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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 101 |
| AluBI | AGCT | 1 cut(s) | 129 |
| AluI | AGCT | 1 cut(s) | 129 |
| AoxI | GGCC | 1 cut(s) | 166 |
| ApoI | RAATTY | 1 cut(s) | 101 |
| BclI | TGATCA | 1 cut(s) | 144 |
| BfaI | CTAG | 1 cut(s) | 83 |
| BmsI | GCATC | 1 cut(s) | 51 |
| BseGI | GGATG | 1 cut(s) | 66 |
| BseRI | GAGGAG | 1 cut(s) | 123 |
| BseYI | CCCAGC | 1 cut(s) | 129 |
| BshFI | GGCC | 1 cut(s) | 168 |
| BsnI | GGCC | 1 cut(s) | 168 |
| Bsp143I | GATC | 1 cut(s) | 144 |
| BspANI | GGCC | 1 cut(s) | 168 |
| BssMI | GATC | 1 cut(s) | 144 |
| BstF5I | GGATG | 1 cut(s) | 66 |
| BstKTI | GATC | 1 cut(s) | 147 |
| BstMBI | GATC | 1 cut(s) | 144 |
| BsuRI | GGCC | 1 cut(s) | 168 |
| BtsCI | GGATG | 1 cut(s) | 66 |
| CviJI | RGCY | 2 cut(s) | 129, 168 |
| CviKI_1 | RGCY | 2 cut(s) | 129, 168 |
| DpnI | GATC | 1 cut(s) | 146 |
| DpnII | GATC | 1 cut(s) | 144 |
| FaiI | YATR | 4 cut(s) | 30, 44, 89, 99 |
| FbaI | TGATCA | 1 cut(s) | 144 |
| FokI | GGATG | 1 cut(s) | 73 |
| FspBI | CTAG | 1 cut(s) | 83 |
| GsaI | CCCAGC | 1 cut(s) | 133 |
| HaeIII | GGCC | 1 cut(s) | 168 |
| HinfI | GANTC | 1 cut(s) | 135 |
| Hpy166II | GTNNAC | 1 cut(s) | 139 |
| Hpy188I | TCNGA | 1 cut(s) | 107 |
| Hpy8I | GTNNAC | 1 cut(s) | 139 |
| HpyAV | CCTTC | 2 cut(s) | 152, 158 |
| HpyCH4V | TGCA | 1 cut(s) | 25 |
| Ksp22I | TGATCA | 1 cut(s) | 144 |
| Kzo9I | GATC | 1 cut(s) | 144 |
| LpnPI | CCDG | 4 cut(s) | 84, 106, 115, 155 |
| LweI | GCATC | 1 cut(s) | 51 |
| MaeI | CTAG | 1 cut(s) | 83 |
| MalI | GATC | 1 cut(s) | 146 |
| MboI | GATC | 1 cut(s) | 144 |
| MluCI | AATT | 1 cut(s) | 101 |
| MlyI | GAGTC | 1 cut(s) | 144 |
| MnlI | CCTC | 1 cut(s) | 101 |
| NdeII | GATC | 1 cut(s) | 144 |
| PleI | GAGTC | 1 cut(s) | 143 |
| PpsI | GAGTC | 1 cut(s) | 143 |
| PspFI | CCCAGC | 1 cut(s) | 129 |
| Sau3AI | GATC | 1 cut(s) | 144 |
| SchI | GAGTC | 1 cut(s) | 144 |
| SetI | ASST | 2 cut(s) | 131, 144 |
| SfaNI | GCATC | 1 cut(s) | 51 |
| SgeI | CNNG | 5 cut(s) | 83, 95, 133, 142, 154 |
| Sse9I | AATT | 1 cut(s) | 101 |
| SspMI | CTAG | 1 cut(s) | 83 |
| TasI | AATT | 1 cut(s) | 101 |
| XapI | RAATTY | 1 cut(s) | 101 |
| XspI | CTAG | 1 cut(s) | 83 |