MD12G1125200.v1.1

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000105
Physical Location & Seq
Reverse (-)
20052936 .. 20058398
5463 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1125200.v1.1.491

Sequence Viewer

Length: 4227 bp
ATGGAGCTTGCTACGAATGCCGCTGCAGTGTCTTCTTCTTCGTCGTCTTCTTCTCAGGCCCGTCAATGGAAATACGAAGTTTTTCTAAGTTTCCGAGGGGAAGACACCCGCAAGAGTTTCGTCGGCCATCTGTACGCTGCATTACATAATAGAGGAATTCTGACCTTCAAAGATGACAAAAGGCTTGAGTATGGAAAATCCATTTCCACAGAACTTCTGCAAGCAATTGAGGAGTCAAAGCTTGCCCTCGTCATTCTTTCACCAAACTATGCCAATTCATCGTGGTGTTTGAATGAGCTTGTCAAGATTATGGAATGCAGAAAAGACATGGGGCAAGAAGTCGTGCCAATCTATCACAACGTGGATCCGTCTGAGGTGCGCCACCAAACGGGAAGGTTCTTGCTGGCTGTGAGGAATGTAACGGAGCATGAAGAAGTTTATTCTGAGGATAGGAACAAAATAAACCGGTGGAGGGCTGCTTTGAGAGAGGTGGCTGATCTTAAGGGCTGGGTTGTACAACATTATGTGGAGGCAGAACTTGTAGAATCTGTTGCCACATGGGTACTAAACCGTTCGTTGTATGCATCTTCAACTGTCGACGAGGGCTTGGTAGGAATGGAATCTCGCATTGATGAAATACTACAAGATCACATATGTGGATGGACAACTGACTGTGTACGCATTATAGGGATACATGGGATGCGAGGAATTGGAAAGACAACAGTTGCCAGAGCTGTTTATGATCAAATCTGTCACCACTATGAACGTAGCTGCTTTCTTTCCAATGTTAGATCTGAAACTTCTAAAAACGGTCTAGTTTCTTTACAAGAAAAACTTCTCTCCAAAACCCTGGTGGAAAAAATTGGAAACATAGAGGATGAGCATATAGGAGCTGCTATGATAAAAAAAAGGTTAGCGTTTCTCAAGGTGCTTCTTGTTGTTGATGATGTGGATCAATTAGCACAATTAGAAAAATTGGCTGGAGGTCGCGACTGGTTTGGTCTAGGGAGTAGAATTATCATAACGAGTACAGATGAACATTTGTTGAAGGTGCATGGTGTTGATGGAATATACAAGGTCACTGGCTTAGACCATGACGAGGCTCTTAAACTTTTGAGTTCAAAAGCGTTTAAGAAGGGCGATCACCGTGAAGATTATTTGAGTCTTTGCAGCGATATAGTACAGTATGCTGGTGGCCTTCCATTAGCTATCATTGTCGTAGGTTCCTTTCTATGTGGCAGAAGCACCCTCGAATGGAAAAGTGCCATAGATAGGCTGAGGAATACATCAGATAGAAGTATCATGGATGTTCTTCACATTAGTTTTGATGGACTAAGGGAAACAGAAAAAGAAATATTTCTACATATTGCATGCTTTTTTAAAGGGAAGGATAGGGTTCGTGTAACCGAGATACTAAACTATTGTAGGCTCTACCCTGAGATTGGGTTAACTATTCTTTCTGAGAAATCTCTTGTTACTTTTTCCGGCAACAAGTTGGAGATGCATGGTTTGCTACAAGAAATGGGATGGCAAATAGTTAGTCGAGAGTCGGCTACTGATCCAGGCAAACGTAGTAGGTTATGGTCTCATGAAGACATCCACAATGTGCTGACAAAAAATAAGGGAACAAACGTAGTTCAAGGGATAGTTATGGAGTTGCCTAAATTAGAAGTGGCTCATTGGAATCCAGAAGCATTCTCAAATTTGTCTCAACTTCGTTTTCTCCAAGTTCATAATGTGCATCCTTCCCAAGGCCTCACTTGTCTTCCGGAATCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTAAGATCTCTCCCACAATATTTCCAACCAGATGAGCTGATTGAACTTAACTTGTGCCACAGCAACCTTGATCAGCTTTGGAAGGGGATAAAGCGTTTTCCCAAGTTGAAGTTCATCAAAATCTGCCATTCTCACCACATTTCTGAGACCCCAGACTTCACAGGTGTTCAGGAACTTGAGAGTTTAGATCTTGAAGGATGTAAAAACTTGGTTGGAATTCATCAGTCTCTTGGACTTCTCAAAAAGCTGACCCTCCTAAATCTTAAAGATTGCGAAAGTCTCGAGAGTCTGCCAGGAAAAATTGAAATGGAGTCTCTTGAAACTTTTATTCTTTCTGGCTGCTTAAAAGTCAAGCAGGTTCCTGAGTTTGGAAATATGCAACTTCTGTCAGCGCTTAAACTCAATGAAACTGCCATAGAAACAATACCGGAGTCAATTGAACACCTTAGTGGCCTTGTTACATTAGATCTAAGCAACTGCAAGGATCTTGTTTGTCTTCCAAGCACCATTAACAGATTGAAGTCTCTGAAAAATCTTAATCTTTCTGGATGCTTGAAACTTGGCGAAGAGCAGGAAAGCATGTCGGAGGTGGAGTGTTTGGAGAAGATTGATGACAGTAGAACTGCTGAAACAGAAGTGCCTAATTTTGGAGTGATGCAAAAAGTGAAGAAAGAACCAGTGACTTTCTGGTCGCCTTCATTTGGTCTGTGTAGCTTAACAGATCTGAACCTAAGTAACTGTGAACTTCAGGAAGGAGCATTTCTCAATGGTCTTGGGGTCTTATCCTCACTAGTGGCATTGAATCTCAGTGGAAATAATTTTGTTAGTCTTCCATTAAGCATCCGATCCCTTGTTAAGCTTGAGAGCATTAACATCGAGAACTGCAAGAGACTTACAGAGCTGTCAGGTCTCCCATCAAATAGTAAATTGGATGTGAGGGCAGATGGTTGCTCGTCCCTTGAAATTTTCAATACATCCGAGCTCAATGTATTGGAGAAATCATATTTCAATTTCCTGAATTGCTTCAAACTAAATGACAACCAAAGTTTACTTAATATGACATTTGAAATGCTGAAGAAATTCCTTCATCGGTTGTGTGGAACCTCTAGTGCAAGAGAAACTTTCCAAATTGCAATTCCTGGAAGTGAAATTCCTAAGTGGTTCAGCCATCAAAACGATGTTGGGAGTTCATTAACCATAGACCTACCTCCACTTTGGAATAAAAGTAAGTTCATGGGATACGCTCTGTGTGCTGTTTTTGTACTAGACGAGCATTGTGTGGTTGATGAACACGATACATTTCAATTTAAAACTTTCCATGCTACACATCATCTTGTTTGTCGATTGAAACACAATGGAAAACAATTAGAAGTATGTGGCACACAGCCTGCCTTTCGCTTTAGCGAAAAGTTTTGCCAGGTTGATTCGGATCATCTTTGGATGTTCTATGTATCCCGTGATAAATACTTTGGTACGGACTGGCATAACAGTTGCAGTCAGATTGAGTTCTTGTTTGAAACCAGAGGGCCAGGCTTGAAGGTGAAGAAGTGCGGAGTACGGTTGATGTACGAGCAAGATGTGGGAGCTCTACAATCTGAAAGTGCCACTGTTAATACATCAATTTTTCCAGTTGAGGAGGATGTCATCAACATGATCGATGATAACGTGACACACACTTCCTCGGACATGACATTACATCAAAGGGAATGGCAACTTGTCCATTCTAACGGTTTTTCTCATTCTGTGGGCATAAATGAGGACATAACCAAACGTGCTTCATCGGACTCCTTTCTACAGTGGACACAATGGCAACTGCTGGATTCCATCCTCCCAACAGGCGGTTTTACTCATTCTTTTGGTCTCGAGGCTGCCATCCAAGCTCACGTAGTGTCTAGTCCCAAAGATCTACAAACCTTTATAATCCGTCTGTTGGAAAATACAGGAAGCTTGCTCCTTCCGTTTGTGTATTCTACAACAATATCACCTAATCTAGAGACCACTCGCAAACTCGATAAGATGTTGGATGCAATGCTTACCGATGAAGTGGTTAGAAAGGCGTCAATTTCAAAAGGCACGGAACTAATGAGGGTGGCTGCAGCTGTGTACGCAGAAATACCATCAGTTACATCTACGATGGAAGCTTGTTTAAGTTCCGGGGACGTTTCTTTTCACCATGCTCATGCATTTGGCCTTATATGTGGACTGCTAGGGTTGGATAGCACTACTACTCAAAGAGCGTACATGTTTATTACTATGAGAGATGTCATTTCTGCTGCAACAAGGCTCAATCTGGTAGGCCCGCTTGGTGCGGCTGTGCTGCAGCATAAGATTGCTTCCGGCGCCGCTGAAGCTATACTTAATCGGTGGAAGGACCGCCCAGTTGAGGAAGCATCGCAAGCTCTTTCTTTGCCCCAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1409

Amino Acids

157.93

Weight (kDa)

5.91

Isoelectric Point (pI)

43.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 194 1.7e-48 TIR domain
TIR_2 PF13676 27 - 123 1.9e-15 TIR domain
NB-ARC PF00931 225 - 379 5.9e-17 NB-ARC domain
WHD_ROQ1 PF23282 447 - 516 1.2e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 706 - 792 2.4e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 713 - 815 1.8e-09 Leucine-rich repeat region
C-JID PF20160 981 - 1129 4.1e-20 C-JID domain
UreF PF01730 1232 - 1378 7.4e-21 UreF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3725
AasI GACNNNNNNGTC 1 cut(s) 2494
Acc36I ACCTGC 1 cut(s) 2152
AccB1I GGYRCC 1 cut(s) 4145
AccI GTMKAC 1 cut(s) 597
AccII CGCG 1 cut(s) 990
AccIII TCCGGA 1 cut(s) 1768
AciI CCGC 8 cut(s) 21, 109, 3357, 3645, 4106, 4115, 4149, 4180
AclWI GGATC 7 cut(s) 359, 372, 960, 1553, 2298, 2646, 3243
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 7 cut(s) 156, 1702, 2022, 2769, 2885, 2955, 4221
AcuI CTGAAG 3 cut(s) 2537, 2900, 4173
AcyI GRCGYC 2 cut(s) 3863, 4146
AdeI CACNNNGTG 3 cut(s) 361, 1606, 3694
AfeI AGCGCT 1 cut(s) 2199
AflII CTTAAG 2 cut(s) 500, 1807
AflIII ACRYGT 1 cut(s) 4047
AgeI ACCGGT 1 cut(s) 465
AhlI ACTAGT 1 cut(s) 2596
AjnI CCWGG 6 cut(s) 849, 1561, 2098, 2944, 3222, 3334
AjuI GAANNNNNNNTTGG 4 cut(s) 1901, 1933, 2925, 2957
AleI CACNNNNGTG 2 cut(s) 654, 2253
Alw21I GWGCWC 2 cut(s) 2790, 3394
AlwI GGATC 7 cut(s) 359, 372, 960, 1553, 2298, 2646, 3243
Ama87I CYCGRG 2 cut(s) 2087, 3668
Aor13HI TCCGGA 1 cut(s) 1768
Aor51HI AGCGCT 1 cut(s) 2199
AoxI GGCC 8 cut(s) 57, 124, 1195, 1753, 2257, 3332, 3994, 4102
ApoI RAATTY 7 cut(s) 156, 1702, 2022, 2769, 2885, 2955, 4221
ArsI GACNNNNNNTTYG 2 cut(s) 1525, 1557
AsiGI ACCGGT 1 cut(s) 465
Asp700I GAANNNNTTC 5 cut(s) 81, 834, 1356, 2828, 2885
AspLEI GCGC 3 cut(s) 381, 2200, 4148
AspS9I GGNCC 4 cut(s) 58, 3332, 4103, 4177
AsuC2I CCSGG 1 cut(s) 3961
AsuHPI GGTGA 7 cut(s) 252, 746, 1136, 1931, 3358, 3780, 3968
AvaI CYCGRG 2 cut(s) 2087, 3668
AvaII GGWCC 1 cut(s) 4177
BamHI GGATCC 1 cut(s) 364
BanI GGYRCC 1 cut(s) 4145
BanII GRGCYC 2 cut(s) 2790, 3394
BbsI GAAGAC 7 cut(s) 24, 39, 108, 1599, 1757, 2294, 2627
Bbv12I GWGCWC 2 cut(s) 2790, 3394
BbvCI CCTCAGC 1 cut(s) 1277
BcgI CGANNNNNNTGC 4 cut(s) 100, 134, 2662, 2696
BciT130I CCWGG 6 cut(s) 851, 1563, 2100, 2946, 3224, 3336
BciVI GTATCC 4 cut(s) 684, 1812, 3038, 3268
BclI TGATCA 2 cut(s) 742, 1876
BcnI CCSGG 1 cut(s) 3961
BcuI ACTAGT 1 cut(s) 2596
BfaI CTAG 8 cut(s) 815, 1004, 2597, 2913, 3071, 3699, 3797, 4013
BfmI CTRYAG 4 cut(s) 24, 3599, 3900, 4124
BfoI RGCGCY 2 cut(s) 2201, 4149
BfrI CTTAAG 2 cut(s) 500, 1807
BfuAI ACCTGC 1 cut(s) 2152
BfuI GTATCC 4 cut(s) 684, 1812, 3038, 3268
BglII AGATCT 6 cut(s) 791, 1811, 1993, 2272, 2527, 3709
Bme1390I CCNGG 7 cut(s) 851, 1563, 2100, 2946, 3224, 3336, 3961
Bme18I GGWCC 1 cut(s) 4177
BmeT110I CYCGRG 2 cut(s) 2087, 3668
BmgT120I GGNCC 4 cut(s) 58, 3332, 4103, 4177
BmiI GGNNCC 5 cut(s) 366, 1225, 2166, 2908, 4147
BmrFI CCNGG 7 cut(s) 851, 1563, 2100, 2946, 3224, 3336, 3961
BmrI ACTGGG 2 cut(s) 1806, 4178
BmsI GCATC 9 cut(s) 593, 690, 1491, 1750, 2345, 2451, 2655, 3820, 4205
BmuI ACTGGG 2 cut(s) 1806, 4178
BpiI GAAGAC 7 cut(s) 24, 39, 108, 1599, 1757, 2294, 2627
BplI GAGNNNNNCTC 2 cut(s) 2553, 2585
BpmI CTGGAG 1 cut(s) 1002
Bpu10I CCTNAGC 1 cut(s) 1277
BpuEI CTTGAG 5 cut(s) 206, 908, 1798, 2003, 2687
BpuMI CCSGG 1 cut(s) 3961
Bsa29I ATCGAT 1 cut(s) 3462
BsaAI YACGTR 1 cut(s) 3691
BsaBI GATNNNNATC 2 cut(s) 951, 3234
BsaHI GRCGYC 2 cut(s) 3863, 4146
BsaI GGTCTC 5 cut(s) 1590, 1946, 2720, 3671, 3794
BsaJI CCNNGG 5 cut(s) 94, 849, 1750, 3486, 3960
BsaWI WCCGGW 3 cut(s) 465, 1768, 2233
BsaXI ACNNNNNCTCC 2 cut(s) 2384, 2414
Bse118I RCCGGY 1 cut(s) 465
Bse1I ACTGG 7 cut(s) 998, 1087, 1801, 2483, 3290, 3434, 4184
Bse3DI GCAATG 1 cut(s) 3840
Bse8I GATNNNNATC 2 cut(s) 951, 3234
BseAI TCCGGA 1 cut(s) 1768
BseBI CCWGG 6 cut(s) 851, 1563, 2100, 2946, 3224, 3336
BseCI ATCGAT 1 cut(s) 3462
BseDI CCNNGG 5 cut(s) 94, 849, 1750, 3486, 3960
BseJI GATNNNNATC 2 cut(s) 951, 3234
BseMI GCAATG 1 cut(s) 3840
BseMII CTCAG 9 cut(s) 68, 363, 435, 1268, 1428, 1452, 1941, 2160, 2626
BseNI ACTGG 7 cut(s) 998, 1087, 1801, 2483, 3290, 3434, 4184
BseRI GAGGAG 3 cut(s) 245, 1775, 3455
BseYI CCCAGC 1 cut(s) 507
Bsh1236I CGCG 1 cut(s) 990
BshFI GGCC 8 cut(s) 59, 126, 1197, 1755, 2259, 3334, 3996, 4104
BshNI GGYRCC 1 cut(s) 4145
BshTI ACCGGT 1 cut(s) 465
BshVI ATCGAT 1 cut(s) 3462
BsiHKAI GWGCWC 2 cut(s) 2790, 3394
BsiHKCI CYCGRG 2 cut(s) 2087, 3668
BsiSI CCGG 6 cut(s) 466, 1485, 1769, 2234, 3960, 4143
BslFI GGGAC 3 cut(s) 2746, 3687, 3977
BsmFI GGGAC 3 cut(s) 2746, 3687, 3977
BsmI GAATGC 3 cut(s) 22, 320, 1694
BsnI GGCC 8 cut(s) 59, 126, 1197, 1755, 2259, 3334, 3996, 4104
Bso31I GGTCTC 5 cut(s) 1590, 1946, 2720, 3671, 3794
BsoBI CYCGRG 2 cut(s) 2087, 3668
Bsp1286I GDGCHC 2 cut(s) 2790, 3394
Bsp13I TCCGGA 1 cut(s) 1768
Bsp1407I TGTACA 1 cut(s) 514
Bsp68I TCGCGA 1 cut(s) 990
BspACI CCGC 8 cut(s) 21, 109, 3357, 3645, 4106, 4115, 4149, 4180
BspANI GGCC 8 cut(s) 59, 126, 1197, 1755, 2259, 3334, 3996, 4104
BspCNI CTCAG 9 cut(s) 67, 364, 436, 1269, 1429, 1453, 1942, 2161, 2625
BspDI ATCGAT 1 cut(s) 3462
BspEI TCCGGA 1 cut(s) 1768
BspFNI CGCG 1 cut(s) 990
BspHI TCATGA 1 cut(s) 1588
BspLI GGNNCC 5 cut(s) 366, 1225, 2166, 2908, 4147
BspMAI CTGCAG 3 cut(s) 28, 3904, 4128
BspMI ACCTGC 1 cut(s) 2152
BspPI GGATC 7 cut(s) 359, 372, 960, 1553, 2298, 2646, 3243
BspQI GCTCTTC 1 cut(s) 2367
BspT107I GGYRCC 1 cut(s) 4145
BspTI CTTAAG 2 cut(s) 500, 1807
BspTNI GGTCTC 5 cut(s) 1590, 1946, 2720, 3671, 3794
BsrDI GCAATG 1 cut(s) 3840
BsrFI RCCGGY 1 cut(s) 465
BsrGI TGTACA 1 cut(s) 514
BsrI ACTGG 7 cut(s) 998, 1087, 1801, 2483, 3290, 3434, 4184
BssAI RCCGGY 1 cut(s) 465
BssECI CCNNGG 5 cut(s) 94, 849, 1750, 3486, 3960
BssNI GRCGYC 2 cut(s) 3863, 4146
BssT1I CCWWGG 1 cut(s) 1750
Bst2UI CCWGG 6 cut(s) 851, 1563, 2100, 2946, 3224, 3336
Bst6I CTCTTC 1 cut(s) 2367
BstACI GRCGYC 2 cut(s) 3863, 4146
BstAFI CTTAAG 2 cut(s) 500, 1807
BstAPI GCANNNNNTGC 1 cut(s) 1510
BstAUI TGTACA 1 cut(s) 514
BstBAI YACGTR 1 cut(s) 3691
BstC8I GCNNGC 9 cut(s) 9, 222, 243, 405, 1372, 3195, 3755, 4106, 4203
BstENI CCTNNNNNAGG 1 cut(s) 1749
BstFNI CGCG 1 cut(s) 990
BstH2I RGCGCY 2 cut(s) 2201, 4149
BstHHI GCGC 3 cut(s) 381, 2200, 4148
BstMWI GCNNNNNNNGC 8 cut(s) 17, 1510, 3056, 3683, 3911, 4145, 4154, 4202
BstNI CCWGG 6 cut(s) 851, 1563, 2100, 2946, 3224, 3336
BstNSI RCATGY 3 cut(s) 1374, 2389, 4051
BstSCI CCNGG 7 cut(s) 849, 1561, 2098, 2944, 3222, 3334, 3959
BstSFI CTRYAG 4 cut(s) 24, 3599, 3900, 4124
BstUI CGCG 1 cut(s) 990
BstV2I GAAGAC 7 cut(s) 24, 39, 108, 1599, 1757, 2294, 2627
BstX2I RGATCY 8 cut(s) 364, 791, 1811, 1993, 2272, 2290, 2527, 3709
BstXI CCANNNNNNTGG 1 cut(s) 850
BstYI RGATCY 8 cut(s) 364, 791, 1811, 1993, 2272, 2290, 2527, 3709
Bsu15I ATCGAT 1 cut(s) 3462
BsuI GTATCC 4 cut(s) 684, 1812, 3038, 3268
BsuRI GGCC 8 cut(s) 59, 126, 1197, 1755, 2259, 3334, 3996, 4104
BsuTUI ATCGAT 1 cut(s) 3462
BtgZI GCGATG 1 cut(s) 4182
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 6 cut(s) 33, 1080, 2490, 2620, 3411, 3608
BtuMI TCGCGA 1 cut(s) 990
BveI ACCTGC 1 cut(s) 2152
Cac8I GCNNGC 9 cut(s) 9, 222, 243, 405, 1372, 3195, 3755, 4106, 4203
CciI TCATGA 1 cut(s) 1588
CfoI GCGC 3 cut(s) 381, 2200, 4148
Cfr10I RCCGGY 1 cut(s) 465
Cfr13I GGNCC 4 cut(s) 58, 3332, 4103, 4177
ClaI ATCGAT 1 cut(s) 3462
CseI GACGC 1 cut(s) 3852
CspAI ACCGGT 1 cut(s) 465
DinI GGCGCC 1 cut(s) 4147
DraI TTTAAA 2 cut(s) 1381, 3115
DraIII CACNNNGTG 3 cut(s) 361, 1606, 3694
DrdI GACNNNNNNGTC 1 cut(s) 2494
DseDI GACNNNNNNGTC 1 cut(s) 2494
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 2367
EarI CTCTTC 1 cut(s) 2367
Ecl136II GAGCTC 2 cut(s) 2788, 3392
Eco130I CCWWGG 1 cut(s) 1750
Eco147I AGGCCT 1 cut(s) 1755
Eco24I GRGCYC 2 cut(s) 2790, 3394
Eco31I GGTCTC 5 cut(s) 1590, 1946, 2720, 3671, 3794
Eco47I GGWCC 1 cut(s) 4177
Eco47III AGCGCT 1 cut(s) 2199
Eco53kI GAGCTC 2 cut(s) 2788, 3392
Eco57I CTGAAG 3 cut(s) 2537, 2900, 4173
Eco88I CYCGRG 2 cut(s) 2087, 3668
EcoICRI GAGCTC 2 cut(s) 2788, 3392
EcoNI CCTNNNNNAGG 1 cut(s) 1749
EcoRI GAATTC 2 cut(s) 156, 2022
EcoRII CCWGG 6 cut(s) 849, 1561, 2098, 2944, 3222, 3334
EcoT14I CCWWGG 1 cut(s) 1750
EcoT22I ATGCAT 3 cut(s) 586, 1506, 3991
EcoT38I GRGCYC 2 cut(s) 2790, 3394
EgeI GGCGCC 1 cut(s) 4147
EheI GGCGCC 1 cut(s) 4147
ErhI CCWWGG 1 cut(s) 1750
FalI AAGNNNNNCTT 6 cut(s) 819, 851, 2911, 2943, 4146, 4178
FaqI GGGAC 3 cut(s) 2746, 3687, 3977
FauI CCCGC 2 cut(s) 116, 4113
FauNDI CATATG 1 cut(s) 653
FbaI TGATCA 2 cut(s) 742, 1876
FblI GTMKAC 1 cut(s) 597
FriOI GRGCYC 2 cut(s) 2790, 3394
FspBI CTAG 8 cut(s) 815, 1004, 2597, 2913, 3071, 3699, 3797, 4013
GlaI GCGC 3 cut(s) 380, 2199, 4147
GsaI CCCAGC 1 cut(s) 511
GsuI CTGGAG 1 cut(s) 1002
HaeII RGCGCY 2 cut(s) 2201, 4149
HaeIII GGCC 8 cut(s) 59, 126, 1197, 1755, 2259, 3334, 3996, 4104
HapII CCGG 6 cut(s) 466, 1485, 1769, 2234, 3960, 4143
HgaI GACGC 1 cut(s) 3852
HhaI GCGC 3 cut(s) 381, 2200, 4148
Hin1I GRCGYC 2 cut(s) 3863, 4146
Hin6I GCGC 3 cut(s) 379, 2198, 4146
HinP1I GCGC 3 cut(s) 379, 2198, 4146
HincII GTYRAC 2 cut(s) 598, 1449
HindII GTYRAC 2 cut(s) 598, 1449
HindIII AAGCTT 4 cut(s) 239, 2663, 3751, 3945
HpaI GTTAAC 1 cut(s) 1449
HpaII CCGG 6 cut(s) 466, 1485, 1769, 2234, 3960, 4143
HphI GGTGA 7 cut(s) 252, 746, 1136, 1931, 3358, 3780, 3968
Hpy166II GTNNAC 8 cut(s) 598, 677, 1449, 2549, 2855, 3606, 3910, 4007
Hpy8I GTNNAC 8 cut(s) 598, 677, 1449, 2549, 2855, 3606, 3910, 4007
Hpy99I CGWCG 3 cut(s) 46, 125, 602
HpyCH4IV ACGT 8 cut(s) 360, 766, 1570, 1632, 3471, 3577, 3690, 3966
HpyF10VI GCNNNNNNNGC 8 cut(s) 17, 1510, 3056, 3683, 3911, 4145, 4154, 4202
HpySE526I ACGT 8 cut(s) 360, 766, 1570, 1632, 3471, 3577, 3690, 3966
Hsp92I GRCGYC 2 cut(s) 3863, 4146
HspAI GCGC 3 cut(s) 379, 2198, 4146
KasI GGCGCC 1 cut(s) 4145
Kpn2I TCCGGA 1 cut(s) 1768
Ksp22I TGATCA 2 cut(s) 742, 1876
KspAI GTTAAC 1 cut(s) 1449
LguI GCTCTTC 1 cut(s) 2367
LmnI GCTCC 6 cut(s) 4, 424, 890, 2561, 3389, 3762
LweI GCATC 9 cut(s) 593, 690, 1491, 1750, 2345, 2451, 2655, 3820, 4205
MaeI CTAG 8 cut(s) 815, 1004, 2597, 2913, 3071, 3699, 3797, 4013
MaeII ACGT 8 cut(s) 360, 766, 1570, 1632, 3471, 3577, 3690, 3966
MfeI CAATTG 2 cut(s) 225, 2241
MflI RGATCY 8 cut(s) 364, 791, 1811, 1993, 2272, 2290, 2527, 3709
MhlI GDGCHC 2 cut(s) 2790, 3394
Mly113I GGCGCC 1 cut(s) 4146
MlyI GAGTC 8 cut(s) 242, 1171, 1556, 1776, 2101, 2126, 2246, 3584
MmeI TCCRAC 7 cut(s) 1476, 1855, 1999, 2370, 3717, 3807, 3999
Mph1103I ATGCAT 3 cut(s) 586, 1506, 3991
MroI TCCGGA 1 cut(s) 1768
MroXI GAANNNNTTC 5 cut(s) 81, 834, 1356, 2828, 2885
MslI CAYNNNNRTG 6 cut(s) 283, 654, 759, 2253, 3455, 3984
MspA1I CMGCKG 3 cut(s) 23, 3905, 4151
MspCI CTTAAG 2 cut(s) 500, 1807
MspI CCGG 6 cut(s) 466, 1485, 1769, 2234, 3960, 4143
MspR9I CCNGG 7 cut(s) 851, 1563, 2100, 2946, 3224, 3336, 3961
MunI CAATTG 2 cut(s) 225, 2241
Mva1269I GAATGC 3 cut(s) 22, 320, 1694
MvaI CCWGG 6 cut(s) 851, 1563, 2100, 2946, 3224, 3336
MvnI CGCG 1 cut(s) 990
MwoI GCNNNNNNNGC 8 cut(s) 17, 1510, 3056, 3683, 3911, 4145, 4154, 4202
NarI GGCGCC 1 cut(s) 4146
NciI CCSGG 1 cut(s) 3961
NdeI CATATG 1 cut(s) 653
NlaIV GGNNCC 5 cut(s) 366, 1225, 2166, 2908, 4147
NmuCI GTSAC 4 cut(s) 752, 1078, 2485, 3472
NruI TCGCGA 1 cut(s) 990
NsiI ATGCAT 3 cut(s) 586, 1506, 3991
NspI RCATGY 3 cut(s) 1374, 2389, 4051
OliI CACNNNNGTG 2 cut(s) 654, 2253
PaeI GCATGC 1 cut(s) 1374
PaeR7I CTCGAG 2 cut(s) 2087, 3668
PagI TCATGA 1 cut(s) 1588
PceI AGGCCT 1 cut(s) 1755
PciI ACATGT 1 cut(s) 4047
PciSI GCTCTTC 1 cut(s) 2367
PcsI WCGNNNNNNNCGW 1 cut(s) 3468
PctI GAATGC 3 cut(s) 22, 320, 1694
PdmI GAANNNNTTC 5 cut(s) 81, 834, 1356, 2828, 2885
PfeI GAWTC 7 cut(s) 545, 620, 1684, 1772, 2608, 3230, 3626
PfoI TCCNGGA 1 cut(s) 2944
PinAI ACCGGT 1 cut(s) 465
PleI GAGTC 8 cut(s) 241, 1170, 1555, 1776, 2100, 2125, 2245, 3584
PluTI GGCGCC 1 cut(s) 4149
PpsI GAGTC 8 cut(s) 241, 1170, 1555, 1776, 2100, 2125, 2245, 3584
Ppu21I YACGTR 1 cut(s) 3691
PscI ACATGT 1 cut(s) 4047
PsiI TTATAA 1 cut(s) 3725
Psp124BI GAGCTC 2 cut(s) 2790, 3394
Psp6I CCWGG 6 cut(s) 849, 1561, 2098, 2944, 3222, 3334
PspFI CCCAGC 1 cut(s) 507
PspGI CCWGG 6 cut(s) 849, 1561, 2098, 2944, 3222, 3334
PspN4I GGNNCC 5 cut(s) 366, 1225, 2166, 2908, 4147
PspPI GGNCC 4 cut(s) 58, 3332, 4103, 4177
PstI CTGCAG 3 cut(s) 28, 3904, 4128
PsuI RGATCY 8 cut(s) 364, 791, 1811, 1993, 2272, 2290, 2527, 3709
PvuII CAGCTG 1 cut(s) 3905
RruI TCGCGA 1 cut(s) 990
RseI CAYNNNNRTG 6 cut(s) 283, 654, 759, 2253, 3455, 3984
SacI GAGCTC 2 cut(s) 2790, 3394
SalI GTCGAC 1 cut(s) 596
SapI GCTCTTC 1 cut(s) 2367
Sau96I GGNCC 4 cut(s) 58, 3332, 4103, 4177
SchI GAGTC 8 cut(s) 242, 1171, 1556, 1776, 2101, 2126, 2246, 3584
ScrFI CCNGG 7 cut(s) 851, 1563, 2100, 2946, 3224, 3336, 3961
SduI GDGCHC 2 cut(s) 2790, 3394
SfaNI GCATC 9 cut(s) 593, 690, 1491, 1750, 2345, 2451, 2655, 3820, 4205
SfcI CTRYAG 4 cut(s) 24, 3599, 3900, 4124
SfoI GGCGCC 1 cut(s) 4147
Sfr274I CTCGAG 2 cut(s) 2087, 3668
SinI GGWCC 1 cut(s) 4177
SlaI CTCGAG 2 cut(s) 2087, 3668
SmiMI CAYNNNNRTG 6 cut(s) 283, 654, 759, 2253, 3455, 3984
SmlI CTYRAG 9 cut(s) 185, 500, 923, 1777, 1807, 1982, 2087, 2666, 3668
SmoI CTYRAG 9 cut(s) 185, 500, 923, 1777, 1807, 1982, 2087, 2666, 3668
SpeI ACTAGT 1 cut(s) 2596
SphI GCATGC 1 cut(s) 1374
SseBI AGGCCT 1 cut(s) 1755
SsiI CCGC 8 cut(s) 21, 109, 3357, 3645, 4106, 4115, 4149, 4180
SspDI GGCGCC 1 cut(s) 4145
SspI AATATT 2 cut(s) 1356, 1826
SspMI CTAG 8 cut(s) 815, 1004, 2597, 2913, 3071, 3699, 3797, 4013
SstI GAGCTC 2 cut(s) 2790, 3394
StuI AGGCCT 1 cut(s) 1755
StyD4I CCNGG 7 cut(s) 849, 1561, 2098, 2944, 3222, 3334, 3959
StyI CCWWGG 1 cut(s) 1750
TaiI ACGT 8 cut(s) 363, 769, 1573, 1635, 3474, 3580, 3693, 3969
TatI WGTACW 4 cut(s) 514, 1028, 1180, 3067
TauI GCSGC 3 cut(s) 23, 4118, 4151
TfiI GAWTC 7 cut(s) 545, 620, 1684, 1772, 2608, 3230, 3626
TscAI CASTG 6 cut(s) 33, 1087, 2490, 2620, 3418, 3608
TseFI GTSAC 4 cut(s) 752, 1078, 2485, 3472
Tsp45I GTSAC 4 cut(s) 752, 1078, 2485, 3472
TspGWI ACGGA 6 cut(s) 357, 437, 3296, 3719, 3753, 3896
TspRI CASTG 6 cut(s) 33, 1087, 2490, 2620, 3418, 3608
Vha464I CTTAAG 2 cut(s) 500, 1807
VpaK11BI GGWCC 1 cut(s) 4177
XagI CCTNNNNNAGG 1 cut(s) 1749
XapI RAATTY 7 cut(s) 156, 1702, 2022, 2769, 2885, 2955, 4221
XbaI TCTAGA 1 cut(s) 3796
XceI RCATGY 3 cut(s) 1374, 2389, 4051
XcmI CCANNNNNNNNNTGG 2 cut(s) 850, 2490
XhoI CTCGAG 2 cut(s) 2087, 3668
XmiI GTMKAC 1 cut(s) 597
XmnI GAANNNNTTC 5 cut(s) 81, 834, 1356, 2828, 2885
XspI CTAG 8 cut(s) 815, 1004, 2597, 2913, 3071, 3699, 3797, 4013
Zsp2I ATGCAT 3 cut(s) 586, 1506, 3991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.