, 410
Fnu4HI GCNGC 7 cut(s) 316, 319, 322, 672, 706, 1635, 2093
FokI GGATG 4 cut(s) 511, 1343, 1351, 1601
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 7 cut(s) 316, 319, 322, 672, 706, 1635, 2093
FspBI CTAG 3 cut(s) 1223, 1253, 2058
GlaI GCGC 5 cut(s) 15, 254, 814, 1445, 1492
GluI GCNGC 7 cut(s) 316, 319, 322, 672, 706, 1635, 2093
GsuI CTGGAG 3 cut(s) 47, 71, 1050
HaeII RGCGCY 3 cut(s) 17, 816, 1447
HaeIII GGCC 9 cut(s) 359, 369, 641, 699, 1628, 1652, 1664, 1671, 1793
HgaI GACGC 7 cut(s) 49, 229, 334, 421, 431, 697, 1145
HhaI GCGC 5 cut(s) 16, 255, 815, 1446, 1493
Hin1I GRCGYC 3 cut(s) 14, 345, 708
Hin6I GCGC 5 cut(s) 14, 253, 813, 1444, 1491
HinP1I GCGC 5 cut(s) 14, 253, 813, 1444, 1491
HincII GTYRAC 3 cut(s) 64, 198, 411
HindII GTYRAC 3 cut(s) 64, 198, 411
HinfI GANTC 6 cut(s) 484, 568, 604, 803, 967, 1421
HphI GGTGA 9 cut(s) 520, 694, 838, 880, 1105, 1315, 1372, 1897, 1994
Hpy166II GTNNAC 8 cut(s) 64, 151, 198, 411, 949, 1473, 1779, 1855
Hpy188I TCNGA 9 cut(s) 35, 281, 389, 438, 549, 689, 852, 995, 1361
Hpy8I GTNNAC 8 cut(s) 64, 151, 198, 411, 949, 1473, 1779, 1855
Hpy99I CGWCG 6 cut(s) 10, 133, 415, 1421, 1566, 1645
HpyAV CCTTC 6 cut(s) 186, 229, 359, 499, 823, 1682
HpyCH4III ACNGT 9 cut(s) 102, 466, 559, 682, 946, 953, 1157, 1562, 1783
HpyCH4IV ACGT 5 cut(s) 128, 156, 522, 1643, 1739
HpyCH4V TGCA 8 cut(s) 529, 838, 1164, 1202, 1466, 1556, 1912, 1997
HpyF3I CTNAG 3 cut(s) 192, 849, 1708
HpySE526I ACGT 5 cut(s) 128, 156, 522, 1643, 1739
Hsp92I GRCGYC 3 cut(s) 14, 345, 708
HspAI GCGC 5 cut(s) 14, 253, 813, 1444, 1491
KasI GGCGCC 1 cut(s) 13
Kpn2I TCCGGA 1 cut(s) 663
KroI GCCGGC 1 cut(s) 961
KroNI GCCGGC 1 cut(s) 963
Ksp22I TGATCA 1 cut(s) 649
KspI CCGCGG 1 cut(s) 706
LguI GCTCTTC 1 cut(s) 1434
LmnI GCTCC 7 cut(s) 28, 36, 389, 404, 600, 1240, 2193
Lsp1109I GCAGC 4 cut(s) 327, 330, 333, 2104
LweI GCATC 5 cut(s) 489, 540, 1180, 1329, 1365
MaeI CTAG 3 cut(s) 1223, 1253, 2058
MaeII ACGT 5 cut(s) 128, 156, 522, 1643, 1739
MboII GAAGA 9 cut(s) 634, 688, 962, 1251, 1342, 1451, 1684, 1719, 1807
MfeI CAATTG 1 cut(s) 2139
MflI RGATCY 1 cut(s) 1724
MhlI GDGCHC 2 cut(s) 33, 1237
MlsI TGGCCA 1 cut(s) 1793
MluCI AATT 9 cut(s) 38, 653, 989, 1052, 1845, 1953, 2122, 2139, 2163
MluNI TGGCCA 1 cut(s) 1793
Mly113I GGCGCC 1 cut(s) 14
MlyI GAGTC 4 cut(s) 562, 598, 797, 1415
Mox20I TGGCCA 1 cut(s) 1793
Mph1103I ATGCAT 1 cut(s) 1558
MreI CGCCGGCG 1 cut(s) 961
MroI TCCGGA 1 cut(s) 663
MroNI GCCGGC 1 cut(s) 961
MscI TGGCCA 1 cut(s) 1793
MseI TTAA 2 cut(s) 473, 2015
MslI CAYNNNNRTG 4 cut(s) 144, 824, 1532, 1784
Msp20I TGGCCA 1 cut(s) 1793
MspA1I CMGCKG 1 cut(s) 705
MspR9I CCNGG 3 cut(s) 601, 796, 1068
MunI CAATTG 1 cut(s) 2139
MvaI CCWGG 1 cut(s) 1068
MvnI CGCG 3 cut(s) 60, 674, 705
NaeI GCCGGC 1 cut(s) 963
NarI GGCGCC 1 cut(s) 14
NciI CCSGG 2 cut(s) 601, 796
NcoI CCATGG 1 cut(s) 1428
NdeI CATATG 1 cut(s) 1897
NgoMIV GCCGGC 1 cut(s) 961
NlaIV GGNNCC 8 cut(s) 15, 400, 596, 1075, 1159, 1412, 1868, 1989
NmeAIII GCCGAG 2 cut(s) 103, 610
NmuCI GTSAC 7 cut(s) 466, 1111, 1321, 1378, 1756, 1945, 2065
NsiI ATGCAT 1 cut(s) 1558
OliI CACNNNNGTG 1 cut(s) 1784
PaeR7I CTCGAG 2 cut(s) 493, 1330
PagI TCATGA 1 cut(s) 1533
PciSI GCTCTTC 1 cut(s) 1434
PcsI WCGNNNNNNNCGW 1 cut(s) 1736
PdiI GCCGGC 1 cut(s) 963
PfeI GAWTC 2 cut(s) 484, 967
PflFI GACNNNGTC 1 cut(s) 607
PflMI CCANNNNNTGG 2 cut(s) 376, 1768
PinAI ACCGGT 1 cut(s) 915
PkrI GCNGC 7 cut(s) 317, 320, 323, 673, 707, 1636, 2094
PleI GAGTC 4 cut(s) 562, 598, 797, 1415
PluTI GGCGCC 1 cut(s) 17
PpsI GAGTC 4 cut(s) 562, 598, 797, 1415
PpuMI RGGWCCY 1 cut(s) 1987
PshAI GACNNNNGTC 1 cut(s) 1763
Psp124BI GAGCTC 1 cut(s) 33
Psp1406I AACGTT 1 cut(s) 1739
Psp5II RGGWCCY 1 cut(s) 1987
Psp6I CCWGG 1 cut(s) 1066
PspGI CCWGG 1 cut(s) 1066
PspN4I GGNNCC 8 cut(s) 15, 400, 596, 1075, 1159, 1412, 1868, 1989
PspPI GGNCC 8 cut(s) 247, 640, 697, 913, 1023, 1039, 1650, 1987
PspPPI RGGWCCY 1 cut(s) 1987
PspXI VCTCGAGB 1 cut(s) 493
PstI CTGCAG 1 cut(s) 1166
PsuI RGATCY 1 cut(s) 1724
PsyI GACNNNGTC 1 cut(s) 607
RsaI GTAC 4 cut(s) 449, 879, 950, 1374
RsaNI GTAC 4 cut(s) 448, 878, 949, 1373
RseI CAYNNNNRTG 4 cut(s) 144, 824, 1532, 1784
SacI GAGCTC 1 cut(s) 33
SacII CCGCGG 1 cut(s) 706
SalI GTCGAC 2 cut(s) 196, 409
SapI GCTCTTC 1 cut(s) 1434
SaqAI TTAA 2 cut(s) 473, 2015
SatI GCNGC 7 cut(s) 316, 319, 322, 672, 706, 1635, 2093
Sau96I GGNCC 8 cut(s) 247, 640, 697, 913, 1023, 1039, 1650, 1987
SchI GAGTC 4 cut(s) 562, 598, 797, 1415
ScrFI CCNGG 3 cut(s) 601, 796, 1068
SduI GDGCHC 2 cut(s) 33, 1237
SfaNI GCATC 5 cut(s) 489, 540, 1180, 1329, 1365
SfcI CTRYAG 1 cut(s) 1162
SfoI GGCGCC 1 cut(s) 15
Sfr274I CTCGAG 2 cut(s) 493, 1330
Sfr303I CCGCGG 1 cut(s) 706
SgrAI CRCCGGYG 1 cut(s) 961
SgrBI CCGCGG 1 cut(s) 706
SinI GGWCC 5 cut(s) 247, 913, 1023, 1039, 1987
SlaI CTCGAG 2 cut(s) 493, 1330
SmiMI CAYNNNNRTG 4 cut(s) 144, 824, 1532, 1784
SmlI CTYRAG 2 cut(s) 493, 1330
SmoI CTYRAG 2 cut(s) 493, 1330
Sse9I AATT 9 cut(s) 38, 653, 989, 1052, 1845, 1953, 2122, 2139, 2163
SsiI CCGC 9 cut(s) 58, 380, 582, 672, 703, 705, 717, 1370, 1635
SspDI GGCGCC 1 cut(s) 13
SspI AATATT 1 cut(s) 1392
SspMI CTAG 3 cut(s) 1223, 1253, 2058
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 3 cut(s) 599, 794, 1066
StyI CCWWGG 3 cut(s) 327, 1428, 1447
TaaI ACNGT 9 cut(s) 102, 466, 559, 682, 946, 953, 1157, 1562, 1783
TaiI ACGT 5 cut(s) 131, 159, 525, 1646, 1742
TaqII GACCGA 3 cut(s) 1040, 1309, 1554
TasI AATT 9 cut(s) 38, 653, 989, 1052, 1845, 1953, 2122, 2139, 2163
TatI WGTACW 2 cut(s) 447, 948
TauI GCSGC 3 cut(s) 674, 708, 1637
TfiI GAWTC 2 cut(s) 484, 967
Tru1I TTAA 2 cut(s) 473, 2015
Tru9I TTAA 2 cut(s) 473, 2015
TscAI CASTG 6 cut(s) 95, 951, 958, 1246, 1825, 2077
TseFI GTSAC 7 cut(s) 466, 1111, 1321, 1378, 1756, 1945, 2065
TseI GCWGC 4 cut(s) 315, 318, 321, 2092
Tsp45I GTSAC 7 cut(s) 466, 1111, 1321, 1378, 1756, 1945, 2065
TspDTI ATGAA 6 cut(s) 808, 1522, 1664, 1819, 1914, 1949
TspGWI ACGGA 3 cut(s) 865, 1028, 1351
TspRI CASTG 6 cut(s) 95, 951, 958, 1246, 1825, 2077
Tth111I GACNNNGTC 1 cut(s) 607
Van91I CCANNNNNTGG 2 cut(s) 376, 1768
VpaK11BI GGWCC 5 cut(s) 247, 913, 1023, 1039, 1987
XapI RAATTY 5 cut(s) 38, 989, 1845, 1953, 2163
XcmI CCANNNNNNNNNTGG 1 cut(s) 1084
XhoI CTCGAG 2 cut(s) 493, 1330
XmiI GTMKAC 2 cut(s) 197, 410
XspI CTAG 3 cut(s) 1223, 1253, 2058
Zsp2I ATGCAT 1 cut(s) 1558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.