Rorug01G0035700

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000105
Physical Location & Seq
Reverse (-)
5995768 .. 6006135
10368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0035700.1

Sequence Viewer

Length: 4638 bp
ATGACGAAGGTCTACGGAACAGGCGCGTACGATTTCAAGCGCCACCATGTTGCCGAGTACCCGGTGGAGCTGAACCACCAGCTCGGAGAGAAGCCCGTGGTGGAGGCTAAACCCGGTGCGGCGCTGCCGAGCTCGATAACGCTGTCGGAGATTCAGAGGGACCAGCTCACTATGATTGCCGCTGCCAATTGGTCCAAAGCCGGCGGGCAGGTGAAGAAGGCGTTCGACGCGGAGCTGGTGAAGAAGATTTACGAGACGGAGTTGTGTGTGAAGGAGGGGCAGAGGAAGACGGTGCCATTGCAGAGAGTGATGATTTTGGAGGTCAGCCAGTACTTGGAGAACTATCTCTTTCCCAATTTCGATCCCGAAACGGCGACGTTCGAGCACGTTATGTCCATGGTTCTCATGGTCAATGAGAAGTTTCGTGAGAATGTGGCAGCTTGGGTATGCTTTTATGACCGGAAAGATGCCTTTAAGGGATTCCTAGGAAGGGTTCTTCAGCTTAAATCGGAAAGAGAATTAAGTATAGCAGAGAAGACAAATTATCTAGTATTCATGATAAATGCCTTTCAGAGTTTGGAAGATGAGATAGTCAGCGAGACTGTCCTTAGGTTGGCAAGTTTCCAATCCTGGCACAGTTTGTCGTATGGTCGTTTTCAGATGGAGCTAGGTCTAAATTCAGATTTGATCAAGAAATGGAGAAGGATGATAAAGAGGGAAGCTGCAAAACATGGAGAGTCTTTTAACCCCTCAACTACACTTGAAGTCCAGTTTTTGAGAAACCTCATCGAAGAGTTCCTGGAGATACTTGATTCCAAAGTCTTACTGCCAAATCATGGTGTCAATGGAGAGGATCAACTTCTTGATGCCAATGGAATGGAACACATTGATGATGCTTGTGTTTTGTACTGTGAGAGGTTTATAGAATTTCTTATTGACCTCTTGAGCCAACTACCAACAAGGAGGTACTTGAGGCCACTTGTTGCTGATGTTGCTGTTGTTCCGAAATGCCACTTGAGTGCATTGTATAGACATGAAAAGGGGAAGCTCTTTACCCAACTTGTTGACTTGCTGCAGTTTTATGAAGGATTTGAGATTAATGATAATGTCGGGAAACAATTGACGGATGATGAAGTGCTCCAATCTCATTATGATCGAGTACAATCTTTCCAGCTGCTTGCTTTTAAAAAGATTCCTAAGTTGCGAGAGCTTGCATTGGCCAACATTGGTTCAATTGACAACCGAAATGATCTCACTAAGAGATTGTCTGTTCTTTCTCCTGAAGAATTGAAGGATTTGGTTTGTTCAAAGTTGAAACTGATTTCTAAGAATGATCCTTGGTCATCCAGGGTTGATTTCCTTACTGAAGTGATGGTTTCCTTTTTCAAGAGGCAACAGTCTCAAAAAGAAAAAATAAATGCTCTTCCACTGTATCCAAATGAGCTTATCATGTGGGATGAAAGCCTTGTGCCAAGCATTAATTACTCTGGAGAAGGTTGTCTGGCTCTTCCAAAACTTAATTTACAATTTTTGACGCTGCATGATTATCTCCTCAGAAATTTTGATCTCTTCCGTCTCGAATCAACATATGAGATTCGTGAGGATATTCAGGAGGCTGTGCCACATCTTAATGCTTGCATTAATAATGAGGGAGAAACTGCTTTCCGTGGTTGGTCAAGGATGGCTGTGCCGATTAAAGAATTCAAGATCAGTGAGGTAAAACAGCCAAATATTGGAGAAGTTAAGCCAGCTGCTGTGACAGCAGAAATCACTTTTAGTATATCCAGTTATAAAGCACAGGTACGATCAGAATGGAATGCTCTTAAAGAGCATGATGTTCTATTTTTACTTTCCATACGCCCTTCCTTTGAGCCTCTAAGTGCAGAAGAGGGTGCCAAGGCTAGTGTGCCTCAGAGGCTTGGTCTTCAATATGTGCGGGGTTGTGAAATCATTGAGGTTCGTGATGAGGAGGGAACTCTAATGAATGATTTCAGTGGAAGAATTAAACGGGATGAGTGGAAGCCGCCAAAAGGAGAGTTGAGAACTTTGACTGTTGCTTTGGATACCGCTCAGTACTACATGGATGTTAGCAACACTGCTGCCAAGGGTGCAGAAGATGTTTATGGGACATTCAATATTTTGATGAGGAGAAAGCCCAAGGAAAATAATTTCAAAGCAATTTTAGAATCCATTAGAGATCTTATGAATGAATATTGTATTGTTCCTGACTGGTTGCACAAAACATTTCTGGGGTATGGTAATCCTTCTGCCGCACAATGGACTAATATGCCAGATCTCTTAGAAACCGTAGATTTCAAAGATACTTTCCTTGATGCAGACCATCTGAAGGAATGTTTTCCAGATGATCAGGTTTTCTTTGTTAGTCCAGATGGTACGGAAAATATGGATCCAGGGCCTCCTTTTCGAATAAGGCTTCCCAAGAAGATCAAAAGCAGCACTAATGCTCTTCCAGGCAATAAGAAAGCAAAGATTAATTCTATGAGTGATGTCCCCATTGAGGACTCTGATAGTGAGAAAGAAAAATTTGTTGTCGAGGCATACACCCCTCCTGACCCAGGGCCTTATCCGCAAGACCAGCCAAGGCAGAACTCAGTTAGATTTACACCCACTCAGGTTGGAGCAATCCTCTCAGGTATTCAGCCAGGACTAACAATGGTTGTGGGTCCACCTGGTACGGGAAAGACAGATACAGCCGTGCAAATTTTGAATGTTCTGTATCATAATTGTCCATCTCAGAGAACATTGATAATTACTCACTCAAACCAGGCTCTAAATGACCTTTTTGAAAAGATAATGCAGAGGGATGTACCTGCACGCTATCTTCTTCGACTTGGGCAAGGAGAACAAGAGCTAGCAACTGATCTTGACTTCAGTCGGCAAGGCCGTGTCAATGCCATGCTTGTGAGGCGGTTGGAGTTGCTTAGTGAAGTGGAGAGGCTGGCAAGATCTCTCCAATTGCCTGAGGATGTTGGTTATACATGTGAAACTGCTGGATACTTTTGGTTGCTTCATGTATACTCGCGCTGGGAGCAATTCCTGGCTGCTTGTAACCAAAATAAAAATAAACAATCATTTGTTAAAGATCGATTTCCCTTCAAGGAATTCTTCTCCAACGCACCAAAGCCAGTTTTTACTGGTGAGTCATTTGAGAAAGATATGCGGGCAGCTAAGGGTTGCTTTCGTCATCTAAAGACTGTGTTTCAAGAGCTTGAAGAGTGTAGGGCTTTTGAGTTACTGAAGTCTACAGCTGATCGAGCAAACTACTTGATGACGAAGCAGGCAAAGATAGTTGCCATGACATGCACTCACGCAGCTCTGAAGAGGAAGGATTTTCTCCAGTTAGGTTTCAAGTATGATAACTTGCTGATGGAAGAAAGTGCTCAAATTTTGGAGATCGAAACTTTCATTCCAATGTTGCTCCAGAGGCAGGAAGATGGTTATGCACGGCTTAAACGTTGTATCCTGATTGGTGATCATCACCAGTTGCCTCCTGTTGTCAAGAATATGGCCTTTCAAAAGTATAGCCACATGGACCAGAGTCTCTTTACAAGGTTTGTCCGTTTGGGCATTCCTTATATTGAACTTAATGCCCAGGGTAGGGCCAGGCCAAGTATAGCCAAACTTTACAACTGGAGGTATAGAGAACTAGGAGACCTTCCTAATGTAAAGGAAGATGCCGTTTACAATAGAGCAAATTCTGGATTCTCCTTTGAATATCAATTGGTAGATGTACCAGATTACCATGATAGAGGTGAGTCTGCCCCATCACCTTGGTTCTACCAAAATGAGGGAGAAGCTGAATATATCGTCAGTGTGTATATCTACATGCGTCTACTTGGGTACCCTGCAAACAAGATATCAATATTGACCACTTACAATGGCCAGAAGCTTTTAATCCGTGATGTTATCAACAGACGATGTGCTCCGTATGACTTCATAGGCCCTCCCAGCAAGGTTACAACAGTTGACAAATTTCAGGGTCAGCAAAATGATTTTATATTGCTCTCTCTTGTGCGTACTCGCTTTGTGGGTCACCTTCGTGATGTTAGGAGATTGATTGTTGCCATGTCTCGTGCCCGATTGGGTCTGTATGTGTTTTGCCGCCGTTCTTTGTTTGAGCAATGCTATGAACTTCAGCCTACCTTCCAACTTTTACTTCAGAGACCTGATCGCCTTGCTCTGAATTTTAATGAGACTACACCACACACAGAACGCCATGTTGAAGAAACGGGGCCTGTCCATCTTGTAAGCAGTGTTGATGAGATGATCAGTATCTACCAGCAGCTTTATGCGGTGAAGTTTCATCAGTATGTTGCTCCTTCAAGTCAATTAGTTCCATCCATGGATGCATTGGGAGAGCAAACAAGTCAGCAAACTGATATGTCTGGGCAAATTCCTATGGATGCAGATATCCCTGTATCTGCTGATGGGGCGCCAGACTTTACACTGCATGGAAGCAATTCAGAACTGGGTGAAAAATTGGATGTCGCAAATGGCCAAAAAGTGGATTCGTCAATTGAGAATCATTCAAATGGGGTGACGGAAATGGAAACTAGTCTGGATGATGCGCATGTGGCACCTGAAAGCAATTCGGATCAAGTGAAAATGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1545

Amino Acids

176.72

Weight (kDa)

5.7

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aquarius_N_1st PF16399 56 - 466 1.1e-134 Intron-binding protein aquarius N-terminal
Aquarius_N_2nd PF21143 548 - 717 7.4e-57 Intron-binding protein aquarius, beta-barrel
Aquarius_N_3rd PF21144 767 - 866 3e-21 Intron-binding protein aquarius insert domain
AAA_11 PF13086 879 - 1175 1.2e-28 AAA domain
AAA_12 PF13087 1184 - 1376 2.1e-27 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1791
AarI CACCTGC 1 cut(s) 199
Acc16I TGCGCA 1 cut(s) 4590
Acc36I ACCTGC 2 cut(s) 199, 2839
Acc65I GGTACC 1 cut(s) 3861
AccB1I GGYRCC 5 cut(s) 292, 1892, 3861, 4453, 4597
AccB7I CCANNNNNTGG 4 cut(s) 334, 836, 1733, 4525
AccBSI CCGCTC 1 cut(s) 2069
AccI GTMKAC 4 cut(s) 12, 3036, 3263, 3853
AccII CGCG 3 cut(s) 26, 230, 3043
AclI AACGTT 1 cut(s) 3475
AclWI GGATC 6 cut(s) 356, 861, 1328, 2402, 2415, 4623
AcoI YGGCCR 3 cut(s) 1218, 3901, 4516
AcuI CTGAAG 9 cut(s) 482, 1302, 1386, 2366, 2875, 3278, 3359, 4139, 4163
AcyI GRCGYC 1 cut(s) 4454
AflIII ACRYGT 1 cut(s) 2999
AhlI ACTAGT 1 cut(s) 4574
AjuI GAANNNNNNNTTGG 2 cut(s) 4161, 4193
AleI CACNNNNGTG 2 cut(s) 1017, 4059
Alw21I GWGCWC 5 cut(s) 134, 387, 1140, 3403, 3946
Alw26I GTCTC 9 cut(s) 248, 593, 1404, 1580, 3566, 3666, 4095, 4177, 4208
AlwI GGATC 6 cut(s) 356, 861, 1328, 2402, 2415, 4623
AlwNI CAGNNNCTG 1 cut(s) 1754
ArsI GACNNNNNNTTYG 2 cut(s) 2041, 2073
AseI ATTAAT 4 cut(s) 1098, 1479, 1641, 2493
Asp700I GAANNNNTTC 4 cut(s) 221, 1988, 2355, 4480
Asp718I GGTACC 1 cut(s) 3861
AspA2I CCTAGG 1 cut(s) 484
AspLEI GCGC 6 cut(s) 26, 42, 124, 3045, 4456, 4591
AspS9I GGNCC 9 cut(s) 160, 192, 2414, 2579, 2684, 3553, 3621, 3962, 4253
AsuC2I CCSGG 2 cut(s) 62, 114
AsuII TTCGAA 1 cut(s) 2425
AsuNHI GCTAGC 1 cut(s) 2872
AvaII GGWCC 4 cut(s) 160, 192, 2684, 3553
AvrII CCTAGG 1 cut(s) 484
AxyI CCTNAGG 2 cut(s) 608, 2982
BaeGI GKGCMC 1 cut(s) 4099
BaeI ACNNNNGTAYC 4 cut(s) 2700, 2733, 3845, 3878
BalI TGGCCA 3 cut(s) 1220, 3903, 4518
BamHI GGATCC 1 cut(s) 2407
BanI GGYRCC 5 cut(s) 292, 1892, 3861, 4453, 4597
BanII GRGCYC 1 cut(s) 134
BarI GAAGNNNNNNTAC 2 cut(s) 233, 265
BauI CACGAG 1 cut(s) 4092
BbsI GAAGAC 3 cut(s) 293, 542, 1916
Bbv12I GWGCWC 5 cut(s) 134, 387, 1140, 3403, 3946
BceAI ACGGC 6 cut(s) 387, 2699, 2889, 3482, 3683, 4110
BciVI GTATCC 4 cut(s) 1443, 2056, 3008, 3491
BclI TGATCA 4 cut(s) 687, 2365, 3493, 4287
BcnI CCSGG 2 cut(s) 62, 114
BcoDI GTCTC 9 cut(s) 248, 593, 1404, 1580, 3566, 3666, 4095, 4177, 4208
BcuI ACTAGT 1 cut(s) 4574
BfaI CTAG 7 cut(s) 485, 548, 668, 1902, 2873, 3668, 4575
BfmI CTRYAG 2 cut(s) 1073, 3264
BfoI RGCGCY 3 cut(s) 43, 125, 4457
BfuAI ACCTGC 2 cut(s) 199, 2839
BfuI GTATCC 4 cut(s) 1443, 2056, 3008, 3491
BglI GCCNNNNNGGC 1 cut(s) 1915
BglII AGATCT 3 cut(s) 2197, 2293, 2966
BlnI CCTAGG 1 cut(s) 484
BmcAI AGTACT 2 cut(s) 332, 2075
Bme18I GGWCC 4 cut(s) 160, 192, 2684, 3553
BmgT120I GGNCC 9 cut(s) 160, 192, 2414, 2579, 2684, 3553, 3621, 3962, 4253
BmiI GGNNCC 9 cut(s) 161, 294, 1894, 2409, 2685, 3863, 4254, 4455, 4599
BmrI ACTGGG 1 cut(s) 4499
BmsI GCATC 8 cut(s) 457, 856, 883, 2323, 3685, 4357, 4414, 4576
BmtI GCTAGC 1 cut(s) 2876
BmuI ACTGGG 1 cut(s) 4499
BoxI GACNNNNGTC 3 cut(s) 8, 2892, 3558
BpiI GAAGAC 3 cut(s) 293, 542, 1916
BplI GAGNNNNNCTC 2 cut(s) 2631, 2663
BpmI CTGGAG 5 cut(s) 821, 1509, 3341, 3425, 3673
Bpu10I CCTNAGC 1 cut(s) 3189
Bpu14I TTCGAA 1 cut(s) 2425
BpuEI CTTGAG 3 cut(s) 964, 991, 1036
BpuMI CCSGG 2 cut(s) 62, 114
Bsa29I ATCGAT 1 cut(s) 3106
BsaBI GATNNNNATC 1 cut(s) 4292
BsaHI GRCGYC 1 cut(s) 4454
BsaI GGTCTC 2 cut(s) 3666, 4177
BsaWI WCCGGW 1 cut(s) 459
Bse118I RCCGGY 1 cut(s) 200
Bse21I CCTNAGG 2 cut(s) 608, 2982
Bse3DI GCAATG 2 cut(s) 296, 4148
Bse8I GATNNNNATC 1 cut(s) 4292
BseCI ATCGAT 1 cut(s) 3106
BseJI GATNNNNATC 1 cut(s) 4292
BseMI GCAATG 2 cut(s) 296, 4148
BseMII CTCAG 8 cut(s) 1567, 1925, 2084, 2625, 2645, 2664, 2768, 2973
BseRI GAGGAG 3 cut(s) 1541, 1982, 2161
BseSI GKGCMC 1 cut(s) 4099
BseYI CCCAGC 2 cut(s) 3045, 3968
BsgI GTGCAG 3 cut(s) 1902, 2130, 2817
Bsh1236I CGCG 3 cut(s) 26, 230, 3043
BshNI GGYRCC 5 cut(s) 292, 1892, 3861, 4453, 4597
BshVI ATCGAT 1 cut(s) 3106
BsiHKAI GWGCWC 5 cut(s) 134, 387, 1140, 3403, 3946
BsiSI CCGG 4 cut(s) 62, 114, 201, 460
BsiWI CGTACG 1 cut(s) 27
BslFI GGGAC 3 cut(s) 173, 2140, 2495
BsmAI GTCTC 9 cut(s) 248, 593, 1404, 1580, 3566, 3666, 4095, 4177, 4208
BsmBI CGTCTC 2 cut(s) 248, 1580
BsmFI GGGAC 3 cut(s) 173, 2140, 2495
BsmI GAATGC 2 cut(s) 1822, 3588
Bso31I GGTCTC 2 cut(s) 3666, 4177
Bsp119I TTCGAA 1 cut(s) 2425
Bsp1286I GDGCHC 6 cut(s) 134, 387, 1140, 3403, 3946, 4099
Bsp19I CCATGG 2 cut(s) 396, 4362
BspCNI CTCAG 8 cut(s) 1566, 1924, 2083, 2624, 2644, 2663, 2767, 2974
BspDI ATCGAT 1 cut(s) 3106
BspFNI CGCG 3 cut(s) 26, 230, 3043
BspHI TCATGA 1 cut(s) 555
BspLI GGNNCC 9 cut(s) 161, 294, 1894, 2409, 2685, 3863, 4254, 4455, 4599
BspMAI CTGCAG 1 cut(s) 1077
BspMI ACCTGC 2 cut(s) 199, 2839
BspOI GCTAGC 1 cut(s) 2876
BspPI GGATC 6 cut(s) 356, 861, 1328, 2402, 2415, 4623
BspQI GCTCTTC 3 cut(s) 1428, 1512, 2472
BspT104I TTCGAA 1 cut(s) 2425
BspT107I GGYRCC 5 cut(s) 292, 1892, 3861, 4453, 4597
BspTNI GGTCTC 2 cut(s) 3666, 4177
BsrBI CCGCTC 1 cut(s) 2069
BsrDI GCAATG 2 cut(s) 296, 4148
BsrFI RCCGGY 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 200
BssNAI GTATAC 1 cut(s) 3037
BssNI GRCGYC 1 cut(s) 4454
BssSI CACGAG 1 cut(s) 4092
BssT1I CCWWGG 9 cut(s) 396, 484, 1337, 1896, 2103, 2157, 2600, 3791, 4362
Bst1107I GTATAC 1 cut(s) 3037
Bst2BI CACGAG 1 cut(s) 4092
Bst6I CTCTTC 9 cut(s) 786, 1428, 1512, 1574, 1881, 2472, 3228, 3335, 4626
BstACI GRCGYC 1 cut(s) 4454
BstBI TTCGAA 1 cut(s) 2425
BstDSI CCRYGG 4 cut(s) 96, 396, 1666, 4362
BstEII GGTNACC 1 cut(s) 4052
BstENI CCTNNNNNAGG 1 cut(s) 2574
BstFNI CGCG 3 cut(s) 26, 230, 3043
BstH2I RGCGCY 3 cut(s) 43, 125, 4457
BstHHI GCGC 6 cut(s) 26, 42, 124, 3045, 4456, 4591
BstMAI GTCTC 9 cut(s) 248, 593, 1404, 1580, 3566, 3666, 4095, 4177, 4208
BstNSI RCATGY 4 cut(s) 3003, 3324, 3850, 4595
BstPAI GACNNNNGTC 3 cut(s) 8, 2892, 3558
BstPI GGTNACC 1 cut(s) 4052
BstSFI CTRYAG 2 cut(s) 1073, 3264
BstSLI GKGCMC 1 cut(s) 4099
BstUI CGCG 3 cut(s) 26, 230, 3043
BstV2I GAAGAC 3 cut(s) 293, 542, 1916
BstX2I RGATCY 4 cut(s) 2197, 2293, 2407, 2966
BstXI CCANNNNNNTGG 2 cut(s) 877, 3792
BstYI RGATCY 4 cut(s) 2197, 2293, 2407, 2966
BstZ17I GTATAC 1 cut(s) 3037
Bsu15I ATCGAT 1 cut(s) 3106
Bsu36I CCTNAGG 2 cut(s) 608, 2982
BsuI GTATCC 4 cut(s) 1443, 2056, 3008, 3491
BsuTUI ATCGAT 1 cut(s) 3106
BtgI CCRYGG 4 cut(s) 96, 396, 1666, 4362
BtsI GCAGTG 3 cut(s) 2094, 4279, 4466
BtsIMutI CAGTG 7 cut(s) 1427, 1717, 1999, 2094, 3838, 4279, 4466
BveI ACCTGC 2 cut(s) 199, 2839
CaiI CAGNNNCTG 1 cut(s) 1754
CciI TCATGA 1 cut(s) 555
CfoI GCGC 6 cut(s) 26, 42, 124, 3045, 4456, 4591
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 9 cut(s) 160, 192, 2414, 2579, 2684, 3553, 3621, 3962, 4253
ClaI ATCGAT 1 cut(s) 3106
CseI GACGC 3 cut(s) 236, 1543, 3839
CsiI ACCWGGT 1 cut(s) 2689
CspCI CAANNNNNGTGG 4 cut(s) 2617, 2652, 2661, 2696
DinI GGCGCC 1 cut(s) 4455
DraI TTTAAA 1 cut(s) 1186
EaeI YGGCCR 3 cut(s) 1218, 3901, 4516
Eam1104I CTCTTC 9 cut(s) 786, 1428, 1512, 1574, 1881, 2472, 3228, 3335, 4626
EarI CTCTTC 9 cut(s) 786, 1428, 1512, 1574, 1881, 2472, 3228, 3335, 4626
Ecl136II GAGCTC 1 cut(s) 132
Eco130I CCWWGG 9 cut(s) 396, 484, 1337, 1896, 2103, 2157, 2600, 3791, 4362
Eco24I GRGCYC 1 cut(s) 134
Eco31I GGTCTC 2 cut(s) 3666, 4177
Eco32I GATATC 2 cut(s) 3879, 4432
Eco47I GGWCC 4 cut(s) 160, 192, 2684, 3553
Eco53kI GAGCTC 1 cut(s) 132
Eco57I CTGAAG 9 cut(s) 482, 1302, 1386, 2366, 2875, 3278, 3359, 4139, 4163
Eco81I CCTNAGG 2 cut(s) 608, 2982
Eco91I GGTNACC 1 cut(s) 4052
EcoICRI GAGCTC 1 cut(s) 132
EcoNI CCTNNNNNAGG 1 cut(s) 2574
EcoO109I RGGNCCY 4 cut(s) 2414, 2579, 3962, 4253
EcoO65I GGTNACC 1 cut(s) 4052
EcoRI GAATTC 2 cut(s) 1700, 3122
EcoRV GATATC 2 cut(s) 3879, 4432
EcoT14I CCWWGG 9 cut(s) 396, 484, 1337, 1896, 2103, 2157, 2600, 3791, 4362
EcoT22I ATGCAT 1 cut(s) 4372
EcoT38I GRGCYC 1 cut(s) 134
EgeI GGCGCC 1 cut(s) 4455
EheI GGCGCC 1 cut(s) 4455
ErhI CCWWGG 9 cut(s) 396, 484, 1337, 1896, 2103, 2157, 2600, 3791, 4362
Esp3I CGTCTC 2 cut(s) 248, 1580
FalI AAGNNNNNCTT 4 cut(s) 3110, 3142, 3182, 3214
FaqI GGGAC 3 cut(s) 173, 2140, 2495
FauI CCCGC 3 cut(s) 197, 1929, 3174
FauNDI CATATG 1 cut(s) 1588
FbaI TGATCA 4 cut(s) 687, 2365, 3493, 4287
FblI GTMKAC 4 cut(s) 12, 3036, 3263, 3853
FriOI GRGCYC 1 cut(s) 134
FspAI RTGCGCAY 1 cut(s) 4590
FspBI CTAG 7 cut(s) 485, 548, 668, 1902, 2873, 3668, 4575
FspI TGCGCA 1 cut(s) 4590
GlaI GCGC 6 cut(s) 25, 41, 123, 3044, 4455, 4590
GsaI CCCAGC 2 cut(s) 3049, 3972
GsuI CTGGAG 5 cut(s) 821, 1509, 3341, 3425, 3673
HaeII RGCGCY 3 cut(s) 43, 125, 4457
HapII CCGG 4 cut(s) 62, 114, 201, 460
HgaI GACGC 3 cut(s) 236, 1543, 3839
HhaI GCGC 6 cut(s) 26, 42, 124, 3045, 4456, 4591
Hin1I GRCGYC 1 cut(s) 4454
Hin6I GCGC 6 cut(s) 24, 40, 122, 3043, 4454, 4589
HinP1I GCGC 6 cut(s) 24, 40, 122, 3043, 4454, 4589
HincII GTYRAC 2 cut(s) 1066, 3988
HindII GTYRAC 2 cut(s) 1066, 3988
HindIII AAGCTT 1 cut(s) 3908
HpaII CCGG 4 cut(s) 62, 114, 201, 460
Hpy166II GTNNAC 8 cut(s) 13, 1066, 2687, 3037, 3264, 3703, 3854, 3988
Hpy8I GTNNAC 8 cut(s) 13, 1066, 2687, 3037, 3264, 3703, 3854, 3988
Hpy99I CGWCG 2 cut(s) 230, 379
HpyCH4IV ACGT 3 cut(s) 377, 387, 3475
HpySE526I ACGT 3 cut(s) 377, 387, 3475
Hsp92I GRCGYC 1 cut(s) 4454
HspAI GCGC 6 cut(s) 24, 40, 122, 3043, 4454, 4589
KasI GGCGCC 1 cut(s) 4453
KpnI GGTACC 1 cut(s) 3865
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Ksp22I TGATCA 4 cut(s) 687, 2365, 3493, 4287
LguI GCTCTTC 3 cut(s) 1428, 1512, 2472
LmnI GCTCC 9 cut(s) 67, 232, 664, 1143, 2639, 3049, 3444, 3949, 4342
LweI GCATC 8 cut(s) 457, 856, 883, 2323, 3685, 4357, 4414, 4576
MabI ACCWGGT 1 cut(s) 2689
MaeI CTAG 7 cut(s) 485, 548, 668, 1902, 2873, 3668, 4575
MaeII ACGT 3 cut(s) 377, 387, 3475
MaeIII GTNAC 6 cut(s) 1756, 3068, 3252, 3976, 4052, 4558
MbiI CCGCTC 1 cut(s) 2069
MfeI CAATTG 6 cut(s) 187, 1118, 1233, 2975, 3740, 4536
MflI RGATCY 4 cut(s) 2197, 2293, 2407, 2966
MhlI GDGCHC 6 cut(s) 134, 387, 1140, 3403, 3946, 4099
MlsI TGGCCA 3 cut(s) 1220, 3903, 4518
MluNI TGGCCA 3 cut(s) 1220, 3903, 4518
Mly113I GGCGCC 1 cut(s) 4454
MlyI GAGTC 5 cut(s) 746, 2516, 3170, 3568, 3785
MmeI TCCRAC 5 cut(s) 126, 2617, 2913, 3156, 4192
Mox20I TGGCCA 3 cut(s) 1220, 3903, 4518
Mph1103I ATGCAT 1 cut(s) 4372
MroNI GCCGGC 1 cut(s) 200
MroXI GAANNNNTTC 4 cut(s) 221, 1988, 2355, 4480
MscI TGGCCA 3 cut(s) 1220, 3903, 4518
MslI CAYNNNNRTG 8 cut(s) 888, 1017, 1629, 2921, 3431, 4059, 4329, 4551
Msp20I TGGCCA 3 cut(s) 1220, 3903, 4518
MspA1I CMGCKG 4 cut(s) 182, 1174, 1751, 3269
MspI CCGG 4 cut(s) 62, 114, 201, 460
MunI CAATTG 6 cut(s) 187, 1118, 1233, 2975, 3740, 4536
Mva1269I GAATGC 2 cut(s) 1822, 3588
MvnI CGCG 3 cut(s) 26, 230, 3043
NaeI GCCGGC 1 cut(s) 202
NarI GGCGCC 1 cut(s) 4454
NciI CCSGG 2 cut(s) 62, 114
NcoI CCATGG 2 cut(s) 396, 4362
NdeI CATATG 1 cut(s) 1588
NgoMIV GCCGGC 1 cut(s) 200
NheI GCTAGC 1 cut(s) 2872
NlaIV GGNNCC 9 cut(s) 161, 294, 1894, 2409, 2685, 3863, 4254, 4455, 4599
NmeAIII GCCGAG 2 cut(s) 79, 153
NmuCI GTSAC 3 cut(s) 1756, 4052, 4558
NsbI TGCGCA 1 cut(s) 4590
NsiI ATGCAT 1 cut(s) 4372
NspI RCATGY 4 cut(s) 3003, 3324, 3850, 4595
NspV TTCGAA 1 cut(s) 2425
OliI CACNNNNGTG 2 cut(s) 1017, 4059
PagI TCATGA 1 cut(s) 555
PaqCI CACCTGC 1 cut(s) 199
PasI CCCWGGG 2 cut(s) 2576, 3613
PciI ACATGT 1 cut(s) 2999
PciSI GCTCTTC 3 cut(s) 1428, 1512, 2472
PcsI WCGNNNNNNNCGW 2 cut(s) 2902, 3472
PctI GAATGC 2 cut(s) 1822, 3588
PdiI GCCGGC 1 cut(s) 202
PdmI GAANNNNTTC 4 cut(s) 221, 1988, 2355, 4480
Pfl23II CGTACG 1 cut(s) 27
PflMI CCANNNNNTGG 4 cut(s) 334, 836, 1733, 4525
PfoI TCCNGGA 1 cut(s) 798
PleI GAGTC 5 cut(s) 745, 2516, 3169, 3567, 3784
PluTI GGCGCC 1 cut(s) 4457
PpsI GAGTC 5 cut(s) 745, 2516, 3169, 3567, 3784
PscI ACATGT 1 cut(s) 2999
PshAI GACNNNNGTC 3 cut(s) 8, 2892, 3558
PshBI ATTAAT 4 cut(s) 1098, 1479, 1641, 2493
PsiI TTATAA 1 cut(s) 1791
Psp124BI GAGCTC 1 cut(s) 134
Psp1406I AACGTT 1 cut(s) 3475
PspEI GGTNACC 1 cut(s) 4052
PspFI CCCAGC 2 cut(s) 3045, 3968
PspLI CGTACG 1 cut(s) 27
PspN4I GGNNCC 9 cut(s) 161, 294, 1894, 2409, 2685, 3863, 4254, 4455, 4599
PspPI GGNCC 9 cut(s) 160, 192, 2414, 2579, 2684, 3553, 3621, 3962, 4253
PstI CTGCAG 1 cut(s) 1077
PstNI CAGNNNCTG 1 cut(s) 1754
PsuI RGATCY 4 cut(s) 2197, 2293, 2407, 2966
PvuII CAGCTG 3 cut(s) 1174, 1751, 3269
RseI CAYNNNNRTG 8 cut(s) 888, 1017, 1629, 2921, 3431, 4059, 4329, 4551
SacI GAGCTC 1 cut(s) 134
SapI GCTCTTC 3 cut(s) 1428, 1512, 2472
Sau96I GGNCC 9 cut(s) 160, 192, 2414, 2579, 2684, 3553, 3621, 3962, 4253
ScaI AGTACT 2 cut(s) 332, 2075
SchI GAGTC 5 cut(s) 746, 2516, 3170, 3568, 3785
SduI GDGCHC 6 cut(s) 134, 387, 1140, 3403, 3946, 4099
SexAI ACCWGGT 1 cut(s) 2689
SfaNI GCATC 8 cut(s) 457, 856, 883, 2323, 3685, 4357, 4414, 4576
SfcI CTRYAG 2 cut(s) 1073, 3264
SfoI GGCGCC 1 cut(s) 4455
SfuI TTCGAA 1 cut(s) 2425
SinI GGWCC 4 cut(s) 160, 192, 2684, 3553
SmiMI CAYNNNNRTG 8 cut(s) 888, 1017, 1629, 2921, 3431, 4059, 4329, 4551
SmlI CTYRAG 3 cut(s) 943, 970, 1015
SmoI CTYRAG 3 cut(s) 943, 970, 1015
SpeI ACTAGT 1 cut(s) 4574
SspDI GGCGCC 1 cut(s) 4453
SspI AATATT 4 cut(s) 1732, 2137, 2213, 3885
SspMI CTAG 7 cut(s) 485, 548, 668, 1902, 2873, 3668, 4575
SstI GAGCTC 1 cut(s) 134
StyI CCWWGG 9 cut(s) 396, 484, 1337, 1896, 2103, 2157, 2600, 3791, 4362
TaiI ACGT 3 cut(s) 380, 390, 3478
TatI WGTACW 4 cut(s) 330, 906, 1159, 2073
TauI GCSGC 5 cut(s) 122, 182, 2026, 2273, 4125
TscAI CASTG 7 cut(s) 1434, 1717, 1999, 2101, 3838, 4279, 4473
TseFI GTSAC 3 cut(s) 1756, 4052, 4558
Tsp45I GTSAC 3 cut(s) 1756, 4052, 4558
TspRI CASTG 7 cut(s) 1434, 1717, 1999, 2101, 3838, 4279, 4473
Van91I CCANNNNNTGG 4 cut(s) 334, 836, 1733, 4525
VpaK11BI GGWCC 4 cut(s) 160, 192, 2684, 3553
VspI ATTAAT 4 cut(s) 1098, 1479, 1641, 2493
XagI CCTNNNNNAGG 1 cut(s) 2574
XceI RCATGY 4 cut(s) 3003, 3324, 3850, 4595
XcmI CCANNNNNNNNNTGG 2 cut(s) 403, 4369
XmaJI CCTAGG 1 cut(s) 484
XmiI GTMKAC 4 cut(s) 12, 3036, 3263, 3853
XmnI GAANNNNTTC 4 cut(s) 221, 1988, 2355, 4480
XspI CTAG 7 cut(s) 485, 548, 668, 1902, 2873, 3668, 4575
ZrmI AGTACT 2 cut(s) 332, 2075
Zsp2I ATGCAT 1 cut(s) 4372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.