RLG00000030301

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000105
Physical Location & Seq
Reverse (-)
59263062 .. 59266674
3613 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030301

Sequence Viewer

Length: 2763 bp
ATGGTGATCATTTCCGTGAACAACACAACAGGCGAGTCTGATTCAATTCCGATTAGGAGATCGATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTGTTTCTAAGTTTCAGGGGGGAAGATACCCGCAAGGGTTTTACTGGTCGCCTGTATTCTGCACTAAATCAGAGAGGAATTCTCACCTTTATGGATGACGCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAGAGGCAATTGAGGATTCTAGATCTGCGATTGTCATTCTCTCATGGAACTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGATGGGCCAACAAGTCCTTCCCGTCTTCTACTGGGTGGATCCTTCAGATGTGCGGCACCAAAGGAGATGGTTTGAGCTCAAACATTGGGAACCCAAAGTAGAAGTAAGGGAGCATGAAGAAGTTTATCGGAAGAGTGAGGACAGACTAACTCCTTGGAGAGCTGCTTTGACTGAGGTCGCCAATCTCTCTGGCTGGGATGCAAAGAATACTCCATCTTATGCAGAACTAATTGACGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCGTCGATGATTTATTAAGCAATTACATATATCTGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGGATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAACCAATGGCCTTGTTTCTCTACAAGAAAAACTTCTTTCTAGAATCCTAATGGCAAAAATTGAAAATATAGAGGATGAATATACAGGAGCTGCTATGATAGAAAGGCGGCTGTGTAGAAGAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGAAGTAGAATCATCATAACCACCACAGATATTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTATTTGGTTAAACTATGCTGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATGTCCCCCACCAGAACATTATTTGCATCTGTGCCACCATATTTTAGGGTATGCTCAGGGGCTTCCATTGGCGGTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCACTGATAAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTAACGTGCTTCGGGTTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAGACCCTGTCATTGGTTTAGATGTACTTGCTGATAGATCTCTCATAACTATCTCCAACAACAAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAACAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAGACATAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCACAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGCGTCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCTAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCCAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGAAACGTTTGTTGGTGCTTAGTCTTGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGTCATTGAATCTAAGCAACTGCAGAAATCTTGTATGTCTTCCAAGCACCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTTGGCAAACATCAGGTAAATGTGGGGGAGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATCTCATCTGCCCGTGATCGCATAAAATATGTGAGAGGTTCAGTCATTCATGGATCCGAAGTAGTTTGGGGACCTGTAAATAAGTTCTTGCCTTGTGGATTGGTGCAAAAAGTGGATACAGAGCCAATGAGTTTCCACTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGGCCACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGGAACAATTTTGTTCACCTTCCTTCAGGCATTAGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGGCCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGTACTCCCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

921

Amino Acids

104.29

Weight (kDa)

6.89

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 32 - 211 2.3e-49 TIR domain
TIR_2 PF13676 35 - 146 1.8e-15 TIR domain
NB-ARC PF00931 236 - 391 5.9e-19 NB-ARC domain
AAA_22 PF13401 239 - 338 7.5e-06 AAA domain
NACHT PF05729 241 - 337 3.4e-07 NACHT domain
WHD_ROQ1 PF23282 458 - 529 2e-23 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 626 - 711 7.6e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 637 - 706 4e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 815
AccB1I GGYRCC 1 cut(s) 405
AccI GTMKAC 2 cut(s) 620, 2578
AccII CGCG 1 cut(s) 666
AciI CCGC 6 cut(s) 133, 403, 664, 739, 946, 1244
AclI AACGTT 1 cut(s) 1951
AclWI GGATC 8 cut(s) 383, 396, 721, 734, 996, 1440, 2253, 2266
AcoI YGGCCR 1 cut(s) 2428
AcsI RAATTY 6 cut(s) 180, 783, 1660, 1771, 2423, 2635
AcuI CTGAAG 5 cut(s) 378, 1155, 1667, 2472, 2750
AcyI GRCGYC 1 cut(s) 68
AfaI GTAC 3 cut(s) 1491, 2605, 2751
AfiI CCNNNNNNNGG 2 cut(s) 1478, 2354
AjnI CCWGG 2 cut(s) 1039, 1597
AjuI GAANNNNNNNTTGG 6 cut(s) 351, 383, 1940, 1972, 2510, 2542
AluBI AGCT 9 cut(s) 208, 427, 512, 703, 770, 929, 1465, 1813, 2512
AluI AGCT 9 cut(s) 208, 427, 512, 703, 770, 929, 1465, 1813, 2512
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 7 cut(s) 1590, 1626, 1645, 1706, 1850, 1970, 2536
AlwI GGATC 8 cut(s) 383, 396, 721, 734, 996, 1440, 2253, 2266
AlwNI CAGNNNCTG 1 cut(s) 929
Ama87I CYCGRG 3 cut(s) 765, 1847, 1928
AoxI GGCC 6 cut(s) 230, 355, 847, 2020, 2428, 2560
ApeKI GCWGC 3 cut(s) 512, 700, 929
ApoI RAATTY 6 cut(s) 180, 783, 1660, 1771, 2423, 2635
AseI ATTAAT 1 cut(s) 1892
Asp700I GAANNNNTTC 5 cut(s) 658, 870, 1140, 1572, 2735
AspS9I GGNCC 3 cut(s) 355, 1037, 2276
AsuHPI GGTGA 5 cut(s) 16, 178, 2414, 2458, 2471
AvaI CYCGRG 3 cut(s) 765, 1847, 1928
AvaII GGWCC 2 cut(s) 1037, 2276
BalI TGGCCA 1 cut(s) 2430
BamHI GGATCC 3 cut(s) 388, 726, 2258
BanI GGYRCC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 429
BbsI GAAGAC 4 cut(s) 367, 1635, 2057, 2738
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 3 cut(s) 499, 712, 916
BceAI ACGGC 1 cut(s) 2027
BcgI CGANNNNNNTGC 2 cut(s) 1828, 1862
BciT130I CCWGG 2 cut(s) 1041, 1599
BciVI GTATCC 1 cut(s) 2314
BclI TGATCA 1 cut(s) 6
BcoDI GTCTC 7 cut(s) 1590, 1626, 1645, 1706, 1850, 1970, 2536
BfaI CTAG 6 cut(s) 261, 879, 1451, 1466, 1860, 2582
BfmI CTRYAG 2 cut(s) 698, 2047
BfuAI ACCTGC 1 cut(s) 815
BfuI GTATCC 1 cut(s) 2314
BglII AGATCT 3 cut(s) 263, 1502, 1753
BisI GCNGC 5 cut(s) 404, 513, 701, 930, 947
BlsI GCNGC 5 cut(s) 405, 514, 702, 931, 948
Bme1390I CCNGG 2 cut(s) 1041, 1599
Bme18I GGWCC 2 cut(s) 1037, 2276
BmeT110I CYCGRG 3 cut(s) 765, 1847, 1928
BmgT120I GGNCC 3 cut(s) 355, 1037, 2276
BmiI GGNNCC 6 cut(s) 390, 407, 441, 728, 2260, 2277
BmrFI CCNGG 2 cut(s) 1041, 1599
BmrI ACTGGG 1 cut(s) 391
BmsI GCATC 8 cut(s) 538, 1090, 1207, 1527, 2108, 2617, 2639, 2693
BmuI ACTGGG 1 cut(s) 391
BoxI GACNNNNGTC 1 cut(s) 524
BpiI GAAGAC 4 cut(s) 367, 1635, 2057, 2738
BplI GAGNNNNNCTC 2 cut(s) 168, 200
Bpu10I CCTNAGC 1 cut(s) 1227
BpuEI CTTGAG 3 cut(s) 230, 1763, 2534
Bsa29I ATCGAT 1 cut(s) 62
BsaBI GATNNNNATC 2 cut(s) 987, 1431
BsaHI GRCGYC 1 cut(s) 68
BsaI GGTCTC 2 cut(s) 1626, 1706
BsaJI CCNNGG 3 cut(s) 503, 730, 1795
Bsc4I CCNNNNNNNGG 2 cut(s) 1478, 2354
Bse1I ACTGG 5 cut(s) 151, 386, 1034, 1374, 2023
Bse8I GATNNNNATC 2 cut(s) 987, 1431
BseBI CCWGG 2 cut(s) 1041, 1599
BseCI ATCGAT 1 cut(s) 62
BseDI CCNNGG 3 cut(s) 503, 730, 1795
BseGI GGATG 8 cut(s) 202, 322, 553, 919, 1378, 1769, 2123, 2708
BseJI GATNNNNATC 2 cut(s) 987, 1431
BseLI CCNNNNNNNGG 2 cut(s) 1478, 2354
BseMII CTCAG 3 cut(s) 513, 1241, 2375
BseNI ACTGG 5 cut(s) 151, 386, 1034, 1374, 2023
BseXI GCAGC 3 cut(s) 499, 712, 916
BseYI CCCAGC 2 cut(s) 543, 1563
BsgI GTGCAG 1 cut(s) 147
Bsh1236I CGCG 1 cut(s) 666
BshFI GGCC 6 cut(s) 232, 357, 849, 2022, 2430, 2562
BshNI GGYRCC 1 cut(s) 405
BshVI ATCGAT 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 3 cut(s) 765, 1847, 1928
BslFI GGGAC 2 cut(s) 1161, 2289
BslI CCNNNNNNNGG 2 cut(s) 1478, 2354
BsmAI GTCTC 7 cut(s) 1590, 1626, 1645, 1706, 1850, 1970, 2536
BsmFI GGGAC 2 cut(s) 1161, 2289
BsnI GGCC 6 cut(s) 232, 357, 849, 2022, 2430, 2562
Bso31I GGTCTC 2 cut(s) 1626, 1706
BsoBI CYCGRG 3 cut(s) 765, 1847, 1928
Bsp1286I GDGCHC 1 cut(s) 429
Bsp19I CCATGG 1 cut(s) 730
BspACI CCGC 6 cut(s) 133, 403, 664, 739, 946, 1244
BspANI GGCC 6 cut(s) 232, 357, 849, 2022, 2430, 2562
BspCNI CTCAG 3 cut(s) 514, 1240, 2376
BspDI ATCGAT 1 cut(s) 62
BspFNI CGCG 1 cut(s) 666
BspHI TCATGA 1 cut(s) 1624
BspLI GGNNCC 6 cut(s) 390, 407, 441, 728, 2260, 2277
BspMAI CTGCAG 2 cut(s) 702, 2051
BspMI ACCTGC 1 cut(s) 815
BspPI GGATC 8 cut(s) 383, 396, 721, 734, 996, 1440, 2253, 2266
BspT107I GGYRCC 1 cut(s) 405
BspTNI GGTCTC 2 cut(s) 1626, 1706
BsrI ACTGG 5 cut(s) 151, 386, 1034, 1374, 2023
BssECI CCNNGG 3 cut(s) 503, 730, 1795
BssNAI GTATAC 2 cut(s) 621, 2579
BssNI GRCGYC 1 cut(s) 68
BssT1I CCWWGG 3 cut(s) 503, 730, 1795
Bst1107I GTATAC 2 cut(s) 621, 2579
Bst2UI CCWGG 2 cut(s) 1041, 1599
Bst4CI ACNGT 3 cut(s) 1533, 1584, 2165
Bst6I CTCTTC 3 cut(s) 82, 476, 1643
BstACI GRCGYC 1 cut(s) 68
BstAPI GCANNNNNTGC 1 cut(s) 1546
BstC8I GCNNGC 3 cut(s) 737, 1408, 1463
BstDEI CTNAG 8 cut(s) 110, 522, 1227, 1964, 2039, 2384, 2459, 2508
BstDSI CCRYGG 1 cut(s) 730
BstEII GGTNACC 1 cut(s) 2446
BstF5I GGATG 8 cut(s) 202, 322, 553, 919, 1378, 1769, 2123, 2708
BstFNI CGCG 1 cut(s) 666
BstMAI GTCTC 7 cut(s) 1590, 1626, 1645, 1706, 1850, 1970, 2536
BstMWI GCNNNNNNNGC 2 cut(s) 1546, 2509
BstNI CCWGG 2 cut(s) 1041, 1599
BstNSI RCATGY 1 cut(s) 739
BstPAI GACNNNNGTC 1 cut(s) 524
BstPI GGTNACC 1 cut(s) 2446
BstSCI CCNGG 2 cut(s) 1039, 1597
BstSFI CTRYAG 2 cut(s) 698, 2047
BstUI CGCG 1 cut(s) 666
BstV1I GCAGC 3 cut(s) 499, 712, 916
BstV2I GAAGAC 4 cut(s) 367, 1635, 2057, 2738
BstX2I RGATCY 6 cut(s) 263, 388, 726, 1502, 1753, 2258
BstYI RGATCY 6 cut(s) 263, 388, 726, 1502, 1753, 2258
BstZ17I GTATAC 2 cut(s) 621, 2579
Bsu15I ATCGAT 1 cut(s) 62
BsuI GTATCC 1 cut(s) 2314
BsuRI GGCC 6 cut(s) 232, 357, 849, 2022, 2430, 2562
BsuTUI ATCGAT 1 cut(s) 62
BtgI CCRYGG 1 cut(s) 730
BtsCI GGATG 8 cut(s) 202, 322, 553, 919, 1378, 1769, 2123, 2708
BtsIMutI CAGTG 2 cut(s) 1281, 2609
BveI ACCTGC 1 cut(s) 815
Cac8I GCNNGC 3 cut(s) 737, 1408, 1463
CaiI CAGNNNCTG 1 cut(s) 929
CciI TCATGA 1 cut(s) 1624
Cfr13I GGNCC 3 cut(s) 355, 1037, 2276
ClaI ATCGAT 1 cut(s) 62
CseI GACGC 4 cut(s) 57, 209, 655, 1771
Csp6I GTAC 3 cut(s) 1490, 2604, 2750
CviQI GTAC 3 cut(s) 1490, 2604, 2750
DdeI CTNAG 8 cut(s) 110, 522, 1227, 1964, 2039, 2384, 2459, 2508
EaeI YGGCCR 1 cut(s) 2428
Eam1104I CTCTTC 3 cut(s) 82, 476, 1643
EarI CTCTTC 3 cut(s) 82, 476, 1643
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 3 cut(s) 503, 730, 1795
Eco147I AGGCCT 2 cut(s) 232, 2562
Eco24I GRGCYC 1 cut(s) 429
Eco31I GGTCTC 2 cut(s) 1626, 1706
Eco47I GGWCC 2 cut(s) 1037, 2276
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 5 cut(s) 378, 1155, 1667, 2472, 2750
Eco88I CYCGRG 3 cut(s) 765, 1847, 1928
Eco91I GGTNACC 1 cut(s) 2446
EcoICRI GAGCTC 1 cut(s) 427
EcoO109I RGGNCCY 1 cut(s) 2276
EcoO65I GGTNACC 1 cut(s) 2446
EcoRI GAATTC 1 cut(s) 180
EcoRII CCWGG 2 cut(s) 1039, 1597
EcoT14I CCWWGG 3 cut(s) 503, 730, 1795
EcoT22I ATGCAT 3 cut(s) 344, 1542, 2632
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 3 cut(s) 503, 730, 1795
FalI AAGNNNNNCTT 2 cut(s) 855, 887
FaqI GGGAC 2 cut(s) 1161, 2289
FauI CCCGC 3 cut(s) 140, 671, 732
FbaI TGATCA 1 cut(s) 6
FblI GTMKAC 2 cut(s) 620, 2578
Fnu4HI GCNGC 5 cut(s) 404, 513, 701, 930, 947
FokI GGATG 8 cut(s) 209, 329, 560, 926, 1385, 1776, 2130, 2715
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 5 cut(s) 404, 513, 701, 930, 947
FspBI CTAG 6 cut(s) 261, 879, 1451, 1466, 1860, 2582
GluI GCNGC 5 cut(s) 404, 513, 701, 930, 947
GsaI CCCAGC 2 cut(s) 547, 1567
HaeIII GGCC 6 cut(s) 232, 357, 849, 2022, 2430, 2562
HgaI GACGC 4 cut(s) 57, 209, 655, 1771
Hin1I GRCGYC 1 cut(s) 68
HincII GTYRAC 3 cut(s) 72, 637, 996
HindII GTYRAC 3 cut(s) 72, 637, 996
HindIII AAGCTT 2 cut(s) 1811, 2510
HphI GGTGA 5 cut(s) 16, 178, 2414, 2458, 2471
Hpy166II GTNNAC 8 cut(s) 19, 72, 621, 637, 996, 1580, 2479, 2579
Hpy188I TCNGA 8 cut(s) 40, 51, 174, 397, 480, 1737, 2263, 2379
Hpy8I GTNNAC 8 cut(s) 19, 72, 621, 637, 996, 1580, 2479, 2579
Hpy99I CGWCG 2 cut(s) 87, 671
HpyAV CCTTC 9 cut(s) 377, 402, 1153, 1417, 1754, 2399, 2492, 2496, 2573
HpyCH4III ACNGT 3 cut(s) 1533, 1584, 2165
HpyCH4IV ACGT 3 cut(s) 1344, 1606, 1951
HpyF10VI GCNNNNNNNGC 2 cut(s) 1546, 2509
HpyF3I CTNAG 8 cut(s) 110, 522, 1227, 1964, 2039, 2384, 2459, 2508
HpySE526I ACGT 3 cut(s) 1344, 1606, 1951
Hsp92I GRCGYC 1 cut(s) 68
Ksp22I TGATCA 1 cut(s) 6
LmnI GCTCC 3 cut(s) 460, 926, 2408
Lsp1109I GCAGC 3 cut(s) 499, 712, 916
LweI GCATC 8 cut(s) 538, 1090, 1207, 1527, 2108, 2617, 2639, 2693
MaeI CTAG 6 cut(s) 261, 879, 1451, 1466, 1860, 2582
MaeII ACGT 3 cut(s) 1344, 1606, 1951
MaeIII GTNAC 2 cut(s) 1438, 2446
MfeI CAATTG 3 cut(s) 249, 2004, 2674
MflI RGATCY 6 cut(s) 263, 388, 726, 1502, 1753, 2258
MhlI GDGCHC 1 cut(s) 429
MlsI TGGCCA 1 cut(s) 2430
MluNI TGGCCA 1 cut(s) 2430
MlyI GAGTC 2 cut(s) 44, 1861
MmeI TCCRAC 3 cut(s) 1545, 1918, 1926
Mox20I TGGCCA 1 cut(s) 2430
Mph1103I ATGCAT 3 cut(s) 344, 1542, 2632
MroXI GAANNNNTTC 5 cut(s) 658, 870, 1140, 1572, 2735
MscI TGGCCA 1 cut(s) 2430
MslI CAYNNNNRTG 3 cut(s) 14, 191, 653
Msp20I TGGCCA 1 cut(s) 2430
MspR9I CCNGG 2 cut(s) 1041, 1599
MunI CAATTG 3 cut(s) 249, 2004, 2674
MvaI CCWGG 2 cut(s) 1041, 1599
MvnI CGCG 1 cut(s) 666
MwoI GCNNNNNNNGC 2 cut(s) 1546, 2509
NcoI CCATGG 1 cut(s) 730
NlaIV GGNNCC 6 cut(s) 390, 407, 441, 728, 2260, 2277
NmuCI GTSAC 2 cut(s) 1438, 2446
NsiI ATGCAT 3 cut(s) 344, 1542, 2632
NspI RCATGY 1 cut(s) 739
PaeI GCATGC 1 cut(s) 739
PaeR7I CTCGAG 2 cut(s) 765, 1847
PagI TCATGA 1 cut(s) 1624
PceI AGGCCT 2 cut(s) 232, 2562
PdmI GAANNNNTTC 5 cut(s) 658, 870, 1140, 1572, 2735
PflFI GACNNNGTC 1 cut(s) 1472
PfoI TCCNGGA 1 cut(s) 1039
PkrI GCNGC 5 cut(s) 405, 514, 702, 931, 948
PleI GAGTC 2 cut(s) 43, 1860
PpsI GAGTC 2 cut(s) 43, 1860
PpuMI RGGWCCY 1 cut(s) 2276
PshAI GACNNNNGTC 1 cut(s) 524
PshBI ATTAAT 1 cut(s) 1892
Psp124BI GAGCTC 1 cut(s) 429
Psp1406I AACGTT 1 cut(s) 1951
Psp5II RGGWCCY 1 cut(s) 2276
Psp6I CCWGG 2 cut(s) 1039, 1597
PspEI GGTNACC 1 cut(s) 2446
PspFI CCCAGC 2 cut(s) 543, 1563
PspGI CCWGG 2 cut(s) 1039, 1597
PspN4I GGNNCC 6 cut(s) 390, 407, 441, 728, 2260, 2277
PspPI GGNCC 3 cut(s) 355, 1037, 2276
PspPPI RGGWCCY 1 cut(s) 2276
PspXI VCTCGAGB 1 cut(s) 765
PstI CTGCAG 2 cut(s) 702, 2051
PstNI CAGNNNCTG 1 cut(s) 929
PsuI RGATCY 6 cut(s) 263, 388, 726, 1502, 1753, 2258
PsyI GACNNNGTC 1 cut(s) 1472
RsaI GTAC 3 cut(s) 1491, 2605, 2751
RsaNI GTAC 3 cut(s) 1490, 2604, 2750
RseI CAYNNNNRTG 3 cut(s) 14, 191, 653
SacI GAGCTC 1 cut(s) 429
SatI GCNGC 5 cut(s) 404, 513, 701, 930, 947
Sau96I GGNCC 3 cut(s) 355, 1037, 2276
SchI GAGTC 2 cut(s) 44, 1861
ScrFI CCNGG 2 cut(s) 1041, 1599
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 8 cut(s) 538, 1090, 1207, 1527, 2108, 2617, 2639, 2693
SfcI CTRYAG 2 cut(s) 698, 2047
Sfr274I CTCGAG 2 cut(s) 765, 1847
SinI GGWCC 2 cut(s) 1037, 2276
SlaI CTCGAG 2 cut(s) 765, 1847
SmiMI CAYNNNNRTG 3 cut(s) 14, 191, 653
SmlI CTYRAG 5 cut(s) 209, 765, 1742, 1847, 2513
SmoI CTYRAG 5 cut(s) 209, 765, 1742, 1847, 2513
SphI GCATGC 1 cut(s) 739
SseBI AGGCCT 2 cut(s) 232, 2562
SsiI CCGC 6 cut(s) 133, 403, 664, 739, 946, 1244
SspI AATATT 1 cut(s) 1392
SspMI CTAG 6 cut(s) 261, 879, 1451, 1466, 1860, 2582
SstI GAGCTC 1 cut(s) 429
StuI AGGCCT 2 cut(s) 232, 2562
StyD4I CCNGG 2 cut(s) 1039, 1597
StyI CCWWGG 3 cut(s) 503, 730, 1795
TaaI ACNGT 3 cut(s) 1533, 1584, 2165
TaiI ACGT 3 cut(s) 1347, 1609, 1954
TaqI TCGA 5 cut(s) 62, 669, 766, 1535, 1848
TaqII GACCGA 1 cut(s) 816
TatI WGTACW 2 cut(s) 1489, 2603
TauI GCSGC 2 cut(s) 406, 949
TscAI CASTG 2 cut(s) 1288, 2616
TseFI GTSAC 2 cut(s) 1438, 2446
TseI GCWGC 3 cut(s) 512, 700, 929
Tsp45I GTSAC 2 cut(s) 1438, 2446
TspGWI ACGGA 2 cut(s) 4, 604
TspRI CASTG 2 cut(s) 1288, 2616
Tth111I GACNNNGTC 1 cut(s) 1472
VpaK11BI GGWCC 2 cut(s) 1037, 2276
VspI ATTAAT 1 cut(s) 1892
XapI RAATTY 6 cut(s) 180, 783, 1660, 1771, 2423, 2635
XbaI TCTAGA 2 cut(s) 260, 878
XceI RCATGY 1 cut(s) 739
XcmI CCANNNNNNNNNTGG 1 cut(s) 1793
XhoI CTCGAG 2 cut(s) 765, 1847
XmiI GTMKAC 2 cut(s) 620, 2578
XmnI GAANNNNTTC 5 cut(s) 658, 870, 1140, 1572, 2735
XspI CTAG 6 cut(s) 261, 879, 1451, 1466, 1860, 2582
Zsp2I ATGCAT 3 cut(s) 344, 1542, 2632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.