Prupe.8G005200_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000105
Physical Location & Seq
Forward (+)
403391 .. 408286
4896 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G005200.1

Sequence Viewer

Length: 3387 bp
ATGGCCGCCCAAACGGCCCCCTCTCCATATACTTCTCCCTCTTCTTCCTTGAACGGGCGTACATGGAAATACGACGTGTTCCTCAGTTTCAGAGGCGAAGATACGCGGAATACTTTCACAGACCATCTATATCATGCTCTCCTTCAGAGAAGAGTGATCGTATATAGAGATAATGAACTTAAGCGGGGAGACAACATTTCCCAAGTAGTCTACAAGGCACTTGAACAATCACGAATTTCGATCGTCATTCTTTCAAGTAATTATGCCAATTCAAAGTGGTGCTTGGATGAGCTATCGAAGATTGTCGAGTGCATGAATGGGATGAGACAGAGAGTTCTCCCAGTTTTCTATGATGTGGAGCCATCTGAGGTACGAAAACAGACGGGGACTTTCGGGAACGCATTTGCTGAGCACGAACAAGTTTTCAGAGACAATAGAGAGAAGGTGCTTCGTTGGAGGGATGCTCTTTATCAAGTAGCCAACCTCTCCGGATTTGTGATAAGAAATCGGTATGAATCCGAAGTTATCAGTCAAATTCTTAAAATGGTACTGAATGCATTGCCACAGGTGTTTTCTCATGGCTTCCTAGTGGGAATTGATTCAAGAGTAGATGAAATACATGTGCTCTTGGATTTAGAATCAAATGATGTTCGCTTCATAGGGATATGGGGGATGGGTGGTATAGGAAAGACAACTATCGCTGAAGTAATTTTTCAAAAAATTTCGGCTGAATTTGAAATTTTCACCTATGTCCCCAATATTAGGGAGGCAACTAACGAACAGGGTGGTCTACTTCAACTGCAAAAGAATCTTCTTTCTGAGGCTTTAATGCAAATTAACCTAGATGTTCTAAGTGTTGCTGAGGGAGCCAGAATGATAAGGAACAGTCTATCTAATAGAAAGGTTCTTCTCTTTTTGGACGATGTGGATCATTTAGATCAACTAGAATCATTGGCTGGAAACCAAAATTGGTTTGGTTTGGGGAGCAGAGTCATCATTACGACTAGAAATGAGAAATTGCTAAGAGACCATGGTGTAGATAACATATTTGAAGTTGGGGAATTAAAGGACAATGAATCTCTTCAGCTTTTCAGTTATGGCGCCTTCAAAAGTCATAAACCTCCAGAAGATTATCTGGATTTGTCTAAACTGGTTGTTAACTATGCTAGAGGCATTCCCTTGGCACTTGTGGTTTTGGGTTCCTTTCTGATGGGCAGAAATGTGACTGAGTGGATAAGTGTATTGCAAAGACTAAAGGAACTTCCTCATAGAGAAGTTTTTGATGTACTCAAAATAAGTTATGACGGATTACAGAACAACGAGAAAAGAATTTTTCTAGATATTGCTTGTTTCCTTAAAGGAATGGACAAAGAGCGAGTGGAAGAAATACTAGATTATTTTGGTTTTAATCCGAAGGTTGGAATACAAGTTCTCATTGAGAAATCTCTCATAACTATTTTAAATAACAAAGTGTTGATGAATGGATTCATACAAGAAATGGGACAACAACTTGTACGCAGAGAGTATGTTGACGAGCCTGGAAAGCGAAGCAGGTTGTGGCTTTTTGATGACATCATTTATGTACTGAACAATAATAAGGGAACAAATGCAGTTGAAGGTATAGCCTTAGACTTGCCTAAACTAAAAGTGGCATGCTGGAACTCGGAGTCCTTCTCAAACATGCAAAATCTTAGATTTCTCAAAATTCATAATCTGCAGATGACCCAAGGCCCGGAATATCTTTCTAATGCCTTGAAATTTCTTGAATGGAGCGGATATCCTTCAAAATTTCTCCCACAAGGATTTCAACCTGAGGAACTTTGTGAGCTTAACTTGTGCCATAGCAGCATTGGACAGCTTTGGAGGGGAACAAAGTGCTTAGGCAATTTGAAATCCATCAACGTTAGCTACTCCCAGAATCTGACCAGGACCCCAGATTTTACAGTTACTCCAAATCTTCGTAGATTAATTCTTGAAGGTTGTACAAATTTGGTGGAAATCCACCAATCCATTGGTGAGCTCAAAAGGCTTATATTCTTGAATCTTAAAGATTGCAGAAGGCTTGGACATCTTCCGGATGATTTGCAAACAGAAAGTCTTAAAGTCCTTATTCTTTCTGGGTGTCCAAATATTAAGAAAATCCCAGTATTTTGGAGACCTATGGAACACTTGTCGGAACTTTTTTTTGATAAGACTGCTATAGAAGAATTACCTACTTCAGTTGAATGTCTGACTGGCCTTACTTTATTAAATCTAAGAGATTGTAGACATCTCAATGGTCTTACAAGCAATATTTGCAAGTTGAGGTCCCTCAAAAGTCTTAATCTCAGTGGATGCTCAAAACTTGGAAAGCTGCCAGAGAACTTGGGTAAGATAGATTGTTTGGAGGAGCTTGATGCTTGTGGTACGGCCATAAGTGCTCTACCATCCTCCATTTCCCGTCTGGAAAATCTTAAAGGGTTATCACTTTGTGGATGTAAATGGATGCCAAGAAAGAGGACGAGGTCATTGGGTTTGCTGTTGCCAAACACTGATTCTGGTTTGCGTTGTTTAACACTACTGAATCTCAGTGACTGTAATCTTCAGGAGGTAACAATCCTGGAAAATCTTGGCTGCTTATCCTCATTAGTATCACTAAATCTCAGTAAAAACAATTTTGTTACTCTTCCCAAAAGCATCAGACAGCTGTCCAAGCTTCAAAATTTGAATTTGGGTAGTTGTAAGAGTCTTCAAAAATTGCCGGACCTTTCAGAAAAACTGAACTTCAGTGTTGGGGCAGAAGGCAGTTATTCACAGGAAAGGCTGTCATCATGTTTCAGTTTTATTAATTGCTCCAAAGTGGTTGACAATCAAGGCTGCAAAAATATGGCATTTGCAATGTTAAGGAGATCCATAGAGGGAATCCCTTATGCAGGGAACAGATTTGAAGCTATATTTCCTGGCAGTAAAATTTCTGAGTGGTTCAGTTATCACAATGAGGGACCTGTAGTAAGCATGGACCTCCCCCGCCACTGGTATAAGAACAAGTGGATGGGATATGCTTTGTGTGCTGTTTTTGTTCTTCGTCGACGTCACCCACCTAACCTGCTTGGTAAATGGAAGTATGGGACCCATAGCACCGCACATGGTCTTCGTTGTGAAGTGAAGCCTGGGAATTTGGGCATAGGTGGCCGCTGTCCTTTCTTTGGCTGCAGCGAAGAGTTAGGCCAAATTGAGTCGGAACACATTTGGCTGTCCTTTGTATCCGGTGAATATTTTGGTACATCATGGCAAGACAGCTGTCGTCACCTTGAGTTTTCATTTAAATCCTTAGGCCCTGGCTTGGAGTTGAAGAGGTGTGGAGTCCGTTTCCTATATGAGAAAGATTTGGTTGGAATCAAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1129

Amino Acids

127.98

Weight (kDa)

8.43

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 3073
Acc36I ACCTGC 2 cut(s) 1542, 3093
AccB1I GGYRCC 1 cut(s) 1100
AccBSI CCGCTC 1 cut(s) 1773
AccI GTMKAC 4 cut(s) 210, 790, 2266, 3067
AccII CGCG 1 cut(s) 106
AccIII TCCGGA 2 cut(s) 488, 2074
AciI CCGC 7 cut(s) 6, 106, 184, 1773, 3007, 3120, 3172
AclI AACGTT 1 cut(s) 1902
AclWI GGATC 2 cut(s) 936, 2883
AcoI YGGCCR 3 cut(s) 3, 2409, 3169
AcuI CTGAAG 6 cut(s) 128, 723, 1067, 2202, 2567, 2749
AcyI GRCGYC 2 cut(s) 1101, 3070
AdeI CACNNNGTG 1 cut(s) 2471
AfaI GTAC 9 cut(s) 61, 372, 549, 1287, 1515, 1584, 1984, 2407, 3262
AfiI CCNNNNNNNGG 8 cut(s) 54, 762, 1418, 1732, 2912, 3012, 3185, 3322
AflII CTTAAG 1 cut(s) 179
AflIII ACRYGT 2 cut(s) 75, 619
AjiI CACGTC 1 cut(s) 76
AjnI CCWGG 6 cut(s) 1537, 1925, 2598, 2938, 3148, 3316
AjuI GAANNNNNNNTTGG 4 cut(s) 952, 984, 2518, 2550
AloI GAACNNNNNNTCC 4 cut(s) 1413, 1445, 1652, 1684
Alw21I GWGCWC 4 cut(s) 414, 627, 2022, 2422
Alw26I GTCTC 5 cut(s) 183, 319, 423, 1020, 2149
AlwI GGATC 2 cut(s) 936, 2883
AlwNI CAGNNNCTG 2 cut(s) 1921, 2574
Aor13HI TCCGGA 2 cut(s) 488, 2074
AoxI GGCC 8 cut(s) 3, 15, 1729, 2236, 2409, 3169, 3205, 3313
ApeKI GCWGC 6 cut(s) 1845, 2353, 2613, 2856, 3189, 3192
ArsI GACNNNNNNTTYG 2 cut(s) 2080, 2112
AseI ATTAAT 2 cut(s) 1967, 2826
Asp700I GAANNNNTTC 4 cut(s) 113, 598, 1080, 1484
AspLEI GCGC 1 cut(s) 1103
AspS9I GGNCC 9 cut(s) 16, 1730, 1929, 2307, 2743, 2981, 2998, 3108, 3314
AsuC2I CCSGG 1 cut(s) 1733
AsuHPI GGTGA 5 cut(s) 736, 2027, 3065, 3260, 3278
AvaII GGWCC 6 cut(s) 1929, 2307, 2743, 2981, 2998, 3108
AxyI CCTNAGG 2 cut(s) 1812, 3310
BanI GGYRCC 1 cut(s) 1100
BanII GRGCYC 1 cut(s) 2022
BbsI GAAGAC 2 cut(s) 2720, 3122
Bbv12I GWGCWC 4 cut(s) 414, 627, 2022, 2422
BbvCI CCTCAGC 1 cut(s) 861
BbvI GCAGC 6 cut(s) 1857, 2340, 2600, 2843, 3176, 3204
BccI CCATC 7 cut(s) 132, 370, 667, 1204, 1904, 2434, 3025
BceAI ACGGC 2 cut(s) 30, 2424
BciT130I CCWGG 6 cut(s) 1539, 1927, 2600, 2940, 3150, 3318
BciVI GTATCC 1 cut(s) 3253
BcnI CCSGG 1 cut(s) 1733
BcoDI GTCTC 5 cut(s) 183, 319, 423, 1020, 2149
BfaI CTAG 7 cut(s) 587, 842, 944, 1005, 1167, 1337, 1391
BfmI CTRYAG 4 cut(s) 1715, 2199, 2985, 3190
BfoI RGCGCY 1 cut(s) 1104
BfrI CTTAAG 1 cut(s) 179
BfuAI ACCTGC 2 cut(s) 1542, 3093
BfuI GTATCC 1 cut(s) 3253
BglI GCCNNNNNGGC 1 cut(s) 14
BisI GCNGC 8 cut(s) 6, 1846, 2354, 2614, 2857, 3172, 3190, 3193
BlpI GCTNAGC 1 cut(s) 408
BlsI GCNGC 8 cut(s) 7, 1847, 2355, 2615, 2858, 3173, 3191, 3194
Bme1390I CCNGG 7 cut(s) 1539, 1733, 1927, 2600, 2940, 3150, 3318
Bme18I GGWCC 6 cut(s) 1929, 2307, 2743, 2981, 2998, 3108
BmgBI CACGTC 1 cut(s) 76
BmgT120I GGNCC 9 cut(s) 16, 1730, 1929, 2307, 2743, 2981, 2998, 3108, 3314
BmrFI CCNGG 7 cut(s) 1539, 1733, 1927, 2600, 2940, 3150, 3318
BmrI ACTGGG 2 cut(s) 335, 2138
BmsI GCATC 5 cut(s) 451, 2324, 2386, 2475, 2685
BmuI ACTGGG 2 cut(s) 335, 2138
BoxI GACNNNNGTC 2 cut(s) 2686, 3279
BpiI GAAGAC 2 cut(s) 2720, 3122
BplI GAGNNNNNCTC 4 cut(s) 448, 480, 1658, 1690
BpmI CTGGAG 1 cut(s) 1107
Bpu10I CCTNAGC 2 cut(s) 861, 1879
Bpu1102I GCTNAGC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 3311
BpuMI CCSGG 1 cut(s) 1733
BsaBI GATNNNNATC 1 cut(s) 927
BsaHI GRCGYC 2 cut(s) 1101, 3070
BsaI GGTCTC 2 cut(s) 1020, 2149
BsaJI CCNNGG 5 cut(s) 1030, 1179, 1726, 3149, 3316
BsaWI WCCGGW 3 cut(s) 488, 2074, 3245
BsaXI ACNNNNNCTCC 2 cut(s) 1933, 1963
Bsc4I CCNNNNNNNGG 8 cut(s) 54, 762, 1418, 1732, 2912, 3012, 3185, 3322
Bse1I ACTGG 5 cut(s) 341, 1155, 2144, 2239, 3017
Bse21I CCTNAGG 2 cut(s) 1812, 3310
Bse3DI GCAATG 2 cut(s) 557, 2883
Bse8I GATNNNNATC 1 cut(s) 927
BseAI TCCGGA 2 cut(s) 488, 2074
BseBI CCWGG 6 cut(s) 1539, 1927, 2600, 2940, 3150, 3318
BseDI CCNNGG 5 cut(s) 1030, 1179, 1726, 3149, 3316
BseJI GATNNNNATC 1 cut(s) 927
BseLI CCNNNNNNNGG 8 cut(s) 54, 762, 1418, 1732, 2912, 3012, 3185, 3322
BseMI GCAATG 2 cut(s) 557, 2883
BseNI ACTGG 5 cut(s) 341, 1155, 2144, 2239, 3017
BseRI GAGGAG 1 cut(s) 2402
BseXI GCAGC 6 cut(s) 1857, 2340, 2600, 2843, 3176, 3204
Bsh1236I CGCG 1 cut(s) 106
Bsh1285I CGRYCG 1 cut(s) 243
BshFI GGCC 8 cut(s) 5, 17, 1731, 2238, 2411, 3171, 3207, 3315
BshNI GGYRCC 1 cut(s) 1100
BsiEI CGRYCG 1 cut(s) 243
BsiHKAI GWGCWC 4 cut(s) 414, 627, 2022, 2422
BsiSI CCGG 5 cut(s) 489, 1733, 2075, 2741, 3246
BslFI GGGAC 6 cut(s) 400, 737, 1515, 2293, 2994, 3121
BslI CCNNNNNNNGG 8 cut(s) 54, 762, 1418, 1732, 2912, 3012, 3185, 3322
BsmAI GTCTC 5 cut(s) 183, 319, 423, 1020, 2149
BsmFI GGGAC 6 cut(s) 400, 737, 1515, 2293, 2994, 3121
BsmI GAATGC 2 cut(s) 559, 1173
BsnI GGCC 8 cut(s) 5, 17, 1731, 2238, 2411, 3171, 3207, 3315
Bso31I GGTCTC 2 cut(s) 1020, 2149
Bsp1286I GDGCHC 4 cut(s) 414, 627, 2022, 2422
Bsp13I TCCGGA 2 cut(s) 488, 2074
Bsp1407I TGTACA 1 cut(s) 1982
Bsp143I GATC 5 cut(s) 156, 240, 928, 937, 2888
Bsp1720I GCTNAGC 1 cut(s) 408
Bsp19I CCATGG 1 cut(s) 1030
BspACI CCGC 7 cut(s) 6, 106, 184, 1773, 3007, 3120, 3172
BspANI GGCC 8 cut(s) 5, 17, 1731, 2238, 2411, 3171, 3207, 3315
BspEI TCCGGA 2 cut(s) 488, 2074
BspFNI CGCG 1 cut(s) 106
BspMAI CTGCAG 2 cut(s) 1719, 3194
BspMI ACCTGC 2 cut(s) 1542, 3093
BspPI GGATC 2 cut(s) 936, 2883
BspT107I GGYRCC 1 cut(s) 1100
BspTI CTTAAG 1 cut(s) 179
BspTNI GGTCTC 2 cut(s) 1020, 2149
BsrBI CCGCTC 1 cut(s) 1773
BsrDI GCAATG 2 cut(s) 557, 2883
BsrGI TGTACA 1 cut(s) 1982
BsrI ACTGG 5 cut(s) 341, 1155, 2144, 2239, 3017
BssECI CCNNGG 5 cut(s) 1030, 1179, 1726, 3149, 3316
BssMI GATC 5 cut(s) 156, 240, 928, 937, 2888
BssNI GRCGYC 2 cut(s) 1101, 3070
BssT1I CCWWGG 3 cut(s) 1030, 1179, 1726
Bst2UI CCWGG 6 cut(s) 1539, 1927, 2600, 2940, 3150, 3318
Bst4CI ACNGT 4 cut(s) 887, 1945, 2576, 3384
Bst6I CTCTTC 6 cut(s) 46, 145, 1086, 2670, 3192, 3326
BstACI GRCGYC 2 cut(s) 1101, 3070
BstAFI CTTAAG 1 cut(s) 179
BstAPI GCANNNNNTGC 1 cut(s) 2295
BstAUI TGTACA 1 cut(s) 1982
BstC8I GCNNGC 1 cut(s) 1654
BstDSI CCRYGG 1 cut(s) 1030
BstENI CCTNNNNNAGG 1 cut(s) 2910
BstFNI CGCG 1 cut(s) 106
BstH2I RGCGCY 1 cut(s) 1104
BstHHI GCGC 1 cut(s) 1103
BstKTI GATC 5 cut(s) 159, 243, 931, 940, 2891
BstMAI GTCTC 5 cut(s) 183, 319, 423, 1020, 2149
BstMBI GATC 5 cut(s) 156, 240, 928, 937, 2888
BstMCI CGRYCG 1 cut(s) 243
BstNI CCWGG 6 cut(s) 1539, 1927, 2600, 2940, 3150, 3318
BstNSI RCATGY 3 cut(s) 623, 1656, 1684
BstPAI GACNNNNGTC 2 cut(s) 2686, 3279
BstSCI CCNGG 7 cut(s) 1537, 1731, 1925, 2598, 2938, 3148, 3316
BstSFI CTRYAG 4 cut(s) 1715, 2199, 2985, 3190
BstUI CGCG 1 cut(s) 106
BstV1I GCAGC 6 cut(s) 1857, 2340, 2600, 2843, 3176, 3204
BstV2I GAAGAC 2 cut(s) 2720, 3122
BstX2I RGATCY 1 cut(s) 2888
BstXI CCANNNNNNTGG 2 cut(s) 2012, 2151
BstYI RGATCY 1 cut(s) 2888
Bsu36I CCTNAGG 2 cut(s) 1812, 3310
BsuI GTATCC 1 cut(s) 3253
BsuRI GGCC 8 cut(s) 5, 17, 1731, 2238, 2411, 3171, 3207, 3315
BtgI CCRYGG 1 cut(s) 1030
BtrI CACGTC 1 cut(s) 76
BtsIMutI CAGTG 5 cut(s) 2335, 2529, 2575, 2773, 3010
BveI ACCTGC 2 cut(s) 1542, 3093
Cac8I GCNNGC 1 cut(s) 1654
CaiI CAGNNNCTG 2 cut(s) 1921, 2574
CfoI GCGC 1 cut(s) 1103
Cfr13I GGNCC 9 cut(s) 16, 1730, 1929, 2307, 2743, 2981, 2998, 3108, 3314
Csp6I GTAC 9 cut(s) 60, 371, 548, 1286, 1514, 1583, 1983, 2406, 3261
CspCI CAANNNNNGTGG 2 cut(s) 1974, 2009
CviQI GTAC 9 cut(s) 60, 371, 548, 1286, 1514, 1583, 1983, 2406, 3261
DinI GGCGCC 1 cut(s) 1102
DpnI GATC 5 cut(s) 158, 242, 930, 939, 2890
DpnII GATC 5 cut(s) 156, 240, 928, 937, 2888
DraI TTTAAA 2 cut(s) 1461, 3304
DraIII CACNNNGTG 1 cut(s) 2471
EaeI YGGCCR 3 cut(s) 3, 2409, 3169
Eam1104I CTCTTC 6 cut(s) 46, 145, 1086, 2670, 3192, 3326
EarI CTCTTC 6 cut(s) 46, 145, 1086, 2670, 3192, 3326
Ecl136II GAGCTC 1 cut(s) 2020
Eco130I CCWWGG 3 cut(s) 1030, 1179, 1726
Eco24I GRGCYC 1 cut(s) 2022
Eco31I GGTCTC 2 cut(s) 1020, 2149
Eco32I GATATC 1 cut(s) 1778
Eco47I GGWCC 6 cut(s) 1929, 2307, 2743, 2981, 2998, 3108
Eco53kI GAGCTC 1 cut(s) 2020
Eco57I CTGAAG 6 cut(s) 128, 723, 1067, 2202, 2567, 2749
Eco81I CCTNAGG 2 cut(s) 1812, 3310
EcoICRI GAGCTC 1 cut(s) 2020
EcoNI CCTNNNNNAGG 1 cut(s) 2910
EcoO109I RGGNCCY 5 cut(s) 1929, 2307, 2981, 3108, 3314
EcoRII CCWGG 6 cut(s) 1537, 1925, 2598, 2938, 3148, 3316
EcoRV GATATC 1 cut(s) 1778
EcoT14I CCWWGG 3 cut(s) 1030, 1179, 1726
EcoT22I ATGCAT 1 cut(s) 559
EcoT38I GRGCYC 1 cut(s) 2022
EgeI GGCGCC 1 cut(s) 1102
EheI GGCGCC 1 cut(s) 1102
ErhI CCWWGG 3 cut(s) 1030, 1179, 1726
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FaqI GGGAC 6 cut(s) 400, 737, 1515, 2293, 2994, 3121
FauI CCCGC 2 cut(s) 177, 3014
FblI GTMKAC 4 cut(s) 210, 790, 2266, 3067
Fnu4HI GCNGC 8 cut(s) 6, 1846, 2354, 2614, 2857, 3172, 3190, 3193
FriOI GRGCYC 1 cut(s) 2022
Fsp4HI GCNGC 8 cut(s) 6, 1846, 2354, 2614, 2857, 3172, 3190, 3193
FspBI CTAG 7 cut(s) 587, 842, 944, 1005, 1167, 1337, 1391
GlaI GCGC 1 cut(s) 1102
GluI GCNGC 8 cut(s) 6, 1846, 2354, 2614, 2857, 3172, 3190, 3193
GsuI CTGGAG 1 cut(s) 1107
HaeII RGCGCY 1 cut(s) 1104
HaeIII GGCC 8 cut(s) 5, 17, 1731, 2238, 2411, 3171, 3207, 3315
HapII CCGG 5 cut(s) 489, 1733, 2075, 2741, 3246
HhaI GCGC 1 cut(s) 1103
Hin1I GRCGYC 2 cut(s) 1101, 3070
Hin6I GCGC 1 cut(s) 1101
HinP1I GCGC 1 cut(s) 1101
HincII GTYRAC 4 cut(s) 1159, 1531, 2845, 3068
HindII GTYRAC 4 cut(s) 1159, 1531, 2845, 3068
HindIII AAGCTT 1 cut(s) 2693
HpaI GTTAAC 1 cut(s) 1159
HpaII CCGG 5 cut(s) 489, 1733, 2075, 2741, 3246
HphI GGTGA 5 cut(s) 736, 2027, 3065, 3260, 3278
Hpy166II GTNNAC 7 cut(s) 211, 791, 1159, 1531, 2267, 2845, 3068
Hpy8I GTNNAC 7 cut(s) 211, 791, 1159, 1531, 2267, 2845, 3068
Hpy99I CGWCG 3 cut(s) 77, 3069, 3072
HpyCH4III ACNGT 4 cut(s) 887, 1945, 2576, 3384
HpyCH4IV ACGT 3 cut(s) 75, 1902, 3070
HpySE526I ACGT 3 cut(s) 75, 1902, 3070
Hsp92I GRCGYC 2 cut(s) 1101, 3070
HspAI GCGC 1 cut(s) 1101
KasI GGCGCC 1 cut(s) 1100
KflI GGGWCCC 1 cut(s) 3108
Kpn2I TCCGGA 2 cut(s) 488, 2074
KspAI GTTAAC 1 cut(s) 1159
Kzo9I GATC 5 cut(s) 156, 240, 928, 937, 2888
LmnI GCTCC 6 cut(s) 358, 866, 984, 1770, 2389, 2837
Lsp1109I GCAGC 6 cut(s) 1857, 2340, 2600, 2843, 3176, 3204
LweI GCATC 5 cut(s) 451, 2324, 2386, 2475, 2685
MaeI CTAG 7 cut(s) 587, 842, 944, 1005, 1167, 1337, 1391
MaeII ACGT 3 cut(s) 75, 1902, 3070
MaeIII GTNAC 7 cut(s) 1222, 1945, 2570, 2590, 2659, 3071, 3284
MalI GATC 5 cut(s) 158, 242, 930, 939, 2890
MbiI CCGCTC 1 cut(s) 1773
MboI GATC 5 cut(s) 156, 240, 928, 937, 2888
MflI RGATCY 1 cut(s) 2888
MhlI GDGCHC 4 cut(s) 414, 627, 2022, 2422
Mly113I GGCGCC 1 cut(s) 1101
MlyI GAGTC 5 cut(s) 999, 1676, 2734, 3224, 3351
MmeI TCCRAC 5 cut(s) 434, 1399, 2154, 3198, 3352
Mph1103I ATGCAT 1 cut(s) 559
MroI TCCGGA 2 cut(s) 488, 2074
MroXI GAANNNNTTC 4 cut(s) 113, 598, 1080, 1484
MslI CAYNNNNRTG 1 cut(s) 2274
MspA1I CMGCKG 3 cut(s) 2686, 3174, 3279
MspCI CTTAAG 1 cut(s) 179
MspI CCGG 5 cut(s) 489, 1733, 2075, 2741, 3246
MspR9I CCNGG 7 cut(s) 1539, 1733, 1927, 2600, 2940, 3150, 3318
Mva1269I GAATGC 2 cut(s) 559, 1173
MvaI CCWGG 6 cut(s) 1539, 1927, 2600, 2940, 3150, 3318
MvnI CGCG 1 cut(s) 106
NarI GGCGCC 1 cut(s) 1101
NciI CCSGG 1 cut(s) 1733
NcoI CCATGG 1 cut(s) 1030
NdeII GATC 5 cut(s) 156, 240, 928, 937, 2888
NmuCI GTSAC 4 cut(s) 1222, 2570, 3071, 3284
NsiI ATGCAT 1 cut(s) 559
NspI RCATGY 3 cut(s) 623, 1656, 1684
PaeI GCATGC 1 cut(s) 1656
PciI ACATGT 1 cut(s) 619
PctI GAATGC 2 cut(s) 559, 1173
PdmI GAANNNNTTC 4 cut(s) 113, 598, 1080, 1484
PflFI GACNNNGTC 1 cut(s) 2503
PfoI TCCNGGA 1 cut(s) 2598
PkrI GCNGC 8 cut(s) 7, 1847, 2355, 2615, 2858, 3173, 3191, 3194
Ple19I CGATCG 1 cut(s) 243
PleI GAGTC 5 cut(s) 998, 1675, 2733, 3223, 3350
PluTI GGCGCC 1 cut(s) 1104
PpsI GAGTC 5 cut(s) 998, 1675, 2733, 3223, 3350
PpuMI RGGWCCY 4 cut(s) 1929, 2307, 2981, 3108
PscI ACATGT 1 cut(s) 619
PshAI GACNNNNGTC 2 cut(s) 2686, 3279
PshBI ATTAAT 2 cut(s) 1967, 2826
Psp124BI GAGCTC 1 cut(s) 2022
Psp1406I AACGTT 1 cut(s) 1902
Psp5II RGGWCCY 4 cut(s) 1929, 2307, 2981, 3108
Psp6I CCWGG 6 cut(s) 1537, 1925, 2598, 2938, 3148, 3316
PspGI CCWGG 6 cut(s) 1537, 1925, 2598, 2938, 3148, 3316
PspPI GGNCC 9 cut(s) 16, 1730, 1929, 2307, 2743, 2981, 2998, 3108, 3314
PspPPI RGGWCCY 4 cut(s) 1929, 2307, 2981, 3108
PstI CTGCAG 2 cut(s) 1719, 3194
PstNI CAGNNNCTG 2 cut(s) 1921, 2574
PsuI RGATCY 1 cut(s) 2888
PsyI GACNNNGTC 1 cut(s) 2503
PvuI CGATCG 1 cut(s) 243
PvuII CAGCTG 2 cut(s) 2686, 3279
RsaI GTAC 9 cut(s) 61, 372, 549, 1287, 1515, 1584, 1984, 2407, 3262
RsaNI GTAC 9 cut(s) 60, 371, 548, 1286, 1514, 1583, 1983, 2406, 3261
RseI CAYNNNNRTG 1 cut(s) 2274
SacI GAGCTC 1 cut(s) 2022
SalI GTCGAC 1 cut(s) 3066
SatI GCNGC 8 cut(s) 6, 1846, 2354, 2614, 2857, 3172, 3190, 3193
Sau3AI GATC 5 cut(s) 156, 240, 928, 937, 2888
Sau96I GGNCC 9 cut(s) 16, 1730, 1929, 2307, 2743, 2981, 2998, 3108, 3314
SchI GAGTC 5 cut(s) 999, 1676, 2734, 3224, 3351
ScrFI CCNGG 7 cut(s) 1539, 1733, 1927, 2600, 2940, 3150, 3318
SduI GDGCHC 4 cut(s) 414, 627, 2022, 2422
SfaNI GCATC 5 cut(s) 451, 2324, 2386, 2475, 2685
SfcI CTRYAG 4 cut(s) 1715, 2199, 2985, 3190
SfoI GGCGCC 1 cut(s) 1102
SgrDI CGTCGACG 1 cut(s) 3066
SinI GGWCC 6 cut(s) 1929, 2307, 2743, 2981, 2998, 3108
SmiI ATTTAAAT 1 cut(s) 3304
SmiMI CAYNNNNRTG 1 cut(s) 2274
SmlI CTYRAG 2 cut(s) 179, 3290
SmoI CTYRAG 2 cut(s) 179, 3290
SphI GCATGC 1 cut(s) 1656
SsiI CCGC 7 cut(s) 6, 106, 184, 1773, 3007, 3120, 3172
SspDI GGCGCC 1 cut(s) 1100
SspI AATATT 4 cut(s) 760, 2131, 2293, 3254
SspMI CTAG 7 cut(s) 587, 842, 944, 1005, 1167, 1337, 1391
SstI GAGCTC 1 cut(s) 2022
StyD4I CCNGG 7 cut(s) 1537, 1731, 1925, 2598, 2938, 3148, 3316
StyI CCWWGG 3 cut(s) 1030, 1179, 1726
SwaI ATTTAAAT 1 cut(s) 3304
TaaI ACNGT 4 cut(s) 887, 1945, 2576, 3384
TaiI ACGT 3 cut(s) 78, 1905, 3073
TaqI TCGA 4 cut(s) 239, 296, 306, 3067
TatI WGTACW 3 cut(s) 1285, 1582, 1982
TauI GCSGC 2 cut(s) 8, 3174
TscAI CASTG 5 cut(s) 2335, 2536, 2575, 2773, 3017
TseFI GTSAC 4 cut(s) 1222, 2570, 3071, 3284
TseI GCWGC 6 cut(s) 1845, 2353, 2613, 2856, 3189, 3192
Tsp45I GTSAC 4 cut(s) 1222, 2570, 3071, 3284
TspGWI ACGGA 2 cut(s) 1320, 3335
TspRI CASTG 5 cut(s) 2335, 2536, 2575, 2773, 3017
Tth111I GACNNNGTC 1 cut(s) 2503
Vha464I CTTAAG 1 cut(s) 179
VpaK11BI GGWCC 6 cut(s) 1929, 2307, 2743, 2981, 2998, 3108
VspI ATTAAT 2 cut(s) 1967, 2826
XagI CCTNNNNNAGG 1 cut(s) 2910
XbaI TCTAGA 1 cut(s) 1336
XceI RCATGY 3 cut(s) 623, 1656, 1684
XcmI CCANNNNNNNNNTGG 4 cut(s) 971, 1847, 2009, 2440
XmiI GTMKAC 4 cut(s) 210, 790, 2266, 3067
XmnI GAANNNNTTC 4 cut(s) 113, 598, 1080, 1484
XspI CTAG 7 cut(s) 587, 842, 944, 1005, 1167, 1337, 1391
ZraI GACGTC 1 cut(s) 3071
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.