MD10G1006400.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000105
Physical Location & Seq
Reverse (-)
954412 .. 959702
5291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1006400.v1.1.491

Sequence Viewer

Length: 3921 bp
ATGGTGCAAACAAATAAGTACGATGTCTACCTCAGTTTCAGAGGCGAAGACACCCGCATGAATTTCGCAAGCCATCTAGAGGCGGCTCTGAGTAGAAATGGAGTCGTCGTATGGAGAGACGAAGATTGTCTACTTCCTGGGCCAGAACCCATTAACTCACAAATCCGCAAGGCAATCGAAAGTTCAAGAATTTCAATTGTCATTCTTTCAAGAAATTACGCCAGTTCAAAATGGTGCTTGGATGAGCTTCAACAAATTCTCGATTGTAGGCAAGTGACTGGCCAGATAGTTTTTCCTGTCTTCTATGATGTGGACCCATCCGACGTACGACATCAGACAGGCCGTTTCGGTGATGCTTTTGCCGAGCACGAACAACGTTTCAAGGACAATATCCACAAAGTGCGCGCCTGGAGGGATGCTCTTCGTCAATTAGCCGATCTGTCTGGATATGCGCTACTCGAGGATCGGTATGAGTCACAATCAGTCGCACGAATTGTTGAATTCATATTGAATACATTGCAACCTGTGGCACCTGATGTTGAGCAGCAGAGATTTCCGTTGGGATCCATGGCCACCGCGCAACCAACTGATCACGATGTCTACCTCAGTTTCAGAGGCGAAGACACTCGCATGATTTTCGTAAGCCATCTAGAGGCGGCTCTGAGTAGAAACGGAGTCGTCGTATGGAGAGACGAAGATTGTCTACGTCCTGGGGCAGAACCCATTAACTCACAAATCCGCAAGGCAATCGAAAGTTCAAGAATTTCAATTGTCATTCTTTCAAGAAATTACGCCAGTTCAACATGGTGCTTGGATGAGCTTCAACAAATTCTCGATTGTAGGCAAGTGACTGGCCAGAAAGTTTTACCTGTCTTCTATGATGTGGACCCATCCGATGTACGACATCAGACAGGCGGTTTCGGTGATGCTTTTGCCAAGCACGAACAACGTTTCAAGGACAATATCGAAAAAGTGCACGCCTGGAGGGACGCTCTTCGTCAAGTAGCCGATCTGTCTGGATTTGTGCTACGCGAGGATCGGTATGAGTCACAATCAGTCGAACGAATTGTTGTATTCATATTGAATGCATTGCAACCCGTGGCATCTGAAGTTGAGCAGCTAGTGGGAATTGGATCAAGAGTAGAGGAACTATATAGGCTTTTGGATATCGAATCAAATGATGTTCGCTTCATAGGGATATGGGGGATGGGTGGGATAGGCAAGACAACTATCGCCGATGTGATTTTTAACAACATTTCCATTGAATTTGAAATTTTCACCCGTATTCGTAATATTAGGGAGCTAAGTGAAACACAGGGTGATCTGCAACTGCAAAAGAAACTTCTTTATGAGGCTTTGAGGCAAGACATAGATGTTCGAAATGTTAACGAGGGAGCCACAATGATAAAGAACATTCTACGTAAGAGAAAGGTTCTTCTCATTTTAGACGATGTTGACAACTTACACCAACTGGAATCATTGGCTGGAAACAGGGAATGGTTTGGTTTGGGGAGCAGAGTCATCGTTACGACTAGAAATGAGAAATTGCTAAGAAAGCATGGTGTAGATAGCATATTTGAAGTTAAGGAATTAACTGAGGGTGAATCGCTTCAGCTCTTCAATATTTACGCCTTTAAAAGTGTTGAACCCCCAGAAGATTATTTGAATCTGTCTAAACAGTTTGTTAATTATTGTAGAGGCATTCCCCTTGCAATCAAGGTTTTGGGATCCTCACTGTTCCACAGAAGTCTTGAAGAGTGGCATAGCGTATTGAAAAAACGGGAGCACTTTCCTGATGAAACAGTTTCTGAAATACTTGAAATAAGTTATCATGGACTGGCGGACAATGAGAAAAGAATGTTTCTAGATATTGCTTGTTTCTTTCAAGGAATGGACAAGGAACGAGTGGTAGAGATACTAGATTGTTTTGGATTTAATCCCAAGGAGGGGATGGAAGCTCTCATTGTGAGATCTCTCATAACTATTTCAAATAACAAAGTGTTGATGAATGGGTTGATACAAGAAATGGGACAACAAGTGGTACGCAGAGAAGCTGTTAATGATCCTGGAAAGCAAAGCAGGTTGTGGCTTCATGAGGACGTCATTCATGTATTGAAGAATAATATGGGAACAAATGCAGTTGAAGGTATAGCCTTAGACTTGCCTAAATTGGAAGAGGCATGTTGGGACTTGGAGGCCTTCTCGATGATGCATAATCTCAGATTTCTCCAAATTCATAATCTGCGAACGACCCAAGGCCCAAAAAAACTTTCTAATGCCTTAAAGTTTCTTGAATGGAGTGGGTATCCTTCAAAATTTCTCCCACAAGAATTTGAACTTGAGCAACTTTGTGAAGTTAACTTGTGCCATAGCAGCATTGAACAACTTTGGAGTAGAACCAAGTTATTAGGCAATCTGAAATTCGTCAACGTTAGCTACTCCCAGAACCTGACCAGGACCCCGGACTTTACAGTTACTCCAAATCTTCATAGATTAATTCTTGAAGGCTGCATAAACTTGGTGATGATCCACCCATCCATAGCTGAGCTCAAAAGGCTTATTTTTTTAAATCTTAAGGACTGCAGAAGTCTTGAACACCTTCCCAAACGTTTTGGAACAGAAAGTCTTCAGATTCTTATTCTTTCCGGTTGTTCAAAAATTAGTAAAGTTCCAGAATTTGTGAGACCTATGGAAAACTTGTTGGAACTTTATTTAGATAAGACTGCTGTAGAAGAATTACCTTCTTCAATCGAATGTCTGACTGGCCTTACTTTATTAAACCTGAGAGATTGCAGAAATCTCAGTTCCCTTGCAAGCAGTACTTGCCAGTTGCGCTCCCTCAAAAGTCTCAATCTCAGTGGATGCTCAAAGCTTGAAGAACTGCCAAAAAACTTGGGAAAGATGGTTTGTTTGAAGGAGCTTGATGTGGGCGGTACCTCTATTAATGATCTACCATCCTCCATTTGCCGTCTGGAAAATCTTGAATTGTTATCCCTTTGTGGTTGCAAATCGATGCGAAGAAAGAAGACAAGTCCAATTAGTTTTCTGTTGCCGACCACTGATTCTAGTTTGCTTTCTTTAACAGTACTGAATATCAGTGACTGTAATCTTGAGGAGGTAACAATCCTGGAAAGTCTCGGCTCCTTGTCCTCAATAGTATCGCTTAATCTAAGTAAAAACAGTTTTGTTAGTCTTCCCAAGAGCATCATAGAGCTGTCTAAGCTTCAGATTTTGAATTTGGGTGGTTGTAAGAGTCTTAAAAAATTGCCTGACCTTTCAACAGAATTGAACTTCAGTGTTGAGGGAAAAGGCAGTTATTCACAGGAAAGGCTGTCATCATGTTTCAGTTTTATTAATTGCTTTGAAGTGGTTGACGACCAAGGCTGCAACAATGTAGCATTTGCAGCATTAAGGAGATTCCTTGAGGGAATCCCTTACGAAGGGAATAAATTTGAAGCTATATATCCTGGCAGTGAAATTTCTGAGTGGTTTAGTTATCGAAGTGAGAGGCCTGTGGTAAGCATGGAGCTCCCTCAACGCTGGTATAAGAACAAGTGGATGGGATATGTATTGTGTGCTGTTTTCACTCTCCGTCAACGACTCCCACCTAATCTGCTTGGTAAATGGAACTACGGAACCCATAGCACTGCACATGGTCTTCATTGCGAAGTGAAGCCTGGGAATTTGGGTGTAGGTGGCTGGTGTCCTTCCTTCGCCTGCAGCGAAGAGTTAGGGCAAATTGAGAAAGAGCACCTTTGGCTATCCTTTGTATCTGGTGAATACTTTGGTACAACATGGCAAGACAGTTGTCATCACCTTGAGTTTACATTTAAAACCTTAGGCCCTGGTTTGGAGGTGAAAAGGTGTGGAGTCCGTCTCATCTACGAGAGAGATTTGGTTGGATTAAAGCAACCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1307

Amino Acids

148.55

Weight (kDa)

5.91

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 6 - 179 5e-48 TIR domain
TIR_2 PF13676 9 - 107 1.6e-16 TIR domain
TIR PF01582 198 - 370 2.5e-48 TIR domain
TIR_2 PF13676 200 - 298 5.6e-17 TIR domain
NB-ARC PF00931 381 - 546 2.7e-23 NB-ARC domain
WHD_ROQ1 PF23282 615 - 683 3.3e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 875 - 960 6.3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 919 - 1003 2.1e-10 Leucine-rich repeat region
LRR_14 PF23598 1022 - 1094 6.5e-07 Leucine-rich repeat region
C-JID PF20160 1156 - 1298 6.6e-15 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2103
Acc36I ACCTGC 1 cut(s) 2070
Acc65I GGTACC 1 cut(s) 2933
AccB1I GGYRCC 2 cut(s) 529, 2933
AccI GTMKAC 4 cut(s) 27, 130, 600, 703
AccII CGCG 3 cut(s) 405, 578, 1032
AciI CCGC 9 cut(s) 55, 83, 166, 576, 656, 739, 915, 1841, 2931
AclI AACGTT 4 cut(s) 376, 949, 2430, 2608
AclWI GGATC 9 cut(s) 471, 558, 571, 1044, 1141, 1722, 1735, 2057, 2521
AcoI YGGCCR 3 cut(s) 280, 570, 853
AcuI CTGAAG 5 cut(s) 1128, 1595, 2612, 3209, 3277
AcyI GRCGYC 1 cut(s) 2100
AdeI CACNNNGTG 2 cut(s) 400, 1319
AfaI GTAC 8 cut(s) 20, 327, 900, 2043, 2821, 2935, 3087, 3790
AfiI CCNNNNNNNGG 6 cut(s) 79, 652, 1946, 1947, 3440, 3850
AflII CTTAAG 1 cut(s) 2573
AjuI GAANNNNNNNTTGG 2 cut(s) 2254, 2286
Alw21I GWGCWC 6 cut(s) 369, 978, 1788, 2550, 3531, 3753
Alw26I GTCTC 6 cut(s) 111, 684, 2677, 2852, 3140, 3881
Alw44I GTGCAC 1 cut(s) 974
AlwI GGATC 9 cut(s) 471, 558, 571, 1044, 1141, 1722, 1735, 2057, 2521
AlwNI CAGNNNCTG 3 cut(s) 1808, 2449, 3102
Ama87I CYCGRG 1 cut(s) 458
ApaLI GTGCAC 1 cut(s) 974
ApeKI GCWGC 7 cut(s) 544, 1117, 2373, 2508, 3384, 3404, 3720
AseI ATTAAT 3 cut(s) 2495, 2943, 3354
Asp700I GAANNNNTTC 3 cut(s) 1608, 2598, 2625
Asp718I GGTACC 1 cut(s) 2933
AspLEI GCGC 5 cut(s) 405, 407, 454, 580, 2835
AspS9I GGNCC 6 cut(s) 140, 313, 886, 2258, 2457, 3842
AsuC2I CCSGG 1 cut(s) 2462
AsuHPI GGTGA 9 cut(s) 362, 935, 1270, 1331, 1613, 2533, 3788, 3806, 3868
AsuII TTCGAA 1 cut(s) 1378
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 3 cut(s) 313, 886, 2457
AxyI CCTNAGG 1 cut(s) 3838
BaeGI GKGCMC 1 cut(s) 978
BalI TGGCCA 3 cut(s) 282, 572, 855
BamHI GGATCC 2 cut(s) 563, 1727
BanI GGYRCC 2 cut(s) 529, 2933
BanII GRGCYC 2 cut(s) 2550, 3531
BarI GAAGNNNNNNTAC 4 cut(s) 114, 146, 687, 719
BbsI GAAGAC 8 cut(s) 54, 292, 627, 865, 2618, 3032, 3185, 3650
Bbv12I GWGCWC 6 cut(s) 369, 978, 1788, 2550, 3531, 3753
BbvI GCAGC 7 cut(s) 556, 1129, 2385, 2495, 3371, 3416, 3732
BceAI ACGGC 2 cut(s) 327, 2952
BciVI GTATCC 1 cut(s) 2316
BclI TGATCA 1 cut(s) 589
BcnI CCSGG 1 cut(s) 2462
BcoDI GTCTC 6 cut(s) 111, 684, 2677, 2852, 3140, 3881
BfaI CTAG 7 cut(s) 77, 650, 1121, 1533, 1865, 1919, 3066
BfmI CTRYAG 3 cut(s) 2581, 2727, 3718
BfrI CTTAAG 1 cut(s) 2573
BfuAI ACCTGC 1 cut(s) 2070
BfuI GTATCC 1 cut(s) 2316
BglII AGATCT 1 cut(s) 1970
BisI GCNGC 9 cut(s) 84, 545, 657, 1118, 2374, 2509, 3385, 3405, 3721
BlpI GCTNAGC 1 cut(s) 2544
BlsI GCNGC 9 cut(s) 85, 546, 658, 1119, 2375, 2510, 3386, 3406, 3722
BmcAI AGTACT 2 cut(s) 2821, 3087
Bme18I GGWCC 3 cut(s) 313, 886, 2457
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 6 cut(s) 140, 313, 886, 2258, 2457, 3842
BmsI GCATC 8 cut(s) 343, 406, 916, 1112, 2199, 2852, 3003, 3213
BoxI GACNNNNGTC 1 cut(s) 3807
BpiI GAAGAC 8 cut(s) 54, 292, 627, 865, 2618, 3032, 3185, 3650
BplI GAGNNNNNCTC 8 cut(s) 403, 435, 976, 1008, 2186, 2218, 3861, 3893
BpmI CTGGAG 2 cut(s) 430, 1003
Bpu1102I GCTNAGC 1 cut(s) 2544
Bpu14I TTCGAA 1 cut(s) 1378
BpuEI CTTGAG 4 cut(s) 2360, 3131, 3443, 3839
BpuMI CCSGG 1 cut(s) 2462
Bsa29I ATCGAT 1 cut(s) 3011
BsaAI YACGTR 1 cut(s) 1421
BsaHI GRCGYC 1 cut(s) 2100
BsaI GGTCTC 1 cut(s) 2677
BsaWI WCCGGW 1 cut(s) 2645
BsaXI ACNNNNNCTCC 6 cut(s) 93, 123, 666, 696, 2461, 2491
Bsc4I CCNNNNNNNGG 6 cut(s) 79, 652, 1946, 1947, 3440, 3850
Bse1I ACTGG 8 cut(s) 222, 283, 795, 856, 1476, 1842, 2767, 2827
Bse21I CCTNAGG 1 cut(s) 3838
Bse3DI GCAATG 3 cut(s) 515, 1088, 3661
BseCI ATCGAT 1 cut(s) 3011
BseLI CCNNNNNNNGG 6 cut(s) 79, 652, 1946, 1947, 3440, 3850
BseMI GCAATG 3 cut(s) 515, 1088, 3661
BseNI ACTGG 8 cut(s) 222, 283, 795, 856, 1476, 1842, 2767, 2827
BsePI GCGCGC 1 cut(s) 403
BseRI GAGGAG 1 cut(s) 3128
BseSI GKGCMC 1 cut(s) 978
BseXI GCAGC 7 cut(s) 556, 1129, 2385, 2495, 3371, 3416, 3732
BsgI GTGCAG 1 cut(s) 3633
Bsh1236I CGCG 3 cut(s) 405, 578, 1032
BshNI GGYRCC 2 cut(s) 529, 2933
BshVI ATCGAT 1 cut(s) 3011
BsiHKAI GWGCWC 6 cut(s) 369, 978, 1788, 2550, 3531, 3753
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 2 cut(s) 2462, 2646
BsiWI CGTACG 1 cut(s) 325
BslFI GGGAC 3 cut(s) 1001, 2043, 2201
BslI CCNNNNNNNGG 6 cut(s) 79, 652, 1946, 1947, 3440, 3850
BsmAI GTCTC 6 cut(s) 111, 684, 2677, 2852, 3140, 3881
BsmBI CGTCTC 3 cut(s) 111, 684, 3881
BsmFI GGGAC 3 cut(s) 1001, 2043, 2201
BsmI GAATGC 2 cut(s) 1090, 1701
Bso31I GGTCTC 1 cut(s) 2677
BsoBI CYCGRG 1 cut(s) 458
Bsp119I TTCGAA 1 cut(s) 1378
Bsp1286I GDGCHC 6 cut(s) 369, 978, 1788, 2550, 3531, 3753
Bsp1720I GCTNAGC 1 cut(s) 2544
Bsp19I CCATGG 1 cut(s) 567
BspACI CCGC 9 cut(s) 55, 83, 166, 576, 656, 739, 915, 1841, 2931
BspDI ATCGAT 1 cut(s) 3011
BspFNI CGCG 3 cut(s) 405, 578, 1032
BspHI TCATGA 1 cut(s) 2092
BspMAI CTGCAG 2 cut(s) 2585, 3722
BspMI ACCTGC 1 cut(s) 2070
BspPI GGATC 9 cut(s) 471, 558, 571, 1044, 1141, 1722, 1735, 2057, 2521
BspQI GCTCTTC 3 cut(s) 426, 999, 1622
BspT104I TTCGAA 1 cut(s) 1378
BspT107I GGYRCC 2 cut(s) 529, 2933
BspTI CTTAAG 1 cut(s) 2573
BspTNI GGTCTC 1 cut(s) 2677
BsrDI GCAATG 3 cut(s) 515, 1088, 3661
BsrI ACTGG 8 cut(s) 222, 283, 795, 856, 1476, 1842, 2767, 2827
BssHII GCGCGC 1 cut(s) 403
BssNI GRCGYC 1 cut(s) 2100
BssT1I CCWWGG 4 cut(s) 567, 1941, 2254, 3379
Bst4CI ACNGT 8 cut(s) 1680, 1737, 1804, 2473, 3085, 3104, 3182, 3806
Bst6I CTCTTC 6 cut(s) 426, 999, 1622, 1749, 2169, 3720
BstACI GRCGYC 1 cut(s) 2100
BstAFI CTTAAG 1 cut(s) 2573
BstAPI GCANNNNNTGC 1 cut(s) 2823
BstBAI YACGTR 1 cut(s) 1421
BstBI TTCGAA 1 cut(s) 1378
BstC8I GCNNGC 5 cut(s) 70, 405, 978, 2815, 3718
BstDSI CCRYGG 2 cut(s) 567, 1098
BstENI CCTNNNNNAGG 1 cut(s) 3438
BstFNI CGCG 3 cut(s) 405, 578, 1032
BstHHI GCGC 5 cut(s) 405, 407, 454, 580, 2835
BstMAI GTCTC 6 cut(s) 111, 684, 2677, 2852, 3140, 3881
BstMWI GCNNNNNNNGC 8 cut(s) 1555, 2373, 2554, 2823, 2832, 3220, 3404, 3757
BstNSI RCATGY 1 cut(s) 2184
BstPAI GACNNNNGTC 1 cut(s) 3807
BstSFI CTRYAG 3 cut(s) 2581, 2727, 3718
BstSLI GKGCMC 1 cut(s) 978
BstSNI TACGTA 1 cut(s) 1421
BstUI CGCG 3 cut(s) 405, 578, 1032
BstV1I GCAGC 7 cut(s) 556, 1129, 2385, 2495, 3371, 3416, 3732
BstV2I GAAGAC 8 cut(s) 54, 292, 627, 865, 2618, 3032, 3185, 3650
BstX2I RGATCY 3 cut(s) 563, 1727, 1970
BstYI RGATCY 3 cut(s) 563, 1727, 1970
Bsu15I ATCGAT 1 cut(s) 3011
Bsu36I CCTNAGG 1 cut(s) 3838
BsuI GTATCC 1 cut(s) 2316
BsuTUI ATCGAT 1 cut(s) 3011
BtgI CCRYGG 2 cut(s) 567, 1098
BtsI GCAGTG 2 cut(s) 3478, 3645
BtsIMutI CAGTG 7 cut(s) 1733, 2863, 3057, 3103, 3301, 3478, 3645
BveI ACCTGC 1 cut(s) 2070
Cac8I GCNNGC 5 cut(s) 70, 405, 978, 2815, 3718
CaiI CAGNNNCTG 3 cut(s) 1808, 2449, 3102
CciI TCATGA 1 cut(s) 2092
CfoI GCGC 5 cut(s) 405, 407, 454, 580, 2835
Cfr13I GGNCC 6 cut(s) 140, 313, 886, 2258, 2457, 3842
ClaI ATCGAT 1 cut(s) 3011
CseI GACGC 1 cut(s) 998
Csp6I GTAC 8 cut(s) 19, 326, 899, 2042, 2820, 2934, 3086, 3789
CspCI CAANNNNNGTGG 2 cut(s) 2839, 2874
CviQI GTAC 8 cut(s) 19, 326, 899, 2042, 2820, 2934, 3086, 3789
DraI TTTAAA 3 cut(s) 1636, 2568, 3832
DraIII CACNNNGTG 2 cut(s) 400, 1319
EaeI YGGCCR 3 cut(s) 280, 570, 853
Eam1104I CTCTTC 6 cut(s) 426, 999, 1622, 1749, 2169, 3720
EarI CTCTTC 6 cut(s) 426, 999, 1622, 1749, 2169, 3720
EciI GGCGGA 1 cut(s) 1856
Ecl136II GAGCTC 2 cut(s) 2548, 3529
Eco105I TACGTA 1 cut(s) 1421
Eco130I CCWWGG 4 cut(s) 567, 1941, 2254, 3379
Eco147I AGGCCT 2 cut(s) 2198, 3511
Eco24I GRGCYC 2 cut(s) 2550, 3531
Eco31I GGTCTC 1 cut(s) 2677
Eco32I GATATC 1 cut(s) 1168
Eco47I GGWCC 3 cut(s) 313, 886, 2457
Eco53kI GAGCTC 2 cut(s) 2548, 3529
Eco57I CTGAAG 5 cut(s) 1128, 1595, 2612, 3209, 3277
Eco81I CCTNAGG 1 cut(s) 3838
Eco88I CYCGRG 1 cut(s) 458
EcoICRI GAGCTC 2 cut(s) 2548, 3529
EcoNI CCTNNNNNAGG 1 cut(s) 3438
EcoO109I RGGNCCY 2 cut(s) 2457, 3842
EcoRI GAATTC 1 cut(s) 500
EcoRV GATATC 1 cut(s) 1168
EcoT14I CCWWGG 4 cut(s) 567, 1941, 2254, 3379
EcoT22I ATGCAT 2 cut(s) 1090, 2214
EcoT38I GRGCYC 2 cut(s) 2550, 3531
ErhI CCWWGG 4 cut(s) 567, 1941, 2254, 3379
Esp3I CGTCTC 3 cut(s) 111, 684, 3881
FalI AAGNNNNNCTT 4 cut(s) 2806, 2838, 3738, 3770
FaqI GGGAC 3 cut(s) 1001, 2043, 2201
FauI CCCGC 1 cut(s) 62
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 4 cut(s) 27, 130, 600, 703
Fnu4HI GCNGC 9 cut(s) 84, 545, 657, 1118, 2374, 2509, 3385, 3405, 3721
FriOI GRGCYC 2 cut(s) 2550, 3531
Fsp4HI GCNGC 9 cut(s) 84, 545, 657, 1118, 2374, 2509, 3385, 3405, 3721
FspBI CTAG 7 cut(s) 77, 650, 1121, 1533, 1865, 1919, 3066
GlaI GCGC 5 cut(s) 404, 406, 453, 579, 2834
GluI GCNGC 9 cut(s) 84, 545, 657, 1118, 2374, 2509, 3385, 3405, 3721
GsuI CTGGAG 2 cut(s) 430, 1003
HapII CCGG 2 cut(s) 2462, 2646
HgaI GACGC 1 cut(s) 998
HhaI GCGC 5 cut(s) 405, 407, 454, 580, 2835
Hin1I GRCGYC 1 cut(s) 2100
Hin6I GCGC 5 cut(s) 403, 405, 452, 578, 2833
HinP1I GCGC 5 cut(s) 403, 405, 452, 578, 2833
HincII GTYRAC 6 cut(s) 1387, 1456, 2359, 2428, 3373, 3596
HindII GTYRAC 6 cut(s) 1387, 1456, 2359, 2428, 3373, 3596
HindIII AAGCTT 2 cut(s) 2870, 3221
HpaI GTTAAC 2 cut(s) 1387, 2359
HpaII CCGG 2 cut(s) 2462, 2646
HphI GGTGA 9 cut(s) 362, 935, 1270, 1331, 1613, 2533, 3788, 3806, 3868
Hpy99I CGWCG 3 cut(s) 110, 326, 683
HpyCH4III ACNGT 8 cut(s) 1680, 1737, 1804, 2473, 3085, 3104, 3182, 3806
HpyCH4IV ACGT 8 cut(s) 324, 376, 706, 949, 1420, 2100, 2430, 2608
HpyF10VI GCNNNNNNNGC 8 cut(s) 1555, 2373, 2554, 2823, 2832, 3220, 3404, 3757
HpySE526I ACGT 8 cut(s) 324, 376, 706, 949, 1420, 2100, 2430, 2608
Hsp92I GRCGYC 1 cut(s) 2100
HspAI GCGC 5 cut(s) 403, 405, 452, 578, 2833
KpnI GGTACC 1 cut(s) 2937
Ksp22I TGATCA 1 cut(s) 589
KspAI GTTAAC 2 cut(s) 1387, 2359
LguI GCTCTTC 3 cut(s) 426, 999, 1622
LmnI GCTCC 9 cut(s) 1300, 1394, 1512, 1783, 2840, 2917, 3146, 3526, 3534
Lsp1109I GCAGC 7 cut(s) 556, 1129, 2385, 2495, 3371, 3416, 3732
LweI GCATC 8 cut(s) 343, 406, 916, 1112, 2199, 2852, 3003, 3213
MaeI CTAG 7 cut(s) 77, 650, 1121, 1533, 1865, 1919, 3066
MaeII ACGT 8 cut(s) 324, 376, 706, 949, 1420, 2100, 2430, 2608
MaeIII GTNAC 8 cut(s) 274, 474, 847, 1047, 1525, 2473, 3098, 3118
MfeI CAATTG 2 cut(s) 195, 768
MflI RGATCY 3 cut(s) 563, 1727, 1970
MhlI GDGCHC 6 cut(s) 369, 978, 1788, 2550, 3531, 3753
MlsI TGGCCA 3 cut(s) 282, 572, 855
MluNI TGGCCA 3 cut(s) 282, 572, 855
MlyI GAGTC 8 cut(s) 111, 482, 684, 1055, 1527, 3262, 3594, 3879
MmeI TCCRAC 3 cut(s) 345, 2682, 3880
Mox20I TGGCCA 3 cut(s) 282, 572, 855
Mph1103I ATGCAT 2 cut(s) 1090, 2214
MroXI GAANNNNTTC 3 cut(s) 1608, 2598, 2625
MscI TGGCCA 3 cut(s) 282, 572, 855
MslI CAYNNNNRTG 2 cut(s) 56, 629
Msp20I TGGCCA 3 cut(s) 282, 572, 855
MspCI CTTAAG 1 cut(s) 2573
MspI CCGG 2 cut(s) 2462, 2646
MunI CAATTG 2 cut(s) 195, 768
Mva1269I GAATGC 2 cut(s) 1090, 1701
MvnI CGCG 3 cut(s) 405, 578, 1032
MwoI GCNNNNNNNGC 8 cut(s) 1555, 2373, 2554, 2823, 2832, 3220, 3404, 3757
NciI CCSGG 1 cut(s) 2462
NcoI CCATGG 1 cut(s) 567
NmeAIII GCCGAG 2 cut(s) 388, 3117
NmuCI GTSAC 5 cut(s) 274, 474, 847, 1047, 3098
NsiI ATGCAT 2 cut(s) 1090, 2214
NspI RCATGY 1 cut(s) 2184
NspV TTCGAA 1 cut(s) 1378
PaeR7I CTCGAG 1 cut(s) 458
PagI TCATGA 1 cut(s) 2092
PauI GCGCGC 1 cut(s) 403
PceI AGGCCT 2 cut(s) 2198, 3511
PciSI GCTCTTC 3 cut(s) 426, 999, 1622
PcsI WCGNNNNNNNCGW 2 cut(s) 678, 3720
PctI GAATGC 2 cut(s) 1090, 1701
PdmI GAANNNNTTC 3 cut(s) 1608, 2598, 2625
PfeI GAWTC 8 cut(s) 1172, 1475, 1604, 1666, 2632, 3062, 3417, 3429
Pfl23II CGTACG 1 cut(s) 325
PfoI TCCNGGA 2 cut(s) 2065, 3126
PkrI GCNGC 9 cut(s) 85, 546, 658, 1119, 2375, 2510, 3386, 3406, 3722
PleI GAGTC 8 cut(s) 110, 481, 683, 1054, 1526, 3261, 3594, 3878
PpsI GAGTC 8 cut(s) 110, 481, 683, 1054, 1526, 3261, 3594, 3878
Ppu21I YACGTR 1 cut(s) 1421
PpuMI RGGWCCY 1 cut(s) 2457
PshAI GACNNNNGTC 1 cut(s) 3807
PshBI ATTAAT 3 cut(s) 2495, 2943, 3354
Psp124BI GAGCTC 2 cut(s) 2550, 3531
Psp1406I AACGTT 4 cut(s) 376, 949, 2430, 2608
Psp5II RGGWCCY 1 cut(s) 2457
PspLI CGTACG 1 cut(s) 325
PspPI GGNCC 6 cut(s) 140, 313, 886, 2258, 2457, 3842
PspPPI RGGWCCY 1 cut(s) 2457
PspXI VCTCGAGB 1 cut(s) 458
PstI CTGCAG 2 cut(s) 2585, 3722
PstNI CAGNNNCTG 3 cut(s) 1808, 2449, 3102
PsuI RGATCY 3 cut(s) 563, 1727, 1970
PteI GCGCGC 1 cut(s) 403
RsaI GTAC 8 cut(s) 20, 327, 900, 2043, 2821, 2935, 3087, 3790
RsaNI GTAC 8 cut(s) 19, 326, 899, 2042, 2820, 2934, 3086, 3789
RseI CAYNNNNRTG 2 cut(s) 56, 629
SacI GAGCTC 2 cut(s) 2550, 3531
SapI GCTCTTC 3 cut(s) 426, 999, 1622
SatI GCNGC 9 cut(s) 84, 545, 657, 1118, 2374, 2509, 3385, 3405, 3721
Sau96I GGNCC 6 cut(s) 140, 313, 886, 2258, 2457, 3842
ScaI AGTACT 2 cut(s) 2821, 3087
SchI GAGTC 8 cut(s) 111, 482, 684, 1055, 1527, 3262, 3594, 3879
SduI GDGCHC 6 cut(s) 369, 978, 1788, 2550, 3531, 3753
SfaNI GCATC 8 cut(s) 343, 406, 916, 1112, 2199, 2852, 3003, 3213
SfcI CTRYAG 3 cut(s) 2581, 2727, 3718
Sfr274I CTCGAG 1 cut(s) 458
SfuI TTCGAA 1 cut(s) 1378
SinI GGWCC 3 cut(s) 313, 886, 2457
SlaI CTCGAG 1 cut(s) 458
SmiMI CAYNNNNRTG 2 cut(s) 56, 629
SmlI CTYRAG 6 cut(s) 458, 2339, 2573, 3110, 3422, 3818
SmoI CTYRAG 6 cut(s) 458, 2339, 2573, 3110, 3422, 3818
SnaBI TACGTA 1 cut(s) 1421
SseBI AGGCCT 2 cut(s) 2198, 3511
SsiI CCGC 9 cut(s) 55, 83, 166, 576, 656, 739, 915, 1841, 2931
SspI AATATT 2 cut(s) 1294, 1624
SspMI CTAG 7 cut(s) 77, 650, 1121, 1533, 1865, 1919, 3066
SstI GAGCTC 2 cut(s) 2550, 3531
StuI AGGCCT 2 cut(s) 2198, 3511
StyI CCWWGG 4 cut(s) 567, 1941, 2254, 3379
TaaI ACNGT 8 cut(s) 1680, 1737, 1804, 2473, 3085, 3104, 3182, 3806
TaiI ACGT 8 cut(s) 327, 379, 709, 952, 1423, 2103, 2433, 2611
TatI WGTACW 2 cut(s) 2819, 3085
TauI GCSGC 2 cut(s) 86, 659
TfiI GAWTC 8 cut(s) 1172, 1475, 1604, 1666, 2632, 3062, 3417, 3429
TscAI CASTG 7 cut(s) 1740, 2863, 3064, 3103, 3301, 3478, 3652
TseFI GTSAC 5 cut(s) 274, 474, 847, 1047, 3098
TseI GCWGC 7 cut(s) 544, 1117, 2373, 2508, 3384, 3404, 3720
Tsp45I GTSAC 5 cut(s) 274, 474, 847, 1047, 3098
TspGWI ACGGA 5 cut(s) 546, 687, 3581, 3648, 3863
TspRI CASTG 7 cut(s) 1740, 2863, 3064, 3103, 3301, 3478, 3652
Vha464I CTTAAG 1 cut(s) 2573
VneI GTGCAC 1 cut(s) 974
VpaK11BI GGWCC 3 cut(s) 313, 886, 2457
VspI ATTAAT 3 cut(s) 2495, 2943, 3354
XagI CCTNNNNNAGG 1 cut(s) 3438
XbaI TCTAGA 3 cut(s) 76, 649, 1864
XceI RCATGY 1 cut(s) 2184
XcmI CCANNNNNNNNNTGG 2 cut(s) 1948, 2968
XhoI CTCGAG 1 cut(s) 458
XmiI GTMKAC 4 cut(s) 27, 130, 600, 703
XmnI GAANNNNTTC 3 cut(s) 1608, 2598, 2625
XspI CTAG 7 cut(s) 77, 650, 1121, 1533, 1865, 1919, 3066
ZraI GACGTC 1 cut(s) 2101
ZrmI AGTACT 2 cut(s) 2821, 3087
Zsp2I ATGCAT 2 cut(s) 1090, 2214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.