Rmu_sc0003226.1_g000025

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003226.1
OG0000105
Physical Location & Seq
Forward (+)
113739 .. 117539
3801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003226.1_g000025.1.cds

Sequence Viewer

Length: 3099 bp
atgcccgtcttctacaaagtggagccttctgatgtgcggcaccaaaggggaagttttgagctcaaagtgaaaacccgaccccgagtagaagtaagggaacatgaagaagtttatggcgagaatgaggacttcaaggcgtggagagctgctttgagagaaatagccgatctttctgggtgggattcaaagaattttagaactgagagagaacttgtagaggcaattgttggggatattgggagtaaggtggtgtataaatcttcaagtgttgacaaaggcttcgtaggaatggatgtccgtgttgatgttttattacataattacatagatcaagagttggatgaggtacgaattatagggatacatgggatgcgaggagttggtaagacagcgcttgctcgagctttttatgatgactttggtcaggattttgagcatagatgctttcttctcaatgttagagaaagctctaaaaggggtggcctagtttctttacaaaagaaccttctttcgagcatcatcttcatggacaaaattgatttcataaaggatgaatatacaggagctgatgtgattaaaagaagtttatgtaaaataaaggtgcttattgttattgatgatgtggatcagttggcacaattacgaaacttggctggaagtcgcgactggtttggttcagggagcagaattatcataaccaccacggataatcgcttgttagtggcacatggtgttgatgctacatacaatgctactgggttaaattctaatgaagctcttcaactattgagtttaaacgctttcaagaaaagcttcccaccagaaaattattcggacctgtgtgaccatattttagggtatgctgaggggctgccgttagctcttgtggttttaggttcttttctatgtggtagagagcccaatgaatgggcaagtgcaattgataggctaaaacatacaccaaatagaggaattatggatgtgcttcagattagttttgatggtctagaggacatggagaaagaaatattcctacatattgcttgtttttacaaggggaaagaccggcatcgtgtgacacaaatactagactattgtgacctgtaccctgacattggattaagtgttcttgctgataaatctctcattactacttccggcaatgaactgtcaatgcatgatttgctacaagaaatgggctgggaaatagttcgtcgagagtcttgtgatgagccaggcaaacgtagtagggtatggtctcatgaagacatccacaatatgttggagaaaaatgagggaacaaaagcaatccgaggcatggtaatggagttgcctaaattagaagtggctcattggaatcgagaagccttctcaaagttgtctcaccttagtcttctccatattcgtaatgtggaccttcccaaaggcctcacttgtctttctaattccttgagactcctggaatggcctgggtgtccgttaagatctctcccacaaaagttcgaaccagacaaacttattgaacttaacttgtgtcacagcaatattaaacgtctttggaaaggaacaaagaattttaaaaagttgaagttcatcaaactctgccattctcaaaacattgtggagaccccagacctcgcaggcgttcagaatcttgagagtttagatcttgaaggatgtaagagtttggtaagaatccatcaatcccttggatttctcaaaaatcttattgtcctgaatcttaaaggctgcaaaagtcttgagagtctgccaggtagagttgaaatggaatctcttgaaacattaattctttctaactgctcaaaaattaaaaagattcccgagtttgatggaaatatggagcatctgtcggtgctttatctcgacgagactgccattgaggaactgcctgtttcaattgaacgcctgactggccttgtgtcattgaatctaagtaactgcacaaatcttgtttgtcttccaagcaccatcaataaattgaagtctgttgaaaatcttaatctttctggatgcatgaaacttggcaaacatcaggagagcgtgggggaggtggactgttatgaggaaaatgatgtgaatagtgggtctctggcagaaatgtcatctacccgtgatctcataaactatgtgagaggtccaatcattcatagatgcaaagtgaatacagagccaatgagtttccgcttgcctatatggggtctctgtaatttaacatatctgaacctgagtaattgcaatcttggcgaaggagcatttgccaacgaatttggttactttccctctttggtgaccttgaatctaagtggaaacaattttgttcatcttccttcaggcattggattgctttctaagcttgagaactttaacttggagaattgcaagagacttgaagagttgtcagaccttccatcaaatggtagactagatttaagggcagatggttgtacttcactgaaatgcttgtttgatgcatcaaatttgaacagattaaacgaattatatttcagtttcatgaattgcgtcaatctaaatggcaatcaaggatgcaataacatagcatttgaaatgctgaggacattcatgtatcagggaatctctaatatgatggaaacttctcgaattgcaattcctggaagtaatattcctgagtggttcagccatcgaagtgttgggtgttcagtaagtgtggagcttcctccagattgggatagcagtaggtttatgggatttgctttgtgtattgtttttgtactactccatgagcaccatcaggttgttgagcgtgatattcatcaattcaagacttttaacgctactcatcatcttgtatgttgcctgaagctcagtggaagagaattagaagtatacggcagacagcctgcatttcgctttagtgaagaattaggcctggttgagtcagatcacctgtggctattctatgtatctcgtgataaatactttggtacagagtggtggcatagcagttgcagtcagcttgatttcttctttgaaaccagagggccaggtctgaaggtgaaggagtgtggagtccgtctgatatatgagcaagatgtgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1032

Amino Acids

117.07

Weight (kDa)

5.86

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 39
AccB7I CCANNNNNTGG 2 cut(s) 927, 2435
AccI GTMKAC 2 cut(s) 2440, 2886
AccII CGCG 1 cut(s) 663
AciI CCGC 2 cut(s) 37, 2203
AclWI GGATC 1 cut(s) 633
AcsI RAATTY 5 cut(s) 190, 763, 1573, 2285, 2497
AcuI CTGAAG 4 cut(s) 971, 2334, 2879, 3071
AdeI CACNNNGTG 1 cut(s) 731
AfaI GTAC 5 cut(s) 348, 1106, 2467, 2772, 2986
AfeI AGCGCT 1 cut(s) 393
AfiI CCNNNNNNNGG 6 cut(s) 927, 968, 1115, 1318, 2216, 2435
AjnI CCWGG 7 cut(s) 1234, 1458, 1468, 1771, 2650, 2928, 3043
AjuI GAANNNNNNNTTGG 2 cut(s) 2372, 2404
AloI GAACNNNNNNTCC 2 cut(s) 1110, 1142
Alw21I GWGCWC 2 cut(s) 63, 2787
Alw26I GTCTC 8 cut(s) 1263, 1386, 1447, 1619, 1883, 2112, 2225, 2398
AlwI GGATC 1 cut(s) 633
AlwNI CAGNNNCTG 1 cut(s) 566
Ama87I CYCGRG 3 cut(s) 81, 399, 1841
Aor51HI AGCGCT 1 cut(s) 393
AoxI GGCC 6 cut(s) 481, 1426, 1466, 1933, 2926, 3041
ApeKI GCWGC 3 cut(s) 146, 871, 1749
ApoI RAATTY 5 cut(s) 190, 763, 1573, 2285, 2497
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AseI ATTAAT 1 cut(s) 1805
Asp700I GAANNNNTTC 3 cut(s) 777, 812, 1209
AspLEI GCGC 1 cut(s) 394
AspS9I GGNCC 4 cut(s) 835, 1414, 2156, 3041
AsuHPI GGTGA 4 cut(s) 1376, 2320, 2936, 3067
AsuII TTCGAA 1 cut(s) 1503
AvaI CYCGRG 3 cut(s) 81, 399, 1841
AvaII GGWCC 3 cut(s) 835, 1414, 2156
BanI GGYRCC 1 cut(s) 39
BanII GRGCYC 2 cut(s) 63, 921
BauI CACGAG 1 cut(s) 2967
BbsI GAAGAC 3 cut(s) 1272, 1385, 1970
Bbv12I GWGCWC 2 cut(s) 63, 2787
BbvCI CCTCAGC 2 cut(s) 864, 2591
BbvI GCAGC 3 cut(s) 133, 858, 1736
BccI CCATC 9 cut(s) 995, 1707, 1844, 1997, 2437, 2453, 2620, 2688, 2796
BceAI ACGGC 2 cut(s) 859, 2905
BcgI CGANNNNNNTGC 2 cut(s) 1874, 1908
BciT130I CCWGG 7 cut(s) 1236, 1460, 1470, 1773, 2652, 2930, 3045
BciVI GTATCC 1 cut(s) 354
BcoDI GTCTC 8 cut(s) 1263, 1386, 1447, 1619, 1883, 2112, 2225, 2398
BfaI CTAG 4 cut(s) 485, 1007, 1088, 2444
BfoI RGCGCY 1 cut(s) 395
BfuI GTATCC 1 cut(s) 354
BglI GCCNNNNNGGC 1 cut(s) 1932
BglII AGATCT 2 cut(s) 1484, 1666
BisI GCNGC 4 cut(s) 38, 147, 872, 1750
BlsI GCNGC 4 cut(s) 39, 148, 873, 1751
Bme1390I CCNGG 7 cut(s) 1236, 1460, 1470, 1773, 2652, 2930, 3045
Bme18I GGWCC 3 cut(s) 835, 1414, 2156
BmeT110I CYCGRG 3 cut(s) 81, 399, 1841
BmgT120I GGNCC 4 cut(s) 835, 1414, 2156, 3041
BmiI GGNNCC 2 cut(s) 24, 41
BmrFI CCNGG 7 cut(s) 1236, 1460, 1470, 1773, 2652, 2930, 3045
BmrI ACTGGG 1 cut(s) 765
BmuI ACTGGG 1 cut(s) 765
BoxI GACNNNNGTC 1 cut(s) 420
BpiI GAAGAC 3 cut(s) 1272, 1385, 1970
BpmI CTGGAG 1 cut(s) 2703
Bpu10I CCTNAGC 2 cut(s) 864, 2591
Bpu14I TTCGAA 1 cut(s) 1503
BpuEI CTTGAG 4 cut(s) 1471, 1676, 1781, 2396
BsaBI GATNNNNATC 2 cut(s) 624, 2811
BsaI GGTCTC 4 cut(s) 1263, 1619, 2112, 2225
BsaJI CCNNGG 4 cut(s) 702, 1312, 1469, 1708
BsaXI ACNNNNNCTCC 4 cut(s) 555, 585, 1853, 1883
Bsc4I CCNNNNNNNGG 6 cut(s) 927, 968, 1115, 1318, 2216, 2435
Bse118I RCCGGY 1 cut(s) 1065
Bse1I ACTGG 3 cut(s) 671, 760, 1936
Bse3DI GCAATG 1 cut(s) 1168
Bse8I GATNNNNATC 2 cut(s) 624, 2811
BseBI CCWGG 7 cut(s) 1236, 1460, 1470, 1773, 2652, 2930, 3045
BseDI CCNNGG 4 cut(s) 702, 1312, 1469, 1708
BseGI GGATG 9 cut(s) 298, 346, 375, 556, 985, 1269, 1682, 2036, 2570
BseJI GATNNNNATC 2 cut(s) 624, 2811
BseLI CCNNNNNNNGG 6 cut(s) 927, 968, 1115, 1318, 2216, 2435
BseMI GCAATG 1 cut(s) 1168
BseMII CTCAG 6 cut(s) 192, 855, 2237, 2582, 2658, 2878
BseNI ACTGG 3 cut(s) 671, 760, 1936
BseRI GAGGAG 1 cut(s) 390
BseXI GCAGC 3 cut(s) 133, 858, 1736
BseYI CCCAGC 1 cut(s) 1200
BsgI GTGCAG 1 cut(s) 1945
Bsh1236I CGCG 1 cut(s) 663
BshFI GGCC 6 cut(s) 483, 1428, 1468, 1935, 2928, 3043
BshNI GGYRCC 1 cut(s) 39
BsiHKAI GWGCWC 2 cut(s) 63, 2787
BsiHKCI CYCGRG 3 cut(s) 81, 399, 1841
BsiSI CCGG 2 cut(s) 1066, 1158
BslI CCNNNNNNNGG 6 cut(s) 927, 968, 1115, 1318, 2216, 2435
BsmAI GTCTC 8 cut(s) 1263, 1386, 1447, 1619, 1883, 2112, 2225, 2398
BsnI GGCC 6 cut(s) 483, 1428, 1468, 1935, 2928, 3043
Bso31I GGTCTC 4 cut(s) 1263, 1619, 2112, 2225
BsoBI CYCGRG 3 cut(s) 81, 399, 1841
Bsp119I TTCGAA 1 cut(s) 1503
Bsp1286I GDGCHC 3 cut(s) 63, 921, 2787
Bsp143I GATC 7 cut(s) 166, 328, 625, 1484, 1666, 2134, 2941
Bsp68I TCGCGA 1 cut(s) 663
BspACI CCGC 2 cut(s) 37, 2203
BspANI GGCC 6 cut(s) 483, 1428, 1468, 1935, 2928, 3043
BspCNI CTCAG 6 cut(s) 193, 856, 2238, 2583, 2659, 2877
BspFNI CGCG 1 cut(s) 663
BspHI TCATGA 2 cut(s) 1261, 2532
BspLI GGNNCC 2 cut(s) 24, 41
BspPI GGATC 1 cut(s) 633
BspQI GCTCTTC 1 cut(s) 783
BspT104I TTCGAA 1 cut(s) 1503
BspT107I GGYRCC 1 cut(s) 39
BspTNI GGTCTC 4 cut(s) 1263, 1619, 2112, 2225
BsrDI GCAATG 1 cut(s) 1168
BsrFI RCCGGY 1 cut(s) 1065
BsrI ACTGG 3 cut(s) 671, 760, 1936
BssAI RCCGGY 1 cut(s) 1065
BssECI CCNNGG 4 cut(s) 702, 1312, 1469, 1708
BssMI GATC 7 cut(s) 166, 328, 625, 1484, 1666, 2134, 2941
BssNAI GTATAC 1 cut(s) 2887
BssSI CACGAG 1 cut(s) 2967
BssT1I CCWWGG 1 cut(s) 1708
Bst1107I GTATAC 1 cut(s) 2887
Bst2BI CACGAG 1 cut(s) 2967
Bst2UI CCWGG 7 cut(s) 1236, 1460, 1470, 1773, 2652, 2930, 3045
Bst4CI ACNGT 2 cut(s) 1170, 2078
Bst6I CTCTTC 3 cut(s) 783, 2406, 2866
BstAPI GCANNNNNTGC 1 cut(s) 1183
BstBI TTCGAA 1 cut(s) 1503
BstC8I GCNNGC 4 cut(s) 396, 1642, 2207, 2901
BstDSI CCRYGG 1 cut(s) 702
BstEII GGTNACC 1 cut(s) 2308
BstF5I GGATG 9 cut(s) 298, 346, 375, 556, 985, 1269, 1682, 2036, 2570
BstFNI CGCG 1 cut(s) 663
BstH2I RGCGCY 1 cut(s) 395
BstHHI GCGC 1 cut(s) 394
BstKTI GATC 7 cut(s) 169, 331, 628, 1487, 1669, 2137, 2944
BstMAI GTCTC 8 cut(s) 1263, 1386, 1447, 1619, 1883, 2112, 2225, 2398
BstMBI GATC 7 cut(s) 166, 328, 625, 1484, 1666, 2134, 2941
BstMWI GCNNNNNNNGC 5 cut(s) 143, 1183, 1932, 2262, 2371
BstNI CCWGG 7 cut(s) 1236, 1460, 1470, 1773, 2652, 2930, 3045
BstPAI GACNNNNGTC 1 cut(s) 420
BstPI GGTNACC 1 cut(s) 2308
BstSCI CCNGG 7 cut(s) 1234, 1458, 1468, 1771, 2650, 2928, 3043
BstUI CGCG 1 cut(s) 663
BstV1I GCAGC 3 cut(s) 133, 858, 1736
BstV2I GAAGAC 3 cut(s) 1272, 1385, 1970
BstX2I RGATCY 2 cut(s) 1484, 1666
BstYI RGATCY 2 cut(s) 1484, 1666
BstZ17I GTATAC 1 cut(s) 2887
BsuI GTATCC 1 cut(s) 354
BsuRI GGCC 6 cut(s) 483, 1428, 1468, 1935, 2928, 3043
BtgI CCRYGG 1 cut(s) 702
BtsCI GGATG 9 cut(s) 298, 346, 375, 556, 985, 1269, 1682, 2036, 2570
BtsIMutI CAGTG 2 cut(s) 2471, 2872
BtuMI TCGCGA 1 cut(s) 663
Cac8I GCNNGC 4 cut(s) 396, 1642, 2207, 2901
CaiI CAGNNNCTG 1 cut(s) 566
CciI TCATGA 2 cut(s) 1261, 2532
CfoI GCGC 1 cut(s) 394
Cfr10I RCCGGY 1 cut(s) 1065
Cfr13I GGNCC 4 cut(s) 835, 1414, 2156, 3041
CseI GACGC 1 cut(s) 2530
Csp6I GTAC 5 cut(s) 347, 1105, 2466, 2771, 2985
CviQI GTAC 5 cut(s) 347, 1105, 2466, 2771, 2985
DpnI GATC 7 cut(s) 168, 330, 627, 1486, 1668, 2136, 2943
DpnII GATC 7 cut(s) 166, 328, 625, 1484, 1666, 2134, 2941
DraI TTTAAA 2 cut(s) 795, 1579
DraIII CACNNNGTG 1 cut(s) 731
Eam1104I CTCTTC 3 cut(s) 783, 2406, 2866
EarI CTCTTC 3 cut(s) 783, 2406, 2866
Ecl136II GAGCTC 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 1708
Eco147I AGGCCT 2 cut(s) 1428, 2928
Eco24I GRGCYC 2 cut(s) 63, 921
Eco31I GGTCTC 4 cut(s) 1263, 1619, 2112, 2225
Eco47I GGWCC 3 cut(s) 835, 1414, 2156
Eco47III AGCGCT 1 cut(s) 393
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 4 cut(s) 971, 2334, 2879, 3071
Eco88I CYCGRG 3 cut(s) 81, 399, 1841
Eco91I GGTNACC 1 cut(s) 2308
EcoICRI GAGCTC 1 cut(s) 61
EcoO65I GGTNACC 1 cut(s) 2308
EcoRII CCWGG 7 cut(s) 1234, 1458, 1468, 1771, 2650, 2928, 3043
EcoT14I CCWWGG 1 cut(s) 1708
EcoT22I ATGCAT 3 cut(s) 1179, 2036, 2494
EcoT38I GRGCYC 2 cut(s) 63, 921
ErhI CCWWGG 1 cut(s) 1708
FalI AAGNNNNNCTT 2 cut(s) 797, 829
FblI GTMKAC 2 cut(s) 2440, 2886
Fnu4HI GCNGC 4 cut(s) 38, 147, 872, 1750
FokI GGATG 9 cut(s) 305, 353, 382, 563, 992, 1256, 1689, 2043, 2577
FriOI GRGCYC 2 cut(s) 63, 921
Fsp4HI GCNGC 4 cut(s) 38, 147, 872, 1750
FspBI CTAG 4 cut(s) 485, 1007, 1088, 2444
GlaI GCGC 1 cut(s) 393
GluI GCNGC 4 cut(s) 38, 147, 872, 1750
GsaI CCCAGC 1 cut(s) 1204
GsuI CTGGAG 1 cut(s) 2703
HaeII RGCGCY 1 cut(s) 395
HaeIII GGCC 6 cut(s) 483, 1428, 1468, 1935, 2928, 3043
HapII CCGG 2 cut(s) 1066, 1158
HgaI GACGC 1 cut(s) 2530
HhaI GCGC 1 cut(s) 394
Hin6I GCGC 1 cut(s) 392
HinP1I GCGC 1 cut(s) 392
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 2 cut(s) 811, 2372
HpaII CCGG 2 cut(s) 1066, 1158
HphI GGTGA 4 cut(s) 1376, 2320, 2936, 3067
Hpy166II GTNNAC 5 cut(s) 271, 1414, 2074, 2441, 2887
Hpy8I GTNNAC 5 cut(s) 271, 1414, 2074, 2441, 2887
Hpy99I CGWCG 2 cut(s) 1218, 1889
HpyCH4III ACNGT 2 cut(s) 1170, 2078
HpyCH4IV ACGT 2 cut(s) 1243, 1552
HpyF10VI GCNNNNNNNGC 5 cut(s) 143, 1183, 1932, 2262, 2371
HpySE526I ACGT 2 cut(s) 1243, 1552
HspAI GCGC 1 cut(s) 392
Kzo9I GATC 7 cut(s) 166, 328, 625, 1484, 1666, 2134, 2941
LguI GCTCTTC 1 cut(s) 783
LmnI GCTCC 6 cut(s) 22, 563, 681, 1861, 2270, 2710
Lsp1109I GCAGC 3 cut(s) 133, 858, 1736
MaeI CTAG 4 cut(s) 485, 1007, 1088, 2444
MaeII ACGT 2 cut(s) 1243, 1552
MaeIII GTNAC 7 cut(s) 842, 1075, 1097, 1535, 1955, 2291, 2308
MalI GATC 7 cut(s) 168, 330, 627, 1486, 1668, 2136, 2943
MboI GATC 7 cut(s) 166, 328, 625, 1484, 1666, 2134, 2941
MfeI CAATTG 3 cut(s) 222, 939, 1917
MflI RGATCY 2 cut(s) 1484, 1666
MhlI GDGCHC 3 cut(s) 63, 921, 2787
MlyI GAGTC 5 cut(s) 1229, 1449, 1774, 2945, 3078
MmeI TCCRAC 2 cut(s) 318, 1263
Mph1103I ATGCAT 3 cut(s) 1179, 2036, 2494
MroXI GAANNNNTTC 3 cut(s) 777, 812, 1209
MslI CAYNNNNRTG 4 cut(s) 524, 1322, 2476, 2685
MspI CCGG 2 cut(s) 1066, 1158
MspR9I CCNGG 7 cut(s) 1236, 1460, 1470, 1773, 2652, 2930, 3045
MssI GTTTAAAC 1 cut(s) 795
MunI CAATTG 3 cut(s) 222, 939, 1917
MvaI CCWGG 7 cut(s) 1236, 1460, 1470, 1773, 2652, 2930, 3045
MvnI CGCG 1 cut(s) 663
MwoI GCNNNNNNNGC 5 cut(s) 143, 1183, 1932, 2262, 2371
NdeII GATC 7 cut(s) 166, 328, 625, 1484, 1666, 2134, 2941
NlaIV GGNNCC 2 cut(s) 24, 41
NmuCI GTSAC 5 cut(s) 842, 1075, 1097, 1535, 2308
NruI TCGCGA 1 cut(s) 663
NsiI ATGCAT 3 cut(s) 1179, 2036, 2494
NspV TTCGAA 1 cut(s) 1503
PaeR7I CTCGAG 1 cut(s) 399
PagI TCATGA 2 cut(s) 1261, 2532
PceI AGGCCT 2 cut(s) 1428, 2928
PciSI GCTCTTC 1 cut(s) 783
PdmI GAANNNNTTC 3 cut(s) 777, 812, 1209
PflMI CCANNNNNTGG 2 cut(s) 927, 2435
PfoI TCCNGGA 2 cut(s) 1458, 2650
PkrI GCNGC 4 cut(s) 39, 148, 873, 1751
PleI GAGTC 5 cut(s) 1228, 1449, 1773, 2944, 3077
PmeI GTTTAAAC 1 cut(s) 795
PpsI GAGTC 5 cut(s) 1228, 1449, 1773, 2944, 3077
PshAI GACNNNNGTC 1 cut(s) 420
PshBI ATTAAT 1 cut(s) 1805
Psp124BI GAGCTC 1 cut(s) 63
Psp6I CCWGG 7 cut(s) 1234, 1458, 1468, 1771, 2650, 2928, 3043
PspEI GGTNACC 1 cut(s) 2308
PspFI CCCAGC 1 cut(s) 1200
PspGI CCWGG 7 cut(s) 1234, 1458, 1468, 1771, 2650, 2928, 3043
PspN4I GGNNCC 2 cut(s) 24, 41
PspPI GGNCC 4 cut(s) 835, 1414, 2156, 3041
PspXI VCTCGAGB 1 cut(s) 399
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 2 cut(s) 1484, 1666
RruI TCGCGA 1 cut(s) 663
RsaI GTAC 5 cut(s) 348, 1106, 2467, 2772, 2986
RsaNI GTAC 5 cut(s) 347, 1105, 2466, 2771, 2985
RseI CAYNNNNRTG 4 cut(s) 524, 1322, 2476, 2685
SacI GAGCTC 1 cut(s) 63
SapI GCTCTTC 1 cut(s) 783
SatI GCNGC 4 cut(s) 38, 147, 872, 1750
Sau3AI GATC 7 cut(s) 166, 328, 625, 1484, 1666, 2134, 2941
Sau96I GGNCC 4 cut(s) 835, 1414, 2156, 3041
SchI GAGTC 5 cut(s) 1229, 1449, 1774, 2945, 3078
ScrFI CCNGG 7 cut(s) 1236, 1460, 1470, 1773, 2652, 2930, 3045
SduI GDGCHC 3 cut(s) 63, 921, 2787
Sfr274I CTCGAG 1 cut(s) 399
SfuI TTCGAA 1 cut(s) 1503
SinI GGWCC 3 cut(s) 835, 1414, 2156
SlaI CTCGAG 1 cut(s) 399
SmiMI CAYNNNNRTG 4 cut(s) 524, 1322, 2476, 2685
SmlI CTYRAG 5 cut(s) 399, 1450, 1655, 1760, 2375
SmoI CTYRAG 5 cut(s) 399, 1450, 1655, 1760, 2375
SseBI AGGCCT 2 cut(s) 1428, 2928
SsiI CCGC 2 cut(s) 37, 2203
SspI AATATT 3 cut(s) 1029, 1546, 2662
SspMI CTAG 4 cut(s) 485, 1007, 1088, 2444
SstI GAGCTC 1 cut(s) 63
StuI AGGCCT 2 cut(s) 1428, 2928
StyD4I CCNGG 7 cut(s) 1234, 1458, 1468, 1771, 2650, 2928, 3043
StyI CCWWGG 1 cut(s) 1708
TaaI ACNGT 2 cut(s) 1170, 2078
TaiI ACGT 2 cut(s) 1246, 1555
TaqI TCGA 8 cut(s) 400, 512, 1216, 1360, 1503, 1884, 2638, 2683
TatI WGTACW 2 cut(s) 2465, 2770
TauI GCSGC 1 cut(s) 40
TscAI CASTG 2 cut(s) 2478, 2872
TseFI GTSAC 5 cut(s) 842, 1075, 1097, 1535, 2308
TseI GCWGC 3 cut(s) 146, 871, 1749
Tsp45I GTSAC 5 cut(s) 842, 1075, 1097, 1535, 2308
TspGWI ACGGA 4 cut(s) 287, 719, 1467, 3062
TspRI CASTG 2 cut(s) 2478, 2872
Van91I CCANNNNNTGG 2 cut(s) 927, 2435
VpaK11BI GGWCC 3 cut(s) 835, 1414, 2156
VspI ATTAAT 1 cut(s) 1805
XapI RAATTY 5 cut(s) 190, 763, 1573, 2285, 2497
XbaI TCTAGA 1 cut(s) 1006
XcmI CCANNNNNNNNNTGG 2 cut(s) 1706, 2687
XhoI CTCGAG 1 cut(s) 399
XmiI GTMKAC 2 cut(s) 2440, 2886
XmnI GAANNNNTTC 3 cut(s) 777, 812, 1209
XspI CTAG 4 cut(s) 485, 1007, 1088, 2444
Zsp2I ATGCAT 3 cut(s) 1179, 2036, 2494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.