Rmu_sc0004439.1_g000008

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004439.1
OG0000105
Physical Location & Seq
Forward (+)
43695 .. 50069
6375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004439.1_g000008.1.cds

Sequence Viewer

Length: 5001 bp
atggcaaagatggacctcgaactcgacctcgactttgcgaagctatattcatgcctaagacctaccgatgcggcaaacatggacctcgacctcgacctcgacgacgacgacgacgacgacatggtaggatactgttttgatgagcacattggctattttttcggtggtgaccgtgacgacgacgacgacgacacgatggcaagataccggactgttgcctgcgcctacgcggatgagcactatcatttccgtcgtgattgggaccttcacgatgactacgacatagacaacaaatatgaggatattagtagcagtgagtcccctgggtcgtcaagtttttctccccgatatggccaatcgtacagatacgatgttttcctaagtttcagaaacgaacacaccgtctcgagttttgtgagccatctatatgcagcattagatcggagtggaatggtctcctttaatgatgaccaaaggcgtgacagaggggaattccctagttctgatccagaactagtttcaaaagctatcaaggactcaaaagttgccattgtcattttttcacgagactatgttacggcaacatggtgcgtggatgagctagagaagattctagtgtgcagagatatggggcgactagtactgcccatcttttacaatatcccggcttctgttgtacggggcatctacaatgggaaatatttgaaaaggaccgatgctttggtaaggttggccaacctttccggctacgttcttcaagatggaatggacgaggcaaatgtcatcaaaagtatcatttctgcaacagtaaaacatgtatctcataaaggttcaaatgttgacaagagcttcctaggcacagatcaccaatcgtacagatatcgccaatcgtacagatacgatgttttcctaagtttcagaaaggaacacactgtctcgagttttgtgagccatctatatgcagcattagatcggagtggaatagtctcctttaaagatgaccaaaggcgtgacagaggggaattccctagttctgatccacaactagtttcaaaagctatcgaggactcaaaagttgcccttgtcattttttcacgaaactatggtacggcaacatggtgcgtggatgagctagtgaagattctacagtgcagacgagatatggggcaactagtactgcccatcttttacaatatcccgacttctgttgtacggggcatctatatgcagaacaaggacaatgggaaatatttgaaggccgttactgagcatgaagaaatttatatggaggaagagagcaatgtcaagaaatggaccgctgctttggcaagcttggccaacctttccggctacgttcttcaagatggaatggacgaggcaaatgtcatcaaaagtatcatttctgcaacagcaaaacatgtatcttataaaggttcaaatgttgacaagagcttcctaggcacagatcacctcgttaatggtttgttacacaatcacatcaaatatgagtccttccagccgtcatcttcttcctttcctcacaaatcacacaaatacgatgtctttttgagtttcagaggggaagacacgcgctcgaattttgtgagccacttacatgctgcattagagaggaaaggaattgtcacctttaaggatgacataaggcttgagagaggaagatccatttctccagaacttttgaaagctattgaggattcaaaagttgcgcttgtcattctttcacgaaactatggttcttcaacatggtgcttgaatgagctgcagaagattatgcaatgcagagaagatatggcacaagtggtcatgccagtcttttaccaactgcagccttctgttatacggaagaattttttgaggcagaaaaatgaggggaagttcttgcaggcctccactgaacgtgaattagaagggaaagagagcaaagtacaggaatggagcgatgctttgacaaacctggccaatctttcaggatttgttgttgaagatggcattgatgaggcaaatgccatcagacatattgttcgcgcaacagaaaaacatatgtcatacacatgttcgaatgttgacaagggcttcgtaggaatggatcgccgcgttgaagatcttgtacataatcacatatgtccatggtttaatggcgtgcgaattatagggatccatggaatgcgaggaattggaaagacaacccttgctcgagctgtttatgatcgaatttgtgaagattttgacagtagatgctttctttctaatgttagagaattttctaaaaaagatggcctagtttccttgcaagaaaaacttctttccaggatcctcatggaaaaaactggaactgtagaggatgaatataatggtgctgctatgatacaaaaatgcctgtgtagaataagggtgcttcttgttcttgatgatgtggatcaattgacacaattagaaaaactagctggacatcctaactggttcggtccgggcagtagaattatcataacaagcacagatattcatatgctgaacacgcatggtgttgatgctgtctatcaggctactgtgttagatcctactgaagcacttcaacttatgagtttaaaggcttttaagaaaattcccccgcctgaaaattatttggagctatgccacagcattttaacctatgctcatggacttcccttggctcttgtggttatgggttgctttctgtatggtaaaagcaccaatgaatggagaagtgcaatagatatgctcaagaaaacacctgaaaaaagtatcactgatgtgcttcatataagttttgatggactaagggaaatagaaaaagaagtattcctacatattgcttgtttttataagggcaaggataggaatcgtgtgacagaaatactagactattgtcagcttcaccctgacattaatttaagagttcttgttgataaatctctcattactatttcaaacaacgaaatctgcatgcatgatttgctacaagaaatgggaagggaaatagttcgtcaagagtcccctaaagagccaggcaaacgtagtagactatggtcacatgaagacatacacaacgtgctgaagaaaaatgagggaacagatgcaattcaaggcattgtaatggacttgcctgaattagaaatggctcattggcacccagaagccttctcaaatttgtctaaacttactcttctccatattcataatgtcaatctctcgaagggcctcacttgtctttctagctccttgagacttgtggaatggattgggtaccccttaagatctctcccacaaaatttcaaaccagatgaacttattgaacttaacttgtgccacagcaacattgatcagctttggaagggaaaacagtgtttggacaagttgaagctcctcaaactctgccattcccataacattgtcaaaacgccagacttcacgggcgttcaaaatcttgagacattagatcttgaaggatgcataaatctgacacgagttcatcaatcccttcgatttctaaagtggcttgttgtactgaatcttaaagactgcaaaagtcttgagagcctgccaggtaaaatcgaatcacagtctcttgaaacttttattctttctggctgctcaaaagttaagaagatccctgagtttgttgaaaatatgaaacacttgtcgacgctttgtctcgacaagactgccataaaagaactaccttcctcaattgaacacctcagtggccttgcttctttggatctaagcaactgcaaagaccttgtttcacttccgagtacaattcattcgttgaagtctcttaaaaagctgaatctttctggatgcttgaaacttggccaaagccatgccagtgtaacagaatggaaggatcttttaagacacggttgtgaagtggtttgggaatatttctcatggtccttgtcttctgtatggatgcaaagcgacgatacagaaccaactagtatgccgttgtctgtttctgatctgtgtagctctacagagcaaagaatgaacagatcactgagcatgcggttgcctttatttggtctttgtaatttaacagatctaaacctaagtaactgcaatcttggtgatacagcatttgccaacaacttcggctactttccctctttactggcattaaatctcagtggaaataatttttctaagcttcccccaggcatcagatcatgtttgaaactagagaagattaacttggagaactgcatgagtcttcaagacttgtctggccttccttcaaatagtacattggatataagagcagatggttgttcttcactggaaatgttgcttcatgcattcaattttgatagattgaacatatcatatttgaatttcatcaactgcttcaaaataaaggacaatcaaggctgcaatagcatgtcagttgaaatgctgaagttattccttttccagggcatctctaatgcaaagcaaacttttcaagttgtacttcctggaaggaaaattcccagctggttcagccatggaaggcctggagtttcgttaagtcatagggaaacgccagatttcgatttctgggataccaaatctttgatgggttttgctctctgtgttgtgtttagactccatgagcaccatcacattaatgaactcaacacagatcaattcaagacatttaaagctacacatcatctggtatgttgcctgaaagtcaatggaagagaactagaagtgtgtggcagacaacctgcgtttcgctttagtgaagaattttgccgggttgagtcgcatcacatttggctattctatgttccctgcgattactttggtacagagtggaagactagcaatcggattcagttcttatttgaaaccaaagggcctggcctgaaggtggaagaatgtggtgtccgtccgatatatgaacaagatgtgcaagagatgatcaaaacaatgaatcactccaacagcaggattgaagcactttcaccttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1666

Amino Acids

189.74

Weight (kDa)

6.11

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1428, 2865
Acc36I ACCTGC 1 cut(s) 4762
Acc65I GGTACC 1 cut(s) 3285
AccB1I GGYRCC 2 cut(s) 3168, 3285
AccB7I CCANNNNNTGG 1 cut(s) 2739
AccI GTMKAC 2 cut(s) 3061, 3683
AccII CGCG 4 cut(s) 230, 1591, 2046, 2115
AciI CCGC 6 cut(s) 71, 230, 1317, 2113, 2630, 4051
AcoI YGGCCR 5 cut(s) 352, 732, 1335, 1977, 3856
AcuI CTGAAG 4 cut(s) 2604, 3116, 4460, 4916
AdeI CACNNNGTG 1 cut(s) 3091
AfiI CCNNNNNNNGG 6 cut(s) 350, 2739, 4064, 4456, 4900, 4977
AflII CTTAAG 1 cut(s) 3292
AflIII ACRYGT 3 cut(s) 814, 1417, 2072
AhdI GACNNNNNGTC 1 cut(s) 3690
AhlI ACTAGT 5 cut(s) 514, 637, 1045, 1171, 3980
AjuI GAANNNNNNNTTGG 2 cut(s) 4220, 4252
AleI CACNNNNGTG 3 cut(s) 2792, 3741, 3906
Alw21I GWGCWC 3 cut(s) 147, 240, 4641
Ama87I CYCGRG 3 cut(s) 406, 937, 2214
ApeKI GCWGC 9 cut(s) 431, 962, 1319, 1619, 1780, 1846, 2378, 3630, 4413
AseI ATTAAT 2 cut(s) 2928, 4650
Asp700I GAANNNNTTC 4 cut(s) 2319, 2589, 3021, 4445
Asp718I GGTACC 1 cut(s) 3285
AspA2I CCTAGG 2 cut(s) 853, 1456
AspLEI GCGC 4 cut(s) 224, 1593, 1729, 2048
AspS9I GGNCC 9 cut(s) 13, 82, 262, 711, 1314, 2486, 3238, 3936, 4886
AsuC2I CCSGG 3 cut(s) 665, 2490, 4784
AsuHPI GGTGA 7 cut(s) 179, 857, 1460, 1636, 2909, 4124, 4986
AsuII TTCGAA 1 cut(s) 2078
AvaI CYCGRG 3 cut(s) 406, 937, 2214
AvaII GGWCC 7 cut(s) 13, 82, 262, 711, 1314, 2486, 3936
AvrII CCTAGG 2 cut(s) 853, 1456
BalI TGGCCA 5 cut(s) 354, 734, 1337, 1979, 3858
BamHI GGATCC 2 cut(s) 2175, 2331
BanI GGYRCC 2 cut(s) 3168, 3285
BarI GAAGNNNNNNTAC 4 cut(s) 2112, 2144, 2829, 2861
BauI CACGAG 2 cut(s) 564, 3504
BbsI GAAGAC 5 cut(s) 1590, 3084, 3936, 4247, 4853
Bbv12I GWGCWC 3 cut(s) 147, 240, 4641
BbvI GCAGC 9 cut(s) 443, 974, 1306, 1606, 1767, 1858, 2365, 3617, 4400
BceAI ACGGC 5 cut(s) 594, 1125, 1244, 1504, 3973
BcgI CGANNNNNNTGC 2 cut(s) 3686, 3720
BciVI GTATCC 2 cut(s) 122, 4579
BclI TGATCA 2 cut(s) 3361, 4950
BcnI CCSGG 3 cut(s) 665, 2490, 4784
BcuI ACTAGT 5 cut(s) 514, 637, 1045, 1171, 3980
BfmI CTRYAG 5 cut(s) 1145, 1781, 1844, 2355, 4017
BfrI CTTAAG 1 cut(s) 3292
BfuAI ACCTGC 1 cut(s) 4762
BfuI GTATCC 2 cut(s) 122, 4579
BglII AGATCT 4 cut(s) 2122, 3296, 3478, 4084
BlnI CCTAGG 2 cut(s) 853, 1456
BmcAI AGTACT 2 cut(s) 642, 1176
Bme18I GGWCC 7 cut(s) 13, 82, 262, 711, 1314, 2486, 3936
BmeRI GACNNNNNGTC 1 cut(s) 3690
BmeT110I CYCGRG 3 cut(s) 406, 937, 2214
BmgT120I GGNCC 9 cut(s) 13, 82, 262, 711, 1314, 2486, 3238, 3936, 4886
BmiI GGNNCC 5 cut(s) 263, 2177, 2333, 3170, 3287
BoxI GACNNNNGTC 2 cut(s) 2907, 3067
BpiI GAAGAC 5 cut(s) 1590, 3084, 3936, 4247, 4853
BpmI CTGGAG 2 cut(s) 1674, 4560
Bpu14I TTCGAA 1 cut(s) 2078
BpuEI CTTGAG 5 cut(s) 1688, 2747, 3283, 3488, 3593
BpuMI CCSGG 3 cut(s) 665, 2490, 4784
BsaBI GATNNNNATC 2 cut(s) 2436, 2880
BsaI GGTCTC 1 cut(s) 460
BsaWI WCCGGW 1 cut(s) 207
Bsc4I CCNNNNNNNGG 6 cut(s) 350, 2739, 4064, 4456, 4900, 4977
Bse1I ACTGG 6 cut(s) 1829, 2353, 2483, 3870, 4161, 4326
Bse3DI GCAATG 2 cut(s) 1306, 1802
Bse8I GATNNNNATC 2 cut(s) 2436, 2880
BseGI GGATG 9 cut(s) 238, 601, 1132, 1660, 2368, 2470, 3494, 3848, 3960
BseJI GATNNNNATC 2 cut(s) 2436, 2880
BseLI CCNNNNNNNGG 6 cut(s) 350, 2739, 4064, 4456, 4900, 4977
BseMI GCAATG 2 cut(s) 1306, 1802
BseMII CTCAG 5 cut(s) 1257, 3645, 3754, 4034, 4183
BseNI ACTGG 6 cut(s) 1829, 2353, 2483, 3870, 4161, 4326
BseRI GAGGAG 1 cut(s) 3395
BseXI GCAGC 9 cut(s) 443, 974, 1306, 1606, 1767, 1858, 2365, 3617, 4400
BseYI CCCAGC 1 cut(s) 4514
BsgI GTGCAG 2 cut(s) 640, 1171
Bsh1236I CGCG 4 cut(s) 230, 1591, 2046, 2115
BshNI GGYRCC 2 cut(s) 3168, 3285
BsiHKAI GWGCWC 3 cut(s) 147, 240, 4641
BsiHKCI CYCGRG 3 cut(s) 406, 937, 2214
BsiSI CCGG 6 cut(s) 208, 665, 744, 1347, 2489, 4783
BslFI GGGAC 3 cut(s) 275, 304, 3019
BslI CCNNNNNNNGG 6 cut(s) 350, 2739, 4064, 4456, 4900, 4977
BsmBI CGTCTC 1 cut(s) 409
BsmFI GGGAC 3 cut(s) 275, 304, 3019
BsmI GAATGC 2 cut(s) 2190, 4340
Bso31I GGTCTC 1 cut(s) 460
BsoBI CYCGRG 3 cut(s) 406, 937, 2214
Bsp119I TTCGAA 1 cut(s) 2078
Bsp1286I GDGCHC 3 cut(s) 147, 240, 4641
Bsp1407I TGTACA 1 cut(s) 2128
Bsp19I CCATGG 3 cut(s) 2147, 2179, 4528
BspACI CCGC 6 cut(s) 71, 230, 1317, 2113, 2630, 4051
BspCNI CTCAG 5 cut(s) 1258, 3646, 3753, 4035, 4182
BspFNI CGCG 4 cut(s) 230, 1591, 2046, 2115
BspLI GGNNCC 5 cut(s) 263, 2177, 2333, 3170, 3287
BspMAI CTGCAG 2 cut(s) 1785, 1848
BspMI ACCTGC 1 cut(s) 4762
BspT104I TTCGAA 1 cut(s) 2078
BspT107I GGYRCC 2 cut(s) 3168, 3285
BspTI CTTAAG 1 cut(s) 3292
BspTNI GGTCTC 1 cut(s) 460
BsrDI GCAATG 2 cut(s) 1306, 1802
BsrGI TGTACA 1 cut(s) 2128
BsrI ACTGG 6 cut(s) 1829, 2353, 2483, 3870, 4161, 4326
BssSI CACGAG 2 cut(s) 564, 3504
BssT1I CCWWGG 6 cut(s) 853, 1456, 2147, 2179, 2688, 4528
Bst2BI CACGAG 2 cut(s) 564, 3504
Bst6I CTCTTC 3 cut(s) 1287, 3210, 4720
BstAFI CTTAAG 1 cut(s) 3292
BstAPI GCANNNNNTGC 1 cut(s) 2995
BstAUI TGTACA 1 cut(s) 2128
BstBI TTCGAA 1 cut(s) 2078
BstC8I GCNNGC 7 cut(s) 220, 1330, 1905, 2162, 2987, 3581, 4049
BstDSI CCRYGG 3 cut(s) 2147, 2179, 4528
BstEII GGTNACC 1 cut(s) 167
BstENI CCTNNNNNAGG 1 cut(s) 4454
BstF5I GGATG 9 cut(s) 238, 601, 1132, 1660, 2368, 2470, 3494, 3848, 3960
BstFNI CGCG 4 cut(s) 230, 1591, 2046, 2115
BstHHI GCGC 4 cut(s) 224, 1593, 1729, 2048
BstMWI GCNNNNNNNGC 8 cut(s) 855, 1325, 1334, 1458, 2536, 2995, 4419, 4524
BstNSI RCATGY 7 cut(s) 818, 1421, 1619, 2076, 2989, 4051, 4426
BstPAI GACNNNNGTC 2 cut(s) 2907, 3067
BstPI GGTNACC 1 cut(s) 167
BstSFI CTRYAG 5 cut(s) 1145, 1781, 1844, 2355, 4017
BstUI CGCG 4 cut(s) 230, 1591, 2046, 2115
BstV1I GCAGC 9 cut(s) 443, 974, 1306, 1606, 1767, 1858, 2365, 3617, 4400
BstV2I GAAGAC 5 cut(s) 1590, 3084, 3936, 4247, 4853
BsuI GTATCC 2 cut(s) 122, 4579
BtgI CCRYGG 3 cut(s) 2147, 2179, 4528
BtgZI GCGATG 1 cut(s) 1974
BtsCI GGATG 9 cut(s) 238, 601, 1132, 1660, 2368, 2470, 3494, 3848, 3960
BtsI GCAGTG 1 cut(s) 319
BveI ACCTGC 1 cut(s) 4762
Cac8I GCNNGC 7 cut(s) 220, 1330, 1905, 2162, 2987, 3581, 4049
CfoI GCGC 4 cut(s) 224, 1593, 1729, 2048
Cfr13I GGNCC 9 cut(s) 13, 82, 262, 711, 1314, 2486, 3238, 3936, 4886
CpoI CGGWCCG 1 cut(s) 2486
CseI GACGC 1 cut(s) 3694
CspI CGGWCCG 1 cut(s) 2486
DraI TTTAAA 3 cut(s) 994, 2607, 4684
DraIII CACNNNGTG 1 cut(s) 3091
DriI GACNNNNNGTC 1 cut(s) 3690
EaeI YGGCCR 5 cut(s) 352, 732, 1335, 1977, 3856
Eam1104I CTCTTC 3 cut(s) 1287, 3210, 4720
Eam1105I GACNNNNNGTC 1 cut(s) 3690
EarI CTCTTC 3 cut(s) 1287, 3210, 4720
Eco130I CCWWGG 6 cut(s) 853, 1456, 2147, 2179, 2688, 4528
Eco147I AGGCCT 2 cut(s) 1907, 4537
Eco31I GGTCTC 1 cut(s) 460
Eco32I GATATC 1 cut(s) 881
Eco47I GGWCC 7 cut(s) 13, 82, 262, 711, 1314, 2486, 3936
Eco57I CTGAAG 4 cut(s) 2604, 3116, 4460, 4916
Eco88I CYCGRG 3 cut(s) 406, 937, 2214
Eco91I GGTNACC 1 cut(s) 167
EcoNI CCTNNNNNAGG 1 cut(s) 4454
EcoO109I RGGNCCY 3 cut(s) 262, 3238, 4886
EcoO65I GGTNACC 1 cut(s) 167
EcoRI GAATTC 2 cut(s) 491, 1022
EcoRV GATATC 1 cut(s) 881
EcoT14I CCWWGG 6 cut(s) 853, 1456, 2147, 2179, 2688, 4528
EcoT22I ATGCAT 3 cut(s) 2991, 3494, 4342
ErhI CCWWGG 6 cut(s) 853, 1456, 2147, 2179, 2688, 4528
Esp3I CGTCTC 1 cut(s) 409
FaqI GGGAC 3 cut(s) 275, 304, 3019
FauI CCCGC 1 cut(s) 2637
FauNDI CATATG 3 cut(s) 2061, 2141, 2526
FbaI TGATCA 2 cut(s) 3361, 4950
FblI GTMKAC 2 cut(s) 3061, 3683
FokI GGATG 9 cut(s) 245, 608, 1139, 1667, 2375, 2457, 3501, 3855, 3967
GlaI GCGC 4 cut(s) 223, 1592, 1728, 2047
GsaI CCCAGC 1 cut(s) 4518
GsuI CTGGAG 2 cut(s) 1674, 4560
HapII CCGG 6 cut(s) 208, 665, 744, 1347, 2489, 4783
HgaI GACGC 1 cut(s) 3694
HhaI GCGC 4 cut(s) 224, 1593, 1729, 2048
Hin6I GCGC 4 cut(s) 222, 1591, 1727, 2046
HinP1I GCGC 4 cut(s) 222, 1591, 1727, 2046
HincII GTYRAC 4 cut(s) 841, 1444, 2086, 3684
HindII GTYRAC 4 cut(s) 841, 1444, 2086, 3684
HindIII AAGCTT 2 cut(s) 1330, 4190
HpaII CCGG 6 cut(s) 208, 665, 744, 1347, 2489, 4783
HphI GGTGA 7 cut(s) 179, 857, 1460, 1636, 2909, 4124, 4986
Hpy166II GTNNAC 5 cut(s) 841, 1444, 2086, 3062, 3684
Hpy8I GTNNAC 5 cut(s) 841, 1444, 2086, 3062, 3684
HpyCH4IV ACGT 5 cut(s) 750, 1353, 1918, 3055, 3090
HpyF10VI GCNNNNNNNGC 8 cut(s) 855, 1325, 1334, 1458, 2536, 2995, 4419, 4524
HpySE526I ACGT 5 cut(s) 750, 1353, 1918, 3055, 3090
HspAI GCGC 4 cut(s) 222, 1591, 1727, 2046
KpnI GGTACC 1 cut(s) 3289
Ksp22I TGATCA 2 cut(s) 3361, 4950
LmnI GCTCC 4 cut(s) 1956, 2647, 3263, 3408
Lsp1109I GCAGC 9 cut(s) 443, 974, 1306, 1606, 1767, 1858, 2365, 3617, 4400
MaeII ACGT 5 cut(s) 750, 1353, 1918, 3055, 3090
MfeI CAATTG 2 cut(s) 2441, 3729
MhlI GDGCHC 3 cut(s) 147, 240, 4641
MlsI TGGCCA 5 cut(s) 354, 734, 1337, 1979, 3858
MluNI TGGCCA 5 cut(s) 354, 734, 1337, 1979, 3858
MlyI GAGTC 8 cut(s) 326, 530, 1061, 1517, 3041, 4261, 4623, 4799
MmeI TCCRAC 1 cut(s) 4995
Mox20I TGGCCA 5 cut(s) 354, 734, 1337, 1979, 3858
Mph1103I ATGCAT 3 cut(s) 2991, 3494, 4342
MroXI GAANNNNTTC 4 cut(s) 2319, 2589, 3021, 4445
MscI TGGCCA 5 cut(s) 354, 734, 1337, 1979, 3858
Msp20I TGGCCA 5 cut(s) 354, 734, 1337, 1979, 3858
MspA1I CMGCKG 2 cut(s) 1319, 4518
MspCI CTTAAG 1 cut(s) 3292
MspI CCGG 6 cut(s) 208, 665, 744, 1347, 2489, 4783
MunI CAATTG 2 cut(s) 2441, 3729
Mva1269I GAATGC 2 cut(s) 2190, 4340
MvnI CGCG 4 cut(s) 230, 1591, 2046, 2115
MwoI GCNNNNNNNGC 8 cut(s) 855, 1325, 1334, 1458, 2536, 2995, 4419, 4524
NciI CCSGG 3 cut(s) 665, 2490, 4784
NcoI CCATGG 3 cut(s) 2147, 2179, 4528
NdeI CATATG 3 cut(s) 2061, 2141, 2526
NlaIV GGNNCC 5 cut(s) 263, 2177, 2333, 3170, 3287
NmuCI GTSAC 7 cut(s) 167, 173, 479, 1010, 1642, 2887, 3069
NsiI ATGCAT 3 cut(s) 2991, 3494, 4342
NspI RCATGY 7 cut(s) 818, 1421, 1619, 2076, 2989, 4051, 4426
NspV TTCGAA 1 cut(s) 2078
OliI CACNNNNGTG 3 cut(s) 2792, 3741, 3906
PaeI GCATGC 2 cut(s) 2989, 4051
PaeR7I CTCGAG 3 cut(s) 406, 937, 2214
PasI CCCWGGG 1 cut(s) 323
PceI AGGCCT 2 cut(s) 1907, 4537
PciI ACATGT 3 cut(s) 814, 1417, 2072
PcsI WCGNNNNNNNCGW 9 cut(s) 99, 105, 108, 111, 114, 183, 186, 276, 399
PctI GAATGC 2 cut(s) 2190, 4340
PdmI GAANNNNTTC 4 cut(s) 2319, 2589, 3021, 4445
PfeI GAWTC 9 cut(s) 610, 1141, 1715, 2881, 3550, 3596, 3832, 4861, 4963
PflMI CCANNNNNTGG 1 cut(s) 2739
PfoI TCCNGGA 2 cut(s) 2327, 4498
PleI GAGTC 8 cut(s) 325, 530, 1061, 1516, 3040, 4260, 4623, 4798
PpsI GAGTC 8 cut(s) 325, 530, 1061, 1516, 3040, 4260, 4623, 4798
PpuMI RGGWCCY 1 cut(s) 262
PscI ACATGT 3 cut(s) 814, 1417, 2072
PshAI GACNNNNGTC 2 cut(s) 2907, 3067
PshBI ATTAAT 2 cut(s) 2928, 4650
PsiI TTATAA 2 cut(s) 1428, 2865
Psp5II RGGWCCY 1 cut(s) 262
PspEI GGTNACC 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 4514
PspN4I GGNNCC 5 cut(s) 263, 2177, 2333, 3170, 3287
PspPI GGNCC 9 cut(s) 13, 82, 262, 711, 1314, 2486, 3238, 3936, 4886
PspPPI RGGWCCY 1 cut(s) 262
PspXI VCTCGAGB 1 cut(s) 2214
PstI CTGCAG 2 cut(s) 1785, 1848
PvuII CAGCTG 1 cut(s) 4518
Rsr2I CGGWCCG 1 cut(s) 2486
RsrII CGGWCCG 1 cut(s) 2486
SalI GTCGAC 1 cut(s) 3682
Sau96I GGNCC 9 cut(s) 13, 82, 262, 711, 1314, 2486, 3238, 3936, 4886
ScaI AGTACT 2 cut(s) 642, 1176
SchI GAGTC 8 cut(s) 326, 530, 1061, 1517, 3041, 4261, 4623, 4799
SduI GDGCHC 3 cut(s) 147, 240, 4641
SfcI CTRYAG 5 cut(s) 1145, 1781, 1844, 2355, 4017
Sfr274I CTCGAG 3 cut(s) 406, 937, 2214
SfuI TTCGAA 1 cut(s) 2078
SinI GGWCC 7 cut(s) 13, 82, 262, 711, 1314, 2486, 3936
SlaI CTCGAG 3 cut(s) 406, 937, 2214
SmlI CTYRAG 9 cut(s) 406, 937, 1667, 2214, 2762, 3262, 3292, 3467, 3572
SmoI CTYRAG 9 cut(s) 406, 937, 1667, 2214, 2762, 3262, 3292, 3467, 3572
SpeI ACTAGT 5 cut(s) 514, 637, 1045, 1171, 3980
SphI GCATGC 2 cut(s) 2989, 4051
SseBI AGGCCT 2 cut(s) 1907, 4537
SsiI CCGC 6 cut(s) 71, 230, 1317, 2113, 2630, 4051
SspI AATATT 3 cut(s) 701, 1250, 3926
StuI AGGCCT 2 cut(s) 1907, 4537
StyI CCWWGG 6 cut(s) 853, 1456, 2147, 2179, 2688, 4528
TaiI ACGT 5 cut(s) 753, 1356, 1921, 3058, 3093
TaqII GACCGA 2 cut(s) 728, 2474
TatI WGTACW 8 cut(s) 640, 1174, 1945, 2128, 3544, 3797, 4286, 4492
TauI GCSGC 2 cut(s) 74, 2115
TfiI GAWTC 9 cut(s) 610, 1141, 1715, 2881, 3550, 3596, 3832, 4861, 4963
TseFI GTSAC 7 cut(s) 167, 173, 479, 1010, 1642, 2887, 3069
TseI GCWGC 9 cut(s) 431, 962, 1319, 1619, 1780, 1846, 2378, 3630, 4413
Tsp45I GTSAC 7 cut(s) 167, 173, 479, 1010, 1642, 2887, 3069
TspGWI ACGGA 3 cut(s) 239, 1876, 4907
Van91I CCANNNNNTGG 1 cut(s) 2739
Vha464I CTTAAG 1 cut(s) 3292
VpaK11BI GGWCC 7 cut(s) 13, 82, 262, 711, 1314, 2486, 3936
VspI ATTAAT 2 cut(s) 2928, 4650
XagI CCTNNNNNAGG 1 cut(s) 4454
XceI RCATGY 7 cut(s) 818, 1421, 1619, 2076, 2989, 4051, 4426
XcmI CCANNNNNNNNNTGG 3 cut(s) 2335, 4535, 4597
XhoI CTCGAG 3 cut(s) 406, 937, 2214
XmaJI CCTAGG 2 cut(s) 853, 1456
XmiI GTMKAC 2 cut(s) 3061, 3683
XmnI GAANNNNTTC 4 cut(s) 2319, 2589, 3021, 4445
ZrmI AGTACT 2 cut(s) 642, 1176
Zsp2I ATGCAT 3 cut(s) 2991, 3494, 4342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.