Rmu_sc0001209.1_g000011

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001209.1
OG0000105
Physical Location & Seq
Reverse (-)
34950 .. 39938
4989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001209.1_g000011.1.cds

Sequence Viewer

Length: 3948 bp
atgggtggtatcggtaagacaactattgcacaagtggtttttgaaagggtacaggctcagtttgaaggttgcagctttcttgagaatgttagagagcaaactgaaaagcaaggtgcagttcatttacaagagcaacttctttcgaatttgctgagatgtagtgtaaatgtacagaacactagaatgggaaaaaatataatgaggtataggctacggactaaaatggttcttatcattcttgatgatgtggaccaagaagaacaattggaagcattgtgtgatagggaatggtttggtccagggagtagaatcatcataacttctagagatgaacatttgctcagtgcagttgaaggagataaagtatataaggtgaatccattaactgatgctgaagctcttgagctctttggtatgaaagcctttaagaaagaccagttggagtgtaatgcagtggttaagtgcattggatggttagatcttccttcgtcttcttgtcctcctcctcctccatttcgtaaactgacatacgatgtgtctaccaaattagatcttccttcgtcttcttgtcctcctcctcctccatttcgtaaactgacatacgatgtgttcctcagtttcagaggtacagatacccccacatatttcacagaccatctttatgccgctctcaaacagaaaggaatatgcacatttagagatgatgaagaacttaacagaggggaatcaattggtccaaatctcttggaagcaattgaagagtcaagatatgtaattgtggtgttttctcagaactacgccgattcttcatggtgtttggatgagcttgcaaaagtagctgaatgcaggagagccatgggacaaacagtcctcccagtgttctaccatgttgatccatttgatgtacgaagacaaactggggggcactttgggaaagcatttgagaagcatcaagagcggtttggaggcaatctagacaaattggagaggtggaaagacgctcttgctgaagtaggcagtctctctggatggcatctgaatctgaaagatgggtacgaacccaaagtcattcatgaaattgttgataagattttcaccgagttaaatcaaataatcacaatatctgagggtttagttgggatggattctcacctgaatgaaatgctttcgtacttggacattgggtgtccggatgttcgtatcattgggatttgtggtatgggtggtatcggtaagacaactattgcacaagtggtttttgaaagggtacaggctcagtttgaaggttgcagctttcttgagaatgttagagagcaaactgaaaagcaaggtgcagttcatttacaagagcaacttgtttcgaatttgctgagatgtagtgtaaatgtacagaacactagaatgggaaaaaatataatgaggcataggctacgtacgaaaatggttcttatcattcttgatgatgtggaccaagaagaacaattggaagcattgtgtgatagggaatggtttggtccagggagtagaatcatcataacttctagagatgaacatttgctccatgcagttgaaggagataaagtatataaggtgaatccattaactgatgctgaagctcttgagctctttggtatgaaagcctttaagaaagaccagttggttgagaaagattttctcaagctatccaaggattttttgaaatatgctaatggccttccattagcgattaaagtattcgggtcgtctgtccgtggtaggagtgtaatgcagtggttaagtgcattggatggactaaaaaagaatcctcataaaggagttatggatgtgcttaaagtaagttttgatgcactagaagaaatggaaaaggttatttttttggacattgcatgtttttttaaaggagagagtatagatcgtgtaacaagaatactacatttatcgggcaatgttgttccagacattgatataaaagttcttgtggacaaatctctagtaactttggttggaagtaaattatggatgcatgacctggtacaacaattgggcaaagaaattgttttccaagaatgtcatgaagagcctggcaaacgtagcaggttgtggcttccgcaagaaatcatccatgtactcgagatgaataagggttcaaatgcagttcaagccatatccttagactttccaaaaacagaagagatacactgccactcagaggcattctctgtgatggacagacttcggtttctccaaatccgtaatgtgcatatgaaggaaggccccatttatcttcctggggaattaatagttctggaatggagtgggtatcctatagaaagtctaccacaaaattttgatcctaggcgattatgtgaacttaacctctgctacagcagcattgaacaactttggagtggaataaagaaattgccctgtttgacagttatcgatttaagctactcttgcaagttaatcaggaccccagattttacacatgctccagggcttgaaagattaattcttgaaggttgtacaagtttagaggacatccacccatccattgctcacctggagaggcttacttgcttgaatcttaaagattgcagaaatcttaaaagtcttccaagtaagcttgaaacgagatgccttcaaattcttaatctttcaggctgttcaaaagtcaaaaaaattccagattttgtgggatctatggaaggcttgttagaacttcttttggatgagactgctatagagcaaataactgtatcagttcgacttctgactggcctcattttattaaatttgagagattgcaaaaatcttaagagacttccaagagacattggcaatttacagtccctcaagcgtctcaatatctgtggatgctcaaaacttgaaactctaccagacagcttgggaaagatagactgcttggaagagcttgatgcggctggtggtagttccataagcaatataccatcctctatttcctgtttggaaaatcttgaagtgttatctctttgtggatgtaaagggatgtcaggcaacatgacaagatatcccctcagttttctgttgcccataagtggttcaggtggtttgcgttctttaacggtgctaaatctcagtgactgtaatcttcaggagggaagcttaccggaagattttggctgcttattccctttagtttcactaaatctaagcaaaaacaactttgatagtcttccaaaaagcatcagacagctttcaaagctttgcaacttgaatttggaaagttgtaagactcttcaaaaattgccagacctctcatcaagcttaaacttcagtttcggggcagacgatggttttttacgggaaaggctgtcatcagtcaatctgattaattgctccaaattggttgtcaatcaacgatgcaacaatatagcattgaaaatgctatggagatttcttcagggttttccttttgcaggaaatagatttgaaactataattcctggcagtgaaatctctgagctgttcagtgatcaaagtgtgggaccagtaataagcatggagctacctcgacaatggcacaagaataagtggatgggatatgcattttgtgcttgttttgttatccgtcgatatcttccagttccagctaaccggcttggtaaatggaagttcgggacccatagcactgcaaatggtcttcgattcgaagtgaagcctgagaatgtgggtatggctggctggtgtccttcctttgtctgcagccaagaattaggccaaattgagtccgaacacctttggctatcctttgtctctggtaattattttggaacagcatggcaagaaacctgtcataacattgattttacattcaaaacattaggcactggtatggaggtgaagaaatgtggagtccgtttgatatacgagcaagatttagatttgattggaatgaaaagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1315

Amino Acids

148.17

Weight (kDa)

6.08

Isoelectric Point (pI)

45.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2085
AccBSI CCGCTC 2 cut(s) 668, 958
AccI GTMKAC 2 cut(s) 539, 2335
AccIII TCCGGA 1 cut(s) 1189
AciI CCGC 4 cut(s) 666, 958, 2108, 2942
AclWI GGATC 3 cut(s) 887, 2345, 2707
AcsI RAATTY 7 cut(s) 145, 1363, 2344, 2646, 2682, 2794, 3259
AcuI CTGAAG 6 cut(s) 414, 1029, 1632, 3119, 3301, 3428
AfiI CCNNNNNNNGG 4 cut(s) 1811, 2209, 3080, 3461
AflII CTTAAG 1 cut(s) 2816
AhdI GACNNNNNGTC 1 cut(s) 866
AjnI CCWGG 8 cut(s) 298, 1516, 2028, 2080, 2287, 2494, 2562, 3487
AjuI GAANNNNNNNTTGG 4 cut(s) 422, 454, 1640, 1672
AloI GAACNNNNNNTCC 2 cut(s) 1542, 1574
Alw21I GWGCWC 2 cut(s) 408, 1626
Alw26I GTCTC 6 cut(s) 1025, 2729, 2815, 2826, 2867, 3802
AlwI GGATC 3 cut(s) 887, 2345, 2707
AlwNI CAGNNNCTG 1 cut(s) 3126
Ama87I CYCGRG 1 cut(s) 2129
Aor13HI TCCGGA 1 cut(s) 1189
AoxI GGCC 4 cut(s) 1711, 2272, 2779, 3760
ApeKI GCWGC 5 cut(s) 72, 1290, 2388, 3165, 3747
ApoI RAATTY 7 cut(s) 145, 1363, 2344, 2646, 2682, 2794, 3259
ArsI GACNNNNNNTTYG 4 cut(s) 1050, 1082, 3378, 3410
AseI ATTAAT 3 cut(s) 2297, 2510, 3375
AspA2I CCTAGG 1 cut(s) 2354
AspS9I GGNCC 9 cut(s) 250, 296, 734, 1468, 1514, 2273, 2472, 3530, 3663
AsuHPI GGTGA 6 cut(s) 385, 1087, 1142, 1603, 2552, 3895
AsuII TTCGAA 3 cut(s) 143, 1361, 3693
AvaI CYCGRG 1 cut(s) 2129
AvaII GGWCC 8 cut(s) 250, 296, 734, 1468, 1514, 2472, 3530, 3663
AvrII CCTAGG 1 cut(s) 2354
BaeGI GKGCMC 1 cut(s) 927
BanII GRGCYC 2 cut(s) 408, 1626
BbsI GAAGAC 6 cut(s) 483, 555, 916, 2606, 3209, 3677
Bbv12I GWGCWC 2 cut(s) 408, 1626
BbvI GCAGC 5 cut(s) 84, 1302, 2400, 3152, 3759
BciT130I CCWGG 8 cut(s) 300, 1518, 2030, 2082, 2289, 2496, 2564, 3489
BciVI GTATCC 1 cut(s) 2331
BclI TGATCA 1 cut(s) 3517
BcoDI GTCTC 6 cut(s) 1025, 2729, 2815, 2826, 2867, 3802
BfaI CTAG 8 cut(s) 180, 324, 974, 1398, 1542, 1850, 1991, 2355
BfmI CTRYAG 4 cut(s) 2325, 2383, 2742, 3745
BfrI CTTAAG 1 cut(s) 2816
BfuAI ACCTGC 1 cut(s) 2085
BfuI GTATCC 1 cut(s) 2331
BglII AGATCT 2 cut(s) 478, 550
BisI GCNGC 7 cut(s) 73, 666, 1291, 2389, 2943, 3166, 3748
BlnI CCTAGG 1 cut(s) 2354
BlsI GCNGC 7 cut(s) 74, 667, 1292, 2390, 2944, 3167, 3749
Bme1390I CCNGG 8 cut(s) 300, 1518, 2030, 2082, 2289, 2496, 2564, 3489
Bme18I GGWCC 8 cut(s) 250, 296, 734, 1468, 1514, 2472, 3530, 3663
BmeRI GACNNNNNGTC 1 cut(s) 866
BmeT110I CYCGRG 1 cut(s) 2129
BmgT120I GGNCC 9 cut(s) 250, 296, 734, 1468, 1514, 2273, 2472, 3530, 3663
BmiI GGNNCC 5 cut(s) 2275, 2474, 3531, 3664, 3665
BmrFI CCNGG 8 cut(s) 300, 1518, 2030, 2082, 2289, 2496, 2564, 3489
BmrI ACTGGG 2 cut(s) 869, 927
BmuI ACTGGG 2 cut(s) 869, 927
BpiI GAAGAC 6 cut(s) 483, 555, 916, 2606, 3209, 3677
BplI GAGNNNNNCTC 2 cut(s) 2201, 2233
BpmI CTGGAG 2 cut(s) 2478, 2585
Bpu14I TTCGAA 3 cut(s) 143, 1361, 3693
BpuEI CTTGAG 6 cut(s) 101, 422, 1319, 1640, 1661, 2840
Bsa29I ATCGAT 1 cut(s) 2442
BsaAI YACGTR 1 cut(s) 1433
BsaJI CCNNGG 8 cut(s) 299, 855, 1517, 1686, 1750, 2288, 2354, 2495
BsaWI WCCGGW 2 cut(s) 1189, 3151
BsaXI ACNNNNNCTCC 8 cut(s) 348, 378, 1542, 1566, 1572, 1596, 2476, 2506
Bsc4I CCNNNNNNNGG 4 cut(s) 1811, 2209, 3080, 3461
Bse118I RCCGGY 1 cut(s) 3639
Bse1I ACTGG 8 cut(s) 436, 875, 922, 1654, 2782, 3533, 3626, 3877
Bse3DI GCAATG 3 cut(s) 1881, 1951, 2553
BseAI TCCGGA 1 cut(s) 1189
BseBI CCWGG 8 cut(s) 300, 1518, 2030, 2082, 2289, 2496, 2564, 3489
BseCI ATCGAT 1 cut(s) 2442
BseDI CCNNGG 8 cut(s) 299, 855, 1517, 1686, 1750, 2288, 2354, 2495
BseLI CCNNNNNNNGG 4 cut(s) 1811, 2209, 3080, 3461
BseMI GCAATG 3 cut(s) 1881, 1951, 2553
BseNI ACTGG 8 cut(s) 436, 875, 922, 1654, 2782, 3533, 3626, 3877
BseRI GAGGAG 6 cut(s) 492, 495, 498, 564, 567, 570
BseSI GKGCMC 1 cut(s) 927
BseXI GCAGC 5 cut(s) 84, 1302, 2400, 3152, 3759
BsgI GTGCAG 3 cut(s) 135, 366, 1353
BshFI GGCC 4 cut(s) 1713, 2274, 2781, 3762
BshVI ATCGAT 1 cut(s) 2442
BsiHKAI GWGCWC 2 cut(s) 408, 1626
BsiHKCI CYCGRG 1 cut(s) 2129
BsiSI CCGG 3 cut(s) 1190, 3152, 3640
BsiWI CGTACG 1 cut(s) 1433
BslFI GGGAC 4 cut(s) 873, 2836, 3543, 3676
BslI CCNNNNNNNGG 4 cut(s) 1811, 2209, 3080, 3461
BsmAI GTCTC 6 cut(s) 1025, 2729, 2815, 2826, 2867, 3802
BsmBI CGTCTC 1 cut(s) 2867
BsmFI GGGAC 4 cut(s) 873, 2836, 3543, 3676
BsmI GAATGC 2 cut(s) 848, 2213
BsnI GGCC 4 cut(s) 1713, 2274, 2781, 3762
BsoBI CYCGRG 1 cut(s) 2129
Bsp119I TTCGAA 3 cut(s) 143, 1361, 3693
Bsp1286I GDGCHC 3 cut(s) 408, 927, 1626
Bsp13I TCCGGA 1 cut(s) 1189
Bsp1407I TGTACA 3 cut(s) 169, 1387, 2525
Bsp143I GATC 7 cut(s) 478, 550, 892, 1912, 2350, 2699, 3517
Bsp19I CCATGG 1 cut(s) 855
BspACI CCGC 4 cut(s) 666, 958, 2108, 2942
BspANI GGCC 4 cut(s) 1713, 2274, 2781, 3762
BspDI ATCGAT 1 cut(s) 2442
BspEI TCCGGA 1 cut(s) 1189
BspHI TCATGA 2 cut(s) 1072, 2071
BspLI GGNNCC 5 cut(s) 2275, 2474, 3531, 3664, 3665
BspMAI CTGCAG 1 cut(s) 3749
BspMI ACCTGC 1 cut(s) 2085
BspPI GGATC 3 cut(s) 887, 2345, 2707
BspQI GCTCTTC 2 cut(s) 2070, 2925
BspT104I TTCGAA 3 cut(s) 143, 1361, 3693
BspTI CTTAAG 1 cut(s) 2816
BsrBI CCGCTC 2 cut(s) 668, 958
BsrDI GCAATG 3 cut(s) 1881, 1951, 2553
BsrFI RCCGGY 1 cut(s) 3639
BsrGI TGTACA 3 cut(s) 169, 1387, 2525
BsrI ACTGG 8 cut(s) 436, 875, 922, 1654, 2782, 3533, 3626, 3877
BssAI RCCGGY 1 cut(s) 3639
BssECI CCNNGG 8 cut(s) 299, 855, 1517, 1686, 1750, 2288, 2354, 2495
BssMI GATC 7 cut(s) 478, 550, 892, 1912, 2350, 2699, 3517
BssT1I CCWWGG 3 cut(s) 855, 1686, 2354
Bst2UI CCWGG 8 cut(s) 300, 1518, 2030, 2082, 2289, 2496, 2564, 3489
Bst4CI ACNGT 6 cut(s) 868, 2437, 2758, 2850, 3109, 3128
Bst6I CTCTTC 5 cut(s) 753, 2070, 2184, 2925, 3285
BstAFI CTTAAG 1 cut(s) 2816
BstAPI GCANNNNNTGC 1 cut(s) 3596
BstAUI TGTACA 3 cut(s) 169, 1387, 2525
BstBAI YACGTR 1 cut(s) 1433
BstBI TTCGAA 3 cut(s) 143, 1361, 3693
BstC8I GCNNGC 2 cut(s) 828, 3724
BstDSI CCRYGG 2 cut(s) 855, 1750
BstENI CCTNNNNNAGG 2 cut(s) 1809, 3459
BstKTI GATC 7 cut(s) 481, 553, 895, 1915, 2353, 2702, 3520
BstMAI GTCTC 6 cut(s) 1025, 2729, 2815, 2826, 2867, 3802
BstMBI GATC 7 cut(s) 478, 550, 892, 1912, 2350, 2699, 3517
BstMWI GCNNNNNNNGC 8 cut(s) 836, 955, 2091, 2159, 2388, 2457, 3244, 3596
BstNI CCWGG 8 cut(s) 300, 1518, 2030, 2082, 2289, 2496, 2564, 3489
BstNSI RCATGY 2 cut(s) 1890, 2492
BstSCI CCNGG 8 cut(s) 298, 1516, 2028, 2080, 2287, 2494, 2562, 3487
BstSFI CTRYAG 4 cut(s) 2325, 2383, 2742, 3745
BstSLI GKGCMC 1 cut(s) 927
BstSNI TACGTA 1 cut(s) 1433
BstV1I GCAGC 5 cut(s) 84, 1302, 2400, 3152, 3759
BstV2I GAAGAC 6 cut(s) 483, 555, 916, 2606, 3209, 3677
BstX2I RGATCY 3 cut(s) 478, 550, 2699
BstYI RGATCY 3 cut(s) 478, 550, 2699
Bsu15I ATCGAT 1 cut(s) 2442
BsuI GTATCC 1 cut(s) 2331
BsuRI GGCC 4 cut(s) 1713, 2274, 2781, 3762
BsuTUI ATCGAT 1 cut(s) 2442
BtgI CCRYGG 2 cut(s) 855, 1750
BtsI GCAGTG 5 cut(s) 459, 1775, 2197, 3499, 3672
BveI ACCTGC 1 cut(s) 2085
Cac8I GCNNGC 2 cut(s) 828, 3724
CaiI CAGNNNCTG 1 cut(s) 3126
CciI TCATGA 2 cut(s) 1072, 2071
Cfr10I RCCGGY 1 cut(s) 3639
Cfr13I GGNCC 9 cut(s) 250, 296, 734, 1468, 1514, 2273, 2472, 3530, 3663
ClaI ATCGAT 1 cut(s) 2442
CseI GACGC 2 cut(s) 1007, 2849
CsiI ACCWGGT 1 cut(s) 2028
CspCI CAANNNNNGTGG 2 cut(s) 2854, 2889
DpnI GATC 7 cut(s) 480, 552, 894, 1914, 2352, 2701, 3519
DpnII GATC 7 cut(s) 478, 550, 892, 1912, 2350, 2699, 3517
DraI TTTAAA 1 cut(s) 1897
DriI GACNNNNNGTC 1 cut(s) 866
Eam1104I CTCTTC 5 cut(s) 753, 2070, 2184, 2925, 3285
Eam1105I GACNNNNNGTC 1 cut(s) 866
EarI CTCTTC 5 cut(s) 753, 2070, 2184, 2925, 3285
Ecl136II GAGCTC 2 cut(s) 406, 1624
Eco105I TACGTA 1 cut(s) 1433
Eco130I CCWWGG 3 cut(s) 855, 1686, 2354
Eco24I GRGCYC 2 cut(s) 408, 1626
Eco32I GATATC 2 cut(s) 3053, 3620
Eco47I GGWCC 8 cut(s) 250, 296, 734, 1468, 1514, 2472, 3530, 3663
Eco53kI GAGCTC 2 cut(s) 406, 1624
Eco57I CTGAAG 6 cut(s) 414, 1029, 1632, 3119, 3301, 3428
Eco88I CYCGRG 1 cut(s) 2129
EcoICRI GAGCTC 2 cut(s) 406, 1624
EcoNI CCTNNNNNAGG 2 cut(s) 1809, 3459
EcoO109I RGGNCCY 3 cut(s) 2273, 2472, 3663
EcoRII CCWGG 8 cut(s) 298, 1516, 2028, 2080, 2287, 2494, 2562, 3487
EcoRV GATATC 2 cut(s) 3053, 3620
EcoT14I CCWWGG 3 cut(s) 855, 1686, 2354
EcoT22I ATGCAT 2 cut(s) 2025, 3592
EcoT38I GRGCYC 2 cut(s) 408, 1626
ErhI CCWWGG 3 cut(s) 855, 1686, 2354
Esp3I CGTCTC 1 cut(s) 2867
FalI AAGNNNNNCTT 8 cut(s) 120, 152, 987, 1019, 1338, 1370, 2440, 2472
FaqI GGGAC 4 cut(s) 873, 2836, 3543, 3676
FauNDI CATATG 1 cut(s) 2262
FbaI TGATCA 1 cut(s) 3517
FblI GTMKAC 2 cut(s) 539, 2335
Fnu4HI GCNGC 7 cut(s) 73, 666, 1291, 2389, 2943, 3166, 3748
FriOI GRGCYC 2 cut(s) 408, 1626
Fsp4HI GCNGC 7 cut(s) 73, 666, 1291, 2389, 2943, 3166, 3748
FspBI CTAG 8 cut(s) 180, 324, 974, 1398, 1542, 1850, 1991, 2355
GluI GCNGC 7 cut(s) 73, 666, 1291, 2389, 2943, 3166, 3748
GsuI CTGGAG 2 cut(s) 2478, 2585
HaeIII GGCC 4 cut(s) 1713, 2274, 2781, 3762
HapII CCGG 3 cut(s) 1190, 3152, 3640
HgaI GACGC 2 cut(s) 1007, 2849
HindIII AAGCTT 4 cut(s) 2624, 3145, 3245, 3307
HpaII CCGG 3 cut(s) 1190, 3152, 3640
HphI GGTGA 6 cut(s) 385, 1087, 1142, 1603, 2552, 3895
Hpy166II GTNNAC 8 cut(s) 250, 521, 540, 593, 1468, 1981, 2336, 2369
Hpy8I GTNNAC 8 cut(s) 250, 521, 540, 593, 1468, 1981, 2336, 2369
Hpy99I CGWCG 1 cut(s) 3618
HpyCH4III ACNGT 6 cut(s) 868, 2437, 2758, 2850, 3109, 3128
HpyCH4IV ACGT 2 cut(s) 1432, 2089
HpyF10VI GCNNNNNNNGC 8 cut(s) 836, 955, 2091, 2159, 2388, 2457, 3244, 3596
HpySE526I ACGT 2 cut(s) 1432, 2089
KflI GGGWCCC 1 cut(s) 3663
Kpn2I TCCGGA 1 cut(s) 1189
Ksp22I TGATCA 1 cut(s) 3517
Kzo9I GATC 7 cut(s) 478, 550, 892, 1912, 2350, 2699, 3517
LguI GCTCTTC 2 cut(s) 2070, 2925
LmnI GCTCC 4 cut(s) 1563, 2497, 3386, 3547
Lsp1109I GCAGC 5 cut(s) 84, 1302, 2400, 3152, 3759
MabI ACCWGGT 1 cut(s) 2028
MaeI CTAG 8 cut(s) 180, 324, 974, 1398, 1542, 1850, 1991, 2355
MaeII ACGT 2 cut(s) 1432, 2089
MaeIII GTNAC 3 cut(s) 1918, 1993, 3122
MalI GATC 7 cut(s) 480, 552, 894, 1914, 2352, 2701, 3519
MbiI CCGCTC 2 cut(s) 668, 958
MboI GATC 7 cut(s) 478, 550, 892, 1912, 2350, 2699, 3517
MfeI CAATTG 5 cut(s) 263, 729, 753, 1481, 2039
MflI RGATCY 3 cut(s) 478, 550, 2699
MhlI GDGCHC 3 cut(s) 408, 927, 1626
MlyI GAGTC 4 cut(s) 770, 3271, 3779, 3906
MmeI TCCRAC 2 cut(s) 420, 1984
Mph1103I ATGCAT 2 cut(s) 2025, 3592
MroI TCCGGA 1 cut(s) 1189
MslI CAYNNNNRTG 5 cut(s) 182, 660, 1155, 1400, 3875
MspCI CTTAAG 1 cut(s) 2816
MspI CCGG 3 cut(s) 1190, 3152, 3640
MspR9I CCNGG 8 cut(s) 300, 1518, 2030, 2082, 2289, 2496, 2564, 3489
MunI CAATTG 5 cut(s) 263, 729, 753, 1481, 2039
Mva1269I GAATGC 2 cut(s) 848, 2213
MvaI CCWGG 8 cut(s) 300, 1518, 2030, 2082, 2289, 2496, 2564, 3489
MwoI GCNNNNNNNGC 8 cut(s) 836, 955, 2091, 2159, 2388, 2457, 3244, 3596
NcoI CCATGG 1 cut(s) 855
NdeI CATATG 1 cut(s) 2262
NdeII GATC 7 cut(s) 478, 550, 892, 1912, 2350, 2699, 3517
NlaIV GGNNCC 5 cut(s) 2275, 2474, 3531, 3664, 3665
NmuCI GTSAC 1 cut(s) 3122
NsiI ATGCAT 2 cut(s) 2025, 3592
NspI RCATGY 2 cut(s) 1890, 2492
NspV TTCGAA 3 cut(s) 143, 1361, 3693
PaeR7I CTCGAG 1 cut(s) 2129
PagI TCATGA 2 cut(s) 1072, 2071
PciSI GCTCTTC 2 cut(s) 2070, 2925
PcsI WCGNNNNNNNCGW 1 cut(s) 3330
PctI GAATGC 2 cut(s) 848, 2213
Pfl23II CGTACG 1 cut(s) 1433
PkrI GCNGC 7 cut(s) 74, 667, 1292, 2390, 2944, 3167, 3749
PleI GAGTC 4 cut(s) 769, 3271, 3778, 3905
PpsI GAGTC 4 cut(s) 769, 3271, 3778, 3905
Ppu21I YACGTR 1 cut(s) 1433
PpuMI RGGWCCY 2 cut(s) 2472, 3663
PshBI ATTAAT 3 cut(s) 2297, 2510, 3375
Psp124BI GAGCTC 2 cut(s) 408, 1626
Psp5II RGGWCCY 2 cut(s) 2472, 3663
Psp6I CCWGG 8 cut(s) 298, 1516, 2028, 2080, 2287, 2494, 2562, 3487
PspGI CCWGG 8 cut(s) 298, 1516, 2028, 2080, 2287, 2494, 2562, 3487
PspLI CGTACG 1 cut(s) 1433
PspN4I GGNNCC 5 cut(s) 2275, 2474, 3531, 3664, 3665
PspPI GGNCC 9 cut(s) 250, 296, 734, 1468, 1514, 2273, 2472, 3530, 3663
PspPPI RGGWCCY 2 cut(s) 2472, 3663
PstI CTGCAG 1 cut(s) 3749
PstNI CAGNNNCTG 1 cut(s) 3126
PsuI RGATCY 3 cut(s) 478, 550, 2699
RseI CAYNNNNRTG 5 cut(s) 182, 660, 1155, 1400, 3875
SacI GAGCTC 2 cut(s) 408, 1626
SapI GCTCTTC 2 cut(s) 2070, 2925
SatI GCNGC 7 cut(s) 73, 666, 1291, 2389, 2943, 3166, 3748
Sau3AI GATC 7 cut(s) 478, 550, 892, 1912, 2350, 2699, 3517
Sau96I GGNCC 9 cut(s) 250, 296, 734, 1468, 1514, 2273, 2472, 3530, 3663
SchI GAGTC 4 cut(s) 770, 3271, 3779, 3906
ScrFI CCNGG 8 cut(s) 300, 1518, 2030, 2082, 2289, 2496, 2564, 3489
SduI GDGCHC 3 cut(s) 408, 927, 1626
SexAI ACCWGGT 1 cut(s) 2028
SfcI CTRYAG 4 cut(s) 2325, 2383, 2742, 3745
Sfr274I CTCGAG 1 cut(s) 2129
SfuI TTCGAA 3 cut(s) 143, 1361, 3693
SinI GGWCC 8 cut(s) 250, 296, 734, 1468, 1514, 2472, 3530, 3663
SlaI CTCGAG 1 cut(s) 2129
SmiMI CAYNNNNRTG 5 cut(s) 182, 660, 1155, 1400, 3875
SmlI CTYRAG 8 cut(s) 80, 401, 1298, 1619, 1676, 2129, 2816, 2855
SmoI CTYRAG 8 cut(s) 80, 401, 1298, 1619, 1676, 2129, 2816, 2855
SnaBI TACGTA 1 cut(s) 1433
SsiI CCGC 4 cut(s) 666, 958, 2108, 2942
SspMI CTAG 8 cut(s) 180, 324, 974, 1398, 1542, 1850, 1991, 2355
SstI GAGCTC 2 cut(s) 408, 1626
StyD4I CCNGG 8 cut(s) 298, 1516, 2028, 2080, 2287, 2494, 2562, 3487
StyI CCWWGG 3 cut(s) 855, 1686, 2354
TaaI ACNGT 6 cut(s) 868, 2437, 2758, 2850, 3109, 3128
TaiI ACGT 2 cut(s) 1435, 2092
TaqI TCGA 9 cut(s) 143, 1361, 2130, 2442, 2767, 3556, 3616, 3688, 3693
TatI WGTACW 4 cut(s) 169, 1387, 2125, 2525
TauI GCSGC 2 cut(s) 668, 2945
TseFI GTSAC 1 cut(s) 3122
TseI GCWGC 5 cut(s) 72, 1290, 2388, 3165, 3747
Tsp45I GTSAC 1 cut(s) 3122
TspGWI ACGGA 5 cut(s) 229, 1739, 2240, 3602, 3890
Vha464I CTTAAG 1 cut(s) 2816
VpaK11BI GGWCC 8 cut(s) 250, 296, 734, 1468, 1514, 2472, 3530, 3663
VspI ATTAAT 3 cut(s) 2297, 2510, 3375
XagI CCTNNNNNAGG 2 cut(s) 1809, 3459
XapI RAATTY 7 cut(s) 145, 1363, 2344, 2646, 2682, 2794, 3259
XbaI TCTAGA 3 cut(s) 323, 973, 1541
XceI RCATGY 2 cut(s) 1890, 2492
XcmI CCANNNNNNNNNTGG 1 cut(s) 2560
XhoI CTCGAG 1 cut(s) 2129
XmaJI CCTAGG 1 cut(s) 2354
XmiI GTMKAC 2 cut(s) 539, 2335
XspI CTAG 8 cut(s) 180, 324, 974, 1398, 1542, 1850, 1991, 2355
Zsp2I ATGCAT 2 cut(s) 2025, 3592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.