Rroxscaffold_4G00326940

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Forward (+)
59214779 .. 59217346
2568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326940.1

Sequence Viewer

Length: 975 bp
ATGGAGGTTAGATCTGAGGGTGTACAGCTCCGATGCGACAAGCTTCCTGGCAGTTGGGGAGCTATGGAATCCGCATTTGCTCTCCAACTTAACCAATTGGATATTGCAAATTACACAGGTTCTAGGTCAAGGGGTTCTATCTTCATCGGCGGCGAGAATATACCCTTGGAATGCATCATTCACTTCGACAAGCACAGCTCCTCTGCTCGAATTAGAAAATGGGGGTATCAATGTTTAAGAGGAAGTCGGAGGTTGCTTTCTAAGCTTGAGAACTTTAACTTAGAGAATTGCAAGAGACTGCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTTGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTAAACAGACTAGACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAACCAAGGATGCGATAACATAGTATTTGAAATGCTGAAGACATTCATGTATCAGCTAATGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATAAGCACCATCTGGTGGATAAGCTTCCTAGAGATGAATTCAAGATTTTTAATGCAACACATCATCTTGTATGTTGCTTGAAGCTTGATGGAAGAAAATTGGAAGTATATGGCAAACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTAAGTCAGATCACCTGTGGATGTCCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGTTGGCATAACAGTTGCAGTCAGCTTGAGTTCTTATTTAAAACCAGAGGGCCGGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTCATATATAAGCAAGATGTGCAAGAGTTGAACCAAACAACCACTCAATCAAGCAGTAGGGTGTCTCCTGATGACGATATATTGATTGGTTTTGACATTCCGGTTGCAGGGGAAACCAGTGGCACTGGTAGCAGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

324

Amino Acids

36.97

Weight (kDa)

8.15

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 161 - 286 5.3e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 553
AccI GTMKAC 1 cut(s) 331
AciI CCGC 2 cut(s) 72, 150
AcsI RAATTY 3 cut(s) 388, 575, 680
AcuI CTGAAG 2 cut(s) 503, 830
AfaI GTAC 4 cut(s) 24, 358, 534, 745
AfiI CCNNNNNNNGG 2 cut(s) 553, 941
AgsI TTSAA 6 cut(s) 418, 436, 476, 580, 619, 865
AjnI CCWGG 2 cut(s) 46, 687
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
Alw26I GTCTC 3 cut(s) 289, 839, 903
AoxI GGCC 1 cut(s) 800
ApoI RAATTY 3 cut(s) 388, 575, 680
Asp700I GAANNNNTTC 1 cut(s) 488
AspS9I GGNCC 1 cut(s) 800
AsuC2I CCSGG 1 cut(s) 804
AsuHPI GGTGA 2 cut(s) 695, 826
BauI CACGAG 1 cut(s) 726
BbsI GAAGAC 1 cut(s) 491
BccI CCATC 4 cut(s) 328, 344, 555, 620
BciT130I CCWGG 2 cut(s) 48, 689
BcnI CCSGG 1 cut(s) 804
BcoDI GTCTC 3 cut(s) 289, 839, 903
BfaI CTAG 3 cut(s) 123, 401, 567
BglII AGATCT 1 cut(s) 11
BisI GCNGC 1 cut(s) 151
BlsI GCNGC 1 cut(s) 152
Bme1390I CCNGG 3 cut(s) 48, 689, 804
BmgT120I GGNCC 1 cut(s) 800
BmrFI CCNGG 3 cut(s) 48, 689, 804
BmsI GCATC 5 cut(s) 23, 183, 370, 392, 446
BpiI GAAGAC 1 cut(s) 491
BplI GAGNNNNNCTC 2 cut(s) 819, 851
BpuEI CTTGAG 2 cut(s) 287, 797
BpuMI CCSGG 1 cut(s) 804
BsaJI CCNNGG 2 cut(s) 165, 451
BsaWI WCCGGW 1 cut(s) 934
Bsc4I CCNNNNNNNGG 2 cut(s) 553, 941
Bse1I ACTGG 2 cut(s) 951, 964
BseBI CCWGG 2 cut(s) 48, 689
BseDI CCNNGG 2 cut(s) 165, 451
BseGI GGATG 2 cut(s) 461, 717
BseLI CCNNNNNNNGG 2 cut(s) 553, 941
BseMII CTCAG 1 cut(s) 6
BseNI ACTGG 2 cut(s) 951, 964
BseRI GAGGAG 1 cut(s) 190
BshFI GGCC 1 cut(s) 802
BsiSI CCGG 2 cut(s) 803, 935
BslI CCNNNNNNNGG 2 cut(s) 553, 941
BsmAI GTCTC 3 cut(s) 289, 839, 903
BsmBI CGTCTC 1 cut(s) 839
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 1 cut(s) 802
Bsp1407I TGTACA 1 cut(s) 22
Bsp143I GATC 2 cut(s) 11, 700
BspACI CCGC 2 cut(s) 72, 150
BspANI GGCC 1 cut(s) 802
BspCNI CTCAG 1 cut(s) 7
BsrGI TGTACA 1 cut(s) 22
BsrI ACTGG 2 cut(s) 951, 964
BssECI CCNNGG 2 cut(s) 165, 451
BssMI GATC 2 cut(s) 11, 700
BssNAI GTATAC 1 cut(s) 332
BssSI CACGAG 1 cut(s) 726
BssT1I CCWWGG 2 cut(s) 165, 451
Bst1107I GTATAC 1 cut(s) 332
Bst2BI CACGAG 1 cut(s) 726
Bst2UI CCWGG 2 cut(s) 48, 689
Bst4CI ACNGT 1 cut(s) 764
BstAPI GCANNNNNTGC 1 cut(s) 853
BstAUI TGTACA 1 cut(s) 22
BstC8I GCNNGC 1 cut(s) 660
BstDEI CTNAG 3 cut(s) 15, 261, 280
BstF5I GGATG 2 cut(s) 461, 717
BstKTI GATC 2 cut(s) 14, 703
BstMAI GTCTC 3 cut(s) 289, 839, 903
BstMBI GATC 2 cut(s) 11, 700
BstMWI GCNNNNNNNGC 4 cut(s) 262, 521, 853, 963
BstNI CCWGG 2 cut(s) 48, 689
BstSCI CCNGG 3 cut(s) 46, 687, 802
BstV2I GAAGAC 1 cut(s) 491
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
BstZ17I GTATAC 1 cut(s) 332
BsuRI GGCC 1 cut(s) 802
BtsCI GGATG 2 cut(s) 461, 717
BtsIMutI CAGTG 3 cut(s) 362, 957, 958
Cac8I GCNNGC 1 cut(s) 660
Cfr13I GGNCC 1 cut(s) 800
Csp6I GTAC 4 cut(s) 23, 357, 533, 744
CviAII CATG 1 cut(s) 493
CviQI GTAC 4 cut(s) 23, 357, 533, 744
DdeI CTNAG 3 cut(s) 15, 261, 280
DpnI GATC 2 cut(s) 13, 702
DpnII GATC 2 cut(s) 11, 700
DraI TTTAAA 2 cut(s) 393, 790
Eco130I CCWWGG 2 cut(s) 165, 451
Eco57I CTGAAG 2 cut(s) 503, 830
EcoRI GAATTC 1 cut(s) 575
EcoRII CCWGG 2 cut(s) 46, 687
EcoT14I CCWWGG 2 cut(s) 165, 451
EcoT22I ATGCAT 2 cut(s) 176, 385
ErhI CCWWGG 2 cut(s) 165, 451
Esp3I CGTCTC 1 cut(s) 839
FaeI CATG 1 cut(s) 496
FatI CATG 1 cut(s) 492
FblI GTMKAC 1 cut(s) 331
Fnu4HI GCNGC 1 cut(s) 151
FokI GGATG 2 cut(s) 468, 724
Fsp4HI GCNGC 1 cut(s) 151
FspBI CTAG 3 cut(s) 123, 401, 567
GluI GCNGC 1 cut(s) 151
HaeIII GGCC 1 cut(s) 802
HapII CCGG 2 cut(s) 803, 935
Hin1II CATG 1 cut(s) 496
HindIII AAGCTT 4 cut(s) 41, 263, 560, 620
HinfI GANTC 2 cut(s) 68, 828
HpaII CCGG 2 cut(s) 803, 935
HphI GGTGA 2 cut(s) 695, 826
Hpy166II GTNNAC 2 cut(s) 23, 332
Hpy188I TCNGA 6 cut(s) 16, 32, 249, 313, 700, 810
Hpy188III TCNNGA 3 cut(s) 580, 728, 902
Hpy8I GTNNAC 2 cut(s) 23, 332
HpyAV CCTTC 3 cut(s) 326, 805, 811
HpyCH4III ACNGT 1 cut(s) 764
HpyF10VI GCNNNNNNNGC 4 cut(s) 262, 521, 853, 963
HpyF3I CTNAG 3 cut(s) 15, 261, 280
Hsp92II CATG 1 cut(s) 496
Kzo9I GATC 2 cut(s) 11, 700
LmnI GCTCC 3 cut(s) 33, 59, 203
LweI GCATC 5 cut(s) 23, 183, 370, 392, 446
MaeI CTAG 3 cut(s) 123, 401, 567
MalI GATC 2 cut(s) 13, 702
MboI GATC 2 cut(s) 11, 700
MboII GAAGA 4 cut(s) 133, 496, 642, 689
MfeI CAATTG 2 cut(s) 95, 427
MflI RGATCY 1 cut(s) 11
MlyI GAGTC 1 cut(s) 837
MmeI TCCRAC 2 cut(s) 109, 227
MnlI CCTC 5 cut(s) 10, 211, 233, 243, 791
Mph1103I ATGCAT 2 cut(s) 176, 385
MroXI GAANNNNTTC 1 cut(s) 488
MseI TTAA 8 cut(s) 90, 236, 276, 341, 392, 588, 693, 789
MspI CCGG 2 cut(s) 803, 935
MspR9I CCNGG 3 cut(s) 48, 689, 804
MunI CAATTG 2 cut(s) 95, 427
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 2 cut(s) 48, 689
MwoI GCNNNNNNNGC 4 cut(s) 262, 521, 853, 963
NciI CCSGG 1 cut(s) 804
NdeII GATC 2 cut(s) 11, 700
NlaIII CATG 1 cut(s) 496
NsiI ATGCAT 2 cut(s) 176, 385
PctI GAATGC 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 553
PkrI GCNGC 1 cut(s) 152
PleI GAGTC 1 cut(s) 836
PpsI GAGTC 1 cut(s) 836
Psp6I CCWGG 2 cut(s) 46, 687
PspGI CCWGG 2 cut(s) 46, 687
PspPI GGNCC 1 cut(s) 800
PsuI RGATCY 1 cut(s) 11
RsaI GTAC 4 cut(s) 24, 358, 534, 745
RsaNI GTAC 4 cut(s) 23, 357, 533, 744
SaqAI TTAA 8 cut(s) 90, 236, 276, 341, 392, 588, 693, 789
SatI GCNGC 1 cut(s) 151
Sau3AI GATC 2 cut(s) 11, 700
Sau96I GGNCC 1 cut(s) 800
SchI GAGTC 1 cut(s) 837
ScrFI CCNGG 3 cut(s) 48, 689, 804
SfaNI GCATC 5 cut(s) 23, 183, 370, 392, 446
SmlI CTYRAG 2 cut(s) 266, 776
SmoI CTYRAG 2 cut(s) 266, 776
SsiI CCGC 2 cut(s) 72, 150
SspMI CTAG 3 cut(s) 123, 401, 567
StyD4I CCNGG 3 cut(s) 46, 687, 802
StyI CCWWGG 2 cut(s) 165, 451
TaaI ACNGT 1 cut(s) 764
TaqI TCGA 2 cut(s) 186, 208
TatI WGTACW 3 cut(s) 22, 356, 532
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 8 cut(s) 90, 236, 276, 341, 392, 588, 693, 789
Tru9I TTAA 8 cut(s) 90, 236, 276, 341, 392, 588, 693, 789
TscAI CASTG 3 cut(s) 369, 958, 964
TspDTI ATGAA 4 cut(s) 133, 412, 481, 588
TspGWI ACGGA 1 cut(s) 821
TspRI CASTG 3 cut(s) 369, 958, 964
Van91I CCANNNNNTGG 1 cut(s) 553
XapI RAATTY 3 cut(s) 388, 575, 680
XmiI GTMKAC 1 cut(s) 331
XmnI GAANNNNTTC 1 cut(s) 488
XspI CTAG 3 cut(s) 123, 401, 567
Zsp2I ATGCAT 2 cut(s) 176, 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.