Rroxscaffold_4G00327320

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Forward (+)
59931331 .. 59941261
9931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327320.1

Sequence Viewer

Length: 3351 bp
ATGAAAGAGCTGGGCCAACAAGTCCTCCCCGTCTTCGTAGGCGTGGATCCTTCTGATGTGCGGCACCAAAGGGGACATTTTGAGCTCAAAAGGAAAACCCAAGTAGATGTGGAAGTAAGGGAACATGAAGAAGTTTATGGCAAGACTGAGGACAGACTAAATGCGTGGAGAGCTGCTTTGACTCAGGTCGCCAATCTTTCTGGCTGGGATGCCAGGAATTATCGATACGAAGCAAAACTAATTGATGAAATTGTTAATCATATACTAAAGAAGTCCGCGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGGTTCCCGCGTTGATGATTTATTAAGCAATTACATATGTCCCCAGCTTGTTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACGCTTGCTCGAGCTATCCATGATCAAATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGACAAAAATTGAAAATATAGGGGATGAATACACAGGAGCTGCTATGATACAAAGGCGGTTGTGTAGAAAAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAGCTGGTTTGGTCCAGGGACTAGAATTATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAAGTTAACGAGTTAAACTGTGGTGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATGTCCCCCACCAGAAGATTATTTGGATCTGTGCTATGATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTGTCTGGTAGAGCGGCTGATAAATGGGCAAGTGCAATAGATAGGCTGAGAAGCACACCAGATAAACGCATTATTGACGTGCTTCGGATTAGTTTTGATGACCTGCATAAAAAAGAGAAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTACAACCTGTCATTGGTTTAGATGTTCTCGCTGATAGATCTCTCATAACTATCTCCAACAATGATTTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAGCAGTCTCCTGATGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGGCGACATCAACAATGTGCTGAAGAAAAATAACGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACGAGTTGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTGGTCTTCTCCATATTCGTAATGTGGACCTTCCCAAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTAAGATCTCTCCCAAAAGTTTTTGAAGCAGACGAACTTATTGAACTTAACTTGTGCCACAGCAACATTAAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAAAATTGTGGAGACCCCCGACCTCACAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAATCCGTTGGATTTCTAAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAGAGTCTCAAGAGTCTGCCAAGTAGAATTGAACTGGAATCTCTTGAAACATTTATTCTTTCTAACTGCTCAAAAGTTGAGAAGATTCCCGAGTTTGTTGGAAATATGGAACATTTGTTGGTGCTTTGTCTTGATGAGACTGCCATTGAGGAACTGCCTGTATCAATTGAACGGCTGAGTGGCCTTGTGTCATTGAATCTGAGCAACTGCAGAAATCTTGTCCGTCTTCCGAGTACCATCAATAAATTGAAGTCTATTAAAGATCTTAATCTTTCCGGATGCTTGAAACTCGGCAAACATCAGGTAAATGTGGGGGAGATGGACTGTTTTGAGGAAACTGACGTGAATAGTGGGTCTGCAATAGAAATGTCATCTACCCATGATCGCAGAAAATATGTGAGAGGTTCAATCTTTCATGGATGCAAAGTGGTTTGGGGATCTTTAAATAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAATGAGTTTTGGCTTGCCTATATCTCAAAAAGTGAATACAGAGCCAACGAGTTTCCGCTTGCCTATTTCTGGTCTGTGTAATTTAACGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTCGGTTACTTTCCCTCGTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGCGTTCCTTCAGGCATTCGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGGAATGAAGGATGCAATAACATAGCATTTGAAATGCTGATGGCATTCATGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTTAGCCATCAGAGTGTTGGGTGTTCATTAAGTGTATCTCTACCTGTACATTGGAATAACAGTCGGTTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCCGGTGGATAAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTGTGTTGCCTGAAGCTCGATGGAAGAGAATTGGAAGTATATGGCAGACAGCCGGCATTTCGCTTCAGTGAAGAATTTTGCCAGGTTGAATCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCAACTTGAGTTCTCATTTGAAACCAGAGGGCCGGGTCTGAGGGTGAAGGAGTGCGGAGTCCGTCTGATATATGAGCAAGAAGTGCAAGAGTTGAACCAAACAACCACTAAATCAAGCAGTAGGATGTCTCCTTATGCGGATATATTGATTGGTTTTGACATTCCAGTTGCAGGGGAAACCAGTGGCACTGGCAGCACAACTTGCATGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1116

Amino Acids

126.24

Weight (kDa)

6.68

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 98 1.3e-13 TIR domain
NB-ARC PF00931 124 - 278 1.6e-19 NB-ARC domain
AAA_22 PF13401 126 - 225 8.4e-06 AAA domain
NACHT PF05729 128 - 225 9.2e-07 NACHT domain
WHD_ROQ1 PF23282 346 - 416 5.2e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 604 - 690 4.1e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 616 - 685 5.3e-09 Leucine-rich repeat region
C-JID PF20160 923 - 1072 4.7e-21 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 476, 1038
AccB1I GGYRCC 1 cut(s) 63
AccB7I CCANNNNNTGG 2 cut(s) 1342, 2911
AccBSI CCGCTC 1 cut(s) 941
AccI GTMKAC 2 cut(s) 281, 2560
AccII CGCG 3 cut(s) 278, 327, 2463
AccIII TCCGGA 1 cut(s) 2051
AciI CCGC 9 cut(s) 61, 276, 325, 400, 607, 941, 2323, 3183, 3266
AclWI GGATC 8 cut(s) 41, 54, 382, 395, 657, 867, 1101, 2221
AcsI RAATTY 7 cut(s) 444, 1399, 1597, 2405, 2617, 2933, 3038
AcuI CTGAAG 4 cut(s) 1328, 2454, 2996, 3013
AfaI GTAC 5 cut(s) 2011, 2587, 2841, 2892, 3103
AfiI CCNNNNNNNGG 5 cut(s) 1139, 1342, 2336, 2911, 3299
AflII CTTAAG 1 cut(s) 1504
AjiI CACGTC 2 cut(s) 1006, 2119
AjnI CCWGG 5 cut(s) 212, 700, 1258, 2770, 3045
AjuI GAANNNNNNNTTGG 2 cut(s) 2492, 2524
Alw21I GWGCWC 2 cut(s) 87, 2904
AlwI GGATC 8 cut(s) 41, 54, 382, 395, 657, 867, 1101, 2221
AlwNI CAGNNNCTG 1 cut(s) 590
Ama87I CYCGRG 2 cut(s) 426, 1865
Aor13HI TCCGGA 1 cut(s) 2051
AoxI GGCC 5 cut(s) 13, 508, 1450, 1957, 3158
ApeKI GCWGC 4 cut(s) 173, 590, 687, 3321
ApoI RAATTY 7 cut(s) 444, 1399, 1597, 2405, 2617, 2933, 3038
Asp700I GAANNNNTTC 6 cut(s) 319, 531, 801, 1233, 1327, 2754
AspS9I GGNCC 4 cut(s) 13, 698, 1438, 3158
AsuC2I CCSGG 1 cut(s) 3162
AsuHPI GGTGA 5 cut(s) 806, 2396, 2440, 3053, 3184
AvaI CYCGRG 2 cut(s) 426, 1865
AvaII GGWCC 2 cut(s) 698, 1438
BamHI GGATCC 2 cut(s) 46, 387
BanI GGYRCC 1 cut(s) 63
BanII GRGCYC 1 cut(s) 87
BauI CACGAG 2 cut(s) 908, 3084
BbsI GAAGAC 3 cut(s) 25, 1409, 1994
Bbv12I GWGCWC 2 cut(s) 87, 2904
BbvI GCAGC 4 cut(s) 160, 577, 699, 3333
BccI CCATC 9 cut(s) 1731, 2021, 2089, 2557, 2573, 2707, 2808, 2913, 2978
BceAI ACGGC 1 cut(s) 1964
BciT130I CCWGG 5 cut(s) 214, 702, 1260, 2772, 3047
BciVI GTATCC 1 cut(s) 1509
BclI TGATCA 1 cut(s) 439
BcnI CCSGG 1 cut(s) 3162
BfaI CTAG 4 cut(s) 512, 708, 1112, 2564
BfmI CTRYAG 1 cut(s) 1984
BfrI CTTAAG 1 cut(s) 1504
BfuAI ACCTGC 2 cut(s) 476, 1038
BfuI GTATCC 1 cut(s) 1509
BglII AGATCT 4 cut(s) 1163, 1508, 1690, 2038
BisI GCNGC 6 cut(s) 62, 174, 591, 688, 942, 3322
BlsI GCNGC 6 cut(s) 63, 175, 592, 689, 943, 3323
Bme1390I CCNGG 6 cut(s) 214, 702, 1260, 2772, 3047, 3162
Bme18I GGWCC 2 cut(s) 698, 1438
BmeT110I CYCGRG 2 cut(s) 426, 1865
BmgBI CACGTC 2 cut(s) 1006, 2119
BmgT120I GGNCC 4 cut(s) 13, 698, 1438, 3158
BmiI GGNNCC 4 cut(s) 48, 65, 322, 389
BmrFI CCNGG 6 cut(s) 214, 702, 1260, 2772, 3047, 3162
BmrI ACTGGG 1 cut(s) 1503
BmsI GCATC 8 cut(s) 199, 751, 1188, 2045, 2186, 2599, 2621, 2675
BmuI ACTGGG 1 cut(s) 1503
BoxI GACNNNNGTC 1 cut(s) 185
BpiI GAAGAC 3 cut(s) 25, 1409, 1994
Bpu10I CCTNAGC 1 cut(s) 888
BpuEI CTTGAG 5 cut(s) 1495, 1700, 1769, 2516, 3155
BpuMI CCSGG 1 cut(s) 3162
Bsa29I ATCGAT 1 cut(s) 223
BsaBI GATNNNNATC 3 cut(s) 648, 1092, 2043
BsaI GGTCTC 2 cut(s) 1287, 1643
BsaJI CCNNGG 3 cut(s) 391, 701, 1336
BsaWI WCCGGW 2 cut(s) 2051, 2908
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bsc4I CCNNNNNNNGG 5 cut(s) 1139, 1342, 2336, 2911, 3299
Bse118I RCCGGY 1 cut(s) 3016
Bse1I ACTGG 5 cut(s) 1498, 1815, 3293, 3309, 3322
Bse8I GATNNNNATC 3 cut(s) 648, 1092, 2043
BseAI TCCGGA 1 cut(s) 2051
BseBI CCWGG 5 cut(s) 214, 702, 1260, 2772, 3047
BseCI ATCGAT 1 cut(s) 223
BseDI CCNNGG 3 cut(s) 391, 701, 1336
BseGI GGATG 8 cut(s) 214, 580, 1706, 2060, 2201, 2690, 2905, 3258
BseJI GATNNNNATC 3 cut(s) 648, 1092, 2043
BseLI CCNNNNNNNGG 5 cut(s) 1139, 1342, 2336, 2911, 3299
BseMII CTCAG 9 cut(s) 138, 197, 902, 965, 1943, 1967, 2357, 2778, 3158
BseNI ACTGG 5 cut(s) 1498, 1815, 3293, 3309, 3322
BseRI GAGGAG 1 cut(s) 1472
BseXI GCAGC 4 cut(s) 160, 577, 699, 3333
BseYI CCCAGC 4 cut(s) 10, 204, 360, 1224
Bsh1236I CGCG 3 cut(s) 278, 327, 2463
BshFI GGCC 5 cut(s) 15, 510, 1452, 1959, 3160
BshNI GGYRCC 1 cut(s) 63
BshVI ATCGAT 1 cut(s) 223
BsiHKAI GWGCWC 2 cut(s) 87, 2904
BsiHKCI CYCGRG 2 cut(s) 426, 1865
BsiSI CCGG 4 cut(s) 2052, 2909, 3017, 3161
BslFI GGGAC 4 cut(s) 87, 342, 718, 822
BslI CCNNNNNNNGG 5 cut(s) 1139, 1342, 2336, 2911, 3299
BsmFI GGGAC 4 cut(s) 87, 342, 718, 822
BsmI GAATGC 2 cut(s) 2475, 2717
BsnI GGCC 5 cut(s) 15, 510, 1452, 1959, 3160
Bso31I GGTCTC 2 cut(s) 1287, 1643
BsoBI CYCGRG 2 cut(s) 426, 1865
Bsp1286I GDGCHC 2 cut(s) 87, 2904
Bsp13I TCCGGA 1 cut(s) 2051
Bsp1407I TGTACA 1 cut(s) 2839
Bsp19I CCATGG 1 cut(s) 391
BspACI CCGC 9 cut(s) 61, 276, 325, 400, 607, 941, 2323, 3183, 3266
BspANI GGCC 5 cut(s) 15, 510, 1452, 1959, 3160
BspCNI CTCAG 9 cut(s) 139, 196, 901, 966, 1944, 1968, 2358, 2779, 3159
BspDI ATCGAT 1 cut(s) 223
BspEI TCCGGA 1 cut(s) 2051
BspFNI CGCG 3 cut(s) 278, 327, 2463
BspLI GGNNCC 4 cut(s) 48, 65, 322, 389
BspMAI CTGCAG 1 cut(s) 1988
BspMI ACCTGC 2 cut(s) 476, 1038
BspPI GGATC 8 cut(s) 41, 54, 382, 395, 657, 867, 1101, 2221
BspT107I GGYRCC 1 cut(s) 63
BspTI CTTAAG 1 cut(s) 1504
BspTNI GGTCTC 2 cut(s) 1287, 1643
BsrBI CCGCTC 1 cut(s) 941
BsrFI RCCGGY 1 cut(s) 3016
BsrGI TGTACA 1 cut(s) 2839
BsrI ACTGG 5 cut(s) 1498, 1815, 3293, 3309, 3322
BssAI RCCGGY 1 cut(s) 3016
BssECI CCNNGG 3 cut(s) 391, 701, 1336
BssNAI GTATAC 1 cut(s) 282
BssSI CACGAG 2 cut(s) 908, 3084
BssT1I CCWWGG 2 cut(s) 391, 1336
Bst1107I GTATAC 1 cut(s) 282
Bst2BI CACGAG 2 cut(s) 908, 3084
Bst2UI CCWGG 5 cut(s) 214, 702, 1260, 2772, 3047
Bst4CI ACNGT 4 cut(s) 791, 2102, 2855, 3122
Bst6I CTCTTC 1 cut(s) 2983
BstAFI CTTAAG 1 cut(s) 1504
BstAPI GCANNNNNTGC 3 cut(s) 1207, 3211, 3330
BstAUI TGTACA 1 cut(s) 2839
BstC8I GCNNGC 8 cut(s) 398, 423, 1069, 1124, 2282, 2327, 3018, 3335
BstDSI CCRYGG 1 cut(s) 391
BstEII GGTNACC 1 cut(s) 2428
BstF5I GGATG 8 cut(s) 214, 580, 1706, 2060, 2201, 2690, 2905, 3258
BstFNI CGCG 3 cut(s) 278, 327, 2463
BstMWI GCNNNNNNNGC 8 cut(s) 170, 402, 1207, 2491, 2879, 3211, 3321, 3330
BstNI CCWGG 5 cut(s) 214, 702, 1260, 2772, 3047
BstNSI RCATGY 2 cut(s) 400, 3337
BstPAI GACNNNNGTC 1 cut(s) 185
BstPI GGTNACC 1 cut(s) 2428
BstSCI CCNGG 6 cut(s) 212, 700, 1258, 2770, 3045, 3160
BstSFI CTRYAG 1 cut(s) 1984
BstUI CGCG 3 cut(s) 278, 327, 2463
BstV1I GCAGC 4 cut(s) 160, 577, 699, 3333
BstV2I GAAGAC 3 cut(s) 25, 1409, 1994
BstX2I RGATCY 8 cut(s) 46, 387, 859, 1163, 1508, 1690, 2038, 2213
BstXI CCANNNNNNTGG 1 cut(s) 368
BstYI RGATCY 8 cut(s) 46, 387, 859, 1163, 1508, 1690, 2038, 2213
BstZ17I GTATAC 1 cut(s) 282
Bsu15I ATCGAT 1 cut(s) 223
BsuI GTATCC 1 cut(s) 1509
BsuRI GGCC 5 cut(s) 15, 510, 1452, 1959, 3160
BsuTUI ATCGAT 1 cut(s) 223
BtgI CCRYGG 1 cut(s) 391
BtrI CACGTC 2 cut(s) 1006, 2119
BtsCI GGATG 8 cut(s) 214, 580, 1706, 2060, 2201, 2690, 2905, 3258
BtsIMutI CAGTG 4 cut(s) 2591, 3037, 3315, 3316
BveI ACCTGC 2 cut(s) 476, 1038
Cac8I GCNNGC 8 cut(s) 398, 423, 1069, 1124, 2282, 2327, 3018, 3335
CaiI CAGNNNCTG 1 cut(s) 590
Cfr10I RCCGGY 1 cut(s) 3016
Cfr13I GGNCC 4 cut(s) 13, 698, 1438, 3158
ClaI ATCGAT 1 cut(s) 223
Csp6I GTAC 5 cut(s) 2010, 2586, 2840, 2891, 3102
CviQI GTAC 5 cut(s) 2010, 2586, 2840, 2891, 3102
DraI TTTAAA 1 cut(s) 2220
Eam1104I CTCTTC 1 cut(s) 2983
EarI CTCTTC 1 cut(s) 2983
Ecl136II GAGCTC 1 cut(s) 85
Eco130I CCWWGG 2 cut(s) 391, 1336
Eco147I AGGCCT 1 cut(s) 1452
Eco24I GRGCYC 1 cut(s) 87
Eco31I GGTCTC 2 cut(s) 1287, 1643
Eco47I GGWCC 2 cut(s) 698, 1438
Eco53kI GAGCTC 1 cut(s) 85
Eco57I CTGAAG 4 cut(s) 1328, 2454, 2996, 3013
Eco88I CYCGRG 2 cut(s) 426, 1865
Eco91I GGTNACC 1 cut(s) 2428
EcoICRI GAGCTC 1 cut(s) 85
EcoO65I GGTNACC 1 cut(s) 2428
EcoRI GAATTC 2 cut(s) 2405, 2933
EcoRII CCWGG 5 cut(s) 212, 700, 1258, 2770, 3045
EcoT14I CCWWGG 2 cut(s) 391, 1336
EcoT22I ATGCAT 2 cut(s) 1203, 2614
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 2 cut(s) 391, 1336
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FaqI GGGAC 4 cut(s) 87, 342, 718, 822
FauI CCCGC 2 cut(s) 332, 393
FauNDI CATATG 1 cut(s) 353
FbaI TGATCA 1 cut(s) 439
FblI GTMKAC 2 cut(s) 281, 2560
Fnu4HI GCNGC 6 cut(s) 62, 174, 591, 688, 942, 3322
FokI GGATG 8 cut(s) 221, 587, 1713, 2067, 2208, 2697, 2892, 3265
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 6 cut(s) 62, 174, 591, 688, 942, 3322
FspBI CTAG 4 cut(s) 512, 708, 1112, 2564
GluI GCNGC 6 cut(s) 62, 174, 591, 688, 942, 3322
GsaI CCCAGC 4 cut(s) 14, 208, 364, 1228
HaeIII GGCC 5 cut(s) 15, 510, 1452, 1959, 3160
HapII CCGG 4 cut(s) 2052, 2909, 3017, 3161
HincII GTYRAC 5 cut(s) 298, 657, 778, 1356, 3131
HindII GTYRAC 5 cut(s) 298, 657, 778, 1356, 3131
HindIII AAGCTT 3 cut(s) 1748, 2492, 2918
HpaI GTTAAC 1 cut(s) 778
HpaII CCGG 4 cut(s) 2052, 2909, 3017, 3161
HphI GGTGA 5 cut(s) 806, 2396, 2440, 3053, 3184
Hpy166II GTNNAC 8 cut(s) 282, 298, 657, 778, 1356, 1438, 2561, 3131
Hpy8I GTNNAC 8 cut(s) 282, 298, 657, 778, 1356, 1438, 2561, 3131
HpyCH4III ACNGT 4 cut(s) 791, 2102, 2855, 3122
HpyCH4IV ACGT 4 cut(s) 1005, 1267, 2118, 2354
HpyF10VI GCNNNNNNNGC 8 cut(s) 170, 402, 1207, 2491, 2879, 3211, 3321, 3330
HpySE526I ACGT 4 cut(s) 1005, 1267, 2118, 2354
Kpn2I TCCGGA 1 cut(s) 2051
KroI GCCGGC 1 cut(s) 3016
KroNI GCCGGC 1 cut(s) 3018
Ksp22I TGATCA 1 cut(s) 439
KspAI GTTAAC 1 cut(s) 778
LmnI GCTCC 2 cut(s) 587, 2390
Lsp1109I GCAGC 4 cut(s) 160, 577, 699, 3333
LweI GCATC 8 cut(s) 199, 751, 1188, 2045, 2186, 2599, 2621, 2675
MaeI CTAG 4 cut(s) 512, 708, 1112, 2564
MaeII ACGT 4 cut(s) 1005, 1267, 2118, 2354
MaeIII GTNAC 3 cut(s) 1099, 2411, 2428
MbiI CCGCTC 1 cut(s) 941
MfeI CAATTG 2 cut(s) 1941, 2656
MflI RGATCY 8 cut(s) 46, 387, 859, 1163, 1508, 1690, 2038, 2213
MhlI GDGCHC 2 cut(s) 87, 2904
MlyI GAGTC 5 cut(s) 175, 1473, 1789, 1798, 3195
MmeI TCCRAC 3 cut(s) 1206, 1714, 1855
Mph1103I ATGCAT 2 cut(s) 1203, 2614
MroI TCCGGA 1 cut(s) 2051
MroNI GCCGGC 1 cut(s) 3016
MroXI GAANNNNTTC 6 cut(s) 319, 531, 801, 1233, 1327, 2754
MslI CAYNNNNRTG 3 cut(s) 314, 1346, 2805
MspA1I CMGCKG 1 cut(s) 690
MspCI CTTAAG 1 cut(s) 1504
MspI CCGG 4 cut(s) 2052, 2909, 3017, 3161
MspR9I CCNGG 6 cut(s) 214, 702, 1260, 2772, 3047, 3162
MunI CAATTG 2 cut(s) 1941, 2656
Mva1269I GAATGC 2 cut(s) 2475, 2717
MvaI CCWGG 5 cut(s) 214, 702, 1260, 2772, 3047
MvnI CGCG 3 cut(s) 278, 327, 2463
MwoI GCNNNNNNNGC 8 cut(s) 170, 402, 1207, 2491, 2879, 3211, 3321, 3330
NaeI GCCGGC 1 cut(s) 3018
NciI CCSGG 1 cut(s) 3162
NcoI CCATGG 1 cut(s) 391
NdeI CATATG 1 cut(s) 353
NgoMIV GCCGGC 1 cut(s) 3016
NlaIV GGNNCC 4 cut(s) 48, 65, 322, 389
NmeAIII GCCGAG 1 cut(s) 2046
NmuCI GTSAC 2 cut(s) 1099, 2428
NsiI ATGCAT 2 cut(s) 1203, 2614
NspI RCATGY 2 cut(s) 400, 3337
PaeI GCATGC 2 cut(s) 400, 3337
PaeR7I CTCGAG 1 cut(s) 426
PceI AGGCCT 1 cut(s) 1452
PctI GAATGC 2 cut(s) 2475, 2717
PdiI GCCGGC 1 cut(s) 3018
PdmI GAANNNNTTC 6 cut(s) 319, 531, 801, 1233, 1327, 2754
PflMI CCANNNNNTGG 2 cut(s) 1342, 2911
PfoI TCCNGGA 1 cut(s) 2770
PkrI GCNGC 6 cut(s) 63, 175, 592, 689, 943, 3323
PleI GAGTC 5 cut(s) 175, 1473, 1788, 1797, 3194
PpsI GAGTC 5 cut(s) 175, 1473, 1788, 1797, 3194
PshAI GACNNNNGTC 1 cut(s) 185
Psp124BI GAGCTC 1 cut(s) 87
Psp6I CCWGG 5 cut(s) 212, 700, 1258, 2770, 3045
PspEI GGTNACC 1 cut(s) 2428
PspFI CCCAGC 4 cut(s) 10, 204, 360, 1224
PspGI CCWGG 5 cut(s) 212, 700, 1258, 2770, 3045
PspN4I GGNNCC 4 cut(s) 48, 65, 322, 389
PspPI GGNCC 4 cut(s) 13, 698, 1438, 3158
PspXI VCTCGAGB 1 cut(s) 426
PstI CTGCAG 1 cut(s) 1988
PstNI CAGNNNCTG 1 cut(s) 590
PsuI RGATCY 8 cut(s) 46, 387, 859, 1163, 1508, 1690, 2038, 2213
PvuII CAGCTG 1 cut(s) 690
RsaI GTAC 5 cut(s) 2011, 2587, 2841, 2892, 3103
RsaNI GTAC 5 cut(s) 2010, 2586, 2840, 2891, 3102
RseI CAYNNNNRTG 3 cut(s) 314, 1346, 2805
SacI GAGCTC 1 cut(s) 87
SatI GCNGC 6 cut(s) 62, 174, 591, 688, 942, 3322
Sau96I GGNCC 4 cut(s) 13, 698, 1438, 3158
SchI GAGTC 5 cut(s) 175, 1473, 1789, 1798, 3195
ScrFI CCNGG 6 cut(s) 214, 702, 1260, 2772, 3047, 3162
SduI GDGCHC 2 cut(s) 87, 2904
SfaNI GCATC 8 cut(s) 199, 751, 1188, 2045, 2186, 2599, 2621, 2675
SfcI CTRYAG 1 cut(s) 1984
Sfr274I CTCGAG 1 cut(s) 426
SinI GGWCC 2 cut(s) 698, 1438
SlaI CTCGAG 1 cut(s) 426
SmiMI CAYNNNNRTG 3 cut(s) 314, 1346, 2805
SmlI CTYRAG 7 cut(s) 426, 1474, 1504, 1679, 1784, 2495, 3134
SmoI CTYRAG 7 cut(s) 426, 1474, 1504, 1679, 1784, 2495, 3134
SphI GCATGC 2 cut(s) 400, 3337
SseBI AGGCCT 1 cut(s) 1452
SsiI CCGC 9 cut(s) 61, 276, 325, 400, 607, 941, 2323, 3183, 3266
SspI AATATT 2 cut(s) 1053, 2782
SspMI CTAG 4 cut(s) 512, 708, 1112, 2564
SstI GAGCTC 1 cut(s) 87
StuI AGGCCT 1 cut(s) 1452
StyD4I CCNGG 6 cut(s) 212, 700, 1258, 2770, 3045, 3160
StyI CCWWGG 2 cut(s) 391, 1336
TaaI ACNGT 4 cut(s) 791, 2102, 2855, 3122
TaiI ACGT 4 cut(s) 1008, 1270, 2121, 2357
TaqI TCGA 6 cut(s) 223, 427, 1196, 1485, 2479, 2982
TaqII GACCGA 1 cut(s) 477
TatI WGTACW 3 cut(s) 2585, 2839, 2890
TauI GCSGC 2 cut(s) 64, 944
TscAI CASTG 4 cut(s) 2598, 3037, 3316, 3322
TseFI GTSAC 2 cut(s) 1099, 2428
TseI GCWGC 4 cut(s) 173, 590, 687, 3321
Tsp45I GTSAC 2 cut(s) 1099, 2428
TspGWI ACGGA 5 cut(s) 888, 1335, 1720, 1988, 3179
TspRI CASTG 4 cut(s) 2598, 3037, 3316, 3322
Van91I CCANNNNNTGG 2 cut(s) 1342, 2911
Vha464I CTTAAG 1 cut(s) 1504
VpaK11BI GGWCC 2 cut(s) 698, 1438
XapI RAATTY 7 cut(s) 444, 1399, 1597, 2405, 2617, 2933, 3038
XceI RCATGY 2 cut(s) 400, 3337
XcmI CCANNNNNNNNNTGG 2 cut(s) 1730, 2807
XhoI CTCGAG 1 cut(s) 426
XmiI GTMKAC 2 cut(s) 281, 2560
XmnI GAANNNNTTC 6 cut(s) 319, 531, 801, 1233, 1327, 2754
XspI CTAG 4 cut(s) 512, 708, 1112, 2564
Zsp2I ATGCAT 2 cut(s) 1203, 2614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.