Rw6G000780

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000105
Physical Location & Seq
Forward (+)
1224898 .. 1229157
4260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G000780.1

Sequence Viewer

Length: 3588 bp
ATGTACAAATACGATGTGTTTCTAAGCTTCAGAGGGGAAGATACCCGCGAGACTTTCACCTGCCATCTGTATCGTGCGCTAACTGCGAAAGGAATTATAACCTTCATAGATGACGAAAGCCTGGAGAGAGGATCAACCATTGGCCCATCACTTTTTACAGCAATTCAGGACTCAAGAATCGCCCTTGTCGTTTTTTCAGAGAATTACGCTTCTTCATCCTGGTGCTTGGATGAACTCTCCAAGATTTTTCAATGCTTGGTAAGGAGGGGACGATTCGTCTTCCCGATCTTCTACCGAGTGGCTCCCTCTGATGTGCGCAACCAAACGGGAAGTTTTCAGCTCCGTACAGTAGCGGTAAGGGAACATGAAGAAGTTTATGGAAATAAGGACAAACTAAAGGAGTGGAAAGATGCTTTGACAACGGTTGGCGGTATTGGTGGGTGGGTCTCGGAGGATTATAAGGATGACGGAAAATTGATTGAGGACATTGTTACGTATACACTTCGGAGACTAGGTCATACTTACACCTCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATTTATTAAGCAATTACATATGTCCGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCCATCCATGATCGAATTTGTCAGGATTTTGACCAAAGCTGCATTCTTTCCAATGTTAGAGAAAAATCTGAAAAGGATGGCCTAGTTTCTCTTCAAGAAAAACTACTTTCCAGAATCCTGAGGACAAAAGTTGACATTGAGGATGAATATGCAGGAGCTACTATGATAGAAAGGCGGTTATGTAAACTAAAGGTGCTTGTTGTTATTGATGATGTGGATCACATCAATCAATTAGATAAATTGGCTGGAAGTCGGGACTGGTTCGGTCCTGGGAGTAGAATTATTGTAACCACCCCAGATATTCATTTGCTAAGGGGACATGATGTTGATGCTACATACAAGCCTACTGGGTTAAGTGATGGTGAAGCTATTCAACTATTGAGTTTCAAGGCTTTCAAGAAAAGCTTCCCACCAGAAGATTATTTGGACTTGTGCCACCATATTATAGGGTATGCTCAGGGGCTTCCATTGGCTCTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGACCCAATGAATGGGAAAGTGCAATAGAGAGGCTAAACAATACACCAAATAGACAAGTTATGGATGTGCTTCAGATTAGTTTTGATGGACTAGAGGAAAAAGACCAACAGATATTCCTACATATTGCTTGTTTGTACAAGGGGAAGGACAGGGATCGTGTGACACAAATACTAGGCTATTGCAAGCTAGAACCTGGCATTGGTTTAAGGGTTCTTGAGGAAAAATCTCTCATTACAATTTTTAACAACAAACTGTCGATGCATGATTTGCTACAAGAATTGGCCTTGGAAATAGTTCGTCGAGAGTCCCCTGATGAGCCAGGTAAACGAAGTAGATTATGGTCTCCTGAAGATATCCACAAAGTGCTGAAGAAAAATAAGGGAACAGACAAAATCCGAGCCATGGTAATGGACTTGCCTGAGTTACAAGTGGCTCATTGGAAACCAGAAGCCTTTTCAAATTTGTCTCAACTCAGTCTTCTCCATATTCGTAACGTGGATCTTCCCAAAGGCCTCACTTTTTTATCTAATTCATTGAGGCTCCTTGATTGGAGTGGGTACCCCTTAAGATCCCTCCCACAAAATTTTGAACCGGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTGAACACCTCTGGGAAGGAGCAAAGAATTTTGACAAGTTAAAGTTCATCAAACTATGCCATTCTCAAAAAATTGTCCAGACCCCAGACCTCACAGGCGTTCAAAATCTTGAGACTTTAGATCTTGAAGGATGTAAGACTTTGGTAAAACTTCATCAATCTGTCGGACAACTCAAAAGGCTTATTGTATTGAATCTTAAGGATTGCGAGAGTCTCGAGAATCTGCCAAGTAAGATTGAAATGGAATCTCTTGAAACTTTAATTCTTTCTAACTGCTCCAAAGTTAAAAAGATTCCCGAGTTCGCTGGAAATATGAAGCGTTTATCAAAGCTTTATCTCAATGAGACTGCCATTGAAAAACTGCCTGTGTCAATTGGATGCCTGAGTGGCCTTGCTTCACTGAATCTTAGCAACTGCAAAAATCTTCTATGCCTTGCAAGCACCTTAGAACTGCTTAGTAGATTGGAGTCTCTTAAAAAGCTTGATCTTTCTGGATGTTTGAAACTTGGAGGACAGGATAGTGCGAGGGAGAATGGTGCAATTAGAACTGCTGTAAATCATCTTGTAAAACGTGGTTGTGAAGTTGGGAAATATTTGTCATGGTCCTTGCCTTCTAGATTGGTGCAAAGAGCCTATATGGAGCCAATGAGTATGAAGTTGCCTGTGTCTGATCTGTGTTATTCTACAGAGCAAAATGCAAATACATCAATGAGTATCCGGTTGCCACTATCTCGTCTATGGAATTTAACAGATCTAAACTTAAGTAACTGCAATCTTGGTGACACTGCATTTGCCAACTTTGCCTGTTTTCCCTCTTTAGTGGCACTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGGTCCTGTTTTAAGCTTCAGAACATTAATTTGGAGAATTGCAAGACACTTGAAGAGTTGTCCGGACTTCCATCAAATAGTAAATTGGATGTGAGGGCAGATGGTTGTTCTTCACTGGAAATGCTGTTTGATGTATCCAATTTCAATAGATTGGAGAAATCATATTTCAATTTCATCAATTGCTTCAAACTAAATGACAATCAGGGCTGCTGTAACATAGCATTTGAAATGCTGAGGATATTCCTTAATCAGCAAGTTTCTAGTGTGACGGAAACTTTTCAAATTGTAATTCCTGGAAGTGGAATTCCTGAGTGGTTTGATCATCGAAGATTAGCGAGTTCATTAACTGTAGACCTACTTCCAGATTGGAATGAGAGTAGGTTTTTGGGGTTTGTTTTGTGTGCTGTTTTTGTACTCAATGAGCACCGTCAGGTTGATGAGCTTGATATCCATCAGTTCAAGACTTTTAAGGCCACTCATCATCTTGTATGTTGCCTGAAGCTCAATGGAAGAGAATTAGAAGTATACGGCAAACAGCCTGCATTTCGCTTTAGCGAACAATTTTGCCAGGTTGAGTCAGATCACCTTTGGATATTCTATGTGTCCCGTGATAAATACTTTGGTACAGATTGGTGGCATAACAGTTGCAGTCAACTTGAGTTCTTATTTGAAACAAGAGGGCCAGGCCTGAGGGTGAAGGAGTGTGGGGTACGTCTGATATATGAGCAAGATGTGCTAGAGTTAAACCAAACAATAACTCAATCAAGCAGTAGGATGTCTCCTCCATATGAGGATATATTGACTGATTTTGAGAATCAAGTTGAAGGGGAAACCATTCACCCAAAAAAAAAGTTGAAAGGGGAAACTTGTGGTACTGGTAGTAGAACCTGCACCCTTGAGGAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

1195

Amino Acids

135.69

Weight (kDa)

6.27

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 3 - 184 6.2e-50 TIR domain
TIR_2 PF13676 6 - 102 1.5e-13 TIR domain
NB-ARC PF00931 205 - 359 7.5e-16 NB-ARC domain
AAA_22 PF13401 207 - 305 4.1e-06 AAA domain
WHD_ROQ1 PF23282 425 - 496 5.3e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 685 - 772 2e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 990 - 1138 1.1e-16 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 98, 459
AarI CACCTGC 1 cut(s) 68
Acc16I TGCGCA 1 cut(s) 317
Acc36I ACCTGC 2 cut(s) 68, 3575
Acc65I GGTACC 1 cut(s) 1737
AccB1I GGYRCC 1 cut(s) 1737
AccB7I CCANNNNNTGG 1 cut(s) 1191
AccI GTMKAC 3 cut(s) 497, 3030, 3204
AccII CGCG 1 cut(s) 48
AccIII TCCGGA 1 cut(s) 2741
AciI CCGC 7 cut(s) 46, 353, 429, 568, 602, 643, 847
AclWI GGATC 7 cut(s) 139, 625, 638, 897, 1341, 1686, 1743
AcsI RAATTY 6 cut(s) 687, 1639, 1762, 1837, 2551, 2982
AcuI CTGAAG 6 cut(s) 13, 1235, 1548, 1568, 2681, 3197
AdeI CACNNNGTG 1 cut(s) 1543
AfaI GTAC 8 cut(s) 5, 346, 1316, 1739, 3093, 3304, 3390, 3553
AfiI CCNNNNNNNGG 5 cut(s) 608, 1191, 1379, 1582, 2978
AflII CTTAAG 3 cut(s) 1744, 2006, 2569
AhdI GACNNNNNGTC 1 cut(s) 275
AjnI CCWGG 8 cut(s) 120, 218, 940, 1372, 1498, 2971, 3246, 3361
AjuI GAANNNNNNNTTGG 6 cut(s) 699, 731, 1362, 1394, 2693, 2725
Alw21I GWGCWC 1 cut(s) 3105
AlwI GGATC 7 cut(s) 139, 625, 638, 897, 1341, 1686, 1743
AlwNI CAGNNNCTG 1 cut(s) 2685
Ama87I CYCGRG 3 cut(s) 669, 2024, 2105
Aor13HI TCCGGA 1 cut(s) 2741
AoxI GGCC 8 cut(s) 142, 751, 1461, 1690, 2197, 3150, 3359, 3364
ApeKI GCWGC 3 cut(s) 604, 711, 2886
ApoI RAATTY 6 cut(s) 687, 1639, 1762, 1837, 2551, 2982
ArsI GACNNNNNNTTYG 2 cut(s) 2582, 2614
AseI ATTAAT 1 cut(s) 2706
Asp700I GAANNNNTTC 6 cut(s) 562, 1041, 1076, 1473, 2955, 3513
Asp718I GGTACC 1 cut(s) 1737
AspLEI GCGC 2 cut(s) 79, 318
AspS9I GGNCC 5 cut(s) 143, 938, 2412, 2682, 3359
AsuHPI GGTGA 6 cut(s) 49, 1046, 2600, 3254, 3385, 3509
AvaI CYCGRG 3 cut(s) 669, 2024, 2105
AvaII GGWCC 3 cut(s) 938, 2412, 2682
AxyI CCTNAGG 2 cut(s) 791, 3368
BamHI GGATCC 1 cut(s) 630
BanI GGYRCC 1 cut(s) 1737
BarI GAAGNNNNNNTAC 2 cut(s) 3021, 3053
BbsI GAAGAC 3 cut(s) 271, 1186, 1649
Bbv12I GWGCWC 1 cut(s) 3105
BbvCI CCTCAGC 1 cut(s) 2912
BbvI GCAGC 3 cut(s) 616, 698, 2873
BccI CCATC 9 cut(s) 72, 154, 683, 743, 1025, 1259, 2758, 2774, 3138
BceAI ACGGC 1 cut(s) 3223
BciT130I CCWGG 8 cut(s) 122, 220, 942, 1374, 1500, 2973, 3248, 3363
BciVI GTATCC 2 cut(s) 2534, 2824
BclI TGATCA 1 cut(s) 2998
BfaI CTAG 8 cut(s) 512, 755, 1271, 1352, 1367, 2424, 2940, 3416
BfmI CTRYAG 3 cut(s) 2493, 3027, 3584
BfrI CTTAAG 3 cut(s) 1744, 2006, 2569
BfuAI ACCTGC 2 cut(s) 68, 3575
BfuI GTATCC 2 cut(s) 2534, 2824
BglI GCCNNNNNGGC 1 cut(s) 2196
BglII AGATCT 2 cut(s) 1930, 2560
BisI GCNGC 3 cut(s) 605, 712, 2887
BlsI GCNGC 3 cut(s) 606, 713, 2888
Bme1390I CCNGG 8 cut(s) 122, 220, 942, 1374, 1500, 2973, 3248, 3363
Bme18I GGWCC 3 cut(s) 938, 2412, 2682
BmeRI GACNNNNNGTC 1 cut(s) 275
BmeT110I CYCGRG 3 cut(s) 669, 2024, 2105
BmgT120I GGNCC 5 cut(s) 143, 938, 2412, 2682, 3359
BmiI GGNNCC 5 cut(s) 303, 632, 1721, 1739, 2451
BmrFI CCNGG 8 cut(s) 122, 220, 942, 1374, 1500, 2973, 3248, 3363
BmrI ACTGGG 1 cut(s) 1029
BmsI GCATC 5 cut(s) 400, 991, 1428, 2177, 2685
BmuI ACTGGG 1 cut(s) 1029
BpiI GAAGAC 3 cut(s) 271, 1186, 1649
BpmI CTGGAG 1 cut(s) 143
Bpu10I CCTNAGC 3 cut(s) 983, 1128, 2912
BpuEI CTTGAG 4 cut(s) 157, 1415, 1940, 3356
BsaAI YACGTR 1 cut(s) 495
BsaBI GATNNNNATC 2 cut(s) 888, 3129
BsaI GGTCTC 2 cut(s) 451, 1527
BsaJI CCNNGG 4 cut(s) 634, 941, 1464, 1581
BsaWI WCCGGW 3 cut(s) 1771, 2526, 2741
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bsc4I CCNNNNNNNGG 5 cut(s) 608, 1191, 1379, 1582, 2978
Bse1I ACTGG 4 cut(s) 935, 1024, 2799, 3559
Bse21I CCTNAGG 2 cut(s) 791, 3368
Bse8I GATNNNNATC 2 cut(s) 888, 3129
BseAI TCCGGA 1 cut(s) 2741
BseBI CCWGG 8 cut(s) 122, 220, 942, 1374, 1500, 2973, 3248, 3363
BseDI CCNNGG 4 cut(s) 634, 941, 1464, 1581
BseJI GATNNNNATC 2 cut(s) 888, 3129
BseLI CCNNNNNNNGG 5 cut(s) 608, 1191, 1379, 1582, 2978
BseMII CTCAG 9 cut(s) 782, 1142, 1590, 1666, 2183, 2633, 2903, 2979, 3359
BseNI ACTGG 4 cut(s) 935, 1024, 2799, 3559
BseRI GAGGAG 1 cut(s) 3450
BseXI GCAGC 3 cut(s) 616, 698, 2873
BsgI GTGCAG 1 cut(s) 3553
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 8 cut(s) 144, 753, 1463, 1692, 2199, 3152, 3361, 3366
BshNI GGYRCC 1 cut(s) 1737
BsiHKAI GWGCWC 1 cut(s) 3105
BsiHKCI CYCGRG 3 cut(s) 669, 2024, 2105
BsiSI CCGG 3 cut(s) 1772, 2527, 2742
BslFI GGGAC 5 cut(s) 282, 941, 1002, 1471, 3268
BslI CCNNNNNNNGG 5 cut(s) 608, 1191, 1379, 1582, 2978
BsmFI GGGAC 5 cut(s) 282, 941, 1002, 1471, 3268
BsmI GAATGC 1 cut(s) 714
BsnI GGCC 8 cut(s) 144, 753, 1463, 1692, 2199, 3152, 3361, 3366
Bso31I GGTCTC 2 cut(s) 451, 1527
BsoBI CYCGRG 3 cut(s) 669, 2024, 2105
Bsp1286I GDGCHC 1 cut(s) 3105
Bsp13I TCCGGA 1 cut(s) 2741
Bsp1407I TGTACA 2 cut(s) 3, 1314
Bsp19I CCATGG 2 cut(s) 634, 1581
BspACI CCGC 7 cut(s) 46, 353, 429, 568, 602, 643, 847
BspANI GGCC 8 cut(s) 144, 753, 1463, 1692, 2199, 3152, 3361, 3366
BspCNI CTCAG 9 cut(s) 783, 1141, 1591, 1665, 2184, 2634, 2904, 2980, 3360
BspEI TCCGGA 1 cut(s) 2741
BspFNI CGCG 1 cut(s) 48
BspLI GGNNCC 5 cut(s) 303, 632, 1721, 1739, 2451
BspMI ACCTGC 2 cut(s) 68, 3575
BspPI GGATC 7 cut(s) 139, 625, 638, 897, 1341, 1686, 1743
BspT107I GGYRCC 1 cut(s) 1737
BspTI CTTAAG 3 cut(s) 1744, 2006, 2569
BspTNI GGTCTC 2 cut(s) 451, 1527
BsrGI TGTACA 2 cut(s) 3, 1314
BsrI ACTGG 4 cut(s) 935, 1024, 2799, 3559
BssECI CCNNGG 4 cut(s) 634, 941, 1464, 1581
BssNAI GTATAC 2 cut(s) 498, 3205
BssT1I CCWWGG 3 cut(s) 634, 1464, 1581
Bst1107I GTATAC 2 cut(s) 498, 3205
Bst2UI CCWGG 8 cut(s) 122, 220, 942, 1374, 1500, 2973, 3248, 3363
Bst4CI ACNGT 6 cut(s) 349, 424, 1434, 3028, 3107, 3323
Bst6I CTCTTC 4 cut(s) 535, 768, 2727, 3184
BstAFI CTTAAG 3 cut(s) 1744, 2006, 2569
BstAPI GCANNNNNTGC 2 cut(s) 1447, 3412
BstAUI TGTACA 2 cut(s) 3, 1314
BstBAI YACGTR 1 cut(s) 495
BstC8I GCNNGC 4 cut(s) 641, 1364, 2248, 3219
BstDSI CCRYGG 2 cut(s) 634, 1581
BstFNI CGCG 1 cut(s) 48
BstHHI GCGC 2 cut(s) 79, 318
BstMWI GCNNNNNNNGC 7 cut(s) 83, 645, 1447, 2196, 2247, 2609, 3412
BstNI CCWGG 8 cut(s) 122, 220, 942, 1374, 1500, 2973, 3248, 3363
BstNSI RCATGY 1 cut(s) 643
BstSCI CCNGG 8 cut(s) 120, 218, 940, 1372, 1498, 2971, 3246, 3361
BstSFI CTRYAG 3 cut(s) 2493, 3027, 3584
BstSNI TACGTA 1 cut(s) 495
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 3 cut(s) 616, 698, 2873
BstV2I GAAGAC 3 cut(s) 271, 1186, 1649
BstX2I RGATCY 5 cut(s) 630, 1678, 1748, 1930, 2560
BstXI CCANNNNNNTGG 1 cut(s) 1588
BstYI RGATCY 5 cut(s) 630, 1678, 1748, 1930, 2560
BstZ17I GTATAC 2 cut(s) 498, 3205
Bsu36I CCTNAGG 2 cut(s) 791, 3368
BsuI GTATCC 2 cut(s) 2534, 2824
BsuRI GGCC 8 cut(s) 144, 753, 1463, 1692, 2199, 3152, 3361, 3366
BtgI CCRYGG 2 cut(s) 634, 1581
BtsI GCAGTG 1 cut(s) 2592
BtsIMutI CAGTG 3 cut(s) 2207, 2592, 2792
BveI ACCTGC 2 cut(s) 68, 3575
Cac8I GCNNGC 4 cut(s) 641, 1364, 2248, 3219
CaiI CAGNNNCTG 1 cut(s) 2685
CfoI GCGC 2 cut(s) 79, 318
Cfr13I GGNCC 5 cut(s) 143, 938, 2412, 2682, 3359
Csp6I GTAC 8 cut(s) 4, 345, 1315, 1738, 3092, 3303, 3389, 3552
CviAII CATG 8 cut(s) 365, 635, 640, 680, 992, 1442, 1582, 2409
CviQI GTAC 8 cut(s) 4, 345, 1315, 1738, 3092, 3303, 3389, 3552
DraIII CACNNNGTG 1 cut(s) 1543
DriI GACNNNNNGTC 1 cut(s) 275
Eam1104I CTCTTC 4 cut(s) 535, 768, 2727, 3184
Eam1105I GACNNNNNGTC 1 cut(s) 275
EarI CTCTTC 4 cut(s) 535, 768, 2727, 3184
Eco105I TACGTA 1 cut(s) 495
Eco130I CCWWGG 3 cut(s) 634, 1464, 1581
Eco147I AGGCCT 2 cut(s) 1692, 3366
Eco31I GGTCTC 2 cut(s) 451, 1527
Eco32I GATATC 2 cut(s) 1534, 3127
Eco47I GGWCC 3 cut(s) 938, 2412, 2682
Eco57I CTGAAG 6 cut(s) 13, 1235, 1548, 1568, 2681, 3197
Eco81I CCTNAGG 2 cut(s) 791, 3368
Eco88I CYCGRG 3 cut(s) 669, 2024, 2105
EcoO109I RGGNCCY 1 cut(s) 2682
EcoRI GAATTC 1 cut(s) 2982
EcoRII CCWGG 8 cut(s) 120, 218, 940, 1372, 1498, 2971, 3246, 3361
EcoRV GATATC 2 cut(s) 1534, 3127
EcoT14I CCWWGG 3 cut(s) 634, 1464, 1581
EcoT22I ATGCAT 1 cut(s) 1443
ErhI CCWWGG 3 cut(s) 634, 1464, 1581
FaeI CATG 8 cut(s) 368, 638, 643, 683, 995, 1445, 1585, 2412
FalI AAGNNNNNCTT 2 cut(s) 1061, 1093
FaqI GGGAC 5 cut(s) 282, 941, 1002, 1471, 3268
FatI CATG 8 cut(s) 364, 634, 639, 679, 991, 1441, 1581, 2408
FauI CCCGC 3 cut(s) 53, 575, 636
FauNDI CATATG 2 cut(s) 596, 3466
FbaI TGATCA 1 cut(s) 2998
FblI GTMKAC 3 cut(s) 497, 3030, 3204
Fnu4HI GCNGC 3 cut(s) 605, 712, 2887
Fsp4HI GCNGC 3 cut(s) 605, 712, 2887
FspBI CTAG 8 cut(s) 512, 755, 1271, 1352, 1367, 2424, 2940, 3416
FspI TGCGCA 1 cut(s) 317
GlaI GCGC 2 cut(s) 78, 317
GluI GCNGC 3 cut(s) 605, 712, 2887
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 8 cut(s) 144, 753, 1463, 1692, 2199, 3152, 3361, 3366
HapII CCGG 3 cut(s) 1772, 2527, 2742
HhaI GCGC 2 cut(s) 79, 318
Hin1II CATG 8 cut(s) 368, 638, 643, 683, 995, 1445, 1585, 2412
Hin6I GCGC 2 cut(s) 77, 316
HinP1I GCGC 2 cut(s) 77, 316
HincII GTYRAC 3 cut(s) 541, 805, 3332
HindII GTYRAC 3 cut(s) 541, 805, 3332
HindIII AAGCTT 6 cut(s) 25, 1075, 2138, 2288, 2663, 2693
HpaII CCGG 3 cut(s) 1772, 2527, 2742
HphI GGTGA 6 cut(s) 49, 1046, 2600, 3254, 3385, 3509
Hpy166II GTNNAC 8 cut(s) 498, 541, 805, 857, 1505, 3031, 3205, 3332
Hpy8I GTNNAC 8 cut(s) 498, 541, 805, 857, 1505, 3031, 3205, 3332
Hpy99I CGWCG 1 cut(s) 1482
HpyAV CCTTC 7 cut(s) 112, 1318, 1820, 1931, 2430, 3370, 3497
HpyCH4III ACNGT 6 cut(s) 349, 424, 1434, 3028, 3107, 3323
HpyCH4IV ACGT 4 cut(s) 494, 1674, 2380, 3391
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 645, 1447, 2196, 2247, 2609, 3412
HpySE526I ACGT 4 cut(s) 494, 1674, 2380, 3391
Hsp92II CATG 8 cut(s) 368, 638, 643, 683, 995, 1445, 1585, 2412
HspAI GCGC 2 cut(s) 77, 316
Kpn2I TCCGGA 1 cut(s) 2741
KpnI GGTACC 1 cut(s) 1741
Ksp22I TGATCA 1 cut(s) 2998
LmnI GCTCC 7 cut(s) 307, 345, 827, 1725, 1829, 2090, 2449
Lsp1109I GCAGC 3 cut(s) 616, 698, 2873
LweI GCATC 5 cut(s) 400, 991, 1428, 2177, 2685
MaeI CTAG 8 cut(s) 512, 755, 1271, 1352, 1367, 2424, 2940, 3416
MaeII ACGT 4 cut(s) 494, 1674, 2380, 3391
MaeIII GTNAC 9 cut(s) 490, 958, 1339, 1602, 1670, 2573, 2588, 2891, 2944
MfeI CAATTG 2 cut(s) 2181, 2857
MflI RGATCY 5 cut(s) 630, 1678, 1748, 1930, 2560
MhlI GDGCHC 1 cut(s) 3105
MlyI GAGTC 5 cut(s) 164, 1493, 2029, 2285, 3263
MmeI TCCRAC 1 cut(s) 1954
Mph1103I ATGCAT 1 cut(s) 1443
MroI TCCGGA 1 cut(s) 2741
MroXI GAANNNNTTC 6 cut(s) 562, 1041, 1076, 1473, 2955, 3513
MslI CAYNNNNRTG 3 cut(s) 220, 557, 1586
MspCI CTTAAG 3 cut(s) 1744, 2006, 2569
MspI CCGG 3 cut(s) 1772, 2527, 2742
MspR9I CCNGG 8 cut(s) 122, 220, 942, 1374, 1500, 2973, 3248, 3363
MunI CAATTG 2 cut(s) 2181, 2857
Mva1269I GAATGC 1 cut(s) 714
MvaI CCWGG 8 cut(s) 122, 220, 942, 1374, 1500, 2973, 3248, 3363
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 7 cut(s) 83, 645, 1447, 2196, 2247, 2609, 3412
NcoI CCATGG 2 cut(s) 634, 1581
NdeI CATATG 2 cut(s) 596, 3466
NlaIII CATG 8 cut(s) 368, 638, 643, 683, 995, 1445, 1585, 2412
NlaIV GGNNCC 5 cut(s) 303, 632, 1721, 1739, 2451
NmuCI GTSAC 3 cut(s) 1339, 2588, 2944
NsbI TGCGCA 1 cut(s) 317
NsiI ATGCAT 1 cut(s) 1443
NspI RCATGY 1 cut(s) 643
PaeI GCATGC 1 cut(s) 643
PaeR7I CTCGAG 2 cut(s) 669, 2024
PaqCI CACCTGC 1 cut(s) 68
PceI AGGCCT 2 cut(s) 1692, 3366
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PctI GAATGC 1 cut(s) 714
PdmI GAANNNNTTC 6 cut(s) 562, 1041, 1076, 1473, 2955, 3513
PflFI GACNNNGTC 1 cut(s) 513
PflMI CCANNNNNTGG 1 cut(s) 1191
PfoI TCCNGGA 1 cut(s) 2971
PkrI GCNGC 3 cut(s) 606, 713, 2888
PleI GAGTC 5 cut(s) 164, 1492, 2028, 2284, 3262
PpsI GAGTC 5 cut(s) 164, 1492, 2028, 2284, 3262
Ppu21I YACGTR 1 cut(s) 495
PpuMI RGGWCCY 1 cut(s) 2682
PshBI ATTAAT 1 cut(s) 2706
PsiI TTATAA 2 cut(s) 98, 459
Psp5II RGGWCCY 1 cut(s) 2682
Psp6I CCWGG 8 cut(s) 120, 218, 940, 1372, 1498, 2971, 3246, 3361
PspGI CCWGG 8 cut(s) 120, 218, 940, 1372, 1498, 2971, 3246, 3361
PspN4I GGNNCC 5 cut(s) 303, 632, 1721, 1739, 2451
PspPI GGNCC 5 cut(s) 143, 938, 2412, 2682, 3359
PspPPI RGGWCCY 1 cut(s) 2682
PspXI VCTCGAGB 1 cut(s) 669
PstNI CAGNNNCTG 1 cut(s) 2685
PsuI RGATCY 5 cut(s) 630, 1678, 1748, 1930, 2560
PsyI GACNNNGTC 1 cut(s) 513
RsaI GTAC 8 cut(s) 5, 346, 1316, 1739, 3093, 3304, 3390, 3553
RsaNI GTAC 8 cut(s) 4, 345, 1315, 1738, 3092, 3303, 3389, 3552
RseI CAYNNNNRTG 3 cut(s) 220, 557, 1586
SatI GCNGC 3 cut(s) 605, 712, 2887
Sau96I GGNCC 5 cut(s) 143, 938, 2412, 2682, 3359
SchI GAGTC 5 cut(s) 164, 1493, 2029, 2285, 3263
ScrFI CCNGG 8 cut(s) 122, 220, 942, 1374, 1500, 2973, 3248, 3363
SduI GDGCHC 1 cut(s) 3105
SfaNI GCATC 5 cut(s) 400, 991, 1428, 2177, 2685
SfcI CTRYAG 3 cut(s) 2493, 3027, 3584
Sfr274I CTCGAG 2 cut(s) 669, 2024
SinI GGWCC 3 cut(s) 938, 2412, 2682
SlaI CTCGAG 2 cut(s) 669, 2024
SmiMI CAYNNNNRTG 3 cut(s) 220, 557, 1586
SnaBI TACGTA 1 cut(s) 495
SphI GCATGC 1 cut(s) 643
SseBI AGGCCT 2 cut(s) 1692, 3366
SsiI CCGC 7 cut(s) 46, 353, 429, 568, 602, 643, 847
SspI AATATT 1 cut(s) 2402
SspMI CTAG 8 cut(s) 512, 755, 1271, 1352, 1367, 2424, 2940, 3416
StuI AGGCCT 2 cut(s) 1692, 3366
StyD4I CCNGG 8 cut(s) 120, 218, 940, 1372, 1498, 2971, 3246, 3361
StyI CCWWGG 3 cut(s) 634, 1464, 1581
TaaI ACNGT 6 cut(s) 349, 424, 1434, 3028, 3107, 3323
TaiI ACGT 4 cut(s) 497, 1677, 2383, 3394
TaqI TCGA 6 cut(s) 670, 685, 1436, 1480, 2025, 3004
TaqII GACCGA 1 cut(s) 926
TatI WGTACW 3 cut(s) 3, 1314, 3091
TscAI CASTG 3 cut(s) 2214, 2599, 2799
TseFI GTSAC 3 cut(s) 1339, 2588, 2944
TseI GCWGC 3 cut(s) 604, 711, 2886
Tsp45I GTSAC 3 cut(s) 1339, 2588, 2944
TspGWI ACGGA 3 cut(s) 332, 483, 2963
TspRI CASTG 3 cut(s) 2214, 2599, 2799
Tth111I GACNNNGTC 1 cut(s) 513
Van91I CCANNNNNTGG 1 cut(s) 1191
Vha464I CTTAAG 3 cut(s) 1744, 2006, 2569
VpaK11BI GGWCC 3 cut(s) 938, 2412, 2682
VspI ATTAAT 1 cut(s) 2706
XapI RAATTY 6 cut(s) 687, 1639, 1762, 1837, 2551, 2982
XbaI TCTAGA 1 cut(s) 2423
XceI RCATGY 1 cut(s) 643
XhoI CTCGAG 2 cut(s) 669, 2024
XmiI GTMKAC 3 cut(s) 497, 3030, 3204
XmnI GAANNNNTTC 6 cut(s) 562, 1041, 1076, 1473, 2955, 3513
XspI CTAG 8 cut(s) 512, 755, 1271, 1352, 1367, 2424, 2940, 3416
Zsp2I ATGCAT 1 cut(s) 1443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.