Rroxscaffold_4G00326620

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Reverse (-)
58771106 .. 58778128
7023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326620.1

Sequence Viewer

Length: 1095 bp
ATGGCTTCCTTCGTTTTTTTACAGTATCGGCTTGGATACGGCAGTGAAGTGGGTAATCTAAATTGTGTGGAGAGAAAGACGCCAGCAAGGGCGTACTCGAGACGCACCCACAGGTATAACGATTATGAATGTGAGGCCCCTTTTAGGTGGAAAGTGGAATCAAGTCCGGGCATTGACTCTGATTCAATTCAGATGGAGATCGATGGCGTCAAGCTCTTCTCGTCGTCAATGGAAATACGATGGGGAAGATACCCGCGCATATTTTACCGGTCTTCTGTATCTGCACTAAATCAGAAAGGAATTCTCACCTTTATGGATGACCCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAGCTGCAATTGAGGACTCTAGATCTGCGATTGTCATTCTCTCACCCACCTATGCTGATTCATCATGGTGCTTGAATGAACTGGGGATGGTTTCAGCTCAAAAGGAAACCCAAGTAGATGTGGAAGTAAGGGAACATGAAGAAGTTTATGGCAAGAATGAGGACAGACTATATGCCTGGAGAGCTGCTTTGACTGAGTTGCTAAAGGCACATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACTCTAATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATTTCCCCCACCAGAAGATTATTTGCACCTGTGCCACCATATTTTAGGGTATGCTCAGGGGCTTCCATTGGCTCTCGTGGTTTTAGGTTCTTTTCTCTTTGGTAGAAGCACTGATGAATGGACAAGTGCAACGGATAGGCTAAAGAACACACCACATAAACGCATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAGGACAGAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAGACCCTGTCATTGGTTTAAGTGTTCTTGCGGATAGATCTCTCATAACTATCTCCAACAACGAACTGTGGATGCATGATTTGCTACAAGAAATGGGCAGATTTACTTGGAACAGATTCAATCCAAGGCATGGTAACGGAGTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

364

Amino Acids

41.76

Weight (kDa)

5.87

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 91 - 152 8.1e-17 TIR domain
TIR_2 PF13676 94 - 155 1.6e-08 TIR domain
WHD_ROQ1 PF23282 285 - 349 1.2e-19 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1076
AccII CGCG 1 cut(s) 256
AciI CCGC 2 cut(s) 254, 977
AclWI GGATC 1 cut(s) 921
AcsI RAATTY 2 cut(s) 300, 653
AcyI GRCGYC 2 cut(s) 80, 207
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 3 cut(s) 144, 959, 1076
AgeI ACCGGT 1 cut(s) 267
AgsI TTSAA 5 cut(s) 186, 436, 626, 640, 1066
AjnI CCWGG 1 cut(s) 536
AluBI AGCT 6 cut(s) 214, 328, 365, 458, 545, 946
AluI AGCT 6 cut(s) 214, 328, 365, 458, 545, 946
Alw26I GTCTC 1 cut(s) 94
AlwI GGATC 1 cut(s) 921
Ama87I CYCGRG 1 cut(s) 97
AoxI GGCC 2 cut(s) 135, 350
ApeKI GCWGC 2 cut(s) 365, 545
ApoI RAATTY 2 cut(s) 300, 653
AsiGI ACCGGT 1 cut(s) 267
Asp700I GAANNNNTTC 2 cut(s) 621, 1061
AspLEI GCGC 1 cut(s) 258
AspS9I GGNCC 1 cut(s) 136
AsuC2I CCSGG 1 cut(s) 168
AsuHPI GGTGA 2 cut(s) 298, 396
AvaI CYCGRG 1 cut(s) 97
BaeI ACNNNNGTAYC 2 cut(s) 28, 61
BauI CACGAG 1 cut(s) 728
BbsI GAAGAC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 352, 532
BccI CCATC 5 cut(s) 187, 197, 234, 442, 839
BceAI ACGGC 1 cut(s) 55
BciT130I CCWGG 1 cut(s) 538
BciVI GTATCC 1 cut(s) 29
BcnI CCSGG 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 94
BfaI CTAG 3 cut(s) 381, 932, 947
BfuI GTATCC 1 cut(s) 29
BglII AGATCT 2 cut(s) 383, 983
BisI GCNGC 2 cut(s) 366, 546
BlsI GCNGC 2 cut(s) 367, 547
Bme1390I CCNGG 2 cut(s) 168, 538
BmeT110I CYCGRG 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 2 cut(s) 168, 538
BmrI ACTGGG 1 cut(s) 452
BmsI GCATC 2 cut(s) 571, 1008
BmuI ACTGGG 1 cut(s) 452
BpiI GAAGAC 1 cut(s) 264
BpmI CTGGAG 1 cut(s) 559
Bpu10I CCTNAGC 1 cut(s) 708
BpuEI CTTGAG 1 cut(s) 350
BpuMI CCSGG 1 cut(s) 168
Bsa29I ATCGAT 1 cut(s) 201
BsaBI GATNNNNATC 2 cut(s) 197, 912
BsaHI GRCGYC 2 cut(s) 80, 207
BsaJI CCNNGG 1 cut(s) 1070
BsaWI WCCGGW 1 cut(s) 267
Bsc4I CCNNNNNNNGG 3 cut(s) 144, 959, 1076
Bse118I RCCGGY 1 cut(s) 267
Bse1I ACTGG 2 cut(s) 447, 855
Bse8I GATNNNNATC 2 cut(s) 197, 912
BseBI CCWGG 1 cut(s) 538
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 1 cut(s) 1070
BseGI GGATG 4 cut(s) 322, 453, 859, 1023
BseJI GATNNNNATC 2 cut(s) 197, 912
BseLI CCNNNNNNNGG 3 cut(s) 144, 959, 1076
BseMII CTCAG 2 cut(s) 546, 722
BseNI ACTGG 2 cut(s) 447, 855
BseXI GCAGC 2 cut(s) 352, 532
BsgI GTGCAG 1 cut(s) 267
Bsh1236I CGCG 1 cut(s) 256
BshFI GGCC 2 cut(s) 137, 352
BshTI ACCGGT 1 cut(s) 267
BshVI ATCGAT 1 cut(s) 201
BsiHKCI CYCGRG 1 cut(s) 97
BsiSI CCGG 2 cut(s) 167, 268
BslI CCNNNNNNNGG 3 cut(s) 144, 959, 1076
BsmAI GTCTC 1 cut(s) 94
BsmBI CGTCTC 1 cut(s) 94
BsnI GGCC 2 cut(s) 137, 352
BsoBI CYCGRG 1 cut(s) 97
Bsp143I GATC 4 cut(s) 198, 383, 913, 983
BspACI CCGC 2 cut(s) 254, 977
BspANI GGCC 2 cut(s) 137, 352
BspCNI CTCAG 2 cut(s) 547, 721
BspDI ATCGAT 1 cut(s) 201
BspFNI CGCG 1 cut(s) 256
BspLI GGNNCC 1 cut(s) 138
BspPI GGATC 1 cut(s) 921
BspQI GCTCTTC 1 cut(s) 221
BsrFI RCCGGY 1 cut(s) 267
BsrI ACTGG 2 cut(s) 447, 855
BssAI RCCGGY 1 cut(s) 267
BssECI CCNNGG 1 cut(s) 1070
BssMI GATC 4 cut(s) 198, 383, 913, 983
BssNI GRCGYC 2 cut(s) 80, 207
BssSI CACGAG 1 cut(s) 728
BssT1I CCWWGG 1 cut(s) 1070
Bst2BI CACGAG 1 cut(s) 728
Bst2UI CCWGG 1 cut(s) 538
Bst4CI ACNGT 2 cut(s) 24, 1014
Bst6I CTCTTC 1 cut(s) 221
BstACI GRCGYC 2 cut(s) 80, 207
BstAPI GCANNNNNTGC 1 cut(s) 1027
BstC8I GCNNGC 3 cut(s) 84, 889, 944
BstDEI CTNAG 2 cut(s) 555, 708
BstF5I GGATG 4 cut(s) 322, 453, 859, 1023
BstFNI CGCG 1 cut(s) 256
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 4 cut(s) 201, 386, 916, 986
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 4 cut(s) 198, 383, 913, 983
BstMWI GCNNNNNNNGC 2 cut(s) 542, 1027
BstNI CCWGG 1 cut(s) 538
BstSCI CCNGG 2 cut(s) 166, 536
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 2 cut(s) 352, 532
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 2 cut(s) 383, 983
BstYI RGATCY 2 cut(s) 383, 983
Bsu15I ATCGAT 1 cut(s) 201
BsuI GTATCC 1 cut(s) 29
BsuRI GGCC 2 cut(s) 137, 352
BsuTUI ATCGAT 1 cut(s) 201
BtsCI GGATG 4 cut(s) 322, 453, 859, 1023
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 49, 762
Cac8I GCNNGC 3 cut(s) 84, 889, 944
CfoI GCGC 1 cut(s) 258
Cfr10I RCCGGY 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 136
ClaI ATCGAT 1 cut(s) 201
CseI GACGC 3 cut(s) 88, 111, 196
Csp6I GTAC 1 cut(s) 94
CspAI ACCGGT 1 cut(s) 267
CviAII CATG 5 cut(s) 426, 497, 572, 1022, 1076
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 2 cut(s) 555, 708
DpnI GATC 4 cut(s) 200, 385, 915, 985
DpnII GATC 4 cut(s) 198, 383, 913, 983
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 1070
Eco147I AGGCCT 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 300
EcoRII CCWGG 1 cut(s) 536
EcoT14I CCWWGG 1 cut(s) 1070
EcoT22I ATGCAT 1 cut(s) 1023
ErhI CCWWGG 1 cut(s) 1070
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 5 cut(s) 429, 500, 575, 1025, 1079
FatI CATG 5 cut(s) 425, 496, 571, 1021, 1075
FauI CCCGC 1 cut(s) 261
Fnu4HI GCNGC 2 cut(s) 366, 546
FokI GGATG 4 cut(s) 329, 460, 866, 1030
Fsp4HI GCNGC 2 cut(s) 366, 546
FspBI CTAG 3 cut(s) 381, 932, 947
GlaI GCGC 1 cut(s) 257
GluI GCNGC 2 cut(s) 366, 546
GsuI CTGGAG 1 cut(s) 559
HaeIII GGCC 2 cut(s) 137, 352
HapII CCGG 2 cut(s) 167, 268
HgaI GACGC 3 cut(s) 88, 111, 196
HhaI GCGC 1 cut(s) 258
Hin1I GRCGYC 2 cut(s) 80, 207
Hin1II CATG 5 cut(s) 429, 500, 575, 1025, 1079
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HincII GTYRAC 1 cut(s) 1090
HindII GTYRAC 1 cut(s) 1090
HinfI GANTC 6 cut(s) 158, 176, 182, 377, 419, 1062
HpaII CCGG 2 cut(s) 167, 268
HphI GGTGA 2 cut(s) 298, 396
Hpy166II GTNNAC 1 cut(s) 1090
Hpy188I TCNGA 4 cut(s) 181, 192, 294, 834
Hpy188III TCNNGA 2 cut(s) 99, 381
Hpy8I GTNNAC 1 cut(s) 1090
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 3 cut(s) 19, 634, 898
HpyCH4III ACNGT 2 cut(s) 24, 1014
HpyCH4V TGCA 5 cut(s) 284, 368, 679, 782, 1021
HpyF10VI GCNNNNNNNGC 2 cut(s) 542, 1027
HpyF3I CTNAG 2 cut(s) 555, 708
Hsp92I GRCGYC 2 cut(s) 80, 207
Hsp92II CATG 5 cut(s) 429, 500, 575, 1025, 1079
HspAI GCGC 1 cut(s) 256
Kzo9I GATC 4 cut(s) 198, 383, 913, 983
LguI GCTCTTC 1 cut(s) 221
Lsp1109I GCAGC 2 cut(s) 352, 532
LweI GCATC 2 cut(s) 571, 1008
MaeI CTAG 3 cut(s) 381, 932, 947
MaeIII GTNAC 2 cut(s) 919, 1079
MalI GATC 4 cut(s) 200, 385, 915, 985
MboI GATC 4 cut(s) 198, 383, 913, 983
MboII GAAGA 5 cut(s) 208, 258, 264, 512, 680
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 2 cut(s) 383, 983
MluCI AATT 5 cut(s) 61, 186, 300, 369, 653
MlyI GAGTC 2 cut(s) 170, 371
MmeI TCCRAC 1 cut(s) 1026
MnlI CCTC 4 cut(s) 127, 367, 514, 817
Mph1103I ATGCAT 1 cut(s) 1023
MroXI GAANNNNTTC 2 cut(s) 621, 1061
MseI TTAA 3 cut(s) 605, 648, 965
MslI CAYNNNNRTG 2 cut(s) 311, 427
MspI CCGG 2 cut(s) 167, 268
MspR9I CCNGG 2 cut(s) 168, 538
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 1 cut(s) 538
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 2 cut(s) 542, 1027
NciI CCSGG 1 cut(s) 168
NdeII GATC 4 cut(s) 198, 383, 913, 983
NlaIII CATG 5 cut(s) 429, 500, 575, 1025, 1079
NlaIV GGNNCC 1 cut(s) 138
NmuCI GTSAC 1 cut(s) 919
NsiI ATGCAT 1 cut(s) 1023
PaeR7I CTCGAG 1 cut(s) 97
PceI AGGCCT 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 221
PdmI GAANNNNTTC 2 cut(s) 621, 1061
PfeI GAWTC 4 cut(s) 158, 182, 419, 1062
PflFI GACNNNGTC 1 cut(s) 953
PflMI CCANNNNNTGG 1 cut(s) 1076
PinAI ACCGGT 1 cut(s) 267
PkrI GCNGC 2 cut(s) 367, 547
PleI GAGTC 2 cut(s) 170, 371
PpsI GAGTC 2 cut(s) 170, 371
Psp6I CCWGG 1 cut(s) 536
PspGI CCWGG 1 cut(s) 536
PspN4I GGNNCC 1 cut(s) 138
PspPI GGNCC 1 cut(s) 136
PsuI RGATCY 2 cut(s) 383, 983
PsyI GACNNNGTC 1 cut(s) 953
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 2 cut(s) 311, 427
SapI GCTCTTC 1 cut(s) 221
SaqAI TTAA 3 cut(s) 605, 648, 965
SatI GCNGC 2 cut(s) 366, 546
Sau3AI GATC 4 cut(s) 198, 383, 913, 983
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 2 cut(s) 170, 371
ScrFI CCNGG 2 cut(s) 168, 538
SfaNI GCATC 2 cut(s) 571, 1008
Sfr274I CTCGAG 1 cut(s) 97
SlaI CTCGAG 1 cut(s) 97
SmiMI CAYNNNNRTG 2 cut(s) 311, 427
SmlI CTYRAG 2 cut(s) 97, 329
SmoI CTYRAG 2 cut(s) 97, 329
Sse9I AATT 5 cut(s) 61, 186, 300, 369, 653
SseBI AGGCCT 1 cut(s) 352
SsiI CCGC 2 cut(s) 254, 977
SspI AATATT 1 cut(s) 873
SspMI CTAG 3 cut(s) 381, 932, 947
StuI AGGCCT 1 cut(s) 352
StyD4I CCNGG 2 cut(s) 166, 536
StyI CCWWGG 1 cut(s) 1070
TaaI ACNGT 2 cut(s) 24, 1014
TaqI TCGA 2 cut(s) 98, 201
TasI AATT 5 cut(s) 61, 186, 300, 369, 653
TfiI GAWTC 4 cut(s) 158, 182, 419, 1062
Tru1I TTAA 3 cut(s) 605, 648, 965
Tru9I TTAA 3 cut(s) 605, 648, 965
TscAI CASTG 2 cut(s) 49, 769
TseFI GTSAC 1 cut(s) 919
TseI GCWGC 2 cut(s) 365, 545
Tsp45I GTSAC 1 cut(s) 919
TspDTI ATGAA 7 cut(s) 141, 411, 453, 513, 630, 783, 870
TspGWI ACGGA 1 cut(s) 800
TspRI CASTG 2 cut(s) 49, 769
Tth111I GACNNNGTC 1 cut(s) 953
Van91I CCANNNNNTGG 1 cut(s) 1076
XapI RAATTY 2 cut(s) 300, 653
XbaI TCTAGA 1 cut(s) 380
XhoI CTCGAG 1 cut(s) 97
XmnI GAANNNNTTC 2 cut(s) 621, 1061
XspI CTAG 3 cut(s) 381, 932, 947
Zsp2I ATGCAT 1 cut(s) 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.