Rw0G020500

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00926
OG0000105
Physical Location & Seq
Reverse (-)
67774 .. 72034
4261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G020500.1

Sequence Viewer

Length: 3609 bp
ATGGCGGCAACCTCTTCTCTCCGTCGATGGAAGTACGATGTGTTTCTAAGTTTCAGAGGGCCAGATACGCGCAAGGGGATTACCTCCGAGCTGTATCATAGACTAGAACGGAGAGGAATTAAAACCTTTATGGATGACCCCGATCTTCAGGTAGGAGATTCCGTTAGCCCTGAACTTTTAGCTGCAATTGAGGAGTCAAGATTTGCCATTGTCATGCTTTCACCCACCTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGACTATTCAATGCATGAAAGAGACGGGGCTACGAGTCATGCCCGTCTTCTACAACGTGGGGCCTTGCGATGTGCGGCACCAAAGGGGAAGTTTTGCGCTCAAAAGGAAACCCCGACACCAAGCAGACGTGGAAGTAAGGGAACATGAAGAAGCTTATGGCAAGACTGAGGATAAATTAAAGGCGTGGAGAGCTGCTTTGAAAGAAGTAGCCGATCTTTCTGGGTGGGATTCAAACAAGTTTAGAACTGATAGAGAACTTGTTGAGGCAATTGTTGGGACAATTGAGAATAAGGTGGTGTATAAATCTTCAAGTGTTGACAAGGGCTTAGTAGGAATGGATTTCCGTGTTGATGATTTATTACATAATTACATATATCAAGAGTTGGATGAGGTACGTATTATAGGGATACATGGGATGCGAGGCGTAGGTAAGACAACGTTTGCTCGGGCTTTTTATGATGACTTTTGTCAGGATTTTGAGCATAGATGCTTTCTTCCCAATGTTAGAGAAAGGTTTAAAGGGGGTGGCGTAGTTTCTTTACAAAAGAAGCTTCTTTCCAGCATCTTGATGGACAAAATTGAATACATAGAGGATGAATATGCAGGAGCTGCTATGATTCAAAGTAGTTTATGTAAAACAAAGGTGCTTGCTGTTATTGATGATGTGGATCAGTTGGCACAATTAGAAAAATTGGCTGGAAGTCGCGACTGGTTCGGTCCAGGGAGCAGAATTATCATAACCACCACGGATAATCACCTGTTAGAGGCACATGGTGTTGATGCTACATACAACGCTACTAGCTTAAGTCCTGATGAAGCTCTTGAACTCTTGAGTTTAAAGGCTTTCAAGAAAAGCTCCCCACCAGAAAATTATTCGGACCTGTGTGACGATATTTTAGGGTATGCTCAGGGGCTGCCGTTAGCTCTTGTGGTTATAGGTTCTTTTCTATGTGGTAGAAGCACCAATGAATGGGCAAGTGCAATTGACAGGCTAAAAAATACGCCAAATAGAGGAATTATGGATGTGCTTCAGATTAGTTTTGATGGACTAGAGGACATAGACAGAGAAATTTTCCTACATATTGCTTGTTTTTACAAGCGGAAAGATAAGGATCGTGTGACACAAATACTAGACTATTGTGAGCTGAACCCTGTCATTGGATTAAGAATTCTTGCTGATAAATCTCTCATTACTATTTCCAGCAACAAACTGTCAATGCATGATTTGCTACAAGAACTGGGCTGGGAAATAGTTCGTCGACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGAAATCCACAATATGTTGAAGAAAAATAAGGGAACAGAAGCAATCCAAGGCATGGTAATGGAGTTGCCTGAATTAGAAGTGGCTAATTGGAATCCAGAAGCCTTCTCAAATATTTCTCAACTTAGTCTTCTCCATATTCATAATGTGGACCTTCCCAATGGCCTCACTTGTCTTCCTAGTTCCTTGAGACTCCTGGAATGGCCTGGGTGTCCGTTAAGATCTCTCCCACGAAAGTTCGAACCAGATGAACTTATTGAACTTAACTTGTGTCACAGCAATATTGAAAATCTTTGGAAGGGAACAAAGAATTTTAAGAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTTGCAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTAAGAGTTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTACTGTCCTGAATCTTAAAGACTGCAAAAGTCTTGAGAATCTGCCAGATAGAATTGAAATAGAATCTCTTGAAAAATTTATTCTTTCTAACTGCTCAAAAATTAAAAAGATTCCCGAGTTTGATGGAAATATGGAGTGTTTGTCTGTGCTTCATCTCGACGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGGACGCCTGAGTAGCCTTGTGTCATTGAATCTAAGTAACTGCAAAAATCTTGTTTGTCTTCCAAGCACCATCAATAATTTGAAGTTTGTTGAAAATCTTGATCTTTCTGGATGCATGAAACTTGGCAAACATCAGGAAAGCGTGGGGGAGATGGACTGTTATGAGGAAAATGATTTGCATAGTGGGTCTGCAATAGAAATGTCATCTACCCATGATCTCATAAAGTATGTGAGAGGTTCAATCTTTCATGGATGCGAAGTAGTTTGGGGAGCTCTAAATAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAACACAGAACCCATGAGTTTCTACTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGATCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTATGTGGAAACAATTTTGTTCGTCTTCCTTCAGGCATTAGATTGCTTTCTAAGCTTGAGAACTTTAACGTGCAGAATTGCAGGAGACTTGAAGAGTTGTCAGACCTTCCATCAAATAGTATCCTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATATTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCATCAATATAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAAGAGGGATACTTTTCGAATTGTAATTCCTGGAAGTAATATCCCTGAGTGGTTCAGCCATCGAAGTGTTGGTTGTTCATTAAGTTTAGAGCTGCCTCCAGATTGGGATAGCCGTAGATTTTTGGGATTTGCTTTATGCGTTGTTTTTGAACTCGATGAGCACCATCAGGTTGATGAGCTTGATATTTTTCAATTCGAGAATTTTAACGCTACTCATCATCTTGCATGTTGTCTGAGGCTCAATGGAAGAGAGGCAGAAGCATGCAGAATAGTGCCTGCATTTCGCTTCAGTGAAGAATTTGGCCAGGTTGAGTCAGATCACCTGTGGCTATTCTATGCATCTCGTGATGCATTCTCTGTTGATGAATTCTCTGTTGGTCAAGAGTGGTCGCATAACAGTTGGAGTCAGCTTGATTTCTTATTTAAAACCACAGGCCTGGGTTTGAAGGTGAAGGAGTCTGGAGTCCGTCTGATATATGAGCAAGATGTGCAAGAGTTGAAGCAAACAACCACTCAATCAAGCAGTATGATGTCTACATATGAGGATATATTGATTGATTTTGACATTCCAGTTGAAGGGCAAACCAGTGGCACTGGTAGCGGAACTTGCACACTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1202

Amino Acids

135.95

Weight (kDa)

5.41

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 199 6.8e-47 TIR domain
TIR_2 PF13676 14 - 113 7.4e-17 TIR domain
NB-ARC PF00931 217 - 374 7.7e-17 NB-ARC domain
WHD_ROQ1 PF23282 442 - 511 3.4e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 609 - 627 6.1e-06 Leucine Rich Repeat
LRR_13 PF23286 700 - 785 2.6e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 711 - 781 3.3e-09 Leucine-rich repeat region
C-JID PF20160 1003 - 1158 9.2e-19 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 2 cut(s) 1236, 1627
AccI GTMKAC 2 cut(s) 1525, 3515
AccII CGCG 2 cut(s) 70, 972
AciI CCGC 4 cut(s) 5, 340, 1366, 3582
AclI AACGTT 1 cut(s) 704
AclWI GGATC 3 cut(s) 942, 1386, 2638
AcoI YGGCCR 1 cut(s) 3283
AcsI RAATTY 9 cut(s) 1335, 1434, 1882, 2111, 2645, 2857, 3181, 3278, 3347
AcuI CTGAAG 5 cut(s) 131, 1280, 2694, 2972, 3253
AcyI GRCGYC 1 cut(s) 2233
AdeI CACNNNGTG 1 cut(s) 1040
AfaI GTAC 3 cut(s) 35, 660, 2827
AfiI CCNNNNNNNGG 7 cut(s) 1030, 1236, 1277, 1424, 1627, 2576, 3557
AflII CTTAAG 1 cut(s) 1069
AjiI CACGTC 1 cut(s) 394
AjnI CCWGG 7 cut(s) 985, 1543, 1767, 1777, 3010, 3285, 3417
AjuI GAANNNNNNNTTGG 2 cut(s) 3339, 3371
Alw21I GWGCWC 2 cut(s) 2503, 3144
Alw26I GTCTC 7 cut(s) 281, 1536, 1572, 1756, 1928, 2192, 2758
AlwI GGATC 3 cut(s) 942, 1386, 2638
AlwNI CAGNNNCTG 2 cut(s) 875, 1180
Ama87I CYCGRG 2 cut(s) 711, 2150
AoxI GGCC 6 cut(s) 59, 326, 1735, 1775, 3283, 3415
ApeKI GCWGC 5 cut(s) 182, 458, 875, 1180, 3073
ApoI RAATTY 9 cut(s) 1335, 1434, 1882, 2111, 2645, 2857, 3181, 3278, 3347
Asp700I GAANNNNTTC 2 cut(s) 1518, 2957
AspLEI GCGC 2 cut(s) 72, 364
AspS9I GGNCC 5 cut(s) 59, 326, 983, 1144, 1723
AsuHPI GGTGA 6 cut(s) 213, 1013, 2636, 2680, 3293, 3442
AsuII TTCGAA 2 cut(s) 1812, 2998
AvaI CYCGRG 2 cut(s) 711, 2150
AvaII GGWCC 3 cut(s) 983, 1144, 1723
BalI TGGCCA 1 cut(s) 3285
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 2503
BauI CACGAG 1 cut(s) 3324
BbsI GAAGAC 6 cut(s) 304, 1694, 1739, 2279, 2696, 2960
Bbv12I GWGCWC 2 cut(s) 2503, 3144
BbvI GCAGC 5 cut(s) 169, 445, 862, 1167, 3060
BceAI ACGGC 2 cut(s) 1168, 3078
BcgI CGANNNNNNTGC 2 cut(s) 2183, 2217
BciT130I CCWGG 7 cut(s) 987, 1545, 1769, 1779, 3012, 3287, 3419
BciVI GTATCC 3 cut(s) 666, 2810, 2983
BcoDI GTCTC 7 cut(s) 281, 1536, 1572, 1756, 1928, 2192, 2758
BfaI CTAG 6 cut(s) 104, 1065, 1316, 1397, 1752, 2804
BfrI CTTAAG 1 cut(s) 1069
BfuI GTATCC 3 cut(s) 666, 2810, 2983
BglII AGATCT 2 cut(s) 1793, 1975
BisI GCNGC 7 cut(s) 6, 183, 341, 459, 876, 1181, 3074
BlsI GCNGC 7 cut(s) 7, 184, 342, 460, 877, 1182, 3075
Bme1390I CCNGG 7 cut(s) 987, 1545, 1769, 1779, 3012, 3287, 3419
Bme18I GGWCC 3 cut(s) 983, 1144, 1723
BmeT110I CYCGRG 2 cut(s) 711, 2150
BmgBI CACGTC 1 cut(s) 394
BmgT120I GGNCC 5 cut(s) 59, 326, 983, 1144, 1723
BmiI GGNNCC 2 cut(s) 327, 344
BmrFI CCNGG 7 cut(s) 987, 1545, 1769, 1779, 3012, 3287, 3419
BmrI ACTGGG 1 cut(s) 1514
BmuI ACTGGG 1 cut(s) 1514
BoxI GACNNNNGTC 1 cut(s) 732
BpiI GAAGAC 6 cut(s) 304, 1694, 1739, 2279, 2696, 2960
BpmI CTGGAG 2 cut(s) 3063, 3462
Bpu10I CCTNAGC 1 cut(s) 1173
Bpu14I TTCGAA 2 cut(s) 1812, 2998
BpuEI CTTGAG 5 cut(s) 1117, 1780, 1985, 2090, 2756
BsaAI YACGTR 1 cut(s) 662
BsaBI GATNNNNATC 2 cut(s) 933, 1377
BsaHI GRCGYC 1 cut(s) 2233
BsaI GGTCTC 2 cut(s) 1572, 1928
BsaJI CCNNGG 6 cut(s) 986, 1011, 1621, 1778, 2017, 3418
BsaXI ACNNNNNCTCC 4 cut(s) 147, 177, 2162, 2192
Bsc4I CCNNNNNNNGG 7 cut(s) 1030, 1236, 1277, 1424, 1627, 2576, 3557
Bse1I ACTGG 5 cut(s) 980, 1509, 3551, 3567, 3580
Bse8I GATNNNNATC 2 cut(s) 933, 1377
BseBI CCWGG 7 cut(s) 987, 1545, 1769, 1779, 3012, 3287, 3419
BseDI CCNNGG 6 cut(s) 986, 1011, 1621, 1778, 2017, 3418
BseJI GATNNNNATC 2 cut(s) 933, 1377
BseLI CCNNNNNNNGG 7 cut(s) 1030, 1236, 1277, 1424, 1627, 2576, 3557
BseMII CTCAG 6 cut(s) 423, 1187, 2228, 2597, 3018, 3206
BseNI ACTGG 5 cut(s) 980, 1509, 3551, 3567, 3580
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 5 cut(s) 169, 445, 862, 1167, 3060
BseYI CCCAGC 1 cut(s) 1509
BsgI GTGCAG 1 cut(s) 2771
Bsh1236I CGCG 2 cut(s) 70, 972
BshFI GGCC 6 cut(s) 61, 328, 1737, 1777, 3285, 3417
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 2 cut(s) 2503, 3144
BsiHKCI CYCGRG 2 cut(s) 711, 2150
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 7 cut(s) 1030, 1236, 1277, 1424, 1627, 2576, 3557
BsmAI GTCTC 7 cut(s) 281, 1536, 1572, 1756, 1928, 2192, 2758
BsmBI CGTCTC 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 556
BsmI GAATGC 1 cut(s) 3332
BsnI GGCC 6 cut(s) 61, 328, 1737, 1777, 3285, 3417
Bso31I GGTCTC 2 cut(s) 1572, 1928
BsoBI CYCGRG 2 cut(s) 711, 2150
Bsp119I TTCGAA 2 cut(s) 1812, 2998
Bsp1286I GDGCHC 2 cut(s) 2503, 3144
Bsp68I TCGCGA 1 cut(s) 972
BspACI CCGC 4 cut(s) 5, 340, 1366, 3582
BspANI GGCC 6 cut(s) 61, 328, 1737, 1777, 3285, 3417
BspCNI CTCAG 6 cut(s) 424, 1186, 2229, 2598, 3019, 3207
BspFNI CGCG 2 cut(s) 70, 972
BspHI TCATGA 1 cut(s) 1570
BspLI GGNNCC 2 cut(s) 327, 344
BspPI GGATC 3 cut(s) 942, 1386, 2638
BspT104I TTCGAA 2 cut(s) 1812, 2998
BspT107I GGYRCC 1 cut(s) 342
BspTI CTTAAG 1 cut(s) 1069
BspTNI GGTCTC 2 cut(s) 1572, 1928
BsrI ACTGG 5 cut(s) 980, 1509, 3551, 3567, 3580
BssECI CCNNGG 6 cut(s) 986, 1011, 1621, 1778, 2017, 3418
BssNI GRCGYC 1 cut(s) 2233
BssSI CACGAG 1 cut(s) 3324
BssT1I CCWWGG 2 cut(s) 1621, 2017
Bst2BI CACGAG 1 cut(s) 3324
Bst2UI CCWGG 7 cut(s) 987, 1545, 1769, 1779, 3012, 3287, 3419
Bst4CI ACNGT 5 cut(s) 1479, 1530, 2041, 2387, 3380
Bst6I CTCTTC 3 cut(s) 19, 2766, 3223
BstACI GRCGYC 1 cut(s) 2233
BstAFI CTTAAG 1 cut(s) 1069
BstAPI GCANNNNNTGC 3 cut(s) 875, 1492, 3469
BstBAI YACGTR 1 cut(s) 662
BstBI TTCGAA 2 cut(s) 1812, 2998
BstC8I GCNNGC 4 cut(s) 915, 1951, 3244, 3258
BstDSI CCRYGG 1 cut(s) 1011
BstEII GGTNACC 1 cut(s) 2668
BstENI CCTNNNNNAGG 1 cut(s) 1028
BstFNI CGCG 2 cut(s) 70, 972
BstHHI GCGC 2 cut(s) 72, 364
BstMAI GTCTC 7 cut(s) 281, 1536, 1572, 1756, 1928, 2192, 2758
BstMWI GCNNNNNNNGC 9 cut(s) 67, 455, 875, 1492, 2241, 2731, 3469, 3579, 3588
BstNI CCWGG 7 cut(s) 987, 1545, 1769, 1779, 3012, 3287, 3419
BstNSI RCATGY 2 cut(s) 3210, 3246
BstPAI GACNNNNGTC 1 cut(s) 732
BstPI GGTNACC 1 cut(s) 2668
BstSCI CCNGG 7 cut(s) 985, 1543, 1767, 1777, 3010, 3285, 3417
BstSNI TACGTA 1 cut(s) 662
BstUI CGCG 2 cut(s) 70, 972
BstV1I GCAGC 5 cut(s) 169, 445, 862, 1167, 3060
BstV2I GAAGAC 6 cut(s) 304, 1694, 1739, 2279, 2696, 2960
BstX2I RGATCY 2 cut(s) 1793, 1975
BstXI CCANNNNNNTGG 1 cut(s) 3418
BstYI RGATCY 2 cut(s) 1793, 1975
BsuI GTATCC 3 cut(s) 666, 2810, 2983
BsuRI GGCC 6 cut(s) 61, 328, 1737, 1777, 3285, 3417
BtgI CCRYGG 1 cut(s) 1011
BtgZI GCGATG 1 cut(s) 348
BtrI CACGTC 1 cut(s) 394
BtsIMutI CAGTG 4 cut(s) 2831, 3277, 3573, 3574
BtuMI TCGCGA 1 cut(s) 972
Cac8I GCNNGC 4 cut(s) 915, 1951, 3244, 3258
CaiI CAGNNNCTG 2 cut(s) 875, 1180
CciI TCATGA 1 cut(s) 1570
CfoI GCGC 2 cut(s) 72, 364
Cfr13I GGNCC 5 cut(s) 59, 326, 983, 1144, 1723
CseI GACGC 1 cut(s) 2241
Csp6I GTAC 3 cut(s) 34, 659, 2826
CviQI GTAC 3 cut(s) 34, 659, 2826
DraI TTTAAA 3 cut(s) 784, 1104, 3406
DraIII CACNNNGTG 1 cut(s) 1040
EaeI YGGCCR 1 cut(s) 3283
Eam1104I CTCTTC 3 cut(s) 19, 2766, 3223
EarI CTCTTC 3 cut(s) 19, 2766, 3223
Ecl136II GAGCTC 1 cut(s) 2501
Eco105I TACGTA 1 cut(s) 662
Eco130I CCWWGG 2 cut(s) 1621, 2017
Eco147I AGGCCT 1 cut(s) 3417
Eco24I GRGCYC 1 cut(s) 2503
Eco31I GGTCTC 2 cut(s) 1572, 1928
Eco47I GGWCC 3 cut(s) 983, 1144, 1723
Eco53kI GAGCTC 1 cut(s) 2501
Eco57I CTGAAG 5 cut(s) 131, 1280, 2694, 2972, 3253
Eco88I CYCGRG 2 cut(s) 711, 2150
Eco91I GGTNACC 1 cut(s) 2668
EcoICRI GAGCTC 1 cut(s) 2501
EcoNI CCTNNNNNAGG 1 cut(s) 1028
EcoO109I RGGNCCY 1 cut(s) 326
EcoO65I GGTNACC 1 cut(s) 2668
EcoRI GAATTC 2 cut(s) 1434, 3347
EcoRII CCWGG 7 cut(s) 985, 1543, 1767, 1777, 3010, 3285, 3417
EcoT14I CCWWGG 2 cut(s) 1621, 2017
EcoT22I ATGCAT 6 cut(s) 281, 1488, 2345, 2854, 3322, 3334
EcoT38I GRGCYC 1 cut(s) 2503
ErhI CCWWGG 2 cut(s) 1621, 2017
Esp3I CGTCTC 1 cut(s) 281
FaqI GGGAC 1 cut(s) 556
FauNDI CATATG 1 cut(s) 3520
FblI GTMKAC 2 cut(s) 1525, 3515
Fnu4HI GCNGC 7 cut(s) 6, 183, 341, 459, 876, 1181, 3074
FriOI GRGCYC 1 cut(s) 2503
Fsp4HI GCNGC 7 cut(s) 6, 183, 341, 459, 876, 1181, 3074
FspBI CTAG 6 cut(s) 104, 1065, 1316, 1397, 1752, 2804
GlaI GCGC 2 cut(s) 71, 363
GluI GCNGC 7 cut(s) 6, 183, 341, 459, 876, 1181, 3074
GsaI CCCAGC 1 cut(s) 1513
GsuI CTGGAG 2 cut(s) 3063, 3462
HaeIII GGCC 6 cut(s) 61, 328, 1737, 1777, 3285, 3417
HgaI GACGC 1 cut(s) 2241
HhaI GCGC 2 cut(s) 72, 364
Hin1I GRCGYC 1 cut(s) 2233
Hin6I GCGC 2 cut(s) 70, 362
HinP1I GCGC 2 cut(s) 70, 362
HincII GTYRAC 2 cut(s) 583, 1526
HindII GTYRAC 2 cut(s) 583, 1526
HindIII AAGCTT 4 cut(s) 417, 815, 2033, 2732
HphI GGTGA 6 cut(s) 213, 1013, 2636, 2680, 3293, 3442
Hpy166II GTNNAC 5 cut(s) 583, 1526, 1723, 2542, 3516
Hpy8I GTNNAC 5 cut(s) 583, 1526, 1723, 2542, 3516
Hpy99I CGWCG 3 cut(s) 27, 1527, 2198
HpyCH4III ACNGT 5 cut(s) 1479, 1530, 2041, 2387, 3380
HpyCH4IV ACGT 6 cut(s) 321, 393, 661, 704, 1552, 2748
HpyF10VI GCNNNNNNNGC 9 cut(s) 67, 455, 875, 1492, 2241, 2731, 3469, 3579, 3588
HpySE526I ACGT 6 cut(s) 321, 393, 661, 704, 1552, 2748
Hsp92I GRCGYC 1 cut(s) 2233
HspAI GCGC 2 cut(s) 70, 362
LmnI GCTCC 4 cut(s) 872, 990, 1127, 2498
Lsp1109I GCAGC 5 cut(s) 169, 445, 862, 1167, 3060
MaeI CTAG 6 cut(s) 104, 1065, 1316, 1397, 1752, 2804
MaeII ACGT 6 cut(s) 321, 393, 661, 704, 1552, 2748
MaeIII GTNAC 6 cut(s) 1151, 1384, 1844, 2264, 2651, 2668
MfeI CAATTG 6 cut(s) 186, 534, 546, 1248, 2226, 2896
MflI RGATCY 2 cut(s) 1793, 1975
MhlI GDGCHC 2 cut(s) 2503, 3144
MlsI TGGCCA 1 cut(s) 3285
MluNI TGGCCA 1 cut(s) 3285
MlyI GAGTC 7 cut(s) 203, 309, 1758, 3302, 3394, 3446, 3453
MmeI TCCRAC 2 cut(s) 630, 3362
Mox20I TGGCCA 1 cut(s) 3285
Mph1103I ATGCAT 6 cut(s) 281, 1488, 2345, 2854, 3322, 3334
MroXI GAANNNNTTC 2 cut(s) 1518, 2957
MscI TGGCCA 1 cut(s) 3285
MslI CAYNNNNRTG 4 cut(s) 212, 833, 1631, 3045
Msp20I TGGCCA 1 cut(s) 3285
MspCI CTTAAG 1 cut(s) 1069
MspR9I CCNGG 7 cut(s) 987, 1545, 1769, 1779, 3012, 3287, 3419
MunI CAATTG 6 cut(s) 186, 534, 546, 1248, 2226, 2896
Mva1269I GAATGC 1 cut(s) 3332
MvaI CCWGG 7 cut(s) 987, 1545, 1769, 1779, 3012, 3287, 3419
MvnI CGCG 2 cut(s) 70, 972
MwoI GCNNNNNNNGC 9 cut(s) 67, 455, 875, 1492, 2241, 2731, 3469, 3579, 3588
NdeI CATATG 1 cut(s) 3520
NlaIV GGNNCC 2 cut(s) 327, 344
NmuCI GTSAC 4 cut(s) 1151, 1384, 1844, 2668
NruI TCGCGA 1 cut(s) 972
NsiI ATGCAT 6 cut(s) 281, 1488, 2345, 2854, 3322, 3334
NspI RCATGY 2 cut(s) 3210, 3246
NspV TTCGAA 2 cut(s) 1812, 2998
PaeI GCATGC 1 cut(s) 3246
PagI TCATGA 1 cut(s) 1570
PceI AGGCCT 1 cut(s) 3417
PctI GAATGC 1 cut(s) 3332
PdmI GAANNNNTTC 2 cut(s) 1518, 2957
PflMI CCANNNNNTGG 2 cut(s) 1236, 1627
PfoI TCCNGGA 2 cut(s) 1767, 3010
PkrI GCNGC 7 cut(s) 7, 184, 342, 460, 877, 1182, 3075
PleI GAGTC 7 cut(s) 202, 308, 1758, 3301, 3393, 3445, 3452
PpsI GAGTC 7 cut(s) 202, 308, 1758, 3301, 3393, 3445, 3452
Ppu21I YACGTR 1 cut(s) 662
PshAI GACNNNNGTC 1 cut(s) 732
Psp124BI GAGCTC 1 cut(s) 2503
Psp1406I AACGTT 1 cut(s) 704
Psp6I CCWGG 7 cut(s) 985, 1543, 1767, 1777, 3010, 3285, 3417
PspEI GGTNACC 1 cut(s) 2668
PspFI CCCAGC 1 cut(s) 1509
PspGI CCWGG 7 cut(s) 985, 1543, 1767, 1777, 3010, 3285, 3417
PspN4I GGNNCC 2 cut(s) 327, 344
PspPI GGNCC 5 cut(s) 59, 326, 983, 1144, 1723
PstNI CAGNNNCTG 2 cut(s) 875, 1180
PsuI RGATCY 2 cut(s) 1793, 1975
RruI TCGCGA 1 cut(s) 972
RsaI GTAC 3 cut(s) 35, 660, 2827
RsaNI GTAC 3 cut(s) 34, 659, 2826
RseI CAYNNNNRTG 4 cut(s) 212, 833, 1631, 3045
SacI GAGCTC 1 cut(s) 2503
SalI GTCGAC 1 cut(s) 1524
SatI GCNGC 7 cut(s) 6, 183, 341, 459, 876, 1181, 3074
Sau96I GGNCC 5 cut(s) 59, 326, 983, 1144, 1723
SchI GAGTC 7 cut(s) 203, 309, 1758, 3302, 3394, 3446, 3453
ScrFI CCNGG 7 cut(s) 987, 1545, 1769, 1779, 3012, 3287, 3419
SduI GDGCHC 2 cut(s) 2503, 3144
SfuI TTCGAA 2 cut(s) 1812, 2998
SinI GGWCC 3 cut(s) 983, 1144, 1723
SmiMI CAYNNNNRTG 4 cut(s) 212, 833, 1631, 3045
SmlI CTYRAG 6 cut(s) 1069, 1096, 1759, 1964, 2069, 2735
SmoI CTYRAG 6 cut(s) 1069, 1096, 1759, 1964, 2069, 2735
SnaBI TACGTA 1 cut(s) 662
SphI GCATGC 1 cut(s) 3246
SseBI AGGCCT 1 cut(s) 3417
SsiI CCGC 4 cut(s) 5, 340, 1366, 3582
SspI AATATT 3 cut(s) 1687, 1855, 2840
SspMI CTAG 6 cut(s) 104, 1065, 1316, 1397, 1752, 2804
SstI GAGCTC 1 cut(s) 2503
StuI AGGCCT 1 cut(s) 3417
StyD4I CCNGG 7 cut(s) 985, 1543, 1767, 1777, 3010, 3285, 3417
StyI CCWWGG 2 cut(s) 1621, 2017
TaaI ACNGT 5 cut(s) 1479, 1530, 2041, 2387, 3380
TaiI ACGT 6 cut(s) 324, 396, 664, 707, 1555, 2751
TaqI TCGA 8 cut(s) 25, 1525, 1812, 2193, 2998, 3043, 3135, 3177
TaqII GACCGA 1 cut(s) 971
TatI WGTACW 1 cut(s) 2825
TauI GCSGC 2 cut(s) 8, 343
TscAI CASTG 4 cut(s) 2838, 3277, 3574, 3580
TseFI GTSAC 4 cut(s) 1151, 1384, 1844, 2668
TseI GCWGC 5 cut(s) 182, 458, 875, 1180, 3073
Tsp45I GTSAC 4 cut(s) 1151, 1384, 1844, 2668
TspGWI ACGGA 7 cut(s) 11, 124, 151, 599, 1028, 1776, 3437
TspRI CASTG 4 cut(s) 2838, 3277, 3574, 3580
Van91I CCANNNNNTGG 2 cut(s) 1236, 1627
Vha464I CTTAAG 1 cut(s) 1069
VpaK11BI GGWCC 3 cut(s) 983, 1144, 1723
XagI CCTNNNNNAGG 1 cut(s) 1028
XapI RAATTY 9 cut(s) 1335, 1434, 1882, 2111, 2645, 2857, 3181, 3278, 3347
XceI RCATGY 2 cut(s) 3210, 3246
XcmI CCANNNNNNNNNTGG 3 cut(s) 832, 2015, 3047
XmiI GTMKAC 2 cut(s) 1525, 3515
XmnI GAANNNNTTC 2 cut(s) 1518, 2957
XspI CTAG 6 cut(s) 104, 1065, 1316, 1397, 1752, 2804
Zsp2I ATGCAT 6 cut(s) 281, 1488, 2345, 2854, 3322, 3334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.