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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 5 |
| AcuI | CTGAAG | 1 cut(s) | 204 |
| AgsI | TTSAA | 1 cut(s) | 28 |
| ApoI | RAATTY | 1 cut(s) | 5 |
| AspS9I | GGNCC | 1 cut(s) | 200 |
| AsuHPI | GGTGA | 1 cut(s) | 208 |
| AvaII | GGWCC | 1 cut(s) | 200 |
| BbsI | GAAGAC | 1 cut(s) | 140 |
| BccI | CCATC | 1 cut(s) | 213 |
| BfaI | CTAG | 1 cut(s) | 86 |
| Bme18I | GGWCC | 1 cut(s) | 200 |
| BmgT120I | GGNCC | 1 cut(s) | 200 |
| BmiI | GGNNCC | 1 cut(s) | 202 |
| BmsI | GCATC | 1 cut(s) | 40 |
| BpiI | GAAGAC | 1 cut(s) | 140 |
| BseGI | GGATG | 1 cut(s) | 177 |
| BseRI | GAGGAG | 1 cut(s) | 4 |
| BslFI | GGGAC | 2 cut(s) | 110, 186 |
| BsmFI | GGGAC | 2 cut(s) | 110, 186 |
| BspLI | GGNNCC | 1 cut(s) | 202 |
| Bst6I | CTCTTC | 1 cut(s) | 180 |
| BstF5I | GGATG | 1 cut(s) | 177 |
| BstV2I | GAAGAC | 1 cut(s) | 140 |
| BtsCI | GGATG | 1 cut(s) | 177 |
| Cfr13I | GGNCC | 1 cut(s) | 200 |
| Eam1104I | CTCTTC | 1 cut(s) | 180 |
| EarI | CTCTTC | 1 cut(s) | 180 |
| Eco47I | GGWCC | 1 cut(s) | 200 |
| Eco57I | CTGAAG | 1 cut(s) | 204 |
| FaiI | YATR | 2 cut(s) | 21, 213 |
| FaqI | GGGAC | 2 cut(s) | 110, 186 |
| FokI | GGATG | 1 cut(s) | 184 |
| FspBI | CTAG | 1 cut(s) | 86 |
| HphI | GGTGA | 1 cut(s) | 208 |
| Hpy188I | TCNGA | 1 cut(s) | 184 |
| HpyAV | CCTTC | 1 cut(s) | 26 |
| HpyCH4V | TGCA | 2 cut(s) | 47, 104 |
| LmnI | GCTCC | 1 cut(s) | 17 |
| LweI | GCATC | 1 cut(s) | 40 |
| MaeI | CTAG | 1 cut(s) | 86 |
| MboII | GAAGA | 3 cut(s) | 66, 140, 197 |
| MluCI | AATT | 3 cut(s) | 5, 61, 154 |
| MmeI | TCCRAC | 1 cut(s) | 149 |
| MnlI | CCTC | 3 cut(s) | 25, 181, 229 |
| NlaIV | GGNNCC | 1 cut(s) | 202 |
| PspN4I | GGNNCC | 1 cut(s) | 202 |
| PspPI | GGNCC | 1 cut(s) | 200 |
| Sau96I | GGNCC | 1 cut(s) | 200 |
| SetI | ASST | 4 cut(s) | 37, 112, 192, 221 |
| SfaNI | GCATC | 1 cut(s) | 40 |
| SgeI | CNNG | 5 cut(s) | 53, 68, 98, 138, 154 |
| SinI | GGWCC | 1 cut(s) | 200 |
| Sse9I | AATT | 3 cut(s) | 5, 61, 154 |
| SspMI | CTAG | 1 cut(s) | 86 |
| TasI | AATT | 3 cut(s) | 5, 61, 154 |
| TspGWI | ACGGA | 1 cut(s) | 149 |
| VpaK11BI | GGWCC | 1 cut(s) | 200 |
| XapI | RAATTY | 1 cut(s) | 5 |
| XspI | CTAG | 1 cut(s) | 86 |