Rw1G004470

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Reverse (-)
8508392 .. 8512672
4281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004470.1

Sequence Viewer

Length: 3633 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTCTTTCTAAGTTTCAGGGGGGAAGATACGCGCTGGTCTTTTACCGAGTATCTGTATATTGTACTAAATCAGAGAGGAATTCTCACCTTTCGGGATGACCGAGAGCTTGAGAAAGGAAAATCCATTAGGCCCGAACTTTTAGGTGCAATTAAGGAGTCTAGATCTGCGATTGTCATTCTCTCACCCAGATATGGTCGTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGATGGGCCAACAAGTCTTCCCCATCTTCTACGGCGTGAATCCTTCTGATGTGCGGCACCAAACGGGATGTTTTGAGCTCAAATGGATATCCCAAGTAGATGTGCAAGTAAGGGAACATGAAGAAGTTTATGGGAAGTATAATGAGGACAGAATAAATGCCTGGAGAGCTGCTTTGGCTGAGGTCGCCAATCTTTCTGGCTGGGAATTCAACTATTATGGAAGTGTAACAAAACTAATTGAGGAAATTGCTAATCATATGCTAAAGATGCCCGTGCAGACACCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGGTTCCCGCATTGATGATTTATTAAGCAATTACATATATCCGCAGCTTGATGCGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATTTGTCAGGATTTTGACCGAGCCTACTTTCTTTCCAATGTTAGAGAAAGGTCTAAAAACAATGGCCTAGTTTCTCTTCAAGAAAAACTTCTTTCTGAAATCCTAATGGCAAAAATTAAAAATATAGCGGATGAATACACAGGAGCTGCTATGATAAAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCACTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTTCTGGGAGTAGAATTGTCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGCTGTTGATGCTACATACAAGGCTAACGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATATCCCCCACCAGCAGATTATTTGCATCTGTGCTACGATATTATAGGGTATGCCCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGAACTGATGAATGGGCAAGTGCGATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGATGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAGATCTCTCATAACTCTCTCCAACAACGAACTGTGGATGCATGATTTGCTGCAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCCCAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGACGACATCAACAATGTGCTGAAGAAAAATAGGGGAACGGATTCAATCCAAGGCATGGTAATCGAGTTGACTGAATTACAAGTGGCTCATTGGAATCCAGAAGCCTTTTCAAATTTGTCTCAGCTTGGTCTTCTCCATATTCGTAATGTGGACCTTCCCAAAGGCCTCAATTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTAAGATCTCTCCCAAAATTTTTTGAAGGAGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTAAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAGCATTGTGGAGACCCCAGACCTCGCAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGGTGTGAGAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCTGAGTTTGTTGGAAATATGGAACGTTTGTCGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCAGTATCAATTGAACGGCTGACTGGCCTTGTGACATTGAATCTGAGCAACTGCAGAAATCTTGTCTGTCTTCCTAGCACCGTCGATATATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAACGTGGGGGTGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTCCGCAATAGAAATGTCTCCTACCCATGATCGCAGAAAATATGTGAGAGGTTCAATCTTTCATGGATGCAAAGTAGTTTGGCGATCTTTAAAAAAGGTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAAAGAGTTTCCACTTGCCTATAGCACAAAAAGTGAACACAGAGCCAATGAGTTTCCGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCCACTTTGGTGACCTTGAATCTAAGTGGGAACAATTTTGTTCGTCTTCCTTCAGGCATTGGATTACTTTCTAGGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGGACAAATTAACCAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTAATGTATCAGGGAATCTCTAATGACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCAGAGTGTTGGGTGTTCTGTAAGTGTATCTCTGCCTGCACATTGGAATAATAGTCGGGTTTTGGGATTTGCTCTGTGTGCTGTTTTTGTACTCCATGAGCACCATCGGGTGGATGAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTCAAGCTCGATGGAAGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCAAGGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTCATGATAAATACTTTGGTACAGAGCGGTGGCATAACAGTTGCAGTCAGTTTGAGTTCTTATTTGAAACCAGAGGGCCAGGTCTGAAGGTGAAGGAGTGCGGAGTCCGTCTGATATATGAGCAAGATGTGCAAGAGTTGAACCGAACAACCACTTCATCAAGCAGTGGGATGTCTCCTTATGGGGATATATTGATTGGTTTTGACATTCCAGTTGCAGGGGAAACCAGTGGCAGTGGTAGCAGAACTTGCATGCTTGAAGAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1210

Amino Acids

137.37

Weight (kDa)

6.41

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 192 2.8e-46 TIR domain
TIR_2 PF13676 14 - 109 9.1e-13 TIR domain
NB-ARC PF00931 218 - 372 2.3e-18 NB-ARC domain
NACHT PF05729 222 - 320 7.8e-06 NACHT domain
WHD_ROQ1 PF23282 439 - 510 5.4e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 639 - 732 1.1e-06 Leucine-rich repeat region
LRR_13 PF23286 699 - 784 1.1e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 710 - 778 7.7e-08 Leucine-rich repeat region
C-JID PF20160 1017 - 1166 8.3e-21 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 2 cut(s) 1624, 3193
AccBSI CCGCTC 1 cut(s) 3391
AccI GTMKAC 1 cut(s) 2842
AccII CGCG 1 cut(s) 70
AclI AACGTT 1 cut(s) 2170
AclWI GGATC 4 cut(s) 664, 677, 939, 1383
AcuI CTGAAG 4 cut(s) 1610, 2736, 3014, 3470
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 102, 2869, 3174, 3385
AfiI CCNNNNNNNGG 6 cut(s) 230, 1421, 1624, 2618, 3193, 3581
AflII CTTAAG 1 cut(s) 1786
AjnI CCWGG 5 cut(s) 446, 1170, 1540, 3052, 3442
AjuI GAANNNNNNNTTGG 4 cut(s) 2774, 2806, 2909, 2941
AleI CACNNNNGTG 1 cut(s) 2708
Alw21I GWGCWC 2 cut(s) 366, 3186
AlwI GGATC 4 cut(s) 664, 677, 939, 1383
AlwNI CAGNNNCTG 1 cut(s) 872
Ama87I CYCGRG 2 cut(s) 708, 2066
AoxI GGCC 6 cut(s) 167, 292, 790, 1732, 2239, 3440
ApeKI GCWGC 4 cut(s) 455, 643, 872, 1492
AseI ATTAAT 2 cut(s) 2111, 3002
Asp700I GAANNNNTTC 6 cut(s) 601, 813, 1083, 1515, 1609, 3036
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 5 cut(s) 168, 292, 1720, 2412, 3440
AsuHPI GGTGA 7 cut(s) 115, 213, 2386, 2678, 2722, 3335, 3466
AvaI CYCGRG 2 cut(s) 708, 2066
AvaII GGWCC 2 cut(s) 1720, 2412
BamHI GGATCC 1 cut(s) 669
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 1190
BbsI GAAGAC 5 cut(s) 295, 1691, 2276, 2738, 3002
Bbv12I GWGCWC 2 cut(s) 366, 3186
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 4 cut(s) 442, 655, 859, 1479
BceAI ACGGC 2 cut(s) 334, 2246
BcgI CGANNNNNNTGC 4 cut(s) 1612, 1646, 2047, 2081
BciT130I CCWGG 5 cut(s) 448, 1172, 1542, 3054, 3444
BciVI GTATCC 1 cut(s) 1791
BfaI CTAG 6 cut(s) 198, 794, 1394, 2289, 2772, 2846
BfmI CTRYAG 2 cut(s) 2266, 2568
BfrI CTTAAG 1 cut(s) 1786
BfuI GTATCC 1 cut(s) 1791
BglII AGATCT 4 cut(s) 200, 1445, 1790, 1972
BisI GCNGC 5 cut(s) 341, 456, 644, 873, 1493
BlsI GCNGC 5 cut(s) 342, 457, 645, 874, 1494
Bme1390I CCNGG 5 cut(s) 448, 1172, 1542, 3054, 3444
Bme18I GGWCC 2 cut(s) 1720, 2412
BmeT110I CYCGRG 2 cut(s) 708, 2066
BmgT120I GGNCC 5 cut(s) 168, 292, 1720, 2412, 3440
BmiI GGNNCC 4 cut(s) 344, 604, 671, 2413
BmrFI CCNGG 5 cut(s) 448, 1172, 1542, 3054, 3444
BmrI ACTGGG 1 cut(s) 1785
BmuI ACTGGG 1 cut(s) 1785
BpiI GAAGAC 5 cut(s) 295, 1691, 2276, 2738, 3002
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 1 cut(s) 469
Bpu10I CCTNAGC 1 cut(s) 465
BpuEI CTTGAG 5 cut(s) 167, 1777, 1982, 2798, 3242
BsaBI GATNNNNATC 2 cut(s) 930, 1374
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1569, 1925
BsaJI CCNNGG 5 cut(s) 673, 1170, 1171, 1618, 2014
Bsc4I CCNNNNNNNGG 6 cut(s) 230, 1421, 1624, 2618, 3193, 3581
Bse1I ACTGG 7 cut(s) 977, 1317, 1780, 2216, 2242, 3575, 3591
Bse8I GATNNNNATC 2 cut(s) 930, 1374
BseBI CCWGG 5 cut(s) 448, 1172, 1542, 3054, 3444
BseDI CCNNGG 5 cut(s) 673, 1170, 1171, 1618, 2014
BseJI GATNNNNATC 2 cut(s) 930, 1374
BseLI CCNNNNNNNGG 6 cut(s) 230, 1421, 1624, 2618, 3193, 3581
BseMII CTCAG 6 cut(s) 456, 1703, 2139, 2249, 2639, 3060
BseNI ACTGG 7 cut(s) 977, 1317, 1780, 2216, 2242, 3575, 3591
BseRI GAGGAG 1 cut(s) 1754
BseXI GCAGC 4 cut(s) 442, 655, 859, 1479
BseYI CCCAGC 2 cut(s) 486, 1506
BsgI GTGCAG 2 cut(s) 581, 3105
Bsh1236I CGCG 1 cut(s) 70
BshFI GGCC 6 cut(s) 169, 294, 792, 1734, 2241, 3442
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 2 cut(s) 366, 3186
BsiHKCI CYCGRG 2 cut(s) 708, 2066
BslI CCNNNNNNNGG 6 cut(s) 230, 1421, 1624, 2618, 3193, 3581
BsnI GGCC 6 cut(s) 169, 294, 792, 1734, 2241, 3442
Bso31I GGTCTC 2 cut(s) 1569, 1925
BsoBI CYCGRG 2 cut(s) 708, 2066
Bsp1286I GDGCHC 2 cut(s) 366, 3186
Bsp19I CCATGG 1 cut(s) 673
BspANI GGCC 6 cut(s) 169, 294, 792, 1734, 2241, 3442
BspCNI CTCAG 6 cut(s) 457, 1702, 2140, 2250, 2640, 3061
BspFNI CGCG 1 cut(s) 70
BspHI TCATGA 2 cut(s) 1567, 3367
BspLI GGNNCC 4 cut(s) 344, 604, 671, 2413
BspMAI CTGCAG 1 cut(s) 2270
BspPI GGATC 4 cut(s) 664, 677, 939, 1383
BspT107I GGYRCC 1 cut(s) 342
BspTI CTTAAG 1 cut(s) 1786
BspTNI GGTCTC 2 cut(s) 1569, 1925
BsrBI CCGCTC 1 cut(s) 3391
BsrI ACTGG 7 cut(s) 977, 1317, 1780, 2216, 2242, 3575, 3591
BssECI CCNNGG 5 cut(s) 673, 1170, 1171, 1618, 2014
BssNAI GTATAC 1 cut(s) 2843
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1190
BssT1I CCWWGG 3 cut(s) 673, 1618, 2014
Bst1107I GTATAC 1 cut(s) 2843
Bst2BI CACGAG 1 cut(s) 1190
Bst2UI CCWGG 5 cut(s) 448, 1172, 1542, 3054, 3444
Bst4CI ACNGT 6 cut(s) 1073, 1476, 1527, 2296, 2384, 3404
Bst6I CTCTTC 3 cut(s) 19, 807, 3265
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1786
BstAPI GCANNNNNTGC 3 cut(s) 1489, 3493, 3612
BstC8I GCNNGC 8 cut(s) 680, 1351, 1406, 1948, 2609, 3120, 3300, 3617
BstDEI CTNAG 8 cut(s) 47, 465, 1689, 2148, 2258, 2648, 2723, 3069
BstDSI CCRYGG 1 cut(s) 673
BstEII GGTNACC 1 cut(s) 2710
BstFNI CGCG 1 cut(s) 70
BstHHI GCGC 1 cut(s) 72
BstNI CCWGG 5 cut(s) 448, 1172, 1542, 3054, 3444
BstNSI RCATGY 3 cut(s) 682, 1037, 3619
BstPI GGTNACC 1 cut(s) 2710
BstSCI CCNGG 5 cut(s) 446, 1170, 1540, 3052, 3442
BstSFI CTRYAG 2 cut(s) 2266, 2568
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 4 cut(s) 442, 655, 859, 1479
BstV2I GAAGAC 5 cut(s) 295, 1691, 2276, 2738, 3002
BstX2I RGATCY 5 cut(s) 200, 669, 1445, 1790, 1972
BstYI RGATCY 5 cut(s) 200, 669, 1445, 1790, 1972
BstZ17I GTATAC 1 cut(s) 2843
BsuI GTATCC 1 cut(s) 1791
BsuRI GGCC 6 cut(s) 169, 294, 792, 1734, 2241, 3442
BtgI CCRYGG 1 cut(s) 673
BtsI GCAGTG 2 cut(s) 3535, 3604
BtsIMutI CAGTG 4 cut(s) 2873, 3535, 3598, 3604
Cac8I GCNNGC 8 cut(s) 680, 1351, 1406, 1948, 2609, 3120, 3300, 3617
CaiI CAGNNNCTG 1 cut(s) 872
CciI TCATGA 2 cut(s) 1567, 3367
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 5 cut(s) 168, 292, 1720, 2412, 3440
Csp6I GTAC 4 cut(s) 101, 2868, 3173, 3384
CviQI GTAC 4 cut(s) 101, 2868, 3173, 3384
DdeI CTNAG 8 cut(s) 47, 465, 1689, 2148, 2258, 2648, 2723, 3069
DraI TTTAAA 1 cut(s) 2502
Eam1104I CTCTTC 3 cut(s) 19, 807, 3265
EarI CTCTTC 3 cut(s) 19, 807, 3265
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 3 cut(s) 673, 1618, 2014
Eco147I AGGCCT 1 cut(s) 1734
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 2 cut(s) 1569, 1925
Eco32I GATATC 1 cut(s) 375
Eco47I GGWCC 2 cut(s) 1720, 2412
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 4 cut(s) 1610, 2736, 3014, 3470
Eco88I CYCGRG 2 cut(s) 708, 2066
Eco91I GGTNACC 1 cut(s) 2710
EcoICRI GAGCTC 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 2710
EcoRI GAATTC 3 cut(s) 117, 491, 3215
EcoRII CCWGG 5 cut(s) 446, 1170, 1540, 3052, 3442
EcoRV GATATC 1 cut(s) 375
EcoT14I CCWWGG 3 cut(s) 673, 1618, 2014
EcoT22I ATGCAT 3 cut(s) 281, 1485, 2896
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 3 cut(s) 673, 1618, 2014
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FauI CCCGC 2 cut(s) 614, 675
FauNDI CATATG 1 cut(s) 543
FblI GTMKAC 1 cut(s) 2842
Fnu4HI GCNGC 5 cut(s) 341, 456, 644, 873, 1493
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 5 cut(s) 341, 456, 644, 873, 1493
FspBI CTAG 6 cut(s) 198, 794, 1394, 2289, 2772, 2846
GlaI GCGC 1 cut(s) 71
GluI GCNGC 5 cut(s) 341, 456, 644, 873, 1493
GsaI CCCAGC 2 cut(s) 490, 1510
GsuI CTGGAG 1 cut(s) 469
HaeIII GGCC 6 cut(s) 169, 294, 792, 1734, 2241, 3442
HhaI GCGC 1 cut(s) 72
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 3 cut(s) 9, 580, 1638
HindII GTYRAC 3 cut(s) 9, 580, 1638
HindIII AAGCTT 1 cut(s) 2030
HphI GGTGA 7 cut(s) 115, 213, 2386, 2678, 2722, 3335, 3466
Hpy166II GTNNAC 8 cut(s) 9, 580, 1523, 1638, 1720, 2365, 2584, 2843
Hpy8I GTNNAC 8 cut(s) 9, 580, 1523, 1638, 1720, 2365, 2584, 2843
Hpy99I CGWCG 2 cut(s) 24, 2300
HpyCH4III ACNGT 6 cut(s) 1073, 1476, 1527, 2296, 2384, 3404
HpyCH4IV ACGT 3 cut(s) 1549, 2170, 2367
HpyF3I CTNAG 8 cut(s) 47, 465, 1689, 2148, 2258, 2648, 2723, 3069
HpySE526I ACGT 3 cut(s) 1549, 2170, 2367
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 70
LmnI GCTCC 2 cut(s) 869, 2672
Lsp1109I GCAGC 4 cut(s) 442, 655, 859, 1479
MaeI CTAG 6 cut(s) 198, 794, 1394, 2289, 2772, 2846
MaeII ACGT 3 cut(s) 1549, 2170, 2367
MaeIII GTNAC 5 cut(s) 511, 1381, 2245, 2693, 2710
MbiI CCGCTC 1 cut(s) 3391
MfeI CAATTG 3 cut(s) 1738, 2223, 2938
MflI RGATCY 5 cut(s) 200, 669, 1445, 1790, 1972
MhlI GDGCHC 2 cut(s) 366, 3186
MlyI GAGTC 4 cut(s) 203, 1755, 2080, 3477
MmeI TCCRAC 2 cut(s) 1488, 2137
Mph1103I ATGCAT 3 cut(s) 281, 1485, 2896
MroXI GAANNNNTTC 6 cut(s) 601, 813, 1083, 1515, 1609, 3036
MslI CAYNNNNRTG 3 cut(s) 596, 2708, 3087
MspCI CTTAAG 1 cut(s) 1786
MspR9I CCNGG 5 cut(s) 448, 1172, 1542, 3054, 3444
MunI CAATTG 3 cut(s) 1738, 2223, 2938
MvaI CCWGG 5 cut(s) 448, 1172, 1542, 3054, 3444
MvnI CGCG 1 cut(s) 70
NcoI CCATGG 1 cut(s) 673
NdeI CATATG 1 cut(s) 543
NlaIV GGNNCC 4 cut(s) 344, 604, 671, 2413
NmeAIII GCCGAG 1 cut(s) 2328
NmuCI GTSAC 3 cut(s) 1381, 2245, 2710
NsiI ATGCAT 3 cut(s) 281, 1485, 2896
NspI RCATGY 3 cut(s) 682, 1037, 3619
OliI CACNNNNGTG 1 cut(s) 2708
PaeI GCATGC 2 cut(s) 682, 3619
PaeR7I CTCGAG 2 cut(s) 708, 2066
PagI TCATGA 2 cut(s) 1567, 3367
PasI CCCWGGG 1 cut(s) 1171
PceI AGGCCT 1 cut(s) 1734
PcsI WCGNNNNNNNCGW 1 cut(s) 136
PdmI GAANNNNTTC 6 cut(s) 601, 813, 1083, 1515, 1609, 3036
PflMI CCANNNNNTGG 2 cut(s) 1624, 3193
PfoI TCCNGGA 1 cut(s) 3052
PkrI GCNGC 5 cut(s) 342, 457, 645, 874, 1494
PleI GAGTC 4 cut(s) 202, 1755, 2079, 3476
PpsI GAGTC 4 cut(s) 202, 1755, 2079, 3476
PshBI ATTAAT 2 cut(s) 2111, 3002
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 1 cut(s) 2170
Psp6I CCWGG 5 cut(s) 446, 1170, 1540, 3052, 3442
PspEI GGTNACC 1 cut(s) 2710
PspFI CCCAGC 2 cut(s) 486, 1506
PspGI CCWGG 5 cut(s) 446, 1170, 1540, 3052, 3442
PspN4I GGNNCC 4 cut(s) 344, 604, 671, 2413
PspPI GGNCC 5 cut(s) 168, 292, 1720, 2412, 3440
PspXI VCTCGAGB 1 cut(s) 708
PstI CTGCAG 1 cut(s) 2270
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 5 cut(s) 200, 669, 1445, 1790, 1972
RsaI GTAC 4 cut(s) 102, 2869, 3174, 3385
RsaNI GTAC 4 cut(s) 101, 2868, 3173, 3384
RseI CAYNNNNRTG 3 cut(s) 596, 2708, 3087
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 5 cut(s) 341, 456, 644, 873, 1493
Sau96I GGNCC 5 cut(s) 168, 292, 1720, 2412, 3440
SchI GAGTC 4 cut(s) 203, 1755, 2080, 3477
ScrFI CCNGG 5 cut(s) 448, 1172, 1542, 3054, 3444
SduI GDGCHC 2 cut(s) 366, 3186
SfcI CTRYAG 2 cut(s) 2266, 2568
Sfr274I CTCGAG 2 cut(s) 708, 2066
SinI GGWCC 2 cut(s) 1720, 2412
SlaI CTCGAG 2 cut(s) 708, 2066
SmiMI CAYNNNNRTG 3 cut(s) 596, 2708, 3087
SmlI CTYRAG 8 cut(s) 146, 708, 1756, 1786, 1961, 2066, 2777, 3257
SmoI CTYRAG 8 cut(s) 146, 708, 1756, 1786, 1961, 2066, 2777, 3257
SphI GCATGC 2 cut(s) 682, 3619
SseBI AGGCCT 1 cut(s) 1734
SspI AATATT 2 cut(s) 1335, 3064
SspMI CTAG 6 cut(s) 198, 794, 1394, 2289, 2772, 2846
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 1734
StyD4I CCNGG 5 cut(s) 446, 1170, 1540, 3052, 3442
StyI CCWWGG 3 cut(s) 673, 1618, 2014
TaaI ACNGT 6 cut(s) 1073, 1476, 1527, 2296, 2384, 3404
TaiI ACGT 3 cut(s) 1552, 2173, 2370
TaqI TCGA 6 cut(s) 709, 1632, 1767, 2067, 2298, 3264
TaqII GACCGA 2 cut(s) 153, 759
TatI WGTACW 3 cut(s) 100, 2867, 3172
TauI GCSGC 1 cut(s) 343
TscAI CASTG 4 cut(s) 2880, 3535, 3598, 3604
TseFI GTSAC 3 cut(s) 1381, 2245, 2710
TseI GCWGC 4 cut(s) 455, 643, 872, 1492
Tsp45I GTSAC 3 cut(s) 1381, 2245, 2710
TspGWI ACGGA 3 cut(s) 1170, 1622, 3461
TspRI CASTG 4 cut(s) 2880, 3535, 3598, 3604
Van91I CCANNNNNTGG 2 cut(s) 1624, 3193
Vha464I CTTAAG 1 cut(s) 1786
VpaK11BI GGWCC 2 cut(s) 1720, 2412
VspI ATTAAT 2 cut(s) 2111, 3002
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 3 cut(s) 682, 1037, 3619
XcmI CCANNNNNNNNNTGG 2 cut(s) 2012, 3089
XhoI CTCGAG 2 cut(s) 708, 2066
XmiI GTMKAC 1 cut(s) 2842
XmnI GAANNNNTTC 6 cut(s) 601, 813, 1083, 1515, 1609, 3036
XspI CTAG 6 cut(s) 198, 794, 1394, 2289, 2772, 2846
Zsp2I ATGCAT 3 cut(s) 281, 1485, 2896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.