RchiOBHm_Chr6g0250831

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000105
Physical Location & Seq
Forward (+)
6164991 .. 6168176
3186 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22484

Sequence Viewer

Length: 2688 bp
ATGATACAAAGACGATTAGGTAAATTAAGGGTGCTGGTTGTTATTGATGATGTGGATCAAGTGGCACAATTAGAATGTTTGGCTGGAAATCATGACTGGTTCGGTCCGGGAAGTAGAATTATCATAACCAGTGCTGATGTTCATTTGTTGAATTGTGTGGATACTCTATACGAGGCTGCTGTGTTAACCCATGATGAAGCTCTTCAACTTTTGAGTTTAAAGGCTTTTAAGAAAAACCCCCCGCCTGAAGTTTACTTGGAGCTATGCAACAATATTTTAGGGTATGCTCATGGGCTTCCATTAGTTCTTGTGGTTATAGGTCCATTTCTATGTGGTAGATCGATCAATGAATGGAAGAGTGCAATCGATAGGCTCAGGAATAAACCAGAAACTAGCATCGTTGATGTGCTTCAGATAAGTTTTGATGGGCTAAGGGAAACAGAAAAAGAAATTTTCCTACATGTCGCTTGCTTTTATGTGGGGAAGGATAGGGATCGTGTATCAGAAATACTAGATTATTGTCAGCTTTACCCTGACATTGGTTTAAGTGTTCTTGCTGATAAATCTCTCATTACTATTTCCAACAACAAACTATCCATGCATGCTTTGCTACAAGAAATGGGCTGGGAGATAGTTCGTAGGGAGTCGCCTAAACCAGGCAGGCGTACTAGATTATGGTCTCATGAAGACATCCACAATGTGCTGAAAAAAAATAAGGGAACAGAAGCAGTTCAAGGCATGGTAATGGACTTGCCTAAATTAGAAGTGGCTAATTGGAACCCCGAAGCATTCTCAAAGTTGTCTGAGCTTAACTTTCTCCAAATCCGTAATGTCGACCTTCCCAATGGCCTCACTTGCCTCTCTAATTCCTTGAGACTCCTCGAATGGAGTGAGTATCCCTTAAGATCTCTGCCACAAAATTTCGAAGCAGATGAACTTATTGAACTTAATTTGTGCCACAGCAAAATTGAACATCTTTGGGAGGGAACAAAGTGTTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAGAACATTGTCCAGACCCCAGACCTCACAGGCGCTTGCAGTCTTGAGTGTCTAGATCTTGAAGGGTGTAAAAACTTGGTAAAAATTCATCAATCCCATCAATCCGTTGGAGTTCTCAAAAAGCTTATTGTCTTGAATCTTAAAAACTGTGAGAGTCTGCAGAGTCTACCAGATAGAATTGAAATGGAGTCTCTTAAAACTCTTATTCTTTCTGGGTGTTCCAATATCAAGAAGATTCCTGAGTTTAGCAGAAATATGGAGTGTTTGTCAGAGCTGTGTCTTGATAGGACTGCCATTGAAAAACTGCCCGACTCAATCCAGTACCTATGTGGCCTGTCTTTCTTGGATCTAAGCAACTGCAAGAATCTTTTTTGTCTCCCAAGCACCATTAATAGATTGACAAGTCTTAAAAATATTAATCTTTCAGGATGCCAGAAGCTCAGGGGACCGGATAAAAGTGTGTGGGAGTTGGATTTTTTGCCGGCAAATGTTGCAAGTATAACTCCTGTAACAGAATTACCATCATCGGTCTTCAGCATTTCAGATCATGTTAAAGATTCAAGTTCTCATGGATATGAAGTCATTCAAGAATCATTGTTCGAGTTTTTGCCTTCTGGATTGATGCAAATGGCGTATGAAGAGCCCACGAGTTTCCGGTTGTCTTTATCTGGTCTGGGTAATTTAACAGATCTGAACATAAGTGACTGCAATCTTGATGACAGAGCATTTGTCAACAACTTTGGCTGGTTGCCCTGTTTAGTGGCATTAAATCTGAGTGGAAATGATTTTATTAAGCTTCCTCCAACCATCAGACGGCTTAATAAGCTTGAGAACGTTAACTTGGAGAACTGCAAAAGACTTGAAGACTTGTCAAATCTTCCATCAAATAGTAAGTTGGATGTAAGAGCTGATGGTTGCTCTTCATTGAGAATTTTGTTTGACATAACTAATTACAACAGACTGGAGGAATCATATTTCAGTTTCATCAACTGCTTTAAGCTAAACGAAGACCGAGGCCACAGTAATATAGCATTTGAAATGCTGAAGATAGTCCTTCATCAGGCTATCTCCACCACAAAGGAAACATTTCAAATTGTATTTCCTGGAAGTGAAATTCCTCGGTGGTTCAAACATCAAAGCCGTAAGGATTCATTGGGCGTATGCATACTACCTCCACGTCCATATTCCAGTAGGTTTATGGGATTTGCCTTGTGTGCTGTTTTTGTAATCAATGATGAGCGCCATCAGGTTGATGAACTTGAGATCCATCACTTCAAGACTTTTAAAGCTACGCATAATCTAGTCTGTTACCTGAAACTTAATGGAAAAGAATTAGAAGTATGTGGCAGACAGCCGGCCTTTCGGTTTAGTGAAGAATTTTGCCAGGTTGAGTCTGATCACCGTTGGGTATTCTACGTATCCTCGGATAAATACTTCGGTATTGAAGAGCATGACAGATGCTCTCAGATTGAACTGTTATTTGAAGCCAGAGGGCCAGGTTTGAAGGTGAAGGCGTGCGGGCTCCGTTTGATATTTGAACAAGATGTGCACGAGTTGATGAAGGGATGTAGACACACACAAGCTTACGGACGGCACGGATACGTACGGAGATATGAATGTGATTGTTGGGTGGATGGCTATTTATATTCTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

895

Amino Acids

102.04

Weight (kDa)

5.97

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 146 - 216 3.8e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 313 - 331 3e-06 Leucine Rich Repeat
LRR_14 PF23598 383 - 486 6.8e-08 Leucine-rich repeat region
LRR_13 PF23286 407 - 493 4.2e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 711 - 860 6.4e-16 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 834, 1198, 2602
AciI CCGC 2 cut(s) 242, 2550
AclI AACGTT 1 cut(s) 1866
AclWI GGATC 4 cut(s) 63, 501, 1386, 2290
AcsI RAATTY 6 cut(s) 450, 919, 1116, 1962, 2145, 2408
AcuI CTGAAG 4 cut(s) 267, 395, 1549, 2096
AdeI CACNNNGTG 1 cut(s) 700
AfaI GTAC 3 cut(s) 667, 1355, 2637
AfiI CCNNNNNNNGG 4 cut(s) 539, 656, 1845, 2092
AflII CTTAAG 1 cut(s) 901
AflIII ACRYGT 1 cut(s) 460
AjiI CACGTC 1 cut(s) 2210
AjnI CCWGG 4 cut(s) 655, 2134, 2415, 2527
AjuI GAANNNNNNNTTGG 2 cut(s) 1856, 1888
AloI GAACNNNNNNTCC 2 cut(s) 2280, 2312
Alw21I GWGCWC 1 cut(s) 2583
Alw26I GTCTC 4 cut(s) 684, 868, 1227, 1412
Alw44I GTGCAC 1 cut(s) 2579
AlwI GGATC 4 cut(s) 63, 501, 1386, 2290
AoxI GGCC 5 cut(s) 847, 1363, 2047, 2388, 2525
ApaLI GTGCAC 1 cut(s) 2579
ApeKI GCWGC 1 cut(s) 176
ApoI RAATTY 6 cut(s) 450, 919, 1116, 1962, 2145, 2408
ArsI GACNNNNNNTTYG 2 cut(s) 1742, 1774
AseI ATTAAT 2 cut(s) 1422, 1449
Asp700I GAANNNNTTC 4 cut(s) 201, 729, 1614, 2118
AspLEI GCGC 2 cut(s) 1067, 2274
AspS9I GGNCC 4 cut(s) 104, 320, 1478, 2525
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 2 cut(s) 2423, 2551
AsuII TTCGAA 1 cut(s) 924
AvaII GGWCC 3 cut(s) 104, 320, 1478
BaeGI GKGCMC 1 cut(s) 2583
BanII GRGCYC 2 cut(s) 1677, 2556
BauI CACGAG 2 cut(s) 1678, 2582
BbsI GAAGAC 4 cut(s) 693, 1555, 1902, 2046
Bbv12I GWGCWC 1 cut(s) 2583
BbvI GCAGC 1 cut(s) 163
BccI CCATC 9 cut(s) 419, 1137, 1561, 1847, 1921, 1937, 2283, 2307, 2660
BceAI ACGGC 3 cut(s) 1862, 2157, 2639
BciT130I CCWGG 4 cut(s) 657, 2136, 2417, 2529
BciVI GTATCC 4 cut(s) 154, 906, 2461, 2624
BclI TGATCA 1 cut(s) 2428
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 4 cut(s) 684, 868, 1227, 1412
BfaI CTAG 5 cut(s) 393, 512, 669, 1085, 2333
BfmI CTRYAG 1 cut(s) 1190
BfoI RGCGCY 2 cut(s) 1068, 2275
BfrI CTTAAG 1 cut(s) 901
BfuI GTATCC 4 cut(s) 154, 906, 2461, 2624
BglII AGATCT 3 cut(s) 905, 1087, 1720
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 5 cut(s) 108, 657, 2136, 2417, 2529
Bme18I GGWCC 3 cut(s) 104, 320, 1478
BmgBI CACGTC 1 cut(s) 2210
BmgT120I GGNCC 4 cut(s) 104, 320, 1478, 2525
BmiI GGNNCC 3 cut(s) 779, 1479, 2555
BmrFI CCNGG 5 cut(s) 108, 657, 2136, 2417, 2529
BmsI GCATC 4 cut(s) 405, 1451, 1644, 2480
BpiI GAAGAC 4 cut(s) 693, 1555, 1902, 2046
BpmI CTGGAG 1 cut(s) 2015
Bpu10I CCTNAGC 3 cut(s) 374, 431, 1472
Bpu14I TTCGAA 1 cut(s) 924
BpuEI CTTGAG 4 cut(s) 892, 1097, 1880, 2312
BpuMI CCSGG 1 cut(s) 108
Bsa29I ATCGAT 2 cut(s) 341, 366
BsaAI YACGTR 2 cut(s) 2449, 2635
BsaBI GATNNNNATC 2 cut(s) 54, 492
BsaI GGTCTC 1 cut(s) 684
BsaJI CCNNGG 3 cut(s) 2044, 2150, 2454
BsaWI WCCGGW 2 cut(s) 1480, 1686
BsaXI ACNNNNNCTCC 2 cut(s) 1283, 1313
Bsc4I CCNNNNNNNGG 4 cut(s) 539, 656, 1845, 2092
Bse118I RCCGGY 2 cut(s) 1513, 2386
Bse1I ACTGG 5 cut(s) 101, 129, 1351, 1998, 2220
Bse8I GATNNNNATC 2 cut(s) 54, 492
BseBI CCWGG 4 cut(s) 657, 2136, 2417, 2529
BseCI ATCGAT 2 cut(s) 341, 366
BseDI CCNNGG 3 cut(s) 2044, 2150, 2454
BseGI GGATG 5 cut(s) 690, 1466, 1936, 2603, 2671
BseJI GATNNNNATC 2 cut(s) 54, 492
BseLI CCNNNNNNNGG 4 cut(s) 539, 656, 1845, 2092
BseMII CTCAG 7 cut(s) 388, 795, 1046, 1263, 1486, 1796, 2510
BseNI ACTGG 5 cut(s) 101, 129, 1351, 1998, 2220
BseRI GAGGAG 1 cut(s) 869
BseSI GKGCMC 1 cut(s) 2583
BseXI GCAGC 1 cut(s) 163
BseYI CCCAGC 1 cut(s) 624
BshFI GGCC 5 cut(s) 849, 1365, 2049, 2390, 2527
BshVI ATCGAT 2 cut(s) 341, 366
BsiHKAI GWGCWC 1 cut(s) 2583
BsiSI CCGG 5 cut(s) 107, 1481, 1514, 1687, 2387
BsiWI CGTACG 1 cut(s) 2635
BslFI GGGAC 1 cut(s) 1491
BslI CCNNNNNNNGG 4 cut(s) 539, 656, 1845, 2092
BsmAI GTCTC 4 cut(s) 684, 868, 1227, 1412
BsmFI GGGAC 1 cut(s) 1491
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 5 cut(s) 849, 1365, 2049, 2390, 2527
Bso31I GGTCTC 1 cut(s) 684
Bsp119I TTCGAA 1 cut(s) 924
Bsp1286I GDGCHC 3 cut(s) 1677, 2556, 2583
BspACI CCGC 2 cut(s) 242, 2550
BspANI GGCC 5 cut(s) 849, 1365, 2049, 2390, 2527
BspCNI CTCAG 7 cut(s) 387, 796, 1045, 1264, 1485, 1797, 2509
BspDI ATCGAT 2 cut(s) 341, 366
BspHI TCATGA 2 cut(s) 91, 682
BspLI GGNNCC 3 cut(s) 779, 1479, 2555
BspMAI CTGCAG 1 cut(s) 1194
BspPI GGATC 4 cut(s) 63, 501, 1386, 2290
BspQI GCTCTTC 4 cut(s) 207, 1665, 1957, 2472
BspT104I TTCGAA 1 cut(s) 924
BspTI CTTAAG 1 cut(s) 901
BspTNI GGTCTC 1 cut(s) 684
BsrFI RCCGGY 2 cut(s) 1513, 2386
BsrI ACTGG 5 cut(s) 101, 129, 1351, 1998, 2220
BssAI RCCGGY 2 cut(s) 1513, 2386
BssECI CCNNGG 3 cut(s) 2044, 2150, 2454
BssSI CACGAG 2 cut(s) 1678, 2582
Bst2BI CACGAG 2 cut(s) 1678, 2582
Bst2UI CCWGG 4 cut(s) 657, 2136, 2417, 2529
Bst4CI ACNGT 4 cut(s) 1181, 2054, 2435, 2508
Bst6I CTCTTC 5 cut(s) 207, 350, 1665, 1957, 2472
BstAFI CTTAAG 1 cut(s) 901
BstAPI GCANNNNNTGC 2 cut(s) 607, 1523
BstBAI YACGTR 2 cut(s) 2449, 2635
BstBI TTCGAA 1 cut(s) 924
BstC8I GCNNGC 8 cut(s) 469, 603, 662, 1069, 1515, 2388, 2548, 2552
BstDEI CTNAG 9 cut(s) 374, 431, 804, 1032, 1272, 1382, 1472, 1805, 2496
BstENI CCTNNNNNAGG 2 cut(s) 654, 2090
BstF5I GGATG 5 cut(s) 690, 1466, 1936, 2603, 2671
BstH2I RGCGCY 2 cut(s) 1068, 2275
BstHHI GCGC 2 cut(s) 1067, 2274
BstMAI GTCTC 4 cut(s) 684, 868, 1227, 1412
BstMWI GCNNNNNNNGC 5 cut(s) 607, 855, 1523, 1855, 2246
BstNI CCWGG 4 cut(s) 657, 2136, 2417, 2529
BstNSI RCATGY 2 cut(s) 464, 605
BstSCI CCNGG 5 cut(s) 106, 655, 2134, 2415, 2527
BstSFI CTRYAG 1 cut(s) 1190
BstSLI GKGCMC 1 cut(s) 2583
BstSNI TACGTA 2 cut(s) 2449, 2635
BstV1I GCAGC 1 cut(s) 163
BstV2I GAAGAC 4 cut(s) 693, 1555, 1902, 2046
BstX2I RGATCY 5 cut(s) 905, 1087, 1378, 1720, 2295
BstYI RGATCY 5 cut(s) 905, 1087, 1378, 1720, 2295
Bsu15I ATCGAT 2 cut(s) 341, 366
BsuI GTATCC 4 cut(s) 154, 906, 2461, 2624
BsuRI GGCC 5 cut(s) 849, 1365, 2049, 2390, 2527
BsuTUI ATCGAT 2 cut(s) 341, 366
BtrI CACGTC 1 cut(s) 2210
BtsCI GGATG 5 cut(s) 690, 1466, 1936, 2603, 2671
BtsIMutI CAGTG 1 cut(s) 136
Cac8I GCNNGC 8 cut(s) 469, 603, 662, 1069, 1515, 2388, 2548, 2552
CciI TCATGA 2 cut(s) 91, 682
CfoI GCGC 2 cut(s) 1067, 2274
Cfr10I RCCGGY 2 cut(s) 1513, 2386
Cfr13I GGNCC 4 cut(s) 104, 320, 1478, 2525
ClaI ATCGAT 2 cut(s) 341, 366
CpoI CGGWCCG 1 cut(s) 104
Csp6I GTAC 3 cut(s) 666, 1354, 2636
CspI CGGWCCG 1 cut(s) 104
CviQI GTAC 3 cut(s) 666, 1354, 2636
DdeI CTNAG 9 cut(s) 374, 431, 804, 1032, 1272, 1382, 1472, 1805, 2496
DraI TTTAAA 2 cut(s) 219, 2317
DraIII CACNNNGTG 1 cut(s) 700
Eam1104I CTCTTC 5 cut(s) 207, 350, 1665, 1957, 2472
EarI CTCTTC 5 cut(s) 207, 350, 1665, 1957, 2472
Eco105I TACGTA 2 cut(s) 2449, 2635
Eco24I GRGCYC 2 cut(s) 1677, 2556
Eco31I GGTCTC 1 cut(s) 684
Eco47I GGWCC 3 cut(s) 104, 320, 1478
Eco57I CTGAAG 4 cut(s) 267, 395, 1549, 2096
EcoNI CCTNNNNNAGG 2 cut(s) 654, 2090
EcoRII CCWGG 4 cut(s) 655, 2134, 2415, 2527
EcoT22I ATGCAT 2 cut(s) 603, 2198
EcoT38I GRGCYC 2 cut(s) 1677, 2556
FaqI GGGAC 1 cut(s) 1491
FauI CCCGC 2 cut(s) 249, 2543
FbaI TGATCA 1 cut(s) 2428
FblI GTMKAC 3 cut(s) 834, 1198, 2602
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 5 cut(s) 677, 1473, 1943, 2610, 2678
FriOI GRGCYC 2 cut(s) 1677, 2556
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 5 cut(s) 393, 512, 669, 1085, 2333
GlaI GCGC 2 cut(s) 1066, 2273
GluI GCNGC 1 cut(s) 177
GsaI CCCAGC 1 cut(s) 628
GsuI CTGGAG 1 cut(s) 2015
HaeII RGCGCY 2 cut(s) 1068, 2275
HaeIII GGCC 5 cut(s) 849, 1365, 2049, 2390, 2527
HapII CCGG 5 cut(s) 107, 1481, 1514, 1687, 2387
HhaI GCGC 2 cut(s) 1067, 2274
Hin6I GCGC 2 cut(s) 1065, 2272
HinP1I GCGC 2 cut(s) 1065, 2272
HincII GTYRAC 4 cut(s) 186, 835, 1765, 1870
HindII GTYRAC 4 cut(s) 186, 835, 1765, 1870
HindIII AAGCTT 4 cut(s) 1154, 1826, 1856, 2613
HpaI GTTAAC 2 cut(s) 186, 1870
HpaII CCGG 5 cut(s) 107, 1481, 1514, 1687, 2387
HphI GGTGA 2 cut(s) 2423, 2551
Hpy166II GTNNAC 8 cut(s) 186, 253, 835, 1199, 1765, 1870, 2581, 2603
Hpy8I GTNNAC 8 cut(s) 186, 253, 835, 1199, 1765, 1870, 2581, 2603
HpyAV CCTTC 8 cut(s) 478, 848, 1088, 1653, 2096, 2530, 2536, 2587
HpyCH4III ACNGT 4 cut(s) 1181, 2054, 2435, 2508
HpyCH4IV ACGT 4 cut(s) 1866, 2209, 2448, 2634
HpyF10VI GCNNNNNNNGC 5 cut(s) 607, 855, 1523, 1855, 2246
HpyF3I CTNAG 9 cut(s) 374, 431, 804, 1032, 1272, 1382, 1472, 1805, 2496
HpySE526I ACGT 4 cut(s) 1866, 2209, 2448, 2634
HspAI GCGC 2 cut(s) 1065, 2272
KroI GCCGGC 2 cut(s) 1513, 2386
KroNI GCCGGC 2 cut(s) 1515, 2388
Ksp22I TGATCA 1 cut(s) 2428
KspAI GTTAAC 2 cut(s) 186, 1870
LguI GCTCTTC 4 cut(s) 207, 1665, 1957, 2472
LmnI GCTCC 2 cut(s) 259, 2559
Lsp1109I GCAGC 1 cut(s) 163
LweI GCATC 4 cut(s) 405, 1451, 1644, 2480
MaeI CTAG 5 cut(s) 393, 512, 669, 1085, 2333
MaeII ACGT 4 cut(s) 1866, 2209, 2448, 2634
MaeIII GTNAC 3 cut(s) 1540, 1733, 2339
MflI RGATCY 5 cut(s) 905, 1087, 1378, 1720, 2295
MhlI GDGCHC 3 cut(s) 1677, 2556, 2583
MlyI GAGTC 7 cut(s) 653, 870, 1195, 1204, 1229, 1337, 2432
MmeI TCCRAC 5 cut(s) 606, 1120, 1482, 1859, 1908
Mph1103I ATGCAT 2 cut(s) 603, 2198
MroNI GCCGGC 2 cut(s) 1513, 2386
MroXI GAANNNNTTC 4 cut(s) 201, 729, 1614, 2118
MslI CAYNNNNRTG 3 cut(s) 328, 743, 1605
MspCI CTTAAG 1 cut(s) 901
MspI CCGG 5 cut(s) 107, 1481, 1514, 1687, 2387
MspR9I CCNGG 5 cut(s) 108, 657, 2136, 2417, 2529
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 4 cut(s) 657, 2136, 2417, 2529
MwoI GCNNNNNNNGC 5 cut(s) 607, 855, 1523, 1855, 2246
NaeI GCCGGC 2 cut(s) 1515, 2388
NciI CCSGG 1 cut(s) 108
NgoMIV GCCGGC 2 cut(s) 1513, 2386
NlaIV GGNNCC 3 cut(s) 779, 1479, 2555
NmuCI GTSAC 1 cut(s) 1733
NsiI ATGCAT 2 cut(s) 603, 2198
NspI RCATGY 2 cut(s) 464, 605
NspV TTCGAA 1 cut(s) 924
PaeI GCATGC 1 cut(s) 605
PagI TCATGA 2 cut(s) 91, 682
PciI ACATGT 1 cut(s) 460
PciSI GCTCTTC 4 cut(s) 207, 1665, 1957, 2472
PctI GAATGC 1 cut(s) 788
PdiI GCCGGC 2 cut(s) 1515, 2388
PdmI GAANNNNTTC 4 cut(s) 201, 729, 1614, 2118
PfeI GAWTC 7 cut(s) 1168, 1267, 1396, 1589, 1622, 2000, 2180
Pfl23II CGTACG 1 cut(s) 2635
PfoI TCCNGGA 2 cut(s) 106, 2134
PkrI GCNGC 1 cut(s) 178
PleI GAGTC 7 cut(s) 652, 870, 1194, 1203, 1228, 1337, 2431
PpsI GAGTC 7 cut(s) 652, 870, 1194, 1203, 1228, 1337, 2431
Ppu21I YACGTR 2 cut(s) 2449, 2635
PscI ACATGT 1 cut(s) 460
PshBI ATTAAT 2 cut(s) 1422, 1449
Psp1406I AACGTT 1 cut(s) 1866
Psp6I CCWGG 4 cut(s) 655, 2134, 2415, 2527
PspFI CCCAGC 1 cut(s) 624
PspGI CCWGG 4 cut(s) 655, 2134, 2415, 2527
PspLI CGTACG 1 cut(s) 2635
PspN4I GGNNCC 3 cut(s) 779, 1479, 2555
PspPI GGNCC 4 cut(s) 104, 320, 1478, 2525
PstI CTGCAG 1 cut(s) 1194
PsuI RGATCY 5 cut(s) 905, 1087, 1378, 1720, 2295
RsaI GTAC 3 cut(s) 667, 1355, 2637
RsaNI GTAC 3 cut(s) 666, 1354, 2636
RseI CAYNNNNRTG 3 cut(s) 328, 743, 1605
Rsr2I CGGWCCG 1 cut(s) 104
RsrII CGGWCCG 1 cut(s) 104
SalI GTCGAC 1 cut(s) 833
SapI GCTCTTC 4 cut(s) 207, 1665, 1957, 2472
SatI GCNGC 1 cut(s) 177
Sau96I GGNCC 4 cut(s) 104, 320, 1478, 2525
SchI GAGTC 7 cut(s) 653, 870, 1195, 1204, 1229, 1337, 2432
ScrFI CCNGG 5 cut(s) 108, 657, 2136, 2417, 2529
SduI GDGCHC 3 cut(s) 1677, 2556, 2583
SfaNI GCATC 4 cut(s) 405, 1451, 1644, 2480
SfcI CTRYAG 1 cut(s) 1190
SfuI TTCGAA 1 cut(s) 924
SinI GGWCC 3 cut(s) 104, 320, 1478
SmiMI CAYNNNNRTG 3 cut(s) 328, 743, 1605
SmlI CTYRAG 5 cut(s) 871, 901, 1076, 1859, 2291
SmoI CTYRAG 5 cut(s) 871, 901, 1076, 1859, 2291
SnaBI TACGTA 2 cut(s) 2449, 2635
SphI GCATGC 1 cut(s) 605
SsiI CCGC 2 cut(s) 242, 2550
SspI AATATT 2 cut(s) 274, 1447
SspMI CTAG 5 cut(s) 393, 512, 669, 1085, 2333
StyD4I CCNGG 5 cut(s) 106, 655, 2134, 2415, 2527
TaaI ACNGT 4 cut(s) 1181, 2054, 2435, 2508
TaiI ACGT 4 cut(s) 1869, 2212, 2451, 2637
TaqI TCGA 6 cut(s) 341, 366, 834, 882, 924, 1632
TaqII GACCGA 3 cut(s) 92, 1549, 2058
TfiI GAWTC 7 cut(s) 1168, 1267, 1396, 1589, 1622, 2000, 2180
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 1 cut(s) 1733
TseI GCWGC 1 cut(s) 176
Tsp45I GTSAC 1 cut(s) 1733
TspGWI ACGGA 6 cut(s) 815, 1126, 2546, 2634, 2643, 2653
TspRI CASTG 1 cut(s) 136
Vha464I CTTAAG 1 cut(s) 901
VneI GTGCAC 1 cut(s) 2579
VpaK11BI GGWCC 3 cut(s) 104, 320, 1478
VspI ATTAAT 2 cut(s) 1422, 1449
XagI CCTNNNNNAGG 2 cut(s) 654, 2090
XapI RAATTY 6 cut(s) 450, 919, 1116, 1962, 2145, 2408
XbaI TCTAGA 1 cut(s) 1084
XceI RCATGY 2 cut(s) 464, 605
XcmI CCANNNNNNNNNTGG 3 cut(s) 1136, 1358, 2227
XmiI GTMKAC 3 cut(s) 834, 1198, 2602
XmnI GAANNNNTTC 4 cut(s) 201, 729, 1614, 2118
XspI CTAG 5 cut(s) 393, 512, 669, 1085, 2333
Zsp2I ATGCAT 2 cut(s) 603, 2198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.