RchiOBHm_Chr5g0055671

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000105
Physical Location & Seq
Forward (+)
58976289 .. 58984339
8051 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33263

Sequence Viewer

Length: 3498 bp
ATGCCCGTAGGAGTTTCGTCGGCCATCTGTATAAAGCATTGGAACGTAAAGCATTTCACGTTTTTGTGGATTCCGAGGGTCTGTCGAAAGGAGATTCCCCTGTTGATAGCGATTGAGGAGTCAAGAATTGCCATTGTCGTTCTTTCAGAGAATTTCACTTCGTCGTCGTGGTGCTTGGATGAAGTCGTGAAGATAATGCAATGCAAGAAAGAGATGGGACAAATAGTCTTTCCGGTCTTCTACAAAGTGGATCCTTCTGATGTGCGGCACCAAACGGGAAGTTTTCAGCTCCGTAGGAAATTGAAAGTGGAAGTAATGGAACACGAAGAAGTATATGGGGAGAATGAGGATAAGTTGAAGGAGTGGGCAGCCGCTTTGACAGAGCTGGCCAATCTTTCTGGCTGGCATTCCACGAATTACGGAGACGATGCAAGATTGATTGATGAGATTGTTGGTGATGCAATTAAAAAGTTTACTCATTTACTCTCTTCAAGTGTTGACAAGGGTTTCGTAGGAATGGACTCCCGCATTGGTGATTTGGTGCACAATTATATTTGTCCAGAGTTTGGTGGTGTTCGTATTATAGGGATCCATGGCATGCGAGGAATAGGCAAGACAACACTTGCTGTAGCTATTCATGATGAAATCCGTCAGGATTTTGACCGAAGCTGCTTTCTTTCTGGTGTTAGAGAAAGTGCCAAAAAGGATGGCCTAGTTTCTTTACAAAAAAAACTTCTTTTCAGTATCCTGGGGGCAGAAATTGACATACAGAATGAATATGCAGGAGCTACTATGATCAAAAGGCGGTTATGTAAAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCACCTCAATCAATTGGAGAAATTGGCTGGAAGTCATGACTGGTTTGGTCCAGGGAGTAGAATTGTTGTAACCACCCCAGATATTCATTTGCTAAAGACACATGATGTTGATGCTACATACAAGGCTACTGGGTTAAATGGTGGTGAAGCTATTCAACTATTGAGTTTAAAGGCTTTCAAGAAAAAAGTCCCACCAGAACATCATTTGGAGTTGTGCAACCTTATTTTAGGGTATGCTCAAGGGCTTCCATTGGCTGTTGTGGTTTTAGGTTCTTTTCTATCTGGTAGAAGCATTACGGAATGGGAAAGTGCAATAGATAGGCTAAATAATACACCACATCCAGAAATTATCGATGTGCTTCAGATTAGTTTTGATGGACTACAGGAAAAAGACAAAAATATATTCCTACATATAGCTTGCTTTTACAAGGGTTGGGATTTGGATCGTGTGACACAAATAATAGGCTATTGCGAGCTAGAACCTGACATTGGTTTAAGTGTTCTTGCTGATAGATCTCTCATAACCATCTCCAACAACAAACTGTCAATGCATGATTTGCTACAAGAACTGGGCTGGGAAATAGTTCGTCGACAGTCTCCTAGAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATAAAGAGATCCACAGTGTGTTAAAGAAGAATAAGGGAACAGATGCAATCCAAGCCATGGTAGTGGACTTGCCTAAGTTAGAAGTGGCTCATTGGAAACCAGAAGCCTTTTCAAAGTTGTCTCAACTCAGTCTTCTTCATATTCGTAACGTGGATCTTCCCAAAGGCCTCACTTTTCTTTCCAATTCCTTGAGACTTCTTGAATGGAGTGGATATCCCTTAAGATCCCTCCCACAAAATTTTGAACCGGATGAACTTATTGAACTTAACTTGTGCAACAGCAACATTGAATACCTCTGGAAAGGAGCAAAGAATTGTGACAAGTTAAAGTTCATCAAACTTTGCCATTCTCAAAAAATTGTCCAAACCCCAGACCTCACAGGCGTTCAAAATCTTGAGACTTTAGATCTTAAAGGATGTAAGACTTTGGTAAAACTTCATCAATCCCTCGGGCAACTCAAAAGGCTTACTGTATTGAATCTTAAAGATTGCAAGAGTCTCGAGAGTCTGCCAAGTAAGCTTGAAATGGAATCTCTTAAAACTTTAATTCTTTCTAACTGCTCAAAAGTTAAAAAGATTCCTGAGTTTGCTGGAAATATGAAGCGTTTATCGGAGCTTTATCTCGATGAGACTGCCATTGAAAAACTACCTGTGTCAATTGGATGCTTGAGTGGCCTTGCTTCGCTGAATCTGAGCAACTGCAAAAATCTTGTTTGCCTTCCAAGCACCTTTAGTAGATTGGAGTCTCTTAAAAAGCTTAATCTTTCTGGATGTTTGCAACTTGGTGAACAGGATAGTGCGATGGAGAATGGTGCAATTAGAACTGCTGTAAATCATCTTATGAAATATGGTTGTGAAGTTGTTGGGAAATATTTTTCATGGTCCTTGTCTTCTAGATTGGTGCAAGGAGCAGATATAGAACCAATGAGTATGCAGTTGCCTGTATCTGATCTGTGTTCTACGGAGAAAGATGCAAATACATCAATGAGTATCCAGTTGCTACTATCTCGTCTTTGTAATTTAACATATCTAAACATAAGCAACTGCAATCTTGGTGACGCAGCATTTGCCAACTTTGGCTGTTCTCCCTCCTTAGTGGCACTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGATCCTGTTTTAAGCTTCAGAACATTAACTTGGAGAATTGCAAGACACTTCAAGATTTGTCAGGCATTCCATCAAATAGTAAATTGGATGTGAGGGCAGATGGTTGTTCTTCACTGGAAATGCTGTTTGATGTATCCAATTTTAAGAGATTAGAGAAATCATATTTCAATTTCATCAATTGCTTCAAACTAAATGACAATCAGGGCTGCACTAACATAGCATTTGAAATGCTGAGGATATTCCTTAATCAGAGAATTTCTAGTGTGACGGAAACTTTTCAAATTGTAATTCCTGGAAGTAGAATTCCTAAGTGGTTCCATTATCGAAGTTTTGAGAGTTCATTAAGTGTAGACCTACTTCCAGGTTGGAATAAGAGTACGTTTTTGGGATTTTTTTTATGTGCTGTTTTTGTACTTGATGAGCACTTGGTTGATAAGCTTGAGTTCCATCAGTTCAAGACTTTTAAAGCTACTCATCATCTTGTATGTTGCCTGAAGCTCAATGGAAGAGAATTAGAAGTATACGGCAAACAGCCTGCATTTCGCTTTAGCGAACAATTTTGCCATGTTGAGTCAGATCACCTTTGGATATTCTATGTGTCCCGTGATAAATACTTTGGTACAGATTGGTGGCATAACAGTTGCAGTCAGCTTGAGTTCCTATTTAAAACCAGAGGGCCAGGTCTGAAGGTGAAGGAGTGCGGGGTGCGTTTGATATATGAGCACGATGTGCAAGAGTTGAACAAAACAATGACTCAATCAAGCAGTAGGATGTCTCCATATGAGGATATATTGATTGATTTTGAGAATCGAGTTGAAGGGGAAACCAGTGGTACTGGTAGCAGAACCTGCACCCTTGAAGAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1165

Amino Acids

131.53

Weight (kDa)

7.03

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 35 - 171 1.1e-33 TIR domain
NB-ARC PF00931 191 - 345 2.6e-16 NB-ARC domain
WHD_ROQ1 PF23282 412 - 482 3.2e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 664 - 753 3.1e-07 Leucine-rich repeat region
LRR_13 PF23286 671 - 758 2.5e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 973 - 1121 3.7e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 3485
AccB1I GGYRCC 1 cut(s) 267
AccB7I CCANNNNNTGG 2 cut(s) 566, 1540
AccI GTMKAC 3 cut(s) 1438, 2979, 3150
AciI CCGC 5 cut(s) 265, 372, 526, 805, 3330
AcoI YGGCCR 2 cut(s) 21, 387
AcsI RAATTY 4 cut(s) 151, 1720, 2883, 2931
AcuI CTGAAG 4 cut(s) 1193, 2630, 3143, 3335
AdeI CACNNNGTG 2 cut(s) 1501, 3358
AfaI GTAC 4 cut(s) 3007, 3042, 3250, 3463
AfiI CCNNNNNNNGG 3 cut(s) 566, 1337, 1540
AflII CTTAAG 1 cut(s) 1702
AhdI GACNNNNNGTC 1 cut(s) 224
AjnI CCWGG 6 cut(s) 747, 898, 1456, 2920, 2989, 3307
AjuI GAANNNNNNNTTGG 4 cut(s) 1320, 1352, 2642, 2674
Alw21I GWGCWC 3 cut(s) 546, 3054, 3354
Alw44I GTGCAC 1 cut(s) 542
AlwNI CAGNNNCTG 2 cut(s) 2634, 3477
Ama87I CYCGRG 2 cut(s) 1931, 1982
AoxI GGCC 6 cut(s) 21, 387, 709, 1648, 2155, 3305
ApaLI GTGCAC 1 cut(s) 542
ApeKI GCWGC 4 cut(s) 368, 669, 2543, 2835
ApoI RAATTY 4 cut(s) 151, 1720, 2883, 2931
ArsI GACNNNNNNTTYG 4 cut(s) 491, 523, 2531, 2563
Asp700I GAANNNNTTC 3 cut(s) 999, 1431, 2904
AspS9I GGNCC 3 cut(s) 896, 2364, 3305
AsuHPI GGTGA 8 cut(s) 467, 545, 842, 1004, 2279, 2549, 3200, 3331
AvaI CYCGRG 2 cut(s) 1931, 1982
AvaII GGWCC 2 cut(s) 896, 2364
BaeGI GKGCMC 1 cut(s) 546
BalI TGGCCA 1 cut(s) 389
BamHI GGATCC 2 cut(s) 250, 588
BanI GGYRCC 1 cut(s) 267
BarI GAAGNNNNNNTAC 2 cut(s) 2970, 3002
BbsI GAAGAC 3 cut(s) 229, 1607, 2364
Bbv12I GWGCWC 3 cut(s) 546, 3054, 3354
BbvCI CCTCAGC 1 cut(s) 2861
BbvI GCAGC 4 cut(s) 380, 656, 2555, 2822
BccI CCATC 9 cut(s) 32, 208, 701, 1217, 1382, 2278, 2707, 2723, 3084
BceAI ACGGC 1 cut(s) 3169
BcgI CGANNNNNNTGC 4 cut(s) 1963, 1997, 2096, 2130
BciT130I CCWGG 6 cut(s) 749, 900, 1458, 2922, 2991, 3309
BciVI GTATCC 3 cut(s) 755, 2483, 2773
BclI TGATCA 1 cut(s) 795
BfaI CTAG 5 cut(s) 713, 1325, 1449, 2376, 2889
BfmI CTRYAG 3 cut(s) 627, 1229, 3494
BfrI CTTAAG 1 cut(s) 1702
BfuAI ACCTGC 1 cut(s) 3485
BfuI GTATCC 3 cut(s) 755, 2483, 2773
BglII AGATCT 2 cut(s) 1361, 1888
BisI GCNGC 6 cut(s) 266, 369, 372, 670, 2544, 2836
BlsI GCNGC 6 cut(s) 267, 370, 373, 671, 2545, 2837
Bme1390I CCNGG 6 cut(s) 749, 900, 1458, 2922, 2991, 3309
Bme18I GGWCC 2 cut(s) 896, 2364
BmeRI GACNNNNNGTC 1 cut(s) 224
BmeT110I CYCGRG 2 cut(s) 1931, 1982
BmgT120I GGNCC 3 cut(s) 896, 2364, 3305
BmiI GGNNCC 4 cut(s) 252, 269, 590, 2945
BmrFI CCNGG 6 cut(s) 749, 900, 1458, 2922, 2991, 3309
BmrI ACTGGG 2 cut(s) 987, 1427
BmsI GCATC 7 cut(s) 418, 448, 949, 1516, 2135, 2443, 2634
BmuI ACTGGG 2 cut(s) 987, 1427
BpiI GAAGAC 3 cut(s) 229, 1607, 2364
Bpu10I CCTNAGC 1 cut(s) 2861
BpuEI CTTGAG 6 cut(s) 1071, 1693, 1898, 2170, 3089, 3302
Bsa29I ATCGAT 1 cut(s) 1200
BsaBI GATNNNNATC 2 cut(s) 846, 1290
BsaI GGTCTC 1 cut(s) 1485
BsaJI CCNNGG 6 cut(s) 74, 592, 748, 899, 1539, 1930
BsaWI WCCGGW 2 cut(s) 232, 1729
Bsc4I CCNNNNNNNGG 3 cut(s) 566, 1337, 1540
Bse1I ACTGG 7 cut(s) 893, 982, 1422, 2476, 2748, 3456, 3469
Bse3DI GCAATG 1 cut(s) 206
Bse8I GATNNNNATC 2 cut(s) 846, 1290
BseBI CCWGG 6 cut(s) 749, 900, 1458, 2922, 2991, 3309
BseCI ATCGAT 1 cut(s) 1200
BseDI CCNNGG 6 cut(s) 74, 592, 748, 899, 1539, 1930
BseGI GGATG 9 cut(s) 184, 712, 1186, 1738, 1904, 2150, 2258, 2722, 3405
BseJI GATNNNNATC 2 cut(s) 846, 1290
BseLI CCNNNNNNNGG 3 cut(s) 566, 1337, 1540
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 5 cut(s) 1624, 2055, 2165, 2582, 2852
BseNI ACTGG 7 cut(s) 893, 982, 1422, 2476, 2748, 3456, 3469
BseRI GAGGAG 1 cut(s) 131
BseSI GKGCMC 1 cut(s) 546
BseXI GCAGC 4 cut(s) 380, 656, 2555, 2822
BseYI CCCAGC 1 cut(s) 1422
BsgI GTGCAG 2 cut(s) 2821, 3463
BshFI GGCC 6 cut(s) 23, 389, 711, 1650, 2157, 3307
BshNI GGYRCC 1 cut(s) 267
BshVI ATCGAT 1 cut(s) 1200
BsiHKAI GWGCWC 3 cut(s) 546, 3054, 3354
BsiHKCI CYCGRG 2 cut(s) 1931, 1982
BsiSI CCGG 2 cut(s) 233, 1730
BslFI GGGAC 3 cut(s) 231, 1022, 3214
BslI CCNNNNNNNGG 3 cut(s) 566, 1337, 1540
BsmBI CGTCTC 1 cut(s) 417
BsmFI GGGAC 3 cut(s) 231, 1022, 3214
BsmI GAATGC 2 cut(s) 406, 2694
BsnI GGCC 6 cut(s) 23, 389, 711, 1650, 2157, 3307
Bso31I GGTCTC 1 cut(s) 1485
BsoBI CYCGRG 2 cut(s) 1931, 1982
Bsp1286I GDGCHC 3 cut(s) 546, 3054, 3354
Bsp19I CCATGG 2 cut(s) 592, 1539
BspACI CCGC 5 cut(s) 265, 372, 526, 805, 3330
BspANI GGCC 6 cut(s) 23, 389, 711, 1650, 2157, 3307
BspCNI CTCAG 5 cut(s) 1623, 2056, 2166, 2583, 2853
BspDI ATCGAT 1 cut(s) 1200
BspHI TCATGA 2 cut(s) 637, 883
BspLI GGNNCC 4 cut(s) 252, 269, 590, 2945
BspMI ACCTGC 1 cut(s) 3485
BspT107I GGYRCC 1 cut(s) 267
BspTI CTTAAG 1 cut(s) 1702
BspTNI GGTCTC 1 cut(s) 1485
BsrDI GCAATG 1 cut(s) 206
BsrI ACTGG 7 cut(s) 893, 982, 1422, 2476, 2748, 3456, 3469
BssECI CCNNGG 6 cut(s) 74, 592, 748, 899, 1539, 1930
BssNAI GTATAC 1 cut(s) 3151
BssT1I CCWWGG 2 cut(s) 592, 1539
Bst1107I GTATAC 1 cut(s) 3151
Bst2UI CCWGG 6 cut(s) 749, 900, 1458, 2922, 2991, 3309
Bst4CI ACNGT 5 cut(s) 1392, 1443, 1499, 1954, 3269
Bst6I CTCTTC 2 cut(s) 493, 3130
BstAFI CTTAAG 1 cut(s) 1702
BstAPI GCANNNNNTGC 4 cut(s) 1405, 2549, 3358, 3477
BstC8I GCNNGC 6 cut(s) 387, 404, 599, 1267, 1322, 3165
BstDEI CTNAG 8 cut(s) 1557, 1610, 2064, 2174, 2575, 2591, 2861, 2937
BstDSI CCRYGG 2 cut(s) 592, 1539
BstF5I GGATG 9 cut(s) 184, 712, 1186, 1738, 1904, 2150, 2258, 2722, 3405
BstMWI GCNNNNNNNGC 8 cut(s) 1405, 1535, 1999, 2154, 2205, 2549, 3358, 3477
BstNI CCWGG 6 cut(s) 749, 900, 1458, 2922, 2991, 3309
BstNSI RCATGY 1 cut(s) 601
BstSCI CCNGG 6 cut(s) 747, 898, 1456, 2920, 2989, 3307
BstSFI CTRYAG 3 cut(s) 627, 1229, 3494
BstSLI GKGCMC 1 cut(s) 546
BstV1I GCAGC 4 cut(s) 380, 656, 2555, 2822
BstV2I GAAGAC 3 cut(s) 229, 1607, 2364
BstX2I RGATCY 8 cut(s) 250, 588, 1361, 1491, 1636, 1706, 1888, 2630
BstXI CCANNNNNNTGG 1 cut(s) 1546
BstYI RGATCY 8 cut(s) 250, 588, 1361, 1491, 1636, 1706, 1888, 2630
BstZ17I GTATAC 1 cut(s) 3151
Bsu15I ATCGAT 1 cut(s) 1200
BsuI GTATCC 3 cut(s) 755, 2483, 2773
BsuRI GGCC 6 cut(s) 23, 389, 711, 1650, 2157, 3307
BsuTUI ATCGAT 1 cut(s) 1200
BtgI CCRYGG 2 cut(s) 592, 1539
BtgZI GCGATG 1 cut(s) 2297
BtsCI GGATG 9 cut(s) 184, 712, 1186, 1738, 1904, 2150, 2258, 2722, 3405
BtsIMutI CAGTG 3 cut(s) 1504, 2741, 3463
BveI ACCTGC 1 cut(s) 3485
Cac8I GCNNGC 6 cut(s) 387, 404, 599, 1267, 1322, 3165
CaiI CAGNNNCTG 2 cut(s) 2634, 3477
CciI TCATGA 2 cut(s) 637, 883
Cfr13I GGNCC 3 cut(s) 896, 2364, 3305
ClaI ATCGAT 1 cut(s) 1200
CseI GACGC 1 cut(s) 2549
Csp6I GTAC 4 cut(s) 3006, 3041, 3249, 3462
CviAII CATG 9 cut(s) 593, 598, 638, 884, 950, 1400, 1540, 2361, 3194
CviQI GTAC 4 cut(s) 3006, 3041, 3249, 3462
DdeI CTNAG 8 cut(s) 1557, 1610, 2064, 2174, 2575, 2591, 2861, 2937
DraI TTTAAA 3 cut(s) 1017, 3094, 3295
DraIII CACNNNGTG 2 cut(s) 1501, 3358
DriI GACNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 2 cut(s) 21, 387
Eam1104I CTCTTC 2 cut(s) 493, 3130
Eam1105I GACNNNNNGTC 1 cut(s) 224
EarI CTCTTC 2 cut(s) 493, 3130
Eco130I CCWWGG 2 cut(s) 592, 1539
Eco147I AGGCCT 1 cut(s) 1650
Eco31I GGTCTC 1 cut(s) 1485
Eco32I GATATC 1 cut(s) 1697
Eco47I GGWCC 2 cut(s) 896, 2364
Eco57I CTGAAG 4 cut(s) 1193, 2630, 3143, 3335
Eco88I CYCGRG 2 cut(s) 1931, 1982
EcoRI GAATTC 1 cut(s) 2931
EcoRII CCWGG 6 cut(s) 747, 898, 1456, 2920, 2989, 3307
EcoRV GATATC 1 cut(s) 1697
EcoT14I CCWWGG 2 cut(s) 592, 1539
EcoT22I ATGCAT 1 cut(s) 1401
ErhI CCWWGG 2 cut(s) 592, 1539
Esp3I CGTCTC 1 cut(s) 417
FaeI CATG 9 cut(s) 596, 601, 641, 887, 953, 1403, 1543, 2364, 3197
FaqI GGGAC 3 cut(s) 231, 1022, 3214
FatI CATG 9 cut(s) 592, 597, 637, 883, 949, 1399, 1539, 2360, 3193
FauI CCCGC 2 cut(s) 533, 3323
FauNDI CATATG 1 cut(s) 3409
FbaI TGATCA 1 cut(s) 795
FblI GTMKAC 3 cut(s) 1438, 2979, 3150
Fnu4HI GCNGC 6 cut(s) 266, 369, 372, 670, 2544, 2836
FokI GGATG 9 cut(s) 191, 719, 1173, 1745, 1911, 2157, 2265, 2729, 3412
Fsp4HI GCNGC 6 cut(s) 266, 369, 372, 670, 2544, 2836
FspBI CTAG 5 cut(s) 713, 1325, 1449, 2376, 2889
GluI GCNGC 6 cut(s) 266, 369, 372, 670, 2544, 2836
GsaI CCCAGC 1 cut(s) 1426
HaeIII GGCC 6 cut(s) 23, 389, 711, 1650, 2157, 3307
HapII CCGG 2 cut(s) 233, 1730
HgaI GACGC 1 cut(s) 2549
Hin1II CATG 9 cut(s) 596, 601, 641, 887, 953, 1403, 1543, 2364, 3197
HincII GTYRAC 2 cut(s) 499, 1439
HindII GTYRAC 2 cut(s) 499, 1439
HindIII AAGCTT 5 cut(s) 2000, 2237, 2612, 2642, 3065
HpaII CCGG 2 cut(s) 233, 1730
HphI GGTGA 8 cut(s) 467, 545, 842, 1004, 2279, 2549, 3200, 3331
Hpy166II GTNNAC 8 cut(s) 474, 499, 544, 1439, 1549, 2270, 2980, 3151
Hpy8I GTNNAC 8 cut(s) 474, 499, 544, 1439, 1549, 2270, 2980, 3151
Hpy99I CGWCG 4 cut(s) 22, 166, 169, 1440
HpyAV CCTTC 6 cut(s) 264, 352, 2210, 3310, 3316, 3440
HpyCH4III ACNGT 5 cut(s) 1392, 1443, 1499, 1954, 3269
HpyCH4IV ACGT 5 cut(s) 45, 59, 1465, 1632, 3008
HpyF10VI GCNNNNNNNGC 8 cut(s) 1405, 1535, 1999, 2154, 2205, 2549, 3358, 3477
HpyF3I CTNAG 8 cut(s) 1557, 1610, 2064, 2174, 2575, 2591, 2861, 2937
HpySE526I ACGT 5 cut(s) 45, 59, 1465, 1632, 3008
Hsp92II CATG 9 cut(s) 596, 601, 641, 887, 953, 1403, 1543, 2364, 3197
Ksp22I TGATCA 1 cut(s) 795
LmnI GCTCC 5 cut(s) 294, 785, 1787, 2095, 2390
Lsp1109I GCAGC 4 cut(s) 380, 656, 2555, 2822
LweI GCATC 7 cut(s) 418, 448, 949, 1516, 2135, 2443, 2634
MaeI CTAG 5 cut(s) 713, 1325, 1449, 2376, 2889
MaeII ACGT 5 cut(s) 45, 59, 1465, 1632, 3008
MaeIII GTNAC 6 cut(s) 916, 1297, 1628, 1799, 2537, 2893
MfeI CAATTG 3 cut(s) 860, 2139, 2806
MflI RGATCY 8 cut(s) 250, 588, 1361, 1491, 1636, 1706, 1888, 2630
MhlI GDGCHC 3 cut(s) 546, 3054, 3354
MlsI TGGCCA 1 cut(s) 389
MluNI TGGCCA 1 cut(s) 389
MlyI GAGTC 7 cut(s) 128, 515, 1987, 1996, 2234, 3209, 3376
MmeI TCCRAC 2 cut(s) 1404, 2975
Mox20I TGGCCA 1 cut(s) 389
Mph1103I ATGCAT 1 cut(s) 1401
MroXI GAANNNNTTC 3 cut(s) 999, 1431, 2904
MscI TGGCCA 1 cut(s) 389
MslI CAYNNNNRTG 1 cut(s) 1544
Msp20I TGGCCA 1 cut(s) 389
MspCI CTTAAG 1 cut(s) 1702
MspI CCGG 2 cut(s) 233, 1730
MspR9I CCNGG 6 cut(s) 749, 900, 1458, 2922, 2991, 3309
MunI CAATTG 3 cut(s) 860, 2139, 2806
Mva1269I GAATGC 2 cut(s) 406, 2694
MvaI CCWGG 6 cut(s) 749, 900, 1458, 2922, 2991, 3309
MwoI GCNNNNNNNGC 8 cut(s) 1405, 1535, 1999, 2154, 2205, 2549, 3358, 3477
NcoI CCATGG 2 cut(s) 592, 1539
NdeI CATATG 1 cut(s) 3409
NlaIII CATG 9 cut(s) 596, 601, 641, 887, 953, 1403, 1543, 2364, 3197
NlaIV GGNNCC 4 cut(s) 252, 269, 590, 2945
NmuCI GTSAC 4 cut(s) 1297, 1799, 2537, 2893
NsiI ATGCAT 1 cut(s) 1401
NspI RCATGY 1 cut(s) 601
PaeI GCATGC 1 cut(s) 601
PaeR7I CTCGAG 1 cut(s) 1982
PagI TCATGA 2 cut(s) 637, 883
PceI AGGCCT 1 cut(s) 1650
PctI GAATGC 2 cut(s) 406, 2694
PdmI GAANNNNTTC 3 cut(s) 999, 1431, 2904
PfeI GAWTC 7 cut(s) 70, 94, 1960, 2012, 2059, 2170, 3436
PflMI CCANNNNNTGG 2 cut(s) 566, 1540
PfoI TCCNGGA 1 cut(s) 2920
PkrI GCNGC 6 cut(s) 267, 370, 373, 671, 2545, 2837
PleI GAGTC 7 cut(s) 127, 515, 1986, 1995, 2233, 3208, 3376
PpsI GAGTC 7 cut(s) 127, 515, 1986, 1995, 2233, 3208, 3376
Psp6I CCWGG 6 cut(s) 747, 898, 1456, 2920, 2989, 3307
PspFI CCCAGC 1 cut(s) 1422
PspGI CCWGG 6 cut(s) 747, 898, 1456, 2920, 2989, 3307
PspN4I GGNNCC 4 cut(s) 252, 269, 590, 2945
PspPI GGNCC 3 cut(s) 896, 2364, 3305
PstNI CAGNNNCTG 2 cut(s) 2634, 3477
PsuI RGATCY 8 cut(s) 250, 588, 1361, 1491, 1636, 1706, 1888, 2630
RsaI GTAC 4 cut(s) 3007, 3042, 3250, 3463
RsaNI GTAC 4 cut(s) 3006, 3041, 3249, 3462
RseI CAYNNNNRTG 1 cut(s) 1544
SalI GTCGAC 1 cut(s) 1437
SatI GCNGC 6 cut(s) 266, 369, 372, 670, 2544, 2836
Sau96I GGNCC 3 cut(s) 896, 2364, 3305
SchI GAGTC 7 cut(s) 128, 515, 1987, 1996, 2234, 3209, 3376
ScrFI CCNGG 6 cut(s) 749, 900, 1458, 2922, 2991, 3309
SduI GDGCHC 3 cut(s) 546, 3054, 3354
SfaNI GCATC 7 cut(s) 418, 448, 949, 1516, 2135, 2443, 2634
SfcI CTRYAG 3 cut(s) 627, 1229, 3494
Sfr274I CTCGAG 1 cut(s) 1982
SinI GGWCC 2 cut(s) 896, 2364
SlaI CTCGAG 1 cut(s) 1982
SmiMI CAYNNNNRTG 1 cut(s) 1544
SmlI CTYRAG 8 cut(s) 1086, 1672, 1702, 1877, 1982, 2149, 3068, 3281
SmoI CTYRAG 8 cut(s) 1086, 1672, 1702, 1877, 1982, 2149, 3068, 3281
SphI GCATGC 1 cut(s) 601
SseBI AGGCCT 1 cut(s) 1650
SsiI CCGC 5 cut(s) 265, 372, 526, 805, 3330
SspI AATATT 1 cut(s) 2354
SspMI CTAG 5 cut(s) 713, 1325, 1449, 2376, 2889
StuI AGGCCT 1 cut(s) 1650
StyD4I CCNGG 6 cut(s) 747, 898, 1456, 2920, 2989, 3307
StyI CCWWGG 2 cut(s) 592, 1539
TaaI ACNGT 5 cut(s) 1392, 1443, 1499, 1954, 3269
TaiI ACGT 5 cut(s) 48, 62, 1468, 1635, 3011
TaqI TCGA 7 cut(s) 85, 1200, 1438, 1983, 2106, 2953, 3439
TaqII GACCGA 1 cut(s) 678
TatI WGTACW 1 cut(s) 3040
TauI GCSGC 2 cut(s) 268, 374
TfiI GAWTC 7 cut(s) 70, 94, 1960, 2012, 2059, 2170, 3436
TscAI CASTG 3 cut(s) 1504, 2748, 3463
TseFI GTSAC 4 cut(s) 1297, 1799, 2537, 2893
TseI GCWGC 4 cut(s) 368, 669, 2543, 2835
Tsp45I GTSAC 4 cut(s) 1297, 1799, 2537, 2893
TspGWI ACGGA 6 cut(s) 281, 435, 638, 1160, 2459, 2912
TspRI CASTG 3 cut(s) 1504, 2748, 3463
Van91I CCANNNNNTGG 2 cut(s) 566, 1540
Vha464I CTTAAG 1 cut(s) 1702
VneI GTGCAC 1 cut(s) 542
VpaK11BI GGWCC 2 cut(s) 896, 2364
XapI RAATTY 4 cut(s) 151, 1720, 2883, 2931
XbaI TCTAGA 1 cut(s) 2375
XceI RCATGY 1 cut(s) 601
XhoI CTCGAG 1 cut(s) 1982
XmiI GTMKAC 3 cut(s) 1438, 2979, 3150
XmnI GAANNNNTTC 3 cut(s) 999, 1431, 2904
XspI CTAG 5 cut(s) 713, 1325, 1449, 2376, 2889
Zsp2I ATGCAT 1 cut(s) 1401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.