Rroxscaffold_2G00141590

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000105
Physical Location & Seq
Reverse (-)
79822161 .. 79827694
5534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141590.1

Sequence Viewer

Length: 2712 bp
ATGATTTTGAAAGTAGCTGCTTTCTTTCCCAATGTTAGAGAAAGGTCCAAAAAGGATGGTCTAGTTTCTTTACAAGAAGAACTTCTTTCCAGAATGCTGTCTACAGAAATTGGAATAGAGGATGCATATGCAGGAACTGCACTGATAAAAACACGGTTATGTAGGCGAAGAGTACTTGTTGTCATTGATGACGTTGATGATCTAACACAATTGGAAAAGTTGGCTGGATGTCATGACTGGTTCGGCCCGAGGAGTAGAGTGATCATAACCACCACAGATGTTCATTTGTTGAAGGCATATGGTGACGGTGTTGATAGTATATATGAGGCAACTGGATTACTATATGGTGAATCTCTTCAACTCTTATGTTTGAAGGCTTTTAAGAAGAGCCAACCGCCTGAAGATTATTTGCACCTGTGTCCCGATATTTTAAATTATGCACGCGGGCTGCCTTTAGCTCTTGAGGTCATTGGTTCCTTTCTATGTGGTAGAAACATGGGAGAATGGGAAAGTGCCATCGATAGGCTAAAGAATAAACCGGAAAGAAGTATTATCGATGTGCTACAGATAAGTTTCGATGGACTAGGGGAGACAGAAAAGGAAATATTTCTACATGTTGCATGCTTTTATAAGGGGAAGCCCAGGGATCGTGTAATACAAATACTAGACTATTGTCAGCTTTCCCCTGTCATTGGTTTAAGTGTTCTTGTTGACAAATCTCTCATTACTATTTCCAACAACAAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATAGTTCGCCGAGAGTCTCCTAAGAATCCAGGCAAACGTAGTAGATTATGGTCTTATGAAGACATCCACACTGTGCTGAAGAAAGATAAGGGAACAGAAGCAGTTCAAGGCATGGTGATGGACTTGCCTAAATTAGAAGCGGCTCAGTGGAAACCGGAAGCCTTCTCAAATTTGTCTCAACTTAGTTTCCTCCAAATTCGAAATGTGGACCTTCCCAAAGGCCTCACTTGTCTCTCTAATTCGTTGAAACTCCTCGAATGGAGTGGATACCCTGTAAGGTTTCTCCCACAAGATTTTGAATCAGATGAACTCATTGAACTTAACTTGTGCCACAGCAACATTGAACAGCTTTGGATAGGAGCAAAGAACTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAGCCTTTGTGAGACCCCAGACTTCACAGGCGTTCAAAATCTTGAACGATTAGATCTTGAAGGATGTAAAAAGTTGGTTAGAATTCATCCATCCCTTGGAGTTCTCAGAAAGCTTATTCTATTGAATCTTAAAGACTGTGAAAGTCTCAAGAGTCTGCCAGATAAAATCGAAATGGAATCTCTCGAAACTCTAATTCTTTCAAACTGCTCAAGTCTTAAGAAGATCCCCGAGTTTGTTGGATATATGGAACGTTTGTTGGTGCTTAATCTTGACAAGACTGCCATTGAAACACTGCCTCCCTCAATTGAGCGCTTAAGTGGTCTTGTTTCCTTGGATTTAAGCAACTGCAAAAATCTTGTTTCTCTTCCTCACACCATTAATGAATTGAAGTCCCTTCAAAGTCTTAGTGTTTCTGGATGCTTGAAACTTGGCGAACATCAGGGTAGTATGAGGGAGATGGAGTATATGGAGGGAAATGGTGCAAGTAGGACTGCTGTAGCAGAATTGCCATCATGGAAAGATAGTTTAAGCCATTGTTTTGAAGTGGTTCGGGAGTATTTCTCATGGTCCTTGCCTTCTAGATCAATGCAAAGAATCAATACAGAACCAATGAGTATGCAGTTGCATTTATCTGGACTCTGTAATTTAACATATCTAAACATAAGCAATTGCAATCTCGGTGATGGAGCATTTCGCAGCAACTTTGGCTGTTTTCCCTCATTGGTGGCATTGAATCTGGGTGGAAATGATTTTGTTAAGCTTCCTTCAAGCATCAAATCGTTTCTCAAGCTTGAAAACCTTAACTTGGAGAATTGCAAGAGGCTTAAAGAGTTGTCAGAGCTTCCATCAAATAGTAAATTGGATGTAAGGGCAGATGGCTGTTCATCGCTGGCAATGCTGTTTGATGCATCCAATTTCAATAGATTGGAGAAATCATATTTCAATTTCATCAATTGCTTCAGACTAAATGACAATCAAGGCTGCAGTAACGTAGCATTTGAAATGCTGAAGACATTCATTCCTCAGGGTATCTCGAATGCAAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAAAATTCCTGAGTGGTTCGGTCATCGAAGTGTTGGGAGTTCATTAAGTGTAGAGTTACCTCCATTTTGGAGTAACAGTAAGTTTATGGGGTTTGCTTTTTGTGCTGTTTTTGTACTCGATGAGCACCATGGAGTTGATATACTTAGCCAGCATCAATTCAAGACTTTCAATGCTACACATCATCTTGTTTGCTGCATGACCCTAAATGGGTTCGAGTTAAAACAAGTATATGGCAAACAGCCTGCATTTCGCTTTAGTGAAAATTTTTGCCAGGTTAAGTCAGATCACCTTTGGGCATTCTATGTATCCTCCGATAAATACTTTGCTGCAGAATGGTGGGATAGCAGTTGCAGTCAGCTTGAGTTCTTATTTAAAACCCGAGGGCCAGGTCTGAAGGTGAAGGAGTGCGGGGTCCATTTGATATATGAGCAAGGTGTACAGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

903

Amino Acids

102.08

Weight (kDa)

6.28

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 17 - 130 3.2e-07 NB-ARC domain
WHD_ROQ1 PF23282 197 - 267 5.3e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 456 - 543 2.5e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 484 - 712 9.3e-12 Leucine-rich repeat region
C-JID PF20160 752 - 901 3.9e-20 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 630
AccB7I CCANNNNNTGG 1 cut(s) 1286
AccI GTMKAC 1 cut(s) 101
AccII CGCG 1 cut(s) 444
AciI CCGC 4 cut(s) 395, 444, 923, 2673
AclI AACGTT 1 cut(s) 1441
AclWI GGATC 2 cut(s) 654, 1408
AcsI RAATTY 5 cut(s) 952, 978, 1272, 2268, 2527
AcuI CTGAAG 5 cut(s) 420, 881, 2134, 2219, 2678
AdeI CACNNNGTG 1 cut(s) 856
AfaI GTAC 3 cut(s) 174, 2379, 2703
AfeI AGCGCT 1 cut(s) 1502
AfiI CCNNNNNNNGG 5 cut(s) 522, 692, 1286, 1996, 2472
AflII CTTAAG 2 cut(s) 1406, 1504
AflIII ACRYGT 1 cut(s) 613
AjnI CCWGG 5 cut(s) 641, 811, 2257, 2535, 2650
AluBI AGCT 9 cut(s) 17, 458, 679, 1132, 1303, 1951, 1981, 2032, 2623
AluI AGCT 9 cut(s) 17, 458, 679, 1132, 1303, 1951, 1981, 2032, 2623
Alw21I GWGCWC 1 cut(s) 2391
Alw26I GTCTC 6 cut(s) 584, 804, 963, 1019, 1196, 1340
AlwI GGATC 2 cut(s) 654, 1408
AlwNI CAGNNNCTG 1 cut(s) 137
Ama87I CYCGRG 3 cut(s) 247, 1418, 2643
Aor51HI AGCGCT 1 cut(s) 1502
AoxI GGCC 3 cut(s) 244, 1003, 2648
ApeKI GCWGC 6 cut(s) 17, 448, 1887, 2172, 2457, 2591
ApoI RAATTY 5 cut(s) 952, 978, 1272, 2268, 2527
AseI ATTAAT 1 cut(s) 1569
Asp700I GAANNNNTTC 8 cut(s) 81, 354, 606, 786, 885, 1737, 2204, 2241
AspLEI GCGC 1 cut(s) 1503
AspS9I GGNCC 6 cut(s) 45, 245, 991, 1758, 2648, 2677
AsuHPI GGTGA 6 cut(s) 314, 359, 910, 1883, 2543, 2674
AsuII TTCGAA 1 cut(s) 982
AvaI CYCGRG 3 cut(s) 247, 1418, 2643
AvaII GGWCC 4 cut(s) 45, 991, 1758, 2677
AxyI CCTNAGG 1 cut(s) 2214
BbsI GAAGAC 2 cut(s) 849, 2207
Bbv12I GWGCWC 1 cut(s) 2391
BbvI GCAGC 6 cut(s) 4, 435, 1899, 2159, 2444, 2578
BciT130I CCWGG 5 cut(s) 643, 813, 2259, 2537, 2652
BciVI GTATCC 2 cut(s) 1043, 2581
BclI TGATCA 1 cut(s) 261
BcoDI GTCTC 6 cut(s) 584, 804, 963, 1019, 1196, 1340
BfaI CTAG 4 cut(s) 62, 584, 665, 1770
BfmI CTRYAG 5 cut(s) 102, 563, 1686, 2173, 2592
BfoI RGCGCY 1 cut(s) 1504
BfrI CTTAAG 2 cut(s) 1406, 1504
BfuI GTATCC 2 cut(s) 1043, 2581
BglII AGATCT 1 cut(s) 1243
BisI GCNGC 7 cut(s) 18, 449, 924, 1888, 2173, 2458, 2592
BlsI GCNGC 7 cut(s) 19, 450, 925, 1889, 2174, 2459, 2593
BmcAI AGTACT 1 cut(s) 174
Bme1390I CCNGG 5 cut(s) 643, 813, 2259, 2537, 2652
Bme18I GGWCC 4 cut(s) 45, 991, 1758, 2677
BmeT110I CYCGRG 3 cut(s) 247, 1418, 2643
BmgT120I GGNCC 6 cut(s) 45, 245, 991, 1758, 2648, 2677
BmiI GGNNCC 2 cut(s) 475, 2678
BmrFI CCNGG 5 cut(s) 643, 813, 2259, 2537, 2652
BmsI GCATC 7 cut(s) 112, 741, 1598, 1971, 2086, 2108, 2425
BoxI GACNNNNGTC 1 cut(s) 672
BpiI GAAGAC 2 cut(s) 849, 2207
BplI GAGNNNNNCTC 2 cut(s) 1736, 1768
Bpu14I TTCGAA 1 cut(s) 982
BpuEI CTTGAG 5 cut(s) 482, 1322, 1384, 1961, 2645
Bsa29I ATCGAT 2 cut(s) 519, 555
BsaI GGTCTC 1 cut(s) 1196
BsaJI CCNNGG 7 cut(s) 248, 641, 642, 1285, 1521, 2392, 2644
BsaWI WCCGGW 2 cut(s) 538, 937
BsaXI ACNNNNNCTCC 6 cut(s) 1471, 1501, 1637, 1667, 2326, 2356
Bsc4I CCNNNNNNNGG 5 cut(s) 522, 692, 1286, 1996, 2472
Bse1I ACTGG 2 cut(s) 242, 337
Bse21I CCTNAGG 1 cut(s) 2214
Bse3DI GCAATG 1 cut(s) 2091
BseBI CCWGG 5 cut(s) 643, 813, 2259, 2537, 2652
BseCI ATCGAT 2 cut(s) 519, 555
BseDI CCNNGG 7 cut(s) 248, 641, 642, 1285, 1521, 2392, 2644
BseLI CCNNNNNNNGG 5 cut(s) 522, 692, 1286, 1996, 2472
BseMI GCAATG 1 cut(s) 2091
BseMII CTCAG 4 cut(s) 941, 1309, 2228, 2265
BseNI ACTGG 2 cut(s) 242, 337
BseRI GAGGAG 2 cut(s) 265, 1025
BseXI GCAGC 6 cut(s) 4, 435, 1899, 2159, 2444, 2578
BseYI CCCAGC 1 cut(s) 777
BsgI GTGCAG 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 444
BshFI GGCC 3 cut(s) 246, 1005, 2650
BshVI ATCGAT 2 cut(s) 519, 555
BsiHKAI GWGCWC 1 cut(s) 2391
BsiHKCI CYCGRG 3 cut(s) 247, 1418, 2643
BsiSI CCGG 2 cut(s) 539, 938
BslFI GGGAC 2 cut(s) 405, 1567
BslI CCNNNNNNNGG 5 cut(s) 522, 692, 1286, 1996, 2472
BsmAI GTCTC 6 cut(s) 584, 804, 963, 1019, 1196, 1340
BsmFI GGGAC 2 cut(s) 405, 1567
BsmI GAATGC 3 cut(s) 99, 2233, 2561
BsnI GGCC 3 cut(s) 246, 1005, 2650
Bso31I GGTCTC 1 cut(s) 1196
BsoBI CYCGRG 3 cut(s) 247, 1418, 2643
Bsp119I TTCGAA 1 cut(s) 982
Bsp1286I GDGCHC 1 cut(s) 2391
Bsp1407I TGTACA 1 cut(s) 2701
Bsp143I GATC 7 cut(s) 199, 261, 646, 1243, 1413, 1772, 2548
Bsp19I CCATGG 1 cut(s) 2392
BspACI CCGC 4 cut(s) 395, 444, 923, 2673
BspANI GGCC 3 cut(s) 246, 1005, 2650
BspCNI CTCAG 4 cut(s) 940, 1308, 2227, 2266
BspDI ATCGAT 2 cut(s) 519, 555
BspFNI CGCG 1 cut(s) 444
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 2 cut(s) 475, 2678
BspMAI CTGCAG 2 cut(s) 2177, 2596
BspPI GGATC 2 cut(s) 654, 1408
BspQI GCTCTTC 1 cut(s) 380
BspT104I TTCGAA 1 cut(s) 982
BspTI CTTAAG 2 cut(s) 1406, 1504
BspTNI GGTCTC 1 cut(s) 1196
BsrDI GCAATG 1 cut(s) 2091
BsrGI TGTACA 1 cut(s) 2701
BsrI ACTGG 2 cut(s) 242, 337
BssECI CCNNGG 7 cut(s) 248, 641, 642, 1285, 1521, 2392, 2644
BssMI GATC 7 cut(s) 199, 261, 646, 1243, 1413, 1772, 2548
BssT1I CCWWGG 3 cut(s) 1285, 1521, 2392
Bst2UI CCWGG 5 cut(s) 643, 813, 2259, 2537, 2652
Bst4CI ACNGT 6 cut(s) 156, 308, 747, 856, 1328, 2342
Bst6I CTCTTC 4 cut(s) 163, 360, 380, 1560
BstAFI CTTAAG 2 cut(s) 1406, 1504
BstAPI GCANNNNNTGC 2 cut(s) 137, 760
BstAUI TGTACA 1 cut(s) 2701
BstBI TTCGAA 1 cut(s) 982
BstC8I GCNNGC 6 cut(s) 442, 446, 622, 2082, 2414, 2508
BstDEI CTNAG 8 cut(s) 804, 927, 965, 1295, 1595, 2214, 2274, 2408
BstDSI CCRYGG 1 cut(s) 2392
BstFNI CGCG 1 cut(s) 444
BstH2I RGCGCY 1 cut(s) 1504
BstHHI GCGC 1 cut(s) 1503
BstKTI GATC 7 cut(s) 202, 264, 649, 1246, 1416, 1775, 2551
BstMAI GTCTC 6 cut(s) 584, 804, 963, 1019, 1196, 1340
BstMBI GATC 7 cut(s) 199, 261, 646, 1243, 1413, 1772, 2548
BstMWI GCNNNNNNNGC 5 cut(s) 137, 760, 1896, 2086, 2366
BstNI CCWGG 5 cut(s) 643, 813, 2259, 2537, 2652
BstNSI RCATGY 2 cut(s) 617, 624
BstPAI GACNNNNGTC 1 cut(s) 672
BstSCI CCNGG 5 cut(s) 641, 811, 2257, 2535, 2650
BstSFI CTRYAG 5 cut(s) 102, 563, 1686, 2173, 2592
BstUI CGCG 1 cut(s) 444
BstV1I GCAGC 6 cut(s) 4, 435, 1899, 2159, 2444, 2578
BstV2I GAAGAC 2 cut(s) 849, 2207
BstX2I RGATCY 2 cut(s) 1243, 1413
BstYI RGATCY 2 cut(s) 1243, 1413
Bsu15I ATCGAT 2 cut(s) 519, 555
Bsu36I CCTNAGG 1 cut(s) 2214
BsuI GTATCC 2 cut(s) 1043, 2581
BsuRI GGCC 3 cut(s) 246, 1005, 2650
BsuTUI ATCGAT 2 cut(s) 519, 555
BtgI CCRYGG 1 cut(s) 2392
BtgZI GCGATG 1 cut(s) 2061
BtsI GCAGTG 1 cut(s) 1481
BtsIMutI CAGTG 4 cut(s) 140, 852, 935, 1481
Cac8I GCNNGC 6 cut(s) 442, 446, 622, 2082, 2414, 2508
CaiI CAGNNNCTG 1 cut(s) 137
CciI TCATGA 1 cut(s) 232
CfoI GCGC 1 cut(s) 1503
Cfr13I GGNCC 6 cut(s) 45, 245, 991, 1758, 2648, 2677
ClaI ATCGAT 2 cut(s) 519, 555
Csp6I GTAC 3 cut(s) 173, 2378, 2702
CviQI GTAC 3 cut(s) 173, 2378, 2702
DdeI CTNAG 8 cut(s) 804, 927, 965, 1295, 1595, 2214, 2274, 2408
DpnI GATC 7 cut(s) 201, 263, 648, 1245, 1415, 1774, 2550
DpnII GATC 7 cut(s) 199, 261, 646, 1243, 1413, 1772, 2548
DraI TTTAAA 2 cut(s) 432, 2638
DraIII CACNNNGTG 1 cut(s) 856
Eam1104I CTCTTC 4 cut(s) 163, 360, 380, 1560
EarI CTCTTC 4 cut(s) 163, 360, 380, 1560
Eco130I CCWWGG 3 cut(s) 1285, 1521, 2392
Eco147I AGGCCT 1 cut(s) 1005
Eco31I GGTCTC 1 cut(s) 1196
Eco47I GGWCC 4 cut(s) 45, 991, 1758, 2677
Eco47III AGCGCT 1 cut(s) 1502
Eco57I CTGAAG 5 cut(s) 420, 881, 2134, 2219, 2678
Eco81I CCTNAGG 1 cut(s) 2214
Eco88I CYCGRG 3 cut(s) 247, 1418, 2643
EcoRI GAATTC 1 cut(s) 1272
EcoRII CCWGG 5 cut(s) 641, 811, 2257, 2535, 2650
EcoT14I CCWWGG 3 cut(s) 1285, 1521, 2392
EcoT22I ATGCAT 3 cut(s) 127, 756, 2101
ErhI CCWWGG 3 cut(s) 1285, 1521, 2392
FalI AAGNNNNNCTT 6 cut(s) 66, 98, 69, 101, 2224, 2256
FaqI GGGAC 2 cut(s) 405, 1567
FauI CCCGC 2 cut(s) 437, 2666
FauNDI CATATG 2 cut(s) 127, 298
FbaI TGATCA 1 cut(s) 261
FblI GTMKAC 1 cut(s) 101
Fnu4HI GCNGC 7 cut(s) 18, 449, 924, 1888, 2173, 2458, 2592
Fsp4HI GCNGC 7 cut(s) 18, 449, 924, 1888, 2173, 2458, 2592
FspBI CTAG 4 cut(s) 62, 584, 665, 1770
GlaI GCGC 1 cut(s) 1502
GluI GCNGC 7 cut(s) 18, 449, 924, 1888, 2173, 2458, 2592
GsaI CCCAGC 1 cut(s) 781
HaeII RGCGCY 1 cut(s) 1504
HaeIII GGCC 3 cut(s) 246, 1005, 2650
HapII CCGG 2 cut(s) 539, 938
HhaI GCGC 1 cut(s) 1503
Hin6I GCGC 1 cut(s) 1501
HinP1I GCGC 1 cut(s) 1501
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 3 cut(s) 1301, 1949, 1979
HpaII CCGG 2 cut(s) 539, 938
HphI GGTGA 6 cut(s) 314, 359, 910, 1883, 2543, 2674
Hpy166II GTNNAC 4 cut(s) 102, 712, 991, 2702
Hpy188I TCNGA 7 cut(s) 1087, 1298, 2029, 2153, 2548, 2578, 2658
Hpy8I GTNNAC 4 cut(s) 102, 712, 991, 2702
HpyCH4III ACNGT 6 cut(s) 156, 308, 747, 856, 1328, 2342
HpyCH4IV ACGT 4 cut(s) 192, 820, 1441, 2181
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 760, 1896, 2086, 2366
HpyF3I CTNAG 8 cut(s) 804, 927, 965, 1295, 1595, 2214, 2274, 2408
HpySE526I ACGT 4 cut(s) 192, 820, 1441, 2181
HspAI GCGC 1 cut(s) 1501
Ksp22I TGATCA 1 cut(s) 261
Kzo9I GATC 7 cut(s) 199, 261, 646, 1243, 1413, 1772, 2548
LguI GCTCTTC 1 cut(s) 380
LmnI GCTCC 2 cut(s) 1142, 1877
Lsp1109I GCAGC 6 cut(s) 4, 435, 1899, 2159, 2444, 2578
LweI GCATC 7 cut(s) 112, 741, 1598, 1971, 2086, 2108, 2425
MaeI CTAG 4 cut(s) 62, 584, 665, 1770
MaeII ACGT 4 cut(s) 192, 820, 1441, 2181
MaeIII GTNAC 4 cut(s) 302, 2177, 2319, 2336
MalI GATC 7 cut(s) 201, 263, 648, 1245, 1415, 1774, 2550
MboI GATC 7 cut(s) 199, 261, 646, 1243, 1413, 1772, 2548
MfeI CAATTG 4 cut(s) 209, 1494, 1858, 2143
MflI RGATCY 2 cut(s) 1243, 1413
MhlI GDGCHC 1 cut(s) 2391
MlyI GAGTC 3 cut(s) 806, 1351, 1821
MmeI TCCRAC 2 cut(s) 759, 1408
Mph1103I ATGCAT 3 cut(s) 127, 756, 2101
MroXI GAANNNNTTC 8 cut(s) 81, 354, 606, 786, 885, 1737, 2204, 2241
MslI CAYNNNNRTG 3 cut(s) 157, 899, 2292
MspCI CTTAAG 2 cut(s) 1406, 1504
MspI CCGG 2 cut(s) 539, 938
MspR9I CCNGG 5 cut(s) 643, 813, 2259, 2537, 2652
MunI CAATTG 4 cut(s) 209, 1494, 1858, 2143
Mva1269I GAATGC 3 cut(s) 99, 2233, 2561
MvaI CCWGG 5 cut(s) 643, 813, 2259, 2537, 2652
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 5 cut(s) 137, 760, 1896, 2086, 2366
NcoI CCATGG 1 cut(s) 2392
NdeI CATATG 2 cut(s) 127, 298
NdeII GATC 7 cut(s) 199, 261, 646, 1243, 1413, 1772, 2548
NlaIV GGNNCC 2 cut(s) 475, 2678
NmeAIII GCCGAG 1 cut(s) 818
NmuCI GTSAC 1 cut(s) 302
NsiI ATGCAT 3 cut(s) 127, 756, 2101
NspI RCATGY 2 cut(s) 617, 624
NspV TTCGAA 1 cut(s) 982
PaeI GCATGC 1 cut(s) 624
PagI TCATGA 1 cut(s) 232
PasI CCCWGGG 1 cut(s) 642
PceI AGGCCT 1 cut(s) 1005
PciI ACATGT 1 cut(s) 613
PciSI GCTCTTC 1 cut(s) 380
PctI GAATGC 3 cut(s) 99, 2233, 2561
PdmI GAANNNNTTC 8 cut(s) 81, 354, 606, 786, 885, 1737, 2204, 2241
PfeI GAWTC 7 cut(s) 350, 808, 1082, 1315, 1367, 1785, 1924
PflMI CCANNNNNTGG 1 cut(s) 1286
PfoI TCCNGGA 1 cut(s) 2257
PkrI GCNGC 7 cut(s) 19, 450, 925, 1889, 2174, 2459, 2593
PleI GAGTC 3 cut(s) 805, 1350, 1821
PpsI GAGTC 3 cut(s) 805, 1350, 1821
PscI ACATGT 1 cut(s) 613
PshAI GACNNNNGTC 1 cut(s) 672
PshBI ATTAAT 1 cut(s) 1569
PsiI TTATAA 1 cut(s) 630
Psp1406I AACGTT 1 cut(s) 1441
Psp6I CCWGG 5 cut(s) 641, 811, 2257, 2535, 2650
PspFI CCCAGC 1 cut(s) 777
PspGI CCWGG 5 cut(s) 641, 811, 2257, 2535, 2650
PspN4I GGNNCC 2 cut(s) 475, 2678
PspPI GGNCC 6 cut(s) 45, 245, 991, 1758, 2648, 2677
PstI CTGCAG 2 cut(s) 2177, 2596
PstNI CAGNNNCTG 1 cut(s) 137
PsuI RGATCY 2 cut(s) 1243, 1413
RsaI GTAC 3 cut(s) 174, 2379, 2703
RsaNI GTAC 3 cut(s) 173, 2378, 2702
RseI CAYNNNNRTG 3 cut(s) 157, 899, 2292
SapI GCTCTTC 1 cut(s) 380
SatI GCNGC 7 cut(s) 18, 449, 924, 1888, 2173, 2458, 2592
Sau3AI GATC 7 cut(s) 199, 261, 646, 1243, 1413, 1772, 2548
Sau96I GGNCC 6 cut(s) 45, 245, 991, 1758, 2648, 2677
ScaI AGTACT 1 cut(s) 174
SchI GAGTC 3 cut(s) 806, 1351, 1821
ScrFI CCNGG 5 cut(s) 643, 813, 2259, 2537, 2652
SduI GDGCHC 1 cut(s) 2391
SfaNI GCATC 7 cut(s) 112, 741, 1598, 1971, 2086, 2108, 2425
SfcI CTRYAG 5 cut(s) 102, 563, 1686, 2173, 2592
SfuI TTCGAA 1 cut(s) 982
SinI GGWCC 4 cut(s) 45, 991, 1758, 2677
SmiMI CAYNNNNRTG 3 cut(s) 157, 899, 2292
SmlI CTYRAG 7 cut(s) 461, 1337, 1399, 1406, 1504, 1976, 2624
SmoI CTYRAG 7 cut(s) 461, 1337, 1399, 1406, 1504, 1976, 2624
SphI GCATGC 1 cut(s) 624
SseBI AGGCCT 1 cut(s) 1005
SsiI CCGC 4 cut(s) 395, 444, 923, 2673
SspI AATATT 1 cut(s) 606
SspMI CTAG 4 cut(s) 62, 584, 665, 1770
StuI AGGCCT 1 cut(s) 1005
StyD4I CCNGG 5 cut(s) 641, 811, 2257, 2535, 2650
StyI CCWWGG 3 cut(s) 1285, 1521, 2392
TaaI ACNGT 6 cut(s) 156, 308, 747, 856, 1328, 2342
TaiI ACGT 4 cut(s) 195, 823, 1444, 2184
TaqII GACCGA 1 cut(s) 2273
TatI WGTACW 3 cut(s) 172, 2377, 2701
TauI GCSGC 1 cut(s) 926
TfiI GAWTC 7 cut(s) 350, 808, 1082, 1315, 1367, 1785, 1924
TscAI CASTG 4 cut(s) 147, 859, 935, 1488
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 6 cut(s) 17, 448, 1887, 2172, 2457, 2591
Tsp45I GTSAC 1 cut(s) 302
TspRI CASTG 4 cut(s) 147, 859, 935, 1488
Van91I CCANNNNNTGG 1 cut(s) 1286
Vha464I CTTAAG 2 cut(s) 1406, 1504
VpaK11BI GGWCC 4 cut(s) 45, 991, 1758, 2677
VspI ATTAAT 1 cut(s) 1569
XapI RAATTY 5 cut(s) 952, 978, 1272, 2268, 2527
XbaI TCTAGA 1 cut(s) 1769
XceI RCATGY 2 cut(s) 617, 624
XmiI GTMKAC 1 cut(s) 101
XmnI GAANNNNTTC 8 cut(s) 81, 354, 606, 786, 885, 1737, 2204, 2241
XspI CTAG 4 cut(s) 62, 584, 665, 1770
ZrmI AGTACT 1 cut(s) 174
Zsp2I ATGCAT 3 cut(s) 127, 756, 2101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.