Rw1G004610

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Reverse (-)
8844204 .. 8864429
20226 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004610.1

Sequence Viewer

Length: 3144 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGCTTTTCTAAGTTTCAGGGGGATAGATACGCGCAAGAATTTTACCTCTCTTCTGTATTCTACACTAAATCATAATGGAATTCTCACCTTTCGGGATGACCCAGAGCTTGAGAAAGGAAAATCCATTGGGCCTGAACTTTTAGCTGCAATTGAGGAGTCTAGATTTGCTATTGTCATTCTCTCACGCACCTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGACAAGGGCCAACAAGTCCTCCCCGTCTTCTACAGGGTGGATCCTTCTGATGTGCGGCACCAAAGGGGAAGATTTGAGCTCAAATGGGAACACCGAGTAGATGTGGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGAATGAGGACAGACTAAATGCCTGGACAGCTGCTTTGACTGAGGTCGCCAATCTTTCTGGCTGGCATTCCGATTTTTATCCACATGATTCAAAAGTAATTGAGGAGATTGCTAATCATATACTAAAGAAGTCCCTGTATATATCTTCAAGTGTTGACCAGGGCTTCATAGGAATGGATTCCCGCATTCATGATTTATTAAGCAATTACATATATCCGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATCAAATTTGTCAAAATTATGACCGAACCTGCTTTCTTTCCAATGTTAGAGAACTGTCTAAAAACAATGGCCTAGTGTCTCTACAAGAAAAACTTCTTTCCAAAATCCTGATGGCAAAAATTGAAAATATAGAGGATGAATGCACAGGAACTGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGATGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCGGGGAGTAGAATTATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAAGCTAACGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATTACCCCCACCAGAAGATTATTTGGATCTGTGCTACCATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTCTTTGGTAGAAGCACTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCACATAAACGCATTATTGACGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTGAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAAAGAACTGTGGATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCCCAAAGAGCCAGGCAAACAAAGTAGATTATGGTATGATGAAGACGTCAACAATGTGCTGAAGAAAAATAAGGGAACAGATTCAATTCAAGGCATGGTAATGGAGTTGCCTAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTATCTCAACTTAGTCTTTTCCATATTCGTAATGTGGACCTTCCCAACGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTGAGATCTCTCCCACAAAATTTTGAAGCAGATGAACTTATGGAACTTAACTTGTGCCACAGCAACATTGAACAGCTTTGGACGGGAACTAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAGCATTGAGGAGACCCCAGACCTTGCAGGCGTTCCGAATCTTGAAACTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTGAAAGACTGCAAAAGTCTTGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGGTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAATGGCTGAGTGGCCTTGTGTCATTGAATCTAACCAACTGCAAAAATCTTGTTTGTCTTCCAAGCACCATCAATAAGTTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAATGTGGGGGTGATGGAGTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCATTTAAGCGTGATCACAGAAAATATGTGAGAGGTTCAATCTTTCATCGATGCAAAGTAGTTTGGCGATCTTTAAAAAAGGTCTTGCCTTCTGGATTGGTGCAACAAGTGAATACAGAGCCAAAGAGTTTCCACTTGCCTATATCACAAAAAGTGAATACAGAGCCAATGAGTTTCTGCTTGCCTATATCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAATTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTAAGTGGTTCAGCCATCAGAGTGATGGGTGTTCTGTAAGTGTATCTCTACCTGCACATTGGAATAACAGTCGGGTTTTGGGATTTGCTCTGTGTGCTGTTTTTGTACTCCATGAGCATCTGGTGGATAAGCTTTATGTAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTGTAAGAATTTGTGGACTTATCAATCTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

1047

Amino Acids

119.0

Weight (kDa)

6.46

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 191 1.2e-50 TIR domain
TIR_2 PF13676 15 - 110 1.2e-11 TIR domain
NB-ARC PF00931 217 - 371 2.7e-18 NB-ARC domain
NACHT PF05729 221 - 318 3.6e-07 NACHT domain
WHD_ROQ1 PF23282 438 - 509 2e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 698 - 783 6.3e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 709 - 777 1.6e-06 Leucine-rich repeat region
C-JID PF20160 971 - 1021 1.2e-14 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1575
Acc36I ACCTGC 2 cut(s) 755, 2989
AccB1I GGYRCC 1 cut(s) 342
AccI GTMKAC 1 cut(s) 2704
AccII CGCG 1 cut(s) 70
AciI CCGC 5 cut(s) 340, 604, 638, 679, 886
AclI AACGTT 1 cut(s) 2167
AclWI GGATC 7 cut(s) 320, 333, 661, 674, 936, 1146, 1380
AcuI CTGAAG 2 cut(s) 1607, 2876
AcyI GRCGYC 2 cut(s) 5, 1572
AfaI GTAC 2 cut(s) 2731, 3036
AfiI CCNNNNNNNGG 4 cut(s) 644, 1418, 2150, 2615
AjiI CACGTC 1 cut(s) 1285
AjnI CCWGG 4 cut(s) 443, 579, 1537, 2914
AjuI GAANNNNNNNTTGG 2 cut(s) 2636, 2668
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 6 cut(s) 801, 1530, 1750, 1922, 2186, 2662
AlwI GGATC 7 cut(s) 320, 333, 661, 674, 936, 1146, 1380
AlwNI CAGNNNCTG 1 cut(s) 869
Ama87I CYCGRG 2 cut(s) 705, 2144
AoxI GGCC 5 cut(s) 167, 292, 787, 1729, 2236
ApeKI GCWGC 3 cut(s) 182, 452, 640
ArsI GACNNNNNNTTYG 2 cut(s) 439, 471
AseI ATTAAT 1 cut(s) 2108
Asp700I GAANNNNTTC 7 cut(s) 598, 810, 1080, 1512, 1606, 2861, 2898
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 4 cut(s) 167, 292, 977, 1717
AsuC2I CCSGG 1 cut(s) 981
AsuHPI GGTGA 2 cut(s) 115, 2383
AvaI CYCGRG 2 cut(s) 705, 2144
AvaII GGWCC 2 cut(s) 977, 1717
BamHI GGATCC 2 cut(s) 325, 666
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 1187
BbsI GAAGAC 4 cut(s) 304, 1575, 2273, 2864
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 169, 439, 652
BccI CCATC 9 cut(s) 823, 1298, 2010, 2300, 2368, 2701, 2717, 2948, 2952
BceAI ACGGC 1 cut(s) 1744
BciT130I CCWGG 4 cut(s) 445, 581, 1539, 2916
BciVI GTATCC 1 cut(s) 1788
BclI TGATCA 2 cut(s) 718, 2437
BcnI CCSGG 1 cut(s) 981
BcoDI GTCTC 6 cut(s) 801, 1530, 1750, 1922, 2186, 2662
BfaI CTAG 4 cut(s) 198, 791, 1391, 2708
BfmI CTRYAG 1 cut(s) 316
BfuAI ACCTGC 2 cut(s) 755, 2989
BfuI GTATCC 1 cut(s) 1788
BglII AGATCT 3 cut(s) 1442, 1787, 1969
BisI GCNGC 4 cut(s) 183, 341, 453, 641
BlsI GCNGC 4 cut(s) 184, 342, 454, 642
Bme1390I CCNGG 5 cut(s) 445, 581, 981, 1539, 2916
Bme18I GGWCC 2 cut(s) 977, 1717
BmeT110I CYCGRG 2 cut(s) 705, 2144
BmgBI CACGTC 1 cut(s) 1285
BmgT120I GGNCC 4 cut(s) 167, 292, 977, 1717
BmiI GGNNCC 3 cut(s) 327, 344, 668
BmrFI CCNGG 5 cut(s) 445, 581, 981, 1539, 2916
BmrI ACTGGG 1 cut(s) 1782
BmuI ACTGGG 1 cut(s) 1782
BoxI GACNNNNGTC 1 cut(s) 464
BpiI GAAGAC 4 cut(s) 304, 1575, 2273, 2864
Bpu10I CCTNAGC 1 cut(s) 1167
BpuEI CTTGAG 5 cut(s) 167, 1774, 1804, 2084, 2660
BpuMI CCSGG 1 cut(s) 981
Bsa29I ATCGAT 1 cut(s) 2473
BsaBI GATNNNNATC 3 cut(s) 498, 927, 1371
BsaHI GRCGYC 2 cut(s) 5, 1572
BsaI GGTCTC 1 cut(s) 1922
BsaJI CCNNGG 4 cut(s) 580, 670, 980, 2011
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 4 cut(s) 644, 1418, 2150, 2615
Bse1I ACTGG 3 cut(s) 974, 1314, 1777
Bse8I GATNNNNATC 3 cut(s) 498, 927, 1371
BseBI CCWGG 4 cut(s) 445, 581, 1539, 2916
BseCI ATCGAT 1 cut(s) 2473
BseDI CCNNGG 4 cut(s) 580, 670, 980, 2011
BseGI GGATG 8 cut(s) 139, 259, 859, 1318, 1482, 1985, 2339, 2834
BseJI GATNNNNATC 3 cut(s) 498, 927, 1371
BseLI CCNNNNNNNGG 4 cut(s) 644, 1418, 2150, 2615
BseMII CTCAG 3 cut(s) 453, 1181, 2222
BseNI ACTGG 3 cut(s) 974, 1314, 1777
BseRI GAGGAG 4 cut(s) 206, 539, 1751, 1940
BseXI GCAGC 3 cut(s) 169, 439, 652
BseYI CCCAGC 1 cut(s) 1503
BsgI GTGCAG 1 cut(s) 2967
Bsh1236I CGCG 1 cut(s) 70
BshFI GGCC 5 cut(s) 169, 294, 789, 1731, 2238
BshNI GGYRCC 1 cut(s) 342
BshVI ATCGAT 1 cut(s) 2473
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 2 cut(s) 705, 2144
BsiSI CCGG 1 cut(s) 980
BslFI GGGAC 1 cut(s) 538
BslI CCNNNNNNNGG 4 cut(s) 644, 1418, 2150, 2615
BsmAI GTCTC 6 cut(s) 801, 1530, 1750, 1922, 2186, 2662
BsmFI GGGAC 1 cut(s) 538
BsmI GAATGC 3 cut(s) 487, 606, 863
BsnI GGCC 5 cut(s) 169, 294, 789, 1731, 2238
Bso31I GGTCTC 1 cut(s) 1922
BsoBI CYCGRG 2 cut(s) 705, 2144
Bsp1286I GDGCHC 1 cut(s) 366
Bsp19I CCATGG 1 cut(s) 670
BspACI CCGC 5 cut(s) 340, 604, 638, 679, 886
BspANI GGCC 5 cut(s) 169, 294, 789, 1731, 2238
BspCNI CTCAG 3 cut(s) 454, 1180, 2223
BspDI ATCGAT 1 cut(s) 2473
BspFNI CGCG 1 cut(s) 70
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 3 cut(s) 327, 344, 668
BspMI ACCTGC 2 cut(s) 755, 2989
BspPI GGATC 7 cut(s) 320, 333, 661, 674, 936, 1146, 1380
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 1 cut(s) 1922
BsrI ACTGG 3 cut(s) 974, 1314, 1777
BssECI CCNNGG 4 cut(s) 580, 670, 980, 2011
BssNAI GTATAC 1 cut(s) 2705
BssNI GRCGYC 2 cut(s) 5, 1572
BssSI CACGAG 1 cut(s) 1187
BssT1I CCWWGG 2 cut(s) 670, 2011
Bst1107I GTATAC 1 cut(s) 2705
Bst2BI CACGAG 1 cut(s) 1187
Bst2UI CCWGG 4 cut(s) 445, 581, 1539, 2916
Bst4CI ACNGT 5 cut(s) 774, 1070, 1473, 1524, 2999
Bst6I CTCTTC 2 cut(s) 19, 93
BstACI GRCGYC 2 cut(s) 5, 1572
BstAPI GCANNNNNTGC 1 cut(s) 1486
BstC8I GCNNGC 6 cut(s) 485, 677, 1348, 1403, 1945, 2606
BstDEI CTNAG 8 cut(s) 47, 462, 1167, 1691, 1872, 2231, 2634, 2931
BstDSI CCRYGG 1 cut(s) 670
BstENI CCTNNNNNAGG 1 cut(s) 2613
BstF5I GGATG 8 cut(s) 139, 259, 859, 1318, 1482, 1985, 2339, 2834
BstFNI CGCG 1 cut(s) 70
BstHHI GCGC 1 cut(s) 72
BstMAI GTCTC 6 cut(s) 801, 1530, 1750, 1922, 2186, 2662
BstMWI GCNNNNNNNGC 5 cut(s) 449, 681, 1486, 2635, 3023
BstNI CCWGG 4 cut(s) 445, 581, 1539, 2916
BstNSI RCATGY 1 cut(s) 679
BstPAI GACNNNNGTC 1 cut(s) 464
BstSCI CCNGG 5 cut(s) 443, 579, 979, 1537, 2914
BstSFI CTRYAG 1 cut(s) 316
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 3 cut(s) 169, 439, 652
BstV2I GAAGAC 4 cut(s) 304, 1575, 2273, 2864
BstX2I RGATCY 6 cut(s) 325, 666, 1138, 1442, 1787, 1969
BstYI RGATCY 6 cut(s) 325, 666, 1138, 1442, 1787, 1969
BstZ17I GTATAC 1 cut(s) 2705
Bsu15I ATCGAT 1 cut(s) 2473
BsuI GTATCC 1 cut(s) 1788
BsuRI GGCC 5 cut(s) 169, 294, 789, 1731, 2238
BsuTUI ATCGAT 1 cut(s) 2473
BtgI CCRYGG 1 cut(s) 670
BtrI CACGTC 1 cut(s) 1285
BtsCI GGATG 8 cut(s) 139, 259, 859, 1318, 1482, 1985, 2339, 2834
BtsIMutI CAGTG 2 cut(s) 1221, 2735
BveI ACCTGC 2 cut(s) 755, 2989
Cac8I GCNNGC 6 cut(s) 485, 677, 1348, 1403, 1945, 2606
CaiI CAGNNNCTG 1 cut(s) 869
CciI TCATGA 1 cut(s) 610
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 4 cut(s) 167, 292, 977, 1717
ClaI ATCGAT 1 cut(s) 2473
Csp6I GTAC 2 cut(s) 2730, 3035
CviQI GTAC 2 cut(s) 2730, 3035
DdeI CTNAG 8 cut(s) 47, 462, 1167, 1691, 1872, 2231, 2634, 2931
DraI TTTAAA 1 cut(s) 2499
Eam1104I CTCTTC 2 cut(s) 19, 93
EarI CTCTTC 2 cut(s) 19, 93
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 2 cut(s) 670, 2011
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 1922
Eco47I GGWCC 2 cut(s) 977, 1717
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 2 cut(s) 1607, 2876
Eco88I CYCGRG 2 cut(s) 705, 2144
EcoICRI GAGCTC 1 cut(s) 364
EcoNI CCTNNNNNAGG 1 cut(s) 2613
EcoRI GAATTC 2 cut(s) 117, 3074
EcoRII CCWGG 4 cut(s) 443, 579, 1537, 2914
EcoT14I CCWWGG 2 cut(s) 670, 2011
EcoT22I ATGCAT 3 cut(s) 281, 1482, 2758
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 2 cut(s) 670, 2011
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FaqI GGGAC 1 cut(s) 538
FauI CCCGC 2 cut(s) 611, 672
FbaI TGATCA 2 cut(s) 718, 2437
FblI GTMKAC 1 cut(s) 2704
Fnu4HI GCNGC 4 cut(s) 183, 341, 453, 641
FokI GGATG 8 cut(s) 146, 266, 866, 1325, 1489, 1992, 2346, 2841
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 4 cut(s) 183, 341, 453, 641
FspBI CTAG 4 cut(s) 198, 791, 1391, 2708
GlaI GCGC 1 cut(s) 71
GluI GCNGC 4 cut(s) 183, 341, 453, 641
GsaI CCCAGC 1 cut(s) 1507
HaeIII GGCC 5 cut(s) 169, 294, 789, 1731, 2238
HapII CCGG 1 cut(s) 980
HhaI GCGC 1 cut(s) 72
Hin1I GRCGYC 2 cut(s) 5, 1572
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 4 cut(s) 9, 577, 936, 1576
HindII GTYRAC 4 cut(s) 9, 577, 936, 1576
HindIII AAGCTT 3 cut(s) 2027, 2636, 3059
HpaII CCGG 1 cut(s) 980
HphI GGTGA 2 cut(s) 115, 2383
Hpy166II GTNNAC 8 cut(s) 9, 577, 936, 1520, 1576, 1717, 2705, 3125
Hpy188I TCNGA 7 cut(s) 334, 493, 1293, 1953, 2046, 2686, 2949
Hpy8I GTNNAC 8 cut(s) 9, 577, 936, 1520, 1576, 1717, 2705, 3125
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 7 cut(s) 339, 1093, 1357, 1730, 1970, 2523, 2699
HpyCH4III ACNGT 5 cut(s) 774, 1070, 1473, 1524, 2999
HpyCH4IV ACGT 3 cut(s) 1284, 1572, 2167
HpyF10VI GCNNNNNNNGC 5 cut(s) 449, 681, 1486, 2635, 3023
HpyF3I CTNAG 8 cut(s) 47, 462, 1167, 1691, 1872, 2231, 2634, 2931
HpySE526I ACGT 3 cut(s) 1284, 1572, 2167
Hsp92I GRCGYC 2 cut(s) 5, 1572
HspAI GCGC 1 cut(s) 70
Ksp22I TGATCA 2 cut(s) 718, 2437
Lsp1109I GCAGC 3 cut(s) 169, 439, 652
MaeI CTAG 4 cut(s) 198, 791, 1391, 2708
MaeII ACGT 3 cut(s) 1284, 1572, 2167
MaeIII GTNAC 1 cut(s) 1378
MfeI CAATTG 3 cut(s) 186, 2220, 2800
MflI RGATCY 6 cut(s) 325, 666, 1138, 1442, 1787, 1969
MhlI GDGCHC 1 cut(s) 366
MlyI GAGTC 3 cut(s) 203, 1752, 2077
MmeI TCCRAC 1 cut(s) 1485
Mph1103I ATGCAT 3 cut(s) 281, 1482, 2758
MroXI GAANNNNTTC 7 cut(s) 598, 810, 1080, 1512, 1606, 2861, 2898
MslI CAYNNNNRTG 3 cut(s) 593, 1625, 2949
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 1 cut(s) 980
MspR9I CCNGG 5 cut(s) 445, 581, 981, 1539, 2916
MunI CAATTG 3 cut(s) 186, 2220, 2800
Mva1269I GAATGC 3 cut(s) 487, 606, 863
MvaI CCWGG 4 cut(s) 445, 581, 1539, 2916
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 5 cut(s) 449, 681, 1486, 2635, 3023
NciI CCSGG 1 cut(s) 981
NcoI CCATGG 1 cut(s) 670
NlaIV GGNNCC 3 cut(s) 327, 344, 668
NmeAIII GCCGAG 1 cut(s) 2325
NmuCI GTSAC 1 cut(s) 1378
NsiI ATGCAT 3 cut(s) 281, 1482, 2758
NspI RCATGY 1 cut(s) 679
PaeI GCATGC 1 cut(s) 679
PaeR7I CTCGAG 1 cut(s) 705
PagI TCATGA 1 cut(s) 610
PctI GAATGC 3 cut(s) 487, 606, 863
PdmI GAANNNNTTC 7 cut(s) 598, 810, 1080, 1512, 1606, 2861, 2898
PfoI TCCNGGA 1 cut(s) 2914
PkrI GCNGC 4 cut(s) 184, 342, 454, 642
PleI GAGTC 3 cut(s) 202, 1752, 2076
PpsI GAGTC 3 cut(s) 202, 1752, 2076
PshAI GACNNNNGTC 1 cut(s) 464
PshBI ATTAAT 1 cut(s) 2108
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 1 cut(s) 2167
Psp6I CCWGG 4 cut(s) 443, 579, 1537, 2914
PspFI CCCAGC 1 cut(s) 1503
PspGI CCWGG 4 cut(s) 443, 579, 1537, 2914
PspN4I GGNNCC 3 cut(s) 327, 344, 668
PspPI GGNCC 4 cut(s) 167, 292, 977, 1717
PspXI VCTCGAGB 1 cut(s) 705
PstNI CAGNNNCTG 1 cut(s) 869
PsuI RGATCY 6 cut(s) 325, 666, 1138, 1442, 1787, 1969
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 2 cut(s) 2731, 3036
RsaNI GTAC 2 cut(s) 2730, 3035
RseI CAYNNNNRTG 3 cut(s) 593, 1625, 2949
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 4 cut(s) 183, 341, 453, 641
Sau96I GGNCC 4 cut(s) 167, 292, 977, 1717
SchI GAGTC 3 cut(s) 203, 1752, 2077
ScrFI CCNGG 5 cut(s) 445, 581, 981, 1539, 2916
SduI GDGCHC 1 cut(s) 366
SfcI CTRYAG 1 cut(s) 316
Sfr274I CTCGAG 1 cut(s) 705
SinI GGWCC 2 cut(s) 977, 1717
SlaI CTCGAG 1 cut(s) 705
SmiMI CAYNNNNRTG 3 cut(s) 593, 1625, 2949
SmlI CTYRAG 6 cut(s) 146, 705, 1753, 1783, 2063, 2639
SmoI CTYRAG 6 cut(s) 146, 705, 1753, 1783, 2063, 2639
SphI GCATGC 1 cut(s) 679
SsiI CCGC 5 cut(s) 340, 604, 638, 679, 886
SspI AATATT 2 cut(s) 1332, 2926
SspMI CTAG 4 cut(s) 198, 791, 1391, 2708
SstI GAGCTC 1 cut(s) 366
StyD4I CCNGG 5 cut(s) 443, 579, 979, 1537, 2914
StyI CCWWGG 2 cut(s) 670, 2011
TaaI ACNGT 5 cut(s) 774, 1070, 1473, 1524, 2999
TaiI ACGT 3 cut(s) 1287, 1575, 2170
TaqI TCGA 4 cut(s) 706, 1764, 2473, 3138
TaqII GACCGA 1 cut(s) 756
TatI WGTACW 2 cut(s) 2729, 3034
TauI GCSGC 1 cut(s) 343
TscAI CASTG 2 cut(s) 1228, 2742
TseFI GTSAC 1 cut(s) 1378
TseI GCWGC 3 cut(s) 182, 452, 640
Tsp45I GTSAC 1 cut(s) 1378
TspGWI ACGGA 1 cut(s) 1167
TspRI CASTG 2 cut(s) 1228, 2742
VpaK11BI GGWCC 2 cut(s) 977, 1717
VspI ATTAAT 1 cut(s) 2108
XagI CCTNNNNNAGG 1 cut(s) 2613
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 679
XcmI CCANNNNNNNNNTGG 3 cut(s) 826, 2009, 2951
XhoI CTCGAG 1 cut(s) 705
XmiI GTMKAC 1 cut(s) 2704
XmnI GAANNNNTTC 7 cut(s) 598, 810, 1080, 1512, 1606, 2861, 2898
XspI CTAG 4 cut(s) 198, 791, 1391, 2708
ZraI GACGTC 1 cut(s) 1573
Zsp2I ATGCAT 3 cut(s) 281, 1482, 2758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.