Rw1G004510

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Reverse (-)
8687139 .. 8687831
693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004510.1

Sequence Viewer

Length: 693 bp
ATGGAATCTCTTGAAACATTTATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCTGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCCATTGAGGAACTGCCTATTTCAATTGAACGGCTGACTGGCCTTGTGACATTGAATCTATGCAACTGCAGAAATCTTGTTTGTCTTCCAGGCACCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAATGTGGGGGAGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTTTGCAATAGAAATATCATCTACGCATGATCACAGAAAATATGTGAGATTTTCAATCTTTCATGGATGCAAAGTAGTTTGGCGATCTTTAAATAAGTTCTTGCCTTCAGGATTGGTACAAAAAGAGCCAATGAGTTTCCGCTTGCCTATATATGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTCGGTGAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACGTTGAATCTAAGTGGGAACAATTTTGTTCGTCTTCCTATAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGACTGCAAGAGTTGTCAGACCTTCCATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

230

Amino Acids

25.72

Weight (kDa)

7.98

Isoelectric Point (pI)

29.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_13 PF23286 2 - 87 1.6e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 13 - 83 2.2e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 200
AciI CCGC 1 cut(s) 460
AclI AACGTT 1 cut(s) 79
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 1 cut(s) 411
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 1 cut(s) 473
AgsI TTSAA 9 cut(s) 14, 132, 137, 163, 217, 227, 253, 375, 574
AjnI CCWGG 1 cut(s) 196
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AluBI AGCT 1 cut(s) 631
AluI AGCT 1 cut(s) 631
Alw26I GTCTC 1 cut(s) 98
AoxI GGCC 1 cut(s) 148
ApoI RAATTY 1 cut(s) 542
AsuHPI GGTGA 2 cut(s) 533, 577
BanI GGYRCC 1 cut(s) 200
BbsI GAAGAC 2 cut(s) 185, 593
BccI CCATC 3 cut(s) 212, 280, 692
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 198
BclI TGATCA 1 cut(s) 349
BcoDI GTCTC 1 cut(s) 98
BfmI CTRYAG 2 cut(s) 175, 606
Bme1390I CCNGG 1 cut(s) 198
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 1 cut(s) 198
BmsI GCATC 2 cut(s) 236, 377
BpiI GAAGAC 2 cut(s) 185, 593
BpuEI CTTGAG 1 cut(s) 653
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse1I ACTGG 1 cut(s) 151
BseBI CCWGG 1 cut(s) 198
BseGI GGATG 2 cut(s) 251, 392
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMII CTCAG 2 cut(s) 48, 494
BseNI ACTGG 1 cut(s) 151
BshFI GGCC 1 cut(s) 150
BshNI GGYRCC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 473
BsmAI GTCTC 1 cut(s) 98
BsnI GGCC 1 cut(s) 150
Bsp143I GATC 2 cut(s) 349, 404
BspACI CCGC 1 cut(s) 460
BspANI GGCC 1 cut(s) 150
BspCNI CTCAG 2 cut(s) 49, 495
BspLI GGNNCC 1 cut(s) 202
BspMAI CTGCAG 1 cut(s) 179
BspT107I GGYRCC 1 cut(s) 200
BsrI ACTGG 1 cut(s) 151
BssMI GATC 2 cut(s) 349, 404
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 464
BstDEI CTNAG 4 cut(s) 57, 503, 578, 627
BstF5I GGATG 2 cut(s) 251, 392
BstKTI GATC 2 cut(s) 352, 407
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 2 cut(s) 349, 404
BstMWI GCNNNNNNNGC 1 cut(s) 628
BstNI CCWGG 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 196
BstSFI CTRYAG 2 cut(s) 175, 606
BstV2I GAAGAC 2 cut(s) 185, 593
BsuRI GGCC 1 cut(s) 150
BtsCI GGATG 2 cut(s) 251, 392
Cac8I GCNNGC 1 cut(s) 464
Csp6I GTAC 1 cut(s) 437
CviAII CATG 2 cut(s) 347, 383
CviJI RGCY 4 cut(s) 142, 150, 448, 631
CviKI_1 RGCY 4 cut(s) 142, 150, 448, 631
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 4 cut(s) 57, 503, 578, 627
DpnI GATC 2 cut(s) 351, 406
DpnII GATC 2 cut(s) 349, 404
DraI TTTAAA 1 cut(s) 411
Eco57I CTGAAG 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 196
FaeI CATG 2 cut(s) 350, 386
FatI CATG 2 cut(s) 346, 382
FbaI TGATCA 1 cut(s) 349
FokI GGATG 2 cut(s) 258, 399
HaeIII GGCC 1 cut(s) 150
Hin1II CATG 2 cut(s) 350, 386
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 4 cut(s) 5, 52, 163, 574
HphI GGTGA 2 cut(s) 533, 577
Hpy188I TCNGA 2 cut(s) 498, 677
Hpy188III TCNNGA 5 cut(s) 11, 56, 98, 243, 429
HpyAV CCTTC 3 cut(s) 435, 518, 690
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4IV ACGT 2 cut(s) 79, 569
HpyCH4V TGCA 7 cut(s) 171, 177, 326, 390, 513, 657, 665
HpyF10VI GCNNNNNNNGC 1 cut(s) 628
HpyF3I CTNAG 4 cut(s) 57, 503, 578, 627
HpySE526I ACGT 2 cut(s) 79, 569
Hsp92II CATG 2 cut(s) 350, 386
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 2 cut(s) 349, 404
LmnI GCTCC 1 cut(s) 527
LpnPI CCDG 8 cut(s) 69, 132, 183, 210, 228, 254, 414, 515
LweI GCATC 2 cut(s) 236, 377
MaeII ACGT 2 cut(s) 79, 569
MaeIII GTNAC 3 cut(s) 154, 548, 565
MalI GATC 2 cut(s) 351, 406
MboI GATC 2 cut(s) 349, 404
MboII GAAGA 3 cut(s) 61, 185, 593
MfeI CAATTG 1 cut(s) 132
MluCI AATT 7 cut(s) 132, 212, 484, 508, 542, 589, 652
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 3 cut(s) 109, 292, 568
MseI TTAA 5 cut(s) 45, 234, 410, 488, 642
MspR9I CCNGG 1 cut(s) 198
MunI CAATTG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 198
MwoI GCNNNNNNNGC 1 cut(s) 628
NdeII GATC 2 cut(s) 349, 404
NlaIII CATG 2 cut(s) 350, 386
NlaIV GGNNCC 1 cut(s) 202
NmeAIII GCCGAG 1 cut(s) 237
NmuCI GTSAC 2 cut(s) 154, 565
PfeI GAWTC 4 cut(s) 5, 52, 163, 574
Psp1406I AACGTT 1 cut(s) 79
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PspN4I GGNNCC 1 cut(s) 202
PstI CTGCAG 1 cut(s) 179
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
SaqAI TTAA 5 cut(s) 45, 234, 410, 488, 642
Sau3AI GATC 2 cut(s) 349, 404
ScrFI CCNGG 1 cut(s) 198
SetI ASST 6 cut(s) 82, 273, 504, 572, 633, 682
SfaNI GCATC 2 cut(s) 236, 377
SfcI CTRYAG 2 cut(s) 175, 606
SmlI CTYRAG 1 cut(s) 632
SmoI CTYRAG 1 cut(s) 632
Sse9I AATT 7 cut(s) 132, 212, 484, 508, 542, 589, 652
SsiI CCGC 1 cut(s) 460
StyD4I CCNGG 1 cut(s) 196
TaaI ACNGT 1 cut(s) 293
TaiI ACGT 2 cut(s) 82, 572
TasI AATT 7 cut(s) 132, 212, 484, 508, 542, 589, 652
TfiI GAWTC 4 cut(s) 5, 52, 163, 574
Tru1I TTAA 5 cut(s) 45, 234, 410, 488, 642
Tru9I TTAA 5 cut(s) 45, 234, 410, 488, 642
TseFI GTSAC 2 cut(s) 154, 565
Tsp45I GTSAC 2 cut(s) 154, 565
TspDTI ATGAA 1 cut(s) 371
XapI RAATTY 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.