MD10G1006800.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000105
Physical Location & Seq
Reverse (-)
1023305 .. 1027808
4504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1006800.v1.1.491

Sequence Viewer

Length: 3330 bp
ATGGGATCCACGGCGACTGCGCAAACAACTGATTACGATGTCTTCCTCAGTTTCAGTGGCGAAGACACTCGCAGGAATTTCGCAGACCAACTACGTGCTGCTCTGAGTAACCGAGGAGTCGTCGTATTTGGAGATGATGAACTTTATCGGGGAATTAAAAGTTCAAAAATTTCAATTGTCATTCTTTCAAGAAAGTATGCCAGGTCAACATGGTGCTTGGATGAGCTTCAGCAAATTATGGAATGCAAGGAAGTGGCTCGCCAGATAGTTTTGCCTGTCTTCTATGATGTGAGCCCATCCGAGGTACGAAAACAGACAGGCGATTTCGGTGACTTTTTTGCCGAGCATGAAGGACGTTTCAAAGACAATATCGACAAAGTGCGCGCCTGGAGGGATGCTCTTCGTCAATTAGCCAATCTGTCTGGATTTGACCTATACAAGGACGAGGATGGGTACGAGGCACAATTTATCCAACGAATTGTTCAATGGGTTTTGAATACATTGCAAACTGTGCCATCTTATCTTGAGGACATAGTGGGAATTGAGTCAAGAGTAGAGGAACTAAATAGGCTTTTGGACAACAAATCTAATGATGTTCGCTTCATAGGGATATGCGGAATGGGTGGGATAGGCAAGACAACTATCGCCAGAGTGATTTTTGACAACATTTCCAATGAATTTGAAATTTTCACCTGGATTACTTGTCGGGTAAGTCAAACACCACAGGGTGATCTGCAACTGCAAAAGAAACTTCTTTACGAGGCTTTGAGGCAAGACATAGATGTTCCAAATGTTAACGAGGGAGCCATAATGATAAAGAACATTCTATGTAAGAGAAAGGTTCTTCTCATTTTAGACAATGTTGTCAACTTAGGCCAACTGAAATCATTGGCTGGAAACAAAGAATGGTTTGGTTTGGGGAGCAGAGTCATCATTACGACTAGAGATGTGAATTTGCTAGGAGAGCATGGTGTAGATAGCAAATATAAAGTTGACTTATTAAGTGAGAATGAGGCTTTGCAGCTCCTCAAGAAGATCGCCTTTAAAAATGATGAATTTCGAGAAGATTATTTGGAGCTGTCTACACGGTTGGTTCATAAAGCTGGATACCTTCCTTTAGCACTCAAGACTGTGGGATCCTATTTGCTCCACAAAAGTCAGGAAGAGTGGCATAATGCATTGGAAAAACTGGAGTCTGTTCCTACTGGAAGAATTTCTGAAATGCTTGAAACAAGTTATCATGCACTGCCGGACGACGAGAAAAGAATATTTCTAGATATTGCTTGTTTCTTTCGAGGAATGGACAAGGAACGAGTGGTAGAGATACTAGATTATTTTGGATTTAATGCGATGGTTGGGATGGAAGTTCTCGTTGAGAGATCTCTCGTAACTATTTCAAATAACAAAGTGTTGATGCATGACTTGATACAAGAAATGGGACAACAAATTGTACGCAAAGACTCTGTTGTGGATCCGGGAGAGCAAAGCAGGTTGTGGCTTCATGAGGATGTCATTCATGTATTGAGGAACAATACAGGAACAAATGTGGTTGAAGGTATAGCCTTAGACTTGCCTAAATTAGAAGAGGCATGCCGGGACTTGGAGGCCTTCTCAACGATGCATAATCTCAGATTTCTCCGAATTCATAATTTGCGAATGGCCCAAGGGCCAAAAAAACTTTCTAATGCCTTAAAGTTTCTTGAATGGAGTGGGTATCCTTCAAAACTTCTCCCACCAGAATTTGAATTTGAGCAACTTTGTGAAGTTAACTTGTCCCATAGCAGCGTTGGACAACTTTGGAATAGAACAAAGTGCTTAGGCAATCTGAAATTCGTCAATGTTAGCTACTCCCAGAACCTGACCAGGACCCCGGACTTTACGGTTACTCCAAATCTTCATAGATTAATTCTTGAAGGCTGTACAAACTTGGTGATGATCCACCCATCCATTGCTGAGCTCAAAAGGCTTATTTTCTTAAATCTTAAAGACTGCAGAAGTCTTAAACGCCTTCCCAAATGTTTTGAAATGGAAAGTCTTCAAATTCTTATTCTTTCTGGTTGTTCAAAAATTAATAAAGTTCCAGAATTTGTGAGACCTATGGAAAACTTGTTGGAACTTTATTTAGATAAGACTGCTGTAGAACAATTACCTTCTTCAATCGAATGTCTGACTGGCCTTACTGTATTAAACCTGAGAGATTGCAGAAATCTCAGTTCCCTTGCAAGCAGTACTTGCCAGTTGCGTTCCCTCAAAAGTCTCAATCTCAGTGGATGCTCAAAGCTTGAAGAACTGCCAAAAAACTTGGGAAAGATGGTTTGTTTGAAGGAGCTTGATGTGGGCGGTACCTCTATTAATGATCTACCATCCTCCATTTCCCGCCTGGAAAATCTTGAATTGTTATCCCTTTGTGGTTGCAAATCGATGCGAAGAAAGAAGACAAGTCCAATGAGTTTGCTGTTGCCGACCACTGATTCTGGTTTGCTTTCTTTAACAGTACTGAATCTCAGTGACTGTAATCTTGAGGAGGTAACAATCCTGGAAAATCTTGGCTGCTTGTCCTCAATAGTATCACTTAATCTAAGTAAAAACAGTTTTGTTAGTCTTCCCAAGAGCATCACAGAGCTGTCTAAGCTTCAGATTTTGAATTTGGGTGGTTGTAAGAGTCTTAAAAAATTGCCGGACATTTCAGCAGAATTGAACTTCAGTGTTGAGGGAAAAGGCAGTTATTCACAGGAAAGGCTGTCATCATGTTTCAGTTTTATTAATTGCTTTGAAGTGGTTGACGACCAAGGCTGCAACAAAGTAGCATTTGCAGCGTTAAGGAGATTCCTTGAGGGAATCCCTTACATAGGGAATAAATTTGAAGCTATATATCCAGGCAGTGAAATTTCTGAGTGGTTTAGTGATCAAAGTGAGAGGCCTGTGGTAAGCATGGAGCTCCCTCGACACTGGTATAAGAACGAGTGGATTGGATATGCATTGTGTGCTGTTTTCACTGTCCATCGACAACTCCCACCTAATCTGCTTGGTAAATGGAACTACGGAACCCATACCACTGCACATGGTCTTCGTTGCGAAGTGAAGCCTGGGAATTTGGGTGTAGGTGGCTGGTGTCCTTCCTTCGCCTGCAGTGAAGAATTAGGGCAAATTGAGAAAGAGCACCTTTGGATATCCTTTGTATCTGGTGAACACTTTGGTACAACATGGCAAGACAGTTGTCATCACCTTGAGTTCACATTTAAAACCTTGGGCTCTGGTTTGGAGGTGAAGAGGTGCGGAGTCCGTCTCATCTACGAGAGAGATTTGGTTGGATCAAAGCAACCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1110

Amino Acids

125.42

Weight (kDa)

6.08

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 173 7.6e-40 TIR domain
TIR_2 PF13676 14 - 113 1e-12 TIR domain
NB-ARC PF00931 182 - 351 2e-26 NB-ARC domain
WHD_ROQ1 PF23282 419 - 488 1.6e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 677 - 763 5e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_R13L1-DRL21 PF25019 690 - 758 3.1e-06 R13L1/DRL21 LRRs
LRR_14 PF23598 720 - 807 4.5e-11 Leucine-rich repeat region
LRR_13 PF23286 762 - 895 8.7e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 829 - 896 4e-07 Leucine-rich repeat region
C-JID PF20160 959 - 1101 3.6e-14 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 863
Acc16I TGCGCA 1 cut(s) 21
Acc36I ACCTGC 1 cut(s) 1479
Acc65I GGTACC 1 cut(s) 2342
AccB1I GGYRCC 1 cut(s) 2342
AccI GTMKAC 1 cut(s) 1082
AccII CGCG 1 cut(s) 384
AciI CCGC 4 cut(s) 615, 2340, 2377, 3276
AclWI GGATC 7 cut(s) 13, 1131, 1144, 1466, 1479, 1930, 3319
AcuI CTGAAG 3 cut(s) 212, 2618, 2686
AdeI CACNNNGTG 1 cut(s) 728
AfaI GTAC 8 cut(s) 306, 455, 1452, 1921, 2230, 2344, 2496, 3199
AfiI CCNNNNNNNGG 4 cut(s) 301, 439, 1600, 2849
AjnI CCWGG 8 cut(s) 200, 386, 692, 1862, 2379, 2535, 2875, 3085
AjuI GAANNNNNNNTTGG 4 cut(s) 465, 497, 1663, 1695
Alw21I GWGCWC 3 cut(s) 1959, 2940, 3162
Alw26I GTCTC 3 cut(s) 2086, 2261, 3290
AlwI GGATC 7 cut(s) 13, 1131, 1144, 1466, 1479, 1930, 3319
AlwNI CAGNNNCTG 2 cut(s) 1858, 2511
AoxI GGCC 6 cut(s) 874, 1605, 1659, 1667, 2173, 2918
ApeKI GCWGC 6 cut(s) 98, 1021, 1782, 2550, 2793, 2813
AseI ATTAAT 4 cut(s) 1904, 2070, 2352, 2763
Asp700I GAANNNNTTC 2 cut(s) 1213, 2034
Asp718I GGTACC 1 cut(s) 2342
AspLEI GCGC 3 cut(s) 22, 384, 386
AspS9I GGNCC 3 cut(s) 1660, 1667, 1866
AsuC2I CCSGG 3 cut(s) 1476, 1595, 1871
AsuHPI GGTGA 7 cut(s) 341, 682, 740, 1942, 3197, 3215, 3277
AvaII GGWCC 1 cut(s) 1866
BamHI GGATCC 3 cut(s) 5, 1136, 1471
BanI GGYRCC 1 cut(s) 2342
BanII GRGCYC 4 cut(s) 296, 1959, 2940, 3254
BbsI GAAGAC 7 cut(s) 34, 69, 271, 2027, 2441, 2594, 3059
Bbv12I GWGCWC 3 cut(s) 1959, 2940, 3162
BbvI GCAGC 6 cut(s) 85, 1033, 1794, 2537, 2780, 2825
BccI CCATC 9 cut(s) 304, 443, 523, 1345, 1354, 1951, 2305, 2371, 3009
BceAI ACGGC 1 cut(s) 27
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 8 cut(s) 202, 388, 694, 1864, 2381, 2537, 2877, 3087
BciVI GTATCC 2 cut(s) 1100, 1725
BclI TGATCA 1 cut(s) 2905
BcnI CCSGG 3 cut(s) 1476, 1595, 1871
BcoDI GTCTC 3 cut(s) 2086, 2261, 3290
BfaI CTAG 4 cut(s) 942, 959, 1274, 1328
BfmI CTRYAG 3 cut(s) 1990, 2136, 3127
BfuAI ACCTGC 1 cut(s) 1479
BfuI GTATCC 2 cut(s) 1100, 1725
BglII AGATCT 1 cut(s) 1379
BisI GCNGC 6 cut(s) 99, 1022, 1783, 2551, 2794, 2814
BlpI GCTNAGC 1 cut(s) 1953
BlsI GCNGC 6 cut(s) 100, 1023, 1784, 2552, 2795, 2815
BmcAI AGTACT 2 cut(s) 2230, 2496
Bme18I GGWCC 1 cut(s) 1866
BmgT120I GGNCC 3 cut(s) 1660, 1667, 1866
BmiI GGNNCC 7 cut(s) 7, 805, 1138, 1473, 1868, 2344, 3046
BmsI GCATC 6 cut(s) 385, 1404, 1608, 2261, 2412, 2622
BoxI GACNNNNGTC 1 cut(s) 3216
BpiI GAAGAC 7 cut(s) 34, 69, 271, 2027, 2441, 2594, 3059
BplI GAGNNNNNCTC 6 cut(s) 382, 414, 1595, 1627, 3270, 3302
BpmI CTGGAG 2 cut(s) 409, 1211
Bpu10I CCTNAGC 1 cut(s) 1816
Bpu1102I GCTNAGC 1 cut(s) 1953
BpuEI CTTGAG 6 cut(s) 545, 1013, 1109, 2540, 2852, 3248
BpuMI CCSGG 3 cut(s) 1476, 1595, 1871
Bsa29I ATCGAT 1 cut(s) 2420
BsaAI YACGTR 1 cut(s) 95
BsaI GGTCTC 1 cut(s) 2086
BsaJI CCNNGG 8 cut(s) 9, 112, 300, 1663, 1869, 2788, 3086, 3246
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 1870, 1900
Bsc4I CCNNNNNNNGG 4 cut(s) 301, 439, 1600, 2849
Bse1I ACTGG 5 cut(s) 1194, 1210, 2176, 2236, 2954
Bse3DI GCAATG 2 cut(s) 500, 1947
BseBI CCWGG 8 cut(s) 202, 388, 694, 1864, 2381, 2537, 2877, 3087
BseCI ATCGAT 1 cut(s) 2420
BseDI CCNNGG 8 cut(s) 9, 112, 300, 1663, 1869, 2788, 3086, 3246
BseGI GGATG 9 cut(s) 226, 296, 400, 454, 1365, 1513, 1943, 2276, 2363
BseLI CCNNNNNNNGG 4 cut(s) 301, 439, 1600, 2849
BseMI GCAATG 2 cut(s) 500, 1947
BseMII CTCAG 9 cut(s) 61, 95, 1642, 1944, 2183, 2224, 2278, 2518, 2883
BseNI ACTGG 5 cut(s) 1194, 1210, 2176, 2236, 2954
BsePI GCGCGC 1 cut(s) 382
BseRI GAGGAG 3 cut(s) 129, 1016, 2537
BseXI GCAGC 6 cut(s) 85, 1033, 1794, 2537, 2780, 2825
BsgI GTGCAG 1 cut(s) 3042
Bsh1236I CGCG 1 cut(s) 384
BshFI GGCC 6 cut(s) 876, 1607, 1661, 1669, 2175, 2920
BshNI GGYRCC 1 cut(s) 2342
BshVI ATCGAT 1 cut(s) 2420
BsiHKAI GWGCWC 3 cut(s) 1959, 2940, 3162
BsiSI CCGG 5 cut(s) 1250, 1475, 1594, 1871, 2678
BslFI GGGAC 3 cut(s) 1452, 1610, 1759
BslI CCNNNNNNNGG 4 cut(s) 301, 439, 1600, 2849
BsmAI GTCTC 3 cut(s) 2086, 2261, 3290
BsmBI CGTCTC 1 cut(s) 3290
BsmFI GGGAC 3 cut(s) 1452, 1610, 1759
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 6 cut(s) 876, 1607, 1661, 1669, 2175, 2920
Bso31I GGTCTC 1 cut(s) 2086
Bsp1286I GDGCHC 5 cut(s) 296, 1959, 2940, 3162, 3254
Bsp1407I TGTACA 1 cut(s) 1919
Bsp1720I GCTNAGC 1 cut(s) 1953
BspACI CCGC 4 cut(s) 615, 2340, 2377, 3276
BspANI GGCC 6 cut(s) 876, 1607, 1661, 1669, 2175, 2920
BspCNI CTCAG 9 cut(s) 60, 96, 1641, 1945, 2184, 2223, 2277, 2517, 2884
BspDI ATCGAT 1 cut(s) 2420
BspFNI CGCG 1 cut(s) 384
BspHI TCATGA 1 cut(s) 1501
BspLI GGNNCC 7 cut(s) 7, 805, 1138, 1473, 1868, 2344, 3046
BspMAI CTGCAG 2 cut(s) 1994, 3131
BspMI ACCTGC 1 cut(s) 1479
BspPI GGATC 7 cut(s) 13, 1131, 1144, 1466, 1479, 1930, 3319
BspQI GCTCTTC 1 cut(s) 405
BspT107I GGYRCC 1 cut(s) 2342
BspTNI GGTCTC 1 cut(s) 2086
BsrDI GCAATG 2 cut(s) 500, 1947
BsrGI TGTACA 1 cut(s) 1919
BsrI ACTGG 5 cut(s) 1194, 1210, 2176, 2236, 2954
BssECI CCNNGG 8 cut(s) 9, 112, 300, 1663, 1869, 2788, 3086, 3246
BssHII GCGCGC 1 cut(s) 382
BssT1I CCWWGG 3 cut(s) 1663, 2788, 3246
Bst2UI CCWGG 8 cut(s) 202, 388, 694, 1864, 2381, 2537, 2877, 3087
Bst6I CTCTTC 4 cut(s) 405, 1158, 1578, 3263
BstAPI GCANNNNNTGC 3 cut(s) 511, 2232, 2984
BstAUI TGTACA 1 cut(s) 1919
BstBAI YACGTR 1 cut(s) 95
BstC8I GCNNGC 5 cut(s) 259, 384, 1591, 2224, 3127
BstDSI CCRYGG 1 cut(s) 9
BstENI CCTNNNNNAGG 2 cut(s) 437, 2847
BstF5I GGATG 9 cut(s) 226, 296, 400, 454, 1365, 1513, 1943, 2276, 2363
BstFNI CGCG 1 cut(s) 384
BstHHI GCGC 3 cut(s) 22, 384, 386
BstMAI GTCTC 3 cut(s) 2086, 2261, 3290
BstMWI GCNNNNNNNGC 7 cut(s) 511, 964, 1963, 2232, 2629, 2813, 2984
BstNI CCWGG 8 cut(s) 202, 388, 694, 1864, 2381, 2537, 2877, 3087
BstNSI RCATGY 1 cut(s) 1593
BstPAI GACNNNNGTC 1 cut(s) 3216
BstSFI CTRYAG 3 cut(s) 1990, 2136, 3127
BstUI CGCG 1 cut(s) 384
BstV1I GCAGC 6 cut(s) 85, 1033, 1794, 2537, 2780, 2825
BstV2I GAAGAC 7 cut(s) 34, 69, 271, 2027, 2441, 2594, 3059
BstX2I RGATCY 4 cut(s) 5, 1136, 1379, 1471
BstYI RGATCY 4 cut(s) 5, 1136, 1379, 1471
Bsu15I ATCGAT 1 cut(s) 2420
BsuI GTATCC 2 cut(s) 1100, 1725
BsuRI GGCC 6 cut(s) 876, 1607, 1661, 1669, 2175, 2920
BsuTUI ATCGAT 1 cut(s) 2420
BtgI CCRYGG 1 cut(s) 9
BtgZI GCGATG 1 cut(s) 1364
BtsCI GGATG 9 cut(s) 226, 296, 400, 454, 1365, 1513, 1943, 2276, 2363
BtsI GCAGTG 4 cut(s) 1244, 2887, 3054, 3136
BveI ACCTGC 1 cut(s) 1479
Cac8I GCNNGC 5 cut(s) 259, 384, 1591, 2224, 3127
CaiI CAGNNNCTG 2 cut(s) 1858, 2511
CciI TCATGA 1 cut(s) 1501
CfoI GCGC 3 cut(s) 22, 384, 386
Cfr13I GGNCC 3 cut(s) 1660, 1667, 1866
ClaI ATCGAT 1 cut(s) 2420
Csp6I GTAC 8 cut(s) 305, 454, 1451, 1920, 2229, 2343, 2495, 3198
CspCI CAANNNNNGTGG 2 cut(s) 2248, 2283
CviQI GTAC 8 cut(s) 305, 454, 1451, 1920, 2229, 2343, 2495, 3198
DraI TTTAAA 2 cut(s) 1045, 3241
DraIII CACNNNGTG 1 cut(s) 728
DrdI GACNNNNNNGTC 1 cut(s) 863
DseDI GACNNNNNNGTC 1 cut(s) 863
Eam1104I CTCTTC 4 cut(s) 405, 1158, 1578, 3263
EarI CTCTTC 4 cut(s) 405, 1158, 1578, 3263
Ecl136II GAGCTC 2 cut(s) 1957, 2938
Eco130I CCWWGG 3 cut(s) 1663, 2788, 3246
Eco147I AGGCCT 2 cut(s) 1607, 2920
Eco24I GRGCYC 4 cut(s) 296, 1959, 2940, 3254
Eco31I GGTCTC 1 cut(s) 2086
Eco32I GATATC 1 cut(s) 3171
Eco47I GGWCC 1 cut(s) 1866
Eco53kI GAGCTC 2 cut(s) 1957, 2938
Eco57I CTGAAG 3 cut(s) 212, 2618, 2686
EcoICRI GAGCTC 2 cut(s) 1957, 2938
EcoNI CCTNNNNNAGG 2 cut(s) 437, 2847
EcoO109I RGGNCCY 1 cut(s) 1866
EcoRI GAATTC 1 cut(s) 1641
EcoRII CCWGG 8 cut(s) 200, 386, 692, 1862, 2379, 2535, 2875, 3085
EcoRV GATATC 1 cut(s) 3171
EcoT14I CCWWGG 3 cut(s) 1663, 2788, 3246
EcoT22I ATGCAT 4 cut(s) 1180, 1419, 1623, 2980
EcoT38I GRGCYC 4 cut(s) 296, 1959, 2940, 3254
ErhI CCWWGG 3 cut(s) 1663, 2788, 3246
Esp3I CGTCTC 1 cut(s) 3290
FalI AAGNNNNNCTT 6 cut(s) 1025, 1057, 2215, 2247, 3147, 3179
FaqI GGGAC 3 cut(s) 1452, 1610, 1759
FauI CCCGC 1 cut(s) 2384
FbaI TGATCA 1 cut(s) 2905
FblI GTMKAC 1 cut(s) 1082
Fnu4HI GCNGC 6 cut(s) 99, 1022, 1783, 2551, 2794, 2814
FokI GGATG 9 cut(s) 233, 283, 407, 461, 1372, 1520, 1930, 2283, 2350
FriOI GRGCYC 4 cut(s) 296, 1959, 2940, 3254
Fsp4HI GCNGC 6 cut(s) 99, 1022, 1783, 2551, 2794, 2814
FspBI CTAG 4 cut(s) 942, 959, 1274, 1328
FspI TGCGCA 1 cut(s) 21
GlaI GCGC 3 cut(s) 21, 383, 385
GluI GCNGC 6 cut(s) 99, 1022, 1783, 2551, 2794, 2814
GsuI CTGGAG 2 cut(s) 409, 1211
HaeIII GGCC 6 cut(s) 876, 1607, 1661, 1669, 2175, 2920
HapII CCGG 5 cut(s) 1250, 1475, 1594, 1871, 2678
HhaI GCGC 3 cut(s) 22, 384, 386
Hin6I GCGC 3 cut(s) 20, 382, 384
HinP1I GCGC 3 cut(s) 20, 382, 384
HincII GTYRAC 6 cut(s) 207, 796, 868, 994, 1768, 2782
HindII GTYRAC 6 cut(s) 207, 796, 868, 994, 1768, 2782
HindIII AAGCTT 2 cut(s) 2279, 2630
HpaI GTTAAC 2 cut(s) 796, 1768
HpaII CCGG 5 cut(s) 1250, 1475, 1594, 1871, 2678
HphI GGTGA 7 cut(s) 341, 682, 740, 1942, 3197, 3215, 3277
Hpy166II GTNNAC 9 cut(s) 207, 796, 868, 994, 1083, 1768, 2782, 3188, 3234
Hpy188I TCNGA 9 cut(s) 105, 301, 1219, 1631, 1640, 1827, 2169, 2637, 2893
Hpy8I GTNNAC 9 cut(s) 207, 796, 868, 994, 1083, 1768, 2782, 3188, 3234
Hpy99I CGWCG 2 cut(s) 125, 1259
HpyCH4IV ACGT 2 cut(s) 94, 355
HpyF10VI GCNNNNNNNGC 7 cut(s) 511, 964, 1963, 2232, 2629, 2813, 2984
HpySE526I ACGT 2 cut(s) 94, 355
HspAI GCGC 3 cut(s) 20, 382, 384
KpnI GGTACC 1 cut(s) 2346
Ksp22I TGATCA 1 cut(s) 2905
KspAI GTTAAC 2 cut(s) 796, 1768
Kzo9I GATC 2 cut(s) 5, 730
LguI GCTCTTC 1 cut(s) 405
LmnI GCTCC 8 cut(s) 803, 921, 1029, 1075, 1152, 2326, 2935, 2943
Lsp1109I GCAGC 6 cut(s) 85, 1033, 1794, 2537, 2780, 2825
LweI GCATC 6 cut(s) 385, 1404, 1608, 2261, 2412, 2622
MaeI CTAG 4 cut(s) 942, 959, 1274, 1328
MaeII ACGT 2 cut(s) 94, 355
MaeIII GTNAC 6 cut(s) 107, 329, 1387, 1882, 2507, 2527
MfeI CAATTG 1 cut(s) 174
MflI RGATCY 4 cut(s) 5, 1136, 1379, 1471
MhlI GDGCHC 5 cut(s) 296, 1959, 2940, 3162, 3254
MlyI GAGTC 7 cut(s) 126, 554, 936, 1202, 1454, 2671, 3288
MmeI TCCRAC 4 cut(s) 496, 1768, 2091, 3289
Mph1103I ATGCAT 4 cut(s) 1180, 1419, 1623, 2980
MroXI GAANNNNTTC 2 cut(s) 1213, 2034
MslI CAYNNNNRTG 1 cut(s) 1506
MspI CCGG 5 cut(s) 1250, 1475, 1594, 1871, 2678
MunI CAATTG 1 cut(s) 174
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 8 cut(s) 202, 388, 694, 1864, 2381, 2537, 2877, 3087
MvnI CGCG 1 cut(s) 384
MwoI GCNNNNNNNGC 7 cut(s) 511, 964, 1963, 2232, 2629, 2813, 2984
NciI CCSGG 3 cut(s) 1476, 1595, 1871
NlaIV GGNNCC 7 cut(s) 7, 805, 1138, 1473, 1868, 2344, 3046
NmeAIII GCCGAG 1 cut(s) 367
NmuCI GTSAC 2 cut(s) 329, 2507
NsbI TGCGCA 1 cut(s) 21
NsiI ATGCAT 4 cut(s) 1180, 1419, 1623, 2980
NspI RCATGY 1 cut(s) 1593
PaeI GCATGC 1 cut(s) 1593
PagI TCATGA 1 cut(s) 1501
PauI GCGCGC 1 cut(s) 382
PceI AGGCCT 2 cut(s) 1607, 2920
PciSI GCTCTTC 1 cut(s) 405
PctI GAATGC 1 cut(s) 248
PdmI GAANNNNTTC 2 cut(s) 1213, 2034
PfeI GAWTC 4 cut(s) 2471, 2500, 2826, 2838
PfoI TCCNGGA 2 cut(s) 1474, 2535
PkrI GCNGC 6 cut(s) 100, 1023, 1784, 2552, 2795, 2815
PleI GAGTC 7 cut(s) 125, 553, 935, 1201, 1454, 2670, 3287
PpsI GAGTC 7 cut(s) 125, 553, 935, 1201, 1454, 2670, 3287
Ppu21I YACGTR 1 cut(s) 95
PpuMI RGGWCCY 1 cut(s) 1866
PshAI GACNNNNGTC 1 cut(s) 3216
PshBI ATTAAT 4 cut(s) 1904, 2070, 2352, 2763
Psp124BI GAGCTC 2 cut(s) 1959, 2940
Psp5II RGGWCCY 1 cut(s) 1866
Psp6I CCWGG 8 cut(s) 200, 386, 692, 1862, 2379, 2535, 2875, 3085
PspGI CCWGG 8 cut(s) 200, 386, 692, 1862, 2379, 2535, 2875, 3085
PspN4I GGNNCC 7 cut(s) 7, 805, 1138, 1473, 1868, 2344, 3046
PspPI GGNCC 3 cut(s) 1660, 1667, 1866
PspPPI RGGWCCY 1 cut(s) 1866
PstI CTGCAG 2 cut(s) 1994, 3131
PstNI CAGNNNCTG 2 cut(s) 1858, 2511
PsuI RGATCY 4 cut(s) 5, 1136, 1379, 1471
PteI GCGCGC 1 cut(s) 382
RsaI GTAC 8 cut(s) 306, 455, 1452, 1921, 2230, 2344, 2496, 3199
RsaNI GTAC 8 cut(s) 305, 454, 1451, 1920, 2229, 2343, 2495, 3198
RseI CAYNNNNRTG 1 cut(s) 1506
SacI GAGCTC 2 cut(s) 1959, 2940
SapI GCTCTTC 1 cut(s) 405
SatI GCNGC 6 cut(s) 99, 1022, 1783, 2551, 2794, 2814
Sau96I GGNCC 3 cut(s) 1660, 1667, 1866
ScaI AGTACT 2 cut(s) 2230, 2496
SchI GAGTC 7 cut(s) 126, 554, 936, 1202, 1454, 2671, 3288
SduI GDGCHC 5 cut(s) 296, 1959, 2940, 3162, 3254
SfaNI GCATC 6 cut(s) 385, 1404, 1608, 2261, 2412, 2622
SfcI CTRYAG 3 cut(s) 1990, 2136, 3127
SinI GGWCC 1 cut(s) 1866
SmiMI CAYNNNNRTG 1 cut(s) 1506
SmlI CTYRAG 6 cut(s) 524, 1028, 1124, 2519, 2831, 3227
SmoI CTYRAG 6 cut(s) 524, 1028, 1124, 2519, 2831, 3227
SphI GCATGC 1 cut(s) 1593
SseBI AGGCCT 2 cut(s) 1607, 2920
SsiI CCGC 4 cut(s) 615, 2340, 2377, 3276
SspI AATATT 1 cut(s) 1269
SspMI CTAG 4 cut(s) 942, 959, 1274, 1328
SstI GAGCTC 2 cut(s) 1959, 2940
StuI AGGCCT 2 cut(s) 1607, 2920
StyI CCWWGG 3 cut(s) 1663, 2788, 3246
TaiI ACGT 2 cut(s) 97, 358
TaqI TCGA 7 cut(s) 372, 1060, 1294, 2160, 2420, 2944, 3004
TatI WGTACW 3 cut(s) 1919, 2228, 2494
TfiI GAWTC 4 cut(s) 2471, 2500, 2826, 2838
TseFI GTSAC 2 cut(s) 329, 2507
TseI GCWGC 6 cut(s) 98, 1021, 1782, 2550, 2793, 2813
Tsp45I GTSAC 2 cut(s) 329, 2507
TspGWI ACGGA 2 cut(s) 3057, 3272
VpaK11BI GGWCC 1 cut(s) 1866
VspI ATTAAT 4 cut(s) 1904, 2070, 2352, 2763
XagI CCTNNNNNAGG 2 cut(s) 437, 2847
XbaI TCTAGA 1 cut(s) 1273
XceI RCATGY 1 cut(s) 1593
XcmI CCANNNNNNNNNTGG 2 cut(s) 1784, 2377
XmiI GTMKAC 1 cut(s) 1082
XmnI GAANNNNTTC 2 cut(s) 1213, 2034
XspI CTAG 4 cut(s) 942, 959, 1274, 1328
ZrmI AGTACT 2 cut(s) 2230, 2496
Zsp2I ATGCAT 4 cut(s) 1180, 1419, 1623, 2980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.