RchiOBHm_Chr1g0322941

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Reverse (-)
10446584 .. 10454430
7847 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55290

Sequence Viewer

Length: 3582 bp
ATGGCGTCAACATCTTCTCGTCGTCAATGGAAATACGATGCGTTTTTAAGTTTCAGGGGGGAAGATACGCGCCTAAGTTTTACCGATTCTCTGTATTCTGCACTAAATCAGAGAGGAATTCTCACCTTTCTGGATGACCGAGAGCTTGAGAGAGGAAAATCCATTAAGCCTGAACTTTTAGCTGCAATTGAGGAGTCTAGATCTGCGATTGTCATTCTCTCACTCAACTATGCTTCTTCGACATGGTGCTTGGATGAACTCGTCAAGATTATTCAGTGCATGAAAGAGATGGGCCAACAAGTCCTCCCCGTCTTCTTCTGGGTGGATCCTTCTGATGTGCGGCACCAAAGGGGAAGTTTTGAGCTCAAAAGGAAACACCAAGTAGATGTAGAAGTAAGGGAGCATGAAGAAGTTTATGGCAAGAAGGACAGACTAAATGCGTGGAGAGCTGCTTTGACAGAGGTCGCCGATATTTCTGGCTGGCATCCCAAGAATTATGTATATCAAACACAACTAATTGAGGAAATTGTTAGTCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTCATGATTTATTAAGCAATTACATATGTCCACACCTTTGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCTATGATGAAATTTGTCAGGATTTTGACCGAAGCTGCTTTCTTTCTAATGTTAGAGAAATGTCTAAAAACAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAAAATCCTGATGGCAGAAATTAAAAATATAGAGGATGAATACACAGGAGCCGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAACTAGAGAAATTGGCTAGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATCATCATAACCACCACAGATATTCACTTGTTAAAGGCACATGGTGTTGATTCTAAATACAAGGCTAACGGGTTAAATTATGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATTTAACCCACCAGAAGATTATTTGCATCTGTGCTACGATATTTTAGGGAAGGCTCAGGGACTTCCGTTGGCTCTGGTGGTTTTAGGGTCTTTTCTATTTGATAGAAGTGCTGATGAATGGGAAAGTGCAATACGTAGGCTAAAGAACACACCTGATAAATGCATTATTGACGTGCTTCGAATTAGTCTTGATGGACTGAATAAGGAAGAGAAAGAAATATTCCTACATATTGCTTGCTTTTACAAAGGGAAGGATAAGGATCGTGTGACACAAATACTAGGCTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAAATCTCTCATAACTATCTCCAACAACGAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAAGAGTCTCCTGAAGAGCCAGGCAAACGTAGTAGATTATGGTTTCATAAAGAGATCAACAATATGCTGAAGAAAAATAAGGGCACAGGTTCAATCAAAGGCATGGTAATGGAGTTGACTAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTTGTAATGTGGACCTTCCCGAAGGCCTCACTTGTCTTTCTAATTCGTTGAAACTGCTCGAATGGACTGGGTATCCCTTAAGATCTCTCCCAGAAAATTTTGAAGCAGATGAACTTATTGAACTTAACCTGTGCCACAGCAACATTGAACAGCTTTGGAAGGGAACAAAGAATTTTGACGAGTTGAAGTTCATCAAACTCTGCCATTCTAAAAACATTTCGGAGACCCCAGACCTCACAGGCGTTCAGAATCTTGAGAGTTTAGATCTACAAGGGTGTGAGAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCGAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCCAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTCGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGTGTCAATTGAACAGCTGAGCGGCCTTGTGTCATTGAATCTGAGCAACTGCAGAAATCTTGCCCGTCTTCCGAGTACCATCAATAAATTGAAGTCTATTAAAGATCTTAATCTTTCTGGATGCTCGAAACTCGGCAATCATCAGGTAAATGTGCGGGAGATGGACTGTTTTGAGGAAAATGATGTGAATAGTGGGTCTGCAATAGAAATGTCAACTACCCACGATCTCATAAACTATGTGAGAGGTTCAACCTTTCATGGATGTGAAGTTGTTTGGGGATCTTCAAATAAGTTCTTGTCTTCTAGATTGGTGCAAAAAGTGAATACAGAGCCAGTGAGTTGCCGCTTGCCTATATCTGGTCTCTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGAATTTGCCATCGAATTTGGTAACTTTCCCTTTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGTCTTCCTTCAGGCATTGGTTTGCTTTCTAAGCTTGAGAAACTTAAGTTGGAGAATTGCAAGAGACTTCAGGAGTTGTCAGACCTTCCATCAAATAGTATGCTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGAAGCAATAACATAGCATTCGAAATGCTGAAGACATTCATGTCTCAGGGAATCTTTAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCGGAGTGTTCGTTGTTACTTAAGTGTATCTCTACCTGCACATTGGAATAACAGTCGGTTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATGGAGTGGATGAGCCTTATATAGATGAATTCGAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCGATGGAAGAGAGTTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAGGAATTTTGCCAGGTTGATTTAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCGATTTGAGTTCTTATTTGAAACCAGAGGGCCGGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGGAAGATGTGCAAGAGTTGAACCAAACAACCACTCAATCAAGCAGTGGGATGTCTCCTTATGAGGATTTATTGATTGGTTTTGACATTCCAGTTGGAGGGGAAACCAGTGGCACTGGTAGCAGAACTTGCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1193

Amino Acids

135.72

Weight (kDa)

5.79

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 190 9.4e-51 TIR domain
TIR_2 PF13676 15 - 129 7.5e-14 TIR domain
NB-ARC PF00931 215 - 370 1.8e-19 NB-ARC domain
NACHT PF05729 220 - 339 9.1e-07 NACHT domain
WHD_ROQ1 PF23282 437 - 508 2.3e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 606 - 624 3.2e-06 Leucine Rich Repeat
LRR_13 PF23286 697 - 782 1e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 708 - 778 1.6e-09 Leucine-rich repeat region
LRR_14 PF23598 855 - 955 3.3e-07 Leucine-rich repeat region
C-JID PF20160 1000 - 1149 7.4e-19 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2953
Acc36I ACCTGC 1 cut(s) 3076
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 1 cut(s) 3142
AccBSI CCGCTC 1 cut(s) 2232
AccI GTMKAC 1 cut(s) 557
AccII CGCG 1 cut(s) 70
AciI CCGC 8 cut(s) 340, 601, 676, 867, 883, 2232, 2365, 2554
AclI AACGTT 1 cut(s) 2164
AclWI GGATC 7 cut(s) 320, 333, 658, 671, 933, 1377, 2497
AcuI CTGAAG 7 cut(s) 1548, 1604, 2685, 2744, 2963, 3227, 3419
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 1031
AfaI GTAC 4 cut(s) 2287, 2818, 3123, 3334
AfiI CCNNNNNNNGG 5 cut(s) 646, 1415, 2567, 3142, 3530
AflII CTTAAG 3 cut(s) 1780, 2735, 3052
AjiI CACGTC 1 cut(s) 1282
AjnI CCWGG 4 cut(s) 976, 1534, 3001, 3276
AjuI GAANNNNNNNTTGG 2 cut(s) 2723, 2755
AleI CACNNNNGTG 1 cut(s) 642
Alw21I GWGCWC 2 cut(s) 366, 3135
AlwI GGATC 7 cut(s) 320, 333, 658, 671, 933, 1377, 2497
Ama87I CYCGRG 3 cut(s) 702, 2060, 2141
AoxI GGCC 5 cut(s) 292, 784, 1726, 2233, 3389
ApeKI GCWGC 3 cut(s) 182, 449, 744
AseI ATTAAT 1 cut(s) 2105
Asp700I GAANNNNTTC 6 cut(s) 595, 807, 1077, 1509, 2948, 2985
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 4 cut(s) 292, 974, 1714, 3389
AsuC2I CCSGG 1 cut(s) 3393
AsuHPI GGTGA 5 cut(s) 115, 2627, 2671, 3284, 3415
AsuII TTCGAA 2 cut(s) 1288, 2934
AvaI CYCGRG 3 cut(s) 702, 2060, 2141
AvaII GGWCC 2 cut(s) 974, 1714
BaeGI GKGCMC 1 cut(s) 1601
BamHI GGATCC 2 cut(s) 325, 663
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 3315
BbsI GAAGAC 6 cut(s) 304, 1685, 2270, 2502, 2687, 2951
Bbv12I GWGCWC 2 cut(s) 366, 3135
BbvI GCAGC 3 cut(s) 169, 436, 731
BcgI CGANNNNNNTGC 2 cut(s) 2041, 2075
BciT130I CCWGG 4 cut(s) 978, 1536, 3003, 3278
BciVI GTATCC 1 cut(s) 1785
BcnI CCSGG 1 cut(s) 3393
BfaI CTAG 7 cut(s) 198, 788, 941, 954, 1388, 2514, 2795
BfmI CTRYAG 1 cut(s) 2260
BfrI CTTAAG 3 cut(s) 1780, 2735, 3052
BfuAI ACCTGC 1 cut(s) 3076
BfuI GTATCC 1 cut(s) 1785
BglII AGATCT 4 cut(s) 200, 1784, 1966, 2314
BisI GCNGC 7 cut(s) 183, 341, 450, 745, 867, 2233, 2554
BlpI GCTNAGC 1 cut(s) 2228
BlsI GCNGC 7 cut(s) 184, 342, 451, 746, 868, 2234, 2555
Bme1390I CCNGG 5 cut(s) 978, 1536, 3003, 3278, 3393
Bme18I GGWCC 2 cut(s) 974, 1714
BmeT110I CYCGRG 3 cut(s) 702, 2060, 2141
BmgBI CACGTC 1 cut(s) 1282
BmgT120I GGNCC 4 cut(s) 292, 974, 1714, 3389
BmiI GGNNCC 4 cut(s) 327, 344, 665, 865
BmrFI CCNGG 5 cut(s) 978, 1536, 3003, 3278, 3393
BmrI ACTGGG 1 cut(s) 1779
BmsI GCATC 7 cut(s) 28, 493, 1144, 1464, 2321, 2830, 2852
BmuI ACTGGG 1 cut(s) 1779
BoxI GACNNNNGTC 1 cut(s) 461
BpiI GAAGAC 6 cut(s) 304, 1685, 2270, 2502, 2687, 2951
BplI GAGNNNNNCTC 2 cut(s) 105, 137
Bpu10I CCTNAGC 1 cut(s) 1164
Bpu1102I GCTNAGC 1 cut(s) 2228
Bpu14I TTCGAA 2 cut(s) 1288, 2934
BpuEI CTTGAG 3 cut(s) 167, 1976, 2747
BpuMI CCSGG 1 cut(s) 3393
BsaAI YACGTR 1 cut(s) 1244
BsaBI GATNNNNATC 4 cut(s) 924, 1368, 2319, 3288
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1919, 2576
BsaJI CCNNGG 4 cut(s) 667, 977, 2008, 3136
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 3028, 3058
Bsc4I CCNNNNNNNGG 5 cut(s) 646, 1415, 2567, 3142, 3530
Bse1I ACTGG 6 cut(s) 971, 1774, 2543, 3524, 3540, 3553
Bse8I GATNNNNATC 4 cut(s) 924, 1368, 2319, 3288
BseBI CCWGG 4 cut(s) 978, 1536, 3003, 3278
BseDI CCNNGG 4 cut(s) 667, 977, 2008, 3136
BseGI GGATG 8 cut(s) 139, 259, 484, 856, 2336, 2477, 3151, 3489
BseJI GATNNNNATC 4 cut(s) 924, 1368, 2319, 3288
BseLI CCNNNNNNNGG 5 cut(s) 646, 1415, 2567, 3142, 3530
BseMII CTCAG 6 cut(s) 1178, 2219, 2243, 2588, 2972, 3009
BseNI ACTGG 6 cut(s) 971, 1774, 2543, 3524, 3540, 3553
BseRI GAGGAG 1 cut(s) 206
BseSI GKGCMC 1 cut(s) 1601
BseXI GCAGC 3 cut(s) 169, 436, 731
BseYI CCCAGC 1 cut(s) 1500
BsgI GTGCAG 2 cut(s) 84, 3054
Bsh1236I CGCG 1 cut(s) 70
BshFI GGCC 5 cut(s) 294, 786, 1728, 2235, 3391
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 2 cut(s) 366, 3135
BsiHKCI CYCGRG 3 cut(s) 702, 2060, 2141
BsiSI CCGG 1 cut(s) 3392
BslFI GGGAC 1 cut(s) 1182
BslI CCNNNNNNNGG 5 cut(s) 646, 1415, 2567, 3142, 3530
BsmFI GGGAC 1 cut(s) 1182
BsmI GAATGC 2 cut(s) 603, 2930
BsnI GGCC 5 cut(s) 294, 786, 1728, 2235, 3391
Bso31I GGTCTC 2 cut(s) 1919, 2576
BsoBI CYCGRG 3 cut(s) 702, 2060, 2141
Bsp119I TTCGAA 2 cut(s) 1288, 2934
Bsp1286I GDGCHC 3 cut(s) 366, 1601, 3135
Bsp1720I GCTNAGC 1 cut(s) 2228
Bsp19I CCATGG 2 cut(s) 667, 3136
BspACI CCGC 8 cut(s) 340, 601, 676, 867, 883, 2232, 2365, 2554
BspANI GGCC 5 cut(s) 294, 786, 1728, 2235, 3391
BspCNI CTCAG 6 cut(s) 1177, 2220, 2244, 2589, 2971, 3010
BspFNI CGCG 1 cut(s) 70
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 4 cut(s) 327, 344, 665, 865
BspMAI CTGCAG 1 cut(s) 2264
BspMI ACCTGC 1 cut(s) 3076
BspPI GGATC 7 cut(s) 320, 333, 658, 671, 933, 1377, 2497
BspQI GCTCTTC 1 cut(s) 1524
BspT104I TTCGAA 2 cut(s) 1288, 2934
BspT107I GGYRCC 1 cut(s) 342
BspTI CTTAAG 3 cut(s) 1780, 2735, 3052
BspTNI GGTCTC 2 cut(s) 1919, 2576
BsrBI CCGCTC 1 cut(s) 2232
BsrI ACTGG 6 cut(s) 971, 1774, 2543, 3524, 3540, 3553
BssECI CCNNGG 4 cut(s) 667, 977, 2008, 3136
BssNAI GTATAC 1 cut(s) 558
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 3315
BssT1I CCWWGG 3 cut(s) 667, 2008, 3136
Bst1107I GTATAC 1 cut(s) 558
Bst2BI CACGAG 1 cut(s) 3315
Bst2UI CCWGG 4 cut(s) 978, 1536, 3003, 3278
Bst4CI ACNGT 4 cut(s) 1470, 2378, 3086, 3353
Bst6I CTCTTC 4 cut(s) 1311, 1512, 1524, 3214
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 3 cut(s) 1780, 2735, 3052
BstAPI GCANNNNNTGC 2 cut(s) 1483, 3561
BstBAI YACGTR 1 cut(s) 1244
BstBI TTCGAA 2 cut(s) 1288, 2934
BstC8I GCNNGC 7 cut(s) 482, 674, 1345, 1400, 2558, 3249, 3566
BstDSI CCRYGG 2 cut(s) 667, 3136
BstEII GGTNACC 1 cut(s) 2659
BstF5I GGATG 8 cut(s) 139, 259, 484, 856, 2336, 2477, 3151, 3489
BstFNI CGCG 1 cut(s) 70
BstHHI GCGC 1 cut(s) 72
BstMWI GCNNNNNNNGC 7 cut(s) 446, 678, 1483, 2722, 3110, 3552, 3561
BstNI CCWGG 4 cut(s) 978, 1536, 3003, 3278
BstNSI RCATGY 1 cut(s) 676
BstPAI GACNNNNGTC 1 cut(s) 461
BstPI GGTNACC 1 cut(s) 2659
BstSCI CCNGG 5 cut(s) 976, 1534, 3001, 3276, 3391
BstSFI CTRYAG 1 cut(s) 2260
BstSLI GKGCMC 1 cut(s) 1601
BstSNI TACGTA 1 cut(s) 1244
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 3 cut(s) 169, 436, 731
BstV2I GAAGAC 6 cut(s) 304, 1685, 2270, 2502, 2687, 2951
BstX2I RGATCY 7 cut(s) 200, 325, 663, 1784, 1966, 2314, 2489
BstYI RGATCY 7 cut(s) 200, 325, 663, 1784, 1966, 2314, 2489
BstZ17I GTATAC 1 cut(s) 558
BsuI GTATCC 1 cut(s) 1785
BsuRI GGCC 5 cut(s) 294, 786, 1728, 2235, 3391
BtgI CCRYGG 2 cut(s) 667, 3136
BtrI CACGTC 1 cut(s) 1282
BtsCI GGATG 8 cut(s) 139, 259, 484, 856, 2336, 2477, 3151, 3489
BtsI GCAGTG 1 cut(s) 3484
BtsIMutI CAGTG 6 cut(s) 281, 2550, 2822, 3484, 3546, 3547
BveI ACCTGC 1 cut(s) 3076
Cac8I GCNNGC 7 cut(s) 482, 674, 1345, 1400, 2558, 3249, 3566
CciI TCATGA 1 cut(s) 607
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 4 cut(s) 292, 974, 1714, 3389
Csp6I GTAC 4 cut(s) 2286, 2817, 3122, 3333
CviQI GTAC 4 cut(s) 2286, 2817, 3122, 3333
DraIII CACNNNGTG 1 cut(s) 1031
DrdI GACNNNNNNGTC 1 cut(s) 2953
DseDI GACNNNNNNGTC 1 cut(s) 2953
Eam1104I CTCTTC 4 cut(s) 1311, 1512, 1524, 3214
EarI CTCTTC 4 cut(s) 1311, 1512, 1524, 3214
Ecl136II GAGCTC 1 cut(s) 364
Eco105I TACGTA 1 cut(s) 1244
Eco130I CCWWGG 3 cut(s) 667, 2008, 3136
Eco147I AGGCCT 1 cut(s) 1728
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 2 cut(s) 1919, 2576
Eco47I GGWCC 2 cut(s) 974, 1714
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 7 cut(s) 1548, 1604, 2685, 2744, 2963, 3227, 3419
Eco88I CYCGRG 3 cut(s) 702, 2060, 2141
Eco91I GGTNACC 1 cut(s) 2659
EcoICRI GAGCTC 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 2659
EcoRI GAATTC 2 cut(s) 117, 3164
EcoRII CCWGG 4 cut(s) 976, 1534, 3001, 3276
EcoT14I CCWWGG 3 cut(s) 667, 2008, 3136
EcoT22I ATGCAT 3 cut(s) 1274, 1479, 2845
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 3 cut(s) 667, 2008, 3136
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 1 cut(s) 1182
FauI CCCGC 3 cut(s) 608, 669, 2358
FauNDI CATATG 1 cut(s) 629
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 7 cut(s) 183, 341, 450, 745, 867, 2233, 2554
FokI GGATG 8 cut(s) 146, 266, 471, 863, 2343, 2484, 3158, 3496
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 7 cut(s) 183, 341, 450, 745, 867, 2233, 2554
FspBI CTAG 7 cut(s) 198, 788, 941, 954, 1388, 2514, 2795
GlaI GCGC 1 cut(s) 71
GluI GCNGC 7 cut(s) 183, 341, 450, 745, 867, 2233, 2554
GsaI CCCAGC 1 cut(s) 1504
HaeIII GGCC 5 cut(s) 294, 786, 1728, 2235, 3391
HapII CCGG 1 cut(s) 3392
HhaI GCGC 1 cut(s) 72
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 5 cut(s) 9, 574, 933, 1632, 2424
HindII GTYRAC 5 cut(s) 9, 574, 933, 1632, 2424
HindIII AAGCTT 2 cut(s) 2024, 2723
HpaII CCGG 1 cut(s) 3392
HphI GGTGA 5 cut(s) 115, 2627, 2671, 3284, 3415
Hpy166II GTNNAC 8 cut(s) 9, 558, 574, 635, 933, 1632, 1714, 2424
Hpy8I GTNNAC 8 cut(s) 9, 558, 574, 635, 933, 1632, 1714, 2424
Hpy99I CGWCG 1 cut(s) 24
HpyCH4III ACNGT 4 cut(s) 1470, 2378, 3086, 3353
HpyCH4IV ACGT 4 cut(s) 1243, 1281, 1543, 2164
HpyF10VI GCNNNNNNNGC 7 cut(s) 446, 678, 1483, 2722, 3110, 3552, 3561
HpySE526I ACGT 4 cut(s) 1243, 1281, 1543, 2164
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 70
LguI GCTCTTC 1 cut(s) 1524
LmnI GCTCC 2 cut(s) 400, 863
Lsp1109I GCAGC 3 cut(s) 169, 436, 731
LweI GCATC 7 cut(s) 28, 493, 1144, 1464, 2321, 2830, 2852
MaeI CTAG 7 cut(s) 198, 788, 941, 954, 1388, 2514, 2795
MaeII ACGT 4 cut(s) 1243, 1281, 1543, 2164
MaeIII GTNAC 4 cut(s) 1375, 2642, 2659, 3047
MbiI CCGCTC 1 cut(s) 2232
MfeI CAATTG 3 cut(s) 186, 2217, 2887
MflI RGATCY 7 cut(s) 200, 325, 663, 1784, 1966, 2314, 2489
MhlI GDGCHC 3 cut(s) 366, 1601, 3135
MlyI GAGTC 4 cut(s) 203, 1529, 2074, 3426
MmeI TCCRAC 6 cut(s) 1482, 2131, 2139, 2721, 3207, 3508
Mph1103I ATGCAT 3 cut(s) 1274, 1479, 2845
MroXI GAANNNNTTC 6 cut(s) 595, 807, 1077, 1509, 2948, 2985
MslI CAYNNNNRTG 5 cut(s) 590, 642, 1622, 2472, 3036
MspA1I CMGCKG 1 cut(s) 2227
MspCI CTTAAG 3 cut(s) 1780, 2735, 3052
MspI CCGG 1 cut(s) 3392
MspR9I CCNGG 5 cut(s) 978, 1536, 3003, 3278, 3393
MunI CAATTG 3 cut(s) 186, 2217, 2887
Mva1269I GAATGC 2 cut(s) 603, 2930
MvaI CCWGG 4 cut(s) 978, 1536, 3003, 3278
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 7 cut(s) 446, 678, 1483, 2722, 3110, 3552, 3561
NciI CCSGG 1 cut(s) 3393
NcoI CCATGG 2 cut(s) 667, 3136
NdeI CATATG 1 cut(s) 629
NlaIV GGNNCC 4 cut(s) 327, 344, 665, 865
NmeAIII GCCGAG 1 cut(s) 2322
NmuCI GTSAC 2 cut(s) 1375, 2659
NsiI ATGCAT 3 cut(s) 1274, 1479, 2845
NspI RCATGY 1 cut(s) 676
NspV TTCGAA 2 cut(s) 1288, 2934
OliI CACNNNNGTG 1 cut(s) 642
PaeI GCATGC 1 cut(s) 676
PaeR7I CTCGAG 2 cut(s) 702, 2060
PagI TCATGA 1 cut(s) 607
PceI AGGCCT 1 cut(s) 1728
PciSI GCTCTTC 1 cut(s) 1524
PcsI WCGNNNNNNNCGW 1 cut(s) 3040
PctI GAATGC 2 cut(s) 603, 2930
PdmI GAANNNNTTC 6 cut(s) 595, 807, 1077, 1509, 2948, 2985
PflMI CCANNNNNTGG 1 cut(s) 3142
PfoI TCCNGGA 1 cut(s) 3001
PkrI GCNGC 7 cut(s) 184, 342, 451, 746, 868, 2234, 2555
PleI GAGTC 4 cut(s) 202, 1528, 2073, 3425
PpsI GAGTC 4 cut(s) 202, 1528, 2073, 3425
Ppu21I YACGTR 1 cut(s) 1244
PshAI GACNNNNGTC 1 cut(s) 461
PshBI ATTAAT 1 cut(s) 2105
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 1 cut(s) 2164
Psp6I CCWGG 4 cut(s) 976, 1534, 3001, 3276
PspEI GGTNACC 1 cut(s) 2659
PspFI CCCAGC 1 cut(s) 1500
PspGI CCWGG 4 cut(s) 976, 1534, 3001, 3276
PspN4I GGNNCC 4 cut(s) 327, 344, 665, 865
PspPI GGNCC 4 cut(s) 292, 974, 1714, 3389
PspXI VCTCGAGB 1 cut(s) 702
PstI CTGCAG 1 cut(s) 2264
PsuI RGATCY 7 cut(s) 200, 325, 663, 1784, 1966, 2314, 2489
PvuII CAGCTG 1 cut(s) 2227
RsaI GTAC 4 cut(s) 2287, 2818, 3123, 3334
RsaNI GTAC 4 cut(s) 2286, 2817, 3122, 3333
RseI CAYNNNNRTG 5 cut(s) 590, 642, 1622, 2472, 3036
SacI GAGCTC 1 cut(s) 366
SapI GCTCTTC 1 cut(s) 1524
SatI GCNGC 7 cut(s) 183, 341, 450, 745, 867, 2233, 2554
Sau96I GGNCC 4 cut(s) 292, 974, 1714, 3389
SchI GAGTC 4 cut(s) 203, 1529, 2074, 3426
ScrFI CCNGG 5 cut(s) 978, 1536, 3003, 3278, 3393
SduI GDGCHC 3 cut(s) 366, 1601, 3135
SfaNI GCATC 7 cut(s) 28, 493, 1144, 1464, 2321, 2830, 2852
SfcI CTRYAG 1 cut(s) 2260
Sfr274I CTCGAG 2 cut(s) 702, 2060
SfuI TTCGAA 2 cut(s) 1288, 2934
SinI GGWCC 2 cut(s) 974, 1714
SlaI CTCGAG 2 cut(s) 702, 2060
SmiMI CAYNNNNRTG 5 cut(s) 590, 642, 1622, 2472, 3036
SmlI CTYRAG 8 cut(s) 146, 702, 1780, 1955, 2060, 2726, 2735, 3052
SmoI CTYRAG 8 cut(s) 146, 702, 1780, 1955, 2060, 2726, 2735, 3052
SnaBI TACGTA 1 cut(s) 1244
SphI GCATGC 1 cut(s) 676
SseBI AGGCCT 1 cut(s) 1728
SsiI CCGC 8 cut(s) 340, 601, 676, 867, 883, 2232, 2365, 2554
SspI AATATT 2 cut(s) 1329, 3013
SspMI CTAG 7 cut(s) 198, 788, 941, 954, 1388, 2514, 2795
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 1728
StyD4I CCNGG 5 cut(s) 976, 1534, 3001, 3276, 3391
StyI CCWWGG 3 cut(s) 667, 2008, 3136
TaaI ACNGT 4 cut(s) 1470, 2378, 3086, 3353
TaiI ACGT 4 cut(s) 1246, 1284, 1546, 2167
TaqII GACCGA 2 cut(s) 153, 753
TatI WGTACW 2 cut(s) 2816, 3121
TauI GCSGC 4 cut(s) 343, 869, 2235, 2556
TscAI CASTG 6 cut(s) 281, 2550, 2829, 3484, 3547, 3553
TseFI GTSAC 2 cut(s) 1375, 2659
TseI GCWGC 3 cut(s) 182, 449, 744
Tsp45I GTSAC 2 cut(s) 1375, 2659
TspGWI ACGGA 3 cut(s) 541, 1164, 3410
TspRI CASTG 6 cut(s) 281, 2550, 2829, 3484, 3547, 3553
Van91I CCANNNNNTGG 1 cut(s) 3142
Vha464I CTTAAG 3 cut(s) 1780, 2735, 3052
VpaK11BI GGWCC 2 cut(s) 974, 1714
VspI ATTAAT 1 cut(s) 2105
XbaI TCTAGA 2 cut(s) 197, 2513
XceI RCATGY 1 cut(s) 676
XcmI CCANNNNNNNNNTGG 3 cut(s) 823, 2006, 3134
XhoI CTCGAG 2 cut(s) 702, 2060
XmiI GTMKAC 1 cut(s) 557
XmnI GAANNNNTTC 6 cut(s) 595, 807, 1077, 1509, 2948, 2985
XspI CTAG 7 cut(s) 198, 788, 941, 954, 1388, 2514, 2795
Zsp2I ATGCAT 3 cut(s) 1274, 1479, 2845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.