Rroxscaffold_2G00121250

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000105
Physical Location & Seq
Reverse (-)
53758327 .. 53763100
4774 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00121250.1

Sequence Viewer

Length: 2631 bp
ATGCTTAACAATATTGTAAATGTATCTGACAAGGATATGGGAAGAGATATGATCAGGCAAAGACTACGTACTACAATGGTTCTTATCGTTCTTGATGATGTGGATAAACTAGAACAATTGGAAGCATTGTGTGATTGTAGTTGGTTTGGGCCAGGGAGTCGAATCATCATAACATCAAGAGATCAGCATTTGCTGAGACAATTTGGAGTAGATAGCATGTACAAGGTTGAAAGATTAACTGAGGCTGAAGCTCTTGAGCTGTTTAGTCAGATGGCCTTCAAGAAAGATCAAGCAGGAGAAGATCTTCAGAAGCTATCTAAGGAATTTGTGAAATTTGCTGCTGGCCTTCCATTAGTTCTCAAAGTTTTGGGTGCCTATCTATATGGTAGAAGTATACAACAATGGTTAGCTTCCTTGGATAGACTAAAACAACAACCTGATCGAGTAATTGCTCAGATGCTTAGAACTGGTAGAAGTTATGATGAACTAGAAGAATCAACATCAATTGCTGATGTGCTTAGATTAAGTTATGATGGACTAGAAGAATCAACACAAGTTGCTTTTTTGGACATTGCATGTTTCTTTAAAGGGGAGAATATAGATCGTGTAAAAAGAATACTACATGGATGGGACGGATGGGACAACTGGATTTCATTCTGGGATATAAAAGTTCTAGCGGAGAAAGCTCTAGTAACTGTGGATGGAAGAAAATTATGGATGCATGACCTGATACAACAATTGGGCCAAAAAATTGTTTCTCAAGAATGTATTGAGCCTGGGAAGCGTAGTAGGTTGTGGATCCCGAAAGATATCATCCATGTACTTGAAAAGAATACGGGTTCAAGTTCACTTGAAAGCATAGCCTTAGACTTTCCAAAAACAGAAGAGGTACATTGCAGCTCAGAGGCATTCTCTAAGATGGACAGACTTCGGATTCTCCAAATCCGGAATGTGCATATGAAGGAAGGCCCTAGATATCTTTCTCAAGAGTTAATGCTTCTGGAATGGAGTGGTTATCCTACAGAAAGTCTCCCACAAAATTTCAATCCTGATAGACTATGTGAACTTAACCTCTGCTATAGCATGATCAAACGACTTTGGACCGGAAGAAAGGACATGCCCTGCTTGACAGACATCAATTTAAGCTACTCTTGGAAGTTAACCAGGACCCCAGATTTTACATGTGCTCCACGGCTTGAAAGATTAATTCTTGTAGATTGTACAAAATTGGTGGAGGTCCACCCATCCATTGGTTTTCTTAAAATGCTCACTTGCTTGAATCTTAAAGATTGCACAAGTCTTGAAAGTCTTCCAGGTAAGCTTGAAACGAAATGCCTTAGAATTCTTGTTCTTTCAGGCTGTTCAAAAGTGAAGAAAATTCCAGATTTTGTGGGATCTATGGAATGCTTGTTAGAACTTTATTTGGATGAGACTGCTATAGAGCAATTACCTATATCAGTTCGACTTCTGACTGGCCTCATCCTATTAAATTTGAGAGATTGCAAAAATCTTAAGAGTCTTCCAAGCGATATTGGCAATTTGATGTCCCTCAAGCTTCTCAATATCTGTGGATGCTCAAAACTTGAAGCACTACCAGACAGCTTGGGAAAGATAGACTGCTTGGAAGAGCTTGATGCGGGCAGTACCTCCGTTAGCGCCTTACCAACGTCCATTTCTCTTCTGGGAAATCTTAAAGTTTTATCTCTTCATGGATGTAAAGGGATGCCAGGCAAGAGGCCAAGATCTCCGCGCAGTTTTCGATTGCCCATTACTGATTCAGGTGGTTTGCGTTCTTTAACCGAACTGAATCTTAGTGACTGTAATCTTCAGGAGGGAACAATTCCAAAAGATATTGGCTGCTTATCGTCATTGGTATCACTAAATGTAAGCAAAAACTGTTTTGTTAGTCTTCCCAAAAGCATTAGACAGCTTTCAAAGCTTCAGAAGTTGAATTTGGAAAGTTGCAAGAGTCTTCAAAATTTGCCGGAACTTTCATCAAGAATAACCTGTAGTGCTGGGGCAGAAGGTGGTAATTTAGAGAAAAGACTGTCATCATGTTTCAATTTGCTTAATTGCTTCCAATTGGTTGTCAATGAGAAATGCACAAATATAGCGCTTGCAATGCTATGGAGATCTCTTCAGGGAATTTCTTATTCAGGAAATAGTTTTGAAACTATAATTCCTGGCAGTGAAATGTCTGAGCTGTTCAGTGATTGGAGTATGGGACCAGTAGTAAGCATGGAACTACCTCGACAATGGCACAAGAATAAGTGGATGGGATATGCATTTTGTGCTTGTTTTGTTATCCGTCGAGATCTTCCAGTTCCAGCCAACCGGCTTGGTAAATGGAAGTTCGGGACCCATAGCACTGCACATGGTCTTCGATGCGAAGTGAAGTCACTGGCTGACTGGTGTCCTTCCTTTGCCTGCAGCCAAGAATTAGGCCAAATTGAGTCGGAACACCTTTGGCTATCCTTTGTCTCTGGTGATTATTTTGGAACAGCATGGCAAGAAACCTGCCTTAACATTGAGTTTACATTTAAAACATTAGGCACTGGTATGGAGGTGAAGAAGTGTGGAGTCCGTTTGATATACGAGCACGATTTAGATTTGATTGGAATGAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

876

Amino Acids

98.86

Weight (kDa)

7.35

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 14 - 96 1.6e-09 NB-ARC domain
WHD_ROQ1 PF23282 182 - 253 2e-10 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 412 - 523 2e-08 Leucine-rich repeat region
LRR_13 PF23286 445 - 529 7.9e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 495 - 576 1.9e-09 Leucine-rich repeat region
LRR_14 PF23598 596 - 661 5.5e-06 Leucine-rich repeat region
C-JID PF20160 728 - 869 6.9e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2525
AccB1I GGYRCC 1 cut(s) 371
AccB7I CCANNNNNTGG 1 cut(s) 1250
AccI GTMKAC 1 cut(s) 394
AccII CGCG 1 cut(s) 1750
AccIII TCCGGA 1 cut(s) 945
AciI CCGC 3 cut(s) 677, 1637, 1748
AclWI GGATC 3 cut(s) 793, 806, 1402
AcsI RAATTY 9 cut(s) 323, 332, 1039, 1341, 1377, 1489, 1951, 1978, 2145
AcuI CTGAAG 5 cut(s) 267, 290, 1811, 1925, 2123
AfaI GTAC 6 cut(s) 70, 221, 822, 891, 1222, 1645
AfeI AGCGCT 1 cut(s) 2115
AfiI CCNNNNNNNGG 1 cut(s) 1250
AflII CTTAAG 1 cut(s) 1511
AflIII ACRYGT 1 cut(s) 1181
AjnI CCWGG 6 cut(s) 151, 775, 1163, 1312, 1726, 2182
AjuI GAANNNNNNNTTGG 4 cut(s) 1657, 1689, 1937, 1969
Alw21I GWGCWC 2 cut(s) 1189, 2601
Alw26I GTCTC 4 cut(s) 190, 1034, 1424, 2485
AlwI GGATC 3 cut(s) 793, 806, 1402
Aor13HI TCCGGA 1 cut(s) 945
Aor51HI AGCGCT 1 cut(s) 2115
AoxI GGCC 8 cut(s) 149, 273, 343, 742, 967, 1474, 1736, 2443
ApeKI GCWGC 4 cut(s) 338, 897, 1857, 2430
ApoI RAATTY 9 cut(s) 323, 332, 1039, 1341, 1377, 1489, 1951, 1978, 2145
AseI ATTAAT 1 cut(s) 1205
Asp700I GAANNNNTTC 2 cut(s) 303, 1308
AspLEI GCGC 3 cut(s) 1658, 1752, 2116
AspS9I GGNCC 8 cut(s) 149, 742, 968, 1101, 1167, 1237, 2225, 2358
AsuHPI GGTGA 2 cut(s) 2498, 2578
AvaII GGWCC 5 cut(s) 1101, 1167, 1237, 2225, 2358
BamHI GGATCC 1 cut(s) 798
BanI GGYRCC 1 cut(s) 371
BbsI GAAGAC 5 cut(s) 1301, 1511, 1901, 1964, 2372
Bbv12I GWGCWC 2 cut(s) 1189, 2601
BbvI GCAGC 4 cut(s) 325, 909, 1844, 2442
BccI CCATC 8 cut(s) 265, 527, 621, 630, 695, 913, 1252, 2269
BceAI ACGGC 1 cut(s) 1208
BciT130I CCWGG 6 cut(s) 153, 777, 1165, 1314, 1728, 2184
BclI TGATCA 2 cut(s) 51, 1086
BcoDI GTCTC 4 cut(s) 190, 1034, 1424, 2485
BfaI CTAG 6 cut(s) 110, 488, 539, 674, 689, 972
BfmI CTRYAG 5 cut(s) 1020, 1078, 1437, 2008, 2428
BfoI RGCGCY 2 cut(s) 1659, 2117
BfrI CTTAAG 1 cut(s) 1511
BfuAI ACCTGC 1 cut(s) 2525
BglII AGATCT 4 cut(s) 301, 1742, 2132, 2314
BisI GCNGC 4 cut(s) 339, 898, 1858, 2431
BlsI GCNGC 4 cut(s) 340, 899, 1859, 2432
Bme1390I CCNGG 6 cut(s) 153, 777, 1165, 1314, 1728, 2184
Bme18I GGWCC 5 cut(s) 1101, 1167, 1237, 2225, 2358
BmgT120I GGNCC 8 cut(s) 149, 742, 968, 1101, 1167, 1237, 2225, 2358
BmiI GGNNCC 6 cut(s) 373, 800, 1169, 2226, 2359, 2360
BmrFI CCNGG 6 cut(s) 153, 777, 1165, 1314, 1728, 2184
BmsI GCATC 6 cut(s) 447, 708, 1562, 1624, 1713, 2375
BoxI GACNNNNGTC 1 cut(s) 2412
BpiI GAAGAC 5 cut(s) 1301, 1511, 1901, 1964, 2372
BplI GAGNNNNNCTC 2 cut(s) 896, 928
BpuEI CTTGAG 4 cut(s) 275, 744, 969, 1535
BsaAI YACGTR 1 cut(s) 68
BsaJI CCNNGG 4 cut(s) 152, 414, 776, 1190
BsaWI WCCGGW 2 cut(s) 945, 1103
BsaXI ACNNNNNCTCC 2 cut(s) 1171, 1201
Bsc4I CCNNNNNNNGG 1 cut(s) 1250
Bse118I RCCGGY 1 cut(s) 2334
Bse1I ACTGG 8 cut(s) 472, 650, 1477, 2228, 2321, 2406, 2414, 2560
Bse3DI GCAATG 3 cut(s) 570, 892, 2127
BseAI TCCGGA 1 cut(s) 945
BseBI CCWGG 6 cut(s) 153, 777, 1165, 1314, 1728, 2184
BseDI CCNNGG 4 cut(s) 152, 414, 776, 1190
BseLI CCNNNNNNNGG 1 cut(s) 1250
BseMI GCAATG 3 cut(s) 570, 892, 2127
BseMII CTCAG 5 cut(s) 185, 231, 467, 915, 2190
BseNI ACTGG 8 cut(s) 472, 650, 1477, 2228, 2321, 2406, 2414, 2560
BseXI GCAGC 4 cut(s) 325, 909, 1844, 2442
BseYI CCCAGC 1 cut(s) 2015
BsgI GTGCAG 1 cut(s) 2355
Bsh1236I CGCG 1 cut(s) 1750
BshFI GGCC 8 cut(s) 151, 275, 345, 744, 969, 1476, 1738, 2445
BshNI GGYRCC 1 cut(s) 371
BsiHKAI GWGCWC 2 cut(s) 1189, 2601
BsiSI CCGG 4 cut(s) 946, 1104, 1985, 2335
BslFI GGGAC 5 cut(s) 644, 653, 1531, 2238, 2371
BslI CCNNNNNNNGG 1 cut(s) 1250
BsmAI GTCTC 4 cut(s) 190, 1034, 1424, 2485
BsmFI GGGAC 5 cut(s) 644, 653, 1531, 2238, 2371
BsmI GAATGC 2 cut(s) 908, 1409
BsnI GGCC 8 cut(s) 151, 275, 345, 744, 969, 1476, 1738, 2445
Bsp1286I GDGCHC 2 cut(s) 1189, 2601
Bsp13I TCCGGA 1 cut(s) 945
Bsp1407I TGTACA 2 cut(s) 219, 1220
BspACI CCGC 3 cut(s) 677, 1637, 1748
BspANI GGCC 8 cut(s) 151, 275, 345, 744, 969, 1476, 1738, 2445
BspCNI CTCAG 5 cut(s) 186, 232, 466, 914, 2191
BspEI TCCGGA 1 cut(s) 945
BspFNI CGCG 1 cut(s) 1750
BspLI GGNNCC 6 cut(s) 373, 800, 1169, 2226, 2359, 2360
BspMAI CTGCAG 1 cut(s) 2432
BspMI ACCTGC 1 cut(s) 2525
BspPI GGATC 3 cut(s) 793, 806, 1402
BspQI GCTCTTC 1 cut(s) 1620
BspT107I GGYRCC 1 cut(s) 371
BspTI CTTAAG 1 cut(s) 1511
BsrDI GCAATG 3 cut(s) 570, 892, 2127
BsrFI RCCGGY 1 cut(s) 2334
BsrGI TGTACA 2 cut(s) 219, 1220
BsrI ACTGG 8 cut(s) 472, 650, 1477, 2228, 2321, 2406, 2414, 2560
BssAI RCCGGY 1 cut(s) 2334
BssECI CCNNGG 4 cut(s) 152, 414, 776, 1190
BssNAI GTATAC 1 cut(s) 395
BssT1I CCWWGG 1 cut(s) 414
Bst1107I GTATAC 1 cut(s) 395
Bst2UI CCWGG 6 cut(s) 153, 777, 1165, 1314, 1728, 2184
Bst4CI ACNGT 4 cut(s) 697, 1820, 1898, 2049
Bst6I CTCTTC 6 cut(s) 37, 879, 1620, 1683, 1710, 2142
BstAFI CTTAAG 1 cut(s) 1511
BstAPI GCANNNNNTGC 1 cut(s) 2291
BstAUI TGTACA 2 cut(s) 219, 1220
BstBAI YACGTR 1 cut(s) 68
BstC8I GCNNGC 4 cut(s) 343, 1639, 2118, 2428
BstDSI CCRYGG 1 cut(s) 1190
BstFNI CGCG 1 cut(s) 1750
BstH2I RGCGCY 2 cut(s) 1659, 2117
BstHHI GCGC 3 cut(s) 1658, 1752, 2116
BstMAI GTCTC 4 cut(s) 190, 1034, 1424, 2485
BstMWI GCNNNNNNNGC 6 cut(s) 683, 781, 1533, 1936, 2122, 2291
BstNI CCWGG 6 cut(s) 153, 777, 1165, 1314, 1728, 2184
BstNSI RCATGY 4 cut(s) 220, 579, 1120, 1185
BstPAI GACNNNNGTC 1 cut(s) 2412
BstSCI CCNGG 6 cut(s) 151, 775, 1163, 1312, 1726, 2182
BstSFI CTRYAG 5 cut(s) 1020, 1078, 1437, 2008, 2428
BstSNI TACGTA 1 cut(s) 68
BstUI CGCG 1 cut(s) 1750
BstV1I GCAGC 4 cut(s) 325, 909, 1844, 2442
BstV2I GAAGAC 5 cut(s) 1301, 1511, 1901, 1964, 2372
BstX2I RGATCY 6 cut(s) 301, 798, 1394, 1742, 2132, 2314
BstYI RGATCY 6 cut(s) 301, 798, 1394, 1742, 2132, 2314
BstZ17I GTATAC 1 cut(s) 395
BsuRI GGCC 8 cut(s) 151, 275, 345, 744, 969, 1476, 1738, 2445
BtgI CCRYGG 1 cut(s) 1190
BtsI GCAGTG 2 cut(s) 2194, 2367
BtsIMutI CAGTG 5 cut(s) 2194, 2215, 2367, 2399, 2553
BveI ACCTGC 1 cut(s) 2525
Cac8I GCNNGC 4 cut(s) 343, 1639, 2118, 2428
CfoI GCGC 3 cut(s) 1658, 1752, 2116
Cfr10I RCCGGY 1 cut(s) 2334
Cfr13I GGNCC 8 cut(s) 149, 742, 968, 1101, 1167, 1237, 2225, 2358
Csp6I GTAC 6 cut(s) 69, 220, 821, 890, 1221, 1644
CspCI CAANNNNNGTGG 4 cut(s) 1212, 1247, 1549, 1584
CviQI GTAC 6 cut(s) 69, 220, 821, 890, 1221, 1644
DraI TTTAAA 2 cut(s) 586, 2542
Eam1104I CTCTTC 6 cut(s) 37, 879, 1620, 1683, 1710, 2142
EarI CTCTTC 6 cut(s) 37, 879, 1620, 1683, 1710, 2142
Eco105I TACGTA 1 cut(s) 68
Eco130I CCWWGG 1 cut(s) 414
Eco32I GATATC 2 cut(s) 811, 977
Eco47I GGWCC 5 cut(s) 1101, 1167, 1237, 2225, 2358
Eco47III AGCGCT 1 cut(s) 2115
Eco57I CTGAAG 5 cut(s) 267, 290, 1811, 1925, 2123
EcoO109I RGGNCCY 3 cut(s) 968, 1167, 2358
EcoRI GAATTC 1 cut(s) 1341
EcoRII CCWGG 6 cut(s) 151, 775, 1163, 1312, 1726, 2182
EcoRV GATATC 2 cut(s) 811, 977
EcoT14I CCWWGG 1 cut(s) 414
EcoT22I ATGCAT 2 cut(s) 723, 2287
ErhI CCWWGG 1 cut(s) 414
FalI AAGNNNNNCTT 2 cut(s) 1135, 1167
FaqI GGGAC 5 cut(s) 644, 653, 1531, 2238, 2371
FauI CCCGC 1 cut(s) 1630
FauNDI CATATG 1 cut(s) 957
FbaI TGATCA 2 cut(s) 51, 1086
FblI GTMKAC 1 cut(s) 394
Fnu4HI GCNGC 4 cut(s) 339, 898, 1858, 2431
Fsp4HI GCNGC 4 cut(s) 339, 898, 1858, 2431
FspBI CTAG 6 cut(s) 110, 488, 539, 674, 689, 972
GlaI GCGC 3 cut(s) 1657, 1751, 2115
GluI GCNGC 4 cut(s) 339, 898, 1858, 2431
GsaI CCCAGC 1 cut(s) 2019
HaeII RGCGCY 2 cut(s) 1659, 2117
HaeIII GGCC 8 cut(s) 151, 275, 345, 744, 969, 1476, 1738, 2445
HapII CCGG 4 cut(s) 946, 1104, 1985, 2335
HhaI GCGC 3 cut(s) 1658, 1752, 2116
Hin6I GCGC 3 cut(s) 1656, 1750, 2114
HinP1I GCGC 3 cut(s) 1656, 1750, 2114
HincII GTYRAC 1 cut(s) 1161
HindII GTYRAC 1 cut(s) 1161
HindIII AAGCTT 3 cut(s) 1319, 1553, 1937
HpaI GTTAAC 1 cut(s) 1161
HpaII CCGG 4 cut(s) 946, 1104, 1985, 2335
HphI GGTGA 2 cut(s) 2498, 2578
Hpy166II GTNNAC 6 cut(s) 395, 848, 1064, 1161, 1240, 2535
Hpy8I GTNNAC 6 cut(s) 395, 848, 1064, 1161, 1240, 2535
Hpy99I CGWCG 1 cut(s) 2313
HpyAV CCTTC 6 cut(s) 286, 356, 955, 959, 2018, 2427
HpyCH4III ACNGT 4 cut(s) 697, 1820, 1898, 2049
HpyCH4IV ACGT 2 cut(s) 67, 1667
HpyF10VI GCNNNNNNNGC 6 cut(s) 683, 781, 1533, 1936, 2122, 2291
HpySE526I ACGT 2 cut(s) 67, 1667
HspAI GCGC 3 cut(s) 1656, 1750, 2114
KflI GGGWCCC 1 cut(s) 2358
Kpn2I TCCGGA 1 cut(s) 945
Ksp22I TGATCA 2 cut(s) 51, 1086
KspAI GTTAAC 1 cut(s) 1161
LguI GCTCTTC 1 cut(s) 1620
LmnI GCTCC 1 cut(s) 1192
Lsp1109I GCAGC 4 cut(s) 325, 909, 1844, 2442
LweI GCATC 6 cut(s) 447, 708, 1562, 1624, 1713, 2375
MaeI CTAG 6 cut(s) 110, 488, 539, 674, 689, 972
MaeII ACGT 2 cut(s) 67, 1667
MaeIII GTNAC 3 cut(s) 691, 1814, 2397
MfeI CAATTG 4 cut(s) 116, 504, 737, 2081
MflI RGATCY 6 cut(s) 301, 798, 1394, 1742, 2132, 2314
MhlI GDGCHC 2 cut(s) 1189, 2601
MlyI GAGTC 5 cut(s) 166, 1525, 1978, 2462, 2589
MmeI TCCRAC 1 cut(s) 2436
Mph1103I ATGCAT 2 cut(s) 723, 2287
MroI TCCGGA 1 cut(s) 945
MroXI GAANNNNTTC 2 cut(s) 303, 1308
MslI CAYNNNNRTG 1 cut(s) 2558
MspCI CTTAAG 1 cut(s) 1511
MspI CCGG 4 cut(s) 946, 1104, 1985, 2335
MspR9I CCNGG 6 cut(s) 153, 777, 1165, 1314, 1728, 2184
MunI CAATTG 4 cut(s) 116, 504, 737, 2081
Mva1269I GAATGC 2 cut(s) 908, 1409
MvaI CCWGG 6 cut(s) 153, 777, 1165, 1314, 1728, 2184
MvnI CGCG 1 cut(s) 1750
MwoI GCNNNNNNNGC 6 cut(s) 683, 781, 1533, 1936, 2122, 2291
NdeI CATATG 1 cut(s) 957
NlaIV GGNNCC 6 cut(s) 373, 800, 1169, 2226, 2359, 2360
NmuCI GTSAC 2 cut(s) 1814, 2397
NsiI ATGCAT 2 cut(s) 723, 2287
NspI RCATGY 4 cut(s) 220, 579, 1120, 1185
PciI ACATGT 1 cut(s) 1181
PciSI GCTCTTC 1 cut(s) 1620
PctI GAATGC 2 cut(s) 908, 1409
PdmI GAANNNNTTC 2 cut(s) 303, 1308
PfeI GAWTC 7 cut(s) 162, 494, 545, 934, 1279, 1775, 1807
PflMI CCANNNNNTGG 1 cut(s) 1250
PkrI GCNGC 4 cut(s) 340, 899, 1859, 2432
PleI GAGTC 5 cut(s) 165, 1524, 1977, 2461, 2588
PpsI GAGTC 5 cut(s) 165, 1524, 1977, 2461, 2588
Ppu21I YACGTR 1 cut(s) 68
PpuMI RGGWCCY 2 cut(s) 1167, 2358
PscI ACATGT 1 cut(s) 1181
PshAI GACNNNNGTC 1 cut(s) 2412
PshBI ATTAAT 1 cut(s) 1205
Psp5II RGGWCCY 2 cut(s) 1167, 2358
Psp6I CCWGG 6 cut(s) 151, 775, 1163, 1312, 1726, 2182
PspFI CCCAGC 1 cut(s) 2015
PspGI CCWGG 6 cut(s) 151, 775, 1163, 1312, 1726, 2182
PspN4I GGNNCC 6 cut(s) 373, 800, 1169, 2226, 2359, 2360
PspPI GGNCC 8 cut(s) 149, 742, 968, 1101, 1167, 1237, 2225, 2358
PspPPI RGGWCCY 2 cut(s) 1167, 2358
PstI CTGCAG 1 cut(s) 2432
PsuI RGATCY 6 cut(s) 301, 798, 1394, 1742, 2132, 2314
RsaI GTAC 6 cut(s) 70, 221, 822, 891, 1222, 1645
RsaNI GTAC 6 cut(s) 69, 220, 821, 890, 1221, 1644
RseI CAYNNNNRTG 1 cut(s) 2558
SapI GCTCTTC 1 cut(s) 1620
SatI GCNGC 4 cut(s) 339, 898, 1858, 2431
Sau96I GGNCC 8 cut(s) 149, 742, 968, 1101, 1167, 1237, 2225, 2358
SchI GAGTC 5 cut(s) 166, 1525, 1978, 2462, 2589
ScrFI CCNGG 6 cut(s) 153, 777, 1165, 1314, 1728, 2184
SduI GDGCHC 2 cut(s) 1189, 2601
SfaNI GCATC 6 cut(s) 447, 708, 1562, 1624, 1713, 2375
SfcI CTRYAG 5 cut(s) 1020, 1078, 1437, 2008, 2428
SinI GGWCC 5 cut(s) 1101, 1167, 1237, 2225, 2358
SmiMI CAYNNNNRTG 1 cut(s) 2558
SmlI CTYRAG 5 cut(s) 254, 759, 984, 1511, 1550
SmoI CTYRAG 5 cut(s) 254, 759, 984, 1511, 1550
SnaBI TACGTA 1 cut(s) 68
SsiI CCGC 3 cut(s) 677, 1637, 1748
SspI AATATT 1 cut(s) 13
SspMI CTAG 6 cut(s) 110, 488, 539, 674, 689, 972
StyD4I CCNGG 6 cut(s) 151, 775, 1163, 1312, 1726, 2182
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 4 cut(s) 697, 1820, 1898, 2049
TaiI ACGT 2 cut(s) 70, 1670
TaqI TCGA 7 cut(s) 160, 442, 1462, 1759, 2251, 2311, 2383
TatI WGTACW 3 cut(s) 219, 820, 1220
TfiI GAWTC 7 cut(s) 162, 494, 545, 934, 1279, 1775, 1807
TscAI CASTG 5 cut(s) 2194, 2215, 2374, 2406, 2560
TseFI GTSAC 2 cut(s) 1814, 2397
TseI GCWGC 4 cut(s) 338, 897, 1857, 2430
Tsp45I GTSAC 2 cut(s) 1814, 2397
TspDTI ATGAA 5 cut(s) 498, 642, 974, 1697, 1983
TspGWI ACGGA 4 cut(s) 648, 1639, 2297, 2573
TspRI CASTG 5 cut(s) 2194, 2215, 2374, 2406, 2560
Van91I CCANNNNNTGG 1 cut(s) 1250
Vha464I CTTAAG 1 cut(s) 1511
VpaK11BI GGWCC 5 cut(s) 1101, 1167, 1237, 2225, 2358
VspI ATTAAT 1 cut(s) 1205
XapI RAATTY 9 cut(s) 323, 332, 1039, 1341, 1377, 1489, 1951, 1978, 2145
XceI RCATGY 4 cut(s) 220, 579, 1120, 1185
XcmI CCANNNNNNNNNTGG 2 cut(s) 1247, 1678
XmiI GTMKAC 1 cut(s) 394
XmnI GAANNNNTTC 2 cut(s) 303, 1308
XspI CTAG 6 cut(s) 110, 488, 539, 674, 689, 972
Zsp2I ATGCAT 2 cut(s) 723, 2287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.